Formate modulation of nitrite-oxidizing bacteria metabolism for rapid nitritation in municipal wastewater treatment

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-10-02 DOI:10.1016/j.biortech.2025.133435

Xinjie Gao , Zeming An , Baishuo Shao , Xinsheng Fan , Yongzhen Peng

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引用次数: 0

Abstract

Improving the removal of nitrogen from municipal wastewater through sustainable methods is crucial for mitigating global nitrogen pollution. In this study, nitritation was rapidly achieved within 8 days in municipal wastewater treatment through the formate addition, with the nitrite accumulation rate and total nitrogen removal efficiency being stably maintained at 76 % and 93 %, respectively. In contrast, the control group receiving an equivalent amount of acetate failed to achieve nitritation, despite exhibiting a lower abundance of nitrite-oxidizing bacteria (NOB). Moreover, DNA-based stable isotope probing, oxygen uptake rates, and transcriptome analysis suggested that the preferential use of oxygen or nitrate by NOB to oxidize formate could be the underlying mechanism for nitritation. Compared with other carbon sources, the formate addition achieved superior removal of nitrogen with lower consumption of carbon. Overall, nitritation offers a practical pathway for integration with anaerobic ammonium oxidation for more sustainable and advanced nitrogen removal from real wastewater.

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甲酸盐调节亚硝酸盐氧化菌代谢在城市污水处理中的快速硝化作用。

通过可持续的方法改善城市污水中氮的去除对减轻全球氮污染至关重要。在本研究中，通过甲酸盐的添加，在8 天内快速实现了城市污水的硝化，亚硝酸盐积累率和总氮去除率分别稳定保持在76 %和93 %。相比之下，接受等量醋酸盐的对照组，尽管亚硝酸盐氧化细菌（NOB）的丰度较低，但未能实现硝化作用。此外，基于dna的稳定同位素探测、氧摄取速率和转录组分析表明，NOB优先利用氧或硝酸盐氧化甲酸盐可能是亚硝化的潜在机制。与其他碳源相比，甲酸酯的脱氮效果较好，且碳消耗较低。总的来说，硝化为与厌氧氨氧化相结合提供了一条实用的途径，可以更可持续地从实际废水中深度去除氮。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.