Bioresource Technology最新文献

{"title":"Microbial production of medium-chain-length diols: Current stage and perspectives","authors":"Chunzhe Lu ,&nbsp;Ruud A. Weusthuis","doi":"10.1016/j.biortech.2025.132932","DOIUrl":"10.1016/j.biortech.2025.132932","url":null,"abstract":"<div><div>Medium-chain-length α,ω-diols (mcl-diols) are valuable and versatile molecules with applications in polymers, lubricants, and specialty chemicals. Currently, their production relies on fossil-based industrial processes, but there is a growing effort to develop sustainable alternatives. Microbial biosynthesis has emerged as a promising approach, with successful mcl-diols production from various renewable substrates, including <em>n</em>-alkanes, fatty acids, cycloalkanes, adipic acid, and glucose. This review summarizes and compares different microbial mcl-diols biosynthetic pathways, highlighting their strengths and limitations. A generalized pathway applicable to various chain lengths is proposed, based on fatty acid biosynthesis or the reversed β-oxidation pathway. Critical challenges remain, such as accelerating microbial chassis selection and optimization, enhancing the activity of rate-limiting enzymes, and mitigating the toxicity of intermediates and end products. To accelerate the commercialization of microbial mcl-diols production, future efforts should focus on metabolic engineering strategies, advanced protein engineering techniques, and process optimization. Additionally, the integration of synthetic biology, adaptive laboratory evolution, and AI-driven enzyme design can further enhance pathway efficiency and reduce production costs. This review provides insights into the current progress and future directions for sustainable mcl-diols biosynthesis, contributing to the broader goal of replacing fossil-based chemicals with bio-based alternatives.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132932"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental impact assessment of pyrolysis and incineration including end-of-life of sewage sludge by-products","authors":"Ga-Been Lee ,&nbsp;Chae-Eun Ahn ,&nbsp;Won-Gune Jeong ,&nbsp;Yiu Fai Tsang ,&nbsp;Kitae Baek","doi":"10.1016/j.biortech.2025.132929","DOIUrl":"10.1016/j.biortech.2025.132929","url":null,"abstract":"<div><div>Incineration and pyrolysis have been proposed for sewage sludge treatment. However, most studies have overlooked the environmental impacts of by-products from thermal processes. This study compared the applicability of incineration and pyrolysis, with a detailed environmental impact assessment of by-products. The long-term stability of heavy metals in ash and biochar was investigated through an accelerated aging process, while their potential as fertilizers was evaluated in terms of phosphorus availability and other functions. Both incineration ash and pyrolyzed biochar demonstrated high heavy metal stability even after aging and exhibited potential as substitutes for chemical fertilizers. Consequently, the life cycle assessment determined that the environmental impacts of incineration were more severe than those of pyrolysis due to increased biogenic CO₂ emissions and greater mobility of heavy metals during incineration. Therefore, pyrolysis is suggested as the more environmentally sustainable option for sewage sludge treatment, considering both environmental impacts and by-product implications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132929"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-valued hexoses bioproduction from natural sugar resources: Status, trends, challenges and perspectives","authors":"Mengwei Lu ,&nbsp;Na Li ,&nbsp;Deming Rao ,&nbsp;Jing Wu ,&nbsp;Wei Xia","doi":"10.1016/j.biortech.2025.132930","DOIUrl":"10.1016/j.biortech.2025.132930","url":null,"abstract":"<div><div>The high-valued conversion of natural sugar resources is one of the priorities for global resource utilization and human health development. These conversions can not only greatly improve the efficiency of carbon resource utilization, but its diversified products are also potential candidates for alleviating the increasingly serious human health problems such as obesity and diabetes. As a novel sugar food additive, the industrial-scale production of high-valued hexoses have emerged as a critical focus in food science and biotechnology. This study evaluates established methodologies for bioproduction of high-valued hexoses, and provides a comparative assessment of technological advantages and industrial scalability limitations. Our investigation highlights the thermodynamic-driven isomerization as a promising platform for industrial applications in theory, necessitating an in-depth examination of its current technological status and developmental prospects. The thermodynamic-driven isomerization demonstrates distinct thermodynamic advantages through its innovative energy-recycling mechanism, coupled with the utilization of cost-effective starch derivatives as substrates. However, three critical challenges hinder its industrial application: (1) Insufficient catalytic properties of rate-limiting enzymes; (2) Instability of continuous bioprocessing; (3) Suboptimal atomic economy. Strategic advancements should focus on: (1) Mining novel biocatalysts through integrated approaches combining structural bioinformatics, molecular dynamics simulations, and directed evolution; (2) Developing immobilized enzyme reactors with improved stability; (3) Residual substrate recycling and by-product minimization to improve atomic economy. These synergistic improvements have the potential to substantially improve hexose conversion while significantly reducing manufacturing costs, ultimately enabling cost-competitive industrial-scale functional sweetener production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132930"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced carbon capture and medium chain fatty acid production using microbial electrosynthesis: Role of electrode surface area","authors":"Narnepati Krishna Chaitanya ,&nbsp;Akanksha Rajpurohit ,&nbsp;Pavithra S. Nair ,&nbsp;Pritha Chatterjee","doi":"10.1016/j.biortech.2025.132916","DOIUrl":"10.1016/j.biortech.2025.132916","url":null,"abstract":"<div><div>Balancing the surface area of electrodes to reactor volume (SA/V) ratio in microbial electrosynthesis (MES) systems is crucial for enhancing electron transfer, biofilm development, and product yield. Batch MES experiments were conducted using cathodes with SA/V ratios of 40 cm² L⁻¹ (MES-1), 150 cm² L⁻¹ (MES-2), 260 cm² L⁻¹ (MES-3) and 333 cm² L⁻¹ (MES-4), selected based on statistical analysis of previous studies. Among these, MES-3 (260 cm² L⁻¹) demonstrated the highest caproic acid production of 1.5 ± 0.2 g L⁻¹ and selectivity 67 %, outperforming MES-1, MES-2, and MES-4 by 2.1, 1.4, and 4.4 times, respectively. MES-3 had improved mass and electron transfer while maintaining effective microbe-electrode interactions. Additionally, MES-3 showed the lowest energy consumption (6.5 ± 2.3 kWh mol⁻¹ VFAs) and a higher electron recovery efficiency (55.8 ± 18.3 % at 2.5 V). These results demonstrate that balancing SA/V ratio is key to enhancing MES performance and sustainable MCFA production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132916"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Greenhouse gas emissions and carbon budget estimation in constructed wetlands treating aquaculture tailwater: Insight from seasonal dynamics of dissolved organic matter and microbial community","authors":"Shouzhuang Liu ,&nbsp;Lu Yao ,&nbsp;Ruonan Chen ,&nbsp;Hao Xing ,&nbsp;Jinhui Pang ,&nbsp;Liping Zhang ,&nbsp;Zhenbin Wu ,&nbsp;Qiaohong Zhou","doi":"10.1016/j.biortech.2025.132925","DOIUrl":"10.1016/j.biortech.2025.132925","url":null,"abstract":"<div><div>Constructed wetlands (CWs) effectively treat aquaculture tailwater but face challenges from greenhouse gas (GHG) emissions affecting their carbon sink potential. Few systematic studies have been conducted to identify the drivers of temporal variation of GHG emissions in CWs. Through year-round monitoring of an integrated vertical flow constructed wetland (IVCW), we identified seasonal dynamics in carbon oxide (CO₂), methane (CH₄), and nitrous oxide (N₂O) emissions, with summer fluxes exceeding winter levels by 12-fold for CH₄ and 3-fold for N₂O. Through the Mantel test, we found that the relative abundance of fulvic acid, tryptophan-like components, and functional groups related to the carbon and nitrogen cycle remarkably modified the temporal variation in CH₄ and N₂O emissions. The variation in CO₂ emission was primarily regulated by chromophoric dissolved organic matter (DOM) concentration and was indirectly influenced by water properties. Redundancy analysis revealed that water physicochemical parameters and DOM characteristics jointly explained 36.0 % and 49.7 % of the variation in bacterial composition at the phylum and genus levels, respectively, with aromaticity and molecular weight of DOM as key determinants. The IVCW functioned as a net carbon sink with a rate of annual carbon sequestration averaging 1532.36 g C m⁻² yr⁻¹, offering substantial potential for emission reductions. By elucidating how seasonal variations in DOM characteristics and microbial community structure influence GHG emissions in CWs, this study advances our understanding of the mechanisms driving the performance of CWs in terms of carbon sequestration. We propose that regular plant harvesting can enhance the role of CWs as carbon sinks.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132925"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar-microorganisms hybrid enhanced anaerobic digestion: Innovative insight considering electron transfer potential and functional network of microorganisms","authors":"Yun He ,&nbsp;Zhi Wang ,&nbsp;Caihong Shen ,&nbsp;Shilei Wang ,&nbsp;Yiyang Liu ,&nbsp;Xiaoyong Li ,&nbsp;Xueke Bai ,&nbsp;Junhao Zhao ,&nbsp;Xiaoling Zhao ,&nbsp;Xingyao Meng ,&nbsp;Yafan Cai ,&nbsp;Jingliang Xu ,&nbsp;Hanjie Ying","doi":"10.1016/j.biortech.2025.132928","DOIUrl":"10.1016/j.biortech.2025.132928","url":null,"abstract":"<div><div>Immobilizing microorganisms on biochar is potential method to regulate the electron transfer capacity. This study developed biochar-microorganism hybrids via pre-coupling for anaerobic digestion (AD) of high-load organic wastewater. Compared with uncoupled groups, the methane yield of pre-coupled groups increased by 26.4 %-36.9 %, the lag phase shortened from 4.45-5.62 days to 0.85–1.06 days, and soluble chemical oxygen demand removal efficiency increased from 60.8 %-63.8 % to 92.4 %-96.4 %. Microscopic analysis showed that pre-coupling enhanced microbial activity and abundance, reduced the bacteria-archaea spatial distance, and promoted direct interspecies electron transfer. Synergistic microbes (<em>Syntrophobacter</em> and <em>Syntrophomonas</em>) were enriched, potentially establishing syntrophic relationships with methanogens to promote organic degradation. Functional prediction indicated the potential abundance of metabolic modules including M00009, M00173, M00144, M00620, and M00374 increased. These findings provided microscopic explanations for the enhanced AD performance. The developed biochar-microorganism hybrid demonstrated the potential to reduce hydraulic retention time and improve AD efficiency, offering valuable guidance for practical applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132928"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of partial nitrification, endogenous denitrification and anaerobic ammonia oxidation in low dissolved oxygen anaerobic/oxic/anoxic-aerobic granular sludge reactor treating low carbon to nitrogen ratios wastewater","authors":"Jingwei Ma,&nbsp;Long Chen,&nbsp;Yaning Ji,&nbsp;Hui Sun,&nbsp;Ying Han,&nbsp;Liang Zhu,&nbsp;Peng Bi,&nbsp;Qiulai He","doi":"10.1016/j.biortech.2025.132923","DOIUrl":"10.1016/j.biortech.2025.132923","url":null,"abstract":"<div><div>The anaerobic/aerobic/anoxic-aerobic granular sludge (AOA-AGS) process effectively removes nitrogen while tolerating limited oxygen and carbon. However, integrating anaerobic ammonia oxidation (Anammox), which thrives under low organic carbon and oxygen conditions, with AOA-AGS remains challenging. This study investigated nitrogen removal performance and community changes in an AOA-AGS sequencing batch reactor with low carbon to nitrogen ratios (C/N) wastewater and reduced dissolved oxygen (DO) from 5-7 mg/L to 0.5 ± 0.2 mg/L. The total inorganic nitrogen removal rate stabilized at 82 ± 9 % under low DO, driven by partial nitrification and endogenous denitrification through dominant denitrifying glycogen-accumulating organisms (DGAOs), such as <em>Candidatus_Competibacter</em> (43.09 %). Anammox bacteria (mainly <em>Candidatus_Brocadia</em>) were enriched under long solids retention time (128 days) and low DO, synergizing with DGAOs for enhanced nitrogen removal. This study demonstrated that AOA-AGS under low DO enables efficient nitrogen removal through the synergistic endogenous denitrification by DGAOs and Anammox in low C/N wastewater, offering a sustainable strategy.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132923"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Saccharomyces cerevisiae for ethanol production from glycerol, xylose, acetic acid, and glucose","authors":"Sadat Mohamed Rezk Khattab ,&nbsp;Masato Katahira ,&nbsp;Takashi Watanabe","doi":"10.1016/j.biortech.2025.132921","DOIUrl":"10.1016/j.biortech.2025.132921","url":null,"abstract":"<div><div>Global bioethanol production exceeds 110 billion liters annually, yet its expansion remains constrained by the limited range of carbon sources fermentable by <em>Saccharomyces cerevisiae</em>. Glycerol—a major byproduct of biodiesel production—has recently gained attention as both a biomass pretreatment solvent and a fermentable substrate in emerging integrated biorefineries. However, native <em>S. cerevisiae</em> cannot efficiently ferment glycerol, xylose, or acetic acid, and no single engineered strain has previously demonstrated co-fermentation of all these substrates with glucose. In this study, we expanded the metabolic capacity of the previously engineered strain SK-FGG4 (capable of fermenting glycerol and glucose) to enable co-utilization of xylose and acetic acid, generating strain SK2-5. Using CRISPR-based genome editing, we replaced the native <em>ALD6</em> with a <em>Pichia stipitis</em> xylose assimilation pathway (PsXR, PsXDH), co-expressed with xylulose kinase. Mitochondrial <em>NDE1</em> and <em>NDE2</em> were replaced with <em>Salmonella enterica</em> acetylating acetaldehyde dehydrogenase (SeEutE). Overexpression of <em>JEN1</em> and a mutated <em>ACS1</em> (L707P) further enhanced acetic acid assimilation, while an additional <em>PsXDH</em> copy improved xylose fermentation efficiency. Under microaerobic conditions, strain SK2-5 achieved over 95% theoretical ethanol conversion efficiency from a mixed substrate of glycerol, xylose, acetic acid, and glucose. To our knowledge, this is the first demonstration of a single <em>S. cerevisiae</em> strain capable of efficiently co-fermenting all four carbon sources. These results establish a flexible metabolic framework for future strain development in glycerol-integrated biorefineries and support coupling with acid-catalyzed glycerolysis and biodiesel–ethanol co-production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132921"},"PeriodicalIF":9.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of humus acids in chicken manure compost by inhibiting aromatic degrading bacteria","authors":"Ruju Zhang ,&nbsp;Yingpeng Zhang ,&nbsp;Xiaomei Ye ,&nbsp;Qiuqin Ma ,&nbsp;Jin Zhou ,&nbsp;Xinya Su ,&nbsp;Yonglan Xi ,&nbsp;Jing Du ,&nbsp;Cong Wang ,&nbsp;Fei Zhu","doi":"10.1016/j.biortech.2025.132920","DOIUrl":"10.1016/j.biortech.2025.132920","url":null,"abstract":"<div><div>Microorganisms are a “double-edged sword” for humus (HS) formation in compost. Their degradation activity provides precursors for HS formation, but also leads to HS degradation in nutrient-limited environments. This study aimed to investigate the protective effect of chitosan (CTS) on HS stabilization in compost by inhibiting microbial degradation, taking advantage of CTS’s high adsorption efficiency and antibacterial properties. Compared to the control group (CK), the HS contents in the CTSQ group (0 d) and CTSH group (8 d) increased by 9.26 mg/g and 12.91 mg/g, respectively, with the CTSH group showing a significantly better effect on HS content (<em>P</em> &lt; 0.05). Furthermore, CTS significantly reduced the abundance of 18 microbial species capable of degrading aromatic compounds, including <em>Ulvibacter</em> and <em>Brevibacterium</em>, suggesting a possible mechanism by which CTS protects HS from microbial decomposition. These findings provide valuable insights for the practical application of HS stabilization technologies in composting, highlighting the potential of CTS as an effective agent for improving HS preservation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132920"},"PeriodicalIF":9.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering of Pichia pastoris as an industrial chassis enables biosynthesis of dopamine from methanol.","authors":"Dexun Fan, Zijia Yuan, Huayang Tang, Pengcheng Ren, Shuangyan Han","doi":"10.1016/j.biortech.2025.132915","DOIUrl":"10.1016/j.biortech.2025.132915","url":null,"abstract":"<p><p>Methanol, as a renewable and carbon-neutral single-carbon (C1) feedstock, has emerged as an ideal carbon source for green biomanufacturing due to its non-competition with food resources and scalability for industrial production. Here, we report the first efficient biosynthesis of dopamine from methanol through systematic metabolic engineering strategies in P. pastoris. Specifically, overexpressing high-activity tyrosine hydroxylase and enhancing shikimate pathway flux yielded 579 mg/L dopamine. To prevent degradation, key dopamine catabolic enzymes (PAS_chr1-4_0441) were knocked out. By reinforcing NADH regeneration and accelerating methanol assimilation, the titer increased to 1533 mg/L, an 84.2-fold increase from the first-generation strain. Finally, we optimized the fermentation process in a 15 L fermenter to minimize dopamine autoxidation, achieving a highest reported dopamine titer using methanol as the sole carbon source to date (12.2 g/L). This study not only validates methanol as a high-performance substrate for industrial microbiology, but also establishes a critical foundation for synthesizing dopamine and its derivatives from C1 feedstocks.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132915"},"PeriodicalIF":9.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}