Bioresource Technology最新文献_第9页

Modulating phosphate transfer process for promoting phosphorylation activity of acid phosphatase

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-11 DOI: 10.1016/j.biortech.2025.132348

Kai Linghu , Kangjie Xu , Xinyi Zhao , Jingwen Zhou , Xinglong Wang

{"title":"Modulating phosphate transfer process for promoting phosphorylation activity of acid phosphatase","authors":"Kai Linghu , Kangjie Xu , Xinyi Zhao , Jingwen Zhou , Xinglong Wang","doi":"10.1016/j.biortech.2025.132348","DOIUrl":"10.1016/j.biortech.2025.132348","url":null,"abstract":"<div><div><em>Klebsiella pneumonia</em> acid phosphatase is widely employed in the large-scale synthesis of nucleotides. It was found that the phosphate acceptance capability of the substrate limited the efficiency of the phosphate transfer process. By reducing steric hindrance and optimizing substrate interaction with the catalytic site, variants of <em>Klebsiella pneumonia</em> acid phosphatase were designed, with the E104G variant showing significantly enhanced hydrolysis activity while maintaining high phosphorylation activity. Crystal structure and quantum mechanics/molecular mechanics analyses of the E104G variant revealed that the mutation promotes substrate binding and lowers the energy barrier. Based on these insights, several mutations were designed, achieving significantly improved conversion rates. By knocking out degradation-related enzymes, the degradation rates of inosinic acid and guanylic acid were successfully controlled. This study provides a structure-based top-down design strategy that effectively enhances enzyme specificity, offering a promising enzyme candidate for large-scale nucleotide synthesis.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"427 ","pages":"Article 132348"},"PeriodicalIF":9.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diluted swine and aquaculture wastewater enhance carbon sequestration and nutrient removal by the red seaweed Agardhiella subulata

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-11 DOI: 10.1016/j.biortech.2025.132327

W.Sanjaya Weerakkody , Hsueh-Han Hsieh , Vicente G Abedneko , Ka Hin Ling , Tzu-Chieh Chung , Zih-Yi Wang , Yung-Yen Shih , Tse-Min Lee , Chen Xu , Peter H. Santschi , Chin-Chang Hung

{"title":"Diluted swine and aquaculture wastewater enhance carbon sequestration and nutrient removal by the red seaweed Agardhiella subulata","authors":"W.Sanjaya Weerakkody , Hsueh-Han Hsieh , Vicente G Abedneko , Ka Hin Ling , Tzu-Chieh Chung , Zih-Yi Wang , Yung-Yen Shih , Tse-Min Lee , Chen Xu , Peter H. Santschi , Chin-Chang Hung","doi":"10.1016/j.biortech.2025.132327","DOIUrl":"10.1016/j.biortech.2025.132327","url":null,"abstract":"<div><div>Swine wastewater poses significant environmental challenges, and conventional microalgae treatments often leave high residual nutrients while consuming substantial freshwater. This study explored the potential of the saltwater macroalgae <em>Agardhiella subulata</em> for nutrient removal and carbon assimilation under varying salinity and ammonium concentrations. Using diluted swine and aquaculture wastewater, <em>A. subulata</em> achieved ammonium and phosphorus removal efficiencies of up to 93 % and 68 %, respectively, within 24 h. It also removed CO<sub>2</sub> at rates five times higher than the global forest average and enhanced dissolved oxygen levels, reducing the environmental impact of nutrient-rich wastewater while minimizing freshwater demand. Notably, <em>A. subulata</em> effectively utilized swine wastewater with ammonia concentrations up to 600 μM under salinity conditions of 27–34 ppt. These results demonstrate the potential of <em>A. subulata</em> for large-scale nutrient removal and CO<sub>2</sub> reduction, providing a sustainable solution to the environmental impacts of the livestock industry.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"427 ","pages":"Article 132327"},"PeriodicalIF":9.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced nitrogen prediction and mechanistic process analysis in high-salinity wastewater treatment using interpretable machine learning approach

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-11 DOI: 10.1016/j.biortech.2025.132393

Qing Wei , Zuxin Xu , Hailong Yin

{"title":"Enhanced nitrogen prediction and mechanistic process analysis in high-salinity wastewater treatment using interpretable machine learning approach","authors":"Qing Wei , Zuxin Xu , Hailong Yin","doi":"10.1016/j.biortech.2025.132393","DOIUrl":"10.1016/j.biortech.2025.132393","url":null,"abstract":"<div><div>This study introduces an interpretable machine learning framework to predict nitrogen removal in membrane bioreactor (MBR) treating high-salinity wastewater. By integrating Shapley additive explanations (SHAP) with Categorical Boosting (CatBoost), we address the critical gap in linking predictive accuracy to operational decision-making for saline systems. CatBoost achieved the best performance, with an coefficient of determination (R<sup>2</sup>) of 0.88 and root mean square error (RMSE) of 4.27 for the effluent ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N<sub>out</sub>), and an R<sup>2</sup> of 0.91 and RMSE of 4.35 for the effluent total nitrogen (TN<sub>out</sub>). SHAP analysis uniquely revealed salinity’s dual role in inhibiting nitrifying enzymes and disrupting carbon metabolism, with dissolved oxygen, pH and chemical oxygen demand removal efficiency as key regulators. Temperature and carbon-to-nitrogen ratio further modulated total nitrogen dynamics through electron donor availability and microbial activity. The proposed SHAP-CatBoost model in high salinity MBR combines predictive modelling with mechanical process control.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132393"},"PeriodicalIF":9.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on adhesion and irreversible fouling to delineate mitigation effects of autoinducer-2 mediated quorum quenching on biofouling

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-10 DOI: 10.1016/j.biortech.2025.132380

Yuan Li, Danchen Zhu, Linmeng Hong, Jixiang Dai, Lijun Wu

{"title":"Perspectives on adhesion and irreversible fouling to delineate mitigation effects of autoinducer-2 mediated quorum quenching on biofouling","authors":"Yuan Li, Danchen Zhu, Linmeng Hong, Jixiang Dai, Lijun Wu","doi":"10.1016/j.biortech.2025.132380","DOIUrl":"10.1016/j.biortech.2025.132380","url":null,"abstract":"<div><div>This study systematically investigated the effect of interfering with autoinducer-2 (AI-2) signaling system on controlling initial fouling attachment and irreversible components. Exposure of bacteria to the inhibitor significantly reduced the expression of the luxS gene, which resulted in a 37.3% reduction of AI-2 signaling molecule synthesis. Expression of flagellin and kinesin, which determine bacteria adhesion properties, and virulence genes was significantly down-regulated. AI-2 quorum quenching (QQ) significantly suppressed the initial biofilm growth and inhibition efficiency was positively correlated with bacteria concentration. Extracellular polymeric substances accumulation decreased by 40.8% in QQ experiment relative to the control, thus mitigating the flux decline. Polysaccharides, which constitute irreversible components, were significantly reduced by 69.3% with presence of inhibitor. AI-2 QQ altered protein and peptide structure of fouling layer, which converted irreversible fouling into reversible fouling. These findings contributed to the understanding of QQ susceptibility to initial fouling, providing a theoretical basis for engineering applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132380"},"PeriodicalIF":9.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alkaline-thermal synergistic activation of persulfate for sawdust hour-level humification to prepare fulvic-like-acid fertilizer

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-10 DOI: 10.1016/j.biortech.2025.132388

Dongfang Wang , Xinyan Chen , Jing Zhang , Jia Xu , Xianghai Kong , Jinghong Ye , Rongjun Zhang , Huiqun Fan , Li Liu , Xiuping Zhan , Yan Qin , He Xu , Yanping Zhu , Dongqing Cai

{"title":"Alkaline-thermal synergistic activation of persulfate for sawdust hour-level humification to prepare fulvic-like-acid fertilizer","authors":"Dongfang Wang , Xinyan Chen , Jing Zhang , Jia Xu , Xianghai Kong , Jinghong Ye , Rongjun Zhang , Huiqun Fan , Li Liu , Xiuping Zhan , Yan Qin , He Xu , Yanping Zhu , Dongqing Cai","doi":"10.1016/j.biortech.2025.132388","DOIUrl":"10.1016/j.biortech.2025.132388","url":null,"abstract":"<div><div>Sawdust is a by-product of wood processing and it was rapidly humified with K<sub>2</sub>S<sub>2</sub>O<sub>8</sub> under alkaline-thermal synergistic activation to produce a fulvic-like-acid (FLA) organic fertilizer (SFOF) in this study. The optimum conditions were K<sub>2</sub>S<sub>2</sub>O<sub>8</sub>: KOH mass ratio of 1:2 and 150°C, meanwhile FLA yield could reach 180.3 mg/g in 2 h. The carboxylation, Maillard reaction, and aromatization processes occurred during sawdust humification. And then, SFOF was mixed with attapulgite and modified starch binder to get an organic fertilizer (SAM), and coated with amino silicone oil (ASO) to create a slow-release granule (SAM@ASO). The release mechanism of FLA from SAM@ASO was consistent with Ritger-Peppas release kinetics. SAM@ASO, with high biosafety, could promote water spinach growth and remediate acidic soil (pH from 4.9 to 6.3). This method offers a promising approach for sawdust utilization and a novel FLA-based organic fertilizer for acidic soil remediation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132388"},"PeriodicalIF":9.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced dissimilatory nitrate reduction to ammonium and electron transfer mechanisms in bidirectional electron transfer biofilm constructed by iron phthalocyanine

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-10 DOI: 10.1016/j.biortech.2025.132381

Lei He , Xuejie He , Ying Zhang , Xing Fan , Tao Yang , Xiaopeng Ji , Yingmu Wang , Jian Zhou , Chengbao Lin

{"title":"Enhanced dissimilatory nitrate reduction to ammonium and electron transfer mechanisms in bidirectional electron transfer biofilm constructed by iron phthalocyanine","authors":"Lei He , Xuejie He , Ying Zhang , Xing Fan , Tao Yang , Xiaopeng Ji , Yingmu Wang , Jian Zhou , Chengbao Lin","doi":"10.1016/j.biortech.2025.132381","DOIUrl":"10.1016/j.biortech.2025.132381","url":null,"abstract":"<div><div>Bidirectional electron transfer biofilms (BETB) could efficiently reduce nitrate without accumulating nitrite, representing a promising biological electrochemical denitrification technology. This study utilized iron phthalocyanine modified carbon felt (FePc-CF) to enrich electroactive bacteria, constructing a long-term stable FePc-BETB. Its nitrate removal rate reached 91%, far exceeding the traditional nitrate-reducing biocathode (45%) and Con-BETB (46%). The dissimilatory nitrate reduction to ammonium (DNRA) dominated nitrate reduction in FePc-BETB, consuming 35% of the total electrons. Additionally, FePc-BETB effectively reduced the accumulation of NO<sub>2</sub><sup>−</sup>-N and N<sub>2</sub>O. Electrochemical analysis demonstrated FePc-BETB exhibited stronger electrochemical activity and electron transfer capability. Mediated electron transfer (MET) enhanced by increased extracellular humic acid in FePc-BETB favored the electron supplement for nitrate removal. The relative abundance of <em>nrfA</em>, marker of the DNRA, increased significantly. This study provided new insights into regulating denitrification and DNRA pathways and treating nitrate wastewater lacking electron donors.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132381"},"PeriodicalIF":9.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid profiles of green conversion from corn-ethanol co-product via Aspergillus niger

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-10 DOI: 10.1016/j.biortech.2025.132384

Weiwei Fan , Ayong Wang , Xiaoying Che , Siyu Xu , Ming Chen , Zhanyou Chi

{"title":"Lipid profiles of green conversion from corn-ethanol co-product via Aspergillus niger","authors":"Weiwei Fan , Ayong Wang , Xiaoying Che , Siyu Xu , Ming Chen , Zhanyou Chi","doi":"10.1016/j.biortech.2025.132384","DOIUrl":"10.1016/j.biortech.2025.132384","url":null,"abstract":"<div><div>High-value recycling of agro-industrial by-products is the focus of global sustainable development. A method of the recovery and utilization of corn-ethanol co-product to produce functional lipids via <em>Aspergillus niger</em> (<em>A. niger</em>) was proposed. The lipid changes in distillers dried grains with solubles (DDGS) were monitored via lipidomics. 648 lipids (five classes, 29 subclasses) were identified, including 75 fatty acyls, 203 glycerolipids, 184 glycerophospholipids, 169 sphingolipids, and 17 glucosylsphingoshine. Glycerolipids were the most abundant lipids, accounting for 31%. As fermentation proceeded, the concentration of lipids with 1–9 unsaturated bonds steadily increased. Oleic acid and linoleic acid were the main accumulated fatty acids. The pathways enrichment results showed glycerophospholipid metabolism, glycerolipids metabolism, sphingolipid metabolism, and biosynthesis of unsaturated fatty acids were the key metabolic pathways involved during DDGS fermentation. These results provided a comprehensive knowledge of the lipid profiles in fermented DDGS and proposed a new approach for high-value utilization of DDGS.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132384"},"PeriodicalIF":9.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of N-acyl homoserine lactones secretion in short-chain fatty acid co-metabolic methane production system influenced by ecological factors and its microbiological mechanisms

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-09 DOI: 10.1016/j.biortech.2025.132382

Liyan He, Gefu Zhu

{"title":"Characteristics of N-acyl homoserine lactones secretion in short-chain fatty acid co-metabolic methane production system influenced by ecological factors and its microbiological mechanisms","authors":"Liyan He, Gefu Zhu","doi":"10.1016/j.biortech.2025.132382","DOIUrl":"10.1016/j.biortech.2025.132382","url":null,"abstract":"<div><div>The effective enhancement of short-chain fatty acid co-metabolic methane production is a research hotspot. <em>N</em>-acyl homoserine lactones (AHLs) are effective means regulating anaerobic digestion behaviors. However, what factors influence the secretion of endogenous signaling molecules has not been studied. We established a propionic acid co-metabolic methane production system to examined how ecological factors affect AHLs secretion. We found that propionic acid concentration negatively correlated with C4-HSL secretion (-0.71 for <em>R</em>). Ammonia nitrogen positively correlated with C4-HSL secretion at low 100–1000 mg/L (0.89 for <em>R</em>), and negatively correlated at 1000–6000 mg/L (-0.95 for <em>R</em>). While pH positively correlated with C6-HSL (0.76 for <em>R</em>). <em>Syntrophobacter</em>, <em>Syntrophorhabdus</em>, and <em>Methanospirillum</em> significantly affected C4-HSL secretion. We also demonstrated a significant correlation between signaling molecules, syntrophic bacteria, hydrogenotrophic methanogens, and maximum methane production. Our study provided a new understanding for the future research of using AHLs to regulate the metabolism of syntrophic bacteria for methane production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132382"},"PeriodicalIF":9.7,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remediation of wastewater by using CdS-based biohybrids: Challenges and enhancement strategies

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-08 DOI: 10.1016/j.biortech.2025.132379

Yilin Le, Huilei Wang

{"title":"Remediation of wastewater by using CdS-based biohybrids: Challenges and enhancement strategies","authors":"Yilin Le, Huilei Wang","doi":"10.1016/j.biortech.2025.132379","DOIUrl":"10.1016/j.biortech.2025.132379","url":null,"abstract":"<div><div>Co-existence of pollutants, specifically heavy metals and organic compounds, as well as multi-heavy metals, in wastewater presents a significant global environmental and public health concern. The combined presence of these pollutants can result in a synergistic increase in toxicity, making the simultaneous removal of heavy metals and organic contaminants a complex challenge. Cadmium sulfide-based photocatalyst-microbe biohybrids, which integrate the advantages of whole-cell biological catalysts and semiconducting nanomaterials, have garnered considerable interest. These biohybrids offer benefits such as the capacity for heavy metal ion recycling, enhanced remediation efficiency, and the simultaneous removal of combined pollutants. This review begins by outlining the strategy for developing CdS-based biohybrid systems. Subsequently, it explores the application and mechanisms of remediation facilitated by these systems. The current challenges and future perspectives for biohybrid systems are also discussed. Finally, the review highlights the potential of synthetic biology strategies for optimizing and constructing biohybrid systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"426 ","pages":"Article 132379"},"PeriodicalIF":9.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medium chain fatty acid production from CO2 in integrated dark fermentation-microbial electrosynthesis reactor

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-08 DOI: 10.1016/j.biortech.2025.132371

Narnepati Krishna Chaitanya , Pritha Chatterjee

引用次数: 0