Bioresource Technology最新文献_第4页

Migration behavior of phosphorus during microwave-assisted co-hydrothermal carbonization and co-pyrolysis of chemical sludge and corncob 微波辅助化学污泥与玉米芯共热炭化共热解过程中磷的迁移行为

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132987

Baofeng Wang , Yunxiao Chen , Yaxin Guo , Mukun Xue , Huirong Zhang , Jinglei Cui , Jiawei Wang , Yanxia Guo

{"title":"Migration behavior of phosphorus during microwave-assisted co-hydrothermal carbonization and co-pyrolysis of chemical sludge and corncob","authors":"Baofeng Wang , Yunxiao Chen , Yaxin Guo , Mukun Xue , Huirong Zhang , Jinglei Cui , Jiawei Wang , Yanxia Guo","doi":"10.1016/j.biortech.2025.132987","DOIUrl":"10.1016/j.biortech.2025.132987","url":null,"abstract":"<div><div>Recycling of phosphorus (P) from chemical sludge (CS) is important for alleviating P resources shortage and solving P pollution. In this study, the transformation behavior and the mechanism of P during microwave-assisted co-hydrothermal carbonization (co-HTC) and co-pyrolysis of CS and corncob was investigated. The results showed that microwave could enhance the process of co-HTC and co-pyrolysis, and made P enriched in hydrochar and biochar. Microwaves made the content of orthophosphate (Ortho-P) and pyrophosphate (Pyro-P) in hydrochar and biochar increase, as well as making the organic phosphorus (OP) convert into inorganic phosphorus (IP). Meanwhile, the results also showed that P-containing minerals existed as Al-P and Fe-P in hydrochar and existed as Ca-P and Mg-P minerals in biochar. Furthermore, microwave assistance also could significantly improve the bioavailability of P. The results of this study may provide a basis for the P recovery from chemical sludge, as well as for clean and efficient utilization of hydrochar and biochar.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132987"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated hydrothermal and ball-milling approach for the co-production of glucose, xylose and residual lignin from Moso bamboo 水热和球磨联合生产毛竹中葡萄糖、木糖和残余木质素的研究。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132985

Qianying Yang , Chen Zhang , Lili Shang , Jialong Wen , Shumin Yang

{"title":"An integrated hydrothermal and ball-milling approach for the co-production of glucose, xylose and residual lignin from Moso bamboo","authors":"Qianying Yang , Chen Zhang , Lili Shang , Jialong Wen , Shumin Yang","doi":"10.1016/j.biortech.2025.132985","DOIUrl":"10.1016/j.biortech.2025.132985","url":null,"abstract":"<div><div>Clean and efficient pretreatment is critical for the sustainable development of bamboo industry. In this study, a combination of hydrothermal pretreatment (including AlCl<sub>3</sub> and glycerol) and ball-milling were proposed to efficiently facilitating co-production of xylose, glucose and residual lignin from moso bamboo. The results showed that the ball-milling process before hydrothermal pretreatment destroyed the compact structure of bamboo, and 44.5% xylose was obtained after hydrothermal pretreatment at 150 °C for 30 min. The 5 mins’ ball-milling process significantly reduced the “biomass recalcitrance” of the hydrothermal substrate. After 24 h of rapid enzymatic hydrolysis, the glucose yield increased from 32.6% to 81.3%, compared with 5.8% of the untreated bamboo. Finally, this method realized the effective isolation of enzymatic lignin with high-yield (87.0%), acceptable purity (84.3%) and non-condensed structure. In conclusion, the proposed strategy is a green, clean, and efficient strategy for the high-efficiency deconstruction and high-value utilization of bamboo resources.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132985"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting 微生物介导的Fenton系统对秸秆堆肥过程中木质素转化的协同强化机制

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132981

Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei

{"title":"Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting","authors":"Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei","doi":"10.1016/j.biortech.2025.132981","DOIUrl":"10.1016/j.biortech.2025.132981","url":null,"abstract":"<div><div>This study investigated the synergistic strengthening mechanism of a microbial-mediated Fenton system in lignin depolymerization during composting. The Fenton system was constructed using hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced by lignin-degrading microorganisms along with Fe(Ⅱ). The simultaneous inoculation of bacteria and fungi resulted in optimal enzyme activities related to lignocellulose degradation and lignin degradation rates. Moreover, the addition of 0.5% FeSO<sub>4</sub> further optimized hydroxyl radical (<strong>·</strong>OH) production. Therefore, four treatments were set: CK (control), BHF (bacterial fungi synchronized inoculation), Fe (FeSO<sub>4</sub>), and BHF-Fe (bacterial fungi synchronized inoculation + FeSO<sub>4</sub>). BHF-Fe synergistically drove the Fe(Ⅱ)/Fe(Ⅲ) redox cycle and maintained reactive oxygen species ROS, (superoxide anion, H<sub>2</sub>O<sub>2</sub> and <strong>·</strong>OH) generation. BHF-Fe showed a 22.58% greater lignin loss rate compared to the CK. The correlations between ROS and lignin loss rate were enhanced. Additionally, lignin peroxidase, as an extracellular enzyme, relies on H<sub>2</sub>O<sub>2</sub> for its action on lignin. The activities of laccase and lignin peroxidase were significantly correlated. Lignin decomposition was accelerated through the upregulation of key degrading enzyme genes (<em>Lac</em>, <em>LiP</em>, and <em>MnP</em>). The Fenton system showed attenuated pH dependence relative to conventional systems despite pH and Fe (II) effects on ROS. The BHF-Fe achieves efficient lignin degradation through a synergistic mechanism that integrates Fenton reactions, ROS, biocatalytic enzymatic oxidation and microenvironment compensation. This research offers theoretical and technical backing for developing agricultural waste resource recycling and composting technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132981"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient production of l-rhamnose from catalytic hydrolysis of marine polysaccharide ulvan over carbon embedded sulfonated resins 碳包埋磺化树脂催化水解海洋多糖ulvan高效生产l-鼠李糖

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132977

Milan Arvindbhai Dumraliya , Satish Kumar Nayak , R. Suhail Haq , Sanket Nareshbhai Balar , Nikhilesh Trivedi , Mariappan Mani , S. Dinesh Kumar , V. Veeragurunathan , Lakhya Jyoti Konwar

{"title":"Highly efficient production of l-rhamnose from catalytic hydrolysis of marine polysaccharide ulvan over carbon embedded sulfonated resins","authors":"Milan Arvindbhai Dumraliya , Satish Kumar Nayak , R. Suhail Haq , Sanket Nareshbhai Balar , Nikhilesh Trivedi , Mariappan Mani , S. Dinesh Kumar , V. Veeragurunathan , Lakhya Jyoti Konwar","doi":"10.1016/j.biortech.2025.132977","DOIUrl":"10.1016/j.biortech.2025.132977","url":null,"abstract":"<div><div>Herein, we report hydrolysis of crude ulvan sourced from <em>Ulva fasciata</em> using active carbon embedded sulfated resins as efficient catalysts affording high yield commercially important rare sugar <span>l</span>-rhamnose (upto 85 %) under mild conditions (90–120 °C, 8–24 h). These low-cost (hybrid) solid acid catalysts could be obtained from commercial grade macroporous sulfated resins and waste derived activated carbons by simple mechanochemical approach. Most importantly these catalysts outperformed the benchmark sulfated resins, HCl, solution mixtures of acids and carbon, and enzymes reported in prior art in terms of activity, monomeric sugar selectivity, and reusability (retained 90 % activity upto 5 cycles with the possibility of regeneration). Most importantly the use of such catalyst also afforded a high-quality hydrolysate free from furanic and oligomer impurities which enabled downstream <span>l</span>-rhamnose recovery by crystallization as <span>l</span>-rhamnose hydrate. A preliminary techno-economic analysis also indicated feasibility of a ulvan based <span>l</span>-rhamnose process with the developed catalysts.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132977"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gasification performance evaluation of soaked and dried bamboo feedstock in open downdraft gasifier 开放式下吸式气化炉中浸泡干燥竹原料的气化性能评价

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132976

Novandri Tri Setioputro , Muchammad , Eflita Yohana , Muhammad Fahd Fachrizal , Hermawan Dwi Ariyanto , Muhtar Kosim

{"title":"Gasification performance evaluation of soaked and dried bamboo feedstock in open downdraft gasifier","authors":"Novandri Tri Setioputro , Muchammad , Eflita Yohana , Muhammad Fahd Fachrizal , Hermawan Dwi Ariyanto , Muhtar Kosim","doi":"10.1016/j.biortech.2025.132976","DOIUrl":"10.1016/j.biortech.2025.132976","url":null,"abstract":"<div><div>The depletion of fossil fuels and the intensifying threat of climate change necessitate a transition toward renewable energy sources. Bamboo emerges as a promising biomass resource due to its rapid growth—approximately five times faster than hardwood. Through gasification, bamboo can be converted into synthetic gas (syngas), a combustible fuel. Pre-treatment by soaking significantly enhances bamboo’s gasification performance compared to drying. Soaked bamboo supports a stable gasification temperature and maintains optimal oxidation zone conditions, while dry bamboo increases the risk of charcoal buildup and system failure. Additionally, soaked bamboo achieves a syngas energy output improvement of 19.54 % and supports heat exchanger effectiveness up to 98 %. The resulting syngas also meets the tar content standards required for internal combustion engine applications, a criterion unmet by syngas from dry bamboo. These findings indicate that soaked bamboo is a viable and efficient renewable energy source for sustainable energy production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132976"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust partial nitrification and anammox under low-strength nitrogen condition by regulating organic-induced symbiosis of denitrifiers and anammox bacteria 通过调节有机诱导的反硝化菌与厌氧氨氧化菌的共生关系，在低强度氮条件下实现部分硝化和厌氧氨氧化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132975

Yulei Chi , Wuang Ren , Pengkang Jin , Xuan Shi , Lan Liu

{"title":"Robust partial nitrification and anammox under low-strength nitrogen condition by regulating organic-induced symbiosis of denitrifiers and anammox bacteria","authors":"Yulei Chi , Wuang Ren , Pengkang Jin , Xuan Shi , Lan Liu","doi":"10.1016/j.biortech.2025.132975","DOIUrl":"10.1016/j.biortech.2025.132975","url":null,"abstract":"<div><div>Low-strength nitrogen usually limits the efficiency of partial nitrification and anaerobic ammonium oxidation (anammox). To address this challenge, an organic-induced regulation strategy based on microbial interaction characteristics was proposed. Short-range addition of acetate for bio-screening is essential for achieving this regulation. After bio-screening, enhanced partial nitrification and anammox performance were achieved within 47 days. In the start-up phase, denitrifiers outcompeted nitrite oxidizing bacteria (NOB) for nitrite utilization, thereby enhancing the inhibition of NOB. The relative abundance of anammox bacteria (AnAOB) was maintained at approximately 8.6 % due to an increase in extracellular proteins, which was conducive to material exchange and symbiosis between denitrifiers and AnAOB. Transcriptomic analysis revealed that bio-screening induced mixotrophic metabolism of AnAOB, characterized by up-regulating the expression of genes involved in organic carbon and energy metabolism, thereby improving the stability of the anammox process. This study provides valuable insights for enhancing anammox performance under low-strength nitrogen conditions.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132975"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization techniques for biomass waste in energy Generation: A systematic review 生物质废弃物在能源生产中的增值技术：系统综述

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132973

Benjamin Yennuna Konyannik, Jerome Dela Lavie

{"title":"Valorization techniques for biomass waste in energy Generation: A systematic review","authors":"Benjamin Yennuna Konyannik, Jerome Dela Lavie","doi":"10.1016/j.biortech.2025.132973","DOIUrl":"10.1016/j.biortech.2025.132973","url":null,"abstract":"<div><div>The valorization of biomass waste into energy through thermal (i.e., gasification, pyrolysis), biological (i.e., anaerobic digestion) processes, and hybrid systems has garnered substantial attention due to its potential environmental, economic, and energy efficiency benefits. This systematic review aimed to provide a comprehensive analysis of various biomass waste valorization techniques for energy generation. Systematic searching in various databases and four key organizational websites yielded 317 relevant studies, of which 78 were selected according to the inclusion criteria. The review reveals the promise of techniques such as pyrolysis, gasification, anaerobic digestion, and hybrid systems for energy generation from biomass waste, while also emphasizing the influence of factors such as feedstock composition, process conditions, and energy conversion efficiency on their effectiveness. The capital investment requirement and payback period are presented in a detailed cost analysis as differing widely, from $3 million for fermentation to $10 million for incineration, with payback periods ranging from 4 to 8 years. The critique delineates technical challenges, such as feedstock heterogeneity and process optimization, and emphasizes the need for development in pre-processing, technological improvement, and integration with current energy infrastructures. The conclusions offer insightful information on the process efficiency, energy yield, and environmental sustainability of biomass valorization processes, thereby informing further research, policy-making, and industrial applications in sustainable energy generation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132973"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient de novo synthesis of 3-fucosyllactose in Escherichia coli via enzyme engineering and pathway refinement 利用酶工程和途径改进在大肠杆菌中高效地重新合成3-焦酰基乳糖

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132974

Ruoyu Jia , Mengli Li , Ming Miao , Tao Zhang

{"title":"Efficient de novo synthesis of 3-fucosyllactose in Escherichia coli via enzyme engineering and pathway refinement","authors":"Ruoyu Jia , Mengli Li , Ming Miao , Tao Zhang","doi":"10.1016/j.biortech.2025.132974","DOIUrl":"10.1016/j.biortech.2025.132974","url":null,"abstract":"<div><div>3-Fucosyllactose (3-FL), a functional oligosaccharide in human milk, holds great promise due to its health benefits. However, its microbial production is limited by the low activity of α-1,3-fucosyltransferase and the limited GDP-<span>l</span>-fucose supply. This study combined structure-based modeling and evolutionary analysis to identify five double-site mutants with enhanced 3-FL production. When the best-performing mutant N199V/K301P was expressed in double-copy, the 3-FL titer increased by 35 % (4.92 g/L) compared to the wild-type (3.65 g/L). To improve carbon flux and GDP-<span>l</span>-fucose supply, the engineered <em>Escherichia coli</em> strain L (BL21(DE3)Δ<em>lacZ</em>Δ<em>wcaJ</em>Δ<em>nudD</em>Δ<em>lon</em>) was further modified by deleting <em>mtlD</em>, <em>wcaE</em>, and <em>wcaI</em>—the latter two functionally validated for the first time in 3-FL biosynthesis. Through the synergistic optimization of these strategies, the best strains <span>l</span>-MEI carrying double-copy S98R/D340E or N199V/K301P achieved 3-FL titers of 53.88 g/L and 54.64 g/L in 3 L fed-batch fermentations, which are at relatively high levels among currently reported titers.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132974"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar enhanced floating root mats to reduce recalcitrant contaminants of emerging concern from wastewater effluents 生物炭增强漂浮根垫，以减少废水中出现的顽固污染物

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132960

Clara Laguna-Marín, Mònica Escolà Casas, Jèssica Subirats, Víctor Matamoros

{"title":"Biochar enhanced floating root mats to reduce recalcitrant contaminants of emerging concern from wastewater effluents","authors":"Clara Laguna-Marín, Mònica Escolà Casas, Jèssica Subirats, Víctor Matamoros","doi":"10.1016/j.biortech.2025.132960","DOIUrl":"10.1016/j.biortech.2025.132960","url":null,"abstract":"<div><div>This bench-scale study assessed a novel biochar-intensified floating root mat (FRM) system to reduce contaminants of emerging concern (CEC) from secondary-treated wastewater. Three configurations were evaluated: (1) FRM with plants (<em>Cyperus alternifolius</em>) and biochar, (2) FRM with plants only, and (3) unplanted control reactors, under two hydraulic loading rates (12 and 24 mm/day) over six months. The planted FRM enhanced CEC removal by 15 and 28 %, while the addition of biochar further increased removal by 35 % and 41 %, achieving CEC attenuation ranging from 41 to 99 %. Untargeted analysis corroborated this performance, and microbiological analysis linked CEC reduction to increased abundance of genera such as <em>Exiguobacterium, Nocardia, Rodobacter</em> and <em>Amaricoccus,</em> associated with biodegradation processes. This work therefore demonstrates that integrating biochar into FRMs enhances the attenuation of CEC and, for the first time, links biochar addition to microbial community shifts, offering a new strategy to tackle CEC pollution in wastewater treatment plants.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132960"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling and optimization of bioproduct formation with purple phototrophic bacteria using machine learning 利用机器学习对紫色光养细菌的生物产物生成进行建模和优化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132963

Germán Buitrón , Torsten Meyer , Elizabeth A. Edwards , Virginia Montiel-Corona

{"title":"Modeling and optimization of bioproduct formation with purple phototrophic bacteria using machine learning","authors":"Germán Buitrón , Torsten Meyer , Elizabeth A. Edwards , Virginia Montiel-Corona","doi":"10.1016/j.biortech.2025.132963","DOIUrl":"10.1016/j.biortech.2025.132963","url":null,"abstract":"<div><div>Municipal and industrial wastewater, along with organic waste, can be transformed into valuable bioproducts using purple phototrophic bacteria. This study compares the performance of three machine learning models (Random Forest, XGBoost, and CatBoost) in predicting and optimizing the formation of key bioproducts: polyhydroxybutyrate, polyhydroxyvalerate, 5-aminolevulinic acid, coenzyme Q10, carotenoids, bacteriochlorophylls, and biomass. The models were trained on a dataset compiled from previous studies, using input variables such as reaction time, concentration of organic matter, ethanol, bicarbonate, levulinic acid, ferric citrate, mineral medium, and N, C/N ratio, illumination conditions (continuous or intermittent), operation mode (batch or semicontinuous), and volume exchange percentage. Bayesian optimization was applied to train and tune the models. Performance was assessed using R<sup>2</sup>, Pearson correlation, RMSE, and MAPE. CatBoost outperformed the others, showing higher predictive correlation and lower error. It was subsequently used for further optimization. Feature importance analysis identified reaction time, mineral medium concentration, and volume exchange percentage as key drivers of bioproduct synthesis. The Particle Swarm Optimization algorithm was applied to determine optimal conditions for each target compound. Under the conditions studied, predicted maximum yields were: 569 mg polyhydroxybutyrate/L, 45 mg polyhydroxyvalerate/L, 79 µmol 5-aminolevulinic acid/L, 13 mg coenzyme Q10/g dw, 7 mg carotenoids/g dw, 17 mg bacteriochlorophylls/g dw, and 2040 mg biomass/L. Optimization suggests that operating as a sequencing batch reactor and employing discontinuous illumination for most targets, along with a reduced mineral medium concentration, is beneficial. Results highlight that each bioproduct requires distinct operational settings, supporting the idea of clustering target compounds.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132963"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0