Bioresource Technology最新文献

{"title":"Biofilm engineering to improve succinic acid production in Escherichia coli by enhancing extracellular polysaccharide synthesis","authors":"Runze Pan ,&nbsp;Yicheng Yuan ,&nbsp;Anming Xu ,&nbsp;Wankui Jiang ,&nbsp;Wenming Zhang ,&nbsp;Jorge Barriuso ,&nbsp;Yujia Jiang ,&nbsp;Fengxue Xin ,&nbsp;Min Jiang","doi":"10.1016/j.biortech.2025.132627","DOIUrl":"10.1016/j.biortech.2025.132627","url":null,"abstract":"<div><div>Biofilms play crucial roles in enhancing microbial tolerance to environmental stress. Biofilm engineering in industrial microorganisms has been a promising and efficient approach to improve the production of metabolites. In this study, the <em>psl</em> gene cluster from <em>Pseudomonas aeruginosa</em>, for extracellular polysaccharide synthesis, was first introduced in a succinic acid (SA) producing <em>Escherichia coli</em> strain to enhance the biofilm formation ability. The engineered strain Suc260 (<em>pslA</em>-<em>J</em>) showed the improved tolerance to harsh environments and improved SA synthesis capability. Compared to the control, strain Suc260 (<em>pslA</em>-<em>J</em>) produced 70.54 g/L of SA from glucose in a 5 L bioreactor, representing an increase of 13.41 %. To further enhance the synthesis efficiency of SA, a cell immobilization fermentation system based on biofilms on alginate beads was designed. Finally, 62.66 g/L of SA with a yield of 0.76 g/g was produced from wheat straw hydrolysate in a 5 L bioreactor at the optimal pH of 6.8. When the pH was adjusted to a lower value (pH 6.0), the SA production and yield still reached 57.67 g/L and 0.75 g/g, respectively, representing 28.96 % and 42.15 % higher than those of the control strain. This study provides an efficient platform technology for the production of bio-based SA in large scale.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132627"},"PeriodicalIF":9.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of iron-based oxide-modified electrodes on the selectivity of carbon chain elongation metabolites in electro-fermentation system","authors":"Xiaoyan Sun ,&nbsp;Yanan Yin ,&nbsp;Hui Chen ,&nbsp;Lei Zhao ,&nbsp;Cheng Wang ,&nbsp;Jianlong Wang","doi":"10.1016/j.biortech.2025.132588","DOIUrl":"10.1016/j.biortech.2025.132588","url":null,"abstract":"<div><div>This study explored the electrode modification by iron-based materials (IBM) including Fe₂O₃, Fe₃O₄, and FeN in the aim of promoting the medium-chain fatty acids (MCFA) production by electro-fermentation (EF). Results showed that the modification of cathodes with IBM increased MCFA production by 15 %-169 %. FeN exhibited the best performance, achieved the maximum MCFA production of 4450.2 mg COD/L and triggered longer-chain MCFA (i.e., caprylate) production of 211.7 mg COD/L. Electrochemical analyses demonstrated that IBM modification promoted the electrochemical activity of electrodes by reducing the charge transfer resistance by 15–62 %. Microbial analysis illustrated that IBM modification promoted microbial cooperation in the system by enriching chain elongation (CE) functional microorganisms (<em>Fermentimonas</em> sp., <em>Blautia</em> sp. and <em>Anaerosalibacter</em> sp.) and electrochemically active bacteria (<em>Bacillus</em> sp.) to facilitate MCFA generation. Metabolic pathways analysis indicated that IBM modification significantly promoted the production of Acetyl-CoA for the CE process and enhanced the relative abundances of functional genes involved in reverse β-oxidation (RBO) pathway.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132588"},"PeriodicalIF":9.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing CO2 for sustainable bioelectricity: An engineered two-stage microbial co-culture approach with enhanced acetate metabolism","authors":"Xiaoman Xie,&nbsp;Ying Wu,&nbsp;Yiran Lv,&nbsp;Shuhan Dai,&nbsp;Huanhuan Li,&nbsp;Li Xu,&nbsp;Min Yang,&nbsp;Jinyong Yan,&nbsp;Yunjun Yan","doi":"10.1016/j.biortech.2025.132615","DOIUrl":"10.1016/j.biortech.2025.132615","url":null,"abstract":"<div><div>Climate change driven by rising atmospheric CO₂ levels underscores the urgent need for sustainable energy solutions. This study investigates the dual potential of CO₂ as a primary carbon source and acetate as an intermediate to simultaneously mitigate atmospheric CO₂ levels and generate bioelectricity using microbial fuel cells (MFCs). A synthetic microbial co-culture was developed, combining <em>Clostridium ljungdahlii</em> for CO₂ sequestration and <em>Shewanella oneidensis</em> MR-1 for bioelectricity production. To optimize MFC performance, <em>S. oneidensis</em> was modularly engineered to enhance acetate metabolism and electron transfer efficiency. Key modifications included upregulating ATP synthesis, introducing an ATP-independent acetate metabolic pathway, increasing NADH availability, and optimizing pili-based artificial conductive nanowires. These advancements achieved a maximum cell density (OD₆₀₀ = 0.611), a record output voltage of 351.3 mV, and a record power density of 94.9 mW/m² using acetate as the substrate. Furthermore, a two-stage biocatalytic system utilizing CO₂ as the primary carbon source yielded an output voltage of 209.3 mV and a power density of 65.0 mW/m². These results highlight the potential of engineered microbial co-culture for efficient CO₂-based bioelectricity generation, offering a scalable and sustainable pathway toward carbon–neutral energy production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132615"},"PeriodicalIF":9.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable carbon management in aerobic granular sludge for municipal wastewater treatment","authors":"Therese Areskoug ,&nbsp;Johanna Arita Mendoza ,&nbsp;Oskar Modin ,&nbsp;Dag Lorick ,&nbsp;Susanne Tumlin ,&nbsp;Britt-Marie Wilén","doi":"10.1016/j.biortech.2025.132624","DOIUrl":"10.1016/j.biortech.2025.132624","url":null,"abstract":"<div><div>Aerobic granular sludge (AGS) operated with pre-settled wastewater enables separation of organics with high biomethane potential. However, organic compounds are also needed to support denitrification, and external carbon may be needed to achieve low effluent nitrogen concentrations. This study evaluated the impact of primary sedimentation on carbon management in AGS processes. A pilot-scale reactor reached effluent nitrate concentrations of 2–3 mg NO₃-N/L when fed with pre-settled wastewater with the addition of 0.8 ± 0.2 g COD/g N as methanol in the post-denitrification phase, or when fed with raw wastewater without an external carbon source. The biogas potential of the whole process was 25 % higher with primary sedimentation. A sustainability assessment showed that the benefits of increased biogas production with primary sedimentation could outweigh the drawbacks associated with the use of methanol as external carbon source both in terms of economy and CO₂ emissions, but methane price and biogas yield affect the assessment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132624"},"PeriodicalIF":9.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere as hotspot for ammonia oxidation in secondary effluent constructed wetlands: Role of comammox Nitrospira","authors":"Yize Zheng,&nbsp;Bowen Wang,&nbsp;Hongwei Chen,&nbsp;Jinyang Zhou,&nbsp;Chao Song,&nbsp;Jin Chen,&nbsp;Zimin Chai,&nbsp;Maosheng Zheng","doi":"10.1016/j.biortech.2025.132621","DOIUrl":"10.1016/j.biortech.2025.132621","url":null,"abstract":"<div><div>Secondary effluent constructed wetlands (SECWs) are engineered ecosystems for advanced wastewater treatment, yet the functional roles and survival strategies of complete ammonia oxidizers (comammox) within these systems remain poorly understood, particularly from a rhizosphere view. The results of this study demonstrated that comammox was numerically and functionally dominant (60.4 % to 70.6 %) in SECWs. The rhizosphere acted as a hotspot for ammonia oxidation and N₂O production, compared to the nonrhizosphere. Enhanced nitrification was attributed to radial oxygen loss and humic acid-like compounds in root exudates. Furthermore, variations in comammox community structure and ammonia kinetic properties (K_m(app) = 0.140 ± 0.026 mg N L⁻¹) revealed niche differentiation among comammox species: the r-strategist <em>Nitrospira</em> sp. HN-bin3 thrived over time, whereas the K-strategist <em>Nitrospira nitrificans</em> was outcompeted, with ammonia concentration identified as the main driving factor. These results highlighted the vital but underappreciated role of comammox in the nitrogen cycle of constructed wetlands and provided new insights into their ecological functions and adaptive strategies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132621"},"PeriodicalIF":9.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into hydrochar’s physicochemical properties evolution and nitrogen migration mechanism during co-hydrothermal carbonization of microalgae and corn stalk with FeCl3/NH4Cl/melamine addition","authors":"Yang Peng ,&nbsp;Xianqing Zhu ,&nbsp;Qian Shen ,&nbsp;Yun Huang ,&nbsp;Ao Xia ,&nbsp;Jingmiao Zhang ,&nbsp;Xun Zhu ,&nbsp;Qiang Liao","doi":"10.1016/j.biortech.2025.132612","DOIUrl":"10.1016/j.biortech.2025.132612","url":null,"abstract":"<div><div>Co-hydrothermal carbonization (co-HTC) of microalgae and lignocellulosic biomass can boost co-hydrochar yield and nitrogen retention rate, but the co-hydrochar still suffers from low nitrogen content and porosity. Accordingly, in this study, FeCl₃, NH₄Cl and melamine were employed as additives to intensify the co-HTC process of microalgae (CP) and corn stalk (CS), and their impacts on the co-hydrochar yield, physicochemical properties and nitrogen transformation pathways were firstly investigated comprehensively. The results indicated that adding FeCl₃ increased co-hydrochar’s specific surface area, but the oxidation power of Fe³⁺ facilitated nitrogen into the aqueous-phase products, leading to the nitrogen distribution in aqueous-phase products reaching up to 83.51 %. Melamine incorporation increased the nitrogen content of co-hydrochar (6.95 %) and oil-phase products, while reduced co-hydrochar’s porosity. NH₄Cl was the most effective additive for nitrogen-doped hydrochar production, simultaneously increasing the yield (40.24 %), nitrogen content (8.93 %) and specific surface area (9.12 m² g⁻¹) of co-hydrochar. The nitrogen transformation mechanism during NH₄Cl-assisted co-HTC process could be divided into two stages. At the first stage (200 ℃–240 ℃), the NH₄⁺ in NH₄Cl could react with hydrolysis intermediates (Maillard and Mannich reactions) to generate heterocyclic nitrogen compounds entering the oil-phase, which would further react with the oxygen-containing functional groups to facilitate the nitrogen enrichment in the co-hydrochar. At the second stage (240 ℃–280 ℃), a portion of hydrochar would undergo secondary degradation and formed water-soluble nitrogen-rich small molecules, which were transferred to the aqueous-phase products again. This study demonstrated the high feasibility of additive-assisted co-HTC for producing high-quality nitrogen-rich carbon materials.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132612"},"PeriodicalIF":9.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maturity phase is crucial for removing antibiotic resistance genes during composting: novel insights into dissolved organic matter-microbial symbiosis system","authors":"Bing Bai ,&nbsp;Lixia Wang ,&nbsp;Fachun Guan ,&nbsp;Houan Pi ,&nbsp;Anxun Wang ,&nbsp;Limei Zhai","doi":"10.1016/j.biortech.2025.132607","DOIUrl":"10.1016/j.biortech.2025.132607","url":null,"abstract":"<div><div>Composting is widely regarded as an effective method for reducing antibiotic resistance genes (ARGs) in livestock and poultry manure. However, the critical mechanisms of ARGs in different composting phase are still unclear. In this study, normal composting and two types of rapid composting (without mature phase) were used to analyze the removal of ARGs and the succession of dissolved organic matter (DOM). Compared to normal composting, rapid composting reactivated <em>tetracyclines</em>, <em>sulfonamide</em>, and <em>quinolones</em> resistance genes during the maturation phase and reduced the total ARGs removal rates by 45.58 %–57.87 %. Humus-like components could inhibit the proliferation of ARGs, and the enrichment of protein-like components increased abundances of <em>Pusillimonas</em>, <em>Persicitalea</em>, and <em>Pseudomonas</em>, indirectly reducing the removal. This study is the first to demonstrate the contribution of DOM and microbial community to ARGs removal, emphasizing the importance of the maturation phase for ARGs elimination. This research provides guidance for producing safe compost products.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132607"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applicability of temperature-phased anaerobic digestion in enhancing methanation of high-solid sludge: Process performance, microbial community analysis and energy balance assessment","authors":"Li-Jie Wu,&nbsp;Fei Ye,&nbsp;Fan Yang,&nbsp;Yong-Kang Lyu","doi":"10.1016/j.biortech.2025.132614","DOIUrl":"10.1016/j.biortech.2025.132614","url":null,"abstract":"<div><div>High-solid anaerobic digestion has been paid more attention, expected to solve the increasing amount of sewage sludge. In order to cope with the new issues of high-solid sludge digestion, recently emerging thermophilic (stage I)-mesophilic (stage II) temperature-phased anaerobic digestion (TPAD) process was employed to probe into its applicability in enhancing methanation. High-solid sludge at a total solid (TS) of above 15 % was fed to a TPAD process and a single-stage mesophilic digestion (MD) process continuously. The increasing loadings from 3.96 g chemical oxygen demand (COD)/L/d to 8.05 g COD/L/d were set by gradually shortening hydraulic retention time from 20 d to 10 d. Methane yield could be increased from 0.11 L/g COD_added to 0.15 L/g COD_added, with 10 % higher TS removal achieved in the TPAD. The reason could be attributed to improved hydrolysis of the main fraction protein. Despite acetic acid accumulation in stage I, surplus alkalinity supply rendered acid/alkalinity ratios much lower. The interaction between the 2 stages offered more diverse microbial community, which led to intensive adaptive ability to external shocking. The density of archaea for stage II /stage I increased nearly linearly with higher organic loading. As high as around 60 % <em>Methanosarcina</em> became the main mesophilic archaea. The dominant functional bacteria <em>Firmicutes</em> in stage II was also promoted. On the premise of enhanced conversion efficiency, additional energy input from heat requirement of thermophilic stage in the TPAD was proven to be compensated by improved methane production, leading to similar or even higher net energy production with the MD.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132614"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional biochar and catalytic sustainable biosynthesis of PHA derived from waste activated sludge","authors":"Zhaozhou Peng ,&nbsp;Lei Zhang ,&nbsp;Panpan Li ,&nbsp;Junzhang Li ,&nbsp;Chen Shen ,&nbsp;Xia Tian ,&nbsp;Shouxin Liu","doi":"10.1016/j.biortech.2025.132613","DOIUrl":"10.1016/j.biortech.2025.132613","url":null,"abstract":"<div><div>Advancing circular wastewater management, this study developed an innovative solution for waste activated sludge (WAS) utilisation by constructing a system in which<!--> <!-->WAS was<!--> <!-->converted to biochar and feedback catalysed biosynthesis of Polyhydroxyalkanoates (PHA). After PHA extraction, residual sludge was pyrolysed into biochar, which acted as a catalyst to further enhance PHA production by the self-fermentation conversion of WAS. Biochar significantly improved electron transfer during anaerobic digestion (by 61.36 %) and boosted the proportion of PHA-producing bacteria by 12.33%, resulting in an 81.04% increase in PHA yield, comprising 66.14% of the dry sludge weight. Multi-omics analysis showed that biochar amplified the expression of key PHA synthesis genes (phaA, 4.69-fold;<!--> <!-->phaB,<!--> <!-->10.04-fold; and<!--> <!-->phaC,<!--> <!-->12.02-fold). This study offers a sustainable approach for WAS management, thereby advancing resource efficiency and providing ecological benefits.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132613"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid formation of partial denitrification biofilm using gas–liquid separation membrane as carrier: Performance and mechanism","authors":"Yong-Tao Lv ,&nbsp;Jin Zhang ,&nbsp;Ting Sun ,&nbsp;Jian Dong ,&nbsp;Yongbao Pan ,&nbsp;Yixin Wang ,&nbsp;Xudong Wang ,&nbsp;Lei Wang","doi":"10.1016/j.biortech.2025.132611","DOIUrl":"10.1016/j.biortech.2025.132611","url":null,"abstract":"<div><div>Partial denitrification (PD) can ensure stable supply of electron acceptors for anaerobic ammonia oxidation, and biofilm is an effective method to prevent biomass loss, which are crucial for stable operation of PD. In this study, hydrophobic hollow-fiber gas–liquid separation membranes were placed in a denitrification sequencing batch reactor, and dense biofilms were formed within just 3 days. Confocal laser microscopy showed the preferential attachment of the protein (PN) content in extracellular polymeric substances (EPS) to the membrane surface, followed by exopolysaccharides. Further analyses showed the decrease in the types of signal molecules from six to two (i.e., C4-HSL, C6-HSL) due to negative pressure operation. Importantly, the concentration of C4-HSL increased dramatically with the increase in PN concentration, suggesting that negative pressure promoted the synthesis of C4-HSL signal molecules, which further mediated the secretion of PN for biofilm formation. In addition, biofilm formation was accompanied by nitrite accumulation, leading to successful achievement of PD. Furthermore, 60 % of nitrate-to-nitrite transformation ratio was obtained even when COD/N was increased from 4.5 to 5.0 and influent nitrate concentration was reduced to 25 mg/L. This confirmed the stability of PD, which was mainly attributed to a change in the microbial community and a decrease in nitrite reductase (Nir) activity, with microorganisms enriched through the gas–liquid separation operation exhibiting low Nir activity. This study provides a new method for rapid formation of biofilm for wastewater treatment and stable operation of PD.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"431 ","pages":"Article 132611"},"PeriodicalIF":9.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}