Bioresource Technology最新文献_第10页

Insights into the impact of feeding with polymers on aerobic granular sludge development and stability: Performance and mechanisms

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-07 DOI: 10.1016/j.biortech.2025.132368

Mingyue Geng , Ting Li , Fangshu Qu , Shanshan Gao , Jiayu Tian

{"title":"Insights into the impact of feeding with polymers on aerobic granular sludge development and stability: Performance and mechanisms","authors":"Mingyue Geng , Ting Li , Fangshu Qu , Shanshan Gao , Jiayu Tian","doi":"10.1016/j.biortech.2025.132368","DOIUrl":"10.1016/j.biortech.2025.132368","url":null,"abstract":"<div><div>In this study, the effect of feeding with polymers on aerobic granular sludge (AGS) formation and stability was comprehensively investigated during 235-day operation. Results showed that the granules developed in starch-fed reactor possessed fluffy surface with overgrowth of granule size, and 60 % flocs were produced in protein-fed reactor, identifying feeding with polymers deteriorated AGS development and stability. Moreover, substrate conversion analysis revealed that ∼ 14 % of the consumed COD was recovered as storage of poly-hydroxybutyrate in polymer-fed reactor, much lower than 63.7 % in acetate-fed reactor. Extended Derjaguin-Landau-Verwey-Overbeek theory analysis showed that feeding with polymers increased the cell–cell energy barriers to 307.8 ∼ 388.8 kT, weakening the microbial aggregation capacity in AGS system. Microbial population results found that the relative abundance of <em>Candidatus_Competibacter</em> in protein- and starch-fed reactor displayed 0.01 ∼ 6.1 % and 0.07 ∼ 3.7 %, much lower than 81 % in acetate-fed reactor. Assembly mechanism analysis demonstrated that feeding with polymers enhanced the stochastic selection in shaping microbial assembly.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132368"},"PeriodicalIF":9.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient immobilization of cellulase on polydopamine-modified nickel foam for enhanced lignocellulose conversion

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-06 DOI: 10.1016/j.biortech.2025.132361

Shi Wang , Bo Zhao , Shengxian Cao , Gong Wang , Zheng Dong , Zheng Yang , Xinglin Niu , Qinglong Zhao , Yongli Wang , Yue Ma

{"title":"Efficient immobilization of cellulase on polydopamine-modified nickel foam for enhanced lignocellulose conversion","authors":"Shi Wang , Bo Zhao , Shengxian Cao , Gong Wang , Zheng Dong , Zheng Yang , Xinglin Niu , Qinglong Zhao , Yongli Wang , Yue Ma","doi":"10.1016/j.biortech.2025.132361","DOIUrl":"10.1016/j.biortech.2025.132361","url":null,"abstract":"<div><div>In situ saccharification of lignocellulose is essential for efficiently converting biomass into value-added products. In this study, nickel foam underwent electrochemical pore size regulation and polydopamine modification to produce polydopamine-modified foam nickel with aperture control (PNiF-AC), which was used for cellulase immobilization and in situ saccharification of wheat straw. PNiF-AC demonstrated excellent hydrophilicity, biocompatibility, and robust adsorption–desorption cycling properties. Immobilizing cellulase onto PNiF-AC significantly improved its thermal stability by 64.8 %. The immobilized cellulase yielded 17.0 mg/mL of reducing sugars during the in situ saccharification of wheat straw, representing a 20 % increase over free cellulase. Moreover, after eight cycles of magnetic stirring, the reducing sugar yield decreased by only 19.4 %, highlighting the remarkable reusability and stability of the immobilized cellulase. These findings establish polydopamine-modified foam nickel as an efficient platform for cellulase immobilization, presenting a novel approach to improving lignocellulose conversion processes.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132361"},"PeriodicalIF":9.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas production

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-05 DOI: 10.1016/j.biortech.2025.132353

Nasreen Nasar , Giulia Pizzagalli , Frederic Coulon , Yadira Bajón-Fernández

{"title":"Enzymes targeting distinct hydrolysis blind-spots of thermal and biological pre-treatments significantly uplift biogas production","authors":"Nasreen Nasar , Giulia Pizzagalli , Frederic Coulon , Yadira Bajón-Fernández","doi":"10.1016/j.biortech.2025.132353","DOIUrl":"10.1016/j.biortech.2025.132353","url":null,"abstract":"<div><div>Thermal hydrolysis process (THP) and biological hydrolysis (BH) are key pre-treatment technologies for anaerobic digestion (AD), termed advanced anaerobic digesters (AADs). They target the rate-limiting hydrolysis step in AD. This study evaluates full-scale pre-treatments for macromolecule bias and the implementation of hydrolysis enzymes to enhance biogas yield. Findings show THP significantly improves protein and carbohydrate solubilisation by 30% and 25%, respectively, but fully hydrolyses only carbohydrates. In contrast, BH targets fibres and proteins, achieving 35% and 23% solubilisation, and only partially hydrolyses carbohydrates. Biomethane potential (BMP) tests indicate that protease enzymes raise biomethane yield by 20–30% for AAD with THP pre-treatment. In comparison, α-amylase increases it by over 30% for AAD with BH pre-treatment. This study tailors enzyme selection and dosage to specifically address the unique “hydrolysis blind spot” of each pre-treatment, providing a strategic framework to enhance AD technologies by an improved understanding of macromolecule selectivity and their transformation pathways.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132353"},"PeriodicalIF":9.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic reprogramming and machine learning-guided cofactor engineering to boost nicotinamide mononucleotide production in Escherichia coli

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-05 DOI: 10.1016/j.biortech.2025.132350

Bo Xiong , Tianrui Yang , Zixiong Zhang , Xiang Li , Huan Yu , Lian Wang , Zixuan You , Wenbin Peng , Luyu Jin , Hao Song

{"title":"Metabolic reprogramming and machine learning-guided cofactor engineering to boost nicotinamide mononucleotide production in Escherichia coli","authors":"Bo Xiong , Tianrui Yang , Zixiong Zhang , Xiang Li , Huan Yu , Lian Wang , Zixuan You , Wenbin Peng , Luyu Jin , Hao Song","doi":"10.1016/j.biortech.2025.132350","DOIUrl":"10.1016/j.biortech.2025.132350","url":null,"abstract":"<div><div>Nicotinamide mononucleotide (NMN) is a bioactive compound in NAD(P)<sup>+</sup> metabolism, which exhibits diverse pharmaceutical interests. However, enhancing NMN biosynthesis faces the challange of competing with cell growth and disturbing intracellular redox homeostasis. Herein, we boosted NMN production in <em>Escherichia coli</em> by reprogramming central carbon metabolism with a machine learning (ML)-guided cofactor engineering strategy. Engnieering NMN biosynthesis-related pathway directed carbon flux toward NMN with the NADPH level increased by 73 %, which, although enhanced NMN titer (2.45 g/L), impaired cell growth. A quorum sensing (QS)-controlled cofactor engineering system was thus contructed and optimized by ML models to address redox imbalance, which led to 3.04 g/L NMN with improved cell growth. The final strain S344 produced 20.13 g/L NMN in fed-batch fermentation. This study showed that perturbation on cofactor level is a crucial limiting factor for NMN biosynthesis, and proposed a novel ML-guided strategy to manipulate intracellular redox state for efficient NMN production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132350"},"PeriodicalIF":9.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subtleties of tetracycline removal during growth of microalgae-fungi consortia: Mechanistic insights from perspectives of extra- and intracellular metabolites

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-05 DOI: 10.1016/j.biortech.2025.132352

Zeyuan Wang , Yaqian Yuan , Yan Shi , Yu Hong

{"title":"Subtleties of tetracycline removal during growth of microalgae-fungi consortia: Mechanistic insights from perspectives of extra- and intracellular metabolites","authors":"Zeyuan Wang , Yaqian Yuan , Yan Shi , Yu Hong","doi":"10.1016/j.biortech.2025.132352","DOIUrl":"10.1016/j.biortech.2025.132352","url":null,"abstract":"<div><div>This study focused on tetracycline (TC) as the target antibiotic and utilized the emerging microbial system microalgae-fungi consortia to treat it. Results indicate that consortia composed of microalgae <em>Chlorella</em> sp. HL and fungi HW12 (<em>Aspergillus caespitosus</em>) (HL-HW12) exhibited the optimum TC removal (93.00 %, residual concentration: 2.73 mg/L) and biomass harvesting efficiency (92.69 %) among the five kinds of constructed microalgae-fungi consortia. Mechanism analysis indicated that outside the cell, microalgae-fungi consortia strengthened TC removal and biomass harvesting by augmenting the contents of proteins, polysaccharides, fulvic acids, and humic acids. While within the cell, microalgae-fungi consortia adjusted the abundance of critical metabolites in the amino acid metabolism, nucleotide metabolism, and other metabolic pathways to cope with the coercion of TC and facilitated its elimination. This study not only provides good TC microbial treatment systems but also comprehensively reveals the TC removal and metabolic response mechanisms by microalgae-fungi consortia.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132352"},"PeriodicalIF":9.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar mediated differential regulation of oxidative stress and energy supply in Bacillus subtilis and Rhizoctonia solani

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-05 DOI: 10.1016/j.biortech.2025.132317

Zihe Deng , Jianwen Wang , Jonathan A. Bennett , Wenjun Shao , Ziyuan An , Yanhui He , Fei Tian , Zhansheng Wu

{"title":"Biochar mediated differential regulation of oxidative stress and energy supply in Bacillus subtilis and Rhizoctonia solani","authors":"Zihe Deng , Jianwen Wang , Jonathan A. Bennett , Wenjun Shao , Ziyuan An , Yanhui He , Fei Tian , Zhansheng Wu","doi":"10.1016/j.biortech.2025.132317","DOIUrl":"10.1016/j.biortech.2025.132317","url":null,"abstract":"<div><div>Biochar (BC) significantly influences microbial metabolism, but its contrasting effects on different microorganisms remain unclear. This research explores the distinct regulatory mechanisms of BC on <em>B. subtilis</em> and <em>R. solani</em>. BC, consisting of micro-BC and nano-BC, generates reactive oxygen species (ROS), causing oxidative stress. Nano-BC can penetrate cells, leading to damage. In <em>B. subtilis</em>, BC initially inhibits growth, triggering endospore formation to expel nano-BC. <em>B. subtilis</em> secreted extracellular polymeric substances (EPS), which aggregated nano-BC, enhanced cell adhesion, and reduced intracellular ROS (from 2.0 to 1.5-fold), promoting growth later with BC’s nutrient support. Conversely, <em>R. solani</em> cannot block nano-BC entry, activating mitophagy and suppressing genes like <em>ATP1,2</em> involved in oxidative phosphorylation and tricarboxylic acid cycle. This results in ATP deficiency, collapses antioxidant system, raises ROS (from 3.9 to 4.5-fold), decreases cell survival, and leads to cell death. These findings highlight BC’s selective microbial regulation and its potential for safe agricultural and environmental use.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132317"},"PeriodicalIF":9.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Static magnetic field-enhanced cathodic electrocatalysis of Fe3O4-based nitrogen-doped carbon for improving the performance of microbial fuel cells

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-04 DOI: 10.1016/j.biortech.2025.132345

Hai-Xia Liao, Dong-Ni Ou, Xiao-Feng Zhou, Ying-Qi Ouyang, Hui-Huan Jiang, Nan Li, Zhao-Qing Liu

{"title":"Static magnetic field-enhanced cathodic electrocatalysis of Fe3O4-based nitrogen-doped carbon for improving the performance of microbial fuel cells","authors":"Hai-Xia Liao, Dong-Ni Ou, Xiao-Feng Zhou, Ying-Qi Ouyang, Hui-Huan Jiang, Nan Li, Zhao-Qing Liu","doi":"10.1016/j.biortech.2025.132345","DOIUrl":"10.1016/j.biortech.2025.132345","url":null,"abstract":"<div><div>Enhancing oxygen reduction reaction (ORR) electrocatalysis through an external static magnetic field to improve the performance of microbial fuel cells (MFCs) is technically feasible, but its application in MFCs remains largely unexplored. Herein, we present a Fe<sub>3</sub>O<sub>4</sub>-based nitrogen-doped carbon (Fe<sub>3</sub>O<sub>4</sub>@N<img>C<img>2) magnetic catalyst that significantly boosts ORR catalytic activity, increasing the half-wave potential (E<sub>1/2</sub>) of the ORR by approximately 20 mV with a magnetic field strength of 140 mT. When the Fe<sub>3</sub>O<sub>4</sub>@N<img>C<img>2 cathode is combined with an external magnetic field into the MFCs, the maximum power density of the MFC can reach 553.17 ± 7.16 mW m<sup>−2</sup>. This performance notably exceeds that of the same MFCs operated without a magnetic field (522.26 ± 4.25 m<sup>−2</sup>) and that of MFCs equipped with a Pt/C cathode (447.29 ± 2.16 mW m<sup>−2</sup>). This study introduces an effective and straightforward cathodic magnetic enhancement approach, offering promising avenues for advancing MFCs technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132345"},"PeriodicalIF":9.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of indigenous vs. cultivated microalgae strains on biomass accumulation, microbial community composition, and nutrient removal in algae-based dairy wastewater treatment

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-04 DOI: 10.1016/j.biortech.2025.132349

KhinKhin Phyu , Suli Zhi , David W. Graham , Yuang Cao , Xiaoyu Xu , Jiahua Liu , Han Wang , Keqiang Zhang

{"title":"Impact of indigenous vs. cultivated microalgae strains on biomass accumulation, microbial community composition, and nutrient removal in algae-based dairy wastewater treatment","authors":"KhinKhin Phyu , Suli Zhi , David W. Graham , Yuang Cao , Xiaoyu Xu , Jiahua Liu , Han Wang , Keqiang Zhang","doi":"10.1016/j.biortech.2025.132349","DOIUrl":"10.1016/j.biortech.2025.132349","url":null,"abstract":"<div><div>This study investigated the role of indigenous and cultivated microalgae in nutrient removal and biomass production in dairy wastewater, especially in microbial function change. Five indigenous and three cultured microalgal strains were grown in sterile and non-sterile dairy wastewater, and nutrient removal and biomass profiles were analysed. Results showed higher phosphorus removal (90.1 % vs. 81.8 %, <em>p</em> < 0.001) and biomass production (2.3 vs. 2.0 g/L, <em>p</em> < 0.001) in sterile wastewater, while nitrogen removal was higher in non-sterile wastewater (83.1 % vs. 77.5 %, <em>p</em> < 0.05). Indigenous strains grew more consistently in high-concentration wastewater, though not significantly different from cultured strains. Phycosphere bacteria communities were more closely associated with total nitrogen, total phosphorus, and pigment content, while free-living bacteria primarily dependent on chlorophyll <em>a</em> and extracellular polymeric substances (EPS). The nitrogen transforming function was enhanced in phycosphere. These findings provide insights for optimizing microalgal-based wastewater treatment, advancing sustainable dairy wastewater management.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132349"},"PeriodicalIF":9.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Economic and environmental analysis: Straw biogas project operating at full load with dry yellow corn straw

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-03 DOI: 10.1016/j.biortech.2025.132335

Xiaotian Chen , Wei Fu , Kai Hu , Guofeng Yin , Song Liu , Na Zhu , Yehua Zhao , Zongjun Cui , Xufeng Yuan

{"title":"Economic and environmental analysis: Straw biogas project operating at full load with dry yellow corn straw","authors":"Xiaotian Chen , Wei Fu , Kai Hu , Guofeng Yin , Song Liu , Na Zhu , Yehua Zhao , Zongjun Cui , Xufeng Yuan","doi":"10.1016/j.biortech.2025.132335","DOIUrl":"10.1016/j.biortech.2025.132335","url":null,"abstract":"<div><div>Due to the unique straw raw materials in China, the current straw biogas project based on the “two-stage” process of wet anaerobic digestion still has problems such as deficient technology, low production capacity, and weak profitability. In this study, we improved the original process for the biogas project, aiming at increasing biogas yield and profit. The results show that the new process (NP) can effectively recover the “carbon” by anaerobic hydrolysis, solve the problems of scum and crust, and significantly improve the biomethane yield (141.3%-321.8%), net profit ($599,667-$772,004/year), and carbon emission reduction equivalent (2.7×10<sup>7</sup>-4.6×10<sup>7</sup> kg CO<sub>2</sub>e/year). Based on the amount of dry yellow corn straw that can be collected annually for energy-oriented production, NP’s potential economic and environmental value is estimated to be significant. This study provides reliable technical support for efficient utilization of agricultural resources and circular economy.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132335"},"PeriodicalIF":9.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-state fermentation of distillers grains with Aspergillus pachycristatus for echinocandin B biosynthesis

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-03 DOI: 10.1016/j.biortech.2025.132334

Jiaxin Tang , Weibing Zhang , Jing Xu , Luyan Sun , Yaqi Dang , Haoyang Li , Liyan Yu , Jing Su , Ting Xue , Rui Guo , Jun Xie , Lei Wang

{"title":"Solid-state fermentation of distillers grains with Aspergillus pachycristatus for echinocandin B biosynthesis","authors":"Jiaxin Tang , Weibing Zhang , Jing Xu , Luyan Sun , Yaqi Dang , Haoyang Li , Liyan Yu , Jing Su , Ting Xue , Rui Guo , Jun Xie , Lei Wang","doi":"10.1016/j.biortech.2025.132334","DOIUrl":"10.1016/j.biortech.2025.132334","url":null,"abstract":"<div><div>An innovative strategy has been developed to utilize distillers grains (DGS) as a substrate for solid-state fermentation (SSF) to synthesize echinocandin B (ECB). In this study, the optimal fermentation parameters for <em>Aspergillus pachycristatus</em> were determined as follows: an inoculation density of 1 × 10<sup>6</sup> spores/gram dry substrate (gds), pH maintained at 6.0, temperature controlled at 30℃, moisture kept at 60% (v/w), and cultivation duration set to 6 days. Engineered <em>Trichoderma reesei</em> is employed to pretreat DGS and enhance the release of nutrient substances for subsequent utilization by <em>A. pachycristatus</em>. Furthermore, incorporating an amino acid cocktail augments metabolic flux towards ECB synthesis. Ultimately, these efforts result in a production yield of 2017.6 μg/gds for ECB. This study not only provides a solution for the high-value utilization of DGS but also presents an efficient, cost-effective, and environmentally friendly synthetic process within the circular economy framework for industrial production of ECB.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"425 ","pages":"Article 132334"},"PeriodicalIF":9.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0