Bioresource Technology最新文献_第3页

Novel laser-driven fast pyrolysis of biomass: Insights into biochar characteristics and applications 新型激光驱动快速热解生物质：洞察生物炭的特性和应用。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132986

Rui Ma , Jintao Luo , Xiaoning Duan , Yuwan Sheng , Shaohui Wang , Deli Zhang , Fang Wang , Shuo Yang , Weiming Yi

{"title":"Novel laser-driven fast pyrolysis of biomass: Insights into biochar characteristics and applications","authors":"Rui Ma , Jintao Luo , Xiaoning Duan , Yuwan Sheng , Shaohui Wang , Deli Zhang , Fang Wang , Shuo Yang , Weiming Yi","doi":"10.1016/j.biortech.2025.132986","DOIUrl":"10.1016/j.biortech.2025.132986","url":null,"abstract":"<div><div>Biomass pyrolysis is a promising technology for producing biochar, but conventional methods often lack precise control over heating rates and real-time monitoring, particularly in fast pyrolysis. A novel laser-driven fast pyrolysis (LFP) system was developed, achieving ultrafast heating rates (100°C/s) and enabling non-contact temperature measurement through infrared thermometry. Experiments were conducted using poplar wood as feedstock, with heating rates of 10°C/s and 100°C/s, spanning temperatures from 400°C to 700°C. Results showed that fast heating significantly reduced biochar yield, predominantly below 400°C due to rapid devolatilization. The pore volume and specific surface area began increasing within a distinct temperature range (500–600°C), independent of heating rates. However, at 600°C, pore volume decreased by sixfold (0.127 → 0.021 ml/g) and specific surface area by eightfold (223.71 → 27.83 m<sup>2</sup>/g) under fast heating, accompanied by a four orders of magnitude electrical resistivity decrease (4.68e4 → 5.81 Ω·cm). These findings demonstrate the feasibility of the LFP system for providing critical technical parameters and guidance in optimizing biochar properties, particularly under fast heating conditions. By elucidating the effects of heating rates on biochar yield, porosity, specific surface area, and electrical resistivity, this study offers valuable insights into sustainable biomass conversion technologies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132986"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single and co-cultivation of Chlorella vulgaris and Pseudomonas aeruginosa: Efficient nutrient removal and lipid/starch formation by photoperiodic co-culture 普通小球藻和铜绿假单胞菌的单独和共培养：光周期共培养的有效营养去除和脂质/淀粉形成。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132983

Majed Tavakol, Fereshteh Naeimpoor

{"title":"Single and co-cultivation of Chlorella vulgaris and Pseudomonas aeruginosa: Efficient nutrient removal and lipid/starch formation by photoperiodic co-culture","authors":"Majed Tavakol, Fereshteh Naeimpoor","doi":"10.1016/j.biortech.2025.132983","DOIUrl":"10.1016/j.biortech.2025.132983","url":null,"abstract":"<div><div>Co-cultivation of <em>Chlorella vulgaris</em> and <em>Pseudomonas aeruginosa</em> was investigated, aiming at enhanced nutrient removal and storage material formation. The effect of the initial level of C-source (glucose), inoculum percentage of bacteria (0–100) and trophic conditions (hetero- and mixo-) was investigated, resulting in selection of 2 g/L glucose and 50 % bacteria in inoculum under mixotrophic condition. This led to the highest nutrient removal (43.6 % nitrate, 96.7 % glucose and 99.5 % phosphate) and improved cell contents of lipid (20.8 %) and starch (10.9 %), surpassing those of single microalgal culture. Additionally, six photoperiodic co-cultivations with a preparatory heterotrophic condition (48 h), followed by mixotrophic conditions (continuous and cyclic lighting), were applied at three initial nitrate levels (1200, 800, and 400 mg/L). Continuous lighting and nitrate at 400 mg/L led to complete nutrient removal and the highest lipid (633.8 mg/L) and starch (493.9 mg/L) production. These results validate the potential of photoperiodic co-cultures of bacteria and microalgae for wastewater treatment alongside lipid and starch production, which can serve as valuable biofuel resources.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132983"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waste biomass durian shell carbon to derive N-rich porous microbial fuel cell anode for simultaneous dye degradation and electricity generation 废生物质榴莲壳碳可衍生富n多孔微生物燃料电池阳极，用于同时降解染料和发电。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132984

Xiaoyan He , Shuo Ma , Penggai Ma , Runze Cao , Xing Tian , Yuanyuan Lu , Jing Li , Yanling Liang , Zhiqiang Wang , Xiaoquan Lu

{"title":"Waste biomass durian shell carbon to derive N-rich porous microbial fuel cell anode for simultaneous dye degradation and electricity generation","authors":"Xiaoyan He , Shuo Ma , Penggai Ma , Runze Cao , Xing Tian , Yuanyuan Lu , Jing Li , Yanling Liang , Zhiqiang Wang , Xiaoquan Lu","doi":"10.1016/j.biortech.2025.132984","DOIUrl":"10.1016/j.biortech.2025.132984","url":null,"abstract":"<div><div>Waste biomass is an ideal material for electrode modification of low-cost and high-performance microbial fuel cell (MFC). MFC is mainly committed to effectively improve the power generation performance and degradation performance through anode modification. In this study, a biomass-derived <em>N</em>-rich 3D macroporous MFC anode (PPy-DSC/CF) with high conductivity, superhydrophilicity, and biocompatibility was successfully prepared by in-situ polymerization of pyrrole on the surface of carbonized durian shell (DS) using carbon mats as the substrate material. PPy successfully increased the pyrrole-N content of the DSC, and the more graphitized structure could improve the conductivity of the biochar. High electrical conductivity of biochar catalyzes the RhB degradation by electron transfer. Meanwhile, the rich macroporous structure of 3D PPy-DSC/CF electrode material is crucial for the attachment, growth, biofilm formation and electron transfer of electroactive bacteria. It achieved the highest power density output of 5184 mW/m<sup>3</sup> and 91.26 % COD removal efficiency. The RhB degradation efficiency reached 96.36 %. This study provides a new strategy for biomass-derived porous carbon electrodes for energy utilization and MFC electrochemical systems to achieve environmental sustainability.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132984"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering rapid proliferation of filamentous bacteria in activated sludge community via cross-feeding of public goods mediated by diffusible signal factor 可扩散信号因子介导的公共物品交叉喂养对活性污泥群落丝状菌快速增殖的解读

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132980

Huan Liu , Jie Li , Shao-Yang Liu , Jin-Song Guo , You-Peng Chen , Peng Yan

{"title":"Deciphering rapid proliferation of filamentous bacteria in activated sludge community via cross-feeding of public goods mediated by diffusible signal factor","authors":"Huan Liu , Jie Li , Shao-Yang Liu , Jin-Song Guo , You-Peng Chen , Peng Yan","doi":"10.1016/j.biortech.2025.132980","DOIUrl":"10.1016/j.biortech.2025.132980","url":null,"abstract":"<div><div>The induced mechanisms underlying filamentous bulking by quorum sensing (QS) remain unclear. This study investigated how diffusible signal factors (DSF)-mediated QS facilitates filamentous bacterial proliferation via public goods cross-feeding. The results indicated that DSF concentrations increased by 4.32– and 5.03-fold in the aqueous and solid phases, respectively, correlating positively with the proliferation of the filamentous bacterium <em>Thiothrix</em> and upregulation of DSF-related genes (<em>rpfF</em>, <em>rpfC</em>, <em>rpfG</em>). Activation of the DSF-QS system stimulated threonine and serine biosynthesis and extracellular protease secretion, supporting the auxotrophic growth of <em>Thiothrix</em>. Correlation and phylogenetic analyses suggested that DSF-QS regulates the cross-feeding of these metabolites between <em>Thiothrix</em>, <em>Acinetobacter bohemicus</em> B and <em>Ca. Competibacter</em> B. Excessive proliferation of <em>Thiothrix</em> disrupted the physicochemical properties of sludge flocs and impaired aggregation, leading to sludge bulking. This study reveals that DSF-QS promotes filamentous bulking in activated sludge by regulating the cross-feeding of public goods.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132980"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newly discovered cyanobacteria Nostoc sp. PCC7120 for high efficiency biodegradation of thiamethoxam: Photosynthesis response, enzyme strategies, and molecular mechanisms 新发现的蓝细菌Nostoc sp. PCC7120高效生物降解噻虫嗪：光合作用响应、酶策略和分子机制

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132979

Shiye Zhu , Jiale Zhang , Anwei Chen , Youzheng Chai , Jianhua Zeng , Gen Lu , Ma Bai , Jihai Shao , Liang Peng , Si Luo

{"title":"Newly discovered cyanobacteria Nostoc sp. PCC7120 for high efficiency biodegradation of thiamethoxam: Photosynthesis response, enzyme strategies, and molecular mechanisms","authors":"Shiye Zhu , Jiale Zhang , Anwei Chen , Youzheng Chai , Jianhua Zeng , Gen Lu , Ma Bai , Jihai Shao , Liang Peng , Si Luo","doi":"10.1016/j.biortech.2025.132979","DOIUrl":"10.1016/j.biortech.2025.132979","url":null,"abstract":"<div><div>Agricultural expansion has led to the accumulation of neonicotinoid pesticides in water and soil, which are habitats for widespread cyanobacteria. However, the cyanobacteria-neonicotinoids interaction mechanisms remain unclear. This study first explores Thiamethoxam (THX) biodegradation by <em>Nostoc</em> sp. PCC7120, elucidating cytochrome P450 (CYP450)-mediated THX metabolism. <em>Nostoc</em> sp. PCC7120 could remove THX completely within 6 days mainly via biodegradation (75 %). While THX stimulates cell growth, evidence (damaged thylakoid membranes, lowered electron transfer efficiency, reduced photosynthetic pigments, and altered key gene expression) shows it disrupts photosynthesis. Furthermore, THX degradation involved ring opening, nitrate reduction, de-chlorination, and <em>N</em>-dealkylation; molecular dynamics simulations revealed THX stably binding (32.38 kcal/mol) to CYP450′s active site and primary degradation via hydroxylation and de-chlorination. Ecotoxicity assessments show desmethyl and urea metabolites are more toxic to non-target organisms and humans than the parent compound. This study elucidates THX removal mechanisms and environmental fate, highlighting cyanobacteria’s potential in bioremediation for agricultural wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132979"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated solvent extraction of high-purity lignin from sugarcane bagasse using sodium xylenesulfonate: Structural refinement and antibacterial-antioxidant enhancement. 二甲苯磺酸钠加速溶剂萃取蔗渣中高纯木质素：结构优化和抗菌抗氧化增强。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132988

Ekabhop Arunmas, Akkaratch Rodklongtan, Pakamon Chitprasert

{"title":"Accelerated solvent extraction of high-purity lignin from sugarcane bagasse using sodium xylenesulfonate: Structural refinement and antibacterial-antioxidant enhancement.","authors":"Ekabhop Arunmas, Akkaratch Rodklongtan, Pakamon Chitprasert","doi":"10.1016/j.biortech.2025.132988","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132988","url":null,"abstract":"<p><p>Conventional hydrotropic extraction often suffers from poor lignin-carbohydrate separation, high solvent use, and lengthy processing. To resolve these issues, this study applied accelerated solvent extraction (ASE) assisted by sodium xylenesulfonate (SXS). Three variables, SXS concentration (10 % w/v and 20 % w/v), extraction temperature (180 °C and 200 °C), and time (1  h and 3  h), were optimized to assess their effects on lignin extraction efficiency, purity, structure, and bioactivity. At 20 % SXS and 200 °C for 1  h, extraction efficiency peaked (66.18 %) but lignin purity was lowered by carbohydrate contamination (16.82 %). In comparison, the condition of 10 % SXS at 200 °C for 3  h provided a better balance, yielding lignin with high purity (97.61 %) despite its lower extraction efficiency (9.58 %), along with a lower weight-average molecular weight (M<sub>w</sub>: 2,100  Da) and extensive β-O-4 bond cleavage, with the lowest residual linkage content (8.82 per 100 aromatic units). An elevated syringyl-to-guaiacyl ratio reflected the preferential release of β-O-4-labile syringyl units. This lignin showed high phenolic (3.31 mmol/g) and carboxyl (0.59 mmol/g) contents. With ABTS and DPPH values of 1105.70 and 939.85 mg Trolox equivalent (TE)/g, respectively, the lignin showed strong antioxidant properties and inhibited pathogenic bacteria at MICs of 0.156-2.5 mg/mL. The MIC against probioticLimosilactobacillus reuteriKUB-AC5 was 1.25  mg/mL. Alkaline-extracted lignin had a higher molecular weight (4,100  Da) but showed reduced antioxidant activity (ABTS: 322.66 and DPPH: 274.26  mg TE/g) and higher MICs (1.25-10  mg/mL), indicating weaker antibacterial effects compared to optimized ASE-SXS lignin.These results affirm the functional advantages of lignin obtained via optimized ASE-SXS extraction.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132988"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-modulated algal-bacterial symbiosis: Dual detoxification and bioproduction enhancement from acid-lignocellulosic pretreatment wastewater 氮调节的藻-细菌共生：酸-木质纤维素预处理废水的双重解毒和生物生产增强。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132978

Huiying Wang , Wei Qi , Wen Wang , Mostafa Elshobary , Pingzhong Feng , Shunni Zhu , Zhongming Wang , Lei Qin

{"title":"N-modulated algal-bacterial symbiosis: Dual detoxification and bioproduction enhancement from acid-lignocellulosic pretreatment wastewater","authors":"Huiying Wang , Wei Qi , Wen Wang , Mostafa Elshobary , Pingzhong Feng , Shunni Zhu , Zhongming Wang , Lei Qin","doi":"10.1016/j.biortech.2025.132978","DOIUrl":"10.1016/j.biortech.2025.132978","url":null,"abstract":"<div><div>The lignocellulosic biorefining process produces acid-pretreated lignocellulosic wastewater (ALPW), which is rich in fermentable sugars but also contains inhibitory compounds (furfural/5-HMF/acetic acid). This study first systematically assessed the potential for ALPW valorization through algal-bacterial symbiosis with <em>N</em>-regulation. The results showed that 4 × ALPW enabled optimal algal biomass (3.95 g/L). Synchronous culture of algal-bacterial symbiosis in 2 × ALPW achieved 74.7 % sugar consumption and complete inhibitor detoxification, outperforming asynchronous culture. <em>N</em>-regulation with 80 mg/L NO<sub>3</sub><sup>−</sup>-N + 40 mg/L NH<sub>4</sub><sup>+</sup>-N maximized biomass (4.30 g/L) and carbon fixation (330.60 mg/L/d). The system converted 56.2 % carbon and 68.0 % nitrogen into biomass, with 1 ton corn stalk yielding 86.7 kg biomass (protein/carbohydrate/lipid: 36.6/27.1/16.0 kg). This research not only advances algal-bacterial wastewater treatment but also offers a practical, cost-effective pathway for lignocellulosic biorefineries to transition toward a circular economy. Future studies will prioritize pilot-scale validation of the <em>N</em>-modulated symbiosis for ALPW treatment and integration with biorefinery processes to improve economic viability.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132978"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Migration behavior of phosphorus during microwave-assisted co-hydrothermal carbonization and co-pyrolysis of chemical sludge and corncob 微波辅助化学污泥与玉米芯共热炭化共热解过程中磷的迁移行为

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132987

Baofeng Wang , Yunxiao Chen , Yaxin Guo , Mukun Xue , Huirong Zhang , Jinglei Cui , Jiawei Wang , Yanxia Guo

{"title":"Migration behavior of phosphorus during microwave-assisted co-hydrothermal carbonization and co-pyrolysis of chemical sludge and corncob","authors":"Baofeng Wang , Yunxiao Chen , Yaxin Guo , Mukun Xue , Huirong Zhang , Jinglei Cui , Jiawei Wang , Yanxia Guo","doi":"10.1016/j.biortech.2025.132987","DOIUrl":"10.1016/j.biortech.2025.132987","url":null,"abstract":"<div><div>Recycling of phosphorus (P) from chemical sludge (CS) is important for alleviating P resources shortage and solving P pollution. In this study, the transformation behavior and the mechanism of P during microwave-assisted co-hydrothermal carbonization (co-HTC) and co-pyrolysis of CS and corncob was investigated. The results showed that microwave could enhance the process of co-HTC and co-pyrolysis, and made P enriched in hydrochar and biochar. Microwaves made the content of orthophosphate (Ortho-P) and pyrophosphate (Pyro-P) in hydrochar and biochar increase, as well as making the organic phosphorus (OP) convert into inorganic phosphorus (IP). Meanwhile, the results also showed that P-containing minerals existed as Al-P and Fe-P in hydrochar and existed as Ca-P and Mg-P minerals in biochar. Furthermore, microwave assistance also could significantly improve the bioavailability of P. The results of this study may provide a basis for the P recovery from chemical sludge, as well as for clean and efficient utilization of hydrochar and biochar.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132987"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated hydrothermal and ball-milling approach for the co-production of glucose, xylose and residual lignin from Moso bamboo 水热和球磨联合生产毛竹中葡萄糖、木糖和残余木质素的研究。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-14 DOI: 10.1016/j.biortech.2025.132985

Qianying Yang , Chen Zhang , Lili Shang , Jialong Wen , Shumin Yang

{"title":"An integrated hydrothermal and ball-milling approach for the co-production of glucose, xylose and residual lignin from Moso bamboo","authors":"Qianying Yang , Chen Zhang , Lili Shang , Jialong Wen , Shumin Yang","doi":"10.1016/j.biortech.2025.132985","DOIUrl":"10.1016/j.biortech.2025.132985","url":null,"abstract":"<div><div>Clean and efficient pretreatment is critical for the sustainable development of bamboo industry. In this study, a combination of hydrothermal pretreatment (including AlCl<sub>3</sub> and glycerol) and ball-milling were proposed to efficiently facilitating co-production of xylose, glucose and residual lignin from moso bamboo. The results showed that the ball-milling process before hydrothermal pretreatment destroyed the compact structure of bamboo, and 44.5% xylose was obtained after hydrothermal pretreatment at 150 °C for 30 min. The 5 mins’ ball-milling process significantly reduced the “biomass recalcitrance” of the hydrothermal substrate. After 24 h of rapid enzymatic hydrolysis, the glucose yield increased from 32.6% to 81.3%, compared with 5.8% of the untreated bamboo. Finally, this method realized the effective isolation of enzymatic lignin with high-yield (87.0%), acceptable purity (84.3%) and non-condensed structure. In conclusion, the proposed strategy is a green, clean, and efficient strategy for the high-efficiency deconstruction and high-value utilization of bamboo resources.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132985"},"PeriodicalIF":9.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting 微生物介导的Fenton系统对秸秆堆肥过程中木质素转化的协同强化机制

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-13 DOI: 10.1016/j.biortech.2025.132981

Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei

{"title":"Synergistic strengthening mechanism of microbial-mediated Fenton system on lignin depolymerization during rice straw composting","authors":"Fengting Qu , Li Zhao , Yinan Cao , Taha Ahmed Mohamed , Zimin Wei","doi":"10.1016/j.biortech.2025.132981","DOIUrl":"10.1016/j.biortech.2025.132981","url":null,"abstract":"<div><div>This study investigated the synergistic strengthening mechanism of a microbial-mediated Fenton system in lignin depolymerization during composting. The Fenton system was constructed using hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced by lignin-degrading microorganisms along with Fe(Ⅱ). The simultaneous inoculation of bacteria and fungi resulted in optimal enzyme activities related to lignocellulose degradation and lignin degradation rates. Moreover, the addition of 0.5% FeSO<sub>4</sub> further optimized hydroxyl radical (<strong>·</strong>OH) production. Therefore, four treatments were set: CK (control), BHF (bacterial fungi synchronized inoculation), Fe (FeSO<sub>4</sub>), and BHF-Fe (bacterial fungi synchronized inoculation + FeSO<sub>4</sub>). BHF-Fe synergistically drove the Fe(Ⅱ)/Fe(Ⅲ) redox cycle and maintained reactive oxygen species ROS, (superoxide anion, H<sub>2</sub>O<sub>2</sub> and <strong>·</strong>OH) generation. BHF-Fe showed a 22.58% greater lignin loss rate compared to the CK. The correlations between ROS and lignin loss rate were enhanced. Additionally, lignin peroxidase, as an extracellular enzyme, relies on H<sub>2</sub>O<sub>2</sub> for its action on lignin. The activities of laccase and lignin peroxidase were significantly correlated. Lignin decomposition was accelerated through the upregulation of key degrading enzyme genes (<em>Lac</em>, <em>LiP</em>, and <em>MnP</em>). The Fenton system showed attenuated pH dependence relative to conventional systems despite pH and Fe (II) effects on ROS. The BHF-Fe achieves efficient lignin degradation through a synergistic mechanism that integrates Fenton reactions, ROS, biocatalytic enzymatic oxidation and microenvironment compensation. This research offers theoretical and technical backing for developing agricultural waste resource recycling and composting technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132981"},"PeriodicalIF":9.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0