{"title":"Enhancing pyrite-based bioretention systems under complex stormwater conditions using a mixed carbon source strategy: Insights into complementary carbon release, microbial restructuring, and pyrite regeneration.","authors":"Hao Zheng, Haiyuan Ma, Zheng Kong, Wenlin Zhao, Xinyue Wang, Huan Xiao, Yuanxiang Mao, Shihu Hu, Hongxiang Chai","doi":"10.1016/j.biortech.2026.135181","DOIUrl":"10.1016/j.biortech.2026.135181","url":null,"abstract":"<p><p>Pyrite-based bioretention systems hold substantial promise for the simultaneous removal of nitrogen and phosphorus from stormwater runoff. However, their performance under high-frequency and high-intensity rainfall conditions is often limited by the low electron-supply capacity of pyrite. To address this limitation, a mixed carbon source strategy was developed to enhance nutrient removal under complex rainfall conditions. Four bioretention systems, including the mixed carbon source-pyrite system (WCP), woodchip-pyrite system, corncob-pyrite system, and pyrite-only system, were constructed and systematically evaluated under simulated rainfall events with varying intensities and frequencies. Among all systems, WCP consistently achieved the best performance, with removal efficiencies of 88.1 ± 1.3% for ammonium, 86.6 ± 1.9% for nitrate, 87.0 ± 2.0% for total dissolved nitrogen, and 83.3 ± 4.3% for total dissolved phosphorus. Mechanistic analyses revealed that readily biodegradable carbon released from corncob promoted rapid heterotrophic denitrification during rainfall events, whereas recalcitrant carbon released from woodchips sustained pyrite-driven autotrophic denitrification during drying periods. Their complementary carbon-release characteristics synergistically enhanced nitrogen and phosphorus removal. In addition, mixed carbon sources reshaped the microbial community, enhanced cooperation between autotrophic and heterotrophic functional groups, and improved nitrogen transformation and electron transfer efficiency. Media characterization further showed that mixed carbon sources promoted FeS/FeS<sub>2</sub> cycling, thereby facilitating pyrite regeneration and supporting long-term operational stability. Overall, this study elucidates a multi-dimensional enhancement mechanism driven by mixed carbon sources-complementary carbon release, microbial restructuring, and pyrite regeneration-and provides a robust and sustainable strategy for nutrient control in stormwater bioretention systems under complex rainfall conditions.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135181"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148256618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Light-quality-dependent pigment remodeling and <sup>13</sup>C incorporation dynamics in Haematococcus pluvialis revealed by confocal Raman microscopy and Raman-SIP.","authors":"Hanxu Xu, Shuai Zhang, Mostafa Gouda, Jiayue Lei, Yulun Dong, Wei Luo, Yong He, Xiaoli Li, Hailiang Zhang","doi":"10.1016/j.biortech.2026.135183","DOIUrl":"10.1016/j.biortech.2026.135183","url":null,"abstract":"<p><p>Light quality critically regulates pigment remodeling and carbon allocation in Haematococcus pluvialis, yet stage-resolved, single-cell quantification of these processes remains limited. Bulk pigment assays, confocal Raman spectroscopy, Raman chemical imaging, and <sup>13</sup>C-based Raman stable isotope probing (Raman-SIP) were integrated to characterize pigment dynamics and de novo carbon incorporation across cultivation time and division stages under white, red, and blue light. A spectral correction was established to reduce carotenoid interference in the chlorophyll-related region, enabling clearer separation of pigment pools and improved agreement with bulk measurements. Blue light selectively promoted carotenoid accumulation, increased single-cell heterogeneity, and induced stage-dependent spatial reorganization, whereas red light preferentially enhanced chlorophyll-related signals. Raman-SIP revealed progressive <sup>13</sup>C incorporation into pigment-associated molecular structures under all light treatments. Blue light produced the highest labeling fraction for the carotenoid-associated 1156/1137 cm<sup>-1</sup> pair on Day 7 (52 %), whereas red light showed the highest corrected labeling fraction for the chlorophyll-related 1521/1490 cm<sup>-1</sup> pair (51 %). Raman imaging further revealed pronounced intracellular heterogeneity and hotspot-like carotenoid distributions under blue light, with transmission electron microscopy providing ultrastructural context. This integrated, stage-resolved single-cell framework directly links light-quality regulation to pigment remodeling and carbon allocation dynamics in H. pluvialis.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135183"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2026-10-01Epub Date: 2026-07-09DOI: 10.1016/j.biortech.2026.135308
Xue-Ting Wang, Yanxue Li, Yuxin Pan, Xue Xing, Bo Wang, Defeng Xing, Jun Nan, Jonathan Tian En Lee, Eakalak Khan, Daniel C W Tsang, Nan-Qi Ren, Yong Sik Ok
{"title":"Corrigendum to \"Linking microbial functional partitioning with methane productivity and stability in microbial electrolysis cell-anaerobic digestion hybrid systems\" [Bioresour. Technol. 453 (2026) 134629].","authors":"Xue-Ting Wang, Yanxue Li, Yuxin Pan, Xue Xing, Bo Wang, Defeng Xing, Jun Nan, Jonathan Tian En Lee, Eakalak Khan, Daniel C W Tsang, Nan-Qi Ren, Yong Sik Ok","doi":"10.1016/j.biortech.2026.135308","DOIUrl":"10.1016/j.biortech.2026.135308","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135308"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2026-10-01Epub Date: 2026-06-05DOI: 10.1016/j.biortech.2026.135089
Pranav Sasidharan Nair, Hyunjin Kim, Seongcheol Kang, Byoung Seung Jeon, Largus T Angenent, Byoung-In Sang
{"title":"Hydrogen supplementation improves glucose-based n-caproate production in Caproiciproducens galactitolivorans with reverse β-oxidation-associated redox remodeling.","authors":"Pranav Sasidharan Nair, Hyunjin Kim, Seongcheol Kang, Byoung Seung Jeon, Largus T Angenent, Byoung-In Sang","doi":"10.1016/j.biortech.2026.135089","DOIUrl":"10.1016/j.biortech.2026.135089","url":null,"abstract":"<p><p>Medium-chain carboxylic acids, such as n-caproate, are attractive sustainable platform chemicals. However, their microbial production is often limited by electron availability and low product selectivity. This study investigated the effect of hydrogen supplementation on glucose-based n-caproate production by Caproiciproducens galactitolivorans. Batch fermentation was performed under various hydrogen pressures (0 to 600 kPa). At 600 kPa hydrogen<sub>,</sub>n-caproate reached 8.0 g L<sup>-1</sup>, accompanied by a 66% increase in n-caproate selectivity and a tenfold increase in the intracellular redox-cofactor ratio, consistent with enhanced redox-cofactor turnover. Multi-omics analysis indicated metabolic remodeling under hydrogen-enriched conditions, including the increased abundance of key enzymes in the reverse β-oxidation pathway and redox-associated functions. In contrast, although fatty acid biosynthesis genes were transcriptionally induced, this transcriptional increase was not reflected at the protein level, suggesting a metabolic response more consistent with reverse β-oxidation-supported n-caproate synthesis than with fatty acid biosynthesis. These results support the use of hydrogen as a clean external reducing agent for improving n-caproate yield and redox efficiency in defined microbial systems. This study provides mechanistic insights into redox-driven metabolic control and selective n-caproate production in a defined microbial chain elongation system.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135089"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148194481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}