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Enhancement of the yield of poly (ethylene terephthalate) hydrolase production using cell membrane protection strategy. 利用细胞膜保护策略提高聚对苯二甲酸乙酯水解酶的产率。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-11-28 DOI: 10.1016/j.biortech.2024.131903
Xiao-Qian Chen, De-Ming Rao, Xing-Yu Zhou, Yang Li, Xiao-Min Zhao, De-Min Kong, Han Xu, Chu-Qi Feng, Lei Wang, Ling-Qia Su, Zheng-Fei Yan, Jing Wu
{"title":"Enhancement of the yield of poly (ethylene terephthalate) hydrolase production using cell membrane protection strategy.","authors":"Xiao-Qian Chen, De-Ming Rao, Xing-Yu Zhou, Yang Li, Xiao-Min Zhao, De-Min Kong, Han Xu, Chu-Qi Feng, Lei Wang, Ling-Qia Su, Zheng-Fei Yan, Jing Wu","doi":"10.1016/j.biortech.2024.131903","DOIUrl":"10.1016/j.biortech.2024.131903","url":null,"abstract":"<p><p>Biodegradation, particularly via enzymatic degradation, has emerged as an efficient and eco-friendly solution for Poly (ethylene terephthalate) (PET) pollution. The production of PET hydrolases plays a role in the large-scale enzymatic degradation. However, an effective variant, 4Mz, derived from Thermobifida fusca cutinase (Tfu_0883), was previously associated with a significant reduction in yield when compared to the wild-type enzyme. In this study, a novel cell membrane protection strategy was developed to enhance the yield of 4Mz. This approach increased the yield of 4Mz by 18.2-fold from shaken flasks to 3-L bioreactors, reaching a yield of 3.1 g·L<sup>-1</sup>, the highest yield of a PET hydrolase described thus far. In addition, the raw culture broth from 4Mz was applied directly for the enzymatic degradation of PET bottles, achieving a 91.2 % degradation rate. These advancements render the large-scale enzymatic degradation of PET more feasible, thus contributing to the more sustainable management of plastic waste.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131903"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fueling the protein transition: Can waste-derived ethanol enable efficient and high-quality microbial protein production? 促进蛋白质转化:废物衍生乙醇能否实现高效、高质量的微生物蛋白质生产?
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.1016/j.biortech.2024.131990
L Van Peteghem, S Matassa, M Sakarika
{"title":"Fueling the protein transition: Can waste-derived ethanol enable efficient and high-quality microbial protein production?","authors":"L Van Peteghem, S Matassa, M Sakarika","doi":"10.1016/j.biortech.2024.131990","DOIUrl":"10.1016/j.biortech.2024.131990","url":null,"abstract":"<p><p>Meeting the protein needs of a growing population will require significant resources. In this context, microbial protein (MP) offers a nutritious and versatile protein source from recovered resources. This meta-analysis of over 100 studies examines the efficiency and nutritional quality of MP production using ethanol. Ethanol, a feedstock derived from CO<sub>2</sub> and biological waste, is used by various microorganisms, and has an established role in the food sector. Results show that ethanol-based MP production is technically feasible for food applications, reaching biomass concentrations of 14-230 g/L and productivities of 11-13 g/L/h. The protein content of MP correlates with productivity, and the nutritional quality of ethanol-grown MP matches common sources like pork and tofu. Lastly, operational choices affect the techno-economic feasibility of using waste-derived ethanol and other recovered resources. This meta-analysis highlights the potential of ethanol-grown MP, though further research is needed to close existing knowledge gaps.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131990"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Achieving synergistic enhancement in the anaerobic digestion of corn straw by (CH4 + CO2) nanobubbles in conjunction with optimized particle sizes. (CH4 + CO2)纳米气泡与优化粒径协同促进玉米秸秆厌氧消化
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-17 DOI: 10.1016/j.biortech.2024.131997
Zhiqiang Cui, Pengfei Li, Xiaohui Pan, Yongkang Yuan, Gang Li, Youzhou Jiao, Francesco Petracchini, Tingting Hou, Chao He
{"title":"Achieving synergistic enhancement in the anaerobic digestion of corn straw by (CH<sub>4</sub> + CO<sub>2</sub>) nanobubbles in conjunction with optimized particle sizes.","authors":"Zhiqiang Cui, Pengfei Li, Xiaohui Pan, Yongkang Yuan, Gang Li, Youzhou Jiao, Francesco Petracchini, Tingting Hou, Chao He","doi":"10.1016/j.biortech.2024.131997","DOIUrl":"10.1016/j.biortech.2024.131997","url":null,"abstract":"<p><p>Nanobubbles (NBs) technology has been proven to promote methane production from anaerobic digestion (AD). In this study, the synergistic effects of (CH<sub>4</sub> + CO<sub>2</sub>)-nanobubble water ((CH<sub>4</sub> + CO<sub>2</sub>)-NBW) combined with varying particle sizes of corn straw on the AD were investigated. As findings, adding (CH<sub>4</sub> + CO<sub>2</sub>)-NBW effectively promoted the methane production from AD of corn straw with different particle sizes. The maximum cumulative methane yield (186.42 mL/ g-volatile solids) was achieved in Group a with the addition of (CH<sub>4</sub> + CO<sub>2</sub>)-NBW, representing a 16.89 % increase compared to the control. Furthermore, (CH<sub>4</sub> + CO<sub>2</sub>)-NBW could enhance the enzymatic activity. The activities of β-glucosidase and coenzyme F<sub>420</sub> were increased by 6.70 % and 11.48 %, respectively. The results of microbial community structure revealed that the addition of (CH<sub>4</sub> + CO<sub>2</sub>)-NBW could improve the abundance of dominant bacteria (norank_JS1, norank_Aminicenantales, and Bacteroidetes_vadinHA17) and archaea (Methanomassiliicoccaceae, Methanobacteriaceae, and norank_Bathyarchaeia). This study provides new insights into the application of nanobubbles in the AD of biomass.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131997"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Process performance of in-situ bio-methanation for co-digestion of sewage sludge and lactic acid, aiming to utilize waste poly-lactic acid as methane. 原位生物甲烷化污泥与乳酸共消化的工艺性能,目的是利用废聚乳酸作为甲烷。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI: 10.1016/j.biortech.2024.131945
Shinya Akimoto, Jun Tsubota, Shinya Tagawa, Tatsuaki Hirase, Irini Angelidaki, Taira Hidaka, Taku Fujiwara
{"title":"Process performance of in-situ bio-methanation for co-digestion of sewage sludge and lactic acid, aiming to utilize waste poly-lactic acid as methane.","authors":"Shinya Akimoto, Jun Tsubota, Shinya Tagawa, Tatsuaki Hirase, Irini Angelidaki, Taira Hidaka, Taku Fujiwara","doi":"10.1016/j.biortech.2024.131945","DOIUrl":"10.1016/j.biortech.2024.131945","url":null,"abstract":"<p><p>This study examined hydrogen conversion efficiency and operational stability in pilot-scale in-situ bio-methanation during the co-digestion of sewage sludge and lactic acid (partially derived from waste poly-lactic acid). Parallel laboratory-scale experiments were also conducted. In the pilot, hydrogen conversion efficiency decreased from 98.9 % to 84.4 % as the hydrogen feed rate increased from 240 to 1,200 mL/L<sub>R</sub>/d. Conversely, laboratory experiments maintained efficiencies above 95 % at a feed rate of 3,600 mL/L<sub>R</sub>/d, suggesting that hydrogen gas-liquid transfer limited hydrogen conversion efficiency in the pilot. Lactic acid degradation was observed both with and without hydrogen injection in the pilot. Methane yields from the acid were 310 ± 30 and 300 ± 30 mL/g (chemical oxygen demand (COD))-added, close to the theoretical methane yield (350 mL/gCOD). These results demonstrate the importance of hydrogen gas-liquid transfer when scaling up bio-methanation processes. Moreover, they showed the potential of waste poly-lactic acid as a methane source.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131945"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond waste in agriculture: Feedstock characterization for thermochemical conversion based on potato above-ground biomass. 超越农业中的废物:基于马铃薯地上生物量的热化学转化的原料特性。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI: 10.1016/j.biortech.2024.131943
Jhoan Chávez, Jianbing Li
{"title":"Beyond waste in agriculture: Feedstock characterization for thermochemical conversion based on potato above-ground biomass.","authors":"Jhoan Chávez, Jianbing Li","doi":"10.1016/j.biortech.2024.131943","DOIUrl":"10.1016/j.biortech.2024.131943","url":null,"abstract":"<p><p>Agricultural residues represent a valuable opportunity to develop circular bioeconomic systems centered on biomass. Characterizing this type of biomass can alleviate the pressure on current biomass sources (e.g., in forests and their biodiversity), enhance agricultural waste management, and reduce crop field emissions. Thus, this study aimed to evaluate the potential of agricultural plant-based residues as feedstock for thermochemical conversion processes, focusing on potato above-ground biomass to enhance herbaceous characterization. The gravimetric characterization of this type of biomass revealed a water content of 89 % for potato above-ground biomass with differences per plant section. Biomass abundance was also measured, showing that well-developed leaves and main stems were more plentiful. After 70 days after planting (DAP), maximum plant development was achieved, differing from heights development at 40 DAP. Hence, identifying specific times for residue recovery can help develop strategies for biomass recovery, thereby reducing pretreatment costs following the selection of suitable conversion technologies.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131943"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and application of a comprehensive numerical simulation model for microalgal pneumatic photobioreactors. 微藻气动光生物反应器综合数值模拟模型的开发与应用。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-06 DOI: 10.1016/j.biortech.2024.131962
Ruofan Wang, Xiaobo Yao, Raphael Semiat, Bo Kong
{"title":"Development and application of a comprehensive numerical simulation model for microalgal pneumatic photobioreactors.","authors":"Ruofan Wang, Xiaobo Yao, Raphael Semiat, Bo Kong","doi":"10.1016/j.biortech.2024.131962","DOIUrl":"10.1016/j.biortech.2024.131962","url":null,"abstract":"<p><p>A novel and comprehensive multiphysics numerical simulation model for microalgal pneumatic photobioreactors was developed using the open-source Computational Fluid Dynamics (CFD) software OpenFOAM. This model integrates three distinct submodels: multiphase fluid dynamics, light transport, and microalgae growth. In particular, the light transport submodel accounts for light scattering caused by air bubbles. The model effectively addressed the challenges associated with the disparate time scales of the submodels, and its accuracy was validated by comparing with several experiments in different configurations. This study focuses on how an outdoor flat plate photobioreactor can maximize solar energy utilization. The results indicate that the daily productivity of the light-intensity adaptive reactor increased by 12.31 % to 26.31 % compared to those at constant biomass concentrations, demonstrating significant potential for employing this model in conjunction with automated systems to enhance productivity.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131962"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing an adapted strain of Clostridium carboxidivorans to unlock hexanol production from carbon dioxide and hydrogen in elevated-pressure stirred tank reactors. 利用一种适应的碳梭菌菌株,在高压搅拌罐式反应器中从二氧化碳和氢气中释放己醇。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-09 DOI: 10.1016/j.biortech.2024.131966
G Antonicelli, N Vasile, E Piro, S Fraterrigo Garofalo, B Menin, F Verga, F Pirri, V Agostino
{"title":"Harnessing an adapted strain of Clostridium carboxidivorans to unlock hexanol production from carbon dioxide and hydrogen in elevated-pressure stirred tank reactors.","authors":"G Antonicelli, N Vasile, E Piro, S Fraterrigo Garofalo, B Menin, F Verga, F Pirri, V Agostino","doi":"10.1016/j.biortech.2024.131966","DOIUrl":"10.1016/j.biortech.2024.131966","url":null,"abstract":"<p><p>To successfully scale-up the production of bio-based building blocks through CO<sub>2</sub> and H<sub>2</sub>-based gas fermentation, it is crucial to deeply understand and control the microbial catalyst response to the bioreactor environment. This study investigates the effects of key process parameters, such as CO<sub>2</sub> and H<sub>2</sub> partial pressures, gas feeding strategies, and mixture composition, on the production pathways of an evolved Clostridium carboxidivorans strain. The ultimate goal is to optimize 1-hexanol production in elevated-pressure stirred-tank reactors. Continuous gas feeding enhanced acetogenic and solventogenic metabolisms, while gas-limited conditions promoted chain elongation to caproic acid. An optimized process, combining an initial gas-limited step followed by a continuous gas phase, increased 1-hexanol production, achieving a maximum biomass-specific productivity of 0.9 g g<sub>CDW</sub><sup>-1</sup> day<sup>-1</sup>. In-situ product extraction improved 1-hexanol carbon selectivity to an unprecedented 60 %. These findings demonstrate the potential of CO<sub>2</sub> and H<sub>2</sub>-fed fermentation to produce high-value chemicals other than ethanol and acetate.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131966"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteinase K impact on anaerobic co-digestion of modified biodegradable plastic and food waste: Step-by-step analysis with microorganism. 蛋白酶 K 对改性生物可降解塑料和厨余垃圾厌氧协同消化的影响:利用微生物逐步分析。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI: 10.1016/j.biortech.2024.131984
Wenjie Liu, Shizhuo Wang, Songting He, Yang Shi, Cheng Hou, Yuanbo Song, Tao Zhang, Yalei Zhang, Zheng Shen
{"title":"Proteinase K impact on anaerobic co-digestion of modified biodegradable plastic and food waste: Step-by-step analysis with microorganism.","authors":"Wenjie Liu, Shizhuo Wang, Songting He, Yang Shi, Cheng Hou, Yuanbo Song, Tao Zhang, Yalei Zhang, Zheng Shen","doi":"10.1016/j.biortech.2024.131984","DOIUrl":"10.1016/j.biortech.2024.131984","url":null,"abstract":"<p><p>This study was designed to explore the key impact of Proteinase K (PK) on every step of anaerobic co-digestion. The results of step-by-step experiments indicated that PK promoted the hydrolysis of biodegradable plastic by initiating self-hydrolysis reactions, directly promoting the hydrolysis step of anaerobic co-digestion. Subsequently, PK indirectly promoted the acidogenesis and acetogenesis steps by impacting the proliferation of acid-producing bacteria. Besides, it could also hydrolyze PLA. Thus, the lactic acid content peaked at 255.7 mg/L on the 5th day, representing an increase of 35.9 % compared to the condition without PK. Finally, PK indirectly promoted the methanogenesis step through its impact on the composition of methanogenic bacteria. This led to more food waste being digested into methane, 41.5 % compared to the condition without PK. This work served as an essential foundation for advancing the application of PK modified BP as a replacement for traditional plastics.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131984"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combining quorum quenching by Rhodococcus sp. BH4 and Acinetobacter sp. DKY-1 to control biofouling in membrane bioreactors. 结合红球菌BH4和不动杆菌DKY-1的群体猝灭控制膜生物反应器中的生物污染。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-15 DOI: 10.1016/j.biortech.2024.131981
Yanyao Huang, Xueman Zheng, Yunshi Feng, Xingtong Feng, Fangfang Xu
{"title":"Combining quorum quenching by Rhodococcus sp. BH4 and Acinetobacter sp. DKY-1 to control biofouling in membrane bioreactors.","authors":"Yanyao Huang, Xueman Zheng, Yunshi Feng, Xingtong Feng, Fangfang Xu","doi":"10.1016/j.biortech.2024.131981","DOIUrl":"10.1016/j.biortech.2024.131981","url":null,"abstract":"<p><p>This study investigates a novel approach to mitigate biofouling in membrane bioreactors (MBRs) using a combinational quorum quenching (QQ) strategy. Rhodococcus sp. BH4 and Acinetobacter sp. DKY-1 were employed to disrupt intraspecies N-acyl-homoserine lactones (AHL) and interspecies autoinducer-2 (AI-2) quorum sensing, respectively. BH4 and DKY-1 were immobilized independently and the antibiofouling effects of single QQ beads and 1: 1 mixed QQ beads, both with the same final doses, were compared. While both bead types exhibited high QQ activity, the mixed QQ beads more effectively inhibited microbial biofilm formation, delaying biofouling by two times compared to 1.5 and 1.7 times for the single species beads. Additionally, the mixed QQ MBR demonstrated significantly lower extracellular polymeric substances and a notable reduction in the genus Nitrospira. This combined QQ strategy presents a promising method for enhancing antibiofouling performance in MBRs through targeted disruption of microbial communication.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131981"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing the rhizosphere sponge to smooth pH fluctuations and stabilize contaminant retention in biofiltration system. 利用根瘤海绵平缓 pH 值波动,稳定生物过滤系统中的污染物滞留。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.biortech.2024.131971
Guoliang Wang, Tianying Chi, Ruixiang Li, Tian Li, Xiaolin Zhang
{"title":"Harnessing the rhizosphere sponge to smooth pH fluctuations and stabilize contaminant retention in biofiltration system.","authors":"Guoliang Wang, Tianying Chi, Ruixiang Li, Tian Li, Xiaolin Zhang","doi":"10.1016/j.biortech.2024.131971","DOIUrl":"10.1016/j.biortech.2024.131971","url":null,"abstract":"<p><p>Fluctuating pH conditions can affect heavy metal mobility, thereby limiting the efficiency of biofiltration systems (BS). To address this, we developed an innovative rhizosphere sponge, biochar-based bioreactor (RBB), designed to stabilize Cd<sup>2+</sup> removal across a pH range of 5 to 9. RBB consistently outperformed the control, achieving a notable 91.3 % Cd<sup>2+</sup> removal at pH 5. By creating optimized oxygen and redox zoning, the rhizosphere sponge enhanced both biochar surface reactions and microbial activity. Under acidic conditions, biochar facilitated Fe<sup>2+</sup>/Mn<sup>2+</sup> precipitation into stable (oxy)hydroxides, a process further driven by microbial oxidation. Consequently, RBB accumulated 1.54 times more Fe-Mn oxide-bound Cd than the control, effectively reducing Cd<sup>2+</sup> mobility. Additionally, loosely bound extracellular polymeric substances claimed preferential Cd<sup>2+</sup> sequestration after acidification. The stabilized microecology and increased ecological niches, allowing RBB to better buffer against pH fluctuations, presenting it as a robust solution for sustainable heavy metal remediation in variable environments.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131971"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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