Bioresource Technology最新文献_第4页

Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting 微生物介导的Fenton系统对秸秆堆肥过程中木质素转化的协同强化机制

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132981

Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei

{"title":"Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting","authors":"Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei","doi":"10.1016/j.biortech.2025.132981","DOIUrl":"10.1016/j.biortech.2025.132981","url":null,"abstract":"<div><div>This study investigated the synergistic strengthening mechanism of a microbial-mediated Fenton system in lignin depolymerization during composting. The Fenton system was constructed using hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced by lignin-degrading microorganisms along with Fe(Ⅱ). The simultaneous inoculation of bacteria and fungi resulted in optimal enzyme activities related to lignocellulose degradation and lignin degradation rates. Moreover, the addition of 0.5% FeSO<sub>4</sub> further optimized hydroxyl radical (<strong>·</strong>OH) production. Therefore, four treatments were set: CK (control), BHF (bacterial fungi synchronized inoculation), Fe (FeSO<sub>4</sub>), and BHF-Fe (bacterial fungi synchronized inoculation + FeSO<sub>4</sub>). BHF-Fe synergistically drove the Fe(Ⅱ)/Fe(Ⅲ) redox cycle and maintained reactive oxygen species ROS, (superoxide anion, H<sub>2</sub>O<sub>2</sub> and <strong>·</strong>OH) generation. BHF-Fe showed a 22.58% greater lignin loss rate compared to the CK. The correlations between ROS and lignin loss rate were enhanced. Additionally, lignin peroxidase, as an extracellular enzyme, relies on H<sub>2</sub>O<sub>2</sub> for its action on lignin. The activities of laccase and lignin peroxidase were significantly correlated. Lignin decomposition was accelerated through the upregulation of key degrading enzyme genes (<em>Lac</em>, <em>LiP</em>, and <em>MnP</em>). The Fenton system showed attenuated pH dependence relative to conventional systems despite pH and Fe (II) effects on ROS. The BHF-Fe achieves efficient lignin degradation through a synergistic mechanism that integrates Fenton reactions, ROS, biocatalytic enzymatic oxidation and microenvironment compensation. This research offers theoretical and technical backing for developing agricultural waste resource recycling and composting technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132981"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient production of l-rhamnose from catalytic hydrolysis of marine polysaccharide ulvan over carbon embedded sulfonated resins 碳包埋磺化树脂催化水解海洋多糖ulvan高效生产l-鼠李糖

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132977

Milan Arvindbhai Dumraliya , Satish Kumar Nayak , R. Suhail Haq , Sanket Nareshbhai Balar , Nikhilesh Trivedi , Mariappan Mani , S. Dinesh Kumar , V. Veeragurunathan , Lakhya Jyoti Konwar

{"title":"Highly efficient production of l-rhamnose from catalytic hydrolysis of marine polysaccharide ulvan over carbon embedded sulfonated resins","authors":"Milan Arvindbhai Dumraliya , Satish Kumar Nayak , R. Suhail Haq , Sanket Nareshbhai Balar , Nikhilesh Trivedi , Mariappan Mani , S. Dinesh Kumar , V. Veeragurunathan , Lakhya Jyoti Konwar","doi":"10.1016/j.biortech.2025.132977","DOIUrl":"10.1016/j.biortech.2025.132977","url":null,"abstract":"<div><div>Herein, we report hydrolysis of crude ulvan sourced from <em>Ulva fasciata</em> using active carbon embedded sulfated resins as efficient catalysts affording high yield commercially important rare sugar <span>l</span>-rhamnose (upto 85 %) under mild conditions (90–120 °C, 8–24 h). These low-cost (hybrid) solid acid catalysts could be obtained from commercial grade macroporous sulfated resins and waste derived activated carbons by simple mechanochemical approach. Most importantly these catalysts outperformed the benchmark sulfated resins, HCl, solution mixtures of acids and carbon, and enzymes reported in prior art in terms of activity, monomeric sugar selectivity, and reusability (retained 90 % activity upto 5 cycles with the possibility of regeneration). Most importantly the use of such catalyst also afforded a high-quality hydrolysate free from furanic and oligomer impurities which enabled downstream <span>l</span>-rhamnose recovery by crystallization as <span>l</span>-rhamnose hydrate. A preliminary techno-economic analysis also indicated feasibility of a ulvan based <span>l</span>-rhamnose process with the developed catalysts.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132977"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gasification performance evaluation of soaked and dried bamboo feedstock in open downdraft gasifier 开放式下吸式气化炉中浸泡干燥竹原料的气化性能评价

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132976

Novandri Tri Setioputro , Muchammad , Eflita Yohana , Muhammad Fahd Fachrizal , Hermawan Dwi Ariyanto , Muhtar Kosim

{"title":"Gasification performance evaluation of soaked and dried bamboo feedstock in open downdraft gasifier","authors":"Novandri Tri Setioputro , Muchammad , Eflita Yohana , Muhammad Fahd Fachrizal , Hermawan Dwi Ariyanto , Muhtar Kosim","doi":"10.1016/j.biortech.2025.132976","DOIUrl":"10.1016/j.biortech.2025.132976","url":null,"abstract":"<div><div>The depletion of fossil fuels and the intensifying threat of climate change necessitate a transition toward renewable energy sources. Bamboo emerges as a promising biomass resource due to its rapid growth—approximately five times faster than hardwood. Through gasification, bamboo can be converted into synthetic gas (syngas), a combustible fuel. Pre-treatment by soaking significantly enhances bamboo’s gasification performance compared to drying. Soaked bamboo supports a stable gasification temperature and maintains optimal oxidation zone conditions, while dry bamboo increases the risk of charcoal buildup and system failure. Additionally, soaked bamboo achieves a syngas energy output improvement of 19.54 % and supports heat exchanger effectiveness up to 98 %. The resulting syngas also meets the tar content standards required for internal combustion engine applications, a criterion unmet by syngas from dry bamboo. These findings indicate that soaked bamboo is a viable and efficient renewable energy source for sustainable energy production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132976"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust partial nitrification and anammox under low-strength nitrogen condition by regulating organic-induced symbiosis of denitrifiers and anammox bacteria 通过调节有机诱导的反硝化菌与厌氧氨氧化菌的共生关系，在低强度氮条件下实现部分硝化和厌氧氨氧化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132975

Yulei Chi , Wuang Ren , Pengkang Jin , Xuan Shi , Lan Liu

{"title":"Robust partial nitrification and anammox under low-strength nitrogen condition by regulating organic-induced symbiosis of denitrifiers and anammox bacteria","authors":"Yulei Chi , Wuang Ren , Pengkang Jin , Xuan Shi , Lan Liu","doi":"10.1016/j.biortech.2025.132975","DOIUrl":"10.1016/j.biortech.2025.132975","url":null,"abstract":"<div><div>Low-strength nitrogen usually limits the efficiency of partial nitrification and anaerobic ammonium oxidation (anammox). To address this challenge, an organic-induced regulation strategy based on microbial interaction characteristics was proposed. Short-range addition of acetate for bio-screening is essential for achieving this regulation. After bio-screening, enhanced partial nitrification and anammox performance were achieved within 47 days. In the start-up phase, denitrifiers outcompeted nitrite oxidizing bacteria (NOB) for nitrite utilization, thereby enhancing the inhibition of NOB. The relative abundance of anammox bacteria (AnAOB) was maintained at approximately 8.6 % due to an increase in extracellular proteins, which was conducive to material exchange and symbiosis between denitrifiers and AnAOB. Transcriptomic analysis revealed that bio-screening induced mixotrophic metabolism of AnAOB, characterized by up-regulating the expression of genes involved in organic carbon and energy metabolism, thereby improving the stability of the anammox process. This study provides valuable insights for enhancing anammox performance under low-strength nitrogen conditions.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132975"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization techniques for biomass waste in energy Generation: A systematic review 生物质废弃物在能源生产中的增值技术：系统综述

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132973

Benjamin Yennuna Konyannik, Jerome Dela Lavie

{"title":"Valorization techniques for biomass waste in energy Generation: A systematic review","authors":"Benjamin Yennuna Konyannik, Jerome Dela Lavie","doi":"10.1016/j.biortech.2025.132973","DOIUrl":"10.1016/j.biortech.2025.132973","url":null,"abstract":"<div><div>The valorization of biomass waste into energy through thermal (i.e., gasification, pyrolysis), biological (i.e., anaerobic digestion) processes, and hybrid systems has garnered substantial attention due to its potential environmental, economic, and energy efficiency benefits. This systematic review aimed to provide a comprehensive analysis of various biomass waste valorization techniques for energy generation. Systematic searching in various databases and four key organizational websites yielded 317 relevant studies, of which 78 were selected according to the inclusion criteria. The review reveals the promise of techniques such as pyrolysis, gasification, anaerobic digestion, and hybrid systems for energy generation from biomass waste, while also emphasizing the influence of factors such as feedstock composition, process conditions, and energy conversion efficiency on their effectiveness. The capital investment requirement and payback period are presented in a detailed cost analysis as differing widely, from $3 million for fermentation to $10 million for incineration, with payback periods ranging from 4 to 8 years. The critique delineates technical challenges, such as feedstock heterogeneity and process optimization, and emphasizes the need for development in pre-processing, technological improvement, and integration with current energy infrastructures. The conclusions offer insightful information on the process efficiency, energy yield, and environmental sustainability of biomass valorization processes, thereby informing further research, policy-making, and industrial applications in sustainable energy generation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"435 ","pages":"Article 132973"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient de novo synthesis of 3-fucosyllactose in Escherichia coli via enzyme engineering and pathway refinement 利用酶工程和途径改进在大肠杆菌中高效地重新合成3-焦酰基乳糖

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132974

Ruoyu Jia , Mengli Li , Ming Miao , Tao Zhang

{"title":"Efficient de novo synthesis of 3-fucosyllactose in Escherichia coli via enzyme engineering and pathway refinement","authors":"Ruoyu Jia , Mengli Li , Ming Miao , Tao Zhang","doi":"10.1016/j.biortech.2025.132974","DOIUrl":"10.1016/j.biortech.2025.132974","url":null,"abstract":"<div><div>3-Fucosyllactose (3-FL), a functional oligosaccharide in human milk, holds great promise due to its health benefits. However, its microbial production is limited by the low activity of α-1,3-fucosyltransferase and the limited GDP-<span>l</span>-fucose supply. This study combined structure-based modeling and evolutionary analysis to identify five double-site mutants with enhanced 3-FL production. When the best-performing mutant N199V/K301P was expressed in double-copy, the 3-FL titer increased by 35 % (4.92 g/L) compared to the wild-type (3.65 g/L). To improve carbon flux and GDP-<span>l</span>-fucose supply, the engineered <em>Escherichia coli</em> strain L (BL21(DE3)Δ<em>lacZ</em>Δ<em>wcaJ</em>Δ<em>nudD</em>Δ<em>lon</em>) was further modified by deleting <em>mtlD</em>, <em>wcaE</em>, and <em>wcaI</em>—the latter two functionally validated for the first time in 3-FL biosynthesis. Through the synergistic optimization of these strategies, the best strains <span>l</span>-MEI carrying double-copy S98R/D340E or N199V/K301P achieved 3-FL titers of 53.88 g/L and 54.64 g/L in 3 L fed-batch fermentations, which are at relatively high levels among currently reported titers.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132974"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar enhanced floating root mats to reduce recalcitrant contaminants of emerging concern from wastewater effluents 生物炭增强漂浮根垫，以减少废水中出现的顽固污染物

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132960

Clara Laguna-Marín, Mònica Escolà Casas, Jèssica Subirats, Víctor Matamoros

{"title":"Biochar enhanced floating root mats to reduce recalcitrant contaminants of emerging concern from wastewater effluents","authors":"Clara Laguna-Marín, Mònica Escolà Casas, Jèssica Subirats, Víctor Matamoros","doi":"10.1016/j.biortech.2025.132960","DOIUrl":"10.1016/j.biortech.2025.132960","url":null,"abstract":"<div><div>This bench-scale study assessed a novel biochar-intensified floating root mat (FRM) system to reduce contaminants of emerging concern (CEC) from secondary-treated wastewater. Three configurations were evaluated: (1) FRM with plants (<em>Cyperus alternifolius</em>) and biochar, (2) FRM with plants only, and (3) unplanted control reactors, under two hydraulic loading rates (12 and 24 mm/day) over six months. The planted FRM enhanced CEC removal by 15 and 28 %, while the addition of biochar further increased removal by 35 % and 41 %, achieving CEC attenuation ranging from 41 to 99 %. Untargeted analysis corroborated this performance, and microbiological analysis linked CEC reduction to increased abundance of genera such as <em>Exiguobacterium, Nocardia, Rodobacter</em> and <em>Amaricoccus,</em> associated with biodegradation processes. This work therefore demonstrates that integrating biochar into FRMs enhances the attenuation of CEC and, for the first time, links biochar addition to microbial community shifts, offering a new strategy to tackle CEC pollution in wastewater treatment plants.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132960"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling and optimization of bioproduct formation with purple phototrophic bacteria using machine learning 利用机器学习对紫色光养细菌的生物产物生成进行建模和优化

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-12 DOI: 10.1016/j.biortech.2025.132963

Germán Buitrón , Torsten Meyer , Elizabeth A. Edwards , Virginia Montiel-Corona

{"title":"Modeling and optimization of bioproduct formation with purple phototrophic bacteria using machine learning","authors":"Germán Buitrón , Torsten Meyer , Elizabeth A. Edwards , Virginia Montiel-Corona","doi":"10.1016/j.biortech.2025.132963","DOIUrl":"10.1016/j.biortech.2025.132963","url":null,"abstract":"<div><div>Municipal and industrial wastewater, along with organic waste, can be transformed into valuable bioproducts using purple phototrophic bacteria. This study compares the performance of three machine learning models (Random Forest, XGBoost, and CatBoost) in predicting and optimizing the formation of key bioproducts: polyhydroxybutyrate, polyhydroxyvalerate, 5-aminolevulinic acid, coenzyme Q10, carotenoids, bacteriochlorophylls, and biomass. The models were trained on a dataset compiled from previous studies, using input variables such as reaction time, concentration of organic matter, ethanol, bicarbonate, levulinic acid, ferric citrate, mineral medium, and N, C/N ratio, illumination conditions (continuous or intermittent), operation mode (batch or semicontinuous), and volume exchange percentage. Bayesian optimization was applied to train and tune the models. Performance was assessed using R<sup>2</sup>, Pearson correlation, RMSE, and MAPE. CatBoost outperformed the others, showing higher predictive correlation and lower error. It was subsequently used for further optimization. Feature importance analysis identified reaction time, mineral medium concentration, and volume exchange percentage as key drivers of bioproduct synthesis. The Particle Swarm Optimization algorithm was applied to determine optimal conditions for each target compound. Under the conditions studied, predicted maximum yields were: 569 mg polyhydroxybutyrate/L, 45 mg polyhydroxyvalerate/L, 79 µmol 5-aminolevulinic acid/L, 13 mg coenzyme Q10/g dw, 7 mg carotenoids/g dw, 17 mg bacteriochlorophylls/g dw, and 2040 mg biomass/L. Optimization suggests that operating as a sequencing batch reactor and employing discontinuous illumination for most targets, along with a reduced mineral medium concentration, is beneficial. Results highlight that each bioproduct requires distinct operational settings, supporting the idea of clustering target compounds.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132963"},"PeriodicalIF":9.7,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring lignin surface properties for enhanced enzymatic hydrolysis: Comparative insights from pine and eucalyptus 剪裁木质素表面性质增强酶水解：比较见解从松树和桉树

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132972

Yoon-Jung Shin , Jae-Won Lee

{"title":"Tailoring lignin surface properties for enhanced enzymatic hydrolysis: Comparative insights from pine and eucalyptus","authors":"Yoon-Jung Shin , Jae-Won Lee","doi":"10.1016/j.biortech.2025.132972","DOIUrl":"10.1016/j.biortech.2025.132972","url":null,"abstract":"<div><div>Milled wood lignin (MWL) was isolated from pine and eucalyptus before and after hydrothermal treatment, and its effects on enzymatic hydrolysis were evaluated. Hydrothermal treatment primarily decomposed hemicellulose and induced structural changes in lignin for both species. With increasing treatment severity, the hydrophobicity and lignin surface area decreased, the zeta potential became more negative, aliphatic hydroxyl group content decreased, and β-O-4 linkages were cleaved. These changes influenced enzymatic hydrolysis, with the highest glucan to glucose conversion observed in pretreated eucalyptus (HT60). Correlation analysis indicated that enzymatic hydrolysis efficiency in pine was most strongly influenced by cellulose content, phenolic hydroxyl group in lignin, and zeta potential, while in eucalyptus, the key factors were cellulose content, aliphatic hydroxyl group content, and zeta potential. The role of lignin hydroxyl group in enzyme adsorption was further examined through MWL acetylation, which demonstrated that acetylated MWL exhibited reduced enzyme adsorption compared to unmodified MWL.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132972"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Augmented methanogenic performance in straw-manure co-digestion via micro/nano bubble-enhanced syntrophic metabolism 微/纳米气泡增强合成代谢提高秸秆粪共消化产甲烷性能

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-11 DOI: 10.1016/j.biortech.2025.132955

Yanning Hou , Chao Liu , Jianhong Wei , Wenyan Zhao , Binghua Yan

{"title":"Augmented methanogenic performance in straw-manure co-digestion via micro/nano bubble-enhanced syntrophic metabolism","authors":"Yanning Hou , Chao Liu , Jianhong Wei , Wenyan Zhao , Binghua Yan","doi":"10.1016/j.biortech.2025.132955","DOIUrl":"10.1016/j.biortech.2025.132955","url":null,"abstract":"<div><div>This study aimed to investigate the enhancement of methane production by comparing air-nanobubble water (air-NBW) and conventional micro-bubble aeration during the anaerobic co-digestion (AcoD) of rice straw and pig manure. Remarkably, the addition of air-NBW (T3-NBW), as the most effective gas supplementation strategy, resulted in the highest cumulative methane yield of 489.49 mL/g·VS with a minimal lag phase of 0.57 days, representing a 59.80 % increase over the control (306.49 mL/g·VS, <em>p</em> < 0.001). This finding aligns with the kinetic fitting data (477.85 ± 5.84 mL/g·VS), wherein the maximum methane production rate for the T3-NBW was 41.89 mL/g∙VS·d<sup>−1</sup>. Mechanistic insights revealed that air-NBW dramatically elevated the activity of β-glucosidase (hydrolysis) and coenzyme F<sub>420</sub> (methanogenesis) by 82.72% and 133.8 %, respectively, compared to the control. These results suggested that the efficient conversion of intermediate products played a critical role in enhancing overall methane yield. Microbial community analysis identified acetotrophic methanogenesis as the dominant pathway, with <em>Methanosaeta</em> abundance reaching 91.66 % in NBW-amended treatments. Additionally, the co-occurrence network analysis showed that the air-NBW system promoted microbial interaction and stabilized the ecological network. Crucially, the NBW treatment exhibited the lowest expression of energy-dependent antioxidant enzyme genes (e.g., superoxide reductase, <em>SOR</em>), suggesting reduced oxidative stress and enhanced energy allocation toward microbial growth and methanogenic activity. By enhancing oxygen dispersion and microbial intracellular redox balance, NBW technology provides a promising approach for improving methane recovery in sustainable waste-to-energy systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132955"},"PeriodicalIF":9.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0