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Enzymatic processes for animal hide/skin collagen fiber purification processing: Recent progress, challenges and recommendations. 动物皮/皮肤胶原纤维纯化加工的酶促工艺:最新进展、挑战和建议。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI: 10.1016/j.biortech.2024.131955
Xu Zhang, Mengchu Gao, Chunxiao Zhang, Biyu Peng
{"title":"Enzymatic processes for animal hide/skin collagen fiber purification processing: Recent progress, challenges and recommendations.","authors":"Xu Zhang, Mengchu Gao, Chunxiao Zhang, Biyu Peng","doi":"10.1016/j.biortech.2024.131955","DOIUrl":"10.1016/j.biortech.2024.131955","url":null,"abstract":"<p><p>Collagen fiber purification is the most important pretreatment process in the recycling of animal hide/skin, by-products of meat production, and can be utilized to produce value-added materials. Traditional animal hide/skin resource utilization technologies face serious challenges in the aspect of production efficiency and environmental sustainability. Enzymatic collagen fiber purification processing is thought to be one of the most promising technologies that can minimize the use of chemicals and energy, reduce CO<sub>2</sub>-eq emissions, and achieve sustainable development of animal hide/skin reutilization. However, enzymatic processes have not been well accepted for industrial-scale applications in factories so far. In this review, recent progress and challenges of enzymatic collagen fiber purification processing were comprehensively overviewed in the aspect of the key mechanisms and technologies of enzyme application. Recommendations for the direction of enzyme selection and development were put forward, which is expected to pave the way for the industrial-scale application of enzymes in animal hide/skin collagen fiber purification processing.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131955"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Critical review on on-site sanitation technologies: Typologies, treatment and transition towards circular economy. 现场卫生技术综述:类型、处理和向循环经济过渡。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-07 DOI: 10.1016/j.biortech.2024.131954
Aakash Patwa, Amit Kumar, Ritesh Vijay
{"title":"Critical review on on-site sanitation technologies: Typologies, treatment and transition towards circular economy.","authors":"Aakash Patwa, Amit Kumar, Ritesh Vijay","doi":"10.1016/j.biortech.2024.131954","DOIUrl":"10.1016/j.biortech.2024.131954","url":null,"abstract":"<p><p>Around 4.2 billion people globally depend on on-site sanitation systems, with 43% relying on basic or unsafe facilities with key challenges of containment, emptying, transport, treatment and resource recovery from faecal sludge. This review paper critically examines faecal sludge characteristics and treatment technologies in terms of urine diversion capability, land requirements and capital as well as operational expenditure based on Indian and international practices. Apart from these, the review also provides reuse-recycle of processed manure, treated effluent and bio-energy from faecal sludge to create environmental and economic benefits. The review also highlights future research on the development of nature-based solutions for solid-liquid separation and treatment, low footprints and on-site treatment for fostering a circular economy. Ultimately this review emphasizes the importance of advancing faecal sludge management to address global sanitation challenges and to create sustainable solutions for environmental protection.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131954"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of degradable and non-degradable microplastics on SPNEDPR-AGS system: Sludge characteristics, nutrient transformation, key enzyme, and microbial community. 可降解和不可降解微塑料对spnedprr - ags系统的影响:污泥特性、营养转化、关键酶和微生物群落
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-11-30 DOI: 10.1016/j.biortech.2024.131917
Dongyue Li, Jiarui Li, Dongbo Liang, Yanshuo Wu, Chaofan Xie, Muchen Yin, Yuhan Zhu, Yaodong Wu, Linzhu Du, Junhui Yue, Jun Li, Wei Guo
{"title":"Effects of degradable and non-degradable microplastics on SPNEDPR-AGS system: Sludge characteristics, nutrient transformation, key enzyme, and microbial community.","authors":"Dongyue Li, Jiarui Li, Dongbo Liang, Yanshuo Wu, Chaofan Xie, Muchen Yin, Yuhan Zhu, Yaodong Wu, Linzhu Du, Junhui Yue, Jun Li, Wei Guo","doi":"10.1016/j.biortech.2024.131917","DOIUrl":"10.1016/j.biortech.2024.131917","url":null,"abstract":"<p><p>The environmental risk of microplastics (MPs) in aerobic granular sludge (AGS) system is unclear. This study evaluates the effects of non-biodegradable polyvinyl chloride microplastics (PVC-MPs) and biodegradable polylactic acid microplastics (PLA-MPs) on AGS systems. The results showed that both destroyed the performance of AGS systems, with PVC-MPs achieving this by disrupting the AGS structure, while PLA-MPs mainly by causing the expansion of filamentous bacteria induced through the stimulation by lactic acid metabolite (R0: 5.52 ± 0.49 μg/L; R<sub>PLA</sub>5: 11.67 ± 0.56 μg/L). Moreover, both MPs inhibited nitrogen removal by disrupting partial nitrification and endogenous denitrification and suppressed key microbes such as Candidatus Competibacter and Nitrosomonas. Metabolic pathway analysis and molecular docking have further confirmed the mechanisms by which MPs affect critical metabolic pathways and key enzymes. Consequently, the hazards of biodegradable MPs to the stable operation of sewage treatment plants should also be of concern.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131917"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements. 逐步处理小球藻可以提供植物生物刺激素,减少对矿物肥料的需求。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-01 DOI: 10.1016/j.biortech.2024.131923
Erik Chovancek, Sylvain Poque, Engin Bayram, Emren Borhan, Martina Jokel, Iida-Maria Rantanen, Berat Z Haznedaroglu, Kristiina Himanen, Sema Sirin, Yagut Allahverdiyeva
{"title":"Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements.","authors":"Erik Chovancek, Sylvain Poque, Engin Bayram, Emren Borhan, Martina Jokel, Iida-Maria Rantanen, Berat Z Haznedaroglu, Kristiina Himanen, Sema Sirin, Yagut Allahverdiyeva","doi":"10.1016/j.biortech.2024.131923","DOIUrl":"10.1016/j.biortech.2024.131923","url":null,"abstract":"<p><p>We developed a stepwise method to transform Chlorella sorokiniana microalgal biomass into a potent biostimulant. The method, including maceration, high-pressure homogenization, and enzymatic hydrolysis, preserves the bioactive properties of the biomass as a biostimulant while minimizing plant inhibitory effects. Fractions were characterized individually, and optimal concentrations were determined using a rapid Arabidopsis root assay. A blend of optimal concentrations of fractions was identified as the most stimulating extract, increasing the root elongation by 25 %. When applied to tomato plants and monitored using high-throughput plant phenotyping, the blend displayed a 25 % reduction in mineral fertilizer use. Metabolomic analysis of the tomato plants showed significantly enhanced carbon and nitrogen metabolism in the leaves. Our findings indicate that the stepwise processing not only produces an effective biostimulant but also generates substantial residual biomass for a potential multiproduct biorefinery approach that can improve the overall techno-economic outlook.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131923"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insight into enhanced enrichment and nitrogen removal performance of Anammox bacteria with novel biochar/tourmaline polyurethane sponge modified biocarrier. 新型生物炭/电气石聚氨酯海绵改性生物载体对厌氧氨氧化菌富集和脱氮性能的影响
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-04 DOI: 10.1016/j.biortech.2024.131946
Yanchen Li, Wenyi Dong, Zilong Hou, Huaguang Liu, Kaiyuan Shi, Shuo Chen, Hongjie Wang
{"title":"Insight into enhanced enrichment and nitrogen removal performance of Anammox bacteria with novel biochar/tourmaline polyurethane sponge modified biocarrier.","authors":"Yanchen Li, Wenyi Dong, Zilong Hou, Huaguang Liu, Kaiyuan Shi, Shuo Chen, Hongjie Wang","doi":"10.1016/j.biortech.2024.131946","DOIUrl":"10.1016/j.biortech.2024.131946","url":null,"abstract":"<p><p>A novel biochar/tourmaline polyurethane sponge modified biocarrier (BTP) could enhance Anammox bacteria (AnAOB) enrichment and nitrogen removal performance. With higher hydrophilicity and specific surface area, BTP significantly improved total inorganic nitrogen (TIN) removal efficiency to 80 ± 2 %, compared to unmodified biocarrier of 67 ± 3 % when influent TIN reached 633.9 ± 22.0 mg/L. BTP stimulated the upregulation of amino acid synthases genes abundance and improved protein secretion in extracellular polymer substances (EPS). Moreover, significant increases were found in heme concentration, specific anammox activity and hydrazine dehydrogenase of AnAOB with BTP compared to unmodified biocarrier. Extracellular electron transfer pathway of AnAOB was improved by BTP via upregulating cytochrome C and ferredoxin synthesis. Candidatus Brocadia was the main genus in Anammox biofilm, with relative abundance of 20.1 % and 27.6 % in the control and BTP, respectively, which explained the improvement of nitrogen removal performance.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131946"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comprehensive review of antibiotics stress on anammox systems: Mechanisms, applications, and challenges. 抗生素对厌氧氨氧化系统的影响:机制、应用和挑战。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-06 DOI: 10.1016/j.biortech.2024.131950
Zhimin He, Gongduan Fan, Zongqiong Xu, Shiyun Wu, Jiankun Xie, Wei Qiang, Kai-Qin Xu
{"title":"A comprehensive review of antibiotics stress on anammox systems: Mechanisms, applications, and challenges.","authors":"Zhimin He, Gongduan Fan, Zongqiong Xu, Shiyun Wu, Jiankun Xie, Wei Qiang, Kai-Qin Xu","doi":"10.1016/j.biortech.2024.131950","DOIUrl":"10.1016/j.biortech.2024.131950","url":null,"abstract":"<p><p>Anaerobic ammonia oxidation (anammox), an energy-efficient technology for treating ammonium-rich wastewater, faces the challenge of antibiotic stress in sewage. This paper systematically evaluated the impact of antibiotics on anammox by considering both inhibitory effects and recovery duration. This review focused on cellular responses, including extracellular polymeric substances (EPS), quorum sensing (QS), and enzymes. Then, the physiological properties of cells and the interactions between nitrogen and carbon metabolism under antibiotic stress were discussed, particularly within the anammoxosome. The microbial community evolution and the development and transfer of antibiotic resistance genes (ARGs) were further analyzed to reveal the resistance mechanisms of anammox. To address the limitations imposed by antibiotics, the development of bio-augmentation and combined processes based on molecular biology techniques, such as bio-electrochemical systems (BES), has been suggested. This review offered new insights into the mechanisms of antibiotic inhibition during the anammox process and aimed to advance their engineering applications.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131950"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermophilic bacteria contributing to humus accumulation in hyperthermophilic aerobic fermentation of mushroom residue. 嗜热菌对蘑菇渣超嗜热好氧发酵腐殖质积累的贡献。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-06 DOI: 10.1016/j.biortech.2024.131957
Xiaoqin Zhou, Zhen Yu, Kaipeng Zhai, Wenkang Deng, Li Zhuang, Yueqiang Wang, Qiang Zhang, Shungui Zhou
{"title":"Thermophilic bacteria contributing to humus accumulation in hyperthermophilic aerobic fermentation of mushroom residue.","authors":"Xiaoqin Zhou, Zhen Yu, Kaipeng Zhai, Wenkang Deng, Li Zhuang, Yueqiang Wang, Qiang Zhang, Shungui Zhou","doi":"10.1016/j.biortech.2024.131957","DOIUrl":"10.1016/j.biortech.2024.131957","url":null,"abstract":"<p><p>The purpose of this study is to clarify the roles of thermophilic bacteria in humification during hyperthermophilic composting (HTC) of organic wastes mainly composed of mushroom residue. Results showed that HTC with a long hyperthermophilic (>80°C) period lasting for 18 days produced 83 mg/g of humus in compost on day 27, significantly higher than that of thermophilic composting (TC, 9.7 mg/g). Machine learning models identified that the dominant thermophiles belonging to Bacillaceae, Sporolactobacillaceae, Thermaerobacteraceae, Paenibacillaceae families and the unique thermophiles (Thermus and Calditerricola) in HTC played important roles in accumulating stubborn and soluble humus including humic acid and fulvic acid. Hyperthermophilic fermentation not only recruited and enriched these thermophilic bacteria to rapidly degrade organic matter into bioavailable nutrients, but also upregulated the metabolic pathways relevant to the generation and oxidation of precursors including amino acids that would be polymerized into humus, thus efficiently converting organic waste into humus-rich compost.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131957"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microalgae - bacteria based wastewater treatment systems: Granulation, influence factors and pollutants removal. 微藻-细菌废水处理系统:造粒、影响因素和污染物去除。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.biortech.2024.131973
Mai-Duy-Thong Pham, Xuan-Thanh Bui, Thi-Kim-Quyen Vo, Thanh-Son Dao, Linh-Thy Le, Thi-Dieu-Hien Vo, Ky-Phuong-Ha Huynh, Thanh-Binh Nguyen, Chitsan Lin, Chettiyappan Visvanathan
{"title":"Microalgae - bacteria based wastewater treatment systems: Granulation, influence factors and pollutants removal.","authors":"Mai-Duy-Thong Pham, Xuan-Thanh Bui, Thi-Kim-Quyen Vo, Thanh-Son Dao, Linh-Thy Le, Thi-Dieu-Hien Vo, Ky-Phuong-Ha Huynh, Thanh-Binh Nguyen, Chitsan Lin, Chettiyappan Visvanathan","doi":"10.1016/j.biortech.2024.131973","DOIUrl":"10.1016/j.biortech.2024.131973","url":null,"abstract":"<p><p>Wastewater treatment based on microalgae and bacteria symbiosis is an environmentally friendly, sustainable technology that has attracted attention recently because of its high efficiency in treating pollutants, saving energy, and short-term biomass recovery. Among them, the granular microalgae and bacteria combination emerges with the advantages of rapid gravity settling, good resistance to adverse environmental conditions, outstanding wastewater treatment performance, and easy biomass recovery. This review aims to clarify the microalgal-bacterial granule (MBG) - based process for wastewater treatment. In particular, MBG characteristics, granulation mechanism, and influence factors on the process are also discussed. The review contributes to the knowledge system related to MBG research in recent years, thereby pointing out research gaps that need to be filled in the future.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131973"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancement of dewatering performance and effective degradation of petroleum hydrocarbons in biological oily sludge using atmospheric pressure plasma jet. 常压等离子体射流提高生物含油污泥脱水性能及有效降解石油烃。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-12 DOI: 10.1016/j.biortech.2024.131974
Pengcheng Xia, Siyi Jing, Zhenqing Zhao, Tongtong Dou, Jie Gao, Ziyi Guo, Lijie Xu, He Guo, Ming Zhang, Weichuan Qiao
{"title":"Enhancement of dewatering performance and effective degradation of petroleum hydrocarbons in biological oily sludge using atmospheric pressure plasma jet.","authors":"Pengcheng Xia, Siyi Jing, Zhenqing Zhao, Tongtong Dou, Jie Gao, Ziyi Guo, Lijie Xu, He Guo, Ming Zhang, Weichuan Qiao","doi":"10.1016/j.biortech.2024.131974","DOIUrl":"10.1016/j.biortech.2024.131974","url":null,"abstract":"<p><p>The presence of petroleum hydrocarbon components (PHCs) in biological oily sludge increases the toxicity of the sludge and makes dewatering even more difficult. In this study, an atmospheric pressure plasma jet (APPJ) technology was used for treating biological oily sludge. The results showed that under specific conditions-a sludge/water ratio of 1:100, a discharge power of 440 W, and a 60-min treatment-the degradation rate of PHCs reached 36.3 %. APPJ can convert larger molecule PHCs and extracellular polymeric substances, thereby reducing the particle size and viscosity of the sludge. Meanwhile, the sludge volume was reduced and the sludge dewatering efficiency was improved. The hydroxyl radical and superoxide radical generated by APPJ were the main active species involved in the degradation process. This work provides a novel strategy for the reduction of biological oily sludge and the synergistic removal of PHCs along with the sludge.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131974"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous enhancement of activity and stability of Bacillus safensis-derived laccase and its application in lignocellulose saccharification. 同时增强萨氏芽孢杆菌源漆酶的活性和稳定性及其在木质纤维素糖化中的应用。
IF 9.7 1区 环境科学与生态学
Bioresource Technology Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI: 10.1016/j.biortech.2024.131983
Wenhua Yang, Xiangyang Ma, Hui Sun, Jiahui Wang, Jiyan Li, Xiuxiu Chu, Jianli Zhou, Fuping Lu, Yihan Liu
{"title":"Simultaneous enhancement of activity and stability of Bacillus safensis-derived laccase and its application in lignocellulose saccharification.","authors":"Wenhua Yang, Xiangyang Ma, Hui Sun, Jiahui Wang, Jiyan Li, Xiuxiu Chu, Jianli Zhou, Fuping Lu, Yihan Liu","doi":"10.1016/j.biortech.2024.131983","DOIUrl":"10.1016/j.biortech.2024.131983","url":null,"abstract":"<p><p>Effective hydrolysis of lignocelluloses for producing reducing sugar is impeded by the covalent binding of hemicellulose and cellulose through lignin, which could be eliminated by laccases. This study identified a novel thermostable laccase from Bacillus safensis TCCC 111022 and created an iterative mutant E231D/Y441H, exhibiting 1.59-fold greater specific activity and a 183 % greater half-life at 80°C than the wild-type enzyme. Computational analysis revealed that the stability and activity of the E231D/Y441H could be simultaneously enhanced by increasing the flexibility of the ring around the substrate binding pocket. Additionally, the saccharification efficiency of sugarcane bagasse and corn stalks were both enhanced by 235 % in the system adding E231D/Y441H, mixed-cellulases, and mediator (1-hydroxybenzotriazole) compared to the samples treated with mixed-cellulases. The findings of this research provide a reference for the degradation of lignocellulosic substrates and contribute to the sustainable development of biomass-based industries.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131983"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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