Bioresource Technology最新文献

{"title":"Electrolyte-free electrochemical ammonia stripping integrated with flocculation for efficient nitrogen recovery from swine manure biogas slurry.","authors":"Haorui Zhang, Ahmed Alengebawy, Yuanhao Yang, Zhan Shi, Mohamed Samer, Yanze Zhu, Ping Ai","doi":"10.1016/j.biortech.2025.133440","DOIUrl":"10.1016/j.biortech.2025.133440","url":null,"abstract":"<p><p>A novel electrochemical ammonia stripping (EAS) technology integrated with flocculation was developed to effectively remove and recover ammonia nitrogen from swine manure biogas slurry (SMBS) without alkali addition or heating. The optimal operational conditions were established by the suspended solids (SS) removal rate, the ammonia nitrogen removal rate (ANRR), and recovery content. The maximum SS removal rate (66 %) was achieved by adding 4 g/L of cationic polyacrylamide at pH 7 with stirring at 1000 rpm for 20 min. The pH of SMBS in the cathode could be raised beyond 12, facilitating the ammonia nitrogen removal and its recovery as (NH₄)₂SO₄. The cathode feeding mode performed better than the anode feeding mode, increasing the ANRR from 57 % to 86 %, and a corresponding enhancement in the recovery content from 172 to 414 mg/kg. Specifically, under a 35 mA/cm² applied current density, 0.9 L/min aeration flow rate, and 40 % absorbed sulfuric acid concentration, the ANRR reached 91 %, with a recovery content of 1151 mg/kg. The electrolyte-free configuration avoids interference from competing cations, enhancing NH₄⁺ transport and improving ANRR (>90 %), with a recovery content of 1784 mg/kg at an applied current density of 25 mA/cm². The economic assessment revealed that the electrolyte-free EAS system exhibited the lowest energy consumption at 0.5 kWh/kg N and a cost of $0.06/kg N, representing only 16.5 % of the expenses associated with conventional ammonia stripping. These results confirm the potential of EAS as a sustainable and eco-friendly technology for ammonia nitrogen recovery.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133440"},"PeriodicalIF":9.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach for utilizing wild plant resources in green agricultural production: A case study of preparing agricultural Jiaosu from Humulus scandens","authors":"Meng Li ,&nbsp;Tingru Yue ,&nbsp;Wancong Luo ,&nbsp;Juanqi Li ,&nbsp;Hu Wang ,&nbsp;Xueting Wang ,&nbsp;Qingjie Du ,&nbsp;Jiqing Wang ,&nbsp;Huaijuan Xiao","doi":"10.1016/j.biortech.2025.133434","DOIUrl":"10.1016/j.biortech.2025.133434","url":null,"abstract":"<div><div>Wild plant resources possess significant potential for green agricultural production, yet their utilization pathways remain limited. As an eco-friendly technology, agricultural Jiaosu (AJ) offers a promising approach to enhance sustainable agriculture. This study investigated the feasibility of preparing AJ from wild plants. Five species—<em>Humulus scandens</em>, <em>Gynostemma pentaphyllum</em>, <em>Rumex dentatus</em>, <em>Euphorbia helioscopia</em>, and <em>Portulaca oleracea</em>—were processed into AJ, which exhibited abundant organic matter and mineral nutrients. Using <em>Humulus scandens</em> AJ as a representative example, the AJ solution contained rich organic substances, including rhamnose (543.9 mg/L), tyrosine (178.3 mg/L), methionine (110.1 mg/L), alanine (105.9 mg/L), acetic acid (9.0 g/L), butyric acid (6.6 g/L), lactic acid (4.9 g/L), along with phytohormones such as auxin (191.9 μg/L), salicylic acid (167.0 μg/L), and jasmonic acid (123.4 μg/L). Comparative trials with 1/2 Hoagland solution (CK) demonstrated that <em>Humulus scandens</em> AJ application achieved comparable effects on pakchoi growth and nutritional quality. Notably, AJ treatment enhanced root development and increased vitamin C, flavonoids, anthocyanins, and total phenolics compared to the control. Mechanistic analysis indicated that AJ application contributed to cultivate soil fertility by elevating soil organic matter, mineral nutrient, and enzymatic activities, altering soil secondary metabolites and restructuring microbial communities. Comprehensive analysis identified 22 environmental factors significantly influencing soil microbiota; AJ could directly or indirectly improve soil ecological conditions to enhance pakchoi root development and quality formation. This study demonstrated that AJ technology could effectively convert wild plants, including undesirable species, into valuable agricultural inputs. By restructuring soil microecosystems, AJ promoted sustainable agricultural development.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133434"},"PeriodicalIF":9.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated base editing and microfluidics boost microbial lipid production from lignin.","authors":"Changyu Pi, Jinyang Li, Tongtong Bao, Le Gao, Xin Wu","doi":"10.1016/j.biortech.2025.133441","DOIUrl":"10.1016/j.biortech.2025.133441","url":null,"abstract":"<p><p>Lignin, a recalcitrant aromatic biopolymer, is a promising feedstock for sustainable valorization. Here, we investigated the transcriptional response of Curvularia clavata J1 to alkali lignin (AL) and established a Cu²⁺-inducible MCM5-AID base editor for genome-wide C-to-T/G-to-A mutagenesis. Transcriptomics revealed AL-induced upregulation of electron transport, lipid catabolism, and iron homeostasis. Droplet microfluidics enabled ultrahigh-throughput screening, identifying mutant M6 with superior traits. M6 achieved 49 % lipid of dry cell weight (DCW) (36 % increase) and 228.58 U/L laccase activity (75 % increase) in shake flasks, further validated in bioreactors with 51 % DCW lipid (33 % increase) and 240.43 U/L laccase activity (87 % increase). Genome sequencing confirmed random C-to-T mutations, while transcriptomics indicated enhanced fatty acid, steroid, and glycerolipid biosynthesis redirecting carbon flux to oil-enriched single-cell protein. This work provides an integrated framework for lignin biotransformation and highlights the potential of synthetic biology-microfluidics integration for precise fungal engineering.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133441"},"PeriodicalIF":9.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formate modulation of nitrite-oxidizing bacteria metabolism for rapid nitritation in municipal wastewater treatment.","authors":"Xinjie Gao, Zeming An, Baishuo Shao, Xinsheng Fan, Yongzhen Peng","doi":"10.1016/j.biortech.2025.133435","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133435","url":null,"abstract":"<p><p>Improving the removal of nitrogen from municipal wastewater through sustainable methods is crucial for mitigating global nitrogen pollution. In this study, nitritation was rapidly achieved within 8 days in municipal wastewater treatment through the formate addition, with the nitrite accumulation rate and total nitrogen removal efficiency being stably maintained at 76 % and 93 %, respectively. In contrast, the control group receiving an equivalent amount of acetate failed to achieve nitritation, despite exhibiting a lower abundance of nitrite-oxidizing bacteria (NOB). Moreover, DNA-based stable isotope probing, oxygen uptake rates, and transcriptome analysis suggested that the preferential use of oxygen or nitrate by NOB to oxidize formate could be the underlying mechanism for nitritation. Compared with other carbon sources, the formate addition achieved superior removal of nitrogen with lower consumption of carbon. Overall, nitritation offers a practical pathway for integration with anaerobic ammonium oxidation for more sustainable and advanced nitrogen removal from real wastewater.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133435"},"PeriodicalIF":9.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reusing bioreactor effluent as a circular bioresource strategy for cultivating marine microalgae and producing Omega-3 polyunsaturated fatty acids.","authors":"Sara Swa Thi, Angel Anisa Cokro, Ezequiel Santillan, Tiago Toscano Selão, Ramanujam Srinivasan Vethathirri, Rebecca Josephine Case, Stefan Wuertz","doi":"10.1016/j.biortech.2025.133433","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133433","url":null,"abstract":"<p><p>The rapid growth of the aquaculture industry underscores the need for sustainable alternatives for traditional fishmeal. We investigated the potential of reusing treated effluent as a circular bioresource strategy. Effluent from two bioreactors producing single-cell protein (SCP), fed with soybean-processing wastewater, was used to cultivate omega-3 polyunsaturated fatty acid (PUFA)-producing marine microalgae. Marine microalgae strains were cultivated in marine growth medium amended with varying percentages of autoclaved bioreactor effluent (BE). The effects of lowered salinity, due to the addition of effluent, and temperature on chlorophyll a, a proxy for biomass, and omega-3 PUFAs content were assessed. We found that Dunaliella tertiolecta and Nannochloropsis oculata can grow in marine medium containing 85 % autoclaved BE (corresponding to 5.3 ppt salinity); Tisochrysis lutea grows in marine medium containing 50 % BE (15.3 ppt salinity); and a Singaporean strain of Nannochloropsis oceanica grows in 10 % BE (25.5 ppt salinity). The PUFAs alpha-linolenic acid (ALA), docosahexaenoic acid (DHA), and/or eicosapentaenoic acid (EPA) were also synthesized by the strains when grown in their respective optimized media formulations. Lower growth temperatures (15 °C and/or 18 °C) led to higher production of omega-3 PUFAs in all four strains. This proof-of-concept study demonstrates that some microalgal strains accumulate DHA, EPA and/or ALA when grown in medium containing autoclaved effluent from an SCP-producing bioreactor and may potentially be used as a supplement to aquafeed. This approach promotes resource efficiency, supports a circular bioeconomy, and integrates algal biotechnology with microbial protein systems for sustainable feed production and wastewater reuse.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133433"},"PeriodicalIF":9.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycolytic flux increase in Lactococcus cremoris coincides with accelerated pathway decay and reduced cumulative product yield.","authors":"Avis Dwi Wahyu Nugroho, Michiel Kleerebezem, Herwig Bachmann","doi":"10.1016/j.biortech.2025.133427","DOIUrl":"10.1016/j.biortech.2025.133427","url":null,"abstract":"<p><p>Non-growing cells are commonly encountered in nature and often desired in biotechnological applications to maximize product yields. Such cells exhibit limited protein synthesis, and their metabolic functionality relies on the long-term stability and repair of enzymes and pathways to sustain metabolic activity. However, knowledge of the factors that influence prolonged metabolism is lacking. A biotechnological example is the production of lactic acid. Here we show that prolonged lactic acid formation in non-growing, translationally blocked cells, is not constrained by the intrinsic maximum number of catalytic cycles, but by the metabolic flux. Faster conversion coincided with faster pathway decay and importantly lower cumulative product yield, and vice versa. This behavior is consistent irrespective of whether the flux is altered through manganese addition, changing the cellular ATP demand, or enzyme expression levels. The correlation between flux and pathway decay is relevant for biotechnological applications, and the fitness of growth arrested environmental microorganisms.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133427"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of sterile and non-sterile solid-state bioprocessing using Lichtheimia ramosa for agro-industrial waste valorization.","authors":"Kemylli Farinon, César Milton Barato, Alison Likoski Neves, Rodrigo Simões Ribeiro Leite, Jane Mary Lafayette Neves Gelinski, Gustavo Graciano Fonseca","doi":"10.1016/j.biortech.2025.133429","DOIUrl":"10.1016/j.biortech.2025.133429","url":null,"abstract":"<p><p>This study investigated the biotechnological potential of Lichtheimia ramosa in the solid-state bioprocessing (SSB) of various agro-industrial and agricultural residues under both sterile and non-sterile conditions. Decomposing wood sawdust was used as the primary substrate, supplemented with different co-substrates including wheat bran, animal manures, slaughterhouse sludge, dairy wastes, apple pomace, and urea. The 28-day SSB experiments revealed significant changes in substrate composition, with notable increases in nitrogen content (up to 34.58 % in non-sterile wheat bran treatment) and corresponding reductions in C:N ratios, indicating enhanced organic matter mineralization. Enzymatic activity profiles demonstrated L. ramosa's robust lignocellulolytic capabilities, with substrate-specific enzyme induction patterns observed. Under sterile conditions, wheat bran supplementation yielded the highest enzymatic activities, with CMCase reaching 0.71 U/g, β-glucosidase 0.82 U/g, and amylase 7.85 U/g. In non-sterile systems, cooperative microbial interactions were evident, with xylanase activity peaking earlier (2.31 U/g at day 7) than in sterile conditions, suggesting preferential hemicellulose degradation by the mixed microbiota. The study revealed that system sterility, substrate composition, and nitrogen source interactively influenced hydrolysis efficiency and enzyme production. Wheat bran, slaughterhouse sludge, and fresh dairy solids emerged as the most effective co-substrates for optimizing bioconversion processes. These findings highlight L. ramosa's versatile biocatalyst potential for waste valorization, with sterile systems recommended for targeted lignocellulase production and non-sterile systems beneficial for organic waste mineralization.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133429"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudocapacitive MXene@Fe-TA ternary mediator enhances denitrification via optimized electron transfer and microbial regulation in wastewater treatment","authors":"Sihao Pan ,&nbsp;Xiaoping Wang ,&nbsp;Tingting Guo ,&nbsp;Hongxue An ,&nbsp;Yankai Guo ,&nbsp;Zhi Chen ,&nbsp;Jing Lian ,&nbsp;Jianbo Guo","doi":"10.1016/j.biortech.2025.133420","DOIUrl":"10.1016/j.biortech.2025.133420","url":null,"abstract":"<div><div>Inefficient electron transfer is one of the main challenges in practical denitrification applications. To address this issue, a novel ternary mediator functional material (Mxene@Fe-TA) was successfully synthesized, which exhibited pseudocapacitive properties to facilitate electron mediation and distribution. Batch experiments revealed that 50 mg/L MXene@Fe-TA achieved 2.04-fold nitrate degradation. This superior performance originated from three integrated mechanisms: (1) MXene@Fe-TA served as a pseudocapacitor, which stored electrons from substrate metabolism by electrochemical structure; (2) it fostered a microenvironment that selectively enriched electroactive bacteria (EAB), which boosted secretion of redox-active substances to accelerate extracellular electron transfer (EET); and (3) the battery function of MXene@Fe-TA facilitated the stored electrons flowed directionally to denitrification, thereby optimizing energy utilization and reducing metabolic demands. This study introduced an innovative strategy for denitrification and guided the design of multifunctional materials to improve the efficiency of biological wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133420"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae biorefinery in the belt and road initiative: opportunities for green growth.","authors":"Zimeng Zhang, Dingkun Lei, Jin Yang, Ziyu Liu","doi":"10.1016/j.biortech.2025.133428","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133428","url":null,"abstract":"<p><p>In the context of global energy transformation and climate change, the microalgae industry has emerged as a strategic avenue for promoting green development, driven by its energy conservation, emission reduction, and resource recycling advantages. As a key advocate of the Belt and Road Initiative (BRI), China has increasingly integrated green development into its cooperation framework, fostering the application of microalgae technology in BRI countries and contributing to achieving both environmental and economic goals. This paper reviews the advancements in microalgae technology within bioenergy, high-value product development, and environmental sectors. This review differs from existing studies by systematically linking technological advances in microalgae biorefining with the BRI framework. It examines the entire biorefinery chain-from cultivation to product valorization-while emphasizing improvement measures at each stage, including hybrid cultivation systems to reduce costs, advanced extraction methods to enhance yields, and integrated biorefinery models to optimize resource efficiency. A technology-market matching analysis further reveals that while biofuels face high demand but limited technological maturity, high-value products such as astaxanthin and EPA/DHA show stronger alignment with current market growth. Future efforts should focus on establishing joint pilot-scale facilities, harmonizing green trade standards, and strengthening multilateral cooperation under the BRI. By combining technological innovation with international collaboration, microalgae biorefining can become a key driver of sustainable economic transformation and contribute to achieving global low-carbon goals.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133428"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel Dye-Decolorizing peroxidase from Brevibacillus agri and its application in detoxification of textile industry wastewater","authors":"Wenjing Lin ,&nbsp;Wei Lin ,&nbsp;Lizhen Yang ,&nbsp;Chunfang Zhang ,&nbsp;Xuehui Li ,&nbsp;Jianbin Xiao ,&nbsp;Xingtong Chen ,&nbsp;Fan Cai ,&nbsp;Chao Chen ,&nbsp;Mingliang Zhang ,&nbsp;Yan Zhang ,&nbsp;Li Li","doi":"10.1016/j.biortech.2025.133430","DOIUrl":"10.1016/j.biortech.2025.133430","url":null,"abstract":"<div><div>Dye-containing wastewater from the textile industry poses severe ecological and health risks. Here, we identified and characterized a novel dye-decolorizing peroxidase (BaDyP) from <em>Brevibacillus agri</em>, notable for its broad substrate range and catalytic robustness. Heterologous expression in <em>Escherichia coli</em> yielded superior activity compared to <em>Saccharomyces cerevisiae</em>. Recombinant BaDyP efficiently decolorized seven structurally diverse dyes, achieving removal rates of up to 96.7 % (Reactive Black 5, D240E variant), 95.0 % (Congo Red), and 93.2 % (Methylene Blue). BaDyP also decolorized authentic industrial effluent with 78.1 % efficiency, which was further enhanced to 93.3 % by the D240E mutant, representing a 19.5 % improvement. Importantly, BaDyP treatment reduced wastewater toxicity, as demonstrated by microbial growth assays, and structural dye degradation was validated by FTIR. Collectively, these findings highlight BaDyP as a promising biocatalyst for sustainable remediation of dye-contaminated industrial effluents.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"440 ","pages":"Article 133430"},"PeriodicalIF":9.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}