Bioresource Technology最新文献_第10页

Sustainable lipid production by oleaginous yeasts: Current outlook and challenges

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132205

Kukkala Kiran Kumar , Farha Deeba , Ajay Kumar Pandey , Asimul Islam , Debarati Paul , Naseem A. Gaur

{"title":"Sustainable lipid production by oleaginous yeasts: Current outlook and challenges","authors":"Kukkala Kiran Kumar , Farha Deeba , Ajay Kumar Pandey , Asimul Islam , Debarati Paul , Naseem A. Gaur","doi":"10.1016/j.biortech.2025.132205","DOIUrl":"10.1016/j.biortech.2025.132205","url":null,"abstract":"<div><div>Yeast lipid has gained prominence as a sustainable energy source and so various oleaginous yeasts are being investigated to create efficient lipogenic platforms. This review aims to assess the various biotechnological strategies for enhanced production of yeast lipids via agro-waste processing and media engineering including multiomic analyses, genetic engineering, random mutagenesis, and laboratory adaptive evolution. The review also emphasizes the role of cutting-edge omics technologies in pinpointing differentially expressed genes and enriched networks crucial for designing advanced metabolic engineering strategies for prominent oleaginous yeast species. The review addresses the challenges and future prospects of a viable lipid production industry that is possible through advancements in current technologies, strain improvement, media optimization and techno-economic and life cycle analyses at lab, pilot and industrial scales. This review comprehensively provides deep insights for enhancement of yeast lipid biosynthesis to reach industrially benchmarked standard of a lipid production platform.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132205"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing near-infrared and Raman spectral sensing and artificial intelligence for real-time monitoring and precision control of bioprocess

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132204

Feng Xu , Lihuan Su , Hao Gao , Yuan Wang , Rong Ben , Kaihao Hu , Ali Mohsin , Chao Li , Ju Chu , Xiwei Tian

{"title":"Harnessing near-infrared and Raman spectral sensing and artificial intelligence for real-time monitoring and precision control of bioprocess","authors":"Feng Xu , Lihuan Su , Hao Gao , Yuan Wang , Rong Ben , Kaihao Hu , Ali Mohsin , Chao Li , Ju Chu , Xiwei Tian","doi":"10.1016/j.biortech.2025.132204","DOIUrl":"10.1016/j.biortech.2025.132204","url":null,"abstract":"<div><div>Effective monitoring and control of bioprocesses are critical for industrial biomanufacturing. This study demonstrates the integration of near-infrared and Raman spectroscopy for real-time monitoring and precise control of gentamicin fermentation. The orthogonal method reduced redundant features and improved spectral model performance by 9.2–100.4 % in terms of the coefficient of determination (R<sup>2</sup>). The combinatorial spectral model outperformed single-source models in external validation (R<sup>2</sup> > 0.99). An AI-based platform, combining dual-sensors data collection, ML-based prediction, and automated feeding control, was developed for fully automated fed-batch fermentation. This platform dynamically adjusted feeding rates, maintained low glucose concentrations (5 g/L) with accuracy and coefficient of variation below 2 %, and increased gentamicin C1a concentration (346.5 mg/L) by 33.0 % compared to traditional intermittent feeding. These findings underscore the transformative potential of combinatorial spectroscopy and machine learning for real-time bioprocess monitoring, offering a scalable solution for enhancing industrial fermentation efficiency and product titer.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132204"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143369688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient bio-oxidation of biomass-derived furan-2,5-dicarbaldehyde to 5-formyl-2-furoic acid and 2,5-furandicarboxylic acid via whole-cell biocatalysis

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132201

Qizhen Yang , Changqing Wu , Tingting Zhang , Yu-Cai He , Cuiluan Ma

{"title":"Efficient bio-oxidation of biomass-derived furan-2,5-dicarbaldehyde to 5-formyl-2-furoic acid and 2,5-furandicarboxylic acid via whole-cell biocatalysis","authors":"Qizhen Yang , Changqing Wu , Tingting Zhang , Yu-Cai He , Cuiluan Ma","doi":"10.1016/j.biortech.2025.132201","DOIUrl":"10.1016/j.biortech.2025.132201","url":null,"abstract":"<div><div>The production of bio-based fine chemicals is increasingly important to address fossil energy shortages, climate change, and other environmental issues. Using abundant and renewable bioresource as starting material to manufacture bio-based fine chemicals will achieve a green circular economy. 5-Formyl-2-furoic acid (FFCA) and 2,5-furandicarboxylic acid (FDCA) have broad application prospects in fuels, chemical intermediates, polymers and pharmaceuticals. In this research, a green and effectual biotransformation process was built to manufacture FFCA and FDCA from biomass-derived furan-2,5-dicarbaldehyde (DFF) in DMSO-H<sub>2</sub>O using recombinant <em>Escherichia coli</em> cells carrying AAOase (aryl-alcohol oxidase) as biocatalyst. Under mild performance conditions, FFCA could be produced from 75 mM DFF in a high yield (92.3 %) within 24 h. 25 mM DFF was fully oxidized to FDCA within 24 h. The research established an effectual biocatalytic system for transforming HMF-derived DFF with AAOase biocatalysts into valuable biomass-derived products. This study holds great promising for sustainably synthesizing FFCA and FDCA.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132201"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy metabolism coordination for the byproduct-free biosynthesis of 1,3-propanediol in Escherichia coli

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132147

Junhak Lee , Tayyab Islam , Seunghyun Cho , Nandakumar Arumugam , Vivek Kumar Gaur , Sunghoon Park

{"title":"Energy metabolism coordination for the byproduct-free biosynthesis of 1,3-propanediol in Escherichia coli","authors":"Junhak Lee , Tayyab Islam , Seunghyun Cho , Nandakumar Arumugam , Vivek Kumar Gaur , Sunghoon Park","doi":"10.1016/j.biortech.2025.132147","DOIUrl":"10.1016/j.biortech.2025.132147","url":null,"abstract":"<div><div>The efficient, byproduct-free production of 1,3-propanediol (1,3-PDO), a valuable chemical widely used in various industries, presents a significant challenge in bio-based manufacturing, due to its reduced nature. In this study, <em>Escherichia coli</em> K12 was engineered to achieve high-yield 1,3-PDO production by optimizing glucose metabolism and utilizing glycerol as a feedstock. Glycolytic flux was rerouted to the NADPH-generating pentose phosphate (PP) pathway, linking NADPH regeneration to 1,3-PDO biosynthesis. These modifications enhanced carbon utilization and eliminated byproduct formation. The engineered strain, PK19-D1Q1, achieved a record 1,3-PDO titer of 1.06 mol/L, with glycerol and glucose yields of 0.99 mol/mol and 2.01 mol/mol, respectively, in fed-batch fermentation. Furthermore, the strain’s ability to maintain high productivity with crude glycerol underscores its potential for industrial-scale applications using low-cost, sustainable substrates. This study sets a benchmark for scalable, sustainable 1,3-PDO production, showcasing the integration of cofactor balancing and pathway engineering for bio-based chemical manufacturing.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132147"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secretory and metabolic engineering of squalene in Yarrowia lipolytica

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132171

Liang Chai , Jiaxin Che , Xiaoxu Liu, Zhengdong Wang, Qingsheng Qi, Jin Hou

{"title":"Secretory and metabolic engineering of squalene in Yarrowia lipolytica","authors":"Liang Chai , Jiaxin Che , Xiaoxu Liu, Zhengdong Wang, Qingsheng Qi, Jin Hou","doi":"10.1016/j.biortech.2025.132171","DOIUrl":"10.1016/j.biortech.2025.132171","url":null,"abstract":"<div><div>This short communication systematically explored ATP-binding cassette (ABC) transporters and developed a carrier protein-mediated metabolite trafficking system to engineer <em>Yarrowia lipolytica</em> for secretion of squalene. Specially, the squalene biosynthesis pathway was overexpressed to improve squalene production. Subsequently, a series of ABC transporters and oxysterol-binding proteins (OSH) were screened, with the combination of <em>SNQ2</em> and <em>OSH3</em> showing the most significant improvement in squalene secretion. Additionally, we designed a carrier protein-mediated metabolite trafficking system by fusing the binding domain of OSH3 with a secretion signal peptide. This approach, combined with the optimization of signal peptides, increased squalene secretion to 144.67 mg/L, which represented a 17-fold improvement over the initial strain. This system also demonstrated versatility by facilitating the extracellular export of β-carotene. In fed-batch cultivation, the engineered strain secreted 3.43 g/L of squalene extracellularly, accounting for 27.2 % of the total production. It represented the highest secretion level reported to date. This study establishes an efficient secretion platform for the transport of membrane-impermeable squalene in <em>Y. lipolytica</em>, providing valuable insights and strategies for the microbial production of terpenoids.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132171"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Fe/Mg-modified lignocellulosic biochar on in vitro ruminal microorganism fermentation of corn stover

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132172

Yuping Liu , Jiyu Sun , Taotao Zhao , Lin Wang , Chenyu Zhao , Jingjing Fu , Dawei Li , Haiye Yu

{"title":"Effects of Fe/Mg-modified lignocellulosic biochar on in vitro ruminal microorganism fermentation of corn stover","authors":"Yuping Liu , Jiyu Sun , Taotao Zhao , Lin Wang , Chenyu Zhao , Jingjing Fu , Dawei Li , Haiye Yu","doi":"10.1016/j.biortech.2025.132172","DOIUrl":"10.1016/j.biortech.2025.132172","url":null,"abstract":"<div><div>This study investigated the effectiveness and synergistic mechanism of trace-element-modified biochar (BC) on <em>in vitro</em> ruminal fermentation of lignocellulose. Fe/Mg-modified BC containing Fe@BC, Mg@BC and Fe/Mg@BC were prepared, and their effects on <em>in vitro</em> ruminal fermentation of corn stover were assessed. Results indicate that Mg@BC achieved the highest reducing-sugar content (320.4 mg/L) with an additive dose of 12 g/L and a substrate load of 4 %, owing to the presence of enriched lignocellulolytic microorganisms like Treponema and Bacillus. Moreover, Mg@BC promoted the growth of acid-producing bacteria, including Bacteroides and Lachnospiraceae, resulting in the highest production of volatile fatty acid (3.2 g/L). Fe@BC increased the amount of hydrogenogens including Prevotellaceae_YAB2003 and Lachnospiraceae_NK3A20, contributing to the highest hydrogen production. Meanwhile, Fe/Mg@BC facilitated the growth of Succiniclasticum and Lactobacillus, which effectively produce succinic and lactic acids. These findings provide new insights into efficient lignocellulose bioconversion via <em>in vitro</em> ruminal fermentation with Fe/Mg-modified BC supplementation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132172"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Artificial cell-free system for the sustainable production of acetoin from bioethanol” [Bioresour. Technol. 419 (2025) 132059]

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-06 DOI: 10.1016/j.biortech.2025.132141

David Muñoz-Sánchez, Albert Carceller, Gregorio Álvaro, Óscar Romero, Marina Guillén

引用次数: 0

Simultaneous removal of ammonia, cadmium, and oxytetracycline via a double-layer immobilized bioreactor driven by manganese redox: Optimization and potential mechanism

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-06 DOI: 10.1016/j.biortech.2025.132150

Jingting Feng , Meng Cao , Yue Wang , Liang Xu , Yihan Bai , Wenjing Cheng , Junfeng Su

{"title":"Simultaneous removal of ammonia, cadmium, and oxytetracycline via a double-layer immobilized bioreactor driven by manganese redox: Optimization and potential mechanism","authors":"Jingting Feng , Meng Cao , Yue Wang , Liang Xu , Yihan Bai , Wenjing Cheng , Junfeng Su","doi":"10.1016/j.biortech.2025.132150","DOIUrl":"10.1016/j.biortech.2025.132150","url":null,"abstract":"<div><div>The coexistence of ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N), heavy metals and antibiotics in composite polluted wastewater has garnered significant attention. This study developed a novel double-layer biological carrier using sodium alginate, diatomite, polyvinyl alcohol, manganese-modified biochar, and pyrolusite, loaded with strains YZ8 and MA23 to form an efficient bioreactor (M1). Under conditions of a hydraulic retention time of 24 h, the carbon to nitrogen ratio and pH were 1.5 and 6.5, M1 achieved an average NH<sub>4</sub><sup>+</sup>-N removal efficiency of 99 %. Additionally, the average removal efficiencies of cadmium and oxytetracycline by M1 through biosorption, co-precipitation and Mn(Ⅲ) oxidation reached 90 % and 85 %, respectively. High-throughput results indicated that M1 had a relatively high abundance of functional bacterial genera. Comparative KEGG analysis revealed that M1 promoted the expression of functional genes involved in N cycling and Mn transformation. This study offers new perspectives on tackling the issue of composite water environmental pollution.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132150"},"PeriodicalIF":9.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of bacterial cell concentration on microbial-mediated cerium carbonate precipitation for efficient heavy metal removal: Insights from adsorption isotherm, kinetics, and thermodynamics

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-06 DOI: 10.1016/j.biortech.2025.132151

Md. Taharia , Koyeli Das , Uttara Sukul , Hung-Chun Chao , Pritam Banerjee , Gobinda Dey , Raju Kumar Sharma , Pin-Yun Lin , Tung-Che Hung , Chien-Yen Chen

{"title":"Impact of bacterial cell concentration on microbial-mediated cerium carbonate precipitation for efficient heavy metal removal: Insights from adsorption isotherm, kinetics, and thermodynamics","authors":"Md. Taharia , Koyeli Das , Uttara Sukul , Hung-Chun Chao , Pritam Banerjee , Gobinda Dey , Raju Kumar Sharma , Pin-Yun Lin , Tung-Che Hung , Chien-Yen Chen","doi":"10.1016/j.biortech.2025.132151","DOIUrl":"10.1016/j.biortech.2025.132151","url":null,"abstract":"<div><div>Heavy metals (HMs) pollution is a pervasive environmental issue needs significant attention through bioremediation. Present study investigated the potentiality of Microbial-mediated Cerium Carbonate Precipitation (MMCCP) in simultaneous removal of HMs (Cr, Pb, and Cu) using different cellular concentration of <em>Sporosarcina pasteurii</em>. Results from SEM analysis revealed formation of spherical and rod-like structures (∼112nm), and finally XRD and FTIR confirmed the formation of high-purity crystalline <span><math><mrow><msub><mrow><mi>CeCO</mi></mrow><mrow><mn>3</mn></mrow></msub><mi>OH</mi></mrow></math></span> with surface-bound hydroxyl groups and <span><math><mrow><msubsup><mrow><mi>CO</mi></mrow><mrow><mn>3</mn></mrow><mrow><mn>2</mn><mo>-</mo></mrow></msubsup></mrow></math></span> ions, indicating successful cerium incorporation and formation in <span><math><mrow><msub><mrow><mi>CeCO</mi></mrow><mrow><mn>3</mn></mrow></msub><mi>OH</mi></mrow></math></span>. Moreover, optimal removal efficiencies for Cr (99%), Pb (99%), and Cu (68%) were achieved within 80min at 6mg/L concentration and 0.05g adsorbent dose. Adsorption followed the Langmuir isotherm (R<sup>2</sup> = 0.9) and pseudo-second-order kinetics, with thermodynamic parameters indicating spontaneity and exothermicity (ΔG < 0, ΔH < 0, ΔS > 0). These findings demonstrate MMCCP’s potential as an effective and sustainable approach for HMs remediation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132151"},"PeriodicalIF":9.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed valorization as anaerobic co-substrate with fat, oil, and grease: Biomethane potential and microbial dynamics

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-05 DOI: 10.1016/j.biortech.2025.132155

Shah Faisal , Adel W. Almutairi , Irfan Saif , Li Ting , Qingyuan Wang , Ahmad Mustafa , Reham Ebaid

{"title":"Seaweed valorization as anaerobic co-substrate with fat, oil, and grease: Biomethane potential and microbial dynamics","authors":"Shah Faisal , Adel W. Almutairi , Irfan Saif , Li Ting , Qingyuan Wang , Ahmad Mustafa , Reham Ebaid","doi":"10.1016/j.biortech.2025.132155","DOIUrl":"10.1016/j.biortech.2025.132155","url":null,"abstract":"<div><div>The present study explored the anaerobic co-digestion (AcD) of seaweed <em>Gracilaria vermiculophylla</em> with fat, oil, and grease (FOG) at 75, 50, and 25 % <em>w/w</em> of volatile solids (VS). Mono-digestion of FOG and SW led to a methane production of 133 and 109 mL/(g.d) with 40 days lag-phase, lower than 235 mL/(g.d) of AcD at FOG-50:SW-50 with reduced lag-phase of 20 days. The palmitic and oleic acid reduction was 95 % in the reactors FOG-50:SW-50, followed by FOG-25:SW-75, which was 84 %, as compared to FOG mono-digestion (47 %). Relative abundance of <em>Firmicutes</em>, <em>Chloroflexi</em>, and <em>Bacteroidetes</em> were enriched during AcD. The relative abundance of <em>Methanosaeta</em> was enhanced (40–90 %) in FOG-50:SW-50 compared with FOG-100:SW-0 as the reduction in <em>Methanosaeta</em> was replaced by <em>Methanoculleus</em> (30 %) and <em>RuMen-M2</em> (10 %). The present study offers essential perspectives for the AcD of FOG with SW, showcasing the benefits of SW as a co-substrate for improved methane recovery from FOG.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132155"},"PeriodicalIF":9.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0