Bioresource Technology最新文献_第5页

Tailoring lignin surface properties for enhanced enzymatic hydrolysis: Comparative insights from pine and eucalyptus 剪裁木质素表面性质增强酶水解：比较见解从松树和桉树

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132972

Yoon-Jung Shin , Jae-Won Lee

{"title":"Tailoring lignin surface properties for enhanced enzymatic hydrolysis: Comparative insights from pine and eucalyptus","authors":"Yoon-Jung Shin , Jae-Won Lee","doi":"10.1016/j.biortech.2025.132972","DOIUrl":"10.1016/j.biortech.2025.132972","url":null,"abstract":"<div><div>Milled wood lignin (MWL) was isolated from pine and eucalyptus before and after hydrothermal treatment, and its effects on enzymatic hydrolysis were evaluated. Hydrothermal treatment primarily decomposed hemicellulose and induced structural changes in lignin for both species. With increasing treatment severity, the hydrophobicity and lignin surface area decreased, the zeta potential became more negative, aliphatic hydroxyl group content decreased, and β-O-4 linkages were cleaved. These changes influenced enzymatic hydrolysis, with the highest glucan to glucose conversion observed in pretreated eucalyptus (HT60). Correlation analysis indicated that enzymatic hydrolysis efficiency in pine was most strongly influenced by cellulose content, phenolic hydroxyl group in lignin, and zeta potential, while in eucalyptus, the key factors were cellulose content, aliphatic hydroxyl group content, and zeta potential. The role of lignin hydroxyl group in enzyme adsorption was further examined through MWL acetylation, which demonstrated that acetylated MWL exhibited reduced enzyme adsorption compared to unmodified MWL.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132972"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Augmented methanogenic performance in straw-manure co-digestion via micro/nano bubble-enhanced syntrophic metabolism 微/纳米气泡增强合成代谢提高秸秆粪共消化产甲烷性能

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132955

Yanning Hou , Chao Liu , Jianhong Wei , Wenyan Zhao , Binghua Yan

{"title":"Augmented methanogenic performance in straw-manure co-digestion via micro/nano bubble-enhanced syntrophic metabolism","authors":"Yanning Hou , Chao Liu , Jianhong Wei , Wenyan Zhao , Binghua Yan","doi":"10.1016/j.biortech.2025.132955","DOIUrl":"10.1016/j.biortech.2025.132955","url":null,"abstract":"<div><div>This study aimed to investigate the enhancement of methane production by comparing air-nanobubble water (air-NBW) and conventional micro-bubble aeration during the anaerobic co-digestion (AcoD) of rice straw and pig manure. Remarkably, the addition of air-NBW (T3-NBW), as the most effective gas supplementation strategy, resulted in the highest cumulative methane yield of 489.49 mL/g·VS with a minimal lag phase of 0.57 days, representing a 59.80 % increase over the control (306.49 mL/g·VS, <em>p</em> < 0.001). This finding aligns with the kinetic fitting data (477.85 ± 5.84 mL/g·VS), wherein the maximum methane production rate for the T3-NBW was 41.89 mL/g∙VS·d<sup>−1</sup>. Mechanistic insights revealed that air-NBW dramatically elevated the activity of β-glucosidase (hydrolysis) and coenzyme F<sub>420</sub> (methanogenesis) by 82.72% and 133.8 %, respectively, compared to the control. These results suggested that the efficient conversion of intermediate products played a critical role in enhancing overall methane yield. Microbial community analysis identified acetotrophic methanogenesis as the dominant pathway, with <em>Methanosaeta</em> abundance reaching 91.66 % in NBW-amended treatments. Additionally, the co-occurrence network analysis showed that the air-NBW system promoted microbial interaction and stabilized the ecological network. Crucially, the NBW treatment exhibited the lowest expression of energy-dependent antioxidant enzyme genes (e.g., superoxide reductase, <em>SOR</em>), suggesting reduced oxidative stress and enhanced energy allocation toward microbial growth and methanogenic activity. By enhancing oxygen dispersion and microbial intracellular redox balance, NBW technology provides a promising approach for improving methane recovery in sustainable waste-to-energy systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132955"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monte-Carlo ray tracing simulation of view factors for diffuse solar radiation determination in cylindrical photobioreactor arrays for microalgae cultivation 微藻培养圆柱形光生物反应器阵列中散射太阳辐射观测因子的蒙特卡罗射线追踪模拟

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132813

Yihan Zheng , Lifeng Li , Shengkai Dai , Donghang Liu , Hao Zheng , Song Yang , Yanming Guo , Muhammad Rafique , Bo Wang , Chi-Hwa Wang , Yong Shuai

{"title":"Monte-Carlo ray tracing simulation of view factors for diffuse solar radiation determination in cylindrical photobioreactor arrays for microalgae cultivation","authors":"Yihan Zheng , Lifeng Li , Shengkai Dai , Donghang Liu , Hao Zheng , Song Yang , Yanming Guo , Muhammad Rafique , Bo Wang , Chi-Hwa Wang , Yong Shuai","doi":"10.1016/j.biortech.2025.132813","DOIUrl":"10.1016/j.biortech.2025.132813","url":null,"abstract":"<div><div>In this study, a Monte Carlo ray tracing (MCRT) model was developed to calculate view factors between cylindrical photobioreactor (PBR) sidewalls and sky/ground surfaces in outdoor array configurations. The effects of PBR dimension, inter-PBR spacing and array layout, namely square, staggered and biomimetic layouts, were analyzed. Results showed that view factors are mainly governed by mutual shading effects. In staggered arrays, centrally positioned PBRs exhibited significantly lower view factors compared to peripheral units, with reductions reaching up to 97%. Reducing inter-PBR spacing from 1.26m to 0.56m in staggered configurations resulted in a 46% decrease in mean view factor (from 0.301 to 0.163). Comparative analysis revealed average view factors of 0.2410, 0.2416 and 0.2259 for square, staggered, and biomimetic layouts, respectively. This work establishes a methodology for quantifying diffuse radiation received by PBR arrays which is indispensable for PBR multiphysics simulations.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132813"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced autotrophic denitrification under salinity stress by bioaugmentation of Sulfurimonas: performance evaluation, microbial community and response mechanisms 盐胁迫下硫单胞菌生物强化自养反硝化：性能评价、微生物群落及响应机制

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132971

Liang Cui , Shasha Wang , Xiaxing Cao , Shicheng Chen , Jiannan Wang , Zhen Chen , Lijing Jiang , Xuezhe Wen , Wanpeng Wang , Qianqian Wang , Zhou Zhou , Suping Yang , Xuesong Yi , Yong Wang , Zongze Shao

{"title":"Enhanced autotrophic denitrification under salinity stress by bioaugmentation of Sulfurimonas: performance evaluation, microbial community and response mechanisms","authors":"Liang Cui , Shasha Wang , Xiaxing Cao , Shicheng Chen , Jiannan Wang , Zhen Chen , Lijing Jiang , Xuezhe Wen , Wanpeng Wang , Qianqian Wang , Zhou Zhou , Suping Yang , Xuesong Yi , Yong Wang , Zongze Shao","doi":"10.1016/j.biortech.2025.132971","DOIUrl":"10.1016/j.biortech.2025.132971","url":null,"abstract":"<div><div>Sulfur autotrophic denitrification (SADN) is a promising nitrogen removal process for industrial wastewater treatment, but elevated salinity is a common inhibitory factor that affects SADN performance. In this study, the sequencing batch biofilm reactor (SBBR) was enhanced with isolated salt-tolerant <em>Sulfurimonas</em> strains. Results showed that bioaugmentation improved the reactor’s denitrification efficiency from 70.21 % to 98.79 %. As salinity increased from 1 % to 4 %, nitrogen removal efficiency experienced short fluctuations and quickly stabilized above 98.50 %. The abundance of <em>Sulfurimonas</em> increased from 13.06 % to 26.14 % with increasing salinity, becoming the most dominant taxon at high salinity. This genus maintained the highest abundance during sulfur disproportionation within the entire reactor. Network analysis indicated that <em>Sulfurimonas</em> primarily exhibited competitive relationships with other microbial groups. Additionally, there was a notable increase in gene abundance associated with denitrification and dissimilatory nitrate reduction. Overall, this study offers an efficient alternative method for nitrate removal in carbon-limited, high-salinity wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132971"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced dye treatment and bioelectricity generation in microbial fuel cells: the role of magnetite-inoculated anode and cosubstrates 微生物燃料电池中强化染料处理和生物发电：磁铁矿接种阳极和辅基质的作用

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132965

Minh Duc Tran , Thu Huong Nguyen , Takahiro Watari , Keiichi Kubota , Tomoki Setoguchi , Masashi Hatamoto , Takashi Yamaguchi

{"title":"Enhanced dye treatment and bioelectricity generation in microbial fuel cells: the role of magnetite-inoculated anode and cosubstrates","authors":"Minh Duc Tran , Thu Huong Nguyen , Takahiro Watari , Keiichi Kubota , Tomoki Setoguchi , Masashi Hatamoto , Takashi Yamaguchi","doi":"10.1016/j.biortech.2025.132965","DOIUrl":"10.1016/j.biortech.2025.132965","url":null,"abstract":"<div><div>This study investigated the influence of cosubstrate complexity and magnetite-inoculated anodes on microbial fuel cells (MFCs) treating synthetic dyeing wastewater (500 mgCOD/L, 50 mg/L Reactive Black 5). Two MFCs types were constructed: one with magnetite pre-inoculated anodes (MFCM) and another with sludge-only inoculated anodes (MFCS). Both MFCs were operated with either starch or ethanol as the cosubstrate, and in both cases, MFCM consistently outperformed MFCS. For instance, with starch, MFCM achieved 99 % decolorization and a peak voltage of 380.74 mV within 30 hours, compared to 89 % and negligible, unstable voltage in MFCS. A 1500 Ω resistance was optimal for decolorization and electricity generation in MFCM. Microbial analysis revealed that magnetite pre-inoculation enriched electroactive genera, enhanced diversity and extracellular electron transfer, and stabilized microbial networks across cosubstrates. These findings highlight the potential of magnetite pre-inoculated anodes as a novel practical strategy for early anode biofilm control, with significant implications for scalable MFCs applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132965"},"PeriodicalIF":9.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of anaerobic digestion of cattle manure to continuous temperature transitions: Process performance, microbial succession, key enzymes, and metabolic pathways 牛粪厌氧消化对持续温度变化的响应：过程性能、微生物演替、关键酶和代谢途径

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132953

Chenyang Zhu , Heran Wang , Rufei Liu , Hairong Yuan , Xiujin Li

{"title":"Response of anaerobic digestion of cattle manure to continuous temperature transitions: Process performance, microbial succession, key enzymes, and metabolic pathways","authors":"Chenyang Zhu , Heran Wang , Rufei Liu , Hairong Yuan , Xiujin Li","doi":"10.1016/j.biortech.2025.132953","DOIUrl":"10.1016/j.biortech.2025.132953","url":null,"abstract":"<div><div>This study investigated the effect of continuous temperature transitions on the anaerobic digestion (AD) performance of cattle manure (CM) and microbial response mechanisms. Experiments were conducted in a completely-stirred tank reactor (CSTR) for over 390 days with a fine 3 °C gradient from 35 °C to 65 °C. The results showed that AD achieved optimal performance at R47 (47 °C). Microbial analysis indicated that 47 °C was thecritical temperaturefor the transition from mesophilic to thermophilic communities. Metagenomic analysis revealed that, compared with R38 (38 °C) and R53 (53 °C), R47 enhanced the hydrolysis efficiency of refractory components by upregulating key enzyme expression in glycolysis and pyruvate metabolism. At R47,elevatedexpression of key enzymes inboth acetate/butyrate metabolic pathways and methylotrophic methanogenesis pathway contributed to the highest methane yield. The optimal temperature determined for CM provides important parameters for practical AD operations.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132953"},"PeriodicalIF":9.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cosubstrates in azo dye decolorization: From conventional anaerobic systems to microbial fuel cells 偶氮染料脱色中的共底物：从传统厌氧系统到微生物燃料电池

IF 11.4 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132962

Thu Huong Nguyen, Nguyen Anh-Vu, Thi Thu Huong Nguyen, Taira Hidaka, Taku Fujiwara, Takahiro Watari, Takashi Yamaguchi

{"title":"Cosubstrates in azo dye decolorization: From conventional anaerobic systems to microbial fuel cells","authors":"Thu Huong Nguyen, Nguyen Anh-Vu, Thi Thu Huong Nguyen, Taira Hidaka, Taku Fujiwara, Takahiro Watari, Takashi Yamaguchi","doi":"10.1016/j.biortech.2025.132962","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132962","url":null,"abstract":"Cosubstrates play a pivotal role in anaerobic azo dye treatment, including both conventional and emerging microbial fuel cells (MFCs) systems, but their mechanistic roles are often overlooked. Compounding this issue is the persistent shortage of biodegradable cosubstrates in real textile wastewater, emphasizing the need for strategic evaluation and optimization. This review systematically analyzes how cosubstrate types and concentrations affect decolorization performance in both systems, based on a wide range of previous studies. Beyond identifying effective cosubstrates and optimal dosage ranges, this review explores the biochemical and microbiological mechanisms underlying these differences. Furthermore, the potential of waste-derived cosubstrates is discussed as a sustainable strategy to improve treatment efficiency and address the shortage of carbon donors Cosubstrates are more than just auxiliary carbon sources; rather, they serve as central regulators of microbial coordination, enzymatic activity, and electron flow. Recognizing their multifaceted roles is key to optimizing future treatment strategies.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"29 1","pages":"132962"},"PeriodicalIF":11.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The recovery mechanism of granular sludge fragmentation and re-granulation caused by long-term high-concentration organic matter erosion in the SAD process: performance, sludge evolution, and metagenomic sequencing SAD过程中长期高浓度有机物侵蚀引起的颗粒污泥破碎和再造粒的恢复机制：性能、污泥演化和宏基因组测序

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132966

Peng-fei Yu, Ding Wang, Yu-bo Fu, Xing-guan Ma, Hao Zheng, Li-yao Han, Ao Wang, Dian-lin Jiang, Hai-wen Sun, Xing Jin

{"title":"The recovery mechanism of granular sludge fragmentation and re-granulation caused by long-term high-concentration organic matter erosion in the SAD process: performance, sludge evolution, and metagenomic sequencing","authors":"Peng-fei Yu, Ding Wang, Yu-bo Fu, Xing-guan Ma, Hao Zheng, Li-yao Han, Ao Wang, Dian-lin Jiang, Hai-wen Sun, Xing Jin","doi":"10.1016/j.biortech.2025.132966","DOIUrl":"10.1016/j.biortech.2025.132966","url":null,"abstract":"<div><div>The Simultaneous Anammox and Denitrification (SAD) process effectively removes organic carbon sources, the impact of high-concentration carbon sources on the SAD process remains unclear. This study investigated the performance, sludge characteristics, microbial community correlations, and metagenomic sequencing of the SAD system under conditions of excessive organic matter exposure. The results showed that the organic matter metabolism ability of SAD granular sludge increased from 90.16 ± 1.16 % to 95.2 ± 2.3 %. The Mantel test revealed that Anaerobic Ammonium Oxidation Bacteria (AnAOB) (<em>Candidatus</em>_Kuenenia) were positively correlated with VSS/SS and instability coefficient, while Denitrifying Bacteria (DNB) (<em>Truepera</em>, <em>Ottowia</em>, <em>Deniratisoma</em>, <em>Arenimonas</em>) were negatively correlated with wet density, settling velocity, granule size, protein/polysaccharides (PN/PS) ratio, and the complete coefficient. Following the recovery of the SAD system, the bacterial community correlations increased, and the stability and mechanical strength of the granular sludge were enhanced. Metagenomic sequencing showed that a decrease of Quorum sensing (QS) and the increase of c-di-GMP levels led to up-regulation of exopolysaccharide and extracellular protein expression, resulting in the disintegration of SAD granular sludge. As the sludge aggregated, recombined, and re-granulated, the system up-regulated the expression of the hzs gene in AnAOB nitrogen metabolism via c-di-GMP and QS signals. It also up-regulated the expression of genes such as Dissimilatory Nitrate Reduction to Ammonium (DNRA) and potential denitrification pathways, enhancing the metabolism of AnAOB and symbiotic bacteria.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132966"},"PeriodicalIF":9.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic redundancy of catechol 1,2-dioxygenase conferred the ability of Rhodococcus opacus PD630 for efficient catecholic compounds degradation 儿茶酚1,2-双加氧酶的遗传冗余赋予了不透明红球菌PD630有效降解儿茶酚化合物的能力

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132964

Xinjie Chen , Zhenzhen Cai , Siqi Jiang , Mingyuan Cheng , Xiaoyu Zhang , Fuying Ma , Hongbo Yu , Yuzhou Wu , Fangrui Zhong , Ning Xiao , Shangxian Xie

{"title":"Genetic redundancy of catechol 1,2-dioxygenase conferred the ability of Rhodococcus opacus PD630 for efficient catecholic compounds degradation","authors":"Xinjie Chen , Zhenzhen Cai , Siqi Jiang , Mingyuan Cheng , Xiaoyu Zhang , Fuying Ma , Hongbo Yu , Yuzhou Wu , Fangrui Zhong , Ning Xiao , Shangxian Xie","doi":"10.1016/j.biortech.2025.132964","DOIUrl":"10.1016/j.biortech.2025.132964","url":null,"abstract":"<div><div>Lignin, a bountiful natural resource, presents challenges in degradation and conversion due to its complex structure. Microorganisms have evolved a “biofunnel” pathway in nature that offers novel insights into lignin valorization. Among the key intermediates in lignin metabolism is catechol, predominantly metabolized by catechol 1,2-dioxygenase (CatA) in these organisms. Understanding the catechol degradation mechanism is pivotal for efficient lignin utilization. <em>Rhodococcus opacus</em> PD630 emerges as a promising host for lignin bioconversion, yet the gene redundancy of catechol 1,2-dioxygenase genes (<em>catA</em>) in this strain is not well understood, hindering a comprehensive grasp of its aromatic compound degradation pathway. This study explores the regulatory mechanisms governing the expression of <em>catA</em> gene duplicates, LPD06568 <em>(catA1)</em> and LPD06742 <em>(catA2)</em>, in <em>R. opacus</em> PD630. We compared the functions of the enzymes they encode and discovered that <em>catA2</em> is responsible for the degradation of excess extracellular catechol in <em>R. opacus</em> PD630. More interestingly, our results also showed that both of the CatAs of <em>R. opacus</em> PD630 could catalyze various catecholic compounds, including those with large side chain groups. Furthermore, <em>catA1</em> was heterologously expressed in <em>Streptomyces coelicolor</em> M145 and achieved a marked cis, <em>cis</em>-muconic acid production (1.97 g/L) from catechol.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132964"},"PeriodicalIF":9.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulating the Carbon-to-Nitrogen ratio for enhanced reconstruction efficiency in Litopenaeus vannamei aquaculture systems 调节碳氮比提高凡纳滨对虾养殖系统改造效率

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-10 DOI: 10.1016/j.biortech.2025.132961

Lei Cao , Shuonan Ma , Shuaicheng Xiang , Yantao Li , Xuezhi Zhang , Zhuoyi Zhu , Xiaojun Yan , Roger Ruan , Pengfei Cheng

{"title":"Regulating the Carbon-to-Nitrogen ratio for enhanced reconstruction efficiency in Litopenaeus vannamei aquaculture systems","authors":"Lei Cao , Shuonan Ma , Shuaicheng Xiang , Yantao Li , Xuezhi Zhang , Zhuoyi Zhu , Xiaojun Yan , Roger Ruan , Pengfei Cheng","doi":"10.1016/j.biortech.2025.132961","DOIUrl":"10.1016/j.biortech.2025.132961","url":null,"abstract":"<div><div>Intensive shrimp farming faces challenges of water quality deterioration and excessive chemical usage. This study utilized glucose supplementation to regulate carbon-to-nitrogen ratios (C/N): C/N = 5 (control), 10, 15, and 20, comprehensively evaluating impacts on water quality, shrimp growth performance, and product quality. Results revealed that C/N = 20 significantly improved the aquatic environment, achieving reductions of 57.43 % in nitrite-N (NO<sub>2</sub><sup>−</sup>-N), 59.86 % in nitrate-N (NO<sub>3</sub><sup>−</sup>-N), 67.79 % in total nitrogen (TN), 67.02 % in total phosphorus (TP), and 98.37 % in ammonia-N (NH<sub>4</sub><sup>+</sup>-N). The C/N = 15 group demonstrated optimal growth performance, with body length, body weight, and specific growth rate (SGR) increasing by 15.91 %, 31.89 %, and 33.21 % respectively versus controls, while simultaneously enhancing muscle nutritional composition, amino acid profile, and textural properties. Organic carbon amendment promoted heterotrophic microbial dominance, forming bioflocs that improved feed utilization efficiency. By integrating microbial ecological regulation with nutritional management, this study establishes a scientifically validated, eco-friendly aquaculture strategy that reduces dependence on chemical interventions, offering dual theoretical and practical significance for sustainable industry development.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132961"},"PeriodicalIF":9.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0