Bioresource Technology最新文献

Temperature-dependent mechanisms of volatile sulfur compound transformation and emission during mesophilic and thermophilic anaerobic digestion of sludge. 污泥中温和嗜热厌氧消化过程中挥发性硫化合物转化和排放的温度依赖机制。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-09-08 DOI: 10.1016/j.biortech.2026.135823

Xiang Li, Bowen Wang, Shengwei Wang, Silan Zhao, Jiayi Yuan, Minghui Dang, Yan Zhao

{"title":"Temperature-dependent mechanisms of volatile sulfur compound transformation and emission during mesophilic and thermophilic anaerobic digestion of sludge.","authors":"Xiang Li, Bowen Wang, Shengwei Wang, Silan Zhao, Jiayi Yuan, Minghui Dang, Yan Zhao","doi":"10.1016/j.biortech.2026.135823","DOIUrl":"10.1016/j.biortech.2026.135823","url":null,"abstract":"Volatile sulfur compounds (VSCs) are key odorants in sludge anaerobic digestion (AD), but their temperature-dependent transformation mechanisms remain unclear. This study compared mesophilic (35 °C) and thermophilic (55 °C) AD by integrating VSC profiles, microbial communities, sulfur-related enzymes, methionine degradation, and EPS characteristics. Mesophilic digestion produced 24.25 μg·g-1 TS VSCs, with H2S accounting for 96.91%, whereas MM, DMS, and DMDS were marginal. Thermophilic digestion increased total VSCs to 180.27 μg·g-1 TS and shifted dominance to MM (61.04%), followed by H2S (29.87%), DMDS (7.79%), and DMS (1.30%). Methionine degradation increased from 37.55% to 67.24%. Putative sulfur-oxidizing bacteria reached 15.1% and 20.9% under mesophilic and thermophilic conditions, respectively, with Arcobacter and Acinetobacter as dominant genera, while Caldisericum emerged after day 6 at 55 °C. Thermophilic digestion increased indicators associated with APS kinase, sulfate reductase, thioredoxin reductase, thiol peroxidase, and sulfide quinone oxidoreductase by 80.49%, 166.55%, 137.47%, 151.84%, and 85.81%, respectively. It also altered LB-EPS and TB-EPS fluorescence and increased protein-like, fulvic-acid-like, and microbial byproduct-like components. Overall, temperature acted as a metabolic switch, redirecting sulfur transformation from H2S-dominated emissions toward MM/DMDS-dominated organic sulfur volatilization through coordinated microbial, enzymatic, and EPS regulation.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135823"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Elucidating the aromatic formation of humus via quorum sensing and redox function of thermophilic microbiota in compost" [Biores. Technol. 464 (2027) 135739]. “通过群体感应和堆肥中嗜热微生物群的氧化还原功能阐明腐殖质的芳香形成”的勘误表[Biores]。技术通报。464(2027)135739]。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-09-29 DOI: 10.1016/j.biortech.2026.135948

Xiaolin Lu, Qiqiang Zhang, Yaling Peng, Zeying Liang, Yuting Peng, Xiaorui Zhang, Guixian Liu, Guangsheng Qin, Qunliang Li

引用次数: 0

Corrigendum to "Biochar promotes the biophotoelectrochemical process driven by chlorophyll a and electroactive microorganisms" [Bioresour. Technol. 461 (2026) 135494]. “生物炭促进由叶绿素a和电活性微生物驱动的生物光电化学过程”的勘误表[Bioresour]。[j].科学通报，2016,32(5):354 - 354。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-10-13 DOI: 10.1016/j.biortech.2026.136029

Yuting Yang, Jingqi Zheng, Shuyi Xie, Shanshan Chen, Tiangang Luan

引用次数: 0

Unlocking the energy potential of orange wastes using various pre-treatments and co-digestion strategy: Technological and energy analysis and polycyclic aromatic hydrocarbons distribution. 利用各种预处理和共消化策略释放橙子废弃物的能源潜力：技术和能源分析以及多环芳烃分布。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-08-15 DOI: 10.1016/j.biortech.2026.135633

Aleksandra Szaja, Agnieszka Montusiewicz, Sylwia Pasieczna-Patkowska, Izabela Bartkowska, Magdalena Lebiocka, Paweł Golianek, Iwona Musielewicz

{"title":"Unlocking the energy potential of orange wastes using various pre-treatments and co-digestion strategy: Technological and energy analysis and polycyclic aromatic hydrocarbons distribution.","authors":"Aleksandra Szaja, Agnieszka Montusiewicz, Sylwia Pasieczna-Patkowska, Izabela Bartkowska, Magdalena Lebiocka, Paweł Golianek, Iwona Musielewicz","doi":"10.1016/j.biortech.2026.135633","DOIUrl":"10.1016/j.biortech.2026.135633","url":null,"abstract":"This study investigated the impact of using orange waste (OW) pre-treated by various methods on anaerobic co-digestion (AcoD) with sewage sludge (MSS). The analyses concerned the methane production and its kinetics, organic matter degradation, including polycyclic aromatic hydrocarbons (PAHs), as well as energy balance assessment. Moreover, the study employed the Fourier transform infrared spectroscopy - attenuated total reflectance and thermogravimetric and derivative thermogravimetry analyses to describe the transformation occurring within the anaerobic digestion process (AD). The following methods were chosen: both acoustics (AC) and hydrodynamic cavitation (HC), microwave irradiation (MR), and impact of solidified carbon dioxide (SCD). The AD experiments were conducted under mesophilic conditions in a batch mode. Two reference trials were provided, i.e., the mono-digestion of MSS (A0) and the co-digestion of MSS and untreated OW (A1). In other trials, the co-digestion of MSS and pre-treated OW was performed (A2-A5). The most beneficial results were achieved in the case of the OW pre-treated using HC. Therein, improvements of 7.5 and 15.3% in methane production were found compared to A0 and A1, respectively. Importantly, the results of energy balance showed that for HC, the energy profit was 54 and 43%, compared to A0 and A1, respectively. This finding was also supported by improved organic removal efficiency and effective destruction of 3-, 5-, and 6-ring PAHs, which indicated a high performance of the AcoD process.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135633"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rumen solids as a superior inoculum for in vitro volatile fatty acid production: Effects and metagenomic insights. 宏基因组学显示，瘤胃残留物是体外生产挥发性脂肪酸的优良接种物。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-08-14 DOI: 10.1016/j.biortech.2026.135627

Yuehan Li, Le Chen, Jiasheng Zhang, Yajie Zhang, Maoqing Wang, Ru Zhang, Wei Fang, Panyue Zhang, Guangming Zhang

{"title":"Rumen solids as a superior inoculum for in vitro volatile fatty acid production: Effects and metagenomic insights.","authors":"Yuehan Li, Le Chen, Jiasheng Zhang, Yajie Zhang, Maoqing Wang, Ru Zhang, Wei Fang, Panyue Zhang, Guangming Zhang","doi":"10.1016/j.biortech.2026.135627","DOIUrl":"10.1016/j.biortech.2026.135627","url":null,"abstract":"Rumen microbiome is widely recognized as an efficient system for lignocellulose degradation. Rumen fluid (RF) has been often used as the inoculum in previous study of bioprocesses, however, the potential of rumen solids (RS) to enhance volatile fatty acid (VFA) production remains underexplored. The anaerobic fermentation performance of RS, RF, and RF + RS mixture as inocula was compared using corn stover as substrate, with RS addition at 5%, 10%, or 20% (w/w) to corn stover and RF addition at 1:2 (v/v) to buffer solution. At a corn stover content of 2.5% (w/v, based on final working volume), the highest VFA concentration of 10.05 g/L was achieved with 20% RS as inoculum, outperforming those with both RF and RF + RS. Metagenomic analysis revealed significant differences in bacterial, fungal, and archaeal community structures with 20% RS and RF. With 20% RS, hydrolytic bacteria (e.g., Enterobacter) dominated the ecological niche, microbial co-occurrence network analysis of the 2,000 most abundant genera revealed a simpler network with fewer negative associations, and functional analysis demonstrated a notable increase in relative abundance of glycosyltransferase (GT) families within carbohydrate-active enzymes (CAZymes). With 20% RS, enrichment in glycolysis-related genes was observed, indicating a preference for carbohydrate degradation, while acid-producing pathways were enriched such as pyruvate metabolism with RF. Rumen solids, as a superior inoculum for VFA production, effectively enhanced lignocellulose bioconversion by enriching specific low-abundance microbial taxa, forming a microbial network with fewer negative associations, increasing the relative abundance of GT families, and strengthening the systemic hydrolysis capacity.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135627"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-function relationships between residence time distribution and lignocellulosic deconstruction and fermentation yield of wheat straw during reactive extrusion pretreatment. 反应挤压预处理过程中停留时间分布与麦秸木质纤维素分解及发酵产率的结构-功能关系

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-08-15 DOI: 10.1016/j.biortech.2026.135610

Huimin Zhang, Jiantao Guo, Xiaodong Xu, Suan Shi, Xian Liu, Lujia Han

{"title":"Structure-function relationships between residence time distribution and lignocellulosic deconstruction and fermentation yield of wheat straw during reactive extrusion pretreatment.","authors":"Huimin Zhang, Jiantao Guo, Xiaodong Xu, Suan Shi, Xian Liu, Lujia Han","doi":"10.1016/j.biortech.2026.135610","DOIUrl":"10.1016/j.biortech.2026.135610","url":null,"abstract":"Reactive extrusion is an effective pretreatment method for straw. However, the structure-function relationships between residence time distribution (RTD) in reactive extrusion and lignocellulosic deconstruction and fermentation yield of wheat straw remain unclear. Therefore, the RTD during reactive extrusion of wheat straw was calculated using the pulse method, and its physicochemical properties before and after pretreatment, as well as the yields obtained from microbial-enzymatic co-fermentation, were determined to establish the structure-function relationship between the two. The results showed that RTD varied significantly under different operating conditions. After pretreatment, lignocellulose was effectively deconstructed, with lignin removal ranging from 13.04% to 21.71%. The total sugar yield and lactic acid increased to 2.27-2.95 times and 1.50-1.61 times those of untreated samples, respectively. The structure-function relationships indicated that an appropriate mean residence time and sufficient mixing during extrusion are beneficial for lignocellulosic deconstruction, thereby enhancing enzymatic hydrolysis and fermentation performance of wheat straw. This study provides a theoretical basis for the sustainable fractionation and efficient utilization of lignocellulosic biomass via reactive extrusion.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135610"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomass-derived carbon porous frameworks: from formation mechanisms to sustainable functional materials. 生物质衍生的碳多孔框架：从形成机制到可持续功能材料。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-08-16 DOI: 10.1016/j.biortech.2026.135641

Aleesha Nabhai, Zhengbai Li, Ilma Sang Mainardi, Laura Hernandez Rodriguez, Chandler Ray Sheets, Jinghao Li

{"title":"Biomass-derived carbon porous frameworks: from formation mechanisms to sustainable functional materials.","authors":"Aleesha Nabhai, Zhengbai Li, Ilma Sang Mainardi, Laura Hernandez Rodriguez, Chandler Ray Sheets, Jinghao Li","doi":"10.1016/j.biortech.2026.135641","DOIUrl":"10.1016/j.biortech.2026.135641","url":null,"abstract":"Carbon porous frameworks (CPFs) are lightweight, mechanically robust materials with hierarchical porosity and tunable surface chemistry. However, conventional CPFs are predominantly produced from petroleum-derived precursors, limiting long-term sustainability. Biomass-derived carbon porous frameworks (BCPFs) offer a renewable and chemically rich alternative. Their inherent heteroatom content and oxygen-containing functional groups enable multifunctional porous frameworks through thermally driven pathways. Despite rapid progress, the fundamental relationships between biomass chemistry, thermal conversion mechanisms, and hierarchical framework formation remain poorly understood. Existing reviews primarily emphasize precursor selection or application performance, with limited discussion of the mechanisms governing structure evolution and property development. This review critically establishes mechanistic correlations between precursor chemistry, thermal conversion pathways, and resulting hierarchical porous frameworks. The distinct thermal decomposition behaviors of lignin, cellulose, and hemicellulose are discussed in relation to foamability, shrinkage, and framework stability. The major BCPF fabrication strategies are critically compared, and mechanism-guided design principles are proposed to advance scalable and sustainable BCPF development for energy storage, environmental remediation, and gas capture applications.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135641"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Apparent partial denitrification and anammox contribution to nitrogen removal in full-scale multistage anoxic-oxic system treating food waste digestate" [Biores. Tech. 464 (2027) 135951]. “明显的部分反硝化和厌氧氨氧化对全规模多级厌氧-厌氧系统处理食物垃圾消化液中氮去除的贡献”的更正[Biores]。科技。464(2027)135951]。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-10-17 DOI: 10.1016/j.biortech.2026.136061

Wen-Bo Liang, Ming-Rui Shao, Xi-Hui Fan, Dai-Xin Liu, Zhi-Shui Liang, Xiao-Li Yang

引用次数: 0

Raman spectroscopic quantification of phycocyanin and process metadata-based data fusion for dry cell weight in Spirulina platensis cultivation. 藻蓝蛋白的拉曼光谱定量及基于过程元数据的数据融合测定螺旋藻培养中干细胞重。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2027-01-01 Epub Date: 2026-08-25 DOI: 10.1016/j.biortech.2026.135728

Xiaoxuan Zhou, Xuan Wang, Shuo Wei, Zhongliang Sun, Liwei Hu, Dandan Zhao, Jianxiong Hao, Xuechao Zheng

{"title":"Raman spectroscopic quantification of phycocyanin and process metadata-based data fusion for dry cell weight in Spirulina platensis cultivation.","authors":"Xiaoxuan Zhou, Xuan Wang, Shuo Wei, Zhongliang Sun, Liwei Hu, Dandan Zhao, Jianxiong Hao, Xuechao Zheng","doi":"10.1016/j.biortech.2026.135728","DOIUrl":"10.1016/j.biortech.2026.135728","url":null,"abstract":"Rapid and non-destructive quantification of intracellular target products is crucial for rapid at-line process control in modern microalgae biorefineries. This study established a small-sample (50 independent biological samples) quantitative monitoring framework based on Raman spectra for live Spirulina platensis cells. For intracellular phycocyanin (PC) content quantification, a linear combination of raw full-range spectra, regression coefficients (RC) feature selection, and partial least squares regression (PLSR) delivered the optimal performance, achieving a prediction coefficient of determination (R2) of 0.944 and a relative prediction deviation (RPD) of 4.22. To further extend this monitoring framework to the macro-biomass indicator, dry cell weight (DCW) quantification was simultaneously evaluated. Pure Raman spectral signals, however, encountered non-linear matrix interferences for DCW (RPD < 2.00). To overcome this bottleneck, a multi-source information fusion strategy combining process metadata (strain, light, and cultivation time) side-by-side with optimized spectral features into a fused input matrix was developed. The resulting non-linear Savitzky-Golay (SG) smoothing, successive projections algorithm (SPA) feature selection, and support vector regression (SVR) framework significantly improved the R2 and RPD for DCW to 0.937 and 3.98, respectively. Furthermore, Shapley Additive exPlanations (SHAP) analysis successfully demystified the model's black-box nature, revealing that cultivation time exerted a dominant contribution (mean absolute SHAP value = 0.855) to DCW accumulation. This strategy reduces the reliance on massive databases, offering a highly transparent, low-sample-cost, and lightweight novel solution for implementing intelligent microalgae process analytical technology.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135728"},"PeriodicalIF":8.2,"publicationDate":"2027-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vesicle Nucleating peptide 6 (VNp6) mediates efficient soluble expression of recombinant proteins in Escherichia coli. 囊泡成核肽6 （VNp6）介导重组蛋白在大肠杆菌中的高效可溶性表达。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-12-01 Epub Date: 2026-08-15 DOI: 10.1016/j.biortech.2026.135615

Jingxin Luo, Binbin Wang, Guifang Tian, Shiyu Sun, Qingran Shen, Baomei Wu, Xinxiang Zhang, Yaxin Sang, Fangkun Zhao

{"title":"Vesicle Nucleating peptide 6 (VNp6) mediates efficient soluble expression of recombinant proteins in Escherichia coli.","authors":"Jingxin Luo, Binbin Wang, Guifang Tian, Shiyu Sun, Qingran Shen, Baomei Wu, Xinxiang Zhang, Yaxin Sang, Fangkun Zhao","doi":"10.1016/j.biortech.2026.135615","DOIUrl":"10.1016/j.biortech.2026.135615","url":null,"abstract":"Escherichia coli is a dominant host for recombinant protein production due to its well-characterized genetics and low cultivation cost, yet high-level intracellular expression frequently leads to insoluble inclusion bodies. Targeting proteins to the periplasm represents an effective strategy for improving soluble expression, but the efficiency of conventional signal peptides is highly protein-specific, restricting their universal utility. This study investigates an artificially engineered peptide, Vesicle Nucleating peptide 6 (VNp6), and systematically evaluates its capacity to mediate recombinant protein expression with green fluorescent protein (GFP) as a reporter. Results revealed that VNp6 markedly enhanced intracellular soluble GFP expression at 37 ℃, 25 ℃, and 16 ℃. Its intracellular fluorescence intensity was 128-fold and 220-fold higher than that of PelB and OmpA at 37 ℃, 82-fold and 113-fold at 25 ℃, and 107-fold and 160-fold at 16 ℃, respectively. Subcellular localization confirmed predominant periplasmic localization and improved solubility of the protein. Relative to the native periplasmic signal peptides FimH and PhoA, VNp6 improved solubility by 4‑fold and 14‑fold, respectively. For SacB, TVE, and amylase, solubility was enhanced by 27%, 30% and 17% at 16 ℃, respectively. Although extracellular protein accumulation was partially attributable to cell lysis, VNp6 consistently promoted periplasmic protein accumulation and enhanced soluble protein production across multiple recombinant proteins. Collectively, these results demonstrate that VNp6 serves as a powerful tool for improving periplasmic protein accumulation and soluble expression in E. coli expression systems.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135615"},"PeriodicalIF":8.2,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0