Bioresource Technology最新文献

{"title":"Performance and underlying mechanisms of zero-valent iron and percarbonate co-regulation for improved volatile fatty acids production from food waste anaerobic fermentation","authors":"Qin Zhang ,&nbsp;Yuke Xu ,&nbsp;Han Yang ,&nbsp;Minjun Zhao ,&nbsp;Wenzhuo Li","doi":"10.1016/j.biortech.2025.132645","DOIUrl":"10.1016/j.biortech.2025.132645","url":null,"abstract":"<div><div>Anaerobic biorefining of food waste (FW) into volatile fatty acids (VFAs) is typically limited by substrate recalcitrance and acid-induced stress. In this study, co-regulation with percarbonate (SPC) and zero-valent iron (ZVI) resulted in a maximum VFAs concentration of 28,317.9 mg COD/L, compared to only 3,986.4 mg COD/L in the control. SPC/ZVI treatment facilitated FW solubilization, enhanced substrate biodegradability, and alleviated acid inhibition. These changes promoted the enrichment of functional bacteria (<em>e.g., Megasphaera</em> and <em>Clostridium</em>) and stimulated key metabolic pathways and gene expression (<em>e.g., fabG</em> and <em>por</em>) involved in VFAs biosynthesis. Together with the provision of bioavailable organics and improved fermentation conditions, activation of stress defense systems in functional bacteria (<em>e.g., katG</em> and <em>kdpA</em>) counteracted the acid and oxidative stress in the SPC/ZVI system, thereby preserving metabolic activity for VFAs production. This study presents a dual modulation strategy to enhance FW fermentation, offering valuable insights for efficient resource recovery from FW.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132645"},"PeriodicalIF":9.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH adjustment increases biofuel production from inhibitory switchgrass hydrolysates","authors":"Lillian M. Barten ,&nbsp;Johnathan G. Crandall ,&nbsp;Dan Xie ,&nbsp;Jose Serate ,&nbsp;Evan Handowski ,&nbsp;Annie Jen ,&nbsp;Katherine A. Overmyer ,&nbsp;Joshua J. Coon ,&nbsp;Chris Todd Hittinger ,&nbsp;Robert Landick ,&nbsp;Yaoping Zhang ,&nbsp;Trey K. Sato","doi":"10.1016/j.biortech.2025.132651","DOIUrl":"10.1016/j.biortech.2025.132651","url":null,"abstract":"<div><div>Biofuels derived from renewable and sustainable lignocellulosic biomass, such as switchgrass, offer a promising means to limit greenhouse gas emissions. However, switchgrass grown under drought conditions contains high levels of chemical compounds that inhibit microbial conversion to biofuels. Fermentation of drought switchgrass hydrolysates by engineered <em>Saccharomyces cerevisiae</em> and <em>Zymomonas mobilis</em> results in lower ethanol production than does fermentation of hydrolyzed switchgrass from a typical rainfall year<em>.</em> Here, it is demonstrated that this inhibitory effect can be alleviated by altering the pH of drought switchgrass hydrolysates produced by two different pretreatment methods: Ammonia Fiber Expansion (AFEX) and Soaking in Aqueous Ammonia (SAA). Fermentation rates and biofuel production by <em>Saccharomyces cerevisiae</em> and <em>Zymomonas mobilis</em> were higher at pH 5.8 than at pH 5.0 from all feedstock years and following both pretreatment methods. SAA pretreatment of drought switchgrass furthermore enabled increased fermentation rates and biofuel titers compared to AFEX pretreatment. A synthetic mimic of switchgrass hydrolysate was developed and identified relief from pH-dependent inhibition by lignocellulose-derived inhibitors as the cause of increased biofuel production above a pH of 5.0. These results demonstrate that SAA pretreatment and pH adjustment can significantly improve fermentation and biofuel production from inhibitory feedstocks by industrial microorganisms.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"432 ","pages":"Article 132651"},"PeriodicalIF":9.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving ergothioneine content in Pleurotus citrinopileatus through two-stage oxidative stimulus strategy","authors":"Weiyi Li ,&nbsp;Yuwan Weng ,&nbsp;Huiguo Ma ,&nbsp;Xuechao Hu ,&nbsp;Lujing Ren","doi":"10.1016/j.biortech.2025.132630","DOIUrl":"10.1016/j.biortech.2025.132630","url":null,"abstract":"<div><div>Mushroom is an important agricultural crop, rich in various nutrients and bioactive compounds. Ergothioneine (EGT), a naturally occurring antioxidant, is found in mushrooms that offers significant health benefits. This study developed a two-stage oxidative stimulus strategy to enhance the EGT content. After screening 11 species of macrofungi, <em>P. citrinopileatus</em> 303 was selected. With the cultivation conditions optimized, EGT titer reached 533.93 mg/L. To further boost EGT synthesis, oxidative stress was introduced by hydrogen peroxide (H₂O₂). An optimized strategy combining oxidative stress with clearance was then implemented. Finally, a two-stage oxidative stimulus approach was employed, where the cells were first exposed to H₂O₂, followed by oxidative clearance using vitamin C in the second stage. This strategy increased the EGT titer to 641.76 mg/L, 5.83 times that of the initial group, with the fermentation time reduced to 5 days. It offered valuable insights for the industrial-scale biosynthesis of this potent antioxidant.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132630"},"PeriodicalIF":9.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mask-assisted jet biomachining based on Acidithiobacillus ferrooxidans and its application in functional friction surfaces","authors":"Jingyu Song ,&nbsp;Hui Huang ,&nbsp;Weibin Shi ,&nbsp;Xigui Wang ,&nbsp;Wenbin Zhong ,&nbsp;Wenhan Zeng ,&nbsp;Weimin Lin","doi":"10.1016/j.biortech.2025.132642","DOIUrl":"10.1016/j.biortech.2025.132642","url":null,"abstract":"<div><div>A novel mask-assisted jet biomachining (MJBM) method was developed to fabricate functional microstructures on a phosphor-tin alloy (ZCuSn10Pb1), significantly enhancing the surface tribological performance. MJBM integrates the metabolic activity of Acidithiobacillus ferrooxidans with directional jetting of the culture supernatant, achieving a material removal rate 58.94 times higher than immersion and 4.97 times higher than shaking methods, respectively, while maintaining notably lower dimensional deviations in the fabricated micro-dimples. Compared to smooth surfaces, the textured surfaces exhibited up to a 56.4 % reduction in the friction coefficient. These findings, in conjunction with current literature, indicate that MJBM can be a more is an environmentally friendly and sustainable approach for fabricating functional microstructures. Additionally, elucidating the material removal mechanisms underlying MJBM significantly broadens the application potential of bioprocesses. The innovative integration of MJBM with surface texturing and micro-component fabrication provides valuable insights for advancing cleaner production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132642"},"PeriodicalIF":9.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biosynthesis of 6-aminocaproic acid in engineered Escherichia coli with artificial protein cage-organized enzymatic cascades","authors":"Ruoshi Luo ,&nbsp;Lin Hu ,&nbsp;Dan Wang ,&nbsp;Kaixing Xiao ,&nbsp;Xuemei Liu ,&nbsp;Yaqi Kang ,&nbsp;Qinhong Wang","doi":"10.1016/j.biortech.2025.132641","DOIUrl":"10.1016/j.biortech.2025.132641","url":null,"abstract":"<div><div>Microbial synthesis of 6-aminocaproic acid (6-ACA), a key nylon-6 monomer, was the focus of this study. Our previous work on 6-ACA biosynthesis using an artificial iterative carbon-chain-extension cycle showed potential, but the impact of intermediates on metabolism remained unresolved. To address this, a bacterial microcompartment (BMC) was engineered in <em>Escherichia coli</em> to encapsulate 6-ACA synthesis enzymes, effectively controlling the release of intermediate products. This intervention led to a 90.85 % increase in cell growth and a final 6-ACA yield increase from 46.76 mg/L to 1.12 g/L in a 1 L fermentor. The redesigned BMC demonstrated potential in regulating cascade enzymatic catalysis, particularly in managing intermediates that could impact enzyme proteins, cause cytotoxicity, or DNA damage in cells. This work highlights the potential of the redesigned BMC in enhancing production by controlling the effects of intermediates on cellular processes.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132641"},"PeriodicalIF":9.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-sourcing from byproducts: A comprehensive review of bioactive molecules in Agri-Food Waste (AFW) streams for valorization and sustainable applications","authors":"Eman Shawky ,&nbsp;Simon Gibbons ,&nbsp;Dina A. Selim","doi":"10.1016/j.biortech.2025.132640","DOIUrl":"10.1016/j.biortech.2025.132640","url":null,"abstract":"<div><div>Agri-Food Waste (AFW) valorization represents a transformative approach to addressing global sustainability challenges by converting underutilized biomass into high-value bioactive compounds. This review offers a comprehensive examination of AFW streams—ranging from agricultural residues and post-harvest losses to food processing by-products—highlighting their composition, bioactive molecule content, and their untapped potential in various industrial applications. Special attention is given to the classification and health-promoting properties of key bioactives including polyphenols, carotenoids, dietary fibers, bioactive peptides, and lipids, emphasizing their roles in human nutrition, disease prevention, and functional product development. A critical evaluation of state-of-the-art extraction and recovery technologies is presented, covering solvent extraction, microwave-assisted extraction, enzyme-assisted extraction, solid-state fermentation, pressurized liquid extraction, supercritical fluid extraction, and the use of green solvents such as deep eutectic solvents (DES). The review further discusses the integration of these technologies into scalable, sustainable valorization pathways. Applications of waste-derived bioactives in the development of functional foods, beverages, dietary supplements, cosmetics, and pharmaceuticals are explored, along with the associated challenges including technical constraints, regulatory hurdles, and bioavailability issues. Emerging frameworks such as microalgae-based biorefineries are discussed for their potential in closed-loop circular economies. The manuscript also analyzes the environmental, economic, and societal implications of AFW valorization, offering insights into policy frameworks, life cycle assessments, market opportunities, and the role of innovation and cross-sector collaboration in promoting circular bioeconomy models. Ultimately, this review underscores the importance of AFW valorization as a pivotal strategy for sustainable development, resource efficiency, and the reduction of ecological footprints in the agri-food sector.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132640"},"PeriodicalIF":9.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications, life cycle assessment, and circular economy of bamboo torrefaction for sustainability: A state-of-the-art review","authors":"Wei-Hsin Chen ,&nbsp;Pin-Chun Liang ,&nbsp;Wei-Yi Wang ,&nbsp;Pen-Chi Chiang ,&nbsp;Jiří Ryšavý ,&nbsp;Jakub Čespiva ,&nbsp;Jo-Shu Chang","doi":"10.1016/j.biortech.2025.132629","DOIUrl":"10.1016/j.biortech.2025.132629","url":null,"abstract":"<div><div>This review comprehensively explores the characteristics and applications of torrefied bamboo. Bamboo has a high volatile matter (VM) content (73.9–93.0 %), which results in substantial liquid byproducts during torrefaction. The higher heating value (HHV) of biochar produced from wet torrefaction (WT) is greater than that made from dry torrefaction (DT). When the torrefaction severity factor is 8.7, the bamboo hydrochar’s HHV from WT can achieve 29.3 MJ⋅kg⁻¹, whereas bamboo biochar from DT only have 23.3 MJ⋅kg⁻¹. Bamboo vinegar and tar, byproducts from bamboo torrefaction, are effective biopesticides and have diverse applications, including polyurethane coatings and insecticides. Life cycle assessments reveal that bamboo-based building materials can reduce carbon footprints by 46.2 % to 87.6 % compared to traditional construction materials. Furthermore, bamboo materials are highly beneficial for the circular economy and environmental sustainability. In summary, bamboo biochar’s applications are extensive, and its derived products are commercially competitive and environmentally friendly.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132629"},"PeriodicalIF":9.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome catalog and dynamics of the Chinese liquor fermentation process","authors":"Xue Zhu ,&nbsp;Liangqiang Chen ,&nbsp;Pengshuo Yang ,&nbsp;Sha Luo ,&nbsp;Mengjing Teng ,&nbsp;Wenjie Zhu ,&nbsp;Yuxue Li ,&nbsp;Dan Zhao ,&nbsp;Nan Wang ,&nbsp;Xiaoke Chen ,&nbsp;Mingyue Cheng ,&nbsp;Huabin Tu ,&nbsp;Weihua Huang ,&nbsp;Fan Yang ,&nbsp;Li Wang ,&nbsp;Xin Liu ,&nbsp;Kang Ning","doi":"10.1016/j.biortech.2025.132620","DOIUrl":"10.1016/j.biortech.2025.132620","url":null,"abstract":"<div><div>Fermented food remains poorly understood, largely due to the lack of knowledge about microbes in food fermentation. Here, this study constructed Moutai Fermented Grain Catalog (MTFGC), a representative liquor fermented by one of the most complex fermentations. MTFGC comprised 8,379,551 non-redundant genes and 5,159 metagenome-assembled genomes, with 20% species and 20% genes being novel. Additionally, 25,625 biosynthetic gene clusters (BGCs) and 28 BGC-enriched species were identified. Moreover, the microbial community assembly was deterministic, with significant species and gene changes in early fermentation stages, while stabilizing in later stages. Further BGC-knockout experiments verified <em>Bacillus licheniformis</em>, a BGC-enriched species, employed its BGCs for synthesizing the aroma-related lipopeptide lichenysin. This study has established the largest genetic resource for fermented food, uncovering its uniqueness and high metabolic potential. These findings facilitate the transition potential from traditional fermentation to precision-driven synthetic biology in food systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132620"},"PeriodicalIF":9.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption and nitrogen removal of NH4+-N based on Mn (II)/α-MnO2 cycle in bio-electrochemical system","authors":"Zhanping Cao,&nbsp;Li Yan,&nbsp;Xinyue Duan,&nbsp;Zhengran Li,&nbsp;Xingyue Wang","doi":"10.1016/j.biortech.2025.132628","DOIUrl":"10.1016/j.biortech.2025.132628","url":null,"abstract":"<div><div>This paper developed a single-chamber α-MnO₂-coupled microbial electrolysis cell (α-MnO₂-MEC) system to enhance the oxidation denitrification rate of ammonia nitrogen (NH₄⁺-N) in order to overcome the electrode repulsion problem between NH₄⁺ and the anode. The α-MnO₂ material with an equilibrium adsorption capacity of 10.6 mg·g⁻¹ for NH₄⁺-N was developed. The removal rate of total nitrogen in the α-MnO₂-MEC reactor is 95.8 %, and NH₄⁺ oxidation efficiency is 100 % in 20 h, which is 78.7 % and 47.8 % higher than in the α-MnO₂ reactor and the MEC reactor, respectively. The Mn(II)/α-MnO₂ cycle was realized in α-MnO₂-MEC reactor, avoiding the loss of the Mn(II). The 16S rRNA gene sequencing identified key microbial genera involved in the ammonia removal are <em>Candidatus_Brocadia</em>, <em>SC-I-84</em>, and <em>Thauera</em>. This study demonstrates that combining α-MnO₂ with bioelectrochemistry provides a novel strategy for ammonia nitrogen wastewater treatment, offering a new insight for optimizing electrochemical-microbial coupled nitrogen removal.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132628"},"PeriodicalIF":9.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Corynebacterium glutamicum for efficient l-homoserine production","authors":"Guihong Zhao ,&nbsp;Yaqun Tang ,&nbsp;Zihan Li ,&nbsp;Geer Liu ,&nbsp;Dezhi Zhang ,&nbsp;Xiaoqing Hu ,&nbsp;Xiaoyuan Wang","doi":"10.1016/j.biortech.2025.132617","DOIUrl":"10.1016/j.biortech.2025.132617","url":null,"abstract":"<div><div><span>l</span>-homoserine is an important precursor in synthesizing the essential amino acids derived from <span>l</span>-aspartate and other valuable bio-based products, and is widely used in cosmetics, food, and pharmaceutical industries. However, the yield of <span>l</span>-homoserine in <em>Corynebacterium glutamicum</em> is limited. In this study, we successfully engineered a <em>C</em>. <em>glutamicum</em> to efficiently produce <span>l</span>-homoserine. First, a <span>l</span>-threonine-producing strain TWZ023 was optimized by knocking out <em>thrB</em>, resulting in the strain HWZ006 which could produce 20.8 g/L <span>l</span>-homoserine with a yield of 0.231 g/g glucose. Then, the 240th Arg residue of homoserine kinase (HK) in TWZ023 was identified as an important site for <span>l</span>-homoserine binding. Subsequently, the optimal mutant strain R240I was screened through site-directed saturation mutagenesis of HK in TWZ023, it could produce 26.8 g/L <span>l</span>-homoserine with a yield of 0.298 g/g glucose. The HK-homoserine binding mechanism was analyzed by using molecular docking, further providing some potential mutation sites. The final strain R240I/pXTuf-<em>Cgl2344</em> was obtained by optimizing the efflux of <span>l</span>-homoserine, it could produce 29.9 g/L <span>l</span>-homoserine with a yield of 0.332 g/g glucose in shake flasks. In a 15 L fermenter, the final strain produced 78.3 g/L with a yield of 0.28 g/g glucose. These metabolic engineering strategies used in this study have fundamentally enhanced the ability of <em>C</em>. <em>glutamicum</em> to produce <span>l</span>-homoserine.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132617"},"PeriodicalIF":9.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}