Cultivation of Auxenochlorella protothecoides using tofu whey wastewater for aquaculture feed production: Integrating resource recovery and microalgal biomass valorization strategy

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-06-27 DOI:10.1016/j.biortech.2025.132913

Liang Zhong, Xing Meng, Yulong Zhang, Zongfan Peng, Rong Zhou, Yufang Tang, Yuqin Li

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Abstract

This study investigates the potential of utilizing tofu whey wastewater (TWW) for Auxenochlorella protothecoides cultivation and aquatic protein feed production. Results demonstrate that A. protothecoides cultured in TWW (AP-C-TWW) achieves biomass and protein yield of 16.45 g/L and 8.84 g/L, respectively, representing 78 % and 85 % increases compared to conventional medium. AP-C-TWW exhibits an essential-to-nonessential amino acid ratio of 0.7, fulfilling protein feed standards. Whilst TWW treatment with A. protothecoides results in the removal of 88 %, 96 %, and 99 % of total phosphorus, total nitrogen, and chemical oxygen demand, respectively, ensuring compliance with discharge regulations. Furthermore, replacing 5 % of dietary fishmeal with AP-C-TWW increases the final body weight and specific growth rate of Oncorhynchus mykiss by 10 % and 13 %, respectively, compared to control group. Additionally, it significantly enhances antioxidant capacity, innate immunity, and fillet quality. This study highlights the potential of TWW upcycling for sustainable microalgae-based aquaculture feed production.

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利用豆腐乳清废水培养水产养殖饲料用原辅小球藻：整合资源回收和微藻生物量增值策略

本研究探讨了利用豆腐乳清废水（TWW）培养原毛虫和生产水产蛋白饲料的潜力。结果表明，在TWW （AP-C-TWW）培养基中培养的原石斛生物量和蛋白质产量分别达到16.45 g/L和8.84 g/L，比常规培养基分别提高了78 %和85 %。AP-C-TWW必需与非必需氨基酸比为0.7，满足蛋白质饲料标准。在TWW处理过程中，原石菖蒲的总磷、总氮和化学需氧量的去除率分别为88 %、96 %和99 %，符合排放标准。此外，用AP-C-TWW替代饲粮中5 %的鱼粉，可使吻Oncorhynchus的最终体重和特定生长率分别比对照组提高10 %和13 %。此外，它显著提高抗氧化能力，先天免疫和鱼片质量。这项研究强调了TWW升级回收在可持续微藻水产养殖饲料生产中的潜力。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.