PCP-B peptides and CrRLK1L receptor kinases control pollination via pH gating of aquaporins in Arabidopsis

IF 10.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell Pub Date : 2025-01-09 DOI:10.1016/j.devcel.2024.12.026

Zhiwen Liu, Xiaonan Chu, Weiwei Ren, Lijun Cheng, Chen Liu, Congcong Wang, Sihan Gao, Shaojun Dai, Chao Li

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Abstract

During pollen-stigma interaction, pollen coat protein B-class peptides (PCP-Bs) compete with stigmatic rapid alkalinization factor (RALF) for interaction with FERONIA/ANJEA receptor kinases (FER/ANJ), stimulating pollen hydration and germination. However, the molecular mechanism underlying PCP-Bs-induced, FER/ANJ-regulated compatible responses remains largely unknown. Through PCP-Bγ-induced phosphoproteomic analysis, we characterized a series of pollination-related signaling pathways regulated by FER/ANJ. Interestingly, on stigmatic papillary cells, pollen PCP-Bγ induced an elevation in cytosolic pH near the plasma membrane (PM), sustained by stigmatic RALF23/33 through regulation of the autoinhibited H⁺-ATPase 1/2 (AHA1/2) activity. We further found that RALFs/PCP-Bs and FER/ANJ regulated the pH alterations via phosphorylation of AHA1/2 C terminus. Furthermore, RALF23/33–FER/ANJ maintained the protonation of H197 in plasma membrane intrinsic proteins (PIPs), whereas PCP-B relieved the protonation through AHA activity. Altogether, this study reveals that pollen PCP-Bs trigger FER/ANJ-controlled compatible responses, particularly the opening of aquaporins via AHA-mediated pH changes, thereby facilitating pollen hydration in Arabidopsis.

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PCP-B肽和CrRLK1L受体激酶通过水通道蛋白的pH门控控制拟南芥授粉

在花粉与柱头相互作用过程中，花粉外壳蛋白b类肽（pcp - b）与柱头快速碱化因子（RALF）竞争与FERONIA/ANJEA受体激酶（FER/ANJ）相互作用，刺激花粉水化和萌发。然而，pcp - bs诱导的、FER/ anj调控的相容反应的分子机制仍然很大程度上未知。通过pcp - b - γ诱导的磷酸化蛋白质组学分析，我们表征了一系列由FER/ANJ调控的授粉相关信号通路。有趣的是，在柱头乳头状细胞上，花粉PCP-Bγ诱导质膜（PM）附近的胞质pH升高，通过调节自抑制的H+- atp酶1/2 （AHA1/2）活性，由柱头RALF23/33维持。我们进一步发现ralf /PCP-Bs和FER/ANJ通过磷酸化AHA1/2 C末端来调节pH的改变。此外，RALF23/33-FER /ANJ维持了H197在质膜固有蛋白（PIPs）中的质子化，而PCP-B通过AHA活性减轻了H197的质子化。总之，本研究表明，花粉PCP-Bs触发了FER/ anj控制的相容反应，特别是通过aha介导的pH变化打开水通道蛋白，从而促进了拟南芥花粉的水化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental cell 生物-发育生物学

CiteScore

18.90

自引率

1.70%

发文量

203

审稿时长

3-6 weeks

期刊介绍： Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.