Bioresource Technology最新文献_第8页

Efficient production of ectoine from Jerusalem artichoke using engineered Escherichia coli 利用工程大肠杆菌从菊芋中高效生产异托因

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-25 DOI: 10.1016/j.biortech.2025.132589

Hao Zhang , Hairui Tong , Qiang Yin , Yibin Qiu , Hong Xu , Sha Li

引用次数: 0

Efficient nitrogen removal by the aerobic denitrifying bacterium Pseudomonas stutzeri RAS-L11 under triple stresses of high alkalinity, high salinity, and tetracycline: From performance to mechanism 好氧反硝化细菌斯图氏假单胞菌RAS-L11在高碱度、高盐度和四环素三重胁迫下的高效脱氮：从性能到机理

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-25 DOI: 10.1016/j.biortech.2025.132590

Wanying Li, Rui Huo, Yiling Di, Chun Liu, Shilei Zhou

{"title":"Efficient nitrogen removal by the aerobic denitrifying bacterium Pseudomonas stutzeri RAS-L11 under triple stresses of high alkalinity, high salinity, and tetracycline: From performance to mechanism","authors":"Wanying Li, Rui Huo, Yiling Di, Chun Liu, Shilei Zhou","doi":"10.1016/j.biortech.2025.132590","DOIUrl":"10.1016/j.biortech.2025.132590","url":null,"abstract":"<div><div>Efficient aerobic denitrification bacteria are rarely reported under triple stresses of high alkalinity, high salinity, and tetracycline. Here, strain <em>Pseudomonas stutzer</em>i RAS-<em>L</em>11 was isolated, under the optimal reaction conditions of C/N = 6, sodium acetate as carbon source, and pH 7.0–11.0. Moreover, RAS-<em>L</em>11 showed perfect nitrogen removal performance under dual and triple stresses. Specifically, the mean removal efficiency of total dissolved nitrogen for different medium (nitrate, nitrite, ammonia, nitrate and ammonia, and nitrite and ammonia) reached 92.35 %, 66.85 %, 71.33 %, 89.42 %, and 68.76 % under triple stresses. Nitrogen balance results indicated that biomass nitrogen accounted for a small percentage (4.48 % to 20.79 %). Furthermore, the nitrogen metabolism pathways and tetracycline, salinity, and alkaline tolerance-associated genes were also confirmed. Strain RAS-<em>L</em>11 achieved 42.67–70.72 % NO<sub>3</sub><sup>−</sup>-N and 83.72–88.53 % NH<sub>4</sub><sup>+</sup>-N removal efficiencies in both sterilized and actual systems treating pharmaceutical wastewater. Our characterization of the RAS-<em>L</em>11 provides a reference for nitrogen removal of pharmaceutical wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132590"},"PeriodicalIF":9.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant 大肠杆菌系统代谢工程高水平生产脱氧紫素，一种天然着色剂

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-24 DOI: 10.1016/j.biortech.2025.132584

Mi Tang , Zhenhui Pan , Minghui Jin , Hengwei Zhang , Xuewei Pan , Zhiming Rao

{"title":"Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant","authors":"Mi Tang , Zhenhui Pan , Minghui Jin , Hengwei Zhang , Xuewei Pan , Zhiming Rao","doi":"10.1016/j.biortech.2025.132584","DOIUrl":"10.1016/j.biortech.2025.132584","url":null,"abstract":"<div><div>Deoxyviolacein is a natural colorant with various biological properties, widely applied in cosmetic and pharmaceutical fields. However, current methods of deoxyviolacein production by natural producers may cause highly lethal infections in humans, limiting the sustainable production of deoxyviolacein. Here, an <span>l</span>-tryptophan-producing <em>Escherichia coli</em> strain was engineered for efficient deoxyviolacein production. First, the deoxyviolacein synthesis pathway was introduced and optimized to construct a base strain. Second, multi-modular engineering was conducted for further optimization, including engineering of the glucose uptake system and central metabolism and enhancement of precursor supply. To coordinate metabolic flux distribution, the optimal expression of <em>aroG</em><sup>Q151F</sup>, <em>ppsA</em> and <em>tktA</em> was tuned by generating libraries of tunable intergenic regions coupled with a novel <span>l</span>-tryptophan biosensor. Finally, the best-performing strain successfully accumulated 12.18 g/L of deoxyviolacein from glucose, showing a competitive deoxyviolacein titer reported to date and providing a paradigm for the production of value-added aromatic compounds in <em>E. coli</em>.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132584"},"PeriodicalIF":9.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-driven insights for enhanced cellulose conversion to 5-hydroxymethylfurfural using machine learning 使用机器学习增强纤维素转化为5-羟甲基糠醛的数据驱动见解

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-23 DOI: 10.1016/j.biortech.2025.132582

Yanming Qiao , Ehsan Kargaran , Hao Ji , Meysam Madadi , Saeed Rafieyan , Dan Liu

{"title":"Data-driven insights for enhanced cellulose conversion to 5-hydroxymethylfurfural using machine learning","authors":"Yanming Qiao , Ehsan Kargaran , Hao Ji , Meysam Madadi , Saeed Rafieyan , Dan Liu","doi":"10.1016/j.biortech.2025.132582","DOIUrl":"10.1016/j.biortech.2025.132582","url":null,"abstract":"<div><div>Converting cellulose into 5-Hydroxymethylfurfural (HMF) provides a promising strategy for creating bio-based chemicals, offering sustainable alternatives to petroleum-based materials in polymers, biofuels, and pharmaceuticals. However, the efficient production of HMF from cellulose is challenged by the complex interplay of numerous operational variables. This study develops a machine learning (ML) model to optimize HMF production and conducts a feature importance analysis to identify the key factors affecting HMF yield. Additionally, a Bayesian optimization is employed for multi-objective optimization aimed at maximizing HMF yield. A comprehensive dataset, sourced from existing literature, was subjected to statistical analysis to elucidate the influence of each factor on HMF production. Among the eight models evaluated, the CatBoost Regressor emerged as the most effective, delivering robust predictive performance with R<sup>2</sup> of 0.76 during testing and exhibiting low RMSE (4.72) and MAE (5.2) values. Feature importance analysis revealed that operational conditions, particularly time and temperature, were the most significant, accounting for 41.0% of the variability, followed by catalyst properties at 33.0% and solvent properties at 26.0%. The ML-based optimization achieved an HMF yield of 48.1%, with relative errors of −1% and 2.5% in the first (47.6%) and second (49.3%) runs of experimental validation, respectively. This research showcases ML’s ability to address challenges in cellulose-to-HMF conversion, offering insights for optimizing production and advancing sustainable manufacturing.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132582"},"PeriodicalIF":9.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of mannitol as a potential substrate for production of 2′-fucosyllactose in Yarrowia lipolytica 甘露醇作为溶脂耶氏菌生产2′-焦酰基乳糖的潜在底物的研究

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-23 DOI: 10.1016/j.biortech.2025.132583

Yan Zhang, Haiyan Liu, Mengmeng Liu, Qingsheng Qi

引用次数: 0

Optimization of synergistic microwave and zero–valent iron co–pretreatment for anaerobic digestion of waste activated sludge 微波与零价铁协同预处理废活性污泥厌氧消化工艺优化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-22 DOI: 10.1016/j.biortech.2025.132568

Seung–han Ha , Seung Gu Shin , Johng–Hwa Ahn

{"title":"Optimization of synergistic microwave and zero–valent iron co–pretreatment for anaerobic digestion of waste activated sludge","authors":"Seung–han Ha , Seung Gu Shin , Johng–Hwa Ahn","doi":"10.1016/j.biortech.2025.132568","DOIUrl":"10.1016/j.biortech.2025.132568","url":null,"abstract":"<div><div>This study optimized co<strong>-</strong>pretreatment of microwave temperature (<em>T</em><sub>MW</sub>) and zero-valent iron dosage ([ZVI]) to enhance anaerobic digestion (AD) of waste activated sludge (WAS). WAS was pretreated at <em>T</em><sub>MW</sub> = 100, 150, or 200 °C and [ZVI] = 1, 3, or 5 g/L using a central composite design. Optimal co-pretreatment (<em>T</em><sub>MW</sub> = 168 °C and [ZVI] = 5 g/L) reduced the ratio of volatile solids (VS) to total solids by 21.5 %, increased the solubilization ratio seven–fold, removed 53.5 % of phosphate compared to WAS partly because of lignin fragmentation. Biochemical methane potential identified optimal conditions (<em>T</em><sub>MW</sub> = 164 °C and [ZVI] = 4.8 g/L), enhancing VS removal by 70.9 %, methane yield by 60 %, and reducing hydrogen sulfide by 82.4 % compared to Control. Kinetic analysis indicated 61 – 108 % increase in maximum methane production rate. Microbial analysis revealed increased acetoclastic methanogens and decreased hydrogenotrophic methanogens. Thus, microwave–ZVI co-pretreatment enhanced WAS biodegradability and AD efficiency.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132568"},"PeriodicalIF":9.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Nitrous oxide emissions treating hypersaline wastewater in suspended and attached partial nitritation – anammox reactors” [Bioresour. Technol. 424 (2025) 132266] “在悬浮和附著部分硝化-厌氧氨氧化反应器中处理高盐废水的氧化亚氮排放”的勘误[生物资源]。技术号424 (2025)132266]

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132557

Lin Gao, Sheldon Tarre, Michal Green

引用次数: 0

Iron-carbon enhanced constructed wetland microbial fuel cells for tetracycline wastewater treatment: Efficacy, power generation, and the role of iron-carbon 铁碳强化人工湿地微生物燃料电池处理四环素废水：效能、发电和铁碳的作用

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132578

Zhiyuan Du , Sai Bai , Jin Qian , Peng Zhan , Fengping Hu , Xiaoming Peng

{"title":"Iron-carbon enhanced constructed wetland microbial fuel cells for tetracycline wastewater treatment: Efficacy, power generation, and the role of iron-carbon","authors":"Zhiyuan Du , Sai Bai , Jin Qian , Peng Zhan , Fengping Hu , Xiaoming Peng","doi":"10.1016/j.biortech.2025.132578","DOIUrl":"10.1016/j.biortech.2025.132578","url":null,"abstract":"<div><div>Tetracycline (TC) antibiotics wastewater is a serious threat to human health and environment. In this study, four groups of laboratory-scale constructed wetlands (CWs) with different configurations were constructed to evaluate the removal efficiency of iron-carbon (Ic) coupled constructed wetland microbial fuel cells (CW-MFC) system for different pollutants removal and bioelectricity production. The results showed that the addition of Ic significantly promoted the removal of contaminants. The maximum removal rates of COD, TN, NH<sub>4</sub><sup>+</sup>-N, and TP were 86.13 %, 81.60 %, 79.07 %, and 97.35 %, respectively. In particular, the removal rates of TC reached 100 %. 3D-EEM analysis further confirmed the role of Ic in promoting organic degradation. The Ic-CW-MFC system also showed superiority in power generation performance with peak power density of 7.90 mW/m<sup>2</sup> (internal resistance is 10 Ω), 88.07 % higher than the traditional CW-MFC, while the internal resistance was 68.21 % lower. Therefore, when Ic is used as the substrate of CW-MFC system, its decontamination and electricity generation performance is the best. Analysis of RDA was used to elucidate the relationship of four CWs, dominant strains and environmental factors (pH, ORP and DO). The performance of traditional CWs decreased significantly after TC addition (5–20 mg/L), but Ic-CW-MFC could effectively alleviate the inhibition effect caused by high-concentration TC wastewater. The working mechanism of Ic-CW-MFC in TC wastewater was further analyzed through typical cycle experiment and characterization. The results showed that Ic-CW-MFC is an efficient and economical wastewater treatment technology, which has great potential application value in the treatment of wastewater containing TC.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132578"},"PeriodicalIF":9.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and characterization of acid-tolerant Chlorophyta division microalgae for potential metabolites production in a bubble column bioreactor: A ready-to-industrial scale screening 气泡柱生物反应器中用于潜在代谢物生产的耐酸绿藻分裂微藻的选择和特性：准备工业规模的筛选

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132579

Luigi Marra , Elena Aurino , Francesca Raganati , Antonino Pollio , Antonio Marzocchella

{"title":"Selection and characterization of acid-tolerant Chlorophyta division microalgae for potential metabolites production in a bubble column bioreactor: A ready-to-industrial scale screening","authors":"Luigi Marra , Elena Aurino , Francesca Raganati , Antonino Pollio , Antonio Marzocchella","doi":"10.1016/j.biortech.2025.132579","DOIUrl":"10.1016/j.biortech.2025.132579","url":null,"abstract":"<div><div>Microalgae are promising candidates for sustainable bioprocesses owing to their ability to capture/utilize CO<sub>2</sub> and produce valuable metabolites. However, strain-to-strain variability poses a challenge to industrial scalability. This paper reports a study on the screening of 28 Chlorophyta microalgal strains from six species cultivated under standardized semi-continuous conditions in a bubble column bioreactor. Growth kinetics, nitrogen uptake, and metabolite accumulation were analyzed to identify strains characterized by promising biomass/metabolite productivities.</div><div>The results indicated two distinct kinetic behaviors: a large fraction of the investigated strains was characterized by a common biomass/nitrate uptake behavior, and a subset of the strain pool was characterized by atypical nitrogen assimilation patterns. Strains characterized by high biomass growth rates are characterized by high lipid productivity. Strains with intermediate biomass growth rates are characterized by high carbohydrate accumulation. <em>Chlorella vulgaris</em> (ACUF 058) and <em>Chloroidium saccharophilum</em> (ACUF 050) emerged as strong candidates for carbohydrate and protein production, respectively.</div><div>This study proposed a systematic procedure for strain selection, bridging laboratory screening, and industrial feasibility. These results emphasize the role of tailored cultivation strategies in optimizing biomass yield and metabolite productivity. Further research integrating genomic and proteomic insights will enhance strain-specific process optimization, facilitating the large-scale deployment of microalgal bioprocesses.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132579"},"PeriodicalIF":9.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced expression of glycolysis regulators KmGcr1p and KmGcr2p in Kluyveromyces marxianus: An efficient strategy for high-temperature ethanol production from inulin 马氏克鲁维菌糖酵解调节因子KmGcr1p和KmGcr2p的表达增强：菊粉高温乙醇生产的有效策略

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132559

Lan Xu , Hui-Min Wang , Feng-Yi Li , Wen-Hui Zhao , Dan Yue , Zhen-Ming Chi , Guang-Lei Liu

{"title":"Enhanced expression of glycolysis regulators KmGcr1p and KmGcr2p in Kluyveromyces marxianus: An efficient strategy for high-temperature ethanol production from inulin","authors":"Lan Xu , Hui-Min Wang , Feng-Yi Li , Wen-Hui Zhao , Dan Yue , Zhen-Ming Chi , Guang-Lei Liu","doi":"10.1016/j.biortech.2025.132559","DOIUrl":"10.1016/j.biortech.2025.132559","url":null,"abstract":"<div><div>Bioethanol production from non-food biomass has emerged as a sustainable alternative to satisfy future energy demands. <em>Kluyveromyces marxianus</em> stands out as a potential consolidated bioprocessing (CBP) strain for high-temperature ethanol production from inulin. Nevertheless, enhancing its fermentation efficiency remains crucial. Here, we characterized its glycolysis regulator KmGcr2p and demonstrated that KmGcr2p boosts the expression of target genes controlled by another glycolysis regulator, KmGcr1p, through their interaction. Furthermore, we engineered the strain Ogcr1 + 2 through co-overexpressing <em>KmGCR1</em> and <em>KmGCR2</em>, leading to a 55.8 % increase in inulinase activity, improved growth at 37 °C to 45 °C and under 10 % ethanol, and enhanced ethanol production at 42 °C by 36.0 % from inulin and 39.5 % from Jerusalem artichoke tuber flour. Our results highlight that co-overexpression of glycolysis regulators KmGcr1p and KmGcr2p can simultaneously enhance inulin saccharification, thermotolerance, ethanol tolerance, and ethanol production, presenting a promising and feasible strategy for high-temperature CBP ethanol production from inulin and inulin-rich feedstocks.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132559"},"PeriodicalIF":9.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0