Gwanyeong Ko , Jisung Ryu , Elizabeth Olufunmilayo Oladepo , Jinyoung Chun , Ee Taek Hwang
{"title":"Sustainable recycling of animals rendering waste oil into biodiesel via lipase stabilized in ordered-structured large-pore mesoporous silica derived from rice husk","authors":"Gwanyeong Ko , Jisung Ryu , Elizabeth Olufunmilayo Oladepo , Jinyoung Chun , Ee Taek Hwang","doi":"10.1016/j.biortech.2025.132941","DOIUrl":"10.1016/j.biortech.2025.132941","url":null,"abstract":"<div><div>The disposal of animal rendering waste oil presents substantial environmental challenges, highlighting the need for efficient and sustainable repurposing strategies. This study reports a pioneering enzymatic approach for converting animal rendering waste oil into biodiesel, representing the first reported application of this method. Furthermore, this is the first study to use ordered, structured large-pore mesoporous silica derived from rice husks (OLMS-RH) as an enzyme support, with a ship-in-a-bottle strategy to increase the enzyme stability, reusability, and catalytic efficiency. The immobilized lipase (LP@OLMS-RH) exhibited a 3.91-fold increase in specific activity and marked thermal and pH stability, and retained its performance for nine reaction cycles and 100 days of storage. Under optimized conditions, LP@OLMS-RH achieved a maximum biodiesel yield of 97.0 % within 7 h, significantly surpassing the 61.7 % yield obtained using free lipase. These enhancements were attributed to hyperactivation of the immobilized enzyme, improved substrate accessibility, and tailored microenvironment provided by the OLMS-RH. This study established OLMS-RH as a highly effective enzyme immobilization platform, enabling the development of a durable and reusable catalytic system for biodiesel production. This approach offers a scalable and environmentally sustainable solution to global waste management challenges by simultaneously improving enzyme performance and facilitating waste oil recycling.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132941"},"PeriodicalIF":9.7,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tingan Zhou , Young-Kyoung Park , Rodrigo Ledesma-Amaro
{"title":"Advances in the biosynthesis of β-carotene and its derivatives in yeast","authors":"Tingan Zhou , Young-Kyoung Park , Rodrigo Ledesma-Amaro","doi":"10.1016/j.biortech.2025.132936","DOIUrl":"10.1016/j.biortech.2025.132936","url":null,"abstract":"<div><div>β-Carotene and its derivatives have been gaining huge interest due to its applications as food supplements, nutraceuticals, pharmaceuticals, pigments, etc. Owing to their high values, sustainable microbial production has been a heated research topic. Traditional production methods, such as plant extraction and chemical synthesis, face challenges in scalability, cost, and environmental impact. With advances in synthetic biology, yeast-based biosynthesis has emerged as a promising alternative. This review provides a comprehensive summary of recent progress in the metabolic engineering strategies and fermentation optimization approaches of yeast, particularly <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>, for the production of β-carotene and its derivatives. In contrast to previous reviews, this work emphasizes the shared biosynthetic logic underlying structurally related derivatives, classifying them into two major groups: xanthophylls (canthaxanthin, zeaxanthin, astaxanthin, and violaxanthin) and apocarotenoids (crocetin, retinol, β-ionone, β-cyclocitral, and strigolactones). Representative cases and transferable engineering/fermentation strategies are highlighted. Advantages and limitations of yeast species as production hosts are thoroughly compared, and potential strategies to improve the production are discussed. Future work may focus on broadening product diversity in different yeast hosts and enhancing biosynthetic efficiency for a more sustainable production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132936"},"PeriodicalIF":9.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utilization of industrial by-products as nutrients for gold bioleaching from waste random access memory","authors":"Ngoc Tu Trinh Tran , Duy Tho Tran , Thi Phuong Thuy Pham , Yeoung-Sang Yun","doi":"10.1016/j.biortech.2025.132938","DOIUrl":"10.1016/j.biortech.2025.132938","url":null,"abstract":"<div><div>Gold (Au) recovery from waste random access memory (WRAM) was successfully achieved through a bioleaching process utilizing <em>Chromobacterium violaceum</em>. In this study, condensed distiller solubles (CDS), a by-product of industrial ethanol fermentation, was used to stimulate cyanide production, thereby enhancing Au dissolution. Prior to bioleaching, selective removal of base metals was conducted using 100 mM Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, achieving 99.7 % copper removal without impacting Au content and effectively minimizing competition for cyanide ions. Subsequently, the treated material was used for the Au bioleaching. Bioleaching parameters, including pulp density, CDS concentration, glycine dosage, and pH, were optimized. The highest Au leaching efficiency of 98.6 % was achieved at WRAM pulp density of 10 g/L, CDS concentration of 8 %, glycine concentration of 3 g/L, and pH of 7.0. This integrated strategy, combining selective chemical pretreatment with CDS-enhanced bioleaching, presents a simple, cost-effective, and environmentally sustainable method for efficient Au recovery from electronic waste.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132938"},"PeriodicalIF":9.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lingyi Hou , Boya Liang , Fanzhe Chang, Fang Wang, Shankui Liu, Peng Zhang, Liyun An, Qiang Li
{"title":"Time-series transcriptomic analysis reveals survival strategies of dominant strains in a domesticated community and their influence on polyhydroxyalkanoates synthesis in co-culture","authors":"Lingyi Hou , Boya Liang , Fanzhe Chang, Fang Wang, Shankui Liu, Peng Zhang, Liyun An, Qiang Li","doi":"10.1016/j.biortech.2025.132937","DOIUrl":"10.1016/j.biortech.2025.132937","url":null,"abstract":"<div><div>Mixed microbial cultures are increasingly employed to enhance the production of value-added metabolites like polyhydroxyalkanoates (PHA). However, the role of interspecies interactions in shaping PHA yield and monomer composition remains unclear. In this study, we investigated the impact of co-culturing two PHA-producing strains, <em>Paracoccus shandongensis</em> wg2 and <em>Brevundimonas diminuta</em> R79, which were enriched from activated sludge, on PHA production and gene expression under different carbon sources. When supplied with acetate, the mono-culture of wg2 yielded the highest PHA content, however, co-culturing wg2 with R79, led to reduced PHA accumulation, primarily due to substrate competition. R79 was unable to metabolize propionate and only exhibited transient growth sustained by internal reserves, thereby leaving wg2 as the dominant propionate consumer. Under mixed-carbon conditions, the co-culture demonstrated enhanced production of poly (3-hydroxybutyrate-<em>co</em>-3-hydroxyvalerate) (PHBV) and a higher proportion of 3-hydroxyvalerate (3HV) compared to wg2 mono-cultures, suggesting that interspecies interactions promote monomer diversification. Transcriptomic analysis revealed that co-culture conditions induced differential expression of key metabolic genes in wg2. Specifically, under acetate competition, genes associated with acetate assimilation (<em>acs</em>) and PHA biosynthesis (<em>phaC</em>) were upregulated. In mixed-carbon cultures, genes involved in propionate assimilation (<em>prpE</em>, <em>aspC</em>, <em>leuA</em>) showed increased expression. Overall, our findings underscore that species interactions modulate carbon flux distribution and monomer composition, suggesting that rationally designing microbial communities with complementary metabolic capabilities could serve as a promising strategy to enhance PHA production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132937"},"PeriodicalIF":9.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eid S. Gaballah , Lei Gao , Richen Lin , Mahmoud M. Ali , Biao Yang , Mostafa Sobhi , Chongpeng Tang , Guangcan Zhu
{"title":"Synergistic effects and mechanisms of ultrasonic, pH, and peracetic acid pretreatment on sludge anaerobic fermentation to stimulate short-chain fatty acid production","authors":"Eid S. Gaballah , Lei Gao , Richen Lin , Mahmoud M. Ali , Biao Yang , Mostafa Sobhi , Chongpeng Tang , Guangcan Zhu","doi":"10.1016/j.biortech.2025.132935","DOIUrl":"10.1016/j.biortech.2025.132935","url":null,"abstract":"<div><div>Anaerobic fermentation (AF) for short-chain fatty acid (SCFAs) production from waste-activated sludge (WAS) is a sustainable carbon recovery strategy; however, its efficiency is often constrained by inadequate sludge disintegration. To address this challenge, combined pretreatment methods, including ultrasonic-acidic (US-Ac), ultrasonic-alkali (US-Ak), and ultrasonic-alkali/peracetic acid (US-Ak/PAA) have been proposed as effective solutions. The Results revealed that the US-Ak/PAA pretreatment yielded the highest SCFAs accumulation at 370.5 mg/g VSS at day 6 of AF, surpassing the control, sole PAA, sole pH10, and 0.25US30 reactors by 4.27, 2.7, 2.0, and 1.2-fold, respectively. Furthermore, the US-Ak group (0.25US30) significantly enhanced SCFAs yield by 1.46-fold compared to the US-Ac group (US0.5–30). Mechanistic analysis indicated that the US-Ak/PAA pretreatment effectively disrupted extracellular polymeric substances (EPS), lysed microbial cells, and increased the release of soluble organic matter, thereby optimizing metabolic activity and SCFAs accumulation. The enzymatic activities of protease and α-glucosidase were notably enhanced, demonstrating a strong correlation with improved hydrolysis and acidogenesis processes. Microbial community analysis further revealed that the US-Ak/PAA pretreatment substantially enriched the abundance of hydrolytic and acidogenic bacterial taxa, including <em>Acinetobacter</em>, <em>Petrimonas</em>, <em>Macellibacteroides</em>, <em>Proteiniphilum</em>, <em>Tissierella</em>, and <em>unclassified_c_Clostridia, etc.</em> species<em>.</em> Collectively, the US-Ak/PAA pretreatment strategy not only optimized SCFAs production from WAS but also contributed to mitigating the environmental risks associated with WAS disposal.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132935"},"PeriodicalIF":9.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueqin Lu , Yijing Gao , Xinyu Liu , Yibo Sun , Guangyin Zhen
{"title":"Unlocking microbial community succession and key influencing factors during bioelectrocatalytically-driven simultaneous removal of ammonia nitrogen and sulfate from wastewater","authors":"Xueqin Lu , Yijing Gao , Xinyu Liu , Yibo Sun , Guangyin Zhen","doi":"10.1016/j.biortech.2025.132934","DOIUrl":"10.1016/j.biortech.2025.132934","url":null,"abstract":"<div><div>Ammonia nitrogen (NH<sub>4</sub><sup>+</sup>-N) and sulfate (SO<sub>4</sub><sup>2</sup><sup>−</sup>) removal by Anaerobic ammonium oxidation (Anammox) and sulfate-reducing bacteria (SRB) was studied in dual-chamber microbial electrolysis cells (MECs). Appropriate anode potential stimulation promoted biofilm formation and enhanced extracellular polymeric substances fluorescence, facilitating electron transfer. The highest NH<sub>4</sub><sup>+</sup>-N removal (81.1 %) was achieved at the anode potential of 0.6 V vs. Ag/AgCl after 50 days, coinciding with the increase in electroactive <em>Candidatus_Brocadia</em> from 1.1 % to 27.4 %. Simultaneously, SO<sub>4</sub><sup>2</sup><sup>−</sup> removal reached 77.0 %, supported by cathodic biofilms dominated by SRB (<em>Desulfofustis</em>, <em>Desulfomicrobium,</em> and <em>Desulfatirhabdium</em>). Automated machine learning and principal co-ordinates analysis identified the anode potential as the key factor shaping microbial ecology. The appropriate anode potential (0.4–0.6 V vs. Ag/AgCl) promoted cathodic sulfidogenesis, indirectly enhancing electron flow and supporting Anammox process at the anode. These findings demonstrate that MECs hold great promise for simultaneously enhancing anaerobic ammonia oxidation bacteria and SRB activities, enabling efficient NH<sub>4</sub><sup>+</sup>-N and SO<sub>4</sub><sup>2</sup><sup>−</sup> removal.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132934"},"PeriodicalIF":9.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tobias C. Hull , Eric P. Knoshaug , Robert S. Nelson , Stefaine Van Wychen , Nick Nagle , Jacob S. Kruger , Tao Dong
{"title":"Integrated thermal and biological conversion of microalgal proteins to lipids","authors":"Tobias C. Hull , Eric P. Knoshaug , Robert S. Nelson , Stefaine Van Wychen , Nick Nagle , Jacob S. Kruger , Tao Dong","doi":"10.1016/j.biortech.2025.132927","DOIUrl":"10.1016/j.biortech.2025.132927","url":null,"abstract":"<div><div>Microalgal composition varies with cultivation strategy, and low-cost approaches often produce high-protein biomass. This presents challenges for biorefineries designed around static, lipid-rich feedstocks. In particular, hydrolysates from high-protein algae are nitrogen-rich and sugar-poor, limiting microbial conversion and reducing product yields. This study develops a sequential thermal conditioning and biological upgrading strategy to integrate high-protein hydrolysate processing within conventional lipid extraction and upgrading designs. Oxidative deconstruction was used to break down proteins into ammonium and short-chain carboxylates. Ammonium was subsequently removed to yield a nitrogen-depleted, carboxylate-rich medium suitable for microbial lipid production. Bioconversion trials with <em>Cutaneotrichosporon oleaginosum</em> showed lipid accumulation only from hydrolysates treated with both oxidative deconstruction and nitrogen removal, reaching 1.2 g/L lipids at 30 % intracellular content. This integrated approach enables protein-to-lipid conversion and improves flexibility to process variable algal feedstocks, advancing fuel-oriented microalgal biorefineries.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132927"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linghong Yin , Xingyu Wang , Chen Liang , Jing Liu , Mingbo Wu , Wangda Qu
{"title":"Design and supercapacitor applications of nitrogen, sulfur-codoped carbon foam from over-foaming-prone lignin","authors":"Linghong Yin , Xingyu Wang , Chen Liang , Jing Liu , Mingbo Wu , Wangda Qu","doi":"10.1016/j.biortech.2025.132926","DOIUrl":"10.1016/j.biortech.2025.132926","url":null,"abstract":"<div><div>This study reports a new strategy to regulate the over-foaming of lignin towards carbon foam production. By adding ammonium sulfate ((NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>) to lignin during the thermal conversion process, carbon foam precursor with uniform macrostructure independent of heating conditions are achieved. The mechanism between the interaction of lignin and (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> is discussed, and the resulting nitrogen (N), sulfur (S)-codoped carbon foam exhibits a hierarchical porous structure with compressive strength of 1.5 MPa and several other attractive properties as self-standing electrodes. Benefiting from the continuous and porous carbon network in addition to the N, S co-doping effect, it facilitates efficient electron transport and offers abundant active sites. The L1A2-1000–1-CF electrode in assembled symmetric solid-state supercapacitor device can deliver as high as 9070.9 mF cm<sup>−3</sup> at 2 mV s<sup>−1</sup>. This study provides a facile synthesis of N, S-codoped lignin-derived carbon foam electrodes, highlighting their potential in high-areal capacitance energy storage systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132926"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
João M. Carvalho, Bruno C. Marreiros, Susana P. Gaudêncio, Joana C. Fradinho
{"title":"Valorization of organic rich saline streams by purple phototrophic bacteria mixed cultures through polyhydroxyalkanoates production","authors":"João M. Carvalho, Bruno C. Marreiros, Susana P. Gaudêncio, Joana C. Fradinho","doi":"10.1016/j.biortech.2025.132924","DOIUrl":"10.1016/j.biortech.2025.132924","url":null,"abstract":"<div><div>Saline wastewaters rich in organic compounds present significant disposal management challenges. A cost-effective alternative to expensive treatment methods is their utilization as feedstock for polyhydroxyalkanoates (PHA) production, a biodegradable substitute for traditional plastics, using purple phototrophic bacteria (PPB). This study evaluates the feasibility of using PPB mixed cultures for PHA production under near-seawater salinity conditions (30g<sub>NaCl</sub>.L<sup>−1</sup>), using a synthetic volatile fatty acid mixture comprising C2 to C5 chain lengths. A highly enriched PPB mixed culture (>95 % abundance) was obtained, attaining a PHA content of 24 ± 4 % and a productivity of 0.14 ± 0.02 g<sub>PHA</sub>.L<sup>−1</sup>.day<sup>−1</sup>. Batch assays demonstrated that increased light exposure and continuous availability of C3 to C5 volatile fatty acids enhanced PHA productivity, whereas osmotic shock (45g<sub>NaCl</sub>.L<sup>−1</sup>) negatively impacted PHA production. The obtained PHA productivities align with those reported in non-saline conditions, highlighting the PPB potential for valorizing saline wastewaters through PHA production, within a circular economy framework.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132924"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sadaf-Ilyas Kayani , Feifei Zhu , Zhen Yu , Obaid Ur Rehman , Cunsheng Zhang , Muhammad Abdur Rehman Shah , Yan Li , Shuhao Huo
{"title":"Jasmonates regulate metabolic cascade towards Eicosapentaenoic acid and Docosahexaenoic acid biosynthesis in filamentous microalga Tribonema minus","authors":"Sadaf-Ilyas Kayani , Feifei Zhu , Zhen Yu , Obaid Ur Rehman , Cunsheng Zhang , Muhammad Abdur Rehman Shah , Yan Li , Shuhao Huo","doi":"10.1016/j.biortech.2025.132931","DOIUrl":"10.1016/j.biortech.2025.132931","url":null,"abstract":"<div><div>Microalgae are considered a better alternative to fish and other aquatic resources to obtain essential omega-3 polyunsaturated fatty acids (PUFAs) of human metabolism. <em>Tribonema minus</em> is a fast-growing microalga; however, its natural PUFA content is low. The objective of present study was to increase the content of essential PUFAs in <em>T. minus’</em> oil, to make its commercialization cost-effective. Exogenous methyl jasmonate (MeJA) was used as an elicitor to increase essential PUFAs<em>.</em> Treatment with 10 µM, 20 µM, and 30 µM MeJA was performed. Gas chromatography-mass spectrometry revealed that the concentration of essential omega-3 PUFAs: Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increased by 52-fold and 3.54-fold, respectively, under 10 µM MeJA. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed a 87.5 % increase in PUFA biosynthesis under 20 µM MeJA. Quantitative real-time PCR analysis showed a significant increase in the expression of FA-regulating genes: <em>malonyl-CoA ACP-transacylase</em>, ß-<em>ketoacyl synthase,</em> and <em>Δ12 fatty acid-desaturase</em> under MeJA treatment, as predicted based on JA-binding motifs in their promoter sequences. An increase of 61 % in biomass productivity was obtained under 20 µM MeJA. Chlorophyll concentration and CO<sub>2</sub> fixation increased by 68 % and 59 % under 10 µM and 20 µM MeJA, respectively. A significant increase in the expression of Photosystem I P700 Chlorophyll-a Apoprotein A1 and RuBisCO large subunit was obtained under MeJA treatment. This study provided an efficient method to increase PUFAs’ concentration, and has the potential to produce inexpensive DHA-rich microalgal oil as a food supplement.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132931"},"PeriodicalIF":9.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}