Bioresource Technology最新文献_第4页

Activated carbon facilitates Saccharomyces cerevisiae colonization in an electron-donor self-sustaining chain elongation fermentation system. 活性炭促进了电子供体自持链延伸发酵系统中酿酒酵母的定植。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133116

Weizhong Huo, Zhaofan Lin, Rong Ye, Jing Yu, Ruifu Zhang, Qirong Shen

{"title":"Activated carbon facilitates Saccharomyces cerevisiae colonization in an electron-donor self-sustaining chain elongation fermentation system.","authors":"Weizhong Huo, Zhaofan Lin, Rong Ye, Jing Yu, Ruifu Zhang, Qirong Shen","doi":"10.1016/j.biortech.2025.133116","DOIUrl":"10.1016/j.biortech.2025.133116","url":null,"abstract":"Chain elongation is a promising technology for the resourceful utilization of organic waste and wastewater. Glucose and starch were used as model substrates to demonstrate that exogenous electron donors are essential for chain elongation. Glucose and starch affects caproate synthesis in the chain elongation lacking of electron donor producing microorganisms, while glucose and ethanol significantly influence the succession of microbial community. Activated carbon enhanced the colonization of Saccharomyces cerevisiae and strengthened the chain elongation process, with the relative abundance of S. cerevisiae significantly increasing by 28.3 % and 176.1 % in the presence of 5 g/L and 10 g/L of activated carbon, respectively. The reverse β-oxidation (RBO) cycle was identified as the primary metabolic pathway for caproate production. The relative abundance of genes encoding key enzymes in the RBO pathway showed increase. This study provides valuable insights into optimizing chain elongation, paving the way for sustainable and efficient organic waste valorization technologies.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133116"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding critical CAZyme genes and transcription factors for pathogen-suppressing lignocellulosic biomass valorization via fermentation. 木质纤维素生物质通过发酵抑制病原体的生物增值：解码关键的碳水化合物活性酶基因和相关转录因子。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133102

Peng Ren, Tianjie Yang, Xinlan Mei, Xiaofang Wang, Yangchun Xu, Qirong Shen, Zhong Wei

{"title":"Decoding critical CAZyme genes and transcription factors for pathogen-suppressing lignocellulosic biomass valorization via fermentation.","authors":"Peng Ren, Tianjie Yang, Xinlan Mei, Xiaofang Wang, Yangchun Xu, Qirong Shen, Zhong Wei","doi":"10.1016/j.biortech.2025.133102","DOIUrl":"10.1016/j.biortech.2025.133102","url":null,"abstract":"Carbohydrate-active enzyme (CAZyme) genes and their transcription factors (TFs) are crucial for the fermentation of lignocellulosic biomass to inhibit pathogen. However, the diversity of CAZyme genes and the complexity of TFs identification limit the efficient biovalorization of bio-resources. This study aimed to inhibit the pathogen Ralstonia solanacearum by fermenting two substrates (chrysanthemum and peanut stems) with Bacillus amyloliquefaciens, while employing multi-omics and machine learning to analyze key CAZyme genes as well as their TFs. The results showed that the water-soluble extracts (WSEs) from fermented chrysanthemum stem (days 6-7) exhibited strong antimicrobial activity. Glycosyl transferase (GT) and polysaccharide lyase (PL) gene sets were enriched significantly during chrysanthemum stem fermentation. Genes, encoding a UDP-glycosyltransferase (GT1) and a pectin lyase (PL1), were identified as key and correlated with the inhibition rate significantly. Through computational prediction, we further revealed the sigma factor RpoE as an indirect positive regulator of PL1 gene expression. Our study provides valuable insights into the identification of key CAZyme genes and the rapid discovery of their TFs, offering a foundation and promising direction for future optimization of fermentation technology.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133102"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulase-friendly dual-functional deep eutectic solvents for enhancing reactivity of dissolving pulp to produce cellulose acetate. 纤维素酶友好型双功能深共晶溶剂用于提高溶解纸浆的反应性以生产醋酸纤维素。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-20 DOI: 10.1016/j.biortech.2025.133189

Hui Zhao, Zijun Gao, Meixin Wang, Xin Li, Wenqiu Zheng, Deqiang Li, Feng Xu

{"title":"Cellulase-friendly dual-functional deep eutectic solvents for enhancing reactivity of dissolving pulp to produce cellulose acetate.","authors":"Hui Zhao, Zijun Gao, Meixin Wang, Xin Li, Wenqiu Zheng, Deqiang Li, Feng Xu","doi":"10.1016/j.biortech.2025.133189","DOIUrl":"10.1016/j.biortech.2025.133189","url":null,"abstract":"High-quality dissolving pulp is essential for cellulose acetate (CA) production. However, the inherent stability of existing dissolving pulp significantly impedes chemical penetration, posing a huge challenge. Here, a novel deep eutectic solvent (DES)-assisted endoglucanase-enriched cellulase (EG) process is developed to enhance the pulp reactivity, facilitating the production of acetate-grade dissolving pulp. DES fulfills the dual functionality, specifically disrupting hydrogen bonds of cellulose to promote fiber swelling and acting as a main component within the buffer to protect the cellulase activity. Zinc acetate-based DES-assisted EG (ZAC-EG) treatment profoundly alters the structural properties of the pulp by reducing the degree of polymerization from 1227 to 958, decreasing the crystallinity and augmenting the crystallite size. Furthermore, the acetylation value significantly increases from 331 to 1148, comfortably surpassing the standards set for acetate-grade dissolving pulp. CA films prepared from ZAC-EG pulp exhibit high light transmittance (98.6 %) and tensile strength (62.2 ± 3.2 MPa), surpassing commercial CA films. This work presents a green and efficient method for the industrial production and utilization of acetate-grade dissolving pulp.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"437 ","pages":"133189"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-dosage hydrophobically modified copolymers as effective promoters in enzymatic hydrolysis of lignocellulosic biomass. 低剂量疏水改性共聚物作为木质纤维素生物质酶解的有效促进剂。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133103

Li Xu, Hongming Lou, Yuxia Pang, Xueqing Qiu

{"title":"Low-dosage hydrophobically modified copolymers as effective promoters in enzymatic hydrolysis of lignocellulosic biomass.","authors":"Li Xu, Hongming Lou, Yuxia Pang, Xueqing Qiu","doi":"10.1016/j.biortech.2025.133103","DOIUrl":"10.1016/j.biortech.2025.133103","url":null,"abstract":"Amphiphilic surfactants have shown promise as enhancers for lignocellulosic enzymatic saccharification, but conventional additives often perform poorly at low dosage. To overcome this limitation, we engineered poly(AM-b-Sty) via micellar polymerization, incorporating hydrophobic styrene (Sty) units into a hydrophilic acrylamide (AM) backbone. This design increases the additive's affinity for lignin surfaces while minimizing nonproductive cellulase adsorption onto residual lignin. The optimized copolymer (15.4 mol% Sty) outperformed conventional additives (e.g., PEG, CTAB, Tween-80, BSA), boosting glucose yields from 37.6% to 67.7% at low loading (0.5 g/L, 25 mg/g glucan) and cutting enzyme dosage by 67% (to 5 FPU/g glucan). Mechanistic studies showed that hydrophobic chains in poly(AM-b-Sty) increased its adsorption capacity on lignin by 53-fold compared to the polymer without hydrophobic segments, while the AM chains formed a hydrated barrier on lignin surfaces. Consequently, nonproductive cellulase binding was reduced by 65%. Furthermore, hydrophobic units synergistically formed protective layers at the air-liquid interface and stabilized cellulase secondary structure, minimizing activity loss under environmental stresses. This work establishes hydrophobicity-engineered copolymers as effective lignin-blocking agents and provides mechanistic insights for rational additive design.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133103"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing bioenergy from water hyacinth: pretreatment and anaerobic co-digestion. 提高水葫芦的生物能：预处理和厌氧共消化。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133070

Adanyro Atilago, Sayon Dit Sadio Sidibé, Seyram Kossi Sossou, Maureen Norah Nabulime, Emily Lim McCoy, Marie Sawadogo, Stephanie Lansing

引用次数: 0

Predicting and interpreting nitrogen removal performance and functional microbial abundance of single-stage partial nitrification and anammox system using machine learning methods. 利用机器学习方法预测和解释单级部分硝化和厌氧氨氧化系统的脱氮性能和功能微生物丰度。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133119

Xiulin Mu, Fangxu Jia, Shengming Qiu, Yiran Li, Ning Mei, Xingcheng Zhao, Baohong Han, Xiangyu Han, Jingjing Zhang, Hong Yao

{"title":"Predicting and interpreting nitrogen removal performance and functional microbial abundance of single-stage partial nitrification and anammox system using machine learning methods.","authors":"Xiulin Mu, Fangxu Jia, Shengming Qiu, Yiran Li, Ning Mei, Xingcheng Zhao, Baohong Han, Xiangyu Han, Jingjing Zhang, Hong Yao","doi":"10.1016/j.biortech.2025.133119","DOIUrl":"10.1016/j.biortech.2025.133119","url":null,"abstract":"Machine learning (ML) was employed to simultaneously predict nitrogen removal rate (NRR) and functional microbial abundance of single-stage partial nitrification and anammox (PNA) system. Shapley additive explanations (SHAP) and causal inference were used to analyze the impact of key factors and their optimal ranges. Artificial neural network (ANN) and extreme gradient boosting (XGBoost) have strong predictive abilities for NRR (R2 = 0.94) and functional microbial abundance (R2 ≥ 0.57), respectively. pH and free ammonia (FA) are important factors affecting NRR. To inhibit nitrite oxidizing bacteria (NOB), it was recommended that FA be maintained above 5 mg/L, while O2 be kept below 0.4 mg/L. Candidatus Brocadia-dominated sludge is recommended under low nitrogen (NH4+-Ninf < 200 mg/L) or O2 fluctuation environments, while Candidatus Kuenenia-dominated sludge is recommended under high nitrogen (NH4+-Ninf > 400 mg/L), low temperature (20-30°C), or pH fluctuations (7.4-8.4). These models provide prospects and references for the application of PNA technology.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133119"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hierarchical nano-architectonics of porous carbon from an interpenetrating Gel-CNTs network via salt-templated strategy for high-performance supercapacitors. 基于盐模板策略的凝胶-碳纳米管互穿网络多孔碳的分层纳米结构用于高性能超级电容器。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133083

Xin Hou, Penggang Ren, Wenhui Tian, Tong Wu, Jiayi Wang, Xiaojie Zhen, Zhengyan Chen, Yanling Jin

{"title":"Hierarchical nano-architectonics of porous carbon from an interpenetrating Gel-CNTs network via salt-templated strategy for high-performance supercapacitors.","authors":"Xin Hou, Penggang Ren, Wenhui Tian, Tong Wu, Jiayi Wang, Xiaojie Zhen, Zhengyan Chen, Yanling Jin","doi":"10.1016/j.biortech.2025.133083","DOIUrl":"10.1016/j.biortech.2025.133083","url":null,"abstract":"Biomass-derived carbon materials offer considerable potential for sustainable supercapacitors (SCs) electrodes, yet the practical application is often limited by their unstable structure, insufficient continuous conductive networks and inevitable corrosive activation processes. Herein, hierarchical porous carbon materials were fabricated through a nano-architectonic strategy that combines salt-templated activation with the construction of conductive network. Specifically, NaNO3 serves simultaneously as a sacrificial template and a mild activator to generate interconnected hierarchical frameworks, while the interpenetrating gelatin/carbon nanotubes (CNTs) network establishes continuous 3D conductive pathways that support efficient electron transport and preserve structure integrity. The resulting carbon exhibits a high specific surface area (2204.2 m2/g), hierarchical porosity, and enhanced electrical conductivity, which endow the material with high capacitance performance and reliable electrochemical stability. In a three-electrode system, the CNGC-derived electrode delivers a specific capacitance of 351.7F/ g at 1 A/g, retaining 70.1 % at 50 A/g. The symmetric supercapacitor assembled with Na2SO4 electrolyte achieves an energy density of 40.84 Wh/kg at 1000 W/kg and maintains 98.3 % capacitance retention over 10,000 cycles. The rational combination of biomass-derived molecular networks, nanoscale conductive fillers, and salt-templated pore architecture provides a promising pathway toward structurally coherent and functionally integrated carbon materials for electrochemical energy storage.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133083"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Pichia pastoris as an industrial chassis enables biosynthesis of dopamine from methanol. 毕赤酵母作为工业底盘的代谢工程使甲醇生物合成多巴胺成为可能。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-11-01 Epub Date: 2025-06-29 DOI: 10.1016/j.biortech.2025.132915

Dexun Fan, Zijia Yuan, Huayang Tang, Pengcheng Ren, Shuangyan Han

{"title":"Metabolic engineering of Pichia pastoris as an industrial chassis enables biosynthesis of dopamine from methanol.","authors":"Dexun Fan, Zijia Yuan, Huayang Tang, Pengcheng Ren, Shuangyan Han","doi":"10.1016/j.biortech.2025.132915","DOIUrl":"10.1016/j.biortech.2025.132915","url":null,"abstract":"Methanol, as a renewable and carbon-neutral single-carbon (C1) feedstock, has emerged as an ideal carbon source for green biomanufacturing due to its non-competition with food resources and scalability for industrial production. Here, we report the first efficient biosynthesis of dopamine from methanol through systematic metabolic engineering strategies in P. pastoris. Specifically, overexpressing high-activity tyrosine hydroxylase and enhancing shikimate pathway flux yielded 579 mg/L dopamine. To prevent degradation, key dopamine catabolic enzymes (PAS_chr1-4_0441) were knocked out. By reinforcing NADH regeneration and accelerating methanol assimilation, the titer increased to 1533 mg/L, an 84.2-fold increase from the first-generation strain. Finally, we optimized the fermentation process in a 15 L fermenter to minimize dopamine autoxidation, achieving a highest reported dopamine titer using methanol as the sole carbon source to date (12.2 g/L). This study not only validates methanol as a high-performance substrate for industrial microbiology, but also establishes a critical foundation for synthesizing dopamine and its derivatives from C1 feedstocks.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132915"},"PeriodicalIF":9.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quorum sensing mediates spatiotemporal microbial community dynamics and nitrogen metabolism in biofloc-based Litopenaeus vannamei aquaculture systems. 群体感应介导生物絮团型凡纳滨对虾养殖系统微生物群落时空动态和氮代谢

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-10-07 DOI: 10.1016/j.biortech.2025.133459

Ni Liu, Yuhang Zhang, Yuanshuai Zhang, Yun Yang, Hao Long, Aiyou Huang, Yanhua Zeng, Zhenyu Xie

{"title":"Quorum sensing mediates spatiotemporal microbial community dynamics and nitrogen metabolism in biofloc-based Litopenaeus vannamei aquaculture systems.","authors":"Ni Liu, Yuhang Zhang, Yuanshuai Zhang, Yun Yang, Hao Long, Aiyou Huang, Yanhua Zeng, Zhenyu Xie","doi":"10.1016/j.biortech.2025.133459","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133459","url":null,"abstract":"Biofloc technology (BFT) enables sustainable aquaculture by leveraging microbial communities to enhance water quality and nutrient cycling. However, the role of quorum sensing (QS) in regulating microbial dynamics and metabolic functions within BFT systems remains poorly understood. This study examined how QS spatiotemporally regulates microbial succession and nutrient metabolism in a biofloc-based Litopenaeus vannamei aquaculture system over 82 days culture. Ammonia and nitrite concentrations shifted through four phases: initial (IP), rising (RP), declining (DP), and stabilization (SP). Notably, nitrite levels decreased rapidly from 1.21 mg/L to 0.03 mg/L during DP. Metagenomic analysis revealed Pseudomonadota, Actinomycetota, and Bacteroidota as the consistently dominant phyla, while dominant genera shifted over time. QS pathways displayed temporal heterogeneity: acyl-homoserine lactones (AHLs) and autoinducer-2 (AI-2) predominated during IP, whereas aromatic hydrocarbon kinases (AHKs) and cyclic dimeric guanosine monophosphate (c-di-GMP) were more enriched during SP. KEGG analysis indicated that nitrogen metabolism genes were more abundant in bioflocs than in the water. Genes associated with dissimilatory nitrate reduction and denitrification were significantly more abundant than those involved in other nitrogen metabolic processes (p < 0.05). Furthermore, QS signaling coordinated the complex interaction networks among 30 dominant bacterial genera (e.g., Amaricoccus and Ruegeria) involved in carbon, nitrogen, and sulfur metabolism, which is crucial for maintaining the stability and functionality of the biofloc system. This study elucidates the mechanisms through which microbial signaling orchestrates efficient nitrogen removal and sustains system stability, thereby providing a foundation for developing targeted bioaugmentation strategies to enhance sustainable aquaculture practices.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133459"},"PeriodicalIF":9.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signaling role of 6-benzylaminopurine in enhanced biotreatment of saline wastewater: performance and mechanisms. 6-苄基氨基嘌呤在强化含盐废水生物处理中的信号作用：性能和机制。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-10-07 DOI: 10.1016/j.biortech.2025.133455

Huiwen Yang, Bin Cui, Dandan Zhou

{"title":"Signaling role of 6-benzylaminopurine in enhanced biotreatment of saline wastewater: performance and mechanisms.","authors":"Huiwen Yang, Bin Cui, Dandan Zhou","doi":"10.1016/j.biortech.2025.133455","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.133455","url":null,"abstract":"This study introduced the phytohormone 6-benzylaminopurine (6-BA) as a novel, economical, and eco-friendly bacterial signal molecule (SM), which overcame the cost and instability limitations of acyl-homoserine lactones (AHLs) in high-salinity wastewater treatment. 6-BA bound to histidine kinases in two-component systems (TCS) through hydrogen bonding, triggering downstream signal transduction and metabolic regulation. Under high-salinity stress, 6-BA promoted cellular integrity and ionic homeostasis, increasing live-cell counts by 113.7%. To mitigate phenol toxicity, 6-BA enhanced extracellular polymeric substance (EPS) functions and antioxidant systems, reducing reactive oxygen species (ROS) by 19.8%. 6-BA upregulated genes related to DNA replication, the TCA cycle, and fatty acid synthesis, thereby repairing membrane integrity. 6-BA also enriched degrading enzymes and improved phenol degradation, leading to approximately 20% increases in COD, TN, and TP removal. Crucially, 6-BA restructured the microbial community, reducing antibiotic resistance gene (ARG) host abundance by 27.9% and ARG-encoding plasmids by 32.8, which curtailed horizontal gene transfer risks. Additionally, 6-BA exhibited no observable ecotoxicity. This work proposed 6-BA signaling as a novel bioaugmentation strategy for enhanced remediation of high-salinity wastewater.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133455"},"PeriodicalIF":9.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0