Bioresource Technology最新文献_第5页

Insights into long-term effects of low-dose cerium on partial-nitrification process: Accelerating nitrite supply by endogenous heterotrophic nitrification partial denitrification 低剂量铈对部分硝化过程的长期影响：内源性异养硝化部分反硝化加速亚硝酸盐供应

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-30 DOI: 10.1016/j.biortech.2025.132604

Hao Su , Bingyan Dong , Longwen Xiao , Dachao Zhang

{"title":"Insights into long-term effects of low-dose cerium on partial-nitrification process: Accelerating nitrite supply by endogenous heterotrophic nitrification partial denitrification","authors":"Hao Su , Bingyan Dong , Longwen Xiao , Dachao Zhang","doi":"10.1016/j.biortech.2025.132604","DOIUrl":"10.1016/j.biortech.2025.132604","url":null,"abstract":"<div><div>Cerium (Ce(III)) is ubiquitously quantified in many rivers (up to 4.48 mg/L), especially in nitrogen-rich streams affected by rare earth mining. This study investigated effects and mechanisms for long-term (123 d) low-dose (0.5–5.0 mg/L) Ce(III) on partial-nitrification (PN) process. In this study, Ach1 and PrpE were upregulated by 1.0 mg/L Ce(III), which increased the proportion of acetate in metabolic products and enriched <em>Pseudofulvimonas</em>. Moreover, PN/endogenous nitrification partial denitrification (PN/ENPD) process established via the synergy of <em>Pseudofulvimonas</em> and <em>Nitrosomonas</em>, which increased specific nitrite production rate (60 %) and decreased N<sub>2</sub>O (95 %). Furthermore, the “biotic/abiotic synergistic detoxification mechanism” activated when Ce(III) concentration reached 1.0 mg/L, which increased abundance of <em>Nitrosomonas</em>, performance, and secretion of biomolecules. Finally, PN/ENPD process collapsed when Ce(III) concentration reached 5.0 mg/L due to Ce(III) accumulated toxicity. Overall, this study advances the understanding of long-term risks of low-dose Ce(III) to PN process and provides novel insights into accelerating nitrite supply.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132604"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe3O4 nanoparticles promote methanogenesis in propionate acclimated system 纳米Fe3O4促进丙酸驯化体系的甲烷生成

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-30 DOI: 10.1016/j.biortech.2025.132608

Fangzhou Wang , Ge Song , Mou Zhang , Shunan Zhao , Tuo Wang , Kai Zhao , Xin Wang , Ruiping Liu

{"title":"Fe3O4 nanoparticles promote methanogenesis in propionate acclimated system","authors":"Fangzhou Wang , Ge Song , Mou Zhang , Shunan Zhao , Tuo Wang , Kai Zhao , Xin Wang , Ruiping Liu","doi":"10.1016/j.biortech.2025.132608","DOIUrl":"10.1016/j.biortech.2025.132608","url":null,"abstract":"<div><div>Propionate accumulation is common in anaerobic digestion systems, while Fe<sub>3</sub>O<sub>4</sub> nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) show potential in steering direct interspecies electron transfer (DIET) to facilitate methanogenesis from propionate. However, the effect of Fe<sub>3</sub>O<sub>4</sub> NPs in stable microbial communities remains unclear. This study demonstrated that 1.0 g/L Fe<sub>3</sub>O<sub>4</sub> NPs enhanced propionate degradation and methane production by 220 % and 55 % of the propionate-acclimated microbial consortia post-shock loading, as evidenced by batch-test. High propionate concentrations suppressed <em>Geobacter</em>, yet Fe<sub>3</sub>O<sub>4</sub> NPs enabled electron syntrophy between alternative DIET-participants (<em>Arcobacter</em>, <em>Syntrophobacter</em>) and methanogens (<em>Methanothrix</em>, <em>Methanobacterium</em>) serving as electron conduits. Network analysis further revealed that Fe<sub>3</sub>O<sub>4</sub> NPs activated interactions among potential electroactive microbes, highlighting the potentially ubiquitous presence of electron syntrophy. Even in propionate-stressed microbial communities, such mutualism may be rapidly activated by Fe<sub>3</sub>O<sub>4</sub> NPs, offering a practical solution for mitigating propionate accumulation and enhance digester performance.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132608"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated sludge granulation of novel complete ammonium and nitrate removal via denitratation anammox over nitrite process at elevated loading rates 在高负荷速率下，通过亚硝酸盐反硝化厌氧氨氧化工艺加速新型完全去除铵硝的污泥造粒

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-30 DOI: 10.1016/j.biortech.2025.132610

Ziyi Cheng , Jinyan Wang , Xinping Liu , Shenbin Cao

{"title":"Accelerated sludge granulation of novel complete ammonium and nitrate removal via denitratation anammox over nitrite process at elevated loading rates","authors":"Ziyi Cheng , Jinyan Wang , Xinping Liu , Shenbin Cao","doi":"10.1016/j.biortech.2025.132610","DOIUrl":"10.1016/j.biortech.2025.132610","url":null,"abstract":"<div><div>The Complete Ammonium and Nitrate Removal via Denitratation Anammox Over Nitrite (CANDAN) process was evaluated for rapid sludge granulation in a lab-scale sequencing batch reactor. Over 119 days under increasing nitrogen loading rates (NLRs), the system finally achieved average 89.2 % total nitrogen removal at 1.93 kg N/m<sup>3</sup>/d NLR, with sludge particle sizes increasing from 215.6 μm to 924.5 μm. Higher NLRs significantly increased extracellular polymeric substances, especially hydrophobic proteins, enhancing sludge hydrophobicity and aggregation. Metagenomic analysis identified <em>Candidatus Brocadia</em> and <em>Thauera</em> as predominant and key microbial genera for nitrogen removal. Furthermore, the upregulation of carbon metabolism under heightened NLRs facilitated the synthesis of hydrophobic amino acids, promoting sludge granulation. These findings demonstrate NLR-driven granulation mechanisms, highlight optimizing NLR as key for accelerating granulation, providing insights to improve start-up and operational efficiency of CANDAN systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132610"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into microalgal-bacterial immobilization systems for wastewater treatment: mechanisms, enhancement strategies, and application prospects 微藻-细菌固定化系统在废水处理中的新进展：机制、增强策略和应用前景

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-30 DOI: 10.1016/j.biortech.2025.132609

Lili Yang , Xin Sun , Hongwei Li , Ran Hao , Fengling Liu

{"title":"New insights into microalgal-bacterial immobilization systems for wastewater treatment: mechanisms, enhancement strategies, and application prospects","authors":"Lili Yang , Xin Sun , Hongwei Li , Ran Hao , Fengling Liu","doi":"10.1016/j.biortech.2025.132609","DOIUrl":"10.1016/j.biortech.2025.132609","url":null,"abstract":"<div><div>The wastewater treatment based on the symbiosis of microalgae and bacteria has attracted increasing attention for its excellent pollutant removal efficiency, energy savings, and resource recovery. Among them, the microalgae-bacteria immobilization (MABI) system stands out by enhancing the electron transfer efficiency through carrier domain confinement, thereby overcoming bottlenecks of low light energy utilization and challenging biomass recycling. MABI is considered a key breakthrough for advancing engineering applications. However, a comprehensive exploration of MABI systems remains lacking. This review systematically summarizes the latest advancements, covering major immobilization techniques and the intrinsic mechanisms underlying microalgae-bacteria interactions and electron transport. Additionally, it explores enhancement strategies aimed at balancing microbial light energy allocation, optimizing nutrient supply, and constructing complementary ecological niches. The advantages and application prospects of MABI systems are highlighted. The review contributes to structuring the knowledge framework of MABI research and identifies critical gaps for future investigation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132609"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering of electron transfer and microbial community of electrogenic oxygen reducing biofilms to sulfamethoxazole stress 电致氧生物膜在磺胺甲恶唑胁迫下的电子转移和微生物群落解析

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132597

Qing Jiang , Xing Dong , Yang Liu , Xiaoyu Zhou , Guomeng Sun , Ke Shi , Yanlu Qiao , Hao Jiang , Yujie Feng

{"title":"Deciphering of electron transfer and microbial community of electrogenic oxygen reducing biofilms to sulfamethoxazole stress","authors":"Qing Jiang , Xing Dong , Yang Liu , Xiaoyu Zhou , Guomeng Sun , Ke Shi , Yanlu Qiao , Hao Jiang , Yujie Feng","doi":"10.1016/j.biortech.2025.132597","DOIUrl":"10.1016/j.biortech.2025.132597","url":null,"abstract":"<div><div>This study first evaluated the sulfamethoxazole (SMX) effects on oxygen-reducing biocathodes in microbial fuel cells (MFCs). Low SMX (0.5 mg L<sup>–1</sup>) enhanced current density by 20 % via increased direct electron transfer and lower charge transfer resistance. High SMX (10–30 mg L<sup>–1</sup>) suppressed electrochemical performance. SMX preferentially bound protein-like EPS components over fulvic-like fractions, inducing sequential structural changes (1054 > 970 > 3464 > 2921 > 1643 > 1350 cm<sup>−1</sup>). SMX exposure reshaped microbial communities, enriching antibiotic-resistant genera (<em>Truepera</em>, <em>Nitrospira</em>, <em>Brevundimonas</em>, etc.). Network analysis revealed low SMX enhanced community complexity/stability, while high doses simplified biofilm structure. Functional genes for electron transfer, carbon metabolism and oxidative phosphorylation increased at 0.5 mg L<sup>–1</sup> SMX but decreased under high concentrations. Overall, this study elucidates the dual role of SMX in modulating oxygen-reducing biofilm composition, function, and capability, laying the groundwork for optimized application of MFC in treating SMX-contaminated wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132597"},"PeriodicalIF":9.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of new thermostable MtLPMO9V in synergism with cellulases for efficient lignocellulosic hydrolysis 新型耐热MtLPMO9V与纤维素酶协同作用高效水解木质纤维素的构建

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132594

Weimeng Li , Hang Yuan , Yun Hu , Shaohua Dou , Ezhen Zhang , Qun Wu , Fubao Sun

{"title":"Construction of new thermostable MtLPMO9V in synergism with cellulases for efficient lignocellulosic hydrolysis","authors":"Weimeng Li , Hang Yuan , Yun Hu , Shaohua Dou , Ezhen Zhang , Qun Wu , Fubao Sun","doi":"10.1016/j.biortech.2025.132594","DOIUrl":"10.1016/j.biortech.2025.132594","url":null,"abstract":"<div><div>Lytic polysaccharide monooxygenases (LPMOs) can promote cellulose hydrolysis by disrupting its crystalline zone. This study focused on an uncharacterized thermophilic <em>Myceliophthora thermophila</em> LPMO (<em>Mt</em>LPMO9V) in synergism with cellulases for efficient ligocellulosic hydrolysis. After <em>Mt</em>LPMO9V was successfully expressed in <em>P. pastoris</em> GS115, the oxidative depolymerization of it was characterized by HPLC, HPAEC-PAD, and MALDI-TOF MS, indicating C4 oxidative cleavage activity. With combination of computer-aided design and MD simulation, <em>Mt</em>LPMO9V was improved for a higher catalytic activity and thermostability by introduction of disulfide bonds, followed by point mutation. The mutant, A170C/A175C/Q120Y (M3), exhibited a remarkable enzymatic activity, increasing by 88 % as compared to the wild-type <em>Mt</em>LPMO9V (WT), in which the catalytic efficiency (<em>k<sub>cat</sub></em>/<em>K<sub>m</sub></em>) was roughly 1.90 folds that of the WT. The M3 demonstrated broad applicability, not only showing synergism with the thermostable endoglucanase <em>Dt</em>CelA for efficient high-temperature saccharification of cellulosic substrates, but also enhancing the saccharification of lignocellulosic substrates when combined with the commercial cellulase blend Celluclast 1.5L, where LPMO accounts for only 2–4 % of the cellulase mixture. This study provides valuable insights into engineering of new extreme LPMOs and also exhibits their potential applicability in development of cellulase-mediated lignocellulosic biorefinery industry.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132594"},"PeriodicalIF":9.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing simultaneous nitrogen and phosphorus removal from municipal wastewater using micron zeolite powder carrier and hydrocyclone separator: Microbial distribution and correlation analysis 微沸石粉体载体与水力旋流分离器强化城市污水同时脱氮除磷：微生物分布及相关性分析

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132598

Hong Wang , Shiyu Liu , Yanzeng Li , Xinyu Li , Lei Li , Shijie Yuan , Xiaohu Dai

{"title":"Enhancing simultaneous nitrogen and phosphorus removal from municipal wastewater using micron zeolite powder carrier and hydrocyclone separator: Microbial distribution and correlation analysis","authors":"Hong Wang , Shiyu Liu , Yanzeng Li , Xinyu Li , Lei Li , Shijie Yuan , Xiaohu Dai","doi":"10.1016/j.biortech.2025.132598","DOIUrl":"10.1016/j.biortech.2025.132598","url":null,"abstract":"<div><div>This study developed a novel wastewater treatment process for efficient nitrogen and phosphorus removal using micron zeolite powder carriers and hydrocyclone separator. Under anaerobic/intermittent aeration, the total nitrogen and phosphorus removal efficiencies reached 85.2 ± 1.9 % and 78.9 ± 3.4 %, respectively, significantly outperforming conventional activated sludge system. High specific surface area and porosity of zeolite powder facilitated microbial aggregation and biofilm formation, resulting in an average sludge size of 125.3 ± 5.3 μm. The combination of powder carriers and hydrocyclone separators resulted in the differentiated distribution of functional microorganisms. Denitrifying bacteria, such as norank_Comamonadaceae (4.34 %), norank_AKYH767 (1.90 %), and Candidatus_Microthrix (2.61 %), were enriched in biofilm, while nitrifying bacteria and polyphosphate-accumulating organisms predominated in floc. Functional gene abundance related to denitrification and phosphorus removal was significantly upregulated. Correlation network analysis revealed enhanced microbial cooperation, improving the functionality and stability of community. This study offers the potential pathway for efficient nitrogen and phosphorus removal from municipal wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132598"},"PeriodicalIF":9.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of docosahexaenoic acid from corncob residue wastes through integrated whole-cell lignocellulosic saccharification and lipid fermentation by Aurantiochytrium 全细胞木质纤维素糖化和金氧化钇脂质发酵玉米芯渣生产二十二碳六烯酸的研究

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132606

Xiaojin Song , Weijian Wan , Yuedong Zhang , Guang Yu , Bin Li , Qiu Cui , Ya-Jun Liu , Yingang Feng

引用次数: 0

Enhancing mixed-species microalgal biofilms for wastewater treatment: Design, construction, evaluation and optimisation 强化混合微藻生物膜用于废水处理：设计、建造、评价和优化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132600

Zhinan Liang, Hang Zhong, Quanyu Zhao

{"title":"Enhancing mixed-species microalgal biofilms for wastewater treatment: Design, construction, evaluation and optimisation","authors":"Zhinan Liang, Hang Zhong, Quanyu Zhao","doi":"10.1016/j.biortech.2025.132600","DOIUrl":"10.1016/j.biortech.2025.132600","url":null,"abstract":"<div><div>Biofilm-based cultivation of microalgae is a powerful method for wastewater treatment with low harvesting costs, water and energy consumption. This article provides a detailed summary of the design, construction, evaluation, and optimisation (DCEO) of mixed-species biofilms including algal and bacteria, and discusses their relevant applications in the treatment of industrial and agricultural wastewater and new pollutants. Finally, it presents the problems faced by mixed-species microalgal biofilms, along with solutions. DCEO is a typical synthetic biology concept, in which design and construction are bottom-up, and evaluation and optimisation are top-down approaches. Detailed knowledge of the metabolic pathways and the regulation of microalgae and other microorganisms is helpful for designing mixed-species biofilms. Three dimensional bioprinting is a powerful tool for constructing structured biofilms. Further analysis after evaluation is beneficial to optimise such biofilms. This review provides a new insight into using DCEO to enhance mixed-species biofilms for wastewater treatment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132600"},"PeriodicalIF":9.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of aldehyde:ferredoxin oxidoreductase-encoding genes in Clostridium ljungdahlii results in changes in product spectrum with various carbon sources ljungdahli梭菌醛氧还蛋白氧化还原酶编码基因的缺失导致了不同碳源下产物谱的变化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-04-28 DOI: 10.1016/j.biortech.2025.132596

Saskia T. Baur , Sarah Schulz , Joshua B. McCluskey , José Antonio Velázquez Gómez , Largus T. Angenent , Bastian Molitor

{"title":"Deletion of aldehyde:ferredoxin oxidoreductase-encoding genes in Clostridium ljungdahlii results in changes in product spectrum with various carbon sources","authors":"Saskia T. Baur , Sarah Schulz , Joshua B. McCluskey , José Antonio Velázquez Gómez , Largus T. Angenent , Bastian Molitor","doi":"10.1016/j.biortech.2025.132596","DOIUrl":"10.1016/j.biortech.2025.132596","url":null,"abstract":"<div><div>Biofuels, such as ethanol, can be produced by the microbial fermentation of waste gases that contain carbon dioxide (CO<sub>2</sub>) and carbon monoxide (CO). The acetogenic model microbe <em>Clostridium ljungdahlii</em> converts those substrates into acetyl-CoA with the Wood-Ljungdahl pathway. During autotrophic conditions, acetyl-CoA can be reduced further to ethanol <em>via</em> acetic acid by the enzymes aldehyde:ferredoxin oxidoreductase (AOR) and alcohol dehydrogenase. Here, the genes encoding both tungsten-dependent AORs (<em>aor1</em>, CLJU_c20110 and <em>aor2</em>, CLJU_c20210) were deleted from the genome of <em>C. ljungdahlii</em>. The effects on the product spectrum of the individual and double deletion strains were investigated. Most pronounced, ethanol formation was enhanced for <em>C. ljungdahlii</em> Δ<em>aor1</em> with different carbon sources, that is, fructose, hydrogen (H<sub>2</sub>) and CO<sub>2</sub>, and CO. The lowest and highest ethanol:acetic acid ratio was detected during growth with H<sub>2</sub>/CO<sub>2</sub> and CO, respectively. Oscillating patterns were observed during growth with CO, underpinning the importance of a balanced redox metabolism.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132596"},"PeriodicalIF":9.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0