Bioresource Technology最新文献

{"title":"Carbonization and nitrogen plasma doping of zeolitic imidazolate framework‑8/bacterial cellulose aerogel membrane anodes for improved bioelectrocatalysis.","authors":"Bo Yan, Jiaqi Wang, Junjie Huang, Kai Chen, Yuxiang Zhu, Yun Yang, Yingzhu Guo, Xiaoxiao Guo, Dai Wang, Yufeng Zhang, Zhaohui Meng, Weiwei Cai, Rui Mu, Xue-Ao Zhang","doi":"10.1016/j.biortech.2026.136079","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136079","url":null,"abstract":"<p><p>To address the performance bottlenecks in microbial fuel cells (MFCs), we fabricate a novel N-doped zeolitic imidazolate framework-8/bacterial cellulose aerogel membrane (N/ZIF-8/BAM), integrating two-level porous with plasma-assisted high-level nitrogen doping. Carbonized bacterial cellulose provides a three-dimensional interconnected macroporous network that facilitates Bacillus subtilis colonization and transport, while ZIF-8 derived carbon introduces nanopores to expand the electrochemical active surface area. After N₂ plasma doping, N/ZIF‑8/BAM contains substantial pyrrolic N, pyridinic N, and graphitic N species. Zeta potential measurements reveal that N/ZIF‑8/BAM exhibits an overall negative surface potential (-34.6  mV), yet local positively charged N-doping sites improve bacterial adhesion at the anode interface, consistent with bacterial colonization observations. Optimized N configurations lower charge transfer resistance and promoting bio-electrocatalysis. The hierarchical porous structure enhances extracellular electron transfer between bacteria and the anode, thereby yielding a peak power density of 0.56 mW cm^-2. This work offers a feasible strategy to design high-performance anodes for MFCs.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136079"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate-bentonite immobilization with zero-valent iron nanoparticles enhances dark fermentative hydrogen production.","authors":"Chiao-Ching Chang, Yoong Kit Leong","doi":"10.1016/j.biortech.2026.136098","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136098","url":null,"abstract":"<p><p>Dark fermentation is a light-independent route for biohydrogen production, but conventional alginate immobilization may constrain mass transfer. Bentonite is an abundant, low-cost porous clay with potential to improve fermentation performance. However, its application in immobilized dark fermentation remains insufficiently investigated. This study systematically evaluated alginate-bentonite composition for immobilizing Clostridium butyricum CGS5, examined the further incorporation of zero-valent iron nanoparticles (Fe⁰NPs), and assessed the selected formulation under semi-continuous operation. Beads containing 1.125 % (w/v) sodium alginate and 1.05 % (w/v) bentonite achieved hydrogen accumulation of 409.27 ± 5.61 mL and a hydrogen yield of 1.79 ± 0.01 mol H₂/mol glucose, representing increases of approximately 70 % and 44 %, respectively, relative to suspended cells. Most bentonite-containing formulations exhibited greater resistance to compressive deformation than pure alginate beads, alongside changes in morphology and swelling behavior. Incorporating 50 mg/L Fe⁰NPs into the selected formulation further increased hydrogen accumulation to 459.17 ± 2.40 mL, an 8.1 % improvement over the Fe⁰NP-free immobilized control, without significant improvements in hydrogen production rate or yield. Following an initial 24-h batch phase, semi-continuous operation with 50 % medium replacement every 16 h prolonged hydrogen production across four feeding cycles, yielding 667.91 mL over 88 h. However, cycle-specific output varied and remained below the initial batch total. These findings support alginate-bentonite immobilization for enhanced batch biohydrogen production and identify an additional benefit of Fe⁰NP incorporation for hydrogen accumulation, while longer-term operational performance remains to be established.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136098"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient whole-cell bioconversion of indole-3-lactate by metabolic engineering of high-glucose-adaptive Lactiplantibacillus plantarum.","authors":"Cong-Rong Shi, Xiao-Juan Zhang, Zhen-Ming Lu, Yan Geng, Li-Juan Chai, Hong-Yu Xu, Jin-Song Shi, Zheng-Hong Xu","doi":"10.1016/j.biortech.2026.136089","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136089","url":null,"abstract":"<p><p>Bio-production of the high-value health-promoting metabiotic indole-3-lactate (3-ILA) by lactic acid bacteria (LAB) and bifidobacteria remains constrained to micromolar levels. This limitation is largely due to pyruvate outcompeting indole-3-pyruvate (3-IPYA) for LDH and NADH, leading to lactate accumulation and suppressed 3-ILA synthesis. In this study, previously developed high-glucose-adaptive Lactiplantibacillus plantarum strains LP-D-4G and LP-D-10E were evaluated as chassis for enhanced 3-ILA production. Compared with the parental strain, the adaptive strains exhibited increased l-tryptophan (l-Trp) utilization and 3-ILA production during resting-cell bioconversion, together with altered pyruvate-derived product distributions and intracellular NAD(H) profiles. Systematic genome mining and heterologous expression in Escherichia coli identified five active lactate dehydrogenases (LDH2/3/5/6/7) and one aromatic-amino-acid aminotransferase (ArAT2). Co-expression of LDH7 and ArAT2 in strain LP-D-4G achieved 6.06 mM 3-ILA, 0.95 mol/mol l-Trp conversion, and a 3-ILA/(3-ILA + lactate) ratio of 0.31 mol/mol in whole-cell bioconversion. Furthermore, optimization of l-Trp and glucose feeding strategies improved 3-ILA production. In a scaled-up 150-mL fed-batch system, strain LP-D-4G overexpressing LDH7 and ArAT2 produced 20.09 mM 3-ILA at 96 h, representing the highest titer reported to date for LAB-based 3-ILA production. At peak, 3-ILA yield, productivity, and the 3-ILA/(3-ILA + lactate) ratio reached 0.84 mol/mol l-Trp, 69.45 mg/L/h, and 0.25 mol/mol, respectively. This study establishes a food-grade platform for bioactive 3-ILA production and demonstrates an effective strategy to reduce lactate and enhance 3-ILA by combining a high-glucose-adaptive chassis with pathway reinforcement.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136089"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering lignin metabolism in Myceliophthora thermophila toward integrated lignocellulose valorization.","authors":"Defei Liu, Rongmei Yan, Yutong Ge, Yuying Zhang, Jingen Li, Chaoguang Tian","doi":"10.1016/j.biortech.2026.136080","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136080","url":null,"abstract":"<p><p>Lignin accounts for up to one-third of plant biomass, making it the second most abundant biopolymer on Earth. The thermophilic ascomycete Myceliophthora thermophila is a well-established platform to produce chemicals from plant biomass. However, its capacity for lignin metabolism remains largely unexplored. This study demonstrates that M. thermophila can remodel lignin and metabolize a broad spectrum of lignin-derived aromatic monomers. By integrating transcriptomic profiling with targeted gene deletions, we mapped the key metabolic pathways for these aromatic compounds. Specifically, we found that M. thermophila funnels diverse p-hydroxyphenyl (H) and guaiacyl (G) aromatic monomers into the 3,4-dihydroxybenzoic acid (protocatechuic acid, 3,4-DHB) branch of the β-ketoadipate pathway. Disrupting the protocatechuate hydroxylase and 3,4-dioxygenase genes successfully redirected metabolic flux, achieving 42.5‒95.8% molar yields of 3,4-DHB from H- and G-type monomers. Notably, the engineered strains achieved direct 3,4-DHB production from raw corncob. These findings provide new insights into the metabolism of lignin-derived aromatic compounds in M. thermophila and establish a foundation for integrated lignocellulose biorefineries and aromatic bioprocessing.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136080"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated genetic and process engineering of Pichia pastoris for high-yield ergothioneine production via methanol-free cultivation.","authors":"Jia Chen, Xin Yang, Chang-Hong Liu","doi":"10.1016/j.biortech.2026.136092","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136092","url":null,"abstract":"<p><p>Ergothioneine (EGT) is a histidine-derived thiol antioxidant with broad applications in food, pharmaceuticals and cosmetics, but its industrial production is limited by scarce natural sources and low heterologous host titers. Here, a high-yield, methanol-free EGT production platform was established in Pichia pastoris X33 via integrated genetic and process engineering: de novo pathway assembly first generated strain P4 with a shake-flask EGT titer of 99.6 ± 3.2 mg/L via screening of the strong constitutive promoter P_GCW14, site-directed engineering of Schizophyllum commune methyltransferase ScEgt1a yielded the V17A mutant, which improved the titer to 188.0 ± 8.6 mg/L in strain P11 alongside accumulated hercynine intermediate, introduction of egtB from Methylobacterium pseudosasicola (encoding the hercynine-to-cys-hercynine conversion enzyme) to drive efficient conversion of the accumulated intermediate produced strain P12 with a titer of 328.2 ± 8.3 mg/L, and further systematic optimization of culture medium and process parameters elevated the final shake-flask EGT titer to 1023.3 ± 17.9 mg/L. This work delivers the first constitutive, methanol-free production strategy for EGT, providing a scalable chassis and transferable integrated genetic-process engineering framework that reaches an advanced level among reported yeast-based EGT production systems, offering a versatile blueprint for sustainable biomanufacture of EGT and other high-value sulfur-containing natural products.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136092"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and microbial metabolic insights into o-xylene biotransformation mediated by catechol-type intermediate pathways in porous-packed biofilter.","authors":"Pengyu Li, Lin Li, Wenwen Wang, Yunping Han, Tianlong Zheng","doi":"10.1016/j.biortech.2026.136100","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136100","url":null,"abstract":"<p><p>In this study, gaseous o-xylene was removed using a laboratory-scale biotrickling filter packed with polyurethane foam cubes. The maximum elimination capacity of 35.36 g/(m³·h) can be achieved at an inlet concentration of 0.89 g/m³, a flow rate of 0.25 m³/h, and an empty bed residence time of 76 s. The adsorption of o-xylene by the packing medium followed the Freundlich isotherm model, and approximately 64 % of the o-xylene carbon was mineralized to CO₂ during steady-state operation, with the remainder utilized by microorganisms. Tax4Fun-based metabolic reconstruction revealed a 22.3 % enrichment of xenobiotic biodegradation pathways in the biofilm community relative to the planktonic phase, with the xylene degradation pathway (ko00622) enriched 1.33-fold. The o-xylene catabolic pathway was reconstructed: upper-pathway methyl activation by toluene/o-xylene monooxygenase, nearly exclusive to the biofilm; ring cleavage through hierarchical dioxygenases spanning meta-, ortho-, and gentisate routes; and lower-pathway integration into the tricarboxylic Acid cycle (TCA). Pseudomonas, Mycobacterium, and Comamonas were identified as potential functional genera. The near-absence of anaerobic benzylsuccinate pathway enzymes exclusively aerobic degradation. Metabolism-related functions dominated the biofilm functional profile (75.02 %). o-Xylene metabolism occurred predominantly on the packing-media biofilm rather than in the circulating solution, which indicated that enriching a metabolically specialized biofilm on high-surface-area porous packing is the key to efficient and stable performance. This study provides a scientific foundation for developing volatile organic compounds prevention and control strategies.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136100"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon-loss-guided metabolic engineering improved carbon atom economy and industrial feedstock utilization for l-threonine fermentation.","authors":"Jianhang Liu, Qi Sheng, Chao Ye, Xingzheng Dong, Gang Meng, Chunguang Zhao, Aiying Wei, Fei Yang, Jia Liu, Liming Liu","doi":"10.1016/j.biortech.2026.136083","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136083","url":null,"abstract":"<p><p>l-Threonine is a high-value amino acid widely used in the feed, food, and pharmaceutical industries, but its industrial production is limited by low yield. In this study, whole-genome sequencing of industrial strain Thr1 was performed, and the genome-scale metabolic model GSMM-iJHL1061 was reconstructed. The model identified 54 CO₂-related reactions and predicted 0.89 mol CO₂ release per mol l-threonine synthesized, mainly from the PPP and TCA cycle. Attenuation of decarboxylation reactions involving ilvBN, lysA, gnd, and icd, combined with RuBisCO-Prk and cynT-pyc-based CO₂ fixation, increased the yield to 0.66 g/g. Deletion of 166 non-essential genes generated Thr9, increasing the yield to 0.67 g/g. Further introduction of maltose hydrolysis and sucrose utilization systems generated Thr10, which produced 132.5 g/L l-threonine in a 1000-L fermenter, with a yield of 0.68 g/g. Overall, l-threonine yield was substantially improved through systems metabolic engineering, providing a useful reference for constructing high-yield microbial cell factories.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136083"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic engineering of COPII machinery and cell wall remodeling enables gram-level secretion of recombinant human serum albumin with native-like structural features in Kluyveromyces marxianus.","authors":"Xinyi Lu, Lunqiang Zhao, Hong Chen, Xinwei Wu, Wei Zhao, Jungang Zhou, Yao Yu, Hong Lu","doi":"10.1016/j.biortech.2026.136085","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136085","url":null,"abstract":"<p><p>Recombinant human serum albumin (rHSA) is an important alternative to plasma-derived HSA, but its microbial production is constrained by the secretion burden associated with its large size and 17 intramolecular disulfide bonds. Here, we developed a systematic secretory-network engineering strategy in the food-safe yeast Kluyveromyces marxianus. The α-factor leader enabled efficient secretion and correct N-terminal processing of rHSA. A system-wide screen of 35 SEC genes implicated coat protein complex II (COPII)-mediated endoplasmic reticulum export as a major intracellular constraint. Combinatorial engineering identified SEC23/SEC13/SEC31 as the optimal COPII module, increasing rHSA secretion by 60.7% although with a reduction in growth. Screening of 13 cell wall-related genes revealed the cell wall as an engineerable interface. Combining COPII and cell wall engineering increased extracellular rHSA by 287.3% (COPII+gas1Δkre1Δ) and 316.0% (COPII+kre6Δyps1Δ) relative to TOP1-rHSA. RT-qPCR revealed strain-specific transcriptional changes in selected COPII-, ER folding-, and cell wall-related genes, while stress phenotyping, transmission electron microscopy, and cell wall staining supported strain-specific cell wall remodeling rather than overt cell wall damage. In 5 L fed-batch fermentation with defined nutrient supplementation, the engineered strains achieved high-cell-density growth and extracellular rHSA accumulation of 2.32-2.37 g/L. Far-UV circular dichroism, Native PAGE, and LC-HRMS/MS demonstrated native-like secondary structure, non-denaturing electrophoretic mobility comparable to serum-derived HSA, and all 17 expected intramolecular disulfide linkages consistent with native HSA. This work establishes a food-safe yeast chassis through multi-layer secretion engineering for the production of rHSA with native-like structural features and provides a generalizable strategy for secreting complex disulfide-rich proteins.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136085"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-centric metagenomic analysis revealed the microbial superiority of the developed anaerobic digestion startup strategy based on combination of raw and concentrated digestate.","authors":"Jisen Xiong, Miao Yan, Wenxia Tan, Yongming Sun, Li Chen, Hailin Tian, Qian Tan","doi":"10.1016/j.biortech.2026.136090","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136090","url":null,"abstract":"<p><p>Concentrating the digestate as inoculum to initiate new anaerobic digestion (AD) reactors has been proven to be feasible. However, functional microorganisms after centrifugal concentration have poor activity in the initial stage, resulting in a long startup period. In this study, AD was initiated through addition of centrifugal concentrated digestate combined with different proportions of raw digestate (0%, 9%, 18%, and 27%, in terms of total working volume), aiming to restore the metabolic activity rapidly with a minimum amount of raw digestate to reduce the startup cost. The result showed that Cen-18% (bioaugmentation of 18% raw digestate on the top of concentrated digestate) was the most suitable startup strategy in terms of methane production rate and methane yield. Metagenomic absolute quantitative analysis revealed that methane metabolism (ko00680) was enhanced in Cen-18% (2.9 × 10¹⁰ copies/g V.S. (1.5-1.8) × 10¹⁰ copies/g in other groups) along with the AD progress by upregulating the abundance of key functional genes in carbohydrate metabolism (gapA, fbaA and eno) and amino acid metabolism (carB, argG and argH). Moreover, genome-centric metagenomic analysis implied that the Cen-18% accelerated carbon conversion by enriching higher abundance of the identified key carbohydrate and protein degraders (i.e., Bacteroidales_MAG2 and Bacteroidales_MAG8) in Cen-18% than other experimental groups. Hydrogenotrophic methanogenesis performed by Methanomicrobiales_MAG1 and Methanomicrobiales_MAG2 was the dominant methanogenic pathway in Cen-18%, which was highly symbiotic with the acetate oxidation bacteria DUMP01_MAG3, DUMP01_MAG1, and Thermosediminibacterales_MAG2. This study provides practical support for the startup of newly built biogas digesters.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136090"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Vibrio natriegens for biopesticide production: from pathway optimization to plant protection.","authors":"Xinlu Cai, Qingyue Cheng, Youquan Zou, Anni Wang, Yujie Li, Ranyang Qiu, Wenjun Zhou, Endong Yang, Ting Xue, Jing Liu","doi":"10.1016/j.biortech.2026.136078","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136078","url":null,"abstract":"<p><p>Phytopathogens pose a persistent threat to global crop production, necessitating the development of sustainable biocontrol strategies. Microbial biopesticides offer a promising alternative to chemical pesticides; however, their industrial application is frequently constrained by low production titers and high substrate costs. This study reprograms the phenazine-1-carboxylic acid (PCA) biosynthetic pathway in Vibrio natriegens, a fast-growing marine bacterium with broad carbon utilization capability. A modular plasmid toolbox is constructed through systematic optimization of promoters, ribosome binding sites, replication origins, and antibiotic resistance markers, enabling high-level PCA production. To address plasmid instability and antibiotic dependence, an I-SceI-based genome editing platform is established, facilitating scarless, multi-copy chromosomal integration of the PCA gene cluster. The final engineered strain, Vna6, achieves PCA titers of 102.0 mg/L in shake flasks and 579.8 mg/L in fed-batch fermentation with glucose as the carbon source. Furthermore, Vna6 exhibits broad-spectrum antagonistic activity against both bacterial (Streptomyces scabies) and fungal (Glomerella cingulata) phytopathogens, as confirmed by in vitro inhibition zone assays and in vivo plant protection experiments on Arabidopsis thaliana and radish seedlings. This work establishes V. natriegens as a potenial chassis for biopesticide production and offers a promising strategy for sustainable crop disease management.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136078"},"PeriodicalIF":8.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}