工程技术最新文献_第10页

Peptides-functionalized gold nanostars enhanced degradation of PD-L1 for improved prostate cancer immunotherapy.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-24 DOI: 10.1177/08853282251319473

Liangjun Tao, Yifei Zhang, Jingwei Zhang, Jianping Tao, Yu Gong, Jun Mao, Qixing Tian, Ping Ao, Dong Zhuo

{"title":"Peptides-functionalized gold nanostars enhanced degradation of PD-L1 for improved prostate cancer immunotherapy.","authors":"Liangjun Tao, Yifei Zhang, Jingwei Zhang, Jianping Tao, Yu Gong, Jun Mao, Qixing Tian, Ping Ao, Dong Zhuo","doi":"10.1177/08853282251319473","DOIUrl":"10.1177/08853282251319473","url":null,"abstract":"Blockage of the interaction between programmed death receptor-1 (PD-1) and programmed death ligand-1 (PD-L1) can restore T-cell activity and enhance antitumor immunity. PD-1/PD-L1 pathway inhibitors have promising applications in the treatment of advanced prostate cancer (PCa). We successfully developed a peptides-functionalized gold nanoconstruct (P-AuNS) consisted of PD-L1-binding peptide (PD-L1pep, P) and gold nanostar (AuNS), which could bind to cell-surface PD-L1 specifically and deliver PD-L1 into PCa cells with high efficiency. In PCa cells, P-AuNS can efficiently degrade PD-L1 in a lysosomal-dependent manner. In the co-culture system of Jurkat cells and DU145 cells, P-AuNS restored the proliferative capacity and interferon-gamma (IFN-γ) secretion level of Jurkat cells inhibited by co-cultured DU145 cells, indicating that P-AuNS effectively hampered the interaction between PD-1 and PD-L1. In addition, in PCa-bearing mice, P-AuNS can effectively inhibit tumor growth and down-regulate PD-L1 protein levels, and in vivo experimental results show that P-AuNS has no systemic toxicity. P-AuNS block the interaction between PD-1 and PD-L1 by efficiently degrading PD-L1, thus restoring the antitumor activity of T cells and inhibiting tumor progression of PCa. In all, P-AuNS has great promise as a potential immunotherapy strategy in the treatment of advanced PCa and even other solid tumors.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1188-1201"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High α-lipoic acid-loaded hollow mesoporous prussian blue nanozymes for targeted therapy of nasopharyngeal carcinoma in mice.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-05 DOI: 10.1177/08853282251318514

Ya Pan, Xiaofeng Wang, Xuejun Zhou, Haipeng Chen, Yuxia Zou

{"title":"High α-lipoic acid-loaded hollow mesoporous prussian blue nanozymes for targeted therapy of nasopharyngeal carcinoma in mice.","authors":"Ya Pan, Xiaofeng Wang, Xuejun Zhou, Haipeng Chen, Yuxia Zou","doi":"10.1177/08853282251318514","DOIUrl":"10.1177/08853282251318514","url":null,"abstract":"This study successfully constructs a tumor-targeting α-lipoic acid-loaded hollow mesoporous prussian blue nanozyme (AHPRzyme) for targeted therapy of nasopharyngeal carcinoma in mice. In these nanozymes, Arg-Gly-Asp (RGD) acts as a targeting ligand, enabling effective targeting of tumor cells. Additionally, AHPRzyme exhibits multiple anti-tumor mechanisms: ① The prussian blue nanozymes in AHPRzyme have catalase (CAT) activity, which decomposes H2O2 in human nasopharyngeal carcinoma CEN2 cells into non-toxic H2O, reducing H2O2 levels and minimizing damage to normal cells. The released O2 helps alleviate the hypoxic environment of the tumor, inhibiting lactate production due to hypoxia and consequently suppressing tumor growth. ② The prussian blue nanozymes also have peroxidase (POD) activity, which catalyzes H2O2 in tumor cells to generate ·OH, a reactive oxygen species, leading to tumor cell apoptosis. ③ The α-lipoic acid structure in AHPRzyme contains disulfide bonds that react with GSH, depleting excess glutathione (GSH) in tumor cells, disrupting the oxidative stress balance within the cells, and making them more sensitive to reactive oxygen species, thereby increasing tumor cell apoptosis. In summary, AHPRzyme can inhibit tumor cell growth and promote tumor cell apoptosis by improving the tumor microenvironment, achieving the goal of anti-nasopharyngeal carcinoma therapy.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1177-1187"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on intervertebral disc repair strategies and mechanisms based on hydrogel.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-10 DOI: 10.1177/08853282251320227

Zekun Hua, Yinuo Zhao, Meng Zhang, Yanqin Wang, Haoyu Feng, Xiaochun Wei, Xiaogang Wu, Weiyi Chen, Yanru Xue

{"title":"Research progress on intervertebral disc repair strategies and mechanisms based on hydrogel.","authors":"Zekun Hua, Yinuo Zhao, Meng Zhang, Yanqin Wang, Haoyu Feng, Xiaochun Wei, Xiaogang Wu, Weiyi Chen, Yanru Xue","doi":"10.1177/08853282251320227","DOIUrl":"10.1177/08853282251320227","url":null,"abstract":"Intervertebral disc degeneration (IDD) arises from a complex interplay of genetic, environmental, and age-related factors, culminating in a spectrum of low back pain (LBP) disorders that exert significant societal and economic impact. The present therapeutic landscape for IDD poses formidable clinical hurdles, necessitating the exploration of innovative treatment modalities. The hydrogel, as a biomaterial, exhibits superior biocompatibility compared to other biomaterials such as bioceramics and bio-metal materials. It also demonstrates mechanical properties closer to those of natural intervertebral discs (IVDs) and favorable biodegradability conducive to IVD regeneration. Therefore, it has emerged as a promising candidate material in the field of regenerative medicine and tissue engineering for treating IDD. Hydrogels have made significant strides in the field of IDD treatment. Particularly, injectable hydrogels not only provide mechanical support but also enable controlled release of bioactive molecules, playing a crucial role in mitigating inflammation and promoting extracellular matrix (ECM) regeneration. Furthermore, the ability of injectable hydrogels to achieve minimally invasive implantation helps minimize tissue damage. This article initially provides a concise exposition of the structure and function of IVD, the progression of IDD, and delineates extant clinical interventions for IDD. Subsequently, it categorizes hydrogels, encapsulates recent advancements in biomaterials and cellular therapies, and delves into the mechanisms through which hydrogels foster disc regeneration. Ultimately, the article deliberates on the prospects and challenges attendant to hydrogel therapy for IDD.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1121-1142"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable ADM temperature-sensitive hydrogel loaded with bFGF in diabetic rat wound healing study.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-18 DOI: 10.1177/08853282251321943

Haojiao Chen, Jianping Sun, Wenyang Liu

{"title":"Injectable ADM temperature-sensitive hydrogel loaded with bFGF in diabetic rat wound healing study.","authors":"Haojiao Chen, Jianping Sun, Wenyang Liu","doi":"10.1177/08853282251321943","DOIUrl":"10.1177/08853282251321943","url":null,"abstract":"Background: Diabetic wound is one of the most common diabetic chronic complications. Effective treatments of diabetic wound remain limited. Here, we explored the effects of basic fibroblast growth factor (bFGF)-acellular dermal matrix (ADM) hydrogel on the diabetic wound. Methods: The bFGF-ADM hydrogel was manufactured by mixing 180 µL ADM hydrogel and 20 µL bFGF aqueous solution (10 mg/mL). The morphology of ADM hydrogel and bFGF-ADM hydrogel was observed under scanning electron microscope. The release property of bFGF-ADM hydrogel was determined by ELISA. CCK-8 assay was utilized to estimate the cell viability of mouse skin fibroblasts. The diabetes mellitus (DM) model was established in rats. The four wounds on the back of each DM rat were treated with the ADM hydrogel, bFGF-ADM hydrogel, bFGF aqueous solution and no solution (control), respectively. The wound healing rate of each rat was estimated. The traumatized skin tissue of each rat was observed by H&E staining and Sirius red staining. Results: The bFGF-ADM hydrogel displayed an interconnected pore structure and bFGF was gradually released from the bFGF-ADM hydrogel over time. The bFGF-ADM hydrogel could enhance the cell viability of skin fibroblasts and promote the wound healing rate, the re-epithelialization of wound and increase the collagen fiber content of dermis. And the bFGF-ADM hydrogel exhibited better therapeutic effects of diabetic wound than either bFGF or ADM alone. Conclusions: Our study revealed that the bFGF-ADM hydrogel could promote diabetic wound healing.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1156-1164"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dexamethasone loaded blend poly hydroxybutyrate-stearic acid nanoparticles for ocular inflammation.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-27 DOI: 10.1177/08853282251324619

Ebru Erdal, Nagihan Uğurlu, Esin Akbay Çetin, Nelisa Laçin Türkoğlu, Cafer Çakal, Murat Demirbilek

{"title":"Dexamethasone loaded blend poly hydroxybutyrate-stearic acid nanoparticles for ocular inflammation.","authors":"Ebru Erdal, Nagihan Uğurlu, Esin Akbay Çetin, Nelisa Laçin Türkoğlu, Cafer Çakal, Murat Demirbilek","doi":"10.1177/08853282251324619","DOIUrl":"10.1177/08853282251324619","url":null,"abstract":"The aim of this study is to develop and analyze dexamethasone-loaded poly hydroxybutyrate-stearic acid blend nanoparticles for the treatment of non-bacterial uveitis. Uveitis is a chronic inflammatory eye disease responsible for 10-15% of global blindness. While repeated intravitreal steroid injection is a successful treatment strategy, it has drawbacks such as cataracts and retinal detachment. Serious side effects and high costs caused by recurrent infections are important limiting factors. Controlled release systems are preferred to maintain therapeutic drug concentrations in the vitreous humor while minimizing the frequency of injections. In this study, PHB-stearic acid blend nanoparticles with varying ratios of dexamethasone suitable for intravitreal injection were fabricated. It was assessed the effects of the stearic acid ratio on loading efficiency, release profiles, size distribution, and thermal properties. The results indicated that an increase in the stearic acid ratio led to a reduction in nanoparticle diameter and an enhancement in the dexamethasone loading capacity. Additionally, higher stearic acid ratios resulted in a decrease in the melting enthalpy of the nanoparticles, which expedited dexamethasone release. Importantly, the nanoparticle formulations exhibited no toxicity to eye endothelial cells or THP-1 cells.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1211-1221"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of dual drug loaded magnetic gold nanohybrid system for osteoarthritis.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-03-03 DOI: 10.1177/08853282251324352

Gul Rehman, Jadoon Khan, May M Alrashed, Muhammad Abbas, Masood Ur Rehman, Tawaf Ali Shah, Kotb A Attia, Arif Ahmed Mohammed

{"title":"Development and characterization of dual drug loaded magnetic gold nanohybrid system for osteoarthritis.","authors":"Gul Rehman, Jadoon Khan, May M Alrashed, Muhammad Abbas, Masood Ur Rehman, Tawaf Ali Shah, Kotb A Attia, Arif Ahmed Mohammed","doi":"10.1177/08853282251324352","DOIUrl":"10.1177/08853282251324352","url":null,"abstract":"Cartilage deterioration in patients with osteoarthritis presents a significant challenge, primarily attributable to the inadequate oral bioavailability and poor dosage compliance of chondroprotective agents. The Chondroitin Sulphate (CS) is a stabilizing and reducing agent for metal NP as well as homing agent by binding to surface molecules (CD44, L-selectin, P-selectin, and annexin-6) of chondrocytes at the OA site. This study was designed to develop intra-articular magnetic gold nanohybrids for the co-delivery of chondroitin sulfate, glucosamine sulfate, and gold, aiming to achieve synergistic anti-inflammatory and cartilage regenerative effects and in vitro assessments of drug release were conducted. Additionally, in animal study, the male albino rats underwent anesthesia by inhaling isoflurane using the open-drop exposure method, and chondrocytes were then harvested for cytotoxicity and biocompatibility assays. Physical characterization revealed 66 nm particle size with uniform distribution and colloidal stability of MGN-CS-GS. Zeta potential and FTIR analysis showed electrostatic interaction between the carboxyl and amino groups of MGN-CS and GS. VSM and EDX confirmed paramagnetic and core-shell characteristics of nanohybrids, respectively. It was found that the MGN-CS-GS released more CS (72%) and GS (85%) at acidic pH with continuous release pattern, which will improve patient compliance. The nanohybrid's cytotoxicity assay demonstrated excellent biocompatibility and cellular viability of OA chondrocytes triggered by interleukin-1β (IL-1β) compared to marketed formulation. The results demonstrated that MGN-CS-GS continuously released both drugs with high biocompatibility and cellular viability of OA chondrocytes. The successful synthesis of MGN-CS-GS is a foundation for further research on its potential application as a novel co-drug carrier nanohybrid system.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1222-1239"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of macrophage cell lines confined with silica and/or silicon particles and embedded in structured collagen gels.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-11 DOI: 10.1177/08853282251319875

Pedro U Muñoz-González, Jorge Delgado, Gerardo González-García, Birzabith Mendoza-Novelo

{"title":"Stimulation of macrophage cell lines confined with silica and/or silicon particles and embedded in structured collagen gels.","authors":"Pedro U Muñoz-González, Jorge Delgado, Gerardo González-García, Birzabith Mendoza-Novelo","doi":"10.1177/08853282251319875","DOIUrl":"10.1177/08853282251319875","url":null,"abstract":"Macrophages encapsulated in composite gels are subjected to a three-dimensional (3D) microenvironment and material-related stimuli that allow modulation of their phenotypes. Herein, 3D collagen fibrillar networks structured with di- or tri-functionalized oligourethanes, including Si-O or Si-Si particles confined therein, are compared regarding their physicochemical properties and material-guided macrophage activation. Gelation kinetics, degradation/swelling, and rheometric results demonstrated that the properties of the composite gels depend on the oligourethane functionalization number (derived from diols/triols and L-Lysine diisocyanate, LDI) and silica incorporation. Human or murine macrophages seeded or encapsulated in the composite gels showed good viability and the adoption of an anti-inflammatory phenotype in response to the silica in the composite gel, showing accelerated gelation when cell culture components are present in the liquid precursors. An increase in cell viability proportional to the storage modulus was observed. ELISA tests strongly suggest that the Si-Si nanoparticles in the composites can antagonize the pro-inflammatory stimulation with lipopolysaccharides (LPS) and interferon-gamma (IFNγ), even promoting an anti-inflammatory response in embedded cells after 24 h. Silicon-doped and crosslinked collagen gels have good potential to modulate macrophage inflammatory response, serving as a 3D immunomodulatory scaffold.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1240-1257"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baicalin loaded monodispersed polycaprolactone (PCL) microspheres preparation by polyvinylidene fluoride (PVDF) membrane emulsification for wound healing in dermal fibroblasts. 利用聚偏二氟乙烯（PVDF）膜乳化法制备负载黄芩苷的单分散聚己内酯（PCL）微球，用于真皮成纤维细胞的伤口愈合。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-13 DOI: 10.1177/08853282251321259

Wenwei Zhong, Xiang Cao, Haoran Zeng, Manqun Tang, Guizhen Li, Jia Xiong, Yunong Wang, Liwei Guo, Jing Ji

{"title":"Baicalin loaded monodispersed polycaprolactone (PCL) microspheres preparation by polyvinylidene fluoride (PVDF) membrane emulsification for wound healing in dermal fibroblasts.","authors":"Wenwei Zhong, Xiang Cao, Haoran Zeng, Manqun Tang, Guizhen Li, Jia Xiong, Yunong Wang, Liwei Guo, Jing Ji","doi":"10.1177/08853282251321259","DOIUrl":"10.1177/08853282251321259","url":null,"abstract":"The development of injectable bio-stimulating polycaprolactone (PCL) microspheres for wound healing has strict requirement on size and morphology control, particularly favoring microspheres within the range between 20-50 µm. PCL microspheres with smaller sizes are phagocyted at rapid rate while larger microspheres could cause inflammation. Homogenization can be regarded as an irreversible process to generate microspheres of particular size range while it still remains as the most common approach for microspheres production. Membrane emulsification technology shows great potential in fine tailoring of microspheres while still holds promising ability for scale-up production. Membranes with uniform large pores and dual hydrophilicity might be capable of the production of large microspheres via emulsification with tailorable size distribution. The aim of this study is to verify the feasibility of PVDF membranes with large pores on the generation of PCL microspheres via the combined crystallization diffusion (CCD) approach. The effect of dope solution concentration and PVDF molecular weights on membrane morphologies and the corresponding microspheres characteristics were investigated. Results showed that concentration of 20 wt% produced microspheres at desirable size of 24.14 µm and the optimal span of 0.53. Microspheres with narrow distribution showed the highest drug loading efficiency of baicalin at 8.42 %. The baicalin loaded PCL microspheres presented gradual release of drug release over 33-day of in vitro testing and significantly improved cell growth rate of 111.67 % as compared to the ones prepared by homogenization approach. The wound healing ability was enhanced after the treatment of baicalin-loaded PCL microspheres as compared to empty loaded PCL microspheres.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1143-1155"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical characterization of PEGylated exosome constructs: Size, charge, and morphology changes in non-specific alkylating N-terminal reactions.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-25 DOI: 10.1177/08853282251323198

Andrés Martínez-Santillán, José González-Valdez

{"title":"Physical characterization of PEGylated exosome constructs: Size, charge, and morphology changes in non-specific alkylating N-terminal reactions.","authors":"Andrés Martínez-Santillán, José González-Valdez","doi":"10.1177/08853282251323198","DOIUrl":"10.1177/08853282251323198","url":null,"abstract":"Small extracellular vesicles, commonly referred to as exosomes, withhold a promising future in the pharmaceutical industry as carriers for targeted drug delivery due to their high specificity and bioavailability when compared to synthetic-based vectors. They, however, present some limitations for systematic administration because of natural organism defenses and their high-water solubility, ultimately making it difficult for them to reach the intended target. To improve the delivery capacity of these nanoparticles, the possibility for the construction of PEGylated versions was explored in this work. This process was performed, analyzed, and characterized using N-terminal specific PEGylation reactions targeted to the protein contents in the exosomal membrane. For this, two different mono-methoxy polyethylene glycols (mPEG) of 5 and 20 kDa were reacted with exosomes under alkylating conditions. The resulting 5k and 20k PEGylated exosome constructs were characterized and compared with unmodified exosomes, using size, morphology, and zeta potential as comparison parameters. Results after analysis showed an absorbance reduction of approximately 65% and 34% (for the 5 and 20 kDa conjugates respectively), a reduction of 10 to 20% in peak resolution, particle size increase corresponding to the polymer sizes used, and a slight reduction in electric distribution of about 2 to 3 mV less than the unmodified vesicles. The data obtained may provide insights for the optimization of exosome PEGylation strategies for therapeutic use.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1202-1210"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smoothed Particle Hydrodynamics-based geometric modeling of lattice structures with controllable manifold nodes

IF 10.3 1区工程技术

Additive manufacturing Pub Date : 2025-04-05 DOI: 10.1016/j.addma.2025.104744

Zhuangyu Li, Ziqi Zhou, Wenlei Xiao, Gang Zhao, Changri Xiong

{"title":"Smoothed Particle Hydrodynamics-based geometric modeling of lattice structures with controllable manifold nodes","authors":"Zhuangyu Li, Ziqi Zhou, Wenlei Xiao, Gang Zhao, Changri Xiong","doi":"10.1016/j.addma.2025.104744","DOIUrl":"10.1016/j.addma.2025.104744","url":null,"abstract":"<div><div>The advancement of additive manufacturing has necessitated the development of high-performance geometric modeling methods for lattice structures. A critical challenge in current additive manufacturing technologies is effectively controlling the geometric shape of nodes within lattice structures to achieve specific functional enhancements, such as strengthening mechanical properties, thereby reducing stress concentration issues. This study presents a novel geometric modeling method employing Smoothed Particle Hydrodynamics (SPH) to smoothly control the geometric shape of nodes in lattice structures, suitable for variable-radius, heterogeneous configurations. This method is named SPH-based Lattice Geometric Modeling (SLGM). The SLGM comprises two primary steps: initially representing the geometric form around each node using a particle set, followed by the application of the SPH Particle Dynamics Model (SPDM) to control the geometric shape of each node in parallel. The method was applied to several intricate lattice structure designs, confirming its extensive broad applicability. Two optimized lattice-based beams were designed and subjected to three-point bending tests. The results showed a significant enhancement in structural load-bearing capacity for both models, with different levels of improvement, demonstrating the effectiveness of the proposed geometric modeling method. The modeling algorithm features high parallelism, making it suitable for generating large-scale models. This method provides effective strategies for structural optimization and efficient resource utilization in additive manufacturing, with the potential to advance the technology’s application in high-performance domains.</div></div>","PeriodicalId":7172,"journal":{"name":"Additive manufacturing","volume":"103 ","pages":"Article 104744"},"PeriodicalIF":10.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0