Bioresource Technology最新文献_第9页

Comparison of the performances and mechanisms of anammox bacteria in-situ self-enrichment under heterotrophic and autotrophic conditions by inoculating ordinary activated sludge

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-11 DOI: 10.1016/j.biortech.2025.132213

Zhaoxu Peng , Jing Qin , Ying Zhao , Ying Li , Feiyue Hu , Zhixuan Bai , Haosen Yang , Baodan Jin , Jiantao Ji

{"title":"Comparison of the performances and mechanisms of anammox bacteria in-situ self-enrichment under heterotrophic and autotrophic conditions by inoculating ordinary activated sludge","authors":"Zhaoxu Peng , Jing Qin , Ying Zhao , Ying Li , Feiyue Hu , Zhixuan Bai , Haosen Yang , Baodan Jin , Jiantao Ji","doi":"10.1016/j.biortech.2025.132213","DOIUrl":"10.1016/j.biortech.2025.132213","url":null,"abstract":"<div><div>The in-situ self-enrichment of anammox bacteria (AnAOB) was a pivotal facet of anammox in mainstream wastewaters treatment with low ammonia strength. This study explored the performances and mechanisms of AnAOB in-situ self-enrichment under heterotrophic and autotrophic conditions. The absolute abundances of AnAOB increased from 1.7 × 10<sup>5</sup> to 1.6 × 10<sup>9</sup> copies/g dry sludge in the partial denitrification/anammox (PD/A) system and 4.6 × 10<sup>8</sup> copies/g dry sludge in the single anammox system, and the corresponding anammox activities reached 42.7 and 6.5 mg NH<sub>4</sub><sup>+</sup>-N/g VSS/d. In the PD/A system, denitrifying bacteria secreted abundant signal molecules to communicate with AnAOB, thereby promoting the high self-enrichment of AnAOB. Accordingly, the PD/A system showed significant advantages on AnAOB self-enrichment compared with the single anammox system. This study provided new insights into the mechanism of cooperative and competitive interactions between PD bacteria and AnAOB during the AnAOB self-enrichment, promoting the application of anammox process in low-ammonia wastewaters treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"422 ","pages":"Article 132213"},"PeriodicalIF":9.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peroxisomal biogenesis factor 11 as a novel target to trigger lipid biosynthesis and salt stress resistance in oleaginous Tetradesmus obliquus

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-10 DOI: 10.1016/j.biortech.2025.132209

Yunzhuan Xue , Wei Wang , Xiajule Sheng , Zexu Zheng , Zihan Wang , Fangling Ding , Jinjin Li , Zhiwei Sun , Yu’ang Cai , Xianhua Wang , Jiao Xue

{"title":"Peroxisomal biogenesis factor 11 as a novel target to trigger lipid biosynthesis and salt stress resistance in oleaginous Tetradesmus obliquus","authors":"Yunzhuan Xue , Wei Wang , Xiajule Sheng , Zexu Zheng , Zihan Wang , Fangling Ding , Jinjin Li , Zhiwei Sun , Yu’ang Cai , Xianhua Wang , Jiao Xue","doi":"10.1016/j.biortech.2025.132209","DOIUrl":"10.1016/j.biortech.2025.132209","url":null,"abstract":"<div><div>To overcome economic challenges in microalgal biofuel production, this study investigates the overexpression of peroxisome-localized peroxisomal biogenesis factor 11 (PEX11) to enhance lipid biosynthesis and improve salt stress resistance in <em>Tetradesmus obliquus</em>, aiming to advance microalgal biofuel production. Transgenic strains PEX11-2-1 and PEX11-2-2 exhibited a 2.13- and 2.51-fold increase in neutral lipid content and more cellular lipid droplets compared to WT, along with lipid yield and biomass escalating to 255.45 and 815.15 mg/L, respectively. This enhancement resulted from the redistribution of carbon precursors, increased intracellular reactive oxygen species, enhanced NADPH synthesis, and upregulation of lipid synthesis genes. Additionally, PEX11 improved salt stress tolerance by upregulating the expression of stress-responsive genes, including <em>SnRK2</em> and <em>PYRC</em>. Fatty acid profile alterations, with increases in saturated fatty acids C16:0 and monounsaturated fatty acids C18:1, and decreases in polyunsaturated fatty acids, facilitated high-quality biofuel production. These findings highlight novel insights for advancing microalgae-based biorefinery.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132209"},"PeriodicalIF":9.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of Tween 20 and ethephon on yeast oil and β-carotene co-production by Rhodosporidium toruloides using purified biodiesel-derived crude glycerol as an alternative carbon source

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-10 DOI: 10.1016/j.biortech.2025.132211

Sasitorn Khuntong , Thidarat Samranrit , Parichat Koedprasong , Jantima Teeka , Chiu-Hsia Chiu , Witsanu Srila , Atsadawut Areesirisuk

{"title":"Synergistic effects of Tween 20 and ethephon on yeast oil and β-carotene co-production by Rhodosporidium toruloides using purified biodiesel-derived crude glycerol as an alternative carbon source","authors":"Sasitorn Khuntong , Thidarat Samranrit , Parichat Koedprasong , Jantima Teeka , Chiu-Hsia Chiu , Witsanu Srila , Atsadawut Areesirisuk","doi":"10.1016/j.biortech.2025.132211","DOIUrl":"10.1016/j.biortech.2025.132211","url":null,"abstract":"<div><div>This study investigated the impact of chemical inducers on the co-production of yeast oil (YO) and β-carotene from purified biodiesel-derived crude glycerol. The objective was to enhance YO and β-carotene co-production in <em>Rhodosporidium toruloides</em> through the application of individual and combined inducers at both flask and bioreactor scales. Among the individual inducers, 1 % w/v Tween 20 (TW) and 10 ppm ethephon (EP) significantly increased total yeast oil (TO) and total β-carotene (TC) concentrations, respectively. When TW and EP were used together, TO and TC production increased by 2.0 and 2.6-fold, respectively in the bioreactor compared to the flask. The YO primarily consisted of C16 and C18 long-chain fatty acids, and the β-carotene produced showed functional similarities to commercial β-carotene. This research highlights the potential of biodiesel waste as a sustainable feedstock for co-producing YO and β-carotene, with the dual-inducer strategy providing a simple and effective method for enhancing production efficiency.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"422 ","pages":"Article 132211"},"PeriodicalIF":9.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced nitrogen removal from extremely low carbon/nitrogen ratio municipal wastewater by optimizing multiple pathways based on step-feed and intermittent aeration

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-09 DOI: 10.1016/j.biortech.2025.132202

Yanru Cui , Liang Zhang , Hanbin Wang , Xinsheng Fan , Yongzhen Peng

{"title":"Advanced nitrogen removal from extremely low carbon/nitrogen ratio municipal wastewater by optimizing multiple pathways based on step-feed and intermittent aeration","authors":"Yanru Cui , Liang Zhang , Hanbin Wang , Xinsheng Fan , Yongzhen Peng","doi":"10.1016/j.biortech.2025.132202","DOIUrl":"10.1016/j.biortech.2025.132202","url":null,"abstract":"<div><div>The advantages of step-feed and intermittent aeration have been well-documented, however, combining them to enhance nitrogen removal in anaerobic/oxic/anoxic systems has been rarely explored. This study established the non-single-form anoxic stages by step-feed and intermittent aeration and finally enhanced the nitrogen removal of real municipal wastewater with step-feed anaerobic/oxic/anoxic/oxic/anoxic operation mode. Results revealed that <em>Candidatus_Brocadia</em> increased from 0.00 % to 0.21 % in the suspended sludge system, contributing 54.7 % of the nitrogen removal. Partial nitrification (PN) and endogenous partial denitrification (EPD) supplied nitrite for Anammox. A comprehensive multi-pathway nitrogen removal system, encompassing PN, nitrification, partial denitrification, Anammox, EPD/Anammox, and denitrification was constructed. The system effectively reduced total inorganic nitrogen concentration to 3.6 ± 1.2 mg/L at a carbon/nitrogen ratio of 3.0 ± 0.3, achieving a nitrogen removal efficiency of 95.3 ± 1.5 %. This study provides a novel approach for the advanced treatment of municipal wastewater and enrichment of anaerobic ammonia-oxidizing bacteria.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132202"},"PeriodicalIF":9.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of caproate production via carboxylate chain elongation with sequential fermentation facilitated by biochar: A corn stover full-component utilization perspective

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-09 DOI: 10.1016/j.biortech.2025.132208

Xiwen Jia , Dong Liu , Jiajie Xu , Hui Zhang , Luoyang Liu , Lujia Han , Weihua Xiao , Suan Shi

{"title":"Enhancement of caproate production via carboxylate chain elongation with sequential fermentation facilitated by biochar: A corn stover full-component utilization perspective","authors":"Xiwen Jia , Dong Liu , Jiajie Xu , Hui Zhang , Luoyang Liu , Lujia Han , Weihua Xiao , Suan Shi","doi":"10.1016/j.biortech.2025.132208","DOIUrl":"10.1016/j.biortech.2025.132208","url":null,"abstract":"<div><div>In this study, caproate was synthesized from corn stover through sequential fermentation, and biochar was prepared from unhydrolyzable corn stover by pyrolysis to utilize full-component of corn stover. The results indicate that the caproate concentration in the unhydrolyzable corn stover biochar (UCSB) group was 2.2 times higher than that of the control group, and the fermentation start-up time was shortened by 18 days. Mechanistic analysis suggested that the rough surface of UCSB facilitated microbial colonization and reduced product inhibition. Genes expression analysis further demonstrated that UCSB significantly upregulated crucial functional genes responsible for ethanol oxidation and the reverse <em>β</em>-oxidation pathway, ultimately resulting in enhanced caproate production. The successful utilization of UCSB derived from unhydrolyzable solid residue effectively boosted fermentation, leading to a 37 % increase in the carbon utilization efficiency of corn stover. This study offering valuable insights for the high-value and full-component utilization of lignocellulosic biomass.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132208"},"PeriodicalIF":9.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bayesian Optimization-Enhanced Reinforcement learning for Self-adaptive and multi-objective control of wastewater treatment

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-09 DOI: 10.1016/j.biortech.2025.132210

Ziang Zhu , Shaokang Dong , Han Zhang , Wayne Parker , Ran Yin , Xuanye Bai , Zhengxin Yu , Jinfeng Wang , Yang Gao , Hongqiang Ren

{"title":"Bayesian Optimization-Enhanced Reinforcement learning for Self-adaptive and multi-objective control of wastewater treatment","authors":"Ziang Zhu , Shaokang Dong , Han Zhang , Wayne Parker , Ran Yin , Xuanye Bai , Zhengxin Yu , Jinfeng Wang , Yang Gao , Hongqiang Ren","doi":"10.1016/j.biortech.2025.132210","DOIUrl":"10.1016/j.biortech.2025.132210","url":null,"abstract":"<div><div>Controllers of wastewater treatment plants (WWTPs) often struggle to maintain optimal performance due to dynamic influent characteristics and the need to balance multiple operational objectives. In this study, Reinforcement Learning (RL) algorithms across different activated sludge process configurations was tested, and a novel approach that integrates RL with Bayesian Optimization (BO) to enhance the control of critical operational parameters in activated sludge processes was developed. This study extended the application of advanced machine learning techniques to complex WWTP control problems, moving beyond simplified benchmarks. The integration of BO with RL avoided sub-optimal performance and accelerated convergence to optimal control policies in controlling the A2O process, resulting in a significant 46% reduction in operational costs and a 12% decrease in energy consumption while maintaining compliance with effluent discharge standards. This approach offers a practical pathway for WWTPs to enhance treatment efficiency, reduce operational costs, and contribute to sustainable wastewater management practices.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132210"},"PeriodicalIF":9.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation pattern of carotenoid biosynthesis induced by low light stress in Dunaliella

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-08 DOI: 10.1016/j.biortech.2025.132206

Hao-Hong Chen , Jing-Xuan Wu , Rui-qi Chen , Sacha Fouquay O’Donnell , Ming-Hua Liang , Jian-Guo Jiang

{"title":"Regulation pattern of carotenoid biosynthesis induced by low light stress in Dunaliella","authors":"Hao-Hong Chen , Jing-Xuan Wu , Rui-qi Chen , Sacha Fouquay O’Donnell , Ming-Hua Liang , Jian-Guo Jiang","doi":"10.1016/j.biortech.2025.132206","DOIUrl":"10.1016/j.biortech.2025.132206","url":null,"abstract":"<div><div>This research aims to explore the response mechanism of carotenoid synthesis in <em>Dunaliella bardawil</em> under low light stress (≤13.5 µmol·m<sup>−2</sup>·s<sup>−1</sup>). We analyzed carotenoid composition and its correlation with carotenogenic genes (<em>CRTs</em>) differential expression, promoter regulatory elements and transcription factors. <em>DbPDS</em> and <em>DbZDS</em> were significantly up-regulated under low light stress. Mutation experiments showed ACE, ASF1, CCACA-box, and CCAAT-box in <em>DbZDS</em> promoter key for low light regulation. In addition, CONSTANS (DbCO) and nuclear transcription factor YB (DbNF-YB) had a weak interaction and could bind to <em>DbZDS</em> gene promoter, whereas there was no interaction between <em>DbZDS</em> promoter and DbHY5 (ELONGATED HYPOCOTYL 5). We constructed a <em>DbCO</em> gene overexpressing strain, which significantly enhanced carotenoid content, notably increasing <em>β</em>-carotene levels to 2.3 times. These results significantly enhance our understanding of the regulation of low light stress in microalgae.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132206"},"PeriodicalIF":9.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing biofilm thickness, density, and microbial community composition in a full-scale hybrid membrane aerated biofilm reactor

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-08 DOI: 10.1016/j.biortech.2025.132207

Narasimman Lakshminarasimman , Michelle M. McKnight , Josh D. Neufeld , Wayne Parker

{"title":"Characterizing biofilm thickness, density, and microbial community composition in a full-scale hybrid membrane aerated biofilm reactor","authors":"Narasimman Lakshminarasimman , Michelle M. McKnight , Josh D. Neufeld , Wayne Parker","doi":"10.1016/j.biortech.2025.132207","DOIUrl":"10.1016/j.biortech.2025.132207","url":null,"abstract":"<div><div>This study examined biofilm thickness, density, and microbial composition in a full-scale MABR treating municipal wastewater, focusing on their spatial and operational variability. The MABR cassette arrangement created a thickness gradient, with biofilms in the front cassettes more than twice as thick as those at the back. Lower scouring intensity due to reduced airflow resulted in thicker biofilms. Microbial communities varied longitudinally and by operational phase, with thicker biofilms having a higher relative abundance of anaerobic microorganisms, such as fermenters and sulfur reducers, and fewer aerobic nitrifiers. <em>Nitrosomonas</em> were the main ammonia oxidizers, while <em>Nitrospira</em> and <em>Ca.</em> Nitrotoga dominated as nitrite oxidizers. The 16S RNA gene profiles showed strong correlations with biofilm thickness (R<em><sup>2</sup></em> = 0.8) and nitrification rates (R<em><sup>2</sup></em> = 0.4). Full-scale MABR biofilm characteristics have not been studied before. Study findings have practical implications for better modeling practices and improved design of future MABR facilities.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"423 ","pages":"Article 132207"},"PeriodicalIF":9.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient biodegradation of flurochloridone herbicide by Streptomyces parvus strain F-G-2 and its degradation mechanism

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-08 DOI: 10.1016/j.biortech.2025.132158

Dong Zhao, Shuo Shen, Junlong Feng, Shuqun Deng, Enyu Lu, Chenxu Gao, Yang Zhang, Hongyu Chen, Wei Li

{"title":"Efficient biodegradation of flurochloridone herbicide by Streptomyces parvus strain F-G-2 and its degradation mechanism","authors":"Dong Zhao, Shuo Shen, Junlong Feng, Shuqun Deng, Enyu Lu, Chenxu Gao, Yang Zhang, Hongyu Chen, Wei Li","doi":"10.1016/j.biortech.2025.132158","DOIUrl":"10.1016/j.biortech.2025.132158","url":null,"abstract":"<div><div>Flurochloridone (FLC) is a pyrrolidone herbicide with good weed control effects. However, it poses potential threats to the ecological environment and human health. To alleviate the impact of FLC residues, a bacterium capable of degrading FLC, <em>Streptomyces</em> sp. F-G-2, was isolated from FLC-contaminated soil. Within 42 days (d), the strain F-G-2 metabolizes 80.0 % of FLC, there is a significant selective degradation trend towards the enantiomers (−)-(3<em>S</em>, 4<em>S</em>)-FLC and (+)-(3<em>R</em>, 4<em>R</em>)-FLC in degradation. The strain’s introduction into soil does not affect the structure and diversity of the indigenous microbial community. Two enzymes responsible for FLC degradation in the strain F-G-2 were identified: <em>α/β</em> hydrolase and 2-hydroxy acid dehydrogenase, with key residues ILE-14 and LYS-187 playing a role. LC-MS/MS analysis confirmed FLC hydrolysis through carbonyl bonds. These findings enhance our understanding of the molecular mechanisms of FLC biodegradation and provide a theoretical basis for environmental remediation in the Qinghai-Tibet Plateau (QTP).</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132158"},"PeriodicalIF":9.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the effects of acid/alkali-catalyzed glycerol organosolv pretreatment on lignocellulosic biorefinery: Substrate digestibility and high-quality lignin

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-02-07 DOI: 10.1016/j.biortech.2025.132203

Lingfeng Long , Dongqin He , Tao Zhang , Xiaoxiao Chen , Xianzhi Meng , Arthur J. Ragauskas , Chen Huang

{"title":"Unlocking the effects of acid/alkali-catalyzed glycerol organosolv pretreatment on lignocellulosic biorefinery: Substrate digestibility and high-quality lignin","authors":"Lingfeng Long , Dongqin He , Tao Zhang , Xiaoxiao Chen , Xianzhi Meng , Arthur J. Ragauskas , Chen Huang","doi":"10.1016/j.biortech.2025.132203","DOIUrl":"10.1016/j.biortech.2025.132203","url":null,"abstract":"<div><div>This work systematically assessed three acid- or alkali-catalyzed glycerol (AGO/ALGO) pretreatments for lignocellulose fractionation into fermentable sugars and high-quality lignin. Results showed that compared with stand-alone process, catalyst-added pretreatments were more effective in delignification. AGO process had much higher hemicellulose removal selectivity than ALGO, resulting in a 32.7 % lower total sugar content in AGO solids compared to ALGO solids. It was also found that glyceryl lignin could increase cellulose hydrolysis yield, and ALGO lignin showed the highest improvement (35.9 %), which was beneficial to simplify the biorefinery route in the future. NMR analysis revealed that ALGO pretreatment was more favorable for lignin hydroxylation grafting reaction than AGO process, making recovered lignin have more <em>β-</em>O-4 linkages and aliphatic –OH groups. These results present pivotal insight into the design of organosolv pretreatment, aiming at platform sugar and lignin coproducts.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"421 ","pages":"Article 132203"},"PeriodicalIF":9.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0