Bioresource Technology最新文献

{"title":"Enhanced fermentation for lactic acid production from food waste via hydrothermal pretreatment: performance evaluation and metagenomic analysis.","authors":"Zuohong Chen, Jiayu Zhang, Lin Lin, Yingfei Sun, Xiao-Yan Li, Bing Li","doi":"10.1016/j.biortech.2025.133456","DOIUrl":"10.1016/j.biortech.2025.133456","url":null,"abstract":"<p><p>Mixed-culture lactic acid fermentation of food waste (FW) commonly requests low pH conditions. However, this can pose the challenge of insufficient substrate hydrolysis. To address this, we investigated hydrothermal pretreatment coupled with centrifugal supernatant recovery as a technical strategy to enhance the FW hydrolysis and lactic acid production. The study characterized the supernatant substrate after the pretreatment at different temperatures (80-140 °C), focusing on organic solubilization, supernatant-solid separation for organic recovery, grease removal, and subsequently evaluated lactic acid production in 8-day batch fermentation. The results showed that hydrothermal pretreatment significantly boosted FW hydrolysis, improved organic recovery in the supernatant, and enhanced lactic acid yields. Optimal lactic acid production (12.4 g/L) representing a 68 % increase over the control without hydrothermal pretreatment, was achieved at 120 °C, despite the maximum hydrolysis occurring at 140 °C. Metagenomic sequencing further revealed that the pretreated substrate fostered the development of a lactic acid bacteria-dominated microbial community at low pH, notably enriched with Lactobacillus amylolyticus and Lactobacillus delbrueckii, along with functional genes associated with lactic acid production. Crucially, the absence of genes related to propionate and butyrate pathways in the dominant bacteria would explain the reduced byproduct spectrum and enhanced fermentation stability. These findings indicate that hydrothermal pretreatment improves both the quantity and quality of FW supernatant substrate, promoting a lactic acid bacteria-dominated community that drives favorable metabolic pathways under low pH conditions for more efficient and stable lactic acid production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133456"},"PeriodicalIF":9.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient fractionation of lignocellulosic biomass with in-situ generated diformylxylose as renewable solvent","authors":"Xianqing Lv,&nbsp;Zhuonan Zhang,&nbsp;Zhenggang Gong,&nbsp;Guangxu Yang,&nbsp;Li Shuai","doi":"10.1016/j.biortech.2025.133452","DOIUrl":"10.1016/j.biortech.2025.133452","url":null,"abstract":"<div><div>Development of sustainable solvents for biomass biorefining remains a pivotal challenge of green chemistry. This study presented a circular biorefining strategy that valorized hemicelluloses into biobased solvent diformylxylose (DFX; Hansen solubility parameter: 21.4 MPa^−1/2) for acetal-protection pretreatment. The DFX-H₂O cosolvent system (90/10, w/w), with addition of formaldehyde and HCl, showed a decent yield of acetal-protected lignin (84.7%) and enzymatic conversion of acetal-protected cellulose after deprotection by acid hydrolysis (82.9%). Crucially, hemicelluloses-derived xylose was concurrently converted into additional DFX in situ during pretreatment, sustaining the delignification process. The spent DFX (including newly formed) was effectively recovered by crystallization and the isolated acetal-protected lignin was upgraded to monomers via hydrogenolysis at near-theoretical yields (46.5%). This work demonstrates a self-reinforcing cycle where a biomass component is transformed into the key solvent enabling efficient fractionation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133452"},"PeriodicalIF":9.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric field-driven methanogenesis from low-rank coal: Deciphering synergistic role of direct interspecies electron transfer and conventional microbial pathways.","authors":"Qing Feng, Chengwei Sun, Yong Zhao, Shaojie Yang, Hao Zi, Yingkun Zhang, Hongda Pan, Xinjian Zhang","doi":"10.1016/j.biortech.2025.133457","DOIUrl":"10.1016/j.biortech.2025.133457","url":null,"abstract":"<p><p>Low-rank coal represents a challenging substrate for biomethane production due to its low biodegradability, limiting conventional bioconversion efficiencies. This study employed an Electric Field-driven Anaerobic Digestion (EFAD) system to enhance methane production from lignite by stimulating both direct interspecies electron transfer (DIET) and classical methanogenic pathways (hydrogenotrophic and acetoclastic). Batch experiments demonstrated a 3.8-fold increase in methane yield under EFAD compared to conventional anaerobic digestion, with DIET accounting for approximately 22 % of methane production. Electrochemical impedance spectroscopy revealed reduced charge transfer resistance and enhanced redox activity, indicating improved electron transfer in the EFAD system. Microbial community analysis showed enrichment of electroactive bacteria and methanogens. Selective inhibition of methanogenic pathways confirmed the participation of targeted methanogens and highlighted the EFAD system's ability to mitigate pathway suppression via DIET enhancement. These results provide mechanistic insights that advance the potential of bioelectrochemical methods for efficient, scalable biomethane recovery from low-rank coal.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133457"},"PeriodicalIF":9.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of biowaste into functional additives for membrane-based investigation of separation and fouling in polysaccharides and polyphenols production.","authors":"Hemanth Kumar K, A S Anjana Krishnan, T Swathi, R Logith, D S Imaya, G Arthanareeswaran, Mangalaraja Ramalinga Viswanathan","doi":"10.1016/j.biortech.2025.133421","DOIUrl":"10.1016/j.biortech.2025.133421","url":null,"abstract":"<p><p>Membrane fouling is a persistent limitation in membrane-based water purification. In this study, polyethersulfone was modified with biowaste-derived functional additives such as cellulose from waste newspaper, nitrogen-doped activated carbon from fish scales, and silica from sugarcane bagasse to enhance antifouling performance during the production of polysaccharides and polyphenols. The modified membranes exhibited improved hydrophilicity, from 77.6° (pristine PES) to 58.5° (cellulose-based). The incorporation of cellulose gives the highest flux recovery ratio (78 %), driven by its superior hydrophilicity and resistance to irreversible fouling. Hermia's model analysis (R2 > 0.95) confirmed that fouling was predominantly governed by complete and intermediate blocking, while standard blocking appeared selectively depending on foulant type and material composition. Overall, cellulose demonstrated good antifouling capability, while silica provided a balanced morphology with reversible fouling under polysaccharide filtration. These results highlight the promise of biowaste valorisation for developing sustainable and membrane materials for polysaccharides and polyphenols production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133421"},"PeriodicalIF":9.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modular assembly of dual-template artificial antibody-antigen-directed co-immobilization of bioreactor for in-situ synthesis of epoxy compounds: Leveraging oxidized nicotinamide adenine dinucleotide cofactor to enhance yield","authors":"Meishan Guo ,&nbsp;Hongli Chao ,&nbsp;Yang Sun ,&nbsp;Dongyu Gu ,&nbsp;Jing Wang ,&nbsp;Yi Wang ,&nbsp;Dajun He ,&nbsp;Dezhi Huang ,&nbsp;Jing Tian ,&nbsp;Yi Yang","doi":"10.1016/j.biortech.2025.133453","DOIUrl":"10.1016/j.biortech.2025.133453","url":null,"abstract":"<div><div>Enzymatic epoxidation offers eco-friendly advantages over chemical methods. This work developed a modular dual-enzyme reactor through artificial antibody-antigen directed immobilization for in-situ epoxide biosynthesis. Magnetic artificial antibodies were synthesized using hydroquinone and 4-aminopyrimidine templates, while 2,5-dihydroxybenzaldehyde and 4-aminopyrimidine-5-carbaldehyde were employed to modify glucose oxidase (GOx) and lipase (LIP), respectively, forming artificial antigens. The dual-enzyme reactor was constructed through affinity recognition between artificial antibodies and antigens, a modular strategy that allowed rational regulation of enzyme ratios in the co-immobilized system to match cascade reaction requirements, thereby promoting optimal yield. This GOx-LIP dual-enzyme reactor achieved alkene epoxidation with epoxide yields exceeding 70% under optimized conditions. Notably, the yield could be further enhanced to 90% by incorporating oxidized nicotinamide adenine dinucleotide as a cofactor. This modular biological platform demonstrates the superiority of multi-enzyme cascade catalysis in alkene epoxidation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133453"},"PeriodicalIF":9.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directional enrichment of functional microbial communities and mutual regulation of nitrogen and phosphorus metabolism pathways during rural wastewater treatment using food waste fermentation residue","authors":"Xinjuan Li ,&nbsp;Jiaqi Meng ,&nbsp;Li Wang ,&nbsp;Zhen Zhou ,&nbsp;Zhichao Wu ,&nbsp;Qiaoying Wang","doi":"10.1016/j.biortech.2025.133448","DOIUrl":"10.1016/j.biortech.2025.133448","url":null,"abstract":"<div><div>Using fermentation residue of food waste (FRFW) as an external carbon source could efficiently promote the rural sewage treatment. In this study, the improvement mechanisms and characteristics of non-sterilized (NS)-FRFW and sterilized (S)-FRFW as supplementary carbon sources in the long-term operation of anaerobic-anoxic-aerobic membrane bioreactor were investigated. The results showed that NS-FRFW and S-FRFW increased TN removal to 7.1 and 6.1 times that of the control, and TP removal to 2.1 and 1.4 times, respectively. FRFW induced microbial community restructuring, resulting in significant enrichment of key functional taxa such as Gammaproteobacteria and Chitinophagaceae. Through quorum sensing, it specifically activated electron transfer (<em>coxA/coxB</em>) and carbon metabolism, thereby increasing the supply of nicotinamide adenine dinucleotide (NADH). This significantly upregulated the expression of amoA and nosZ (4.6 times and 1.4 times for NS-FRFW), promoting ammonia oxidation and denitrification. This study provided a theoretical basis for waste resources recycling and low-carbon wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133448"},"PeriodicalIF":9.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of banana peels to highly effective catalysts for micropollutant degradation: Effects of hydrothermal carbonization conditions.","authors":"Fernando Oscar Hayrera, Do-Gun Kim, Seok-Oh Ko","doi":"10.1016/j.biortech.2025.133442","DOIUrl":"10.1016/j.biortech.2025.133442","url":null,"abstract":"<p><p>In this study, hydrochars were prepared under various hydrothermal carbonization (HTC) conditions and pyrolyzed to fabricate hydropyrochars under the hypothesis that hydrochars are good precursors of pyrochars and that their reactivity and characteristics are controlled by the HTC conditions. For hydrochars and hydropyrochars, graphitization degree, defects, and graphitic/pyridinic/pyrrolic N increased, whereas the stacking degree and number of acidic O-containing groups decreased as the HTC temperature and time increased. The characteristics of the hydrochars significantly affected those of the hydropyrochars, whereas those of the pyrochar were clearly different. In the presence of peroxydisulfate (PDS), acetaminophen (ACT) removal was negligible for hydrochars + PDS, poor for pyrochar + PDS, and excellent for hydropyrochars + PDS. The best ACT removal was >99 % in 20 min with a 20 mg/L hydropyrochar from a hydrochar prepared via HTC at 250 ℃ for 3 h (HY250/03-PY) (PDS 0.05 mM, ACT 5 mg/L). For the hydropyrochars, possible reactive sites, such as the graphitic structure, CO, and pyridinic/graphitic-N, correlated well with the ACT removal rate. Despite the large differences in the performance and characteristics, the dominant reactive species (¹O₂) and ACT degradation pathways were similar in HY250/03-PY + PDS and pyrochar + PDS. However, HY250/03-PY was significantly superior to pyrochar in ¹O₂ generation and PDS adsorption. HY250/03-PY + PDS showed significant inhibition by coexisting ions and natural organic matter; however, most of these effects were overcome by simply increasing HY250/03-PY. These results strongly suggest that hydropyrochars with excellent reactivity and field application potential can be prepared by combining HTC control and pyrolysis.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133442"},"PeriodicalIF":9.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep eutectic solvent-based protein recovery from microalgal biomass grown in piggery wastewater","authors":"David Moldes ,&nbsp;Patricia F. Requejo ,&nbsp;Marisol Vega ,&nbsp;Silvia Bolado","doi":"10.1016/j.biortech.2025.133446","DOIUrl":"10.1016/j.biortech.2025.133446","url":null,"abstract":"<div><div>Microalgae are a promising alternative to address wastewater treatment while converting wastewater nutrients into valuable protein-rich biomass, providing a dual-purpose solution. This study investigates protein recovery from microalgal biomass based on <em>Scenedesmus almeriensis</em> cultivated in swine wastewater using deep eutectic solvents (DESs) and their water mixtures. The behavior of two DESs (choline chloride:urea and choline chloride:glycerol at a 1:2 molar ratio) was evaluated with varying amounts of water. Both DESs outperformed water in protein extraction, and adding water to the DES:biomass mixture improved recoveries. The impact of the experimental factors was evaluated using a factorial design. The highest protein yield (16.8 %) was obtained with DES ChCl:2Urea with 81.6 % of water, by milling discs, a 12:1 DES:biomass ratio and extraction at 30 °C by 0.5 h. Despite moderate yields, this work underscores the potential of aqueous solutions of DESs as green solvents for protein recovery from microalgae.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133446"},"PeriodicalIF":9.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of waste activated sludge hydrolysate digestion efficiency via promotion of direct interspecies electron transfer","authors":"Om Prakash ,&nbsp;Alsayed Mostafa ,&nbsp;Masoud Makian ,&nbsp;Mohamad Ali Mohit ,&nbsp;Ali Ashraf Joolaei ,&nbsp;Chungnam Moon ,&nbsp;Dong-Hoon Kim","doi":"10.1016/j.biortech.2025.133449","DOIUrl":"10.1016/j.biortech.2025.133449","url":null,"abstract":"<div><div>Anaerobic digestion (AD) of waste activated sludge (WAS) yields low methane (CH₄) because extracellular polymeric substances hinder hydrolysis and limit its biodegradability. Pretreatment methods such as alkaline or mechanical disruption can enhance solubilization; however, the resulting hydrolysate often contains recalcitrant compounds that inhibit further degradation. In this study, a combination of alkali along with ultrasonication was applied to enhance the solubilization of WAS, followed by CH₄ production under AD. Pretreated results showed alkaline (pH = 12) + ultrasonication (30 min) showed 300 % higher solubilization compared to ultrasonication (60 min) alone. Batch experiments (with and without Fe₃O₄) were conducted, and the results showed that pretreated hydrolysate supplemented Fe₃O₄ showed higher CH₄ yield than their control counterparts (up to 85 %). To validate the batch results of pretreated hydrolysate, a continuous operation was conducted without (Control) and with an electric voltage reactor (EVR) at different organic loading rates (OLR) up to 4 g chemical oxygen demand (COD)/L/d. The results showed that EVR enhanced the CH₄ production by 28 % and COD removal by 19 % at 4 g COD/L/d compared to the control. Microbial community analysis highlighted the dominance of <em>Syntrophomonas zehnderi</em> (a fatty acid oxidizer) in EVR, which increased by 27 %, suggesting stronger syntrophic partnerships with methanogens. Genetic profiling further supported these findings, showing a 25 % upregulation in Adenosine triphosphatease related genes and a striking 69 % increase in pili-associated genes, both critical for direct interspecies electron transfer. These results demonstrated that the combined pretreatment (alkali + ultrasonication) offers a promising alternative for enhanced AD of WAS.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133449"},"PeriodicalIF":9.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of integrated system combining parallel-connected microbial fuel cells and microbial electrolysis cell for green hydrogen production.","authors":"Moungsung Kim, Junsang Park, Woowon Jeong, Hyunjin Kim, Eunchan Jung, Bongkyu Kim","doi":"10.1016/j.biortech.2025.133445","DOIUrl":"10.1016/j.biortech.2025.133445","url":null,"abstract":"<p><p>The growing demand for sustainable wastewater valorization and renewable hydrogen energy has amplified the need for systems capable of recovering energy and producing hydrogen without external electricity input. In this study, ten single microbial fuel cells (MFC) were connected in parallel to enhance power generation, and the electricity produced was controlled using a power management system. The energy was then directly supplied to a microbial electrolysis cell (MEC) in real time and stored in a battery prior to the MEC operation. When directly connected, the MFC-MEC system achieved a hydrogen production rate of 0.08 m³/m³/d, while the battery-assisted mode reached 0.66 m³/m³/d, demonstrating an eight-fold increase. These findings demonstrate the feasibility of integrated MFC-MEC systems for self-powered hydrogen production, offering a potential pathway for transforming organic wastewater into hydrogen as a renewable energy carrier.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133445"},"PeriodicalIF":9.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}