Bioresource Technology最新文献

{"title":"Effects of additives on shifting phosphorus to solid phase during Solid-Liquid separation of digestate in full-scale biogas plant","authors":"Naga Sai Tejaswi Uppuluri ,&nbsp;Xueling Ran ,&nbsp;Joachim Müller ,&nbsp;Jianbin Guo ,&nbsp;Hans Oechsner","doi":"10.1016/j.biortech.2024.131804","DOIUrl":"10.1016/j.biortech.2024.131804","url":null,"abstract":"<div><div>Phosphorus (P) is critical for plant growth, but global reserves are exhausting within 250–300 years, therefore enhancing phosphate recycling is crucial for the future. Biogas digestate, rich with nutrients is a promising resource for nutrient recovery. Conventional solid–liquid separation shifts approximately 35 % of the total P in the digestate to the solid phase. Separation trials with additive treatment using a screw press with a 0.75 <!--> <!-->mm sieve were performed at University of Hohenheim’s full-scale biogas plant. After 22 <!--> <!-->h, 67.41 % (kieserite treatment) and 52.35 % (straw<!--> <!-->flour treatment) of total P shifted to the solid phase. Treatment with kieserite enhanced P shift into the solid phase by forming non-labile fractions through a chemical bond between P and Mg²⁺ ions. Kieserite treatment for 22 h effectively increases the share of total P in the separated solid phase, it also ensures a sustainable nutrient supply and mitigates the risk of nutrient runoff.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131804"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthan gum production in Xanthomonas campestris is increased by favoring the biosynthesis of its monomers","authors":"Davi Benedito Oliveira ,&nbsp;Guilherme Engelberto Kundlastsch ,&nbsp;Richard Daniel Cruz ,&nbsp;Bruno Batista ,&nbsp;Marcelo Perencin de Arruda Ribeiro ,&nbsp;Maria Teresa Marques Novo-Mansur ,&nbsp;Adilson José da Silva","doi":"10.1016/j.biortech.2024.131808","DOIUrl":"10.1016/j.biortech.2024.131808","url":null,"abstract":"<div><div>Current efforts to improve xanthan gum (XG) production by <em>Xanthomonas</em> have focused on the growth medium, operating parameters, and downstream steps. However, a key aspect is the development of optimal strains. The present work aimed to investigate the formation of XG monomers, using kinetic and stoichiometric models to identify possible bottlenecks, and to engineer a recombinant strain to overcome such limitations. The <em>galU</em> and <em>ugd</em> genes involved in the<!--> <!-->biosynthesis of the UDP-glucose and UDP-glucuronic acid monomers were overexpressed in <em>Xanthomonas campestris</em> pv<em>. campestris.</em> The strains were cultivated in shake flasks and bioreactor. As predicted by <em>in silico</em> analysis, overexpression of the <em>ugd</em> gene resulted in a significant increase in gum synthesis, up to 50% higher volumetric productivity in the<!--> <!-->bioreactor. To a lesser extent, <em>galU</em> overexpression was also shown to improve product formation. These findings validated the hypothesis that metabolic engineering of the monomer biosynthesis can enhance XG production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131808"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From molasses to purified α-ketoglutarate with engineered Corynebacterium glutamicum","authors":"Lars Halle ,&nbsp;Daniela Höppner ,&nbsp;Marvin Doser ,&nbsp;Christian Brüsseler ,&nbsp;Jochem Gätgens ,&nbsp;Niclas Conen ,&nbsp;Andreas Jupke ,&nbsp;Jan Marienhagen ,&nbsp;Stephan Noack","doi":"10.1016/j.biortech.2024.131803","DOIUrl":"10.1016/j.biortech.2024.131803","url":null,"abstract":"<div><div>α-ketoglutarate (AKG) is a valuable dicarboxylic acid with multiple applications in the food, pharmaceutical, and chemical industries. Its chemical synthesis is associated with toxic by-products, low specificity, and high energy input. To create a more environmentally friendly and sustainable alternative, a microbial production process for AKG was developed. Four potential producer strains were generated by metabolic engineering of <em>Corynebacterium<!--> <!-->glutamicum</em> and characterized on defined glucose/sucrose media as well as molasses, a side stream from sugar beet processing. While strain <em>C.<!--> <!-->glutamicum</em> P_O6<em>-iolT</em>1<!--> <!-->Δ<em>gdh</em> Δ<em>gltB mscCG</em>’ Δ<em>odhA</em> was not able to grow on defined media it outperformed all predecessor variants on molasses. Successful scale-up into a fed-batch bioreactor process with molasses yielded 96.2<!--> <!-->g AKG with a conversion yield of 0.64<!--> <!-->g<!--> <!-->g⁻¹. Finally, downstream processing by liquid–liquid extraction with ethyl acetate enabled product purification with an extraction efficiency of 87 % and an AKG purity of &gt; 93 %.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131803"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering and evolution of Yarrowia lipolytica for producing lipids from lignocellulosic hydrolysates","authors":"Sangdo Yook ,&nbsp;Anshu Deewan ,&nbsp;Leah Ziolkowski ,&nbsp;Stephan Lane ,&nbsp;Payman Tohidifar ,&nbsp;Ming-Hsun Cheng ,&nbsp;Vijay Singh ,&nbsp;Matthew J. Stasiewicz ,&nbsp;Christopher V. Rao ,&nbsp;Yong-Su Jin","doi":"10.1016/j.biortech.2024.131806","DOIUrl":"10.1016/j.biortech.2024.131806","url":null,"abstract":"<div><div><em>Yarrowia lipolytica</em>, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the <em>Y. lipolytica</em> PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in <em>Y. lipolytica</em> and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131806"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Porphyridium purpureum and Porphyridium aerugineum as alternative resources for phycobiliprotein production","authors":"Liang Ji ,&nbsp;Chenni Zhao ,&nbsp;Yulong He ,&nbsp;Yuchen Yuan ,&nbsp;Zhiwei Hong ,&nbsp;Liyun Sun ,&nbsp;Jianhua Fan","doi":"10.1016/j.biortech.2024.131800","DOIUrl":"10.1016/j.biortech.2024.131800","url":null,"abstract":"<div><div>Microalgae not only fix carbon dioxide, but also represent a promising alternative resource for the production of proteins, lipids, and polysaccharides. This study employed two <em>Porphyridium</em> strains to compare their responses under different light qualities. <em>P. purpureum</em> up-regulated the content (up to 69.37 ± 0.92 mg/g DW) and proportion of phycoerythrin to enhance light absorption, which led to the accumulation of total soluble proteins, neutral lipids and exopolysaccharides under blue light. In contrast, <em>P. aerugineum</em> primarily improved the light energy utilization by increasing phycocyanin levels (up to 81.10 ± 0.60 mg/g DW), resulting in the degradation of neutral lipids and the accumulation of exopolysaccharides. Given the biomass, the highest yields of phycoerythrin (169.61 ± 2.90 mg/L) and phycocyanin (216.92 ± 1.90 mg/L) were achieved by <em>P. purpureum</em> and <em>P. aerugineum</em> cultured under white light, respectively. These findings indicate that <em>Porphyridium</em> can serve as a valuable resource for phycobiliprotein production, with biomolecules synthesis being tightly regulated by light quality.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131800"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-module engineering to guide the development of an efficient L-threonine-producing cell factory","authors":"Zhenqiang Zhao ,&nbsp;Jiajia You ,&nbsp;Xuanping Shi ,&nbsp;Mengmeng Cai ,&nbsp;Rongshuai Zhu ,&nbsp;Fengyu Yang ,&nbsp;Meijuan Xu ,&nbsp;Minglong Shao ,&nbsp;Rongzhen Zhang ,&nbsp;Youxi Zhao ,&nbsp;Zhiming Rao","doi":"10.1016/j.biortech.2024.131802","DOIUrl":"10.1016/j.biortech.2024.131802","url":null,"abstract":"<div><div>The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multi-module metabolic engineering was implemented to reprogram <em>Escherichia coli</em>, enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO₂ was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of L-threonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for <em>de novo</em> producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131802"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of substrate concentration on sulfamethoxazole wastewater treatment by osmotic microbial fuel cell: Insight into operational efficiency, dynamic changes of membrane fouling and microbial response.","authors":"Hengliang Zhang, Fei Xing, Liang Duan, Qiusheng Gao, Shilong Li, Yang Zhao","doi":"10.1016/j.biortech.2024.131805","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131805","url":null,"abstract":"<p><p>To solve the problems of antibiotic pollution, water resources and energy shortage, an osmotic microbial fuel cell (OsMFC) was adopted innovatively to treat antibiotic wastewater containing sulfamethoxazole (SMX), and achieved SMX removal, water production and electricity generation. Substrate concentration was a key factor affecting the performances of OsMFC, which was often ignored by researchers. This study explored the effect of substrate concentration on system performances, clarified the dynamic changes of membrane fouling under different substrate concentrations, and further revealed the response of microbial communities. The results showed that the stable removal efficiency of SMX exceeded 98.8 % due to the efficient interception of forward osmosis (FO) membrane. Compared with the 1.0 g/L NaAc system, the SMX degradation efficiency and maximum output voltage in the 2.0 g/L NaAc system were only increased by 3.9 % and 6.3 %, respectively. However, the initial water flux decreased by 30.1 % in the 7th cycle due to more serious FO membrane fouling. In addition, there were significant differences in the dynamic formation process of FO membrane fouling. Higher substrate concentration increased the relative abundance of Desulfobacterota and Geobacter. Functional prediction analysis showed that increasing substrate concentration promoted carbohydrate metabolism pathways and relative abundance of sulfur respiration functional groups, thereby improving COD and SMX removal rates. However, the biosynthesis of other secondary metabolites was significantly improved, resulting in increased contents of EPS and SMP, which aggravated membrane pollution. Overall, the system performed better when the substrate concentration was 1.0 g/L. This study would provide certain guidance for the performance optimization and membrane fouling mitigation of OsMFC, thereby promoting its practical application in antibiotic wastewater treatment.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131805"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust methane production from anaerobic digestion of maize silage: Feeding control using sliding mode control strategy with Anaerobic Digestion Model N°01","authors":"Abdelhani Chaabna,&nbsp;Samia Semcheddine","doi":"10.1016/j.biortech.2024.131749","DOIUrl":"10.1016/j.biortech.2024.131749","url":null,"abstract":"<div><div>This paper introduces a robust control approach based on the Sliding Mode Control (SMC) strategy for the Anaerobic Digestion (AD) process. The Anaerobic Digestion Model N°1 (ADM1), the most detailed and comprehensive model in this field, is used as a virtual AD plant. The proposed control scheme is derived from a reduced model. This control law utilizes the state variables of the reduced AM2HN model along with the stoichiometric and kinetic parameters. An interface block between the ADM1 and AM2HN state variables has been designed in a closed-loop configuration. The performance of SMC has been enhanced through the use of a low-pass filter, which completely eliminates chattering, providing smooth, chatter-free dilution rates while preserving the rapid convergence to target methane production (within less than 5 days) even in a noisy environment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131749"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainability assessment of blue hydrogen production through biomass gasification: A comparative analysis of thermal, solar, and wind energy sources.","authors":"Yousaf Ayub, Jianzhao Zhou, Tao Shi, Sara Toniolo, Jingzheng Ren","doi":"10.1016/j.biortech.2024.131798","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131798","url":null,"abstract":"<p><p>This study evaluated three blue hydrogen production processes - solar-grid powered, wind-powered, and thermal power grid (TPG) - considering the context of Hong Kong, SAR, China. A process sustainability analysis was performed based on energy, economic, and environmental (3E) factors. The energy efficiency analysis indicates that the TPG system is the most energy-efficient with 64% efficiency, followed by the wind power system at 63% and the hybrid solar-grid powered system at 60%. The economic analysis results indicate that the levelized cost of hydrogen (LCH) is 2.165 $/kg for the TPG system, 2.132 $/kg for the hybrid solar-grid powered system, and 2.060 $/kg for the wind power system. The environmental assessment suggests that wind powered are eco-friendly with a unit point total (µPt) of 1.11, compared to the solar-grid 1.50 µPt and TPG system's 1.70 µPt. Therefore, 3E analysis proposes wind powered process is more sustainable for blue H₂ production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131798"},"PeriodicalIF":9.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on the application of neural network model in microbial fermentation","authors":"Jia-Cong Huang ,&nbsp;Qi Guo ,&nbsp;Xu-Hong Li,&nbsp;Tian-Qiong Shi","doi":"10.1016/j.biortech.2024.131801","DOIUrl":"10.1016/j.biortech.2024.131801","url":null,"abstract":"<div><div>The development of high-performance strains and the continuous breakthrough of strain screening technology also pose challenges to downstream fermentation optimization and scale-up. Therefore, neural network models are utilized to optimize the fermentation process to meet the goals of boosting yield or lowering cost, with the use of artificial intelligence technology in conjunction with the peculiarities of the fermentation process. High-performance strains’ yield rise and fermentation process amplification will be sped up with the aid of neural network models. This paper offers a helpful review for anyone interested in state-of-the-art microbial fermentation processes, as it thoroughly reviews the application of neural network models in predicting fermentation yield, optimizing the fermentation process, and monitoring the fermentation process.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131801"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}