Bioresource Technology最新文献_第9页

Synthetic coalification of microalgae through hydrothermal carbonization: strategies for enhanced hydrochar characteristics and technological advancements 微藻水热炭化合成煤化：提高烃类特性的策略及技术进步

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132542

Joydeepa Taran , Rajarshi Bhar , Hema Jha , Saikat Kumar Kuila , Biswajit Samal , Ranjan Pradhan , Brajesh Kumar Dubey

{"title":"Synthetic coalification of microalgae through hydrothermal carbonization: strategies for enhanced hydrochar characteristics and technological advancements","authors":"Joydeepa Taran , Rajarshi Bhar , Hema Jha , Saikat Kumar Kuila , Biswajit Samal , Ranjan Pradhan , Brajesh Kumar Dubey","doi":"10.1016/j.biortech.2025.132542","DOIUrl":"10.1016/j.biortech.2025.132542","url":null,"abstract":"<div><div>This review explores the hydrothermal carbonization (HTC) of microalgae through a comprehensive evaluation of the influence of process parameters on the resultant products. The findings revealed that HTC of microalgae takes place at lower temperatures (170 – 250 °C) compared to lignocellulosic feedstocks, and the resulting hydrochar and hydrolysate have a higher N-content. Additionally, secondary char production varies based on reaction conditions, with yields between 4 % and 35 %. The interaction between carbohydrates and nitrogenous compounds in the hydrolysate at varying reaction severities was discussed, underlining the extent of nitrogen fixation in the hydrochar and total organic C-content of up to 26.8 g L<sup>-1</sup>. The article also suggests strategies to improve hydrochar properties by assessing different technical strategies and emphasizing future direction research. In summary, this review underscores the potential of microalgal HTC as a sustainable approach for applications in energy and environmental applications via process optimization and technological upgradation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132542"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Feammox efficiency through riboflavin and humic acid: Nitrogen and iron transformation, energy metabolism, and microbial response 通过核黄素和腐植酸提高Feammox效率：氮和铁的转化、能量代谢和微生物反应

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132533

Dun Guo , Lei Yang , Hao-Qi Lu , Yu-Chao Wang , Hong-Yan Meng , Pan Liang , Shen Cui , Zhang-Wei He , Jun Lan , Yong-Xiang Ren

{"title":"Enhancing Feammox efficiency through riboflavin and humic acid: Nitrogen and iron transformation, energy metabolism, and microbial response","authors":"Dun Guo , Lei Yang , Hao-Qi Lu , Yu-Chao Wang , Hong-Yan Meng , Pan Liang , Shen Cui , Zhang-Wei He , Jun Lan , Yong-Xiang Ren","doi":"10.1016/j.biortech.2025.132533","DOIUrl":"10.1016/j.biortech.2025.132533","url":null,"abstract":"<div><div>Optimizing electron shuttles and revealing their mediating mechanisms are crucial for enhancing the ammonium (NH<sub>4</sub><sup>+</sup>-N) oxidation coupled with Fe (III) reduction. In this study, anthraquinone-2,6-disulfonate (AQDs), riboflavin (RF), and humic acid (HA) were optimized in batch tests. The optimal dosages of 6, 2, and 8 mg/L for AQDs, RF, and HA resulted in average maximum NH<sub>4</sub><sup>+</sup>-N removal of 80.2 %, 88.5 %, and 99.2 %, with 91.4 %, 88.5 %, and 74.7 % of the removed NH<sub>4</sub><sup>+</sup>-N converted to nitrate, respectively. In addition, an enhanced extracellular electron transfer was also observed, including an enlarged current, diversified REDOX pathway, and reduced resistance. Outperformed AQDs in nitrogen removal and microbial activity, HA and RF were selected for the subsequent 100-day long-term investigation. During this stage, excess influent Fe tended to be stored as insoluble coatings on the sludge surface, while RF and HA facilitated its use to compensate for the reduced influent Fe<sup>3+</sup>. Meanwhile, they led to an increase in iron-reducing (<em>Comamonas</em>) and NH<sub>4</sub><sup>+</sup>-N oxidizing bacteria (<em>Nitropsira</em> and <em>Planctomycetes</em>), as well as improvements in electrochemical characteristics and microbial activity. Moreover, microbial N and Fe metabolic potential were efficiently enhanced. Consequently, NH<sub>4</sub><sup>+</sup>-N and TN removal rates were obviously increased to approximately 90 % and 40 %, respectively. The addition of electron shuttles led to long-term improvements in extracellular mass transfer and microbial metabolism, which contributed more than bridging the extracellular electron transfer. These results deepened the understanding of the effect of electron shuttles on Feammox.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132533"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From waste to value: Multi-omics reveal pomegranate peel addition improves corn silage antioxidant activity and reduces methane and nitrogen losses 从废物到价值：多组学揭示石榴皮添加提高玉米青贮抗氧化活性，减少甲烷和氮的损失

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132544

Xia Zhang, Huixian Zhang, Dongcai Wang, Yuanqing Zhang

{"title":"From waste to value: Multi-omics reveal pomegranate peel addition improves corn silage antioxidant activity and reduces methane and nitrogen losses","authors":"Xia Zhang, Huixian Zhang, Dongcai Wang, Yuanqing Zhang","doi":"10.1016/j.biortech.2025.132544","DOIUrl":"10.1016/j.biortech.2025.132544","url":null,"abstract":"<div><div>Fermentation technology presents promising opportunities for food waste valorization. Pomegranate peel (<strong>PP</strong>), a food by-product, has potential applications in fermented feed. This study examined the effects of a 6% dry PP additive on the ensiling characteristics, antioxidant activity, metabolites, bacterial community, and <em>in vitro</em> ruminal fermentation, methane (CH<sub>4</sub>) emission of corn silage ensiled for 7 days and 60 days using microbiome and metabolome analyses. PP-treated silage inhibited (<em>P</em> < 0.05) protein degradation by reducing ammonia nitrogen and non-protein nitrogen concentrations during ensiling. The PP additive increased (<em>P</em> < 0.05) water-soluble carbohydrate content and reduced ethanol production in corn silage. <em>Lactiplantibacillus</em> dominated PP-treated silage at the initial ensiling stage, while <em>Levilactobacillus</em> prevailed at the final stage<em>.</em> Notably, the PP additive exhibited strong antioxidant activity by modulating antioxidant enzymes and flavonoid biosynthesis mediated by key metabolites (epigallocatechin and catechin). Correlation analysis identified <em>Lactiplantibacillus</em>, <em>Citrobacter</em>, <em>Phytobacter</em> and <em>Burkholderia</em> as key microbes in the production of antioxidant metabolites and enzymes in PP-treated silage. Additionally, PP supplementation reduced (<em>P</em> < 0.05) <em>in vitro</em> ruminal CH<sub>4</sub> and nitrogen losses, while decreasing dry matter (DM) digestibility in corn silage. In summary, PP-treated corn silage enhanced antioxidant properties and reduced the nitrogen losses and <em>in vitro</em> ruminal CH<sub>4</sub> emissions, but lowered DM digestibility. Thus, PP can be recommended as a silage additive, though the dry PP level should be lower than that used in this study.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132544"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-sized aerobic granular biofilm: stable biotechnology to improve nitrogen removal and reduce sludge yield 大型好氧颗粒生物膜：稳定的生物技术，提高氮的去除，降低污泥产量

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132543

Haibo Ma , Sihao Chen , Linhuan Lv , Zhou Ye , Jiaqi Yang , Binbin Wang , Jinte Zou , Jun Li , Ramon Ganigué

{"title":"Large-sized aerobic granular biofilm: stable biotechnology to improve nitrogen removal and reduce sludge yield","authors":"Haibo Ma , Sihao Chen , Linhuan Lv , Zhou Ye , Jiaqi Yang , Binbin Wang , Jinte Zou , Jun Li , Ramon Ganigué","doi":"10.1016/j.biortech.2025.132543","DOIUrl":"10.1016/j.biortech.2025.132543","url":null,"abstract":"<div><div>Three parallel sequencing batch reactors (control, small-sized polyurethane sponge (PUS) (3.0 mm), and large-sized PUS (10.0 mm)) were used to investigate aerobic granular biofilm (AGB) characteristics. Results show that 10.0 mm PUS facilitated rapid formation of large-sized AGB (AGB<sub>L</sub>), which exhibited higher biomass concentration (8.5 g/L) and faster settling velocity (69.2–159.3 m/h) than aerobic granular sludge (AGS) (3.2 g/L and 38.6–80.0 m/h). The AGB<sub>L</sub> system also maintained long-term structural stability with a lower instability coefficient (0.004–0.018 min<sup>−1</sup>) than AGS (0.053–0.090 min<sup>−1</sup>). Additionally, during long-term operation, the AGB<sub>L</sub> system achieved excellent removal efficiencies for NH<sub>4</sub><sup>+</sup>-N (99.6 ± 0.4 %) and total nitrogen (92.3 ± 2.6 %), and exhibited a lower sludge yield (0.05 gVSS/gCOD) than AGS (0.14 gVSS/gCOD). The larger size and compact structure of AGB<sub>L</sub> increased anoxic/anaerobic zones, enriching denitrifying and hydrolytic/fermentative bacteria. These findings highlight AGB<sub>L</sub> with large PUS as a more promising biotechnology for practical applications than conventional AGS.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132543"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial-algal symbiotic system drives reconstruction of nitrogen, phosphorus, and methane cycles for purification of pollutants in aquaculture water 微生物-藻类共生系统推动氮、磷和甲烷循环的重建，以净化水产养殖用水中的污染物

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132531

Jun Gao , Qi Mang , Quanjie Li , Yi Sun , Gangchun Xu

{"title":"Microbial-algal symbiotic system drives reconstruction of nitrogen, phosphorus, and methane cycles for purification of pollutants in aquaculture water","authors":"Jun Gao , Qi Mang , Quanjie Li , Yi Sun , Gangchun Xu","doi":"10.1016/j.biortech.2025.132531","DOIUrl":"10.1016/j.biortech.2025.132531","url":null,"abstract":"<div><div>Intensive aquaculture’s excessive nitrogen, phosphorus, and methane emissions caused environmental degradation. This study explored how algae-bacteria symbiotic systems (ABSS) enhanced water purification by regulating element cycles. We established a <em>Chlorella pyrenoidosa</em>-<em>Bacillus subtilis</em> symbiotic system. At a 1:1 bacteria-to-algae ratio, chlorophyll <em>a</em> and cell dry weight were highest. <em>C. pyrenoidosa</em> supplied organic acids, carbohydrates, and amino acids to <em>B. subtilis</em>, which reciprocated with amino acids, purines, and vitamins. ABSS significantly reduced total nitrogen, ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N), nitrite (NO<sub>2</sub><sup>−</sup>-N), nitrate (NO<sub>3</sub><sup>−</sup>-N), phosphate (PO<sub>4</sub><sup>3−</sup>-P), total phosphorous, dissolved organic carbon, and chemical oxygen demand in aquaculture water. It reshaped microbial communities and enriched key genus (<em>Limnohabitans</em>, <em>Planktophila</em>, <em>Polaromonas</em>, <em>Methylocystis</em>) and upregulating genes linked to organic phosphate mineralization, methane oxidation, and nitrate reduction. These changes strengthened nitrogen-phosphorus-methane cycle coupling, boosting water purification. ABSS offers an eco-engineering solution for aquaculture pollution by optimizing microbial interactions and nutrient cycling.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132531"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deuterium stress Reprograms Chlorella sorokiniana Metabolism: Coupling photosynthetic suppression with carbon Reserve Surge 氘胁迫重编程小球藻代谢：光合抑制与碳储备激增的耦合

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132548

Tianfei Li, Yixian He, Shanni Ke, Zexia Cai, Zhuo Jiang

{"title":"Deuterium stress Reprograms Chlorella sorokiniana Metabolism: Coupling photosynthetic suppression with carbon Reserve Surge","authors":"Tianfei Li, Yixian He, Shanni Ke, Zexia Cai, Zhuo Jiang","doi":"10.1016/j.biortech.2025.132548","DOIUrl":"10.1016/j.biortech.2025.132548","url":null,"abstract":"<div><div>Microalgae convert inorganic substrates into stable isotope-labeled compounds, ideal for deuterated compound production. However, the mechanism by which deuterium affects the growth and metabolism of microalgae remains unclear. This study aims to reveal the effects of deuterium on the growth and metabolic processes of <em>Chlorella sorokiniana</em> and to clarify the interaction between them. After deuterium treatment, cell growth and the accumulation of photosynthetic pigments were significantly inhibited, leading to reduced photosynthetic efficiency and blocked energy transfer. Under 100% D<sub>2</sub>O conditions, the accumulation of starch and lipids was enhanced, with starch content reaching up to 52% of dry cell weight and lipid content reaching 22%. Transcriptomics revealed deuterium stress inhibited photosynthesis-related genes while upregulating pathways for starch, fatty acid, and nucleic acid synthesis. These findings reveal the adaptation mechanism of microalgae to deuterium treatment and provide valuable insights for the utilization of microalgae in the production of deuterated organic compounds.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132548"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ cascaded and selective bio-preparation of galactose-derived acids from cheese whey powder by the bifunctional regulation of resting-cell catalysis 通过静息细胞催化的双功能调节，原位级联和选择性地从奶酪乳清粉中制备半乳糖衍生酸

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-14 DOI: 10.1016/j.biortech.2025.132527

Xia Hua , Rui Zhang , Yating Hu , Dylan Liu , Yong Xu

{"title":"In-situ cascaded and selective bio-preparation of galactose-derived acids from cheese whey powder by the bifunctional regulation of resting-cell catalysis","authors":"Xia Hua , Rui Zhang , Yating Hu , Dylan Liu , Yong Xu","doi":"10.1016/j.biortech.2025.132527","DOIUrl":"10.1016/j.biortech.2025.132527","url":null,"abstract":"<div><div>To valorize the excess galactose resources and prepare value-added chemicals for applications in the food and biomedical industries, <em>Gluconobacter oxydans</em> was employed to realize the cascaded and selective bio-preparation of galactose-derived acids. The bifunctional regulatory mechanism of Ca<sup>2+</sup> was the <em>in-situ</em> controllable preparation of calcium galactoate and 2-ketogalactonate by cleverly employing solubility difference. The presence of Ca<sup>2+</sup> could additionally intensify bioconversion efficiency, leading to not only increased bioconversion rate to 1.5 g/L/h, but also improved yield by 18 %. Finally, by combining enzyme hydrolysis, yeast and bacterial fermentation, 100 g cheese whey powder was effectively bio-transformed into 22.2 g bioethanol, 16.8 galactonic acid and 34.4 g 2-ketogalactonic acid, with glucose and galactose utilization rate of 83.4 % and 97.1 %. Overall, this cascading and precise regulation process not only provided a reliable idea for the downstream outlet of galactose, importantly, but also established a potential production technology for 2-ketogalactonic acid.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132527"},"PeriodicalIF":9.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of organic pollutant by natural biofilm based biophotovoltaic cells: The combined role of illumination, reactive oxygen species, and enhanced electron transfer 基于天然生物膜的生物光伏电池降解有机污染物：光照、活性氧和增强电子转移的联合作用

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-13 DOI: 10.1016/j.biortech.2025.132537

Zihan Qi, Xiuyi Hua, Anfeng Li, Haiyang Liu, Deming Dong, Dapeng Liang, Zhiyong Guo, Na Zheng

{"title":"Degradation of organic pollutant by natural biofilm based biophotovoltaic cells: The combined role of illumination, reactive oxygen species, and enhanced electron transfer","authors":"Zihan Qi, Xiuyi Hua, Anfeng Li, Haiyang Liu, Deming Dong, Dapeng Liang, Zhiyong Guo, Na Zheng","doi":"10.1016/j.biortech.2025.132537","DOIUrl":"10.1016/j.biortech.2025.132537","url":null,"abstract":"<div><div>Autotrophic biofilms in natural water can produce reactive oxygen species (ROS) and degrade organic pollutants. To test the feasibility of applying natural biofilms in biophotovoltaic (BPV) cells, a simple single-chamber BPV using biofilms developed in lake water as the microbial anode was constructed, and electricity production and pollutant removal capacity of the BPV were evaluated. The natural biofilm BPV (NB-BPV) established was a robust and self-sustaining BPV with positive light response in electricity generation and sustainable power generation in the dark. Under visible light illumination, the voltage of the NB-BPV reached a maximum output of 150.6 ± 0.7 mV, with a power density of 326.4 μW/m<sup>2</sup> (per electrode surface area). Meanwhile, it could effectively degrade sodium dodecylbenzene sulfonate (DBS), while generating electricity, and the removal rate of DBS and TOC in 36 h was 77.1 % and 53.2 %, respectively. Under sunlight, NB-BPV could also produce electricity steadily in lake and the removal rate of DBS in simulated lake water was 93.7 % (120 h). Visible light significantly affected the performance of NB-BPV mainly through photosynthesis. Photosynthesis of biofilm promoted electricity generation and significantly enhanced the degradation of DBS by promoting electron transfer activity and generating ROS. Compared with biofilm system, the closed-circuit in NB-BPV promoted electron transfer, allowing more efficient degradation of DBS at relatively low level of ROS. Such a novel self-sustainable BPV has the potential to degrade refractory pollutants, and to be used in natural water.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132537"},"PeriodicalIF":9.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards robust partial nitrification in low-ammonia wastewater: Electrospinning nanofiber composite-enhanced hydrogel beads immobilized comammox Nitrospira 低氨废水的强力部分硝化作用：静电纺丝纳米纤维复合材料增强水凝胶珠固定化comammox硝化螺旋菌

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-13 DOI: 10.1016/j.biortech.2025.132541

Peng Tang , Liangang Hou , Meiling Yin , Feng Huang , Zhengwei Pan , Tianhao Shi , Jun Li , Yuhan Zhu , Xin Zhang , Peng Gao

{"title":"Towards robust partial nitrification in low-ammonia wastewater: Electrospinning nanofiber composite-enhanced hydrogel beads immobilized comammox Nitrospira","authors":"Peng Tang , Liangang Hou , Meiling Yin , Feng Huang , Zhengwei Pan , Tianhao Shi , Jun Li , Yuhan Zhu , Xin Zhang , Peng Gao","doi":"10.1016/j.biortech.2025.132541","DOIUrl":"10.1016/j.biortech.2025.132541","url":null,"abstract":"<div><div>Stable partial nitrification (PN) in low-ammonia wastewater has been a critical challenge, but comammox <em>Nitrospira</em> shows great potential in PN systems due to unique physiological characteristics. Polyvinyl alcohol/sodium alginate electrospinning nanofiber (PVA/SA-EN) and polyvinylidene fluoride EN (PVDF-EN) were used to construct EN hydrogel beads (ENHB) with sandwich and core–shell structures, respectively. Comammox HB (cHB) and three types of comammox ENHB (cENHB) were assembled by immobilizing comammox sludge and operated for 60 days in low-ammonia wastewater. Results showed a significant correlation between PN performance, HB pore structure, and comammox <em>Nitrospira</em> abundance. With superior pore structure and mechanical strength, PVA/SA-PVDF-cENHB achieved a nitrite accumulation rate of 55.08 %, indicating enhanced PN performance. The contribution of comammox <em>Nitrospira</em> to PN was 73.19 %. Its abundance in PVA/SA-PVDF-cENHB was 5.56 × 10⁶ copies/(g sludge), 1.16–1.95-fold higher than the other three HB. Nanofiber composite-enhanced hydrogel immobilizes comammox <em>Nitrospira</em> provides new ideas for achieving robust PN in low-ammonia wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132541"},"PeriodicalIF":9.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in multi-omics technologies for identifying metabolic engineering targets and improving lipid production in microalgae 多组学技术在确定代谢工程目标和提高微藻类脂质产量方面的进展

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-12 DOI: 10.1016/j.biortech.2025.132501

Chun-xiao Yan , Shuai Zhang , Lu-wei Xu , Han Gao , Zi-xu Zhang , Wang Ma , Xiao-man Sun

{"title":"Advances in multi-omics technologies for identifying metabolic engineering targets and improving lipid production in microalgae","authors":"Chun-xiao Yan , Shuai Zhang , Lu-wei Xu , Han Gao , Zi-xu Zhang , Wang Ma , Xiao-man Sun","doi":"10.1016/j.biortech.2025.132501","DOIUrl":"10.1016/j.biortech.2025.132501","url":null,"abstract":"<div><div>Polyunsaturated fatty acids (PUFAs), such as γ-linolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid, are highly valued in the global market due to their physiological effects and health benefits. Concerns related to overfishing and marine ecosystem degradation have driven interest in microalgal lipids as a sustainable and eco-friendly alternative for PUFA production. Despite some success in commercializing microalgal lipid products, they still fail to meet global demand. Advances in high-throughput omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, have deepened the understanding of lipid biosynthesis in microalgae. This review explores the potential of multi-omics approaches to elucidate PUFA biosynthesis pathways, identify key regulatory genes, and optimize metabolic engineering strategies for enhanced lipid production. Additionally, this review discusses how multi-omics technologies address challenges in large-scale cultivation, promoting the industrialization of microalgal lipid productions. These insights provide a foundation for improving microalgal PUFA yields to meet growing global demand.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"429 ","pages":"Article 132501"},"PeriodicalIF":9.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0