Bioresource Technology最新文献

{"title":"Dose-dependent effects of biochar on low-temperature anammox: reactor performance, community variation, and functional potential.","authors":"Kaiyu Hou, Biao Yang, Rong Zhao, Jinwei Zhang, Yun Duan","doi":"10.1016/j.biortech.2026.135296","DOIUrl":"10.1016/j.biortech.2026.135296","url":null,"abstract":"<p><p>Low temperature is a major constraint on the practical application of anaerobic ammonium oxidation (anammox). Although biochar has been reported to improve low-temperature anammox, the effect of dosage remains insufficiently understood. In this study, mature anammox sludge was amended with 0, 3, 7, and 9 g/L bamboo-derived biochar and operated under a stepwise temperature decrease from 35 to 15°C, followed by low-temperature operation for 70 d. Reactor performance, extracellular polymeric substances (EPS), microbial community composition, and metagenomic functional potential were analyzed to clarify the dose effect of biochar. Among the tested dosages, 7 g/L biochar achieved the highest nitrogen removal efficiency (48.6%) at 15°C, which was 12.8 percentage points higher than the control value of 35.8%. Biochar-amended reactors also showed higher EPS contents than the control, and the 7 g/L group better maintained the PN/PS ratio under low-temperature stress. Community analysis indicated a higher relative abundance of Candidatus Brocadia in the biochar-amended groups, especially at 7 g/L. Metagenomic analysis further showed higher abundance of genes associated with nitrogen metabolism, carbon metabolism, and EPS-related precursor synthesis in the 7 g/L group. These results suggest that an appropriate biochar dosage can improve low-temperature anammox performance and is associated with EPS stabilization, enrichment of key functional taxa, and enhanced functional potential. This study provides guidance for biochar dosage optimization in low-temperature anammox systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135296"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Waste salt-assisted pyrolysis of antibiotic fermentation residue for biochar production and hexavalent chromium reduction mechanism\" [Bioresou. Technol. 462 (2026) 135547].","authors":"Yuan Bai, Boxuan Wang, Xin Zhang, Zhihua Chen, Kai Jiang, Dapeng Wu","doi":"10.1016/j.biortech.2026.135598","DOIUrl":"10.1016/j.biortech.2026.135598","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135598"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CO₂ availability as process tool to enhance isobutyric acid production in methanol fermentation by Clostridium luticellarii.","authors":"Camille Petrognani, Quinten Mariën, Lander De Vos, Merlijn van Oijen, Nico Boon, Ramon Ganigué","doi":"10.1016/j.biortech.2026.135255","DOIUrl":"10.1016/j.biortech.2026.135255","url":null,"abstract":"<p><p>The bioconversion of CO₂‑derived methanol into higher‑value chemicals offers an attractive route for hybrid catalytic-biotechnological carbon capture and utilization (CCU). Clostridium luticellarii is one of the few acetogens able to produce isobutyric acid. However, operational and metabolic factors driving its production are poorly understood. This work investigates how CO₂ availability shapes the product spectrum of C. luticellarii during methylotrophic growth and assesses whether CO₂ supply can be used as a process lever to promote isobutyric acid formation. Batch experiments with varying initial bicarbonate concentrations revealed that conditions leading to CO₂ limitation (i.e., DIC depletion at ≤ 30 mM NaHCO₃) redirected carbon and electron fluxes away from acetic acid toward butyric and isobutyric acids, with the latter accounting for up to 41% of total products. This metabolic switch was not observed when CO₂ was in excess (>45 mM). High acetic acid supplementation (100 mM) triggered isobutyric acid production even while CO₂ was still available, indicating a combined regulation of dissolved inorganic carbon (DIC) and acetic acid availability. Net acetic acid consumption took place in all isobutyric acid-producing experiments. These observations were reproduced in 3-L bioreactors and further exploited through a fed‑batch strategy in which an initial acetic‑acid‑accumulating phase was followed by CO₂‑limited feeding. This approach achieved complete conversion of methanol and CO₂ and yielded an isobutyric acid titer of 2.70 ± 0.04 g·L^-1. Controlling CO₂ availability is a viable operational tool to steer C. luticellarii metabolism toward isobutyric acid production, in interaction with electron acceptor availability.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135255"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxylamine steers nitrogen metabolism toward dissimilatory nitrate reduction to ammonium by suppressing competitive denitrification.","authors":"Jianfei Xu, Xiaonong Zhang, Wen Sun, Xingxing Zhang, Peng Wu, Aijie Wang","doi":"10.1016/j.biortech.2026.135307","DOIUrl":"10.1016/j.biortech.2026.135307","url":null,"abstract":"<p><p>Dissimilatory nitrate reduction to ammonium (DNRA) is important for nitrogen conservation and resource recovery in wastewater treatment, but its efficiency is often limited by competition for electrons and substrates from denitrifiers. Although hydroxylamine (NH₂OH) has been shown to modulate various nitrogen transformation processes, its long-term effects on DNRA systems and the underlying microbial ecological responses remain unclear. In this study, the nitrogen transformation performance, electron transfer characteristics, and microbial community succession in DNRA systems were comprehensively investigated under prolonged exposure to 0-5 mg/L NH₂OH. The results demonstrated that, with increasing NH₂OH concentrations, the system consistently achieved near-complete nitrate removal without nitrite accumulation, and the effluent NH₄⁺-N reached up to 51.5 mg/L, indicating a substantial enhancement of DNRA ammonium production. Functional activity analyses and apparent electron-equivalent balance suggested an increased contribution of DNRA to nitrate-reduction-associated electron consumption. Metagenomic analyses further showed that NH₂OH could decrease the relative abundances of denitrification-related genes, including nirS, norB, and nosZ, while increasing those of narG and the nrf gene cluster. Building upon the existing DNRA functionality, NH₂OH selectively enriched a tolerant DNRA population, exemplified by Ignavibacteriota, and facilitated cross-feeding interactions and electron transfer network remodeling involving fermentative bacteria. Collectively, these findings suggest that NH₂OH can weaken denitrification competition and increase the apparent contribution of DNRA to nitrate-reduction-associated electron consumption, thereby enhancing ammonium production. Moreover, these findings may provide a theoretical basis for the future development of DNRA-Anammox coupled processes for high-level nitrogen removal.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135307"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148381190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignin-polyphenol epoxy layer: a multi-functional protective coating cascade-constructed by ionic liquids.","authors":"Cheng Li, Yuting Shi, Wenzhe Xiao, Xiaoning Wang, Wanting Zhao, Wenjin Li, Shangru Zhai, Jian Sun","doi":"10.1016/j.biortech.2026.135216","DOIUrl":"10.1016/j.biortech.2026.135216","url":null,"abstract":"<p><p>Industrial lignin-derived polyphenols are promising platform chemicals, especially as bisphenol A substitutes for bio-resin production. Nevertheless, there is an urgent need to address the issues caused by traditional volatile and toxic epoxy curing agents. Herein, a novel lignin-polyphenol epoxy layer with multi-functional protective performances was successfully constructed through cascade reactions by ionic liquids (ILs). Specifically, lignin polyphenol obtained from demethylation of alkali lignin in ethanolamine acetate was cross-combined with salicylol (SA) and epichlorohydrin to synthesize lignin-based epoxy prepolymers by tetrabutylammonium bromide. And the epoxy layer was eventually obtained by solidification with dicarboxylic acid based PILs, [EOA]₂[Asp]. The resin layer has demonstrated excellent anti-corrosion, anti-ultraviolet and anti-bacterial properties. The iron sheet coated with resin layer (contact angle = 103.23°) exhibited corrosion resistance more than 2  months without any damage. A thin resin layer (0.5  mm) can achieve both excellent UV absorption properties (≥0.66 a.u.) and high UV blocking rate (96%). The positive Zeta surface potential of the resin layer is conducive to the adsorption of bacteria contributing additional antibacterial properties. Compared to lignin, lignin polyphenols can create more epoxy-binding sites and thus be cross-linked (ρ = 60.33 × 10^-3 mol/cm³) and cured with high density by more dicarboxylic acid based PILs. Meantime, the anions of the dicarboxylic acid based PILs contain multiple carboxyl groups, which can form additional dynamic physical crosslinking with the pre-polymer. This study explores new ways to utilize industrial lignin and develop ILs as green solvents for lignin-based processes.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135216"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to \"Cost-effective wireless system for image-based microalgae biomass estimation with adaptive background correction\" [Bioresour. Technol. 459 (2026) 135189].","authors":"Hua Xiao, Qiaoyang Zhang, Haiyun Chen, Junjie Hao, Haodong Tang, Kai Wang, Xiao Wei Sun","doi":"10.1016/j.biortech.2026.135351","DOIUrl":"10.1016/j.biortech.2026.135351","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135351"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into superior biofilm formation with heterotrophic nitrification-aerobic denitrification bacteria under polypropylene microplastic stress.","authors":"Yan Lin, Bixiang Nie, Xilong Liu, Qian Zhang","doi":"10.1016/j.biortech.2026.135159","DOIUrl":"10.1016/j.biortech.2026.135159","url":null,"abstract":"<p><p>Microplastics may disturb microbial activity and biofilm development in biological wastewater treatment systems, yet the response of three-dimensional rotating biological contactor start-up biofilms to polypropylene microplastic stress remains unclear. This study evaluated a biofilm initiation strategy using heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria (H-3D-RBCs) and compared it with activated sludge-inoculated systems (A-3D-RBCs) under polypropylene microplastic (PP-MP) exposure. H-3D-RBCs showed superior resistance to PP-MP disturbance, with total nitrogen removal decreasing by only 14 %, compared with an approximately 60 % decline in A-3D-RBCs. Respiratory activity inhibition remained below 15 % in H-3D-RBCs but exceeded 90 % in A-3D-RBCs. 16S rRNA gene sequencing showed that PP-MP reduced species richness and diversity in A-3D-RBCs and was associated with a > 90 % loss of core denitrifying genera, including Corynebacterium and Pseudoxanthomonas, whereas H-3D-RBCs maintained community stability and enriched Pseudoxanthomonas to 13.8 %. Metagenomic analysis indicated that PP-MP impaired nitrification and denitrification potential in A-3D-RBCs, as reflected by decreased genes encoding AMO and HAO, a 51.78 % decrease in nosZ abundance, and enhanced dissimilatory nitrate reduction to ammonium (DNRA), which likely intensified competition with denitrification and promoted nitrogen conversion to ammonia. In contrast, H-3D-RBCs suppressed DNRA and maintained high nosZ abundance. Untargeted metabolomics further showed that PP-MP was associated with metabolic disorders in A-3D-RBCs, especially disruptions in alanine, aspartate, and glutamate metabolism and arginine biosynthesis, whereas H-3D-RBCs preserved these key nitrogen metabolic processes. Overall, this study identifies key vulnerabilities of nitrogen-removal biofilms under PP-MP disturbance and provides multi-omics evidence to support the development of microplastic-resistant biofilm wastewater treatment systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135159"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced lignin-to-lipid bioconversion via glucose-assisted co-metabolism in newly isolated bacteria.","authors":"Yongbing Li, Siyi Yang, Jiali Wang, Sihu Zhang, Canfang Tang, Wen Cao, Liejin Guo","doi":"10.1016/j.biortech.2026.135160","DOIUrl":"10.1016/j.biortech.2026.135160","url":null,"abstract":"<p><p>Lignin-to-lipid bioconversion is a promising approach for producing biodiesel precursors from renewable biomass, but is limited by low conversion efficiency and the scarcity of efficient microbial strains. Here, three newly isolated bacterial strains (L77, L78, and L90) were evaluated for lipid production from dealkaline lignin and lignin-derived aromatic compounds. All strains showed better growth and lipid accumulation on lignin-derived aromatics than on polymeric lignin. Glucose-assisted co-metabolism markedly improved lignin degradation and lipid biosynthesis. The maximum lipid titers reached 373.3±17.0 mg/L for L77, 527.0±11.8 mg/L for L78, and 223.3±5.4 mg/L for L90, while the highest lipid content was obtained with L78 (65.3±2.7%). Fatty acid profiling showed that C16:0 and C18:0 were the dominant components, accounting for approximately 70% of total fatty acids, indicating favorable properties for biodiesel production. These results expand the diversity of bacterial resources for lignin valorization and demonstrate the potential of newly isolated strains for sustainable microbial lipid production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135160"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ sludge reduction assisted by alternating electrode-based electrochemical treatment.","authors":"Xiaolei Zhang, Quanze Liu, Shaoping Luo, Yingxue Sun, Yong Li, Wenyi Dong, Patrick Drogui, Ji Li","doi":"10.1016/j.biortech.2026.135135","DOIUrl":"10.1016/j.biortech.2026.135135","url":null,"abstract":"<p><p>The massive production of sludge from municipal wastewater treatment plants poses a significant challenge to its disposal and management. This study proposes a sustainable in situ sludge reduction technology. A sequencing batch reactor (SBR) was used to investigate the process feasibility. The activated sludge from the SBR was first subjected to pH adjustment to dissociate the extracellular polymeric substances (EPS) of the microorganisms, which serve as a protective layer. The vulnerable microorganisms are then treated by electrochemical oxidation, which disrupts the cells and releases the organic matter. Equal volumes of sludge were treated at pH 2 and 12, respectively, and combined for 20 min electrochemical treatment at 15 V with a 2 cm electrode spacing, a 3 mL dosage of 1 M CaCl₂, and a stirring speed of 500 rpm. Around 22 % sludge disruption has been obtained. Alternating polarity has been shown to effectively prevent electrode passivation, achieving a 25 % improvement in sludge disruption. The treated sludge solution was then reintroduced into the SBR for metabolic assimilation. The results indicate that the SBR process can maintain a self-sustaining condition with up to 10 % sludge recirculation volume. The study provides new insights into in situ sludge reduction, with promise for widespread application and further optimization in future wastewater treatment systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135135"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing pyrite-based bioretention systems under complex stormwater conditions using a mixed carbon source strategy: Insights into complementary carbon release, microbial restructuring, and pyrite regeneration.","authors":"Hao Zheng, Haiyuan Ma, Zheng Kong, Wenlin Zhao, Xinyue Wang, Huan Xiao, Yuanxiang Mao, Shihu Hu, Hongxiang Chai","doi":"10.1016/j.biortech.2026.135181","DOIUrl":"10.1016/j.biortech.2026.135181","url":null,"abstract":"<p><p>Pyrite-based bioretention systems hold substantial promise for the simultaneous removal of nitrogen and phosphorus from stormwater runoff. However, their performance under high-frequency and high-intensity rainfall conditions is often limited by the low electron-supply capacity of pyrite. To address this limitation, a mixed carbon source strategy was developed to enhance nutrient removal under complex rainfall conditions. Four bioretention systems, including the mixed carbon source-pyrite system (WCP), woodchip-pyrite system, corncob-pyrite system, and pyrite-only system, were constructed and systematically evaluated under simulated rainfall events with varying intensities and frequencies. Among all systems, WCP consistently achieved the best performance, with removal efficiencies of 88.1 ± 1.3% for ammonium, 86.6 ± 1.9% for nitrate, 87.0 ± 2.0% for total dissolved nitrogen, and 83.3 ± 4.3% for total dissolved phosphorus. Mechanistic analyses revealed that readily biodegradable carbon released from corncob promoted rapid heterotrophic denitrification during rainfall events, whereas recalcitrant carbon released from woodchips sustained pyrite-driven autotrophic denitrification during drying periods. Their complementary carbon-release characteristics synergistically enhanced nitrogen and phosphorus removal. In addition, mixed carbon sources reshaped the microbial community, enhanced cooperation between autotrophic and heterotrophic functional groups, and improved nitrogen transformation and electron transfer efficiency. Media characterization further showed that mixed carbon sources promoted FeS/FeS₂ cycling, thereby facilitating pyrite regeneration and supporting long-term operational stability. Overall, this study elucidates a multi-dimensional enhancement mechanism driven by mixed carbon sources-complementary carbon release, microbial restructuring, and pyrite regeneration-and provides a robust and sustainable strategy for nutrient control in stormwater bioretention systems under complex rainfall conditions.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135181"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148256618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}