Bioresource Technology最新文献

{"title":"A high-yield xylanase platform for efficient xylooligosaccharides production from agricultural residues","authors":"Xin Shi,&nbsp;Tao Zhu,&nbsp;Yinghui Nian,&nbsp;Ruizhen Miao,&nbsp;Qirong Shen,&nbsp;Qun Wan","doi":"10.1016/j.biortech.2025.132919","DOIUrl":"10.1016/j.biortech.2025.132919","url":null,"abstract":"<div><div>Agricultural residues are promising lignocellulosic feedstocks for producing xylooligosaccharides (XOS) through xylanase-mediated enzymatic hydrolysis. Achieving high XOS yield with cost-effective pretreatment and minimal enzyme loading is essential in biorefinery. This study achieved high XOS yield through enzymatic hydrolysis of agricultural residues. A robust platform for xylanase production was established using genetically engineered <em>Pichia pastoris</em> to secrete thermostable xylanase (tXyn2 from <em>Trichoderma reesei</em>). <em>N</em>-glycosylation of tXyn2 reduced its adsorption strength to lignin by 43 %. Through expression cassette optimization and chaperone co-expression, the expression of tXyn2 was enhanced to 10.2 g/L in a 5-L bioreactor, representing the highest reported secretory expression level for <em>T. reesei</em> xylanase. The saccharification of alkaline-pretreated corncob and cottonseed hull with optimized enzyme loading resulted in the XOS yields of 65.3 % and 77.5 %, respectively. The enzyme cost of XOS production from corncob is as low as 50 USD/ton XOS. Membrane-purified XOS exhibited significant growth-promoting effects on cucumber seedlings. Foliar application of XOS improved photosynthetic efficiency in cucumber seedlings by 51.4 % and elevated the indole-3-acetic acid content of roots by 45.6 %, which enhanced shoot height by 32.9 % and root biomass by 60.3 %. This study provides a cost-effective solution for XOS production and expands its application in agriculture.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132919"},"PeriodicalIF":9.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of fermentative polyhydroxyalkanoate production from Cerbera odollam oil using Monod-based and multi-scale kinetic models","authors":"Sook Wei Lim ,&nbsp;Jibrail Kansedo ,&nbsp;Inn Shi Tan ,&nbsp;Jobrun Nandong ,&nbsp;Yie Hua Tan ,&nbsp;Man Kee Lam ,&nbsp;Clarence M. Ongkudon","doi":"10.1016/j.biortech.2025.132918","DOIUrl":"10.1016/j.biortech.2025.132918","url":null,"abstract":"<div><div>Two mathematical models were developed to elucidate the biosynthesis of polyhydroxyalkanoates (PHAs) by Pseudomonas resinovorans DSM 21078 from Cerbera odollam oil – (1) The modified Monod-based kinetic model was integrated with Andrews, Haldane, and Logistic kinetics to describe carbon, nitrogen, and self-inhibition effects on cell growth and PHA production. An additional nitrogen regulatory factor was incorporated to account for urease activity and alternative nitrogen sources during fermentation. (2) The multi-scale kinetic model was adapted to capture three dynamic modes in the biosynthesis process (i.e., using Triple First-Order model), namely fast and slow modes of PHA production, and PHA degradation mode. The fast factors could be prompted by the de novo fatty acid synthesis pathway, saturated fatty acids in the oil compounds, and late-exponential-phase cells in the culture. Overall, the established mathematical models offered a robust predictive framework, providing valuable insights into fermentation behaviour that could advance oil-based PHA production systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132918"},"PeriodicalIF":9.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis and empirical research on the effectiveness of biochar in remediating tetracyclines pollution in water bodies","authors":"Na Li ,&nbsp;Xiaochen Zhu ,&nbsp;Yahui Miao ,&nbsp;Zhenyao Wang ,&nbsp;Carol Sze Ki Lin ,&nbsp;Chong Li","doi":"10.1016/j.biortech.2025.132917","DOIUrl":"10.1016/j.biortech.2025.132917","url":null,"abstract":"<div><div>The rapid increase in tetracycline pollution from intensive aquaculture necessitates urgent action to reduce tetracycline levels in water bodies, as its stability promotes antibiotic resistance, disrupts aquatic ecosystems, and poses health risks to humans through bioaccumulation. Current treatment methods like ozone oxidation are often costly or ineffective. Biochar, recognised for its cost-effectiveness and adsorption capacity, provides a promising solution. Through a <em>meta</em>-analysis of 110 research articles integrating 537<!--> <!-->observations focused on plant-based biomass, this study identified the following: 1) key factors influencing biochar’s adsorption of tetracyclines include preparation processes and environmental conditions; 2)<!--> <!-->higher pyrolysis temperatures can boost adsorption capacity, especially above 600 °C; 3)<!--> <!-->pre-treatment using acids or salts is more effective than other modifiers. Additionally, a linear mixed-effects model predicted that specific pyrolysis temperatures combined with acid or salt modification would produce biochar with superior performance. Verification experiments with pine, poplar, and straw from wheat, rice, and corn modified with salts (CaCl₂, ZnCl₂, MgSO₄, AlCl₃, FeCl₃, K₂FeO₄) and acids (H₃PO₄, H₂SO₄, HNO₃) revealed significantly increased adsorption capacities, as predicted by the linear mixed-effects model. The adsorption capacity of biochar prepared from biomass modified with 1 M H₂SO₄ for 24 h and pyrolysed at 700 °C can reach 637.71 mg/g, and the biochar exhibits enhanced adsorption capacity compared with those previously reported. Results of this study demonstrate the feasibility and accuracy of using <em>meta</em>-analysis to predict the performance of biochar in the efficient adsorption of tetracycline from water and provide a robust scientific framework for customised biochar preparation with specific functions.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132917"},"PeriodicalIF":9.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and green extraction of chitin from mantis shrimp shell using deep eutectic solvents at room temperature","authors":"Jinjing Ma ,&nbsp;Deyu Chu ,&nbsp;Vokhidova Noyira Rakhimovna ,&nbsp;Peng Wang ,&nbsp;Shaomin Zhu ,&nbsp;Hongguo Xie ,&nbsp;Qishun Liu ,&nbsp;Heng Yin","doi":"10.1016/j.biortech.2025.132888","DOIUrl":"10.1016/j.biortech.2025.132888","url":null,"abstract":"<div><div>The exoskeleton of mantis shrimp (MS) is rich in chitin, making it an essential source of this biopolymer with potential applications. This study represents the first application of deep eutectic solvents (DESs) system, composed of lactic acid and triethylbenzylammonium chloride, for the extraction of chitin from mantis shrimp shells, achieving a balance between high-purity and high molecular weight (Mw) while ensuring solvent recyclability. Under optimized conditions, the extracted chitin achieved a purity of 95.65%, with the Mw approximately 2.5 times higher than that obtained using conventional acid-base methods. Remarkably, the chitin purity remained at 94.84% after three cycles of DESs. This study provides a new perspective for a green route of DESs for highly efficient extract of chitin from MS shells and addresses critical challenges in marine waste valorization.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132888"},"PeriodicalIF":9.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics reveals mechanistic impact of gut microbiota inhibition on lignocellulose biodegradation in Tenebrio molitor","authors":"Xinrui Mao ,&nbsp;Jiaming Li ,&nbsp;Wenbiao Jin ,&nbsp;Wei Han","doi":"10.1016/j.biortech.2025.132914","DOIUrl":"10.1016/j.biortech.2025.132914","url":null,"abstract":"<div><div><em>Tenebrio molitor</em> demonstrates an excellence ability to biodegrade lignocellulosic waste rapidly, though the specific mechanisms behind this process remain largely unknown. Among the eight types of antibiotics, levofloxacin had the most significant inhibitory effect on gut microorganisms of <em>Tenebrio molitor</em>. Following inhibition of gut microorganisms, notable reductions were observed in larval growth, triglyceride content, and lignocellulose degradation efficiency, suggesting a synergistic role between the gut tissue and gut microorganisms in lignocellulose biodegradation. Transcriptome and lipidome analysis revealed that the expression of lignocellulose genes partial compensatory response after the inhibition of gut microbes, and 41 lipids were specifically down-regulated. Co-expression networks revealed that triglyceride and phosphatidylethanolamine were significantly associated with the module, and the core regulatory genes within the module were glycerol-3-phosphate acyltransferase, phospholipase B and acetylcholinesterase. This study provides new insights into the effect and mechanism of gut microbiota inhibition of <em>Tenebrio molitor</em> on lignocellulose biodegradation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132914"},"PeriodicalIF":9.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrolysis of nitrogen-rich microalgae: kinetics, products, and amino acid contributions","authors":"Qixing Hu,&nbsp;Yibo Zhang,&nbsp;Chengyi Luo,&nbsp;Yuwei Mi,&nbsp;Mingming Chen,&nbsp;Yijie Zheng,&nbsp;Zhiquan Hu","doi":"10.1016/j.biortech.2025.132899","DOIUrl":"10.1016/j.biortech.2025.132899","url":null,"abstract":"<div><div>Pyrolysis offers a promising pathway for converting microalgae into high-value products. However, the governing mechanisms, particularly those involving amino acids, remain inadequately understood. Clarifying the behavior of amino acids during pyrolysis is essential for optimizing conversion efficiency and controlling pollution from <em>N</em>-containing products. This study investigates the pyrolysis of <em>N</em>-rich microalgae using three kinetic approaches, alongside product characterization. The model fitting quality is ranked as follows: the machine learning (ML) model (0.999), the model-free methods (0.981), and the independent parallel reaction model combined with particle swarm optimization algorithms (IPR-PSO) (0.910). With respect to actual components specificity, the ranking is reversed: IPR-PSO (actual components), model-free methods (pseudo-components), and ML model (component-independent). Compared with purely data-driven approaches, the IPR-PSO model provides mechanistic insights by associating reaction kinetics with specific amino acid contributions, identifying Leucine, Tyrosine, and Aspartate as key contributors with weights of 0.188, 0.149, and 0.081, respectively. Pyrolysis products were characterized by X-ray photoelectron spectroscopy (XPS), gas chromatography-mass spectrometry (GC-MS), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and a gas analyzer. Results indicate that as temperature increases, the protein-N in microalgae transforms into quaternary-N in biochar; pyridines, amides, unsaturated amide with carbon chain in bio-oil; and NH₃ in syngas—these products primarily originate from amino acid decomposition and reforming. This investigation elucidates the significant role that amino acids play in optimizing microalgae pyrolysis as a sustainable resource recovery technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132899"},"PeriodicalIF":9.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of Auxenochlorella protothecoides using tofu whey wastewater for aquaculture feed production: Integrating resource recovery and microalgal biomass valorization strategy","authors":"Liang Zhong,&nbsp;Xing Meng,&nbsp;Yulong Zhang,&nbsp;Zongfan Peng,&nbsp;Rong Zhou,&nbsp;Yufang Tang,&nbsp;Yuqin Li","doi":"10.1016/j.biortech.2025.132913","DOIUrl":"10.1016/j.biortech.2025.132913","url":null,"abstract":"<div><div>This study investigates the potential of utilizing tofu whey wastewater (TWW) for <em>Auxenochlorella protothecoides</em> cultivation and aquatic protein feed production. Results demonstrate that <em>A. protothecoides</em> cultured in TWW (AP-C-TWW) achieves biomass and protein yield of 16.45 g/L and 8.84 g/L, respectively, representing 78 % and 85 % increases compared to conventional medium. AP-C-TWW exhibits an essential-to-nonessential amino acid ratio of 0.7, fulfilling protein feed standards. Whilst TWW treatment with <em>A. protothecoides</em> results in the removal of 88 %, 96 %, and 99 % of total phosphorus, total nitrogen, and chemical oxygen demand, respectively, ensuring compliance with discharge regulations. Furthermore, replacing 5 % of dietary fishmeal with AP-C-TWW increases the final body weight and specific growth rate of <em>Oncorhynchus mykiss</em> by 10 % and 13 %, respectively, compared to control group. Additionally, it significantly enhances antioxidant capacity, innate immunity, and fillet quality. This study highlights the potential of TWW upcycling for sustainable microalgae-based aquaculture feed production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132913"},"PeriodicalIF":9.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of progesterone and norgestrel in wastewater by functional bacterial gels: Perspectives from simultaneous reduction of transformation products and endocrine disrupting effects","authors":"Xianda Hu ,&nbsp;Jinju Geng ,&nbsp;Ke Xu ,&nbsp;Qingmiao Yu ,&nbsp;Hongqiang Ren","doi":"10.1016/j.biortech.2025.132911","DOIUrl":"10.1016/j.biortech.2025.132911","url":null,"abstract":"<div><div>The steroidal progestogens have attracted much attention on their occurrence and fate in wastewater due to their endocrine disrupting effects to aquatic organisms at ng/L levels. Enhanced removal of progestogens by functional bacteria is an environmental-friendly and low-cost technology. However, the effectiveness of the immobilized functional bacteria in simultaneously removing target progestogens, their transformation products (TPs) and endocrine-disrupting risks in wastewater has not been well documented. This study isolated three strains of functional bacteria that can effectively degrade two typical progestogens, progesterone (<em>P</em>4) and norgestrel (NGT), immobilized the bacteria into bacterial gels and constructed the bacterial gels reactor (BGR). Batch experiment showed that the biodegradation of <em>P</em>4 and NGT by the bacterial gels followed first-order kinetics, with kinetic constants of 0.09 and 0.08 h⁻¹, respectively, and 11 and 12 TPs of <em>P</em>4 and NGT were identified by using high resolution mass spectrometry. The BGR was effective in removing <em>P</em>4 and NGT in long-time operating, with removal efficiencies of 99.3 % and 97.1 %, respectively. Further, the BGR was connected in series behind an A/A/O reactor. The long-time operating results showed that the BGR further enhanced <em>P</em>4 (62 %) and NGT (50 %) removal in the A/A/O secondary effluent, with simultaneously reducing the adverse effects of the effluent on transcripts of the zebrafish gonads. Additionally, relative abundances of most TPs were also reduced by the BGR. This study demonstrated the effectiveness of bacterial gels in simultaneously removing <em>P</em>4/NGT, TPs and endocrine disrupting risks in wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132911"},"PeriodicalIF":9.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient control of wastewater-derived dissolved organic nitrogen and its eutrophication potential via redox mediator at low temperatures: Molecular and microbial mechanism","authors":"Huazai Cheng,&nbsp;Jiaqian You,&nbsp;Kewei Liao,&nbsp;Hongqiang Ren,&nbsp;Haidong Hu","doi":"10.1016/j.biortech.2025.132910","DOIUrl":"10.1016/j.biortech.2025.132910","url":null,"abstract":"<div><div>With increasingly stringent nitrogen discharge standards, effluent dissolved organic nitrogen (DON) in wastewater treatment plants has been recognized as a more significant contributor to the eutrophication of receiving waters than inorganic nitrogen. However, reducing wastewater-derived DON and its eutrophication potential under low-temperature conditions remains challenging. Here, we report a strategy to reduce effluent DON concentration and its labile components of post-denitrification bioreactors at low temperatures by adding small amounts (i.e., 0.1 and 0.15 mM) of 2-hydroxy-1,4-naphoquinone (HNQ). The effluent DON concentration from the bioreactors upon HNQ addition was lower than that in control bioreactors, with a maximum reduction of 64 %. Ultrahigh-resolution mass spectrometry results showed that HNQ addition reduced labile DON, including lipids-, proteins/amino sugars-, and carbohydrates-like molecules, thereby controlling the DON eutrophication potential, which was confirmed using a subsequent algal bioassay. Moreover, partial least-squares path modeling analysis showed that HNQ dosing significantly altered the microbial community (<em>β</em> =  − 0.95, <em>p</em> &lt; 0.01) and function (<em>β</em> =  − 0.97, <em>p</em> &lt; 0.01). This in turn influenced the production and consumption of labile DON, ultimately reducing the DON eutrophication potential. This study demonstrates a simple in situ upgrading scheme for existing post-denitrification devices to address DON-related eutrophication crises occurring in cold weather.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132910"},"PeriodicalIF":9.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of new sulfamethoxazole catabolic pathways in whole-cell catalyst of bacterium Kocuria rhizophila SA117","authors":"Tomáš Řezanka ,&nbsp;Jiří Zahradník ,&nbsp;Sofía G. Zavala-Meneses ,&nbsp;Helena Marešová ,&nbsp;Michal Řezanka ,&nbsp;Helena Pelantová ,&nbsp;Michal Grulich ,&nbsp;Václav Filištein ,&nbsp;Andrea Palyzová","doi":"10.1016/j.biortech.2025.132912","DOIUrl":"10.1016/j.biortech.2025.132912","url":null,"abstract":"<div><div>Sulfamethoxazole (SMX) and its residues exhibit high environmental persistence due to their resistance to conventional degradation processes. The bacterial strain <em>Kocuria rhizophila</em> SA117, isolated from polluted soils, was characterized biochemically, phylogenetically, and −omically. Herein, we describe a complete degradation pathway for SMX and determine two putative pathways: cleavage of the benzene ring and the degradation of the substituted isoxazole, leading to the formation of non-toxic Krebs cycle metabolites. Based on molecular structures containing ¹³C₆-labeled carbons and ²H₃ atoms, thirty metabolites were identified by high-resolution tandem mass spectrometry. Genomic and proteomic analysis of strain SA117 revealed its ability to perform a wide range of metabolic activities under sulfamethoxazole selective pressure. These activities include energy and sulfur metabolism, adaptation to stress conditions, and catabolism of aromatic compounds. This study has greatly enhanced the understanding of microbial sulfonamide degradation and highlighted the potential of the bacterium <em>Kocuria</em> in remediation strategies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132912"},"PeriodicalIF":9.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}