Bioresource Technology最新文献

{"title":"Engineering of β-glucosidase CaBGL with improved performance in cellulose hydrolysis.","authors":"Cai You, Xin Zheng, Kuan Qi, Sheng Dong, Ya-Jun Liu, Chao Chen, Qiu Cui, Yingang Feng","doi":"10.1016/j.biortech.2025.133424","DOIUrl":"10.1016/j.biortech.2025.133424","url":null,"abstract":"<p><p>β-Glucosidase (BGL) plays a crucial role in lignocellulose utilization by alleviating cellobiose inhibition of cellulases. Incorporation of the BGL from Caldicellulosiruptor sp. F32 (CaBGL) enhanced the overall efficiency of the consolidated bio-saccharification process. To optimize BGL performance under industrial conditions, we established a thermostable green fluorescent protein-based high-throughput screening platform coupled with structure-informed semi-rational design, enabling the generation of functionally enhanced CaBGL mutants. This approach identified mutant M418T, which exhibited more than two-fold catalytic activity compared to that of wild type in both the absence and presence of glucose at various concentrations. In vitro cellulose saccharification showed that M418T increased the saccharification rate coefficient by 43.27 % compared to the wild-type. The mechanisms underlying its improved property were further elucidated through structural analysis and molecular docking. Consequently, this work presents an effective approach for enhancing the performance of CaBGL and demonstrates the potential of a promising catalyst in lignocellulose conversion.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133424"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and performance of ultraviolet tolerant bacterium strain for pretreatment and quorum quenching to control reverse osmosis biofouling","authors":"Xiaolei Zhang ,&nbsp;Jingrui Liu ,&nbsp;Shanna Shu ,&nbsp;Yingying Qi ,&nbsp;Huhui Chen ,&nbsp;Qiang Liu ,&nbsp;Ping Yao ,&nbsp;Long D. Nghiem","doi":"10.1016/j.biortech.2025.133422","DOIUrl":"10.1016/j.biortech.2025.133422","url":null,"abstract":"<div><div>Conventional biocides and UV disinfection are often ineffective against biofouling in reverse osmosis (RO). This study explores a new approach to combine <em>Staphylococcus</em> sp. SSN, a UV-tolerant quorum quenching (QQ) bacterium isolated from activated sludge and UV treatment to defer the onset of biofouling. Under UV exposure, immobilized <em>Staphylococcus</em> sp. SSN retained QQ activity, reducing biofilm formation (by 81 %), and extracellular polymeric substance production (by 22 %). Within 2 h, the combined UV-QQ treatment achieved 3.5-log bacterial inactivation, outperforming UV alone. The observed enhanced effect is likely due to suppressed quorum sensing and biofilm gene expression, reducing UV shielding and increasing oxidative stress. In real wastewater experiments, UV-QQ pretreatment lowered RO flux decline to 45 % after 6 days, compared to 70 % in the control. These findings demonstrate the potential of integrating QQ bacteria with UV disinfection to slow down biofouling development in RO for water reuse applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133422"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-adaptive evolution of glutamate decarboxylase enables gamma-aminobutyric acid biosynthesis without pH control.","authors":"Chenshuo Song, Jie Luo, Jun Qiao, Zhongmei Liu, Zhongyi Cheng, Zhemin Zhou, Laichuang Han","doi":"10.1016/j.biortech.2025.133432","DOIUrl":"10.1016/j.biortech.2025.133432","url":null,"abstract":"<p><p>The strict pH-dependent catalytic activity of glutamate decarboxylase (GAD) necessitates substantial acid consumption during γ-aminobutyric acid (GABA) biosynthesis, increasing costs and environmental impact. To overcome this limitation, we engineered glutamate decarboxylase from Escherichia coli (EcGadB) for enhanced activity at neutral pH. Using constant pH molecular dynamics (CpHMD) simulations, we targeted the pH-sensitive γ-carboxyl-binding loop (γ-CBL). Through three rounds of Adaptive Iterative Evolution (AIE) guided by a GABA biosensor, mutant M3 (Y51L/A56P/D68N/D69T) was obtained, which exhibited a 43.5-fold enhancement in catalytic efficiency (k_cat/K_m) at pH 7.5. Gaussian-accelerated MD simulations indicated that M3 stabilizes catalytic conformations of γ-CBL, decoupling its activity from acidic conditions. By further design, mutant M4 (M3-Q348D/M431K) with significantly improved thermostability was obtained. M4 was applied to three pH-control-free catalytic systems: achieving 360 g/L GABA in enzymatic catalysis (50 °C, 8 h), 219 g/L in whole-cell biocatalysis, and 60 g/L (1.71 g/L/h space-time yield) in high-cell-density fermentation. This study establishes an integrated computational/directed evolution loop engineering paradigm, enabling efficient, sustainable, and economically viable enzymatic GABA production by eliminating pH control requirements and enhancing process robustness.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133432"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-stage anaerobic digestion of fruit and vegetable waste: optimization of dark fermentation through thermal pretreatment and co-digestion with sugar-rich wastewater.","authors":"Gaia Mazzanti, Francesca Demichelis, Debora Fino, Tonia Tommasi","doi":"10.1016/j.biortech.2025.133423","DOIUrl":"10.1016/j.biortech.2025.133423","url":null,"abstract":"<p><p>Fruit and vegetable waste (FVW) poses environmental challenges but can be valorized via dark fermentation (DF) and two-stage anaerobic digestion (TSAD) to produce H₂ and CH₄. This study evaluated the effects of culture medium and thermal pretreatment of inoculum in DF, testing various sugars and jam wastewater (JWW) as a renewable sugar source. Thermal pretreatment at 60 °C for 30 min enriched H₂-producers, while culture medium had no significant effect. Glucose and sucrose allow H₂ yield of 6.5 and 45.8 NmL/g_VS, respectively. JWW proved suitable for DF of both raw and pretreated FVW, achieving H₂ yields of 67.1 and 99.5NmL/g_VS, respectively. Volatile fatty acids after DF indicated active fermentation pathways reaching 7.2 g/L in JWW_FVW_1:1. In the TSAD, CH₄ production reached 184NmL/g_VS, surpassing conventional single-stage digestion. These results demonstrate the potential of TSAD systems using FVW and JWW for efficient simultaneous H₂ and CH₄ production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133423"},"PeriodicalIF":9.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of simulated dairy wastewater using artificial amoeba- based enzyme carriers for enhanced degradation of complex organic compounds","authors":"Xunan Zhang ,&nbsp;Wenjie Liu ,&nbsp;Wei Zong","doi":"10.1016/j.biortech.2025.133425","DOIUrl":"10.1016/j.biortech.2025.133425","url":null,"abstract":"<div><div>Conventional methods for treating food industry wastewater are often constrained by low resource recovery and high operational costs. This study introduces an innovative artificial amoeba (AA)-based enzyme carrier designed to enhance the efficiency of dairy wastewater treatment by improving enzyme stability and activity, while simultaneously promoting the resource utilization potential of microalgae. The AA was fabricated via electroformation, encapsulating an agarose-glycerol gel and protease within liposomes to mimic the protective mechanism of natural amoebae. Enzymatic conditions were optimized to evaluate the hydrolytic efficiency on complex organic substrates. Notably, the enzymatic pretreatment of dairy wastewater markedly stimulated the growth of <em>Chlorella vulgaris</em>, achieving a biomass concentration of 1.36 g/L approximately ninefold higher than that obtained with untreated wastewater. This study not only presents a novel strategy for the effective treatment of dairy effluents but also offers a promising pathway for sustainable resource valorization through microalgal biotechnology for the production of high-value bio-based products.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133425"},"PeriodicalIF":9.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of tetracycline resistance genes in silage through targeted lactic acid bacteria inoculation","authors":"Zhang Qing ,&nbsp;Huang Haibei ,&nbsp;Yin Hanxue ,&nbsp;Sun Juan","doi":"10.1016/j.biortech.2025.133416","DOIUrl":"10.1016/j.biortech.2025.133416","url":null,"abstract":"<div><div>The dissemination of antibiotic resistance genes (ARGs) in silage ecosystems poses a critical challenge to ecological stability and public health security. This investigation focuses on tetracycline resistance genes (TRGs), the most prevalent subtype of ARGs in silage, employing a targeted selection strategy for lactic acid bacteria (LAB) inoculants. From 226 isolated LAB strains, four candidates (LP1-3: <em>Lactiplantibacillus plantarum</em>; LC1: <em>Lacticaseibacillus paracasei</em>) demonstrating superior growth kinetics (OD₆₀₀ &gt; 1.6 within 24 h) and rapid acidification capacity (pH &lt; 3.9 within 24 h) were selected. Strains LP3 and LC1 exhibited minimal intrinsic TRGs content. These four strains reduced (p &lt; 0.001) pH, ammonia-N concentration, and coliform bacterial counts of stylo silage. Metagenomic analysis revealed that strains LP1-3 promoted <em>Lactiplantibacillus</em> dominance (0.709–0.975 vs. 0.379–0.509 in the control), while LC1 enhanced <em>Lacticaseibacillus</em> abundance (0.449–0.612 vs. 0.002–0.013 in the control). Ensiling process downregulated 367 and upregulated 227 ARGs. Inoculation with the four LAB strains further enhanced the suppression of ARGs. Among the top 30 TRGs, 22 were reduced by strains LP1-3 and 20 by LC1. Quantitative PCR results showed that strains LP1-3 decreased (p &lt; 0.05) the contents of <em>tetA</em> and <em>tetG</em> during 30 days fermentation. Among the TRGs increased, <em>tetA(60)</em>, <em>tetB(58)</em>, <em>tet(T)</em> were positively correlated with <em>Lactiplantibacillus</em> spp., <em>tetA(58)</em>, <em>tetB(60)</em>, <em>tetA(46)</em>, <em>tetB(46)</em>, <em>tet(43)</em> were significantly correlated with <em>Lacticaseibacillus</em> spp. (R &gt; 0.4, p &lt; 0.001). In conclusion, the fermentation process induced substantial ARGs profile modifications, LAB-mediated microbiome engineering enables TRGs suppression, providing a scientific foundation for precision silage management strategies targeting antimicrobial resistance mitigation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133416"},"PeriodicalIF":9.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High production of glycolic acid by Klebsiella pneumoniae: Engineering metabolic pathways and optimizing culture conditions.","authors":"Sang-Gyu Jeon, Sun-Yeon Heo, Jung-Hyun Ju, Soo-Jin Yeom, Baek-Rock Oh","doi":"10.1016/j.biortech.2025.133418","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133418","url":null,"abstract":"<p><p>Growing concerns regarding environmental pollution caused by non-biodegradable plastic waste have intensified the demand for sustainable biodegradable bioplastics. Glycolic acid (GA), the monomer of polyglycolic acid (PGA), is a key precursor in the production of biodegradable plastics. In this study, a systematic metabolic engineering strategy was employed to enhance GA production in Klebsiella pneumoniae via the xylose oxidation pathway. As a first step, wild-type K. pneumoniae CU (a non-GA-producing strain) was transformed with the pETM-XFEA plasmid harboring four genes involved in the xylose oxidation pathway, resulting in KPGA0, which produced 4.0 ± 0.3 g/L of GA. To further increase metabolic flux, the xylA gene was deleted in KPGA0, yielding the KPGA1 strain, which produced 5.3 ± 0.4 g/L of GA-a 1.32-fold increase. Due to the accumulation of acetic acid and ethylene glycol as major byproducts in KPGA1, genes responsible for their production were subsequently deleted to construct KPGA11. Under optimized culture conditions (pH, agitation speed, and aeration), KPGA11 achieved 15.8 ± 0.6 g/L of GA, a 2.98-fold increase compared to KPGA1. Furthermore, fed-batch cultivation of KPGA11 resulted in a GA titer of 70.1 ± 3.1 g/L, a productivity of 0.730 ± 0.033 g/L/h, and a conversion yield of 0.283 ± 0.003 g/g. To the best of our knowledge, this study is the first to demonstrate high-titer GA production through upregulation of the xylose oxidation pathway in K. pneumoniae, and also reports the highest GA production achieved to date in a microbial system utilizing a carbon source.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133418"},"PeriodicalIF":9.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance study of microbial fuel cell stack system for treating fine chemical wastewater","authors":"Hongwei Chen ,&nbsp;Ao Lei ,&nbsp;Yangfan Song ,&nbsp;Yanmin Li ,&nbsp;Qianyun Wu ,&nbsp;Liuping He ,&nbsp;Chao Han ,&nbsp;Pei Wu ,&nbsp;Xin Yang","doi":"10.1016/j.biortech.2025.133419","DOIUrl":"10.1016/j.biortech.2025.133419","url":null,"abstract":"<div><div>This study developed a stacked anaerobic fluidized bed microbial fuel cell (SAFB-MFC) system for treating refractory fine chemical wastewater containing high concentrations of benzene compounds, while simultaneously generating electricity. The system integrated 45 air–cathode MFC units within a shared anode chamber using a three-dimensional layout, achieving high integration density. Experimental results demonstrated that the total power output increased linearly with the number of connected units, peaking when all are connected. Units in the middle and upper sections exhibited superior performance to those at the bottom, whereas horizontal performance was uniform. Increasing superficial velocity enhanced mass transfer, raising the maximum power density by 18.3 ± 2.1 %. Parallel operation increased the chemical oxygen demand (COD) removal rate by 9.1 % and total power output by 43.5 times compared to a single unit. The system demonstrates significant potential for scalable and efficient application in real industrial wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133419"},"PeriodicalIF":9.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When biomass meet microplastic during dyeing sludge incineration: The impacts on thermal characteristics, gas evolution, and chlorine cycle","authors":"Teng Wang ,&nbsp;Bo Liu ,&nbsp;Qiao Xiong ,&nbsp;Changxin Lu ,&nbsp;Junbin Lian ,&nbsp;Yinuo Pan ,&nbsp;Jilin Bai ,&nbsp;Si Chen ,&nbsp;Yongjie Xue ,&nbsp;Haobo Hou","doi":"10.1016/j.biortech.2025.133410","DOIUrl":"10.1016/j.biortech.2025.133410","url":null,"abstract":"<div><div>Co-combustion of dyeing sludge (DS) and biomass enables energy recovery and conventional pollutant passivation, yet biomass’s impact on microplastics (MPs) degradation remains uncharacterized. This study investigates three representative biomass fuels—crab shell (CS; Ca-rich), pitaya peel (PP; K-rich), and rice husk (RH; Si-rich)—on polyvinyl chloride (PVC) MPs combustion via integrated analysis of combustion characteristics, gas evolution, kinetics, and residue chemistry / mineral analysis. Biomass additives shifted PVC mass loss to lower temperatures and reduced ignition (<em>T_i</em>) and burnout (<em>T_f</em>) temperatures, indicating catalytic degradation by inorganic constituents from biomass. Contrary to expectations, co-combustion of PVC with biomass reduced CO₂, H₂O, and HCl yields. CS achieved 95.70 % HCl suppression (0.35PVC/CS) versus pure PVC. PVC-CS interactions accelerated ignition while suppressing gas emissions, particularly HCl. Co-combustion kinetics remained PVC-dominated, with biomass increasing activation energy (<em>E</em>) for PVC dehydrochlorination (inhibiting HCl release) while decreasing <em>E</em> for devolatilization / char combustion (catalyzing degradation). Among biomass additives, CS demonstrated superior chlorine fixation capacity (443.44 mg/g) through formation of chlorine-bearing minerals (hydroxylapatite, hydrophilite, sylvite) via Ca/K-HCl reactions, with calcium providing the predominant contribution. Collectively, co-combustion of DS with calcium-rich biomass represents a promising waste management strategy for simultaneous MPs degradation and chlorine emission mitigation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133410"},"PeriodicalIF":9.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathway optimization and membrane engineering for highly efficient production of indigoidine in engineered Escherichia coli.","authors":"Zeyu Li, Rui Lu, Heng Hu, Roulin Chen, Dingjie Zhou, Yingying Zhu, Wanmeng Mu","doi":"10.1016/j.biortech.2025.133415","DOIUrl":"10.1016/j.biortech.2025.133415","url":null,"abstract":"<p><p>Recently, indigoidine has garnered increasing attention and demonstrates significant commercial potential, particularly within the textile industry. As a diphenyl quinone compound, indigoidine shows promise as an alternative colorant due to its superior chemical functionality and stability. Consequently, we engineered Escherichia coli BL21(DE3) containing plasmids to efficiently produce indigoidine using glycerol as the sole carbon source. Initially, eighteen combinations of indigoidine synthases and 4'-phosphopantetheinyl transferases (PPTases) were constructed to identify the most effective enzyme combination for indigoidine production. Subsequently, the rate-limiting steps were identified through the supplementation of various precursors. The titer of indigoidine was significantly enhanced through the optimization of its biosynthetic pathway and membrane engineering. Ultimately, a systematic optimization of the fermentation parameters for the engineered BIG33 strain was conducted in shake-flask experiments. As a result, the maximal indigoidine titer reached 4.95 and 26.71 g/L by shake-flask cultivation and fed-batch fermentation, respectively.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133415"},"PeriodicalIF":9.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}