Bioresource Technology最新文献

{"title":"Mechanistic insights into superior biofilm formation with heterotrophic nitrification-aerobic denitrification bacteria under polypropylene microplastic stress.","authors":"Yan Lin, Bixiang Nie, Xilong Liu, Qian Zhang","doi":"10.1016/j.biortech.2026.135159","DOIUrl":"10.1016/j.biortech.2026.135159","url":null,"abstract":"<p><p>Microplastics may disturb microbial activity and biofilm development in biological wastewater treatment systems, yet the response of three-dimensional rotating biological contactor start-up biofilms to polypropylene microplastic stress remains unclear. This study evaluated a biofilm initiation strategy using heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria (H-3D-RBCs) and compared it with activated sludge-inoculated systems (A-3D-RBCs) under polypropylene microplastic (PP-MP) exposure. H-3D-RBCs showed superior resistance to PP-MP disturbance, with total nitrogen removal decreasing by only 14 %, compared with an approximately 60 % decline in A-3D-RBCs. Respiratory activity inhibition remained below 15 % in H-3D-RBCs but exceeded 90 % in A-3D-RBCs. 16S rRNA gene sequencing showed that PP-MP reduced species richness and diversity in A-3D-RBCs and was associated with a > 90 % loss of core denitrifying genera, including Corynebacterium and Pseudoxanthomonas, whereas H-3D-RBCs maintained community stability and enriched Pseudoxanthomonas to 13.8 %. Metagenomic analysis indicated that PP-MP impaired nitrification and denitrification potential in A-3D-RBCs, as reflected by decreased genes encoding AMO and HAO, a 51.78 % decrease in nosZ abundance, and enhanced dissimilatory nitrate reduction to ammonium (DNRA), which likely intensified competition with denitrification and promoted nitrogen conversion to ammonia. In contrast, H-3D-RBCs suppressed DNRA and maintained high nosZ abundance. Untargeted metabolomics further showed that PP-MP was associated with metabolic disorders in A-3D-RBCs, especially disruptions in alanine, aspartate, and glutamate metabolism and arginine biosynthesis, whereas H-3D-RBCs preserved these key nitrogen metabolic processes. Overall, this study identifies key vulnerabilities of nitrogen-removal biofilms under PP-MP disturbance and provides multi-omics evidence to support the development of microplastic-resistant biofilm wastewater treatment systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135159"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced lignin-to-lipid bioconversion via glucose-assisted co-metabolism in newly isolated bacteria.","authors":"Yongbing Li, Siyi Yang, Jiali Wang, Sihu Zhang, Canfang Tang, Wen Cao, Liejin Guo","doi":"10.1016/j.biortech.2026.135160","DOIUrl":"10.1016/j.biortech.2026.135160","url":null,"abstract":"<p><p>Lignin-to-lipid bioconversion is a promising approach for producing biodiesel precursors from renewable biomass, but is limited by low conversion efficiency and the scarcity of efficient microbial strains. Here, three newly isolated bacterial strains (L77, L78, and L90) were evaluated for lipid production from dealkaline lignin and lignin-derived aromatic compounds. All strains showed better growth and lipid accumulation on lignin-derived aromatics than on polymeric lignin. Glucose-assisted co-metabolism markedly improved lignin degradation and lipid biosynthesis. The maximum lipid titers reached 373.3±17.0 mg/L for L77, 527.0±11.8 mg/L for L78, and 223.3±5.4 mg/L for L90, while the highest lipid content was obtained with L78 (65.3±2.7%). Fatty acid profiling showed that C16:0 and C18:0 were the dominant components, accounting for approximately 70% of total fatty acids, indicating favorable properties for biodiesel production. These results expand the diversity of bacterial resources for lignin valorization and demonstrate the potential of newly isolated strains for sustainable microbial lipid production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135160"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manure inoculation shapes arsenic speciation and phase partitioning during biodegradation of Pteris vittata biomass","authors":"Hao Zhang,Qi-Shang Zhou,Jia-Xiong Wu,Song-Ge Deng,Chen-Jing Liu,Wumei Xu,Lena Q. Ma","doi":"10.1016/j.biortech.2026.136030","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136030","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"22 1","pages":"136030"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methane removal from air by methanotrophic biofilms in a multi-tray reactor","authors":"Lian He,Godwin Gati,Mary Lidstrom,Q. Peter He,Jin Wang","doi":"10.1016/j.biortech.2026.135998","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135998","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"41 1","pages":"135998"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Sequential enzymatic pretreatment and Saccharomyces boulardii fermentation of cactus cladode biomass for bioactive food ingredients and cell culture serum alternatives” [Bioresour. Technol. 464 (2027) 135894]","authors":"Wasitha P.D.W. Thilakarathna,Setareh Shiroodi,Deepak K. Jha,Vikas Dadwal,Bhimanagouda Patil,Reza Ovissipour","doi":"10.1016/j.biortech.2026.135950","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135950","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"25 1","pages":"135950"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ sludge reduction assisted by alternating electrode-based electrochemical treatment.","authors":"Xiaolei Zhang, Quanze Liu, Shaoping Luo, Yingxue Sun, Yong Li, Wenyi Dong, Patrick Drogui, Ji Li","doi":"10.1016/j.biortech.2026.135135","DOIUrl":"10.1016/j.biortech.2026.135135","url":null,"abstract":"<p><p>The massive production of sludge from municipal wastewater treatment plants poses a significant challenge to its disposal and management. This study proposes a sustainable in situ sludge reduction technology. A sequencing batch reactor (SBR) was used to investigate the process feasibility. The activated sludge from the SBR was first subjected to pH adjustment to dissociate the extracellular polymeric substances (EPS) of the microorganisms, which serve as a protective layer. The vulnerable microorganisms are then treated by electrochemical oxidation, which disrupts the cells and releases the organic matter. Equal volumes of sludge were treated at pH 2 and 12, respectively, and combined for 20 min electrochemical treatment at 15 V with a 2 cm electrode spacing, a 3 mL dosage of 1 M CaCl₂, and a stirring speed of 500 rpm. Around 22 % sludge disruption has been obtained. Alternating polarity has been shown to effectively prevent electrode passivation, achieving a 25 % improvement in sludge disruption. The treated sludge solution was then reintroduced into the SBR for metabolic assimilation. The results indicate that the SBR process can maintain a self-sustaining condition with up to 10 % sludge recirculation volume. The study provides new insights into in situ sludge reduction, with promise for widespread application and further optimization in future wastewater treatment systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135135"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"phenolic compounds promote humification by inducing functional focus in microbial communities","authors":"Xinying Yang,Yuchen Zhang,Xiangdi Wu,Qingsong Jia,Yuanzheng Sun,Junqiu Wu","doi":"10.1016/j.biortech.2026.135993","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135993","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"22 1","pages":"135993"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alanine aminotransferase–acetyl-CoA carboxylase switching produces starch-rich bioethanol feedstock in Desmodesmus","authors":"Jen-Chih Chen,Jo-Shu Chang,Tse-Min Lee","doi":"10.1016/j.biortech.2026.135992","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135992","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"37 1","pages":"135992"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic rewiring of Synechococcus elongatus PCC 7942 enhances astaxanthin production from CO2","authors":"Jinju Ma,Yulin Cui,Chunxiao Meng,Fengjie Sun,Lianshi Li,Yuanjie Zeng,Ze Chen Xin,Meiling Dan,Xingyue Ma,Hao Zhang,Guofu Chen,Zhengquan Gao","doi":"10.1016/j.biortech.2026.136006","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136006","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"7 1","pages":"136006"},"PeriodicalIF":11.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing pyrite-based bioretention systems under complex stormwater conditions using a mixed carbon source strategy: Insights into complementary carbon release, microbial restructuring, and pyrite regeneration.","authors":"Hao Zheng, Haiyuan Ma, Zheng Kong, Wenlin Zhao, Xinyue Wang, Huan Xiao, Yuanxiang Mao, Shihu Hu, Hongxiang Chai","doi":"10.1016/j.biortech.2026.135181","DOIUrl":"10.1016/j.biortech.2026.135181","url":null,"abstract":"<p><p>Pyrite-based bioretention systems hold substantial promise for the simultaneous removal of nitrogen and phosphorus from stormwater runoff. However, their performance under high-frequency and high-intensity rainfall conditions is often limited by the low electron-supply capacity of pyrite. To address this limitation, a mixed carbon source strategy was developed to enhance nutrient removal under complex rainfall conditions. Four bioretention systems, including the mixed carbon source-pyrite system (WCP), woodchip-pyrite system, corncob-pyrite system, and pyrite-only system, were constructed and systematically evaluated under simulated rainfall events with varying intensities and frequencies. Among all systems, WCP consistently achieved the best performance, with removal efficiencies of 88.1 ± 1.3% for ammonium, 86.6 ± 1.9% for nitrate, 87.0 ± 2.0% for total dissolved nitrogen, and 83.3 ± 4.3% for total dissolved phosphorus. Mechanistic analyses revealed that readily biodegradable carbon released from corncob promoted rapid heterotrophic denitrification during rainfall events, whereas recalcitrant carbon released from woodchips sustained pyrite-driven autotrophic denitrification during drying periods. Their complementary carbon-release characteristics synergistically enhanced nitrogen and phosphorus removal. In addition, mixed carbon sources reshaped the microbial community, enhanced cooperation between autotrophic and heterotrophic functional groups, and improved nitrogen transformation and electron transfer efficiency. Media characterization further showed that mixed carbon sources promoted FeS/FeS₂ cycling, thereby facilitating pyrite regeneration and supporting long-term operational stability. Overall, this study elucidates a multi-dimensional enhancement mechanism driven by mixed carbon sources-complementary carbon release, microbial restructuring, and pyrite regeneration-and provides a robust and sustainable strategy for nutrient control in stormwater bioretention systems under complex rainfall conditions.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135181"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148256618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}