Bioresource Technology最新文献_第5页

Valorization of apple tree pruning residues via ozone micro-nano-bubble water pretreatment and Pleurotus ostreatus fermentation. 臭氧微纳泡水预处理和平菇发酵对苹果树修剪残留物的价值评价。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136088

Ji Ju, Jingwen Cao, Xuekai Wang, Xinyi Li, Yanli Lin, Quanmin Dong, Yi Xiong, Fuyu Yang

{"title":"Valorization of apple tree pruning residues via ozone micro-nano-bubble water pretreatment and Pleurotus ostreatus fermentation.","authors":"Ji Ju, Jingwen Cao, Xuekai Wang, Xinyi Li, Yanli Lin, Quanmin Dong, Yi Xiong, Fuyu Yang","doi":"10.1016/j.biortech.2026.136088","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136088","url":null,"abstract":"Lignocellulose recalcitrance and competition from indigenous microorganisms constrain the conversion of apple tree pruning residues (ATPR) into feed by Pleurotus ostreatus. This study compared ozone micro-nano-bubble water (O3-MNBW) and atmospheric intermittent steam sterilization (SS) as pretreatment routes for ATPR fermentation, assessing substrate conversion, lamb growth performance and modelled feed costs. Dry matter recovery reached 85 % under the O3-MNBW route, compared with 79 % under SS. The apparent increases of crude protein mass were 22 % under the O3-MNBW route. Cellulose and hemicellulose degradation reached 49 % and 46 %, respectively, whereas lignin was relatively enriched. Glucose, xylose and total free amino acid concentrations were 1863.34 mg kg-1, 252.87 mg kg-1 and 630.64 ng mg-1 on day 30, respectively. Under SS, lignin and hemicellulose degradation reached 45 % and 63 %, respectively, exceeding cellulose degradation. Metabolomic features assigned to central carbon metabolism and amino acid-related pathways varied with pretreatment route and fermentation time. Fermented ATPR replaced corn straw at 10 % of total dietary dry matter, with no significant differences in lamb growth performance over 45 days. Modelled feed costs per kilogram of body weight gain were 11 % and 9 % lower than in the control for O3-MNBW and SS, respectively. These findings support the use of O3-MNBW pretreatment followed by P. ostreatus fermentation to produce feed for partial replacement of corn straw in lamb diets.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136088"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic small regulatory RNA guided pathway attenuation enables high-level riboflavin production in Escherichia coli. 系统的小调控RNA引导途径衰减使大肠杆菌产生高水平的核黄素。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136087

Junyuan Hou, Zhuohang Yang, Wenya Hu, Shaolan Zou, Zhiwen Wang, Tao Chen

{"title":"Systematic small regulatory RNA guided pathway attenuation enables high-level riboflavin production in Escherichia coli.","authors":"Junyuan Hou, Zhuohang Yang, Wenya Hu, Shaolan Zou, Zhiwen Wang, Tao Chen","doi":"10.1016/j.biortech.2026.136087","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136087","url":null,"abstract":"Riboflavin is an essential vitamin widely used in pharmaceutical and feed applications, and its industrial production relies primarily on microbial fermentation. However, riboflavin biosynthesis in Escherichia coli is constrained by precursor availability and broader physiological regulation. In this study, a small regulatory RNA (sRNA)-mediated attenuation strategy was employed to systematically screen 91 genes across five functional modules. Twenty-three targets increased riboflavin production by more than 20%, and five key targets were further validated through genome-level modification. Combinatorial attenuation of rpe and yrfG, optimization of the nrfA-targeting sRNA expression cassette, and chromosomal reinforcement of the riboflavin operon were subsequently integrated to construct strain SN49M. Following fermentation medium optimization, SN49M produced 3153.3 mg/L riboflavin at 46 h in shake-flask cultivation with a yield of 193.4 mg/g glucose. In fed-batch fermentation, SN49M reached 20.5 g/L riboflavin within 72 h, with an overall yield of 178.9 mg/g glucose, representing the highest reported glucose-based riboflavin yield achieved by microbial fermentation. This work establishes a systematic and tunable framework for discovering and integrating metabolic and physiological perturbations, providing an effective strategy for improving carbon efficiency in microbial riboflavin production.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136087"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Chlamydomonas reinhardtii as a microalgal cell factory for recombinant human serum albumin production. 莱茵衣藻作为重组人血清白蛋白生产的微藻细胞工厂。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136084

Yunting Shi, Sihan Lu, Kaikai Zhang, Zhiyong Liu, Suihao Yan, Haiyang Shi, Longjiang Yu, Weijie Wang, Jiangfeng Zhang, Yuyong Hou, Lei Zhao

{"title":"Engineering Chlamydomonas reinhardtii as a microalgal cell factory for recombinant human serum albumin production.","authors":"Yunting Shi, Sihan Lu, Kaikai Zhang, Zhiyong Liu, Suihao Yan, Haiyang Shi, Longjiang Yu, Weijie Wang, Jiangfeng Zhang, Yuyong Hou, Lei Zhao","doi":"10.1016/j.biortech.2026.136084","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136084","url":null,"abstract":"Human serum albumin (HSA) is an important biopharmaceutical protein, but its recombinant production is still limited by low productivity and high manufacturing cost in conventional expression systems. Here, we engineered Chlamydomonas as a microalgal cell factory for recombinant HSA (rHSA) production. Expression cassette optimization and sequential nuclear transformation were employed to enhance transgene expression, leading to the identification of a high-producing transformant, eCrHSA-3. To further improve production performance, a three-stage pH-controlled fed-batch strategy was developed and successfully implemented in a 5 L bioreactor. Under optimized conditions, biomass reached 48.9 g/L after 10 days of cultivation, with rHSA accumulating to 10.0 mg/L. The identity of the purified recombinant protein was verified by mass spectrometry. Circular dichroism (CD) spectroscopy and 8-anilino-1-naphthalenesulfonic acid (ANS)-binding fluorescence assays revealed similar secondary structure and surface-hydrophobicity features to those of commercially purified human serum albumin, suggesting similarities in their structural characteristics. This study integrates genetic and process engineering for microalgal recombinant protein production and provides a laboratory-scale proof-of-concept for high-density rHSA biosynthesis, highlighting the potential of C. reinhardtii as an alternative host for recombinant protein production.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136084"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Apparent partial denitrification and anammox contribution to nitrogen removal in full-scale multistage anoxic-oxic system treating food waste digestate" [Biores. Tech. 464 (2027) 135951]. “明显的部分反硝化和厌氧氨氧化对全规模多级厌氧-厌氧系统处理食物垃圾消化液中氮去除的贡献”的更正[Biores]。科技。464(2027)135951]。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136061

Wen-Bo Liang, Ming-Rui Shao, Xi-Hui Fan, Dai-Xin Liu, Zhi-Shui Liang, Xiao-Li Yang

引用次数: 0

NH₄Cl-tailored ZIF-67-derived Co/N carbon for enhanced neutral oxygen reduction in microbial fuel cells. nh4cl定制的zif -67衍生Co/N碳用于微生物燃料电池中增强中性氧还原。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136093

Linzhe Huang, Jindi Yang, Kaifan Xiao, Lei Huang, Chengzhi Wang, Hongguo Zhang, How Yong Ng

{"title":"NH4Cl-tailored ZIF-67-derived Co/N carbon for enhanced neutral oxygen reduction in microbial fuel cells.","authors":"Linzhe Huang, Jindi Yang, Kaifan Xiao, Lei Huang, Chengzhi Wang, Hongguo Zhang, How Yong Ng","doi":"10.1016/j.biortech.2026.136093","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136093","url":null,"abstract":"Developing non-precious cathode catalysts for neutral oxygen reduction is critical to improving microbial fuel cell performance. Here, NH4Cl-assisted secondary pyrolysis was used to reconstruct ZIF-67-derived Co/N-doped carbon and regulate Co/N surface environments. The optimized CoNC-1 combined an open porous framework, abundant defects, improved wettability, and favorable pyridinic N/Co-Nx-containing surface configurations. Density functional theory indicated adsorbate-induced Co 3d redistribution and a theoretical overpotential of 0.244 V for the four-electron oxygen reduction pathway at the Co-N-C model site. In PBS condition, CoNC-1 delivered a half-wave potential of 0.592 V vs. RHE, a limiting current density of 6.37 mA·cm-2, an H2O2 yield of 5.18%, and an electron-transfer number of approximately 3.90. As an air-cathode catalyst, CoNC-1 achieved a maximum power density of 681.5 ± 5.99 mW m-2, 87.2% COD removal, and 15.3% coulombic efficiency. These results demonstrate that coupling framework reconstruction with surface Co/N regulation improves neutral ORR and wastewater-to-electricity conversion.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136093"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Region-specific engineering of a bifunctional alcohol dehydrogenase enables efficient single-enzyme sequential oxidation. 双功能醇脱氢酶的区域特异性工程实现了高效的单酶顺序氧化。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136082

Kun Cao, Zhao Wu, Xiao Ma, Yishu Li, Jiangfeng Ma, Bin Wu, Jialing Wang

{"title":"Region-specific engineering of a bifunctional alcohol dehydrogenase enables efficient single-enzyme sequential oxidation.","authors":"Kun Cao, Zhao Wu, Xiao Ma, Yishu Li, Jiangfeng Ma, Bin Wu, Jialing Wang","doi":"10.1016/j.biortech.2026.136082","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136082","url":null,"abstract":"Efficient biocatalytic oxidation of primary alcohols to carboxylic acids is attractive for sustainable synthesis, but complete alcohol-to-acid conversion usually requires coordinated control of sequential oxidation steps and continuous regeneration of oxidized cofactors. Here, we report a region-specific engineering strategy to improve GtADH2, a bifunctional NAD+-dependent alcohol dehydrogenase capable of catalyzing single-enzyme sequential oxidation of terminal hydroxy fatty acids. Guided by a GtADH2-12-hydroxydodecanoic acid-NAD+ ternary complex model, the substrate-binding pocket and NAD+-binding region were separately engineered and then combinatorially integrated. The resulting variant M4-1 (N147T/N8Q/S274D/D268Q) exhibited a 10.2-fold increase in activity and an approximately 17.3-fold improvement in catalytic efficiency toward 12-hydroxydodecanoic acid compared with the wild type. Kinetic analysis showed that M4-1 improved both substrate utilization and NAD+-dependent catalytic efficiency, while molecular dynamics simulations supported enhanced NAD+ stabilization and more favorable hydride-transfer geometry. To sustain sequential oxidation under low cofactor loading, M4-1 was integrated with a flavinium-assisted NAD+ recycling module. Using only 0.2 mM NAD+, the M4-1/FocH system converted 2 mM 12-hydroxydodecanoic acid to 1.96 mM dodecanedioic acid within 2 h. M4-1 also showed improved activity toward terminal hydroxy fatty acids with different chain lengths. This work demonstrates that region-specific engineering of substrate- and cofactor-associated regions, combined with flavinium-assisted cofactor recycling, provides an effective single-enzyme platform for NAD+-dependent alcohol-to-acid oxidation.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136082"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modular reconstruction and multi-level regulation of Escherichia coli for efficient uridine production. 高效尿苷生产的大肠杆菌模块化重组和多级调控。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136086

Qisheng Sun, Yujie Huo, Yongming Chen, Mi Tang, Yixuan Zhao, Landuo Sui, Peng Wen, Taowei Yang, Xuewei Pan, Zhiming Rao

{"title":"Modular reconstruction and multi-level regulation of Escherichia coli for efficient uridine production.","authors":"Qisheng Sun, Yujie Huo, Yongming Chen, Mi Tang, Yixuan Zhao, Landuo Sui, Peng Wen, Taowei Yang, Xuewei Pan, Zhiming Rao","doi":"10.1016/j.biortech.2026.136086","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136086","url":null,"abstract":"Uridine is an important pyrimidine nucleoside with extensive applications in pharmaceuticals, food, and biomanufacturing. However, the microbial synthesis of uridine is still hindered by unsatisfactory titer, yield, and productivity. Here, modular reconstruction combined with multilevel metabolic engineering was employed to design and construct a high-yielding Escherichia coli platform strain for efficient uridine synthesis. Enhancing the pyrimidine biosynthetic capacity was initially achieved by introducing a pyrimidine operon derived from Bacillus subtilis and disrupting the uridine degradation pathway, thereby promoting de novo uridine formation. Further metabolic optimization focused on improving precursor availability and product accumulation by strengthening carbamoyl phosphate supply, increasing pyrimidine operon dosage, reducing uridine uptake, and optimizing precursor supply. To balance cellular growth and uridine synthesis, the Esa quorum sensing system was utilized for dynamic regulation of thrA expression, while overexpression of the 5'-nucleotidase YjjG substantially increased uridine production. Through the combination of these strategies, the engineered strain U20-2 achieved a uridine titer of 17.99 g/L in shake flask fermentation. An optimized two-stage temperature-controlled fed-batch fermentation process further increased uridine production to 105.68 g/L within 82 h, with a yield and productivity of 0.265 g/g and 1.28 g/L/h, respectively. This represents the highest titer reported to date for glucose-based uridine production in E. coli, and notably, the entire fermentation process was carried out without exogenous inducers or antibiotics. Overall, this study offers a highly promising and practical strategy for the efficient biomanufacturing of uridine and related pyrimidine nucleosides.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136086"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-chain perfluorobutyric acid (PFBA) disrupts anaerobic ammonium oxidation in riverbed sediments and reshapes the functional microbial community. 短链全氟丁酸（PFBA）破坏河床沉积物中的厌氧铵氧化，重塑功能性微生物群落。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136077

Shengnan Zhang, Qiong Wang, Xiaojing Zhang, Zhaoyang Tian, Xinyue Xu, Siyu Wang, Guangchen Lv, Yeyang Chen, Yongpeng Ma, Aihua Gao

{"title":"Short-chain perfluorobutyric acid (PFBA) disrupts anaerobic ammonium oxidation in riverbed sediments and reshapes the functional microbial community.","authors":"Shengnan Zhang, Qiong Wang, Xiaojing Zhang, Zhaoyang Tian, Xinyue Xu, Siyu Wang, Guangchen Lv, Yeyang Chen, Yongpeng Ma, Aihua Gao","doi":"10.1016/j.biortech.2026.136077","DOIUrl":"10.1016/j.biortech.2026.136077","url":null,"abstract":"Perfluorobutanoic acid (PFBA), a representative short-chain perfluoroalkyl carboxylic acid, is increasingly detected in aquatic environments, yet its impacts on anaerobic nitrogen cycling remain poorly understood. Here, a laboratory-scale simulated river sediment system was established to investigate the concentration-dependent effects of PFBA (1 ng/L-50 mg/L) on Anammox performance, microbial communities, functional genes, and PFBA transformation. Low PFBA concentrations (≤1 μg/L) stimulated nitrogen removal, with NH4+-N conversion and NO2--N removal increasing by 30.8% and 38.9%, respectively. In contrast, inhibition emerged at 1 mg/L and became pronounced at ≥ 10 mg/L. At 50 mg/L, NO2--N and TN removal rates decreased to 7.58 and 5.22 μg/(g·h), respectively, and remained suppressed during recovery. High PFBA exposure reduced extracellular polymeric substances (EPS) -associated protein content and key Anammox enzyme activities, accompanied by downregulation of hao, hdh, and hzs and increased relative abundance of nrf, indicating a shift from autotrophic Anammox toward heterotrophic dissimilatory nitrate reduction to ammonium (DNRA). Notably, 91.7% of PFBA was apparently transformed at 50 mg/L, with shorter-chain products such as perfluoropropanoic acid and trifluoroacetic acid being qualitatively identified, suggesting sequential dealkylation and decarboxylation. However, a complete mass balance was not established because the transformation products were not quantified. These findings demonstrate that PFBA exerts concentration-dependent effects on sedimentary nitrogen cycling by disrupting Anammox metabolism and reshaping microbial functional pathways, providing new insights into per- and polyfluoroalkyl substances (PFAS) ecological risks and sediment bioremediation.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136077"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar enhance biobutanol production by modulating redox cofactors and allocating reducing power. 生物炭通过调节氧化还原辅助因子和分配还原力来增强生物丁醇的生产。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-17 DOI: 10.1016/j.biortech.2026.136091

Yu Chen, Xingyang Cheng, Yue Lu, Lin Tang, Jie Yuan

{"title":"Biochar enhance biobutanol production by modulating redox cofactors and allocating reducing power.","authors":"Yu Chen, Xingyang Cheng, Yue Lu, Lin Tang, Jie Yuan","doi":"10.1016/j.biortech.2026.136091","DOIUrl":"10.1016/j.biortech.2026.136091","url":null,"abstract":"Acetone-butanol-ethanol (ABE) fermentation is a promising route for converting carbohydrate-rich feedstocks into biobutanol, but its efficiency is fundamentally constrained by the inefficient intracellular allocation of reducing power. Biochar addition can improve fermentation performance, but the mechanisms underlying this enhancement remain insufficiently resolved. Here, we investigated the fermentation performance, intracellular redox cofactors, and proteomic responses of Clostridium acetobutylicum to corn straw biochar prepared at different pyrolysis temperatures. We found that the pyrolysis temperature of the biochar (optimally 600℃) significantly dictated the enhancement of biobutanol production. Separation of water-soluble organic carbon from bulk biochar showed that the soluble fraction alone did not explain the enhancement. Comparable butanol production in direct-contact and Transwell systems further showed that biochar-cell contact was unnecessary. Additional experiments with acid-pretreated and oxygen-oxidized biochar suggest that extracellular environmental regulation may contribute to the enhanced butanol production. Intracellularly, biochar upregulated enzymes involved in NAD biosynthesis, glycolysis, acid reassimilation, and solventogenesis. This metabolic shift successfully redirected carbon flux and reducing equivalents away from competing pathways, such as hydrogen evolution, and channeled them toward solvent biosynthesis, ultimately increasing biobutanol production by 30%. Based on these mechanistic insights, we propose a compartmentalized biochar unit design for scalable implementation. These findings bridge the fundamental knowledge gap regarding biochar-mediated redox regulation and offer a robust, scalable engineering strategy for optimizing anaerobic bioproduction.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136091"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of whey anaerobic digestion stability based on organic loading rate fluctuation: In terms of syntrophic volatile fatty acid degradation and microbial signals of recovery. 基于有机负荷率波动的乳清厌氧消化稳定性评价：基于合成性挥发性脂肪酸降解和微生物信号的恢复。

IF 8.2 1区环境科学与生态学

Bioresource Technology Pub Date : 2026-10-16 DOI: 10.1016/j.biortech.2026.136073

Sung Il Yu, Juhee Shin, Tergel Dalantai, Yong-Uk Shin, Chaeyoung Rhee, Kyung Hwa Cho, Seung Gu Shin, Hyokwan Bae

{"title":"Assessment of whey anaerobic digestion stability based on organic loading rate fluctuation: In terms of syntrophic volatile fatty acid degradation and microbial signals of recovery.","authors":"Sung Il Yu, Juhee Shin, Tergel Dalantai, Yong-Uk Shin, Chaeyoung Rhee, Kyung Hwa Cho, Seung Gu Shin, Hyokwan Bae","doi":"10.1016/j.biortech.2026.136073","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136073","url":null,"abstract":"Anaerobic digestion (AD) is a promising method for treating organic waste, but it requires thorough management. While it is known that organic loading fluctuations affect anaerobic digestion, their specific impact has not been verified. Therefore, this study hypothesized that the response to overload differs depending on the organic loading rate (OLR) strategy to which anaerobic digesters are adapted. To test this hypothesis, sequencing batch reactors were operated using whey as the substrate. Five operational conditions were compared under three fixed OLRs of 0.25, 1.0, and 2.0 g COD/L∙day (i.e., low-L, medium-M, and high-H, respectively) and two dynamic OLR regimes of stepwise oscillation (SO; M-L-M-H conditions) and drastic oscillation (DO; L-H conditions). After the initial operation period, all reactors were subjected to the same overload, recovery, and treatment (C-control, B-bioaugmentation). The LB and SOB reactors showed marked pH decreases and caproic acid accumulation, whereas the HB and DOB reactors showed delayed propanoic acid degradation. In contrast, the MB reactor exhibited superior performance in terms of volatile fatty acid (VFA) removal and pH recovery on D159. The microbial community changed dynamically according to the OLR history. PICRUSt2 analysis suggested that syntrophic VFA degradation and amino acid metabolism were affected by the OLR histories. Network analysis revealed putative associations between minor syntrophic acetate-oxidizing bacteria and Methanosarcina during recovery. Additionally, the connection of protein-related consortia (Aminobacterium-Methanomassiliicoccus) was inferred by network analysis. Overall, the results of this study indicate that prior OLR history shapes overload resilience by altering VFA degradation trajectories and microbial recovery patterns.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136073"},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0