Bioresource Technology最新文献

Corrigendum to “Two-step biocatalytic conversion of post-consumer polyethylene terephthalate into value-added products facilitated by genetic and bioprocess engineering” [Bioresour. Technol. 417 (2025) 131837]

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-02 DOI: 10.1016/j.biortech.2025.132453

Gina Welsing , Birger Wolter , Greta E.K. Kleinert , Frederike Göttsch , Werner Besenmatter , Rui Xue , Alessandra Mauri , Dominik Steffens , Sebastian Köbbing , Weiliang Dong , Min Jiang , Uwe T. Bornscheuer , Ren Wei , Till Tiso , Lars M. Blank

引用次数: 0

Engineered stable partial nitrification/endogenous partial denitrification-anammox process for enhanced nitrogen removal from low carbon-to-nitrogen ratio wastewater

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-30 DOI: 10.1016/j.biortech.2025.132466

Mingchang Gao , Shaofang Sun , Changtao Shao , Qi Qiu , Congcong Kong , Liping Qiu

{"title":"Engineered stable partial nitrification/endogenous partial denitrification-anammox process for enhanced nitrogen removal from low carbon-to-nitrogen ratio wastewater","authors":"Mingchang Gao , Shaofang Sun , Changtao Shao , Qi Qiu , Congcong Kong , Liping Qiu","doi":"10.1016/j.biortech.2025.132466","DOIUrl":"10.1016/j.biortech.2025.132466","url":null,"abstract":"<div><div>Addressing the intractable challenges of nitrite instability and slow start-up in anammox for low carbon-to-nitrogen (C/N) ratio wastewater treatment, a one-stage partial nitrification/endogenous partial denitrification-anammox (PN/EPD-A) process in a sequencing batch biofilm reactor was proposed. By synergistically coupling PN and EPD, self-sustained nitrite supply for anammox was achieved. Concurrently, a layered biofilm structure, engineered through tailored aeration and carrier addition, facilitated the rapid enrichment of anammox bacteria. The results demonstrated exceptional performance, achieving a total nitrogen removal efficiency of 83.3 %, with anammox consistently contributing 75.8 % of the nitrogen removed. Microbial community analysis further indicated the stable coexistence of anammox bacteria, ammonia-oxidizing bacteria, and glycogen-accumulating organisms, with their relative abundance reaching 1.36 %, 2.19 % and 9.80 %, respectively. These findings unveiled a robust and efficient strategy to overcome the limitations of anammox technology in low C/N wastewater treatment, paving the way for its broader application in nitrogen removal.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132466"},"PeriodicalIF":9.7,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of aerobic denitrification system to long-term stress of sulfamethoxazole, erythromycin and ofloxacin in oligotrophic water

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-30 DOI: 10.1016/j.biortech.2025.132458

Yiling Di , Wanying Li , Rui Huo , Chenbin Wu , Yuting Zhao , Kun Shi , Shilei Zhou , Chun Liu

{"title":"Response of aerobic denitrification system to long-term stress of sulfamethoxazole, erythromycin and ofloxacin in oligotrophic water","authors":"Yiling Di , Wanying Li , Rui Huo , Chenbin Wu , Yuting Zhao , Kun Shi , Shilei Zhou , Chun Liu","doi":"10.1016/j.biortech.2025.132458","DOIUrl":"10.1016/j.biortech.2025.132458","url":null,"abstract":"<div><div>The responses of aerobic denitrification system to long-term stress of sulfamethoxazole (SMX), erythromycin (ERY) and ofloxacin (OFL) were investigated under low, middle, and high antibiotics stress. Findings showed that the aerobic denitrification performance was enhanced by antibiotics, and was higher than control system. Meanwhile, nitrate removal efficiency reached 43.01 ∼ 53.82 %, 29.48 ∼ 47.83 %, and 19.53 ∼ 34.08 % from low to high antibiotics stress in low carbon/nitrogen (C/N) ratio water. The <em>α</em>-diversity (ACE, Chao, and Shannon index) tended to increase for middle and high antibiotic stress. The PCoA and NMDS indicated that communities exhibited significantly difference (<em>P</em> < 0.001) under different antibiotics stress, which were agreement with the results of Adonis and ANOSIM. <em>Acidovorax</em>, <em>Zoogloea</em>, <em>Bdellovibrio</em>, <em>Paracoccus</em>, <em>Piscinibacter</em>, <em>Pseudomonas</em>, and <em>Rhizobacter</em> dominated the aerobic denitrification bacteria. Moreover, stochastic processes decreased gradually with increasing antibiotics. Furthermore, the results of network demonstrated that increase of antibiotics concentration could obviously reduce the microbial stability.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132458"},"PeriodicalIF":9.7,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorizing Hydrothermal liquefaction aqueous phase via nanofiltration: Enhancing biocrude production from algal biomass.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132456

Tiago Costa, Lance Schideman, Nandakishore Rajagopalan, Yuanhui Zhang

引用次数: 0

Simultaneous removal of nitrogen and phosphorus by aerobic denitrifying Paracoccus versutus JUST-3

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132457

Haoyun Wang , Yang Sun , Xingkun Zhou , Chengyuan Zhu , Xiujie Wang , Haq Nawaz Abbasi , Hongya Geng , Guangcan Zhu , Xingang Wang , Hongliang Dai

{"title":"Simultaneous removal of nitrogen and phosphorus by aerobic denitrifying Paracoccus versutus JUST-3","authors":"Haoyun Wang , Yang Sun , Xingkun Zhou , Chengyuan Zhu , Xiujie Wang , Haq Nawaz Abbasi , Hongya Geng , Guangcan Zhu , Xingang Wang , Hongliang Dai","doi":"10.1016/j.biortech.2025.132457","DOIUrl":"10.1016/j.biortech.2025.132457","url":null,"abstract":"<div><div>Strain JUST-3, exhibiting high-efficiency simultaneous nitrogen and phosphorus removal under aerobic conditions, was isolated and identified as <em>Paracoccus versutus</em> based on 16S rDNA gene sequencing and comprehensive physiological and biochemical analysis. The strain demonstrated optimal performance when cultured with sodium acetate as carbon source under the following conditions: C/N ratio of 10, P/N ratio of 0.2, 35 °C, and pH of 8.0. The variations in intermediate metabolites, the activity of functional enzymes, and the nitrogen/phosphorus balance experiments elucidated the pathways in nitrogen and phosphorus removal under aerobic conditions. Exogenous signal molecules (＜50 nmol/L) could promote growth, enhance aerobic denitrification, and improve simultaneous nitrogen and phosphorus performance. The identification of signaling molecules represents a significant breakthrough, revealing novel regulatory mechanisms in microbial quorum-sensing systems and enabling precise control of microbial community behaviors. This study expands the application of aerobic denitrification and phosphorus removal technology, laying the foundation for wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132457"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on short-chain fatty acids production from anaerobic fermentation of waste activated sludge pretreated by alkali-activated ammonium persulfate

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132461

Qiang Wang , Heng An , Tianqi Ruan , Xinyi Lu , Dongru Qiu , Zhenbin Wu , Qiaohong Zhou , Enrong Xiao

{"title":"Study on short-chain fatty acids production from anaerobic fermentation of waste activated sludge pretreated by alkali-activated ammonium persulfate","authors":"Qiang Wang , Heng An , Tianqi Ruan , Xinyi Lu , Dongru Qiu , Zhenbin Wu , Qiaohong Zhou , Enrong Xiao","doi":"10.1016/j.biortech.2025.132461","DOIUrl":"10.1016/j.biortech.2025.132461","url":null,"abstract":"<div><div>As a sustainable method for carbon recovery from waste activated sludge (WAS), anaerobic fermentation to produce short-chain fatty acids (SCFAs) is often limited by disintegration of WAS. A novel pretreatment method of alkaline-activated ammonium persulfate (AP/Alk), employing an initial pH of 10 and an ammonium persulfate dosage of 1 mM/g VSS (mmol per gram volatile suspended solids), was proposed in this study to enhance disintegration of WAS and yield of SCFAs. It was compared with one control and five pretreatment groups including alkali, persulfate, free ammonia, ammonium persulfate, alkali-activated sodium persulfate to elucidate the synergistic effects of free ammonia and radicals in WAS dissolution and acidogenesis within the AP/Alk system. The highest sludge disintegration degree with 30.3 % and maximum SCFAs production with 295.4 mg COD/g VSS were achieved by using the method. Comparative analysis showed that free ammonia primarily disrupted microbial cells to release intracellular organics, while radicals preferentially degraded tightly bound extracellular polymeric substances (TB-EPS) proteins. The synergistic effects of free ammonia and radicals accelerated accumulation of soluble proteins and polysaccharides, improved selectively enrichment of hydrolytic-acidogenic genera (e.g., <em>Macellibacteroides</em>, <em>Proteiniclasticum</em>, <em>Desulfobulbus</em>), and upregulated antioxidant genes to alleviate oxidative stress, but suppressed SCFAs consumers (e.g., <em>unclassified_f__Comamonadaceae</em>) including methanogens (e.g., <em>Methanosaeta</em>), thereby promoting the accumulation of SCFAs and acetic acid proportion. AP/Alk offers a sustainable strategy for WAS utilization and energy recovery.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132461"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132460

Chaowei Zhou , Yingying Zhu , Peifang Ren , Jing Leng , Xuan Xia , Tianpeng Chen , Wenjun Sun , Pengpeng Yang , Huanqing Niu , Yong Chen , Hanjie Ying

{"title":"Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis","authors":"Chaowei Zhou , Yingying Zhu , Peifang Ren , Jing Leng , Xuan Xia , Tianpeng Chen , Wenjun Sun , Pengpeng Yang , Huanqing Niu , Yong Chen , Hanjie Ying","doi":"10.1016/j.biortech.2025.132460","DOIUrl":"10.1016/j.biortech.2025.132460","url":null,"abstract":"<div><div>The ever-growing demand for cost-effective and green biocatalytic transformations has prompted the rational design and development of robust biocatalytic tools. However, transformations are hindered by limited continuous process and enzymatic instability. Here, 10 Flo family related genes in <em>Komagataella phaffii</em> were systematically evaluated to assess their adhesive properties. For the first time, we identified the KpFlo11C domain of BSC1p as facilitating cell aggregation on carriers, thereby enhancing the biofilm immobilization process. Furthermore, an engineered <em>K. phaffii</em> strain, fixing β-galactosidase on the cell surface, was constructed by optimizing the signal peptide and gene dosage, for enhancing the efficiency of enzyme targeting and anchoring, as well as the proportion of cells displaying the enzyme. Finally, the KpFlo11C domain was overexpressed in this <em>K. phaffii</em> cell display system to construct the enzyme-cell@material biocatalyst, which exhibited robust continuous production of galacto-oligosaccharides (GOS) at a rate of 8.16 g/L/h in a 6-L fermenter. The development of this enzyme-cell@material biocatalyst, which offers a highly efficient, stable, low-cost, and simplified biocatalytic process, provides a direction for the application of other yeasts in large-scale industrial continuous production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132460"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of inorganic carbon on purple phototrophic bacteria polyhydroxyalkanoates production under high reductive stress environment.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132462

Juliana Roda Almeida, Maria Ascensão Miranda Reis, Joana Costa Fradinho

{"title":"Influence of inorganic carbon on purple phototrophic bacteria polyhydroxyalkanoates production under high reductive stress environment.","authors":"Juliana Roda Almeida, Maria Ascensão Miranda Reis, Joana Costa Fradinho","doi":"10.1016/j.biortech.2025.132462","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132462","url":null,"abstract":"<p><p>Fermented wastes rich in reduced compounds challenge anaerobic purple phototrophic bacteria (PPB) systems by causing redox imbalances. This study evaluated polyhydroxyalkanoates (PHA) production and CO<sub>2</sub> fixation by PPB mixed cultures as means to balance internal redox, under four inorganic carbon (IC) availability conditions in a sequencing batch reactor. Culture selection under permanent IC presence promoted higher microbial diversity, but, strongly dependent on IC to balance internal redox, even when more oxidized substrates were supplemented. Increasing IC limitation favoured Rhodopseudomonas palustris, revealing its redox balancing capability independently of IC fixation. PHA contents of 20-29% gPHA/gVSS were achieved across all IC availabilities, however, when IC was present growth was promoted over PHA production, indicating a preferable electron balance through CO<sub>2</sub> fixation. Nevertheless, cultures selected under feast-famine IC exhibited a preference for PHA accumulation under IC limitation, suggesting IC tuning as a potential growth-controlling strategy to boost phototrophic PHA production using reduced feedstocks.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132462"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Neurospora crassa for the production of xylitol and ethylene glycol from xylose

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132459

Caihong Ma , Jiacheng Liang , Hao Fang , Zi Wei Luo , Shaolin Chen , Chen Zhao

{"title":"Metabolic engineering of Neurospora crassa for the production of xylitol and ethylene glycol from xylose","authors":"Caihong Ma , Jiacheng Liang , Hao Fang , Zi Wei Luo , Shaolin Chen , Chen Zhao","doi":"10.1016/j.biortech.2025.132459","DOIUrl":"10.1016/j.biortech.2025.132459","url":null,"abstract":"<div><div>The use of pentose from lignocellulose is critical for economic production of biofuels and chemicals. In this study, a filamentous fungus, <em>Neurospora crassa</em>, was used to metabolize xylose and synthesize xylitol and ethylene glycol (EG). Firstly, xylulose kinase gene was knocked out to prevent excessive xylose usage for strain growth, resulting in 5.8 ± 0.3 g/L xylitol and 58.3 ± 2.6 mg/L EG. Through optimization, the xylitol accumulation in the hydrolysate containing xylose reached 62.0 ± 0.6 g/L. The expression of 6-phosphofructokinase increased EG production with a maximum titer of 102.1 ± 1.8 mg/L, proving that the strain synthesized EG through xylulose-1-phosphate pathway. Addition of furfural or hydrolysate, and oxygen-limited environment were conducive to the EG accumulation. Further optimized strain accumulated EG up to 2586.4 ± 198.9 mg/L. This study provided the evidence of an all-natural metabolic pathway for EG synthesis in <em>N. crassa</em> and also demonstrated a new chassis cell for synthesis of xylitol and EG from xylose-rich hydrolysate.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132459"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient nepetalactone production in Saccharomyces cerevisiae via metabolic engineering and bioprocess optimization.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-28 DOI: 10.1016/j.biortech.2025.132440

Herong Wang, Mengying Shan, Qi Gao, Jia Wang, Ruixuan Zhang, Ying Wang, Mingdong Yao, Wenhai Xiao

{"title":"Efficient nepetalactone production in Saccharomyces cerevisiae via metabolic engineering and bioprocess optimization.","authors":"Herong Wang, Mengying Shan, Qi Gao, Jia Wang, Ruixuan Zhang, Ying Wang, Mingdong Yao, Wenhai Xiao","doi":"10.1016/j.biortech.2025.132440","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132440","url":null,"abstract":"<p><p>Nepetalactone, a natural insect repellent comparable to N,N-diethyl-meta-toluamide (DEET), is challenging to produce through plant extraction or chemical synthesis. This study achieved the de novo synthesis of nepetalactone in Saccharomyces cerevisiae without expensive precursors or inducers. Initially, the metabolic pathway for nepetalactone synthesis was successfully established in Saccharomyces cerevisiae. A metabolic pathway was established using strategies such as iridoid synthase (ISY) source screening, enzyme fusion, and cofactor regeneration to optimize nepetalactone production. Bioprocess optimization through chromosomal integration and two-phase fermentation prevented its conversion to dihydronepetalactone, resulting in a high-yield strain, NTE21, with a titer of 2.5 g/L. A record titer of 4.5 g/L was achieved in 5.0 L fed-batch fermentation via continuous batch feeding. This study documents the potential of microbial platforms for the sustainable, cost-effective, and scalable production of nepetalactone, paving the way for its commercial application as a natural insect repellent.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132440"},"PeriodicalIF":9.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0