Tingan Zhou , Young-Kyoung Park , Rodrigo Ledesma-Amaro
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Advances in the biosynthesis of β-carotene and its derivatives in yeast
β-Carotene and its derivatives have been gaining huge interest due to its applications as food supplements, nutraceuticals, pharmaceuticals, pigments, etc. Owing to their high values, sustainable microbial production has been a heated research topic. Traditional production methods, such as plant extraction and chemical synthesis, face challenges in scalability, cost, and environmental impact. With advances in synthetic biology, yeast-based biosynthesis has emerged as a promising alternative. This review provides a comprehensive summary of recent progress in the metabolic engineering strategies and fermentation optimization approaches of yeast, particularly Saccharomyces cerevisiae and Yarrowia lipolytica, for the production of β-carotene and its derivatives. In contrast to previous reviews, this work emphasizes the shared biosynthetic logic underlying structurally related derivatives, classifying them into two major groups: xanthophylls (canthaxanthin, zeaxanthin, astaxanthin, and violaxanthin) and apocarotenoids (crocetin, retinol, β-ionone, β-cyclocitral, and strigolactones). Representative cases and transferable engineering/fermentation strategies are highlighted. Advantages and limitations of yeast species as production hosts are thoroughly compared, and potential strategies to improve the production are discussed. Future work may focus on broadening product diversity in different yeast hosts and enhancing biosynthetic efficiency for a more sustainable production.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.