Bioresource Technology最新文献

{"title":"Engineering thermotolerant Kluyveromyces marxianus strains for resveratrol production by simultaneous saccharification and fermentation of whole slurry corn cob","authors":"Marlene Baptista ,&nbsp;Carlos E. Costa ,&nbsp;Lucília Domingues","doi":"10.1016/j.biortech.2024.131755","DOIUrl":"10.1016/j.biortech.2024.131755","url":null,"abstract":"<div><div>The microbial biosynthesis of the antioxidant compound resveratrol offers an eco-friendly and sustainable alternative to chemical synthesis or plant extraction. Here, we showed that <em>Kluyveromyces marxianus</em> strains produce <em>p</em>-coumaric acid, a key precursor of resveratrol, with higher titres achieved under increased agitation conditions. Through further strain engineering, resveratrol production was achieved using glucose, xylose, and/or ethanol as substrates. Xylose emerged as the most favourable carbon source for resveratrol production, with ethanol supplementation during xylose culture resulting in increased resveratrol titres by limiting the accumulation of the by-product xylitol. At 37 ◦C, resveratrol production from corn cob hydrolysate and whole slurry substantially increased the yield of resveratrol per sugar, reaching titres of up to 69.26 mg/L. This work shows, for the first time, resveratrol production by <em>K. marxianus</em> and from corn cob whole slurry, establishing foundations for the development of an integrated sustainable process for resveratrol production from lignocellulosic materials.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131755"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-depth recognition of mixed surfactants maintaining the enzymatic activity of cellulases through stabilization of their spatial structures","authors":"Qiangqiang Liu ,&nbsp;Meysam Madadi ,&nbsp;Salauddin Al Azad ,&nbsp;Chihe Sun ,&nbsp;Ezhen Zhang ,&nbsp;Junshu Yan ,&nbsp;Abdolreza Samimi ,&nbsp;Fubao Sun","doi":"10.1016/j.biortech.2024.131756","DOIUrl":"10.1016/j.biortech.2024.131756","url":null,"abstract":"<div><div>Mixed surfactants improve the enzymatic hydrolysis of lignocellulosic substrates by enhancing cellulase stability against heat, pH, shear, and air–liquid interface stress. Under conditions of multiple factorial stresses (50 °C, pH 4.8, 180 rpm, and 15.5 cm² air–liquid interface), cellulase with ternary surfactants (Tween 60/Triton X-114/CTAB, the molar ratio 14:5.5:1) retained 84 % of its activity after 48 h of incubation, representing 1.15 and 1.29 folds that of the cellulase activity with the single Tween 60 and with no surfactants, respectively. This is attributed to the fact that ternary surfactants possess better rheology modulation and air–liquid interface competitiveness. In addition, the computational approach demonstrated that the ternary surfactants were capable of forming stronger hydrophobic and hydrogen-bond interactions with cellulase enzymes, thus maintaining its secondary structure and preventing the detrimental α-helix to β-sheet transformation known to compromise cellulase activity. This synergy offers valuable insights into surfactant-cellulase interactions and supports efficient enzymatic hydrolysis in biorefineries.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131756"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of a novel organic-inorganic composite sludge bioflocculant (SBF) from dewatered sludge as raw material: Characteristics, flocculation mechanism and application for domestic sewage","authors":"Jingyun Ge ,&nbsp;Hongyu Tian ,&nbsp;Lin Li ,&nbsp;Yunping Han ,&nbsp;Jianwei Liu","doi":"10.1016/j.biortech.2024.131747","DOIUrl":"10.1016/j.biortech.2024.131747","url":null,"abstract":"<div><div>In this work, a green sludge bioflocculant (SBF) was prepared via chemical hydrolysis of dewatered sludge and applied to flocculation of domestic wastewater. The process parameters for the preparation of the SBF were 1.80 % hydrochloric acid concentration, 60 min extraction time, and 4000 r/min centrifugation speed. SBF is polymeric flocculant composed of organic and inorganic compounds. Flocculation efficiency reached 97.31 ± 0.26 % under optimal flocculation conditions. Charge neutralization promotes the surface adsorption, bridging and net trapping and sweeping of Fe (OH)₃, Al (OH)₃ and active functional groups O–H/N–H and C = O in SBF, which together achieve efficient flocculation reactions. SBF had high efficiency and stable flocculation performance for phosphorus in urban domestic wastewater, and the concentration of TP in effluent was lower than 0.30 mg/L. Therefore, SBF prepared from dewatered sludge has efficient flocculation properties and is suitable for removing pollutant phosphorus, which has good application prospects in the field of wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131747"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"re-aerobic treatment and dissolved oxygen regulation in full-scale aerobic-hydrolysis and denitrification-aerobic process for achieving simultaneous detoxification and nitrification of coking wastewater","authors":"Heng Zhang ,&nbsp;Chaohai Wei ,&nbsp;Acong Chen ,&nbsp;Xiong Ke ,&nbsp;Zemin Li ,&nbsp;Zhi Qin ,&nbsp;Yuxin Tian ,&nbsp;Haizhen Wu ,&nbsp;Guanglei Qiu ,&nbsp;Shuang Zhu","doi":"10.1016/j.biortech.2024.131754","DOIUrl":"10.1016/j.biortech.2024.131754","url":null,"abstract":"<div><div>The biological treatment of coking wastewater is a challenge. The application of prepositioned aerobic process has rarely been systematically reported, among which the detoxification and nitrification performance of the prepositioned aerobic unit (O1) is worthy of investigation. Results indicate that O1 achieves stable simultaneous detoxification and nitrification by regulating the dissolved oxygen, effectively maintaining ammonification, nitrosation, and complete nitrification phases. Microbial community structure, metabolic pathways and functional genes showed different preferences at different phases. High dissolved oxygen concentrations (2.20–3.00 mg/L) benefited the enrichment of carbon and nitrogen related major metabolic pathways and functional genes. BOD₅/COD_Cr ratio, dissolved oxygen and toxic pollutants together shaped microbial community structure and nitrogen transformation processes. Based on the principle of DO regulation, it could assemble a biotransformation compartment for nitrogen removal from complex wastewaters through a pollutant detoxification mechanism of rapid microbial proliferation，and provides a promising approach for toxic industrial wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131754"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial synergy and relay for thermophilic hydrogen production through dark fermentation using food waste","authors":"Geunhee Kim ,&nbsp;Kyung-Suk Cho","doi":"10.1016/j.biortech.2024.131748","DOIUrl":"10.1016/j.biortech.2024.131748","url":null,"abstract":"<div><h3>Background</h3><div>Food waste is a significant global issue, with 1.3 billion tons generated annually, a figure expected to rise to 2.1 billion tons by 2030. Conventional disposal methods, such as landfilling and incineration, present environmental challenges, including methane emissions and pollution. Hydrogen production through dark fermentation presents a sustainable alternative, offering both waste management and renewable energy generation. This study investigates the bacterial synergy and relay mechanisms involved in thermophilic H₂ production using food waste as a substrate.</div></div><div><h3>Purpose</h3><div>The primary aim of this research was to analyze the metabolic pathways and dynamics of functional genes prediction during thermophilic H₂ production from food waste, focusing on the role of bacterial consortia in enhancing H₂ yields.</div></div><div><h3>Methods</h3><div>A continuous stirred-tank reactor (CSTR) was operated using food waste as the substrate and a thermophilic bacterial consortium as the inoculum. The study utilized genomic analysis to monitor changes in bacteriobiome composition over time and to correlate these changes with H₂ production. Volatile fatty acids (VFAs) and H₂ production rates were analyzed using gas chromatography and high-performance liquid chromatography (HPLC). The Kyoto Encyclopedia of Genes and Genomes (KEGG) database was employed to identify functional genes involved in the fermentation process.</div></div><div><h3>Results</h3><div>The study identified key bacterial species, including <em>Caproiciproducens</em> and <em>Caproicibacter</em>, that dominated during the later stages of H₂ production, replacing earlier dominant species such as <em>Clostridium</em>. These shifts in bacterial dominance were strongly correlated with sustained H₂ production rates ranging from 353 to 403 mL·L⁻¹·h⁻¹, with H₂ concentrations between 55 % and 62 % (v/v). Functional gene analysis revealed significant pathways related to polysaccharide degradation, glycolysis, and dark fermentation.</div></div><div><h3>Conclusions</h3><div>This study highlights the importance of bacterial synergy and relay in maintaining continuous H₂ production from food waste under thermophilic conditions. The findings provide insights into optimizing biohydrogen production processes, emphasizing the role of specific bacterial species in enhancing efficiency. These results contribute to the development of sustainable waste management strategies and renewable energy production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131748"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between SDBS and Hydrilla verticillata − epiphytic biofilm in wetland receiving STPs effluents: Nutrients removal and epiphytic microbial assembly","authors":"Xin Lv ,&nbsp;Songhe Zhang ,&nbsp;Shaozhuang Guo ,&nbsp;Xiuren Hu ,&nbsp;Hezhou Chen ,&nbsp;Zheng Qiu ,&nbsp;Yuexiang Gao ,&nbsp;Aiyu Qu","doi":"10.1016/j.biortech.2024.131750","DOIUrl":"10.1016/j.biortech.2024.131750","url":null,"abstract":"<div><div>The fate and effects of sodium dodecyl benzene sulfonate (SDBS) in sewage treatment plants effluents on nutrients and submerged macrophytes are far from clear in wetlands. This study conducted a 24-day experiment to investigate changes in nutrients and epiphytic biofilm of <em>Hydrilla verticillata</em> in wetlands receiving effluents with 0.5, 2 and 5 mg L⁻¹ SDBS. The decrease of SDBS in overlying water followed pseudo-first-order kinetic equation, with over 80 % of SDBS removal achieved. 2 and 5 mg L⁻¹ SDBS decreased nutrient removal efficiency, induced oxidative stress response and damaged cells of <em>H. verticillata</em>. SDBS altered bacterial and eukaryotic community diversity. 0.5 mg L⁻¹ SDBS can promote carbon fixation and methane oxidation of microorganisms. Network analysis revealed that 0.5 mg L⁻¹ SDBS decreased the stability of epiphytic ecosystems. Mantel tests indicated significant influences of SDBS, temperature, and total nitrogen on epiphytic microbial communities.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131750"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vacuum evaporation coupled with anaerobic digestion for process intensification and ammonia recovery: Model development, validation and scenario analysis","authors":"Amr Mustafa Abdelrahman ,&nbsp;Ali Khadir ,&nbsp;Domenico Santoro ,&nbsp;Eunkyung Jang ,&nbsp;Ahmed Al-Omari ,&nbsp;Chris Muller ,&nbsp;Katherine Y. Bell ,&nbsp;John Walton ,&nbsp;Damien Batstone ,&nbsp;George Nakhla","doi":"10.1016/j.biortech.2024.131753","DOIUrl":"10.1016/j.biortech.2024.131753","url":null,"abstract":"<div><div>A mathematical model for vacuum evaporation process was developed, which was experimentally validated at different initial pHs and temperatures for ammonia removal from anaerobically digested sludge. Six scenarios were evaluated by combining vacuum evaporation process with anaerobic digestion using anaerobic digestion model 1. These scenarios included a control, a pretreatment by vacuum evaporation, a post-treatment by vacuum evaporation at pH 9, a post-treatment by conventional evaporation (100 °C), an intensification with vacuum-concentrated recycled digestate back to the digester, and a second intensification at pH 9. Results indicated that using the evaporator as post-treatment at pH 9 or for intensification at pH 9 were the most favorable options, recovering more than 76 % of the nitrogen present in influent sludge with no negative effect on methane production. An economic analysis showed that the intensification at pH 9 was cost-neutral, significantly higher than the net present value of the control scenario (–22 M$).</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131753"},"PeriodicalIF":9.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of quaternary ammonium disinfectants on human pathogenic bacteria in anaerobic sludge digestion: Dose-response and resistance variation","authors":"Wan Yang ,&nbsp;Yunfei Liang ,&nbsp;Shengsen Wang ,&nbsp;Chen Cai ,&nbsp;Xiaozhi Wang ,&nbsp;Xiaohu Dai ,&nbsp;Xiang Chen","doi":"10.1016/j.biortech.2024.131745","DOIUrl":"10.1016/j.biortech.2024.131745","url":null,"abstract":"<div><div>Sewage sludge is a critical reservoir for biological pollutants, and its harmless disposal remains a major issue. Quaternary ammonium compounds (QACs) as typical household disinfectants are inevitably concentrated in sewage sludge, and have the potential to affect human pathogenic bacteria (HPBs) that remain poorly understood. This study found that the relative abundance of HPBs in digesters was decreased by 10 − 20 % at low QACs dose, but increased by 238 − 591 % at high QACs dose. Mechanistic analysis revealed that low QACs doses promoted functional hydrolytic/fermentative bacteria and their metabolism by stimulating extracellular polymeric substances secretion and enhancing resistance to QACs. Conversely, high QAC doses decreased microbial biomass and developed QACs and antibiotic resistance of HPBs by increasing cell membrane permeability and triggering oxidative stress, resulting in deteriorating sanitation performance. These findings provide advanced insights into the potential function and hazards of exogenous QACs on the biosafety of digestate.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131745"},"PeriodicalIF":9.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of volatile fatty acids to extremely high content in fermentation of food waste: Optimization of conditions, microbial functional genes, and mechanisms","authors":"Yujie Sun,&nbsp;Yujiao Sun,&nbsp;Xueqian Ren,&nbsp;Yuanyan Xuan,&nbsp;Meijun Liu,&nbsp;Guomin Bai,&nbsp;Fan Jiang","doi":"10.1016/j.biortech.2024.131735","DOIUrl":"10.1016/j.biortech.2024.131735","url":null,"abstract":"<div><div>The engineering application of volatile fatty acids (VFA) production from food waste (FW) can significantly enhance resource utilization. Enhancing VFA production is crucial for advancing this engineering application. This study presented a economically-feasible method to achieve high VFA production from FW: Conducting fermentation at pH 9 and 37 ℃ with addition of 20 % anaerobic sludge significantly increased the conversion of FW to VFAs (80.56 g COD/L, accounting for 87.37 % of the soluble chemical oxygen demand), while also increasing the content of NH₄⁺-N (2658.15 mg/L). Macrotranscriptomic sequencing showed that <em>Anaerosalibacter</em>, <em>Amphibacillus</em>, <em>Wansuia</em>, <em>Clostridiisalibacter</em>, unclassified <em>Tissierellia</em>, <em>Massilibacterium</em>, unclassified <em>Bacteroidales</em>, and <em>Tissierellia</em> were the key active microorganisms for VFA production. The expression abundance of functional enzymes and genes related to VFA production pathways increased during the fermentation. This study significantly advanced the practical application of VFA production from FW, offering both theoretical insights and bacterial resources.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131735"},"PeriodicalIF":9.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of dissolved CO2 to control methane and acetate production in methanation reactor","authors":"F. Paillet ,&nbsp;E. Crestey ,&nbsp;G. Gaval ,&nbsp;M. Haddad ,&nbsp;F. Lebars ,&nbsp;O. Nicolitch ,&nbsp;P. Camacho","doi":"10.1016/j.biortech.2024.131722","DOIUrl":"10.1016/j.biortech.2024.131722","url":null,"abstract":"<div><div>This study investigated the influence of dissolved CO₂ on the selection of metabolic pathway using a methanation membrane bioreactor supplied with H₂/CO₂. Various ratios of H₂/CO₂ were applied (3.3, 3.8, 4.0, 4.5, and 5.0 (v/v)) to manipulate dissolved CO₂ levels in the medium. The findings revealed a correlation between the concentration of dissolved CO₂ and the production of CH₄ (positive) and acetate (negative). Specifically, at a dissolved concentration of CO₂ above 2.0 ± 0.2 mmol/L, production of CH₄ was favored. At the opposite, acetate production was favored at lower dissolved CO₂ concentrations, with a maximum concentration of 1.9 g/L observed at 0.9 mmol/L of dissolved CO₂. This study demonstrates that the modification of dissolved CO₂ levels in a methanation bioreactor can provide a strategy for the selection of metabolic pathways and microbial communities, thereby offering a promising opportunity for optimizing the conversion of CO₂ into high-value products such as CH₄ and acetate.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131722"},"PeriodicalIF":9.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}