Bioresource Technology最新文献

{"title":"Homoacetogenic bioaugmentation with Clostridium magnum WY-4 enhances upflow anaerobic sludge blanket reactor resilience under progressive organic loading and short-term complex shock.","authors":"Vladimir Burov, Jianzheng Li, Jia Meng","doi":"10.1016/j.biortech.2026.136075","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136075","url":null,"abstract":"<p><p>Upflow anaerobic sludge blanket (UASB) reactors are widely used for treating high-strength organic wastewater but are vulnerable to short-term complex shocks caused by increased organic loading, hydraulic retention time regulation, and partial sludge loss. At the same time, the role of homoacetogenic bioaugmentation in improving UASB recovery after such disturbances remains poorly understood. This study evaluated the potential of a novel homoacetogenic strain, Clostridium magnum WY-4 (WY-4), to enhance UASB resilience. Two laboratory-scale UASB reactors were operated for 184 days, and reactor performance, volatile fatty acids (VFA), extracellular polymeric substances (EPS), N-acyl-homoserine lactones (AHL), and microbial community dynamics were analyzed. Under stable conditions, WY-4 had only a limited effect on reactor performance. During and after the shock, however, bioaugmentation markedly accelerated recovery. The maximum effluent VFA concentration in the WY-4 reactor during the shock reached 190 mg/L, 53% lower than in the control. Propionate degradation was enhanced, with only trace amounts remaining in the bottom zone of the WY-4 reactor, whereas acetate and propionate continued to accumulate in the control. During the first nine days of recovery, methane yield was approximately 20% higher in the WY-4 reactor. Principal component analysis showed a faster recovery of AHL profiles, accompanied by altered AHL-EPS relationships. Although dominant microbial populations changed little, the WY-4 reactor maintained greater microbial richness and diversity. The homoacetogenic bioaugmentation with WY-4 enhanced UASB resilience by reducing VFA accumulation, accelerating methane recovery, and promoting restoration of AHL-mediated quorum sensing following short-term complex shock.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136075"},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into riboflavin-mediated recovery of the single-stage partial nitritation-anammox system under tetracycline stress.","authors":"Liping Liu, Lingjie Liu, Chunsheng Qiu, Jingjie Yu, Chenchen Wang, Shaopo Wang","doi":"10.1016/j.biortech.2026.136039","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136039","url":null,"abstract":"<p><p>Tetracycline (TC) contamination poses a potential threat to the stability of anammox-based wastewater treatment systems, yet effective strategies for accelerating functional recovery remain poorly understood. This study explored the inhibitory effects of 5 mg/L TC on a single-stage partial nitritation-anammox (SPNA) system, and further unraveled its functional potential and underlying mechanisms of exogenous riboflavin for restoring system performance. TC exposure substantially impaired nitrogen removal, with total nitrogen removal efficiency decreasing from 53.39 % to 34.44 %, accompanied by a pronounced decline in anammox activity. Supplementation with 0.5 mg/L riboflavin accelerated system recovery, partially restoring total nitrogen removal efficiency to 48.15 %, by enhancing anammox activity and suppressing nitrite-oxidizing bacteria proliferation. Riboflavin alleviated these disturbances by enhancing electron transfer, mitigating oxidative stress, and restoring nitrogen metabolism-related functional genes. Metagenomic analysis further revealed that riboflavin recovered extracellular electron transfer and amino acid and nucleotide metabolism pathways disrupted by TC stress, while promoting the enrichment of tetracycline resistance and degradation-related genes and putative tetracycline-degrading bacteria, including Sphingobacterium and Bacillus. These responses were accompanied by enhanced tetracycline removal, suggesting potential mechanisms of improved microbial adaptation to antibiotic stress.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136039"},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Headspace-driven metabolic rerouting balances chain elongation and acetogenic pathways for high-efficiency carbon recovery.","authors":"Wenyan Zhao, Yuxuan Liao, Weixiang Fan, Youli Yang, Binghua Yan, Jonathan W C Wong","doi":"10.1016/j.biortech.2026.136072","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136072","url":null,"abstract":"<p><p>In situ assimilation of off-gases (H₂/CO₂) into liquid metabolites during anaerobic fermentation presents a promising approach to maximize waste biomass valorization. However, mechanisms governing the interaction between microbial carbon fixation and chain elongation (CE) through headspace dynamics remain unclear. This study explored gas-liquid metabolic flux and microbial response by varying headspace pressure/composition in batch CE bioreactors. Autogenic pressure reshaped the physicochemical microenvironment, which potentially induced specific biological inhibition and constrained ethanol utilization. Accordingly, caproic acid production decreased by 40.50-51.68%, whereas acetic acid accumulation increased by 70.67-104.44% under both N₂ and H₂/CO₂ headspace. This alteration reflected metabolic flux redistribution between CE and acetic acid-producing pathways driven mainly by electron donor oxidation with homoacetogenesis as a potential secondary contributor. Meanwhile, an initial H₂/CO₂ headspace curbed off-gas emissions and induced a final 17.62 kPa (abs) vacuum, enhancing soluble metabolite carbon retention. Consequently, liquid-phase carbon recovery increased to 84.29% under autogenic pressure and peaked at 89.68% following further H₂/CO₂ headspace replacement. Microbial profiling revealed that self-pressurized conditions suppressed Oscillibacter from 15.14% to 1.93%, whereas H₂/CO₂ exposure selectively enriched Caproiciproducens to 30.52% as the dominant CE driver. Metatranscriptomics demonstrated that autogenic pressure markedly upregulated the Wood-Ljungdahl pathway genes by up to 28.62-fold, accompanied by induction of stress-responsive genes for physiological adaptation. Additionally, the initial H₂/CO₂ headspace promoted the expression of gene korA, suggesting a potential partial reductive tricarboxylic acid (rTCA) pathway for carbon fixation. These insights provide a mechanistic framework for optimizing carbon recovery and product selectivity in engineered CE systems, advancing sustainable waste-to-biochemical platforms.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136072"},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ureolytic bacteria-loaded modified biochar microcapsules for enhanced cadmium immobilization under acid conditions.","authors":"Xiang Lai, Peng Zhu, Yan Wang, Junjie Zheng, Jiajie Tang, Wenbing Yang","doi":"10.1016/j.biortech.2026.136064","DOIUrl":"10.1016/j.biortech.2026.136064","url":null,"abstract":"<p><p>Cadmium contamination in acidic environments remains a critical challenge due to enhanced metal mobility and the limited efficiency of conventional remediation technologies. Microbially induced carbonate precipitation (MICP) is a promising sustainable approach for heavy metal immobilization; however, its application under acidic conditions is severely restricted by reduced microbial activity and insufficient carbonate generation. Modified biochar was used as a functional carrier to develop adsorption-based microcapsules (AM) and entrapment-based microcapsules (EM) loaded with ureolytic bacteria for Cd²⁺ immobilization in acidic environments. Results showed that EM effectively protected bacteria under acid stress and maintained high urease activity, and Cd²⁺ removal achieved over 99.7 % in aqueous solutions, much greater than other groups at pH 3. The unconfined compressive strength of solidified soil increased with curing age and pH. At pH 7 and 14 curing days, AM exhibited the highest strength, whereas EM showed lower strength due to the limited mechanical stiffness of sodium alginate hydrogel beads. Both microcapsules reduced Cd²⁺ leaching, EM achieving the greatest reduction, 37.3 % lower than the control group. Cadmium immobilization was attributed to exchangeable Cd transformed into carbonate-bound and residual fractions through surface adsorption, complexation, and carbonate coprecipitation. The results of this study offer valuable insights for the remediation of heavy metal contaminated sites, especially in acidic environments.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136064"},"PeriodicalIF":8.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halide-mediated electrochemical functionalization of lignin for high-performance flexible humidity sensors.","authors":"Busarakham Ngokpho, Thitima Treekaew, Jiranan Srikaeothiang, Pattanaphong Janphuang, Pimchanok Leuasoongnoen, Pattarawadee Therdkatanyuphong, Chanida Jakkrawhad, Supinya Nijpanich, Phanawan Whangdee, Kamonwad Ngamchuea","doi":"10.1016/j.biortech.2026.136068","DOIUrl":"10.1016/j.biortech.2026.136068","url":null,"abstract":"<p><p>Lignin is an abundant, renewable aromatic biopolymer that remains largely underutilized despite its rich chemical potential. In this work, lignin is valorized through a mild electrochemical functionalization process using chloride ions as a redox mediator on a boron-doped diamond electrode. This halide-mediated oxidation introduces oxygen-containing functional groups, while simultaneously inducing particle fragmentation and increasing surface accessibility. These structural modifications significantly enhance the hydrophilicity and interfacial interaction of lignin with gold electrodes. The electrochemically oxidized lignin is combined with polyvinylpyrrolidone (PVP) and subsequently integrated as the active sensing layer in a resistive humidity sensor fabricated on flexible Au/Cr interdigitated electrodes. The resulting sensor achieves a high humidity response of up to 2.0 × 10⁷% over a broad relative humidity range (33-90% RH). The device exhibits an optimal dynamic operating range between 40% and 90% RH, featuring fast response and recovery times of 0.2  s. Kinetic limitations are observed at the lower humidity (ca. 200  s at 33% RH) as the isolated water monolayers lack continuous hydrogen-bonding required to facilitate rapid Grotthuss proton transport. Through this approach, we demonstrate an effective pathway for advanced biomass valorization in functional, moisture-responsive flexible electronics.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136068"},"PeriodicalIF":8.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organophosphate esters differentially impair soil phosphorus cycling: mechanistic insights and remediation strategies.","authors":"Xin Qi, Ziwei Wang, Xin Huang, Fan Liu, Guiyin Wang, Changlian Xu, Guochun Lv, Junzhuo Cai, Zhang Cheng, Zhanbiao Yang, Liling Wang, Xiaoxun Xu","doi":"10.1016/j.biortech.2026.136055","DOIUrl":"10.1016/j.biortech.2026.136055","url":null,"abstract":"<p><p>Organophosphate esters (OPEs) are widespread soil contaminants, yet their effects on microbially driven phosphorus (P) cycling remain poorly understood. Here, we conducted 90-day microcosm experiments using agricultural soil spiked with triphenyl phosphate (TPhP, an aryl-OPE) or tris(1-chloro-2-propyl) phosphate (TCPP, a halogenated OPE) to investigate their effects on microbially mediated P cycling. After 90 days, TCPP decreased available P by 12-26% compared to CK and exerted stronger microbial suppression than TPhP at high concentrations, disrupting co-occurrence networks, causing keystone taxa niche loss, and broadly suppressing C-P cycling genes. High-concentration TCPP significantly perturbed microbial lipid metabolism, specifically linoleic acid metabolism and unsaturated fatty acid biosynthesis. In contrast, TPhP, especially at low exposure levels, enriched key P-cycling taxa (e.g., Massilia, Sphingomonas, and Cladosporium) and phosphatase-encoding genes, thereby accelerating available P release. Bioaugmentation experiment indicated that the SynCom treatment consisting of two indigenous keystone degraders, Sphingomonas sp. OP1 and Pseudomonas sp. OP2, increased TPhP and TCPP removal by 26.1% and 16.1%, respectively, compared with the corresponding non-inoculated treatment at 60 days. These findings demonstrate that OPEs regulate soil P cycling through concentration- and structure-dependent mechanisms and identify indigenous SynComs as a promising bioremediation strategy for OPE-contaminated soils.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136055"},"PeriodicalIF":8.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substrate-mediated facilitation of softwood and hardwood deconstruction by sequential white-rot and brown-rot fungal treatment.","authors":"Dongcheng Wu, Yan Li, Chenpu Huang, Xiyuan Chen, Xiazhen Li, Yuan Zhu, Xianjun Li","doi":"10.1016/j.biortech.2026.136067","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136067","url":null,"abstract":"<p><p>Translating natural, successional wood decomposition into an engineered two-stage fungal pretreatment offers a promising strategy for biomass deconstruction, yet how pioneer white-rot fungal modifications facilitate subsequent brown-rot decay remains unclear. Scots pine (Pinus sylvestris L.) was pretreated with Trametes versicolor (Tv) and Phanerochaete chrysosporium (Pc) and subsequently exposed to Gloeophyllum trabeum (Gt). After 15-day BRF incubation, second-stage mass loss increased by 40.9% and 126.2% in the Tv- and Pc-pretreated groups, respectively, relative to the control without WRF pretreatment. FTIR, chemical-composition and qualitative HSQC-NMR analyses collectively indicated that this accelerated degradation of Scots pine was associated with the WRF-induced modification of the lignin-carbohydrate matrix. Furthermore, this substrate-mediated facilitative effect was also observed in red oak (Quercus rubra L.), for which second-stage mass loss reached 13.1% and 14.7% after P. chrysosporium and T. versicolor pretreatment, respectively, compared with 6.7% in the control. Under the fungal combinations and laboratory conditions tested, these results show that WRF-induced substrate modification can facilitate subsequent brown-rot deconstruction and provide a basis for further exploring sequential fungal treatments in woody biomass.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136067"},"PeriodicalIF":8.2,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual roles of multi-walled carbon nanotubes in anammox systems: Conductive enhancement or toxic burden?","authors":"Xingcheng Zhao, Bo Wang, Fangxu Jia, Xu Zhang, Yawen Liu, Hong Yao","doi":"10.1016/j.biortech.2026.136065","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136065","url":null,"abstract":"<p><p>Integrating multi-walled carbon nanotubes (MWCNTs) into anaerobic ammonium oxidation (anammox) systems offers a promising nitrogen removal strategy, yet the balance between conductive stimulation and nanotoxicity remains poorly understood. This study elucidated the dose-dependent mechanisms of MWCNTs on anammox consortia. A distinct biphasic response was observed: low doses (optimal 30 mg·L^-1) enhanced specific anammox activity by 18.82 ± 1.73% (p < 0.001), whereas high doses (≥ 100 mg·L^-1) caused severe inhibition. Mechanistically, 30 mg·L^-1 MWCNTs stimulated EPS to physically intercept nanoparticles while triggering an adaptive antioxidant response. Metatranscriptomic revealed that MWCNTs acted as \"conductive bridges\" to reprogram microbial electron flow, thereby selectively favoring AnAOB, promoting endogenous electron production and transfer, and ultimately enhancing the turnover of key terminal enzymes (hzs, hdh). Conversely, excessive exposure overwhelmed EPS and antioxidant defenses, causing reactive oxygen species accumulation and cellular disruption. Long-term operation confirmed sustained performance, expanding the AnAOB ecological niche (21.72 ± 9.62% increase) while suppressing competing denitrifiers. These findings provide a comprehensive molecular framework for precisely regulating nanomaterial dosing to optimize advanced wastewater treatment while mitigating ecological risks.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136065"},"PeriodicalIF":8.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ball-milling medium dictates the reconstruction pathway of hydrochar: Coupling pore opening with functionality retention.","authors":"Xin Huang, Chi Zhu, Guixian Xie, Huiqiang Ma, Bo Wang","doi":"10.1016/j.biortech.2026.136066","DOIUrl":"10.1016/j.biortech.2026.136066","url":null,"abstract":"<p><p>Hydrochar retains abundant oxygen-containing functional groups but little porosity, and thermal upgrading opens pores at the expense of surface chemistry. Ball milling requires no external heating, so its structural outcome is decided by how freshly fragmented particles reorganize. Here, dry and water-mediated ball milling of corn-straw hydrochar were compared to resolve how the milling medium directs this reorganization, with 600℃ biochar as a thermal reference. Without a liquid phase, fragments recontacted and densified: the specific surface area fell from 11.23 to 2.91 m²·g^-1, the total pore volume from 0.0818 to 0.0179 cm³·g^-1, and the CO proportion from 26.2 % to 19.0 %, so pore closure and carbonyl loss occurred together. In water, dispersion suppressed reagglomeration, increasing the specific surface area, total pore volume, and CO proportion to 34.26 m²·g^-1, 0.1903 cm³·g^-1, and 33.3 %, respectively. HTC-WBM adsorbed 2.53, 2.41, and 3.15 times as much TC as HTC, HTC-DBM, and BC, respectively. Although BC had the highest specific surface area (54.99 m²·g^-1), its total pore volume and BET-equivalent average pore diameter were only 0.0439 cm³·g^-1 and 3.19 nm, compared with 0.1903 cm³·g^-1 and 22.22 nm for HTC-WBM. Open meso-/macropores facilitated TC transport to retained oxygen-containing sites, where hydrogen bonding and electrostatic interactions contributed to adsorption. Spectroscopic evidence and SHAP analysis were consistent with this coupled pore-accessibility/functionality effect. Medium-controlled milling therefore couples pore opening with functionality retention.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136066"},"PeriodicalIF":8.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced nitrogen removal from aquaculture wastewater by biochar-assisted algal-bacterial bioaugmentation using direct ammonium-oxidizing and heterotrophic nitrification-aerobic denitrifying bacterial consortium.","authors":"Yuwei Mi, Linna Xie, Qixing Hu, Dongbo Liu, Yijie Zheng, Zhiquan Hu","doi":"10.1016/j.biortech.2026.136062","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136062","url":null,"abstract":"<p><p>Efficient nitrogen removal from low-C/N aquaculture wastewater remains challenging because carbon availability can constrain nitrogen conversion. A biochar-assisted algal-bacterial system was developed using a consortium capable of direct ammonium oxidation (Dirammox) and heterotrophic nitrification-aerobic denitrification. Four sequencing batch reactors were operated to compare the individual and combined effects of bioaugmentation and biochar. The combined system achieved a maximum total nitrogen (TN) removal efficiency of 88 % and a mean stable effluent TN concentration of 2.8 mg·L^-1, compared with 7.2 mg·L^-1 in activated sludge (AS). Bioaugmentation was associated with greater extracellular polymeric substance accumulation and intracellular metabolic activity, whereas biochar was associated with changes in floc-carrier characteristics and interfacial electrochemical properties. The combined system exhibited a charge-transfer resistance of 18.7 kΩ, only 7 % of that in AS, and an electron-exchange capacity of 3.7 μmol e^-·g^-1. Functional profiles indicated the coexistence of conventional nitrification with Dirammox-associated and aerobic denitrification potential, with dnfA reaching 6.6 × 10⁸ copies·mg^-1 suspended solids, 5.3-fold that in AS. These findings suggest that combining this bacterial consortium with biochar can improve nitrogen removal through complementary nitrogen-transformation routes and enhanced interfacial electron exchange, providing design guidance for biochar-assisted algal-bacterial treatment of aquaculture wastewater.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136062"},"PeriodicalIF":8.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}