Bioresource Technology最新文献

{"title":"Efficient bioconversion of corn stover hydrolysates into phenazine-1-carboxylic acid by engineered Pseudomonas chlororaphis for sustainable biopesticide production","authors":"Sheng-Jie Yue ,&nbsp;Yu-Xuan Li ,&nbsp;Kai-Quan Liu ,&nbsp;Sheng-Xiao Zhang ,&nbsp;Peng Huang ,&nbsp;Wei Wang ,&nbsp;Xue-Hong Zhang ,&nbsp;Xiang-Rui Hao ,&nbsp;Lian Jiang ,&nbsp;Hong-Bo Hu","doi":"10.1016/j.biortech.2025.133371","DOIUrl":"10.1016/j.biortech.2025.133371","url":null,"abstract":"<div><div>The conversion of lignocellulose into value-added chemicals provides a promising solution for replacing petrochemical resources, reducing environmental impact, and promoting the transition to green production. The hydrolysis products of lignocellulosic biomass are abundant in glucose, xylose, and arabinose, and the efficient conversion of these sugars through various biotechnological processes is crucial for maximizing the economic and environmental benefits of lignocellulosic biomass as a renewable resource. In this work, we engineered a strain of <em>Pseudomonas chlororaphis</em> that efficiently produces phenazine-1-carboxylic acid (PCA), a biopesticide registered in China as ’Shenqinmycin’, from corn stover hydrolysates. The genome-scale metabolic model of <em>P. putida</em> (iJN1462) was adapted for <em>P. chlororaphis</em> GP72 by incorporating two additional pathways. Using this adapted model, we engineered the strain <em>P. chloriraphis</em> GP72, which completely consumed all the sugars in corn stover hydrolysate and converted them into PCA, with a yield of 384.2 mg/L. This is the first report to display the full utilization of sugars in corn stover hydrolysate for PCA production. This work demonstrates the potential of <em>P. chlororaphis</em> in utilizing corn stover hydrolysate for PCA biosynthesis and provides valuable references for the biosynthesis of other value-added chemicals in lignocellulosic biomass using <em>Pseudomonas</em>.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133371"},"PeriodicalIF":9.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-aeration strategy for improved biogas yield and in-situ hydrogen sulfide mitigation during high-solids co-digestion of poultry litter","authors":"Bhavya Karumanchi, Sameena Begum, Gangagni Rao Anupoju, Biplob Kumar Pramanik","doi":"10.1016/j.biortech.2025.133399","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133399","url":null,"abstract":"High-solids anaerobic digestion sustainably converts organic waste into methane-rich biogas and nutrient-rich biomanure, offering dual benefits. This study assessed poultry litter (PL) digestion at 10 %, 30 %, and 60 % total solids (TS) under three setups: (i) PL alone, (ii) co-digestion with food waste hydrolysate (PL + FH) to improve carbon/nitrogen (C/N) ratio, and (iii) in situ desulfurization (St-PL) using sulfur-oxidizing bacteria (SOB) with micro-aeration to reduce H<ce:inf loc=\"post\">2</ce:inf>S. The highest biogas yields were obtained at 30 % TS: 12.4 L (PL + FH), 9.9 L (PL), and 8.9 L (St-PL). Methane content was highest in St-PL (68 %) with H<ce:inf loc=\"post\">2</ce:inf>S removal of 80–92 %. However, slight sulfate accumulation was detected in the digestate, attributed to the metabolic activity of sulfur oxidizing bacteria (SOB). Microbial analysis revealed the dominance of <ce:italic>Firmicutes</ce:italic>, <ce:italic>Bacteroidetes, Methanosaeta, and Methanobacterium</ce:italic>. This study demonstrates a scalable strategy in which micro-aeration combined with SOB enhanced methane yield and biogas quality while effectively mitigating H2S during high-solids poultry litter digestion.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"4 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobubble-enabled foam fractionation to remove freshwater microalgae and microcystin","authors":"Yihan Zhang ,&nbsp;Lili Li ,&nbsp;Shan Xue ,&nbsp;Tsung-Ta David Hsu ,&nbsp;Anne C. Hurley ,&nbsp;Meiyin Wu ,&nbsp;Xuezhi Zhang ,&nbsp;Wen Zhang","doi":"10.1016/j.biortech.2025.133351","DOIUrl":"10.1016/j.biortech.2025.133351","url":null,"abstract":"<div><div>Climate change and water pollution intensify algal blooms in natural lakes and reservoirs, leading to significant water quality challenges. Effectively removing excessive microalgae and algal toxins or micropollutants is essential for improving water quality, preserving aesthetic value, and protecting human health. This study evaluated the use of nanobubble-enabled foam fractionation for the removal of <em>Microcystis aeruginosa</em> and three microcystin congeners (MC-LR, MC-RR, and MC-YR). Foam was generated using air nanobubbles combined with surfactants—cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and chitosan—to enhance foamability and stability. Among the tested surfactants, CTAB exhibited the highest foamability, yielding improved removal performance. When CTAB concentration increased from 40 to 80 mg·L⁻¹, algal removal efficiency slightly improved from 60.0 ± 5.2 % to 69.0 ± 2.6 %, while the concentration factor declined from 4.45 to 3.37 due to enhanced frothing. Increasing salinity from 10 mM to 100 mM significantly reduced the concentration factor from 3.9 ± 0.1 to 0.9 ± 0.5, indicating that elevated ionic strength impairs foam-based separation of algal biomass. Removal of microcystins was influenced by both the presence of microalgae and surfactant conditions. MC-RR showed higher removal in the absence of microalgae (43.2 ± 1.5 % to 56.8 ± 2.0 %) compared to that (27.0 ± 5.6 % to 28.3 ± 3.3 %) when algae was present, suggesting competition during foam separation. Among the three congeners, the most hydrophobic MC-LR exhibited the highest foam affinity, with a removal efficiency of 77.4 ± 9.2 %. Test results from real lake water suggest that the complex water matrixes (e.g., salinity and dissolved organic matters) slightly affected foaming ability and reduced removal rates of target algal pollutants, especially at lower surfactant doses. These findings provide new insights into integrating nanobubble-enhanced foam fractionation into water treatment systems for mitigating harmful algal blooms (HABs) and microcystins.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133351"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming elemental sulfur bioavailability limitations with sponge iron coupling for enhanced nitrate removal: Novel perspective on electron transfer and iron-nitrogen-sulfur metabolism.","authors":"Haohao Miao, Wei Zeng, Mengjia Zhan, Xiaojing Hao, Ruikang Wang, Yongzhen Peng","doi":"10.1016/j.biortech.2025.133390","DOIUrl":"10.1016/j.biortech.2025.133390","url":null,"abstract":"<p><p>This study developed a novel sponge iron (sFe⁰) and elemental sulfur (S⁰) coupled autotrophic denitrification biofilter (S⁰-sFe⁰AD). At the ratio Fe⁰ to S⁰ of 0.5 and a hydraulic retention time of 1.5 h, the system achieved high nitrate and phosphate removal rates of 969.7 mgN·L^-1·d^-1 and 56.1 mgP·L^-1·d^-1, with efficiencies over 98.5 %. Activated biochemical sulfidogenic pathways enabled in situ regeneration of highly bioavailable FeS. This process not only expanded electron donor pool but also reduced sulfate production by facilitating multi-pathway denitrification. Moreover, FeS-mediated direct extracellular electron transfer promoted iron-sulfur redox cycling. Metagenomic analysis further revealed enhanced iron and energy metabolism within the coupled system. The enriched iron-sulfur redox bacteria (Thiobacillus, Desulfurivibrio and Geothrix) and genes (narB, mtrC, sox, fccAB and sir) facilitated the establishment of a self-sustaining iron-sulfur cycle, thereby extending system longevity. This study provides novel insights for developing efficient iron-sulfur coupled autotrophic denitrification technology for sustainable wastewater treatment.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133390"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel combined Enzymatic-Thermal hydrolysis process for mass reduction and resource recovery from waste activated sludge.","authors":"Lin Li, Heyuan Lv, Lequn Qu, Xueqing Shi","doi":"10.1016/j.biortech.2025.133387","DOIUrl":"10.1016/j.biortech.2025.133387","url":null,"abstract":"<p><p>As the generation of waste activated sludge (WAS) increases and the problem of resource scarcity worsens, the demand for sustainable sludge disposal and resource recovery technologies is growing rapidly. In this study, a novel combined enzymatic-thermal hydrolysis process was assessed for enhancing mass reduction and resource recovery from WAS. Heating temperature, as the key parameter was optimized. With combined enzymatic-thermal hydrolysis, a maximum SCOD concentration of 48,619 mg/L was achieved in combined hydrolysis liquid (CHL) under an optimum temperature of 165 ℃ (CHL₁₆₅). The concentration of PS and PN in CHL₁₆₅ were 4.4 % and 11.1 % higher than that in thermal hydrolysis liquid (THL) at 165 ℃ (THL₁₆₅). Meanwhile, the contents of heavy metals (Hg, As, Cd, and Cr) in CHL were all below 0.5 mg/L, indicating that the application posed an extremely low risk to the ecological environment and human health. Comparing with raw WAS, the mass reduction rate of up to 28.3 % was achieved. Moreover, the utilization of CHL₁₆₅ as a carbon source to facilitate nitrate nitrogen (NO₃^--N) removal in wastewater treatment resulted in the efficiency reaching 94.0 % of that achieved with commercial sodium acetate. Accordingly, the CHL₁₆₅ played a prominent role as a carbon source with slow-release effect for denitrification in reducing the cost of NO₃^--N removal. The above research will provide a new direction for the advanced resource utilization of WAS.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133387"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-stage hollow-fiber membrane bioreactor for wastewater treatment and resource recovery through hydrogen-driven mixotrophic nitrogen assimilation.","authors":"Manoj Kumar, Chiara Belloni, Stefano Papirio, Francesco Pirozzi, Giovanni Esposito, Silvio Matassa","doi":"10.1016/j.biortech.2025.133398","DOIUrl":"10.1016/j.biortech.2025.133398","url":null,"abstract":"<p><p>The growing need to recover nutrients from wastewater pushes towards the development of new biological processes enabling the removal and upcycling of nitrogen (N) through microbial assimilation. In this study, a one-stage H₂-fed aerobic hollow-fiber membrane bioreactor, seeded with a mixed culture of hydrogen-oxidizing bacteria (HOB), was developed to treat a synthetic urban wastewater below discharge limits in terms of chemical oxygen demand (COD) and ammonium nitrogen (N-NH₄⁺). The removal and recovery of the latter as microbial protein (MP) through mixotrophic N-assimilation, together with the avoidance of greenhouse gas emissions (CO₂, N₂O) during wastewater treatment, were the main objectives of this study. The performance of the H₂-driven mixotrophic N-assimilation process was evaluated in continuous mode by studying the influence of the hydraulic retention time (HRT), COD_H2:COD_acetate and H₂:O₂ ratios in the mixotrophic system. Under mixotrophic conditions, higher HRT and COD_H2:COD_acetate ratios ensured stable reactor performance with 484.4 mg VSS∙L^-1 biomass, 90 % nitrogen assimilation and up to 64.7 % protein content, whereas low HRT yielded a higher biomass concentration (604.9 mg VSS∙L^-1) but unstable performance. Conversely, under heterotrophic conditions, both nitrogen assimilation (40 %) and protein content (40 %) were significantly lower, while the residual nitrate concentration (16.1 mg∙L^-1 of N-NO₃^-) prevented compliance with total nitrogen discharge limits (<10 mg∙L^-1). Importantly, both CO₂ and N₂O emissions were minimized under mixotrophic conditions, indicating a high greenhouse gas mitigation potential. The obtained results indicate that one-stage H₂-driven mixotrophic N-assimilation process in hollow-fiber membrane bioreactors offers a viable solution for simultaneous wastewater treatment and resource recovery.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133398"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing anaerobic digestion of swine manure using magnetite: Insights into methane production and organic acids metabolism","authors":"Waris Khan ,&nbsp;Jung-Sup Lee ,&nbsp;Yeo-Myeong Yun","doi":"10.1016/j.biortech.2025.133397","DOIUrl":"10.1016/j.biortech.2025.133397","url":null,"abstract":"<div><div>This study evaluated the impact of increasing total ammonia nitrogen (TAN; 0–12,000  mg/L) representative of swine manure anaerobic digestion (AD), and the mitigating effect of magnetite supplementation. In control reactors, methane yield peaked at 164  mL/g COD at 750  mg TAN/L, then declined with higher TAN, while magnetite improved yields to 175 and 149  mL/g COD at 750 and 1,500  mg/L, respectively. The 30 % inhibitory concentration (IC₃₀) for free ammonia nitrogen (FAN) increased from 126 to 141  mg/L with magnetite, whereas IC₆₀ and IC₉₀ showed negligible changes, indicating limited mitigation at higher FAN levels. Specific methanogenic activity (SMA) tests demonstrated that magnetite-enhanced direct interspecies electron transfer (DIET) supported partial recovery of methane production from acetate and butyrate at 1,500  mg TAN/L, while lactate showed minimal improvement, and propionate remained strongly inhibited. Propionate accumulation confirmed its role as a metabolic bottleneck under ammonia stress. Overall, magnetite improved methanogenic activity under moderate TAN but was ineffective under elevated ammonia stress.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"439 ","pages":"Article 133397"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superior membrane fouling control in membrane bioreactors using reciprocation with limited aeration.","authors":"Runzhang Zuo, Canhui Song, Dajun Ren, Zihan Mei, Chuheng Xie, Feixiang Zan, Qian Li, Xiejuan Lu, Xiaohui Wu","doi":"10.1016/j.biortech.2025.133380","DOIUrl":"10.1016/j.biortech.2025.133380","url":null,"abstract":"<p><p>Membrane fouling control is crucial for the wide application of membrane bioreactors (MBR), highlighting the necessity for innovative strategies to reduce energy input and improve fouling control capability. In this study, three different membrane-fouling strategies, i.e., aeration, reciprocation, and reciprocation coupled with limited aeration (RecLA), were adopted and compared in MBR systems with a long-term investigation. Compared to the conventional aeration strategy, which achieved nitrogen and phosphorus removal efficiencies of 87.5 % ± 4.9 % and 30.2 % ± 4.3 % respectively, the reciprocation strategy demonstrated significantly higher removal efficiencies of 94.5 % ± 3.7 % for nitrogen and 94.3 % ± 3.7 % for phosphorus. More importantly, the filtration time was significantly extended from 4 days for aeration to 21.5 days for reciprocation and 26.7 days for RecLA. RecLA was effective in reducing cake layer thickness, enhancing foulant hydrophilicity, and decreasing the abundance of filamentous bacteria in the foulant. Particle image velocimetry analysis revealed that RecLA enhances bubble penetration into the module interior, increases the shear rate near the membrane surface, and mitigates foulant accumulation, thereby effectively alleviating membrane fouling. Therefore, the RecLA strategy achieves efficient membrane fouling control by enhancing hydrodynamic conditions and altering foulant properties, offering an innovative solution for the broader application of MBR systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133380"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent temporal causal inference framework for wastewater treatment plant nitrogen removal: Multi-stage spurious causal elimination","authors":"Zhichi Chen ,&nbsp;Qiang He ,&nbsp;Lianggen Ao ,&nbsp;Qingtao Zhang ,&nbsp;Cheng Cheng ,&nbsp;Fucheng Guo ,&nbsp;Anqi Xiao ,&nbsp;Jing Lv ,&nbsp;Xu Gao ,&nbsp;Hong Cheng","doi":"10.1016/j.biortech.2025.133394","DOIUrl":"10.1016/j.biortech.2025.133394","url":null,"abstract":"<div><div>Wastewater treatment operations require transparent, interpretable models for regulatory compliance and safety, yet the intricate mechanisms involved in biological nitrogen removal present significant challenges for achieving interpretable mechanistic understanding. To address this, this study proposes AquaCausal, a novel hybrid causal inference framework that integrates the time-aware Peter &amp; Clark Momentary Conditional Independence (PCMCI) algorithm, deep learning, and a multi-stage mechanism to eliminate spurious causal relationships. A perturbed simulation dataset was generated and validated using a calibrated wastewater treatment plant (WWTP) model, establishing a benchmark for causal discovery. The framework systematically refines causal relationships through L1-regularized Granger causality testing, permutation feature importance analysis, and a four-dimensional robustness assessment. This process reduced initial potential causal relationships by 73 %, ultimately identifying 25 core causal relationships with high confidence. The derived causal network quantified key time-lagged dependencies, establishing an interpretable, data-driven representation of nitrogen removal mechanisms for optimizing treatment processes and facilitating adaptive intelligent control.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"439 ","pages":"Article 133394"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Charge-specific impacts of polystyrene nanoplastics on acidogenesis and biofilm adaptation in Ethanoligenens harbinense","authors":"Jing Wang,&nbsp;Weimin Zhu,&nbsp;Xingze Zhang,&nbsp;Sitong Liu,&nbsp;Jun Ma,&nbsp;Defeng Xing","doi":"10.1016/j.biortech.2025.133389","DOIUrl":"10.1016/j.biortech.2025.133389","url":null,"abstract":"<div><div>Despite increasing awareness of the risks posed by nanoplastics (NPs) to environmental microbes, the charge-specific effects of functionalized NPs on anaerobic acidogenic bacteria remain poorly understood. This study investigated the impact of functionalized polystyrene (PS) NPs on <em>Ethanoligenens harbinense</em>, a model hydrogen-producing anaerobe. The growth, metabolic, and transcriptomic responses of this bacterium to non-functionalized (PS-NPs), amino-modified (PS-NH₂), and carboxyl-modified (PS-COOH) variants were examined. Compared with the control group without NPs addition, PS-NH₂ exerted the strongest inhibition, reducing hydrogen and ethanol production by 16 % and 20 %, respectively, while elevating reactive oxygen species (ROS) level by 148 %. It also decreased biomass and down-regulated the expression of ribosome- and translation-related genes. In parallel, biofilm adaptation resulted in an 12 % increase in polysaccharide. PS-COOH enhanced biofilm reinforcement with a 21 % increase in polysaccharides and up-regulation of <em>bapA</em> and membrane transporter-related genes. Overall, PS-NH₂ induced broad transcriptional changes, particularly in pathways related to the phosphotransferase system (PTS), ATP-binding cassette (ABC) transporters, genetic information processing, and signaling/regulatory systems in <em>E. harbinense</em>. These findings provide new insights into how surface charge modifications of NPs affect anaerobic bacterial metabolism and underscore their potential environmental risks.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"439 ","pages":"Article 133389"},"PeriodicalIF":9.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}