Hydrogen Bonding Improvement of Urea Assisting Potassium Hydroxide Pretreatment to Enhance Enzymatic Hydrolysis of Rice Straw

IF 3.1 3区工程技术 Q3 ENERGY & FUELS

BioEnergy Research Pub Date : 2025-05-12 DOI:10.1007/s12155-025-10845-9

Xiangliang Du, Wen Wang, Cuiyi Liang, Shiyou Xing, Yu Zhang, Wei Liu, Xuesong Tan, Wei Qi

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引用次数: 0

Abstract

KOH-urea pretreatment of lignocellulose has increasingly gained attention due to its mild reaction condition and recyclable liquid waste. However, the mechanism of urea assisting KOH pretreatment to improve enzymatic hydrolysis of lignocellulose remains to be further explored. This study found that urea addition could enhance KOH solution to remove lignin from rice straw (RS). When the ratio of KOH to urea was 1:1, the treated RS which was composed of 44.58 ± 0.39% glucan, 15.14 ± 0.12% xylan, and 12.40 ± 0.06% acid-insoluble lignin attained the maximum total sugar production of 57.52 ± 2.42 mg/mL and enzymatic hydrolysis efficiency of 84.34 ± 4.85%. The structural features of KOH and KOH-urea-treated RS samples were characterized by scanning electron microscopy (SEM), Brunauer-Emmet-Teller (BET), Fourier transform infrared spectrometer (FTIR), and X-ray diffraction (XRD). It showed that a proper urea addition could intensify the physical structure changes and chemical groups release of KOH-treated RS. After detecting the hydrogen bonds in KOH and KOH-urea solutions, it was found that an appropriate urea addition could improve the hydrogen bonding linkage between chemical reagents and lignin. It might be the reason for maximum enzymatic hydrolysis of KOH-urea-treated lignocellulose which was obtained with a suitable addition of urea. This study provided some evidences to explain the mechanism of urea facilitating KOH pretreatment of lignocellulose.

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改善尿素氢键助氢氧化钾预处理促进稻草酶解的研究

koh -尿素预处理木质纤维素因其反应条件温和、废液可循环利用而日益受到重视。然而，尿素辅助KOH预处理改善木质纤维素酶解的机理还有待进一步探讨。本研究发现尿素能增强KOH溶液对稻草木质素的脱除效果。当KOH与尿素的比例为1:1时，由44.58±0.39%葡聚糖、15.14±0.12%木聚糖和12.40±0.06%酸不溶性木质素组成的RS的最大总糖产量为57.52±2.42 mg/mL，酶解效率为84.34±4.85%。采用扫描电镜（SEM）、布鲁诺尔-埃米特-泰勒（BET）、傅里叶变换红外光谱仪（FTIR）和x射线衍射仪（XRD）对KOH和KOH-尿素处理的RS样品的结构特征进行了表征。结果表明，适当的尿素添加量可以增强KOH和KOH-尿素溶液中木质素的物理结构变化和化学基团的释放，通过检测KOH和KOH-尿素溶液中的氢键，发现适当的尿素添加量可以改善化学试剂与木质素之间的氢键连接。这可能是在适当的尿素添加量下，koh -尿素处理的木质纤维素酶解效果最好的原因。本研究为尿素促进木质纤维素KOH预处理的机理提供了一些依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BioEnergy Research ENERGY & FUELS-ENVIRONMENTAL SCIENCES

CiteScore

6.70

自引率

8.30%

发文量

174

审稿时长

3 months

期刊介绍： BioEnergy Research fills a void in the rapidly growing area of feedstock biology research related to biomass, biofuels, and bioenergy. The journal publishes a wide range of articles, including peer-reviewed scientific research, reviews, perspectives and commentary, industry news, and government policy updates. Its coverage brings together a uniquely broad combination of disciplines with a common focus on feedstock biology and science, related to biomass, biofeedstock, and bioenergy production.