BioEnergy Research最新文献

{"title":"Soil Carbon Dioxide Equivalent Emissions and Theoretical Ethanol Yield from Midwestern Bioenergy Systems","authors":"Frank E. Johnson II,&nbsp;Adebukola O. Dada,&nbsp;Shalamar D. Armstrong,&nbsp;Douglas R. Smith,&nbsp;Jeffrey J. Volenec,&nbsp;Sylvie M. Brouder","doi":"10.1007/s12155-025-10832-0","DOIUrl":"10.1007/s12155-025-10832-0","url":null,"abstract":"<div><p>There has been a significant demand to increase biofuel production and usage in the USA. Bioenergy crops also have the potential to mitigate greenhouse gas (GHG) emissions from agricultural systems but crop type (annual vs. perennial) may impact mitigation potential. Objectives of this research were to (1) assess cumulative carbon dioxide equivalence (CO₂eq) of annual and perennial crops, (2) evaluate the theoretical ethanol yield (TTEY) from annual and perennial crops, and (3) assess the CO₂eq/TTEY ratio (CTR) of annual and perennial crops. This study was conducted from 2008 to 2016 at the Purdue University Water Quality Field Station near West Lafayette, IN. Replicated treatments (<i>n</i> = 4) included continuous corn (CC), continuous sorghum (CS), Miscanthus (MS), switchgrass (SG), and restored prairie (RP). This study calculated CO₂eq using CO₂ and N₂O emissions. To estimate TTEY, yield of stover carbohydrate pools, recovery efficiencies of sugars from cell wall polysaccharides, fermentation efficiency of sugars to ethanol, the theoretical ethanol yield from sugars, and grain (for corn and sorghum) were used. Averaging over the course of the study, the highest annual CO₂eq observed were 7.8 and 6.7 Mg CO₂-C ha⁻¹ year⁻¹ from RP and SG, respectively. Average CO₂eq for CC, CS, and MS were 5.8, 5.5, and 5.0 Mg CO₂-C ha⁻¹ year⁻¹, respectively. The highest and lowest TTEY were observed in MS and RP, yielding 189.4 and 26.7 GJ EtOH ha⁻¹ year⁻¹, respectively. Thus, MS had the lowest CTR out of these treatments. Averaging across plant type, CO₂eq, TTEY, and CTR were 6.5 Mg CO₂-C ha⁻¹ year⁻¹, 89.8 GJ EtOH ha⁻¹ year⁻¹, and 345 kg CO₂-C GJ⁻¹ EtOH, respectively, for perennials, and 5.7 Mg CO₂-C ha⁻¹ year⁻¹, 118 GJ EtOH ha⁻¹ year⁻¹, and 52.1 kg CO₂-C GJ⁻¹ EtOH, respectively, for annuals. Although these results suggest lower CO₂eq, higher TTEY, and lower CTR for annual crops compared to perennials, the mitigation potential of emitted GHGs from perennials in agricultural systems used for biofuel production is also highlighted.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10832-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food Waste Devolatilization Kinetics with Demonstration of Its Implementation in Computational Modeling of a Fluidized Bed Pyrolysis Reactor","authors":"Nihal Yasir,&nbsp;Yassir Makkawi,&nbsp;Baraa Ahmed,&nbsp;Ondrej Masek","doi":"10.1007/s12155-025-10835-x","DOIUrl":"10.1007/s12155-025-10835-x","url":null,"abstract":"<div><p>This study presents the derivation of a kinetic model for the devolatilization of post-consumption food waste using thermogravimetric analysis (TGA). The derived model was implemented in an Eulerian-Eulerian Computational Fluid Dynamics (CFD) framework to simulate pyrolysis in a fluidized bed reactor operating at 550 °C. Due to the heterogeneity and complexity of food waste composition, the reaction function and devolatilization kinetics were identified using a staged decomposition approach. As determined by TGA, these stages correspond to the decomposition of protein, cellulose, and hemicellulose approximately between 270 and 300 °C, and the decomposition of lipids approximately between 350 and 400 °C. The activation energy, obtained using three different model-free iso-conversional methods, was consistent, with an average value of <span>({E}_{a})</span> = 219.23 kJ/mol. The pyrolysis reaction was found to follow an order-based model, with the master plot method yielding an average reaction order of <span>(n)</span> = 10.3 and an Arrhenius frequency factor of <span>(A)</span> = 1.16 × 10¹⁹ [(kmol/m³)^1−<i>n</i>/s]. The predicted distribution of pyrolysis products, validated against experimental data, highlights the robustness of the proposed analysis and its computational implementation. This methodology provides a strong foundation for further development and adaptation to simulate the pyrolysis of food waste and other diverse feedstocks, broadening its applicability to various types of biomass.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10835-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrolysis of Hass Avocado (Persea americana) Seeds: Kinetic and Economic Analysis of Bio-oil, Gas, and Biochar Production","authors":"Dionisio Humberto Malagón-Romero,&nbsp;Nazly Dayanna León-Caballero,&nbsp;Marco Antonio Velasco-Peña,&nbsp;Juan Pablo Arrubla-Vélez,&nbsp;Myriam Quintero-Naucil,&nbsp;Valentina Aristizábal-Marulanda","doi":"10.1007/s12155-025-10834-y","DOIUrl":"10.1007/s12155-025-10834-y","url":null,"abstract":"<div><p>The avocado seed is a major waste generated by the avocado agroindustry in different countries. This waste can be valorized by producing biofuels and other products through a circular economy approach. In this work, the Kissinger–Akahira–Sunose (KAS) and Ozawa-Flynn Wall (OFW) isoconversional methods were used to estimate the activation energy of pyrolysis, which ranged from 24.17 to 226.34 kJ/mol. Additionally, avocado seeds were pyrolyzed in laboratory equipment to generate biochar, gas, and bio-oil. Bio-oil was obtained with a yield of 4% and a maximum caloric value of 21.641 kJ/kg, with a high hydrocarbon content. Biochar (37.5 wt.%) was also obtained, which gained energy and activated carbon. Gas is significant in the pyrolysis process, with 60% productivity. Three cases of pyrolysis processes were simulated, which presented better experimental performance related to biochar and bio-oil yields. These cases were assessed via a sensitivity analysis of the economic component, where the economic margin of both pyrolytic products is positive for any sale price. In the minimum price of biochar, the economic margin was approximately 2%, whereas in the maximum price, the profit was approximately 60%. For the case of bio-oil, the maximum profit was approximately 35%. This research demonstrated the high potential of rapid pyrolysis of avocado seed residues for obtaining bio-oil and biochar fuels with high energy values and promoting a circular economy.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10834-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Assessment of Carbon Reduction Potential in Regional Agricultural Systems Based on Bioenergy: A Case Study of Sichuan Province, China","authors":"Kailai Ji,&nbsp;Jian Pei,&nbsp;Wenfeng Huang","doi":"10.1007/s12155-025-10833-z","DOIUrl":"10.1007/s12155-025-10833-z","url":null,"abstract":"<div><p>Currently, China's agricultural sector is undergoing a significant transition towards sustainable development, with increasing attention being paid to the environmental impacts of carbon emissions from agricultural production processes. In response to global climate change and growing energy demands, bioenergy has emerged as a potential solution; however, its specific impacts on regional agricultural systems require further clarification. This study employs a comprehensive data modeling approach to comparatively analyze the greenhouse gas emission reduction and energy conversion potentials of two prominent bioenergy technologies—anaerobic digestion and pyrolysis—within the context of agricultural biomass utilization. Furthermore, we conduct a life cycle assessment of bioenergy technology's carbon emission reduction benefits in Sichuan Province's agricultural production system. The key findings are as follows: (1) Both biomass energy conversion technologies demonstrate significant emission reduction capabilities, with anaerobic digestion technology showing a carbon reduction potential of 8.189 million tons, compared to pyrolysis technology's more substantial potential of 15.249 million tons. (2) The implementation of bioenergy technologies could potentially reduce carbon emissions by approximately 799,000 tons (-22%), 10.209 million tons (-37.0%), and 1.767 million tons (-100%) in the upstream, intermediate, and downstream sectors of Sichuan's agricultural system, respectively. (3) Under optimized scenarios involving enhanced agricultural residue utilization rates and improved technical efficiency, biomass energy could generate approximately 32.49 billion kWh of electricity for Sichuan Province, potentially surpassing wind and solar power to become the region's third-largest power generation source. This research provides quantitative insights into bioenergy's impacts on regional agricultural systems and evaluates the potential of agricultural biomass energy in Sichuan Province, offering valuable support for the development of regional sustainable agricultural strategies.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermochemical Quality from Biomass of Eucalyptus Species in Short Rotation Systems","authors":"Fernando Resquin,&nbsp;Leonidas Carrasco-Letelier,&nbsp;Cecilia Rachid-Casnati,&nbsp;Nestor Tancredi,&nbsp;Andres Hirigoyen","doi":"10.1007/s12155-025-10830-2","DOIUrl":"10.1007/s12155-025-10830-2","url":null,"abstract":"<div><p>Using biomass of forest origin to obtain solid, liquid, and gaseous fuels has demonstrated its potential both at an experimental level and in commercial situations. This framework evaluated <i>Eucalyptus benthamii</i>, <i>E. dunnii</i>, and <i>E. grandis</i> planted at densities of 2220, 3330, 4440, and 6660 trees per hectare 56 months after planting. The chemical composition and heating value of biomass fractions were evaluated. The <i>LHV</i> value (KJ/Kg) of the bark was 17,227, 16,813, and 16,167; the leaves 22,491, 23,031, and 21,358; the wood 18,725, 18,577, and 18,828 for <i>E. benthamii</i>, <i>E. dunnii</i>, and <i>E. grandis</i>, respectively. The <i>VM</i>, <i>FC</i>, and <i>A</i> contents (%) were 82, 12, and 6; 84, 11, and 5; and 89, 10, and 1 for <i>E. benthamii</i>; 84, 9, and 7; 84, 10, and 6; and 90, 9, and 1 for <i>E. dunnii</i>; and 84, 8, and 7; 84, 11, and 5; and 90, 9, and 1 for <i>E. grandis</i> in the bark, leaves, and wood fractions, respectively. The contents of <i>C</i>, <i>H</i>, and <i>O</i> (%) were 48, 5, and 40; 57, 6, and 30; and 50, 5, and 44 for <i>E. benthamii</i>; 47, 5, and 41; 57, 6, and 28; and 50, 5, and 44 for <i>E. dunnii</i>; and 46, 5, and 42; 56, 6, and 32; and 51, 5, and 44 for <i>E. grandis</i> in the bark, leaves, and wood fractions, respectively. <i>E. grandis</i> is the species that combines the best results from the point of view of its energy potential at the same time as an absence of effects due to planting density. The prediction models for the heating value were based on the carbon content.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Wood Ash, Lime, and Biochar on the Establishment and Early Growth of Poplars on Acidic Soil Conditions","authors":"Luca Muraro,&nbsp;Anneli Adler,&nbsp;Henrik Böhlenius","doi":"10.1007/s12155-025-10831-1","DOIUrl":"10.1007/s12155-025-10831-1","url":null,"abstract":"<div><p>Poplars are traditionally cultivated on arable land, but other land types, such as forested land and forested arable land, may also provide significant opportunities for poplar plantations without competing with food production. However, these sites often have suboptimal soil pH levels that hinder optimal poplar growth, highlighting the need for improved establishment methods to enhance both survival and growth. This study investigates the establishment and growth of poplars (<i>Populus trichocarpa</i> and their hybrids) at forest land and forested arable land after application of wood ash, lime, and biochar using three different application methods: (i) amendment spread on the soil (Surface), (ii) amendment mixed with the soil (Mixed), (iii) amendment placed on the planting spot (Spot). Our findings revealed that wood ash and lime application almost double growth compared to untreated plants, 3 years after planting, and that growth increased equally independently whether wood ash or lime was mixed with the soil or applied on the soil surface while Spot application method resulted in overall lower growth than the Mix and Surface method. In contrast, biochar application had a lower effect on tree growth compared to wood ash and lime. This study highlights the potential of using wood ash to improve poplar growth on sites with low soil pH and that application methods can be adapted for different site conditions, thereby supporting the early establishment of these fast-growing plantations in sites with suboptimal soil conditions.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10831-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixotrophic Cultivation of Polar Microalga Chlamydomonas sp. RCC2488 (Malina) Using Potato Peel Hydrolysates as Carbon Source","authors":"Shah Rucksana Akhter Urme,&nbsp;Laura Barth,&nbsp;Leobardo Serrano-Carreón,&nbsp;Alfredo Martinez,&nbsp;Daniela Morales-Sánchez","doi":"10.1007/s12155-025-10829-9","DOIUrl":"10.1007/s12155-025-10829-9","url":null,"abstract":"<div><p>The polar microalga <i>Chlamydomonas</i> sp. RCC2488 (malina) exhibits high PUFA production under phototrophic conditions, but there is no evidence of heterotrophic or mixotrophic growth. Hence, the grow and transmembrane carbon (glucose) transport was assessed under heterotrophic and mixotrophic conditions. The microalga transported carbon at a rate of 0.015 μmol/g/min, achieving 0.4 g/L of biomass with high PUFA content (122.5 mg/g) under mixotrophic conditions with glucose. No heterotrophic growth and transport were observed. The use of an alternative carbon source such as potato peel waste was evaluated. This material was subjected to an acid-hydrothermal pretreatment with 1% sulfuric acid at 121 °C for 120 min followed by enzymatic hydrolysis with α-amylase and amyloglucosidase. The resulting hydrolysates (PPH1 and PPH3) released up to 46.3 g/L of glucose with a reducing sugar conversion of 46%. But these hydrolysates also presented up to 4.7 and 8.5 g/L of furfural and hydroxymethylfurfural (HMF), respectively, which decreased the microalgal growth to 0.07 g/L and promoted the accumulation of 74.7 g/g of TAG. A water-washing extra step (PPH2) removed these toxic compounds achieving biomass and PUFA concentrations similar to the ones obtained with pure glucose. Therefore, pretreatment and hydrolysis optimization including toxic by-product removal are necessary to use potato peel as an efficient carbon source for microalgae cultivation. Mixotrophic cultivation of <i>Chlamydomonas</i> sp. RCC2488 (malina) was possible but not ideal since higher growth is attained under phototrophic conditions.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10829-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Neoscytalidium dimidiatum ITD-G6 from Agave durangensis Leaves for Enhanced Cellulase Production in Solid-State Cultivation","authors":"M. G. Contreras-Hernández,&nbsp;O. M. Rutiaga-Quiñones,&nbsp;F. J. Mares-Rodríguez,&nbsp;J. R. Irigoyen-Campuzano,&nbsp;I. N. Cordero-Soto,&nbsp;E. T. Aréchiga-Carvajal","doi":"10.1007/s12155-025-10827-x","DOIUrl":"10.1007/s12155-025-10827-x","url":null,"abstract":"<div><p>The cellulase production strategy considers the selection of microorganisms and the best experimental conditions for fermentation. This work describes the molecular identification of a fungus isolated from <i>Agave durangensis</i>, employing the internal transcribed spacer 18 S rRNA gene, located in the small subunit 28 S rRNA gene, belonging to a larger subunit, elongation factor 1-α, and the β-tubulin sequences. The best experimental conditions for improving cellulase production using <i>A. durangensis</i> leaves as a solid-state fermentation substrate were also evaluated. A profile of enzymatic activity of endoglucanase (Endo-β), exoglucanase (Exo-β), and β-glucosidase (β-g) obtained at 96 h is presented. Sequences used in the identification analysis of the isolated fungus identified it as a phytopathogen species of <i>Neoscytalidium dimidiatum</i> ITD-G6, belonging to the Botryosphaeriaceae family. To the authors’ awareness, this is the first report where leaves of <i>A. durangensis</i> are hosts of this microorganism. The maximum enzymatic activities were obtained at 72 h (2.78 ± 0.015 IU/mL) and 96 h (3.44 ± 0.017 IU/mL), under 37 °C, pH = 4.5, and 70% humidity. At these conditions, the cellulolytic activity of the three enzymes was as follows: Endo-β (0.0547 ± 0.0167 IU/mL), Exo-β (0.0163 ± 0.001 IU/mL), and β-g (0.0692 ± 0.07 IU/mL). The data presented suggest that the isolated fungus has biotechnological potential as a source of cellulases, contributing to the field of little-studied phytopathogens, owing to the feasibility of biomass conversion.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Fertilization, Soil Texture, and Forking on the Wood Quality and Yield of Energy Plantations of Tachigali vulgaris in Amazonia","authors":"Elvis Vieira dos Santos,&nbsp;Álvaro Augusto Vieira Soares,&nbsp;Flávio Henrique Silveira Rabêlo,&nbsp;Sérgio Henrique Godinho Silva,&nbsp;Michael Douglas Roque Lima,&nbsp;Lina Bufalino,&nbsp;Jonathan Dias Marques,&nbsp;Delman de Almeida Gonçalves,&nbsp;Arystides Resende Silva,&nbsp;Edgar A. Silveira,&nbsp;Paulo Fernando Trugilho,&nbsp;Thiago de Paula Protásio","doi":"10.1007/s12155-025-10828-w","DOIUrl":"10.1007/s12155-025-10828-w","url":null,"abstract":"<div><p>Biomass from planted forests is a renewable energy source with the potential to replace fossil fuels. <i>Tachigali vulgaris</i> wood shows potential for energy applications, but studies on fertilization and stem-type effects remain limited. This study evaluated the influence of stem type, soil texture, and fertilization on the energy potential and wood quality of <i>T. vulgaris</i>. The research was conducted in two experimental plantations in Pará, Brazil, on sandy loam and very clayey soils, with 11-year-old trees in four blocks per area. Three fertilization treatments with phosphorus and potassium were applied. Wood basic density (WBD), proximate analysis, heating value, extractive content, lignin content, wood dry mass, lignin mass, and wood energy productivity (EP) were measured. Fertilization treatments did not significantly affect the WBD, with a 0.514 g/cm³ average. However, every 2 cm increment in equivalent diameter (EqD) corresponded to a 0.01 g/cm³ rise in WBD. Trees with an EqD below 15 cm had WBD below 0.500 g/cm³. Higher fixed carbon (16.31% on a dry mass basis) was observed in very clayey soils. The treatments and their interactions significantly influenced extractives (4.65% dry basis), insoluble lignin (29.43% extractive-free dry basis, efdb), and total lignin (31.34% efdb). EP varied between 1739.23 and 2760.07 GJ/ha, with higher values for larger EqD. Growth parameters, including EqD, significantly influenced EP, with a 2.65 GJ/tree average. These findings highlight the importance of growth characteristics in evaluating species for energy forestry.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s12155-025-10828-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Cellulase From Mutated Aspergillus sp. for the Production of Sustainable 2G Ethanol From Sugarcane Bagasse","authors":"Satwika Das,&nbsp;Dharmendra Shakya,&nbsp;Ramandeep Kaur,&nbsp;Naveen Kumar B,&nbsp;Chandukishore T,&nbsp;Surajbhan Sevda,&nbsp;Rangabhashiyam Selvasembian,&nbsp;Ashish A. Prabhu","doi":"10.1007/s12155-025-10825-z","DOIUrl":"10.1007/s12155-025-10825-z","url":null,"abstract":"<div><p>Sugarcane bagasse (SB) is a lucrative feedstock for sustainable fuel production, but economical conversion into fermentable sugars and ethanol presents challenges from a biorefinery perspective. The present study aimed to screen and identify a robust cellulase-producing fungal strain to improve the saccharification of SB. <i>Aspergillus fumigatus</i> exhibited enhanced cellulase activity when untreated SB served as the substrate. The EMS mediated chemical mutagenesis of <i>A. fumigatus</i> with 200 mM EMS further enhanced the cellulase production by approximately 23.47%, relative to the wild-type strain. The process optimization method demonstrated peak cellulase activity on the 6th day of incubation, at 33 °C, and with an inoculum size of 5 × 10⁷ spores. The optimization of the filter paper assay enhanced the maximum activity to 2.5 U/mL by maintaining 6 pH and 55 °C, along with the subsequent addition of MnCl₂ ions to the reaction mixture. The Taguchi orthogonal array was employed to optimize the process parameters of enzymatic hydrolysis of alkali-pretreated SB, demonstrating highest efficiency when hydrolysis parameters were set to pH 4, 55 °C, 10 U enzyme, and 20 g/L substrate (SB) loading. The hydrolysate was utilized to evaluate bioethanol production employing <i>Saccharomyces cerevisiae</i> MTCC 824. The strain generated 4.2 g/L of ethanol with a total yield of 0.21 g/g. This study seeks to manage agricultural residues and wastes, generating a nutrient-rich hydrolysate that can be utilized by yeast strains for bioethanol production, thus rendering the entire process sustainable, environmentally friendly, and cost-effective.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}