工程技术最新文献

{"title":"Corrigendum to \"Development of a sustainable approach to produce galactaric acid from sunflower heads by engineered Pseudomonas putida KT2440\" [Bioresour. Technol. 437 (2025) 133115].","authors":"Huanghong Tan, Jie Hou, Jia Ouyang, Zhaojuan Zheng","doi":"10.1016/j.biortech.2025.133179","DOIUrl":"10.1016/j.biortech.2025.133179","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"437 ","pages":"133179"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of physical exercise on cognitive and motor function in patients with Alzheimer's disease: a meta-analysis based on randomized controlled trials.","authors":"Yuxin Gai, Xuelian Dai, Mengyi Qian, Guojian Lin, Piaorou Pan, Tianfu Dai, Yuedan Luo, Lijing Su","doi":"10.1007/s11571-025-10326-x","DOIUrl":"10.1007/s11571-025-10326-x","url":null,"abstract":"<p><p>This study investigated the effects of physical activity on cognitive and motor function in Alzheimer's disease patients. This study searched randomized controlled trials (RCTs) from PubMed, EMBASE, Science Direct, and Web of Science databases up to October 2024. The main evaluation tools were Mini-Mental State Examination (MMSE), Timed Up and Go Test (TUG), 6-Minute walk test (6MWT) and Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-cog). Mean difference (MD) with 95% confidence interval (CI) were calculated. A total of 25 randomized controlled trials involving 2213 participants were included. The MMSE score in exercise group was higher than that in control group (MD = 2.24, <i>p</i> = 0.002). Aerobic exercise (MD = 2.83, <i>p</i> = 0.01) and combined exercise (MD = 3.09, <i>p</i> = 0.03) in exercise group were significantly better than those in control group. There was no significant difference in strength exercise between the two groups (MD = 0.54, <i>p</i> = 0.48). At low intensity (MD = 5.75, <i>p</i> < 0.001) and moderate intensity (MD = 1.74, <i>p</i> = 0.008), MMSE scores in the exercise group were higher than those in the control group, whereas high-intensity exercise showed no benefit (MD = 0, <i>p</i> = 0.99). On the 6MWT scale, aerobic exercise scores were higher in the exercise group (MD = 51.55, <i>p</i> = 0.03), while there was no significant difference between the two groups under combined exercise (MD = 62.76, <i>p</i> = 0.45). The TUG scale (MD = -0.76, <i>p</i> = 0.06) and the ADAS-cog scale (MD = -1.99, <i>p</i> = 0.23) showed no significant difference between the two groups. Low intensity aerobic exercise improved cognitive and motor function in Alzheimer's disease patients, while strength exercise or high-impact exercise had little effect.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11571-025-10326-x.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"133"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global exponential stability of periodic solutions for Cohen-Grossberg neural networks involving generalized piecewise constant delay.","authors":"Kuo-Shou Chiu, Jyh-Cheng Jeng, Tongxing Li, Fernando Córdova-Lepe","doi":"10.1007/s11571-025-10315-0","DOIUrl":"10.1007/s11571-025-10315-0","url":null,"abstract":"<p><p>This paper investigates the global exponential stability and periodicity of the Cohen-Grossberg neural network model with generalized piecewise constant delay. By applying Schaefer's fixed-point theorem, a sufficient condition for the existence of periodic solutions in the model is established. Additionally, by constructing appropriate differential inequalities with generalized piecewise constant delay, sufficient conditions for the global exponential stability of the model are obtained. Finally, computer simulations are conducted to illustrate a globally exponentially stable periodic Cohen-Grossberg neural network model, thereby confirming the feasibility and effectiveness of the proposed results.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"129"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of beta band phase resetting in audio-visual temporal order judgment.","authors":"Yueying Li, Yasuki Noguchi","doi":"10.1007/s11571-024-10183-0","DOIUrl":"10.1007/s11571-024-10183-0","url":null,"abstract":"<p><p>The integration of auditory and visual stimuli is essential for effective language processing and social perception. The present study aimed to elucidate the mechanisms underlying audio-visual (A-V) integration by investigating the temporal dynamics of multisensory regions in the human brain. Specifically, we evaluated inter-trial coherence (ITC), a neural index indicative of phase resetting, through scalp electroencephalography (EEG) while participants performed a temporal-order judgment task that involved auditory (beep, A) and visual (flash, V) stimuli. The results indicated that ITC phase resetting was greater for bimodal (A + V) stimuli compared to unimodal (A or V) stimuli in the posterior temporal region, which resembled the responses of A-V multisensory neurons reported in animal studies. Furthermore, the ITC got lager as the stimulus-onset asynchrony (SOA) between beep and flash approached 0 ms. This enhancement in ITC was most clearly seen in the beta band (13-30 Hz). Overall, these findings highlight the importance of beta rhythm activity in the posterior temporal cortex for the detection of synchronous audiovisual stimuli, as assessed through temporal order judgment tasks.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11571-024-10183-0.</p>","PeriodicalId":10500,"journal":{"name":"Cognitive Neurodynamics","volume":"19 1","pages":"28"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>ZmNF-YB10</i>, a maize NF-Y transcription factor, positively regulates drought and salt stress response in <i>Arabidopsis thaliana</i>.","authors":"Yimeng Wang, Peng Jiao, Chenyang Wu, Chunlai Wang, Ke Shi, Xiaoqi Gao, Shuyan Guan, Yiyong Ma","doi":"10.1080/21645698.2024.2438421","DOIUrl":"https://doi.org/10.1080/21645698.2024.2438421","url":null,"abstract":"<p><p>Maize (<i>Zea mays</i> L.) is a major food and feed crop and an important raw material for energy, chemicals, and livestock. The NF-Y family of transcription factors in maize plays a crucial role in the regulation of plant development and response to environmental stress. In this study, we successfully cloned and characterized the maize NF-Y transcription factor gene <i>ZmNF-YB10</i>. We used bioinformatics, quantitative fluorescence PCR, and other techniques to analyze the basic properties of the gene, its tissue expression specificity, and its role in response to drought, salt, and other stresses. The results indicated that the gene was 1209 base pairs (bp) in length, with a coding sequence (CDS) region of 618 bp, encoding a polypeptide composed of 205 amino acid residues. This polypeptide has a theoretical isoelectric point of 5.85 and features a conserved structural domain unique to the NF-Y family. Quantitative fluorescence PCR results demonstrated that the <i>ZmNF-YB10</i> gene was differentially upregulated under drought and salt stress treatments but exhibited a negatively regulated expression pattern under alkali and cold stress treatments. Transgenic <i>Arabidopsis thaliana</i> subjected to drought and salt stress in soil showed greener leaves than wild-type <i>A. thaliana</i>. In addition, the overexpression lines showed reduced levels of hydrogen peroxide (H₂O₂), superoxide (O^2-), and malondialdehyde (MDA) and increased activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Western blot analysis revealed a distinct band at 21.8 kDa. Salt and drought tolerance analyses conducted in <i>E. coli</i> BL21 indicated a positive regulation. In yeast cells, <i>ZmNF-YB10</i> exhibited a biological function that enhances salt and drought tolerance. Protein interactions were observed among the <i>ZmNF-YB10</i>, <i>ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes. It is hypothesized that the <i>ZmNF-YB10, ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes may play a role in the response to abiotic stresses, such as drought and salt tolerance, in maize.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"28-45"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/21691401.2025.2473244","DOIUrl":"https://doi.org/10.1080/21691401.2025.2473244","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"87"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human tendon stem/progenitor cell-derived extracellular vesicle production promoted by dynamic culture.","authors":"Marta Clerici, Maria Camilla Ciardulli, Erwin Pavel Lamparelli, Joseph Lovecchio, Emanuele Giordano, Tina P Dale, Nicholas R Forsyth, Nicola Maffulli, Giovanna Della Porta","doi":"10.1080/21691401.2025.2475099","DOIUrl":"10.1080/21691401.2025.2475099","url":null,"abstract":"<p><p>Tendon injuries significantly impact quality of life, prompting the exploration of innovative solutions beyond conventional surgery. Extracellular Vesicles (EVs) have emerged as a promising strategy to enhance tendon regeneration. In this study, human Tendon Stem/Progenitor Cells (TSPCs) were isolated from surgical biopsies and cultured in a Growth-Differentiation Factor-5-supplemented medium to promote tenogenic differentiation under static and dynamic conditions using a custom-made perfusion bioreactor. Once at 80% confluence, cells were transitioned to a serum-free medium for conditioned media collection. Ultracentrifugation revealed the presence of vesicles with a 10⁶ particles/mL concentration and sub-200nm diameter size. Dynamic culture yielded a 3-fold increase in EV protein content compared to static culture, as confirmed by Western-blot analysis. Differences in surface marker expression were also shown by flow cytometric analysis. Data suggest that we efficiently developed a protocol for extracting EVs from human TSPCs, particularly under dynamic conditions. This approach enhances EV protein content, offering potential therapeutic benefits for tendon regeneration. However, further research is needed to fully understand the role of EVs in tendon regeneration.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"1-16"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The integration of metabolites from <i>Forsythia suspensa</i> and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies.","authors":"Yanni Wang, Xiangxiang Peng, Bingjie Qian, Libo Wang, Jiabing Wang","doi":"10.1080/21691401.2025.2475088","DOIUrl":"10.1080/21691401.2025.2475088","url":null,"abstract":"<p><p>This study integrates metabolites from Forsythia suspensa (FS) and gut microbiota GM to assess combined therapeutic efficacy against drug-induced liver injury (DILI) using network pharmacology and molecular docking. Metabolites of FS and GM were retrieved from the NPASS and gutMGene databases, respectively. Relevant targets for metabolites and DILI-related targets were identified through public databases. The PPI network and KEGG pathway analysis were employed to identify hub targets and key signalling pathways. Furthermore, we performed a molecular docking assay on the active metabolites and targets to verify the network pharmacological concept. The physicochemical properties and toxicity of identified key metabolites were assessed using in silico platforms. 19 final targets were recognized as key proteins responsible for the alleviation of DILI by FS and GM metabolites, with ESR1 emerging as a central target in the PPI network. The estrogen signalling pathway, particularly involving ESR1, ESR2 and JUN genes, was identified as a key mediator in the therapeutic effects. Four GM metabolites (baicalein, luteolin, lunularin and 2,3-bis(3,4-dihydroxybenzyl)butyrolactone) and two FS metabolites (pinoresinol and isolariciresinol) were identified as non-toxic, promising candidates. In conclusion, metabolites from FS and GM may exert a potent synergistic effect on DILI through modulation of the estrogen signalling pathway.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"105-121"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanistic action of mogroside V in the alleviation of oxidative aging.","authors":"Qiannan Wang, Xinyue Mao, Yulan Li, Gang Mo, Dayu Li, Deping Cao, Gen Chen","doi":"10.1080/21691401.2025.2486752","DOIUrl":"https://doi.org/10.1080/21691401.2025.2486752","url":null,"abstract":"<p><strong>Introduce: </strong>Diseases related to oxidative ageing are becoming increasingly evident in younger individuals. In this study, we investigated the mechanisms underlying the actions of mogroside V when used to treat anti-oxidative ageing.</p><p><strong>Methods: </strong>We used D-galactose-induced LO2 cells and C57BL/6J mice as models to investigate the molecular mechanisms of mogroside V (MV) for the treatment of oxidative ageing. Network pharmacology was used to predict the targets of MV for the treatment of oxidative ageing.</p><p><strong>Results: </strong>By down-regulating the <i>EGFR</i>/<i>p38</i>/<i>JNK</i> pathway, MV significantly inhibited oxidative ageing and apoptosis in cells, reduced the levels of SA-β-galactosidase. In mice, compared with the model group, MV treatment (100 mg/kg·d) reduced MDA levels and significantly increased the levels of GSH and SOD; furthermore, the size and structure of the liver leaflet and glomeruli was arranged in a regular manner; the small intestine glands had decreased in size. Moreover, the expression levels of <i>Ptp1b</i> mRNA had increased significantly while the levels of <i>c-Jun</i> mRNA and protein were significantly reduced. MV also increased the proportion of beneficial bacteria in the small intestine, including <i>Bacteroidales</i> and <i>Lactobacillaceae</i>.</p><p><strong>Conclusion: </strong>Our analyses revealed that MV can significantly reduce oxidative ageing caused by the accumulation of D-galactose.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"53 1","pages":"166-180"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of type II chalcone isomerase (<i>CHI</i>) genes in regulating soybean (<i>Glycine max L</i>.) nodule formation.","authors":"Xinyue Wang, Jingwen Li, Yuxue Zhou, Jinhao Zhang, Le Wang, Yajing Liu, Xuguang Yang, Hongshuang Han, Qingyu Wang, Ying Wang","doi":"10.1080/21645698.2025.2486280","DOIUrl":"10.1080/21645698.2025.2486280","url":null,"abstract":"<p><p>Biological nitrogen fixation (BNF) is the most cost-effective and environmentally benign method for nitrogen fertilization. Isoflavones are important signaling factors for BNF in leguminous plants. Whether chalcone isomerase (<i>CHI</i>), the key enzyme gene in the flavonoid synthesis pathway, contributes to soybean (<i>Glycine max</i>) nodulation has not yet been fully clarified. In the present study, we identified the functions of three types of <i>GmCHI</i> for BNF using a hairy root system. The results showed that <i>GmCHI1A</i> and <i>GmCHI1B1</i> positively increased nodulation while <i>GmCHI1B2</i> did not, with the <i>GmCHI1A</i> gene having a greater effect than <i>GmCHI1B1</i>. Meanwhile, the daidzein and genistein contents were significantly increased in composite plants overexpressing <i>GmCHI1A</i> and reduced in composite plants, thus interfering with <i>GmCHI1A</i>. However, overexpression of <i>GmCHI1B1</i> significantly increased the content of glycitein but not daidzein, genistein content implied that homologous genes exhibit functional differentiation. These results provide a reference for subsequent studies on improving nitrogen fixation in soybeans and providing functional genes for the improvement of new varieties.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"305-317"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}