Bioresource Technology最新文献_第3页

Foam formation during anaerobic digestion of sugar beet silage: causes and countermeasures. 甜菜青贮厌氧消化过程中泡沫的形成：原因及对策。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-19 DOI: 10.1016/j.biortech.2025.133180

Frederik Bade, Sabine Kleinsteuber, Lucie Moeller

引用次数: 0

Mechanistic insights into nitrogen-mediated carbon transformation during hydrothermal humification for the synthesis of nutrient-enriched artificial humic acids. 热液腐殖酸合成过程中氮介导碳转化的机理。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.biortech.2025.133100

Dan Wang, Ziyun Liu, Jinting Su, Yanan Zhao, Lixin Zhao, Lili Huo, Zonglu Yao

{"title":"Mechanistic insights into nitrogen-mediated carbon transformation during hydrothermal humification for the synthesis of nutrient-enriched artificial humic acids.","authors":"Dan Wang, Ziyun Liu, Jinting Su, Yanan Zhao, Lixin Zhao, Lili Huo, Zonglu Yao","doi":"10.1016/j.biortech.2025.133100","DOIUrl":"10.1016/j.biortech.2025.133100","url":null,"abstract":"Hydrothermal humification (HTH) of biomass is a promising approach to address the depletion of soil organic matter. However, the HTH products derived from lignocellulose are limited by low nutrient availability and conversion efficiency. In this study, four nitrogen-containing additives were used to produce nitrogen-rich artificial humic acids (AHA) from crop waste. The addition of urea, ammonium chloride, ammonium sulfate, and ammonium dihydrogen phosphate at 180 °C for 1 h significantly improved both the yield and quality of AHA. Optimal conditions were achieved using ammonium sulfate at a carbon-to-nitrogen ratio of 5, resulting in an impressive AHA yield of 40.75 ± 3.99 wt% and a nitrogen content of 3.54 %, closely resembling naturally formed humic acids. This represented a 38.27 % increase in yield and a 30.44 % increase in nitrogen content compared to the control. Moreover, 5NS-AHA exhibited reduced aromaticity, increased oxygen-containing functional groups, and enhanced functionality through the regulation of aliphatic and aromatic carbon structures. The concentration of furfural in the hydrothermal aqueous phase reduced from 43.48 % to 29.85 %. Ammonium sulfate optimized the HTH pathway through synergistic mechanisms involving acid-catalyzed oxidation, nitrogenated condensation, and aromatic condensation. This work provides a visible pathway for producing nitrogen slow-release AHA fertilizers from agricultural waste.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133100"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ visual delignification and cell-scale kinetics modeling using confocal Raman microscopy. 使用共聚焦拉曼显微镜进行原位视觉脱木质素和细胞尺度动力学建模。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.biortech.2025.133098

Yuanping Huang, Zengling Yang, Chenjun Ge, Zhuolin Shi, Xinlei Wang, Lujia Han

{"title":"In-situ visual delignification and cell-scale kinetics modeling using confocal Raman microscopy.","authors":"Yuanping Huang, Zengling Yang, Chenjun Ge, Zhuolin Shi, Xinlei Wang, Lujia Han","doi":"10.1016/j.biortech.2025.133098","DOIUrl":"10.1016/j.biortech.2025.133098","url":null,"abstract":"Efficient delignification remains a critical technological bottleneck hindering the valorization of plant cell wall resources. Plant cell walls are dynamic, spatially heterogeneous networks characterized by a highly interconnected physical architecture and complex chemical composition. In-situ visualization of delignification dynamics at the cell scale, coupled with a quantitative understanding of cell-specific delignification kinetics, provide a powerful approach for a deeper understanding of the delignification process. In this study, Confocal Raman microscopy mapping was employed to obtain lignin content and visualize the in-situ delignification process in different tissues and cells from rice stem, including parenchyma, sclerenchyma, protoxylem, vascular bundle sheath, epidermis, metaxylem, and external vascular bundle. Results revealed pronounced cell-type-dependent delignification responses, further supporting the concept that recalcitrance is not solely determined by lignin content per se. Furthermore, this study established, for the first time, the delignification kinetic models for these distinct tissues and cells, providing a quantitative framework for describing lignin content dynamics during acidified sodium chlorite delignification process. This study offers a valuable approach for understanding and optimizing cell-type-specific responses during the implementation of delignification strategies.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133098"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming agri-food waste into value: Sustainable approaches for next-generation resistant starch production. 将农业食品垃圾转化为价值：下一代抗性淀粉生产的可持续方法。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133090

Shikha Pandhi, Dipendra Kumar Mahato, Anju Kurian, Surabhi Pandey, Abhishek Thamarai, Vishwas Gaur, Madhu Kamle, Amritesh Chandra Shukla, Pradeep Kumar

{"title":"Transforming agri-food waste into value: Sustainable approaches for next-generation resistant starch production.","authors":"Shikha Pandhi, Dipendra Kumar Mahato, Anju Kurian, Surabhi Pandey, Abhishek Thamarai, Vishwas Gaur, Madhu Kamle, Amritesh Chandra Shukla, Pradeep Kumar","doi":"10.1016/j.biortech.2025.133090","DOIUrl":"10.1016/j.biortech.2025.133090","url":null,"abstract":"Agri-food waste, which includes organic materials discarded throughout the food supply chain from agricultural production and post-harvest handling to processing, distribution, and consumption, poses significant environmental and economic challenges due to resource underutilization and disposal issues. However, the valorization of these residues into value-added products, particularly resistant starch (RS), offers a sustainable solution. RS, a non-digestible carbohydrate with prebiotic properties, provides numerous health benefits, including improved gut health, blood sugar regulation, and enhanced satiety. Various studies have explored innovative methods for RS extraction from agricultural by-products such as potato peels, rice bran, banana peels, and chestnut starch. Techniques such as enzymatic hydrolysis, ultrasound-assisted extraction, and thermal processing have demonstrated high efficiency in producing RS while maintaining its functional properties. Additionally, advancements in artificial intelligence (AI), big data, and machine learning are playing a crucial role in optimizing waste valorization processes. AI-driven technologies enable precise classification and separation of food waste, improving the efficiency of extraction and processing techniques. The integration of smart systems, such as IoT-based waste classification, further enhances resource recovery. As research and technological advancements continue, the large-scale application of these sustainable extraction and processing methods is expected to support a circular economy, reduce food waste, and provide functional food ingredients for the food industry.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133090"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting biomass gasification products for bubbling fluidised beds using high order polynomial regression with regularisation: a simple but highly effective strategy. 用正则化的高阶多项式回归预测鼓泡流化床生物质气化产物：一种简单但高效的策略。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133109

Michael Binns

{"title":"Predicting biomass gasification products for bubbling fluidised beds using high order polynomial regression with regularisation: a simple but highly effective strategy.","authors":"Michael Binns","doi":"10.1016/j.biortech.2025.133109","DOIUrl":"10.1016/j.biortech.2025.133109","url":null,"abstract":"For the design of biomass gasification it is beneficial to have models which can predict the composition of gas products for a wide range of different biomass feedstocks. Complex machine learning models (e.g. neural networks and tree-based methods) are now being used for this purpose which are difficult to reproduce with large numbers of parameters involved. In this study the potential for higher order polynomials is investigated for the modelling of bubbling fluidised bed gasification. To reduce the number of parameters and to avoid over-fitting Least Absolute Shrinkage and Selection Operator (LASSO) regularisation is used. This is a novel application of high order polynomial regression with regularisation which allows the prediction of hydrogen composition with coefficient of performance of 0.9228 and only 85 fitted parameters. The next best existing methods give coefficients of performance of 0.8823 and 0.868 but require 261 parameters and more than 1000 parameters respectively. So this polynomial approach is shown to give accurate model prediction with simpler model equations.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133109"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coupling mechanisms of community assembly and pollutant removal in algal-bacterial granular sludge systems. 藻-细菌颗粒污泥系统中群落聚集与污染物去除的耦合机制。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biortech.2025.133122

Rushuo Yang, Zhe Liu, Yongjun Liu, Zhuangzhuang Yang, Zhu Wang, Yuhang Zhang, Jie Lei, Tianyu Han, Jiaxuan Wang, Zhihua Li

{"title":"Coupling mechanisms of community assembly and pollutant removal in algal-bacterial granular sludge systems.","authors":"Rushuo Yang, Zhe Liu, Yongjun Liu, Zhuangzhuang Yang, Zhu Wang, Yuhang Zhang, Jie Lei, Tianyu Han, Jiaxuan Wang, Zhihua Li","doi":"10.1016/j.biortech.2025.133122","DOIUrl":"10.1016/j.biortech.2025.133122","url":null,"abstract":"This study comparatively assessed algal-bacterial granular sludge (ABGS) decontamination performance and microbial community mechanisms under two cultivation modes: flocculated sludge transformed into ABGS (AS_ABGS) and aerobic granular sludge transformed into ABGS (AGS_ABGS). The results indicated that, AS_ABGS achieved superior pollutant removal (COD: 92.2 %, TN: 82.1 %, TP: 61.5 %) versus AGS_ABGS (COD: 96.3 %, TN: 75.1 %, TP: 53.3 %). Microbial community analysis revealed deterministic assembly dominated AS_ABGS, narrowing niches, and enhancing functional specialization of N/P-removing taxa. AS_ABGS exhibited higher modularity, robustness, and stronger positive algal-bacterial interactions (52.32 %). Conversely, stochastic assembly in AGS_ABGS yielded weaker interactions (50.45 %). Metagenomics confirmed AS_ABGS enriched N/P metabolic genes (amo, acc) may be driven by Thauera, Micavibrio, and Aquisediminimonas, while AGS_ABGS favored Amaricoccus and Rhodovulum but showed lower N/P gene abundance. This study highlights the effect of algal-bacterial ecological interactions mediated by functional genes on the decontamination efficiency of ABGS and provides valuable insights for advancing this method.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133122"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing biochar quality for the steel industry via Hydrothermal Pretreatment-Steam Explosion and pyrolysis. 通过水热预处理-蒸汽爆炸和热解提高钢铁工业生物炭的质量。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133009

Chay A Davies-Smith, Julian Herbert, Ciarán Martin, Darbaz Khasraw, David Warren-Walker, David Bryant, Joe Gallagher, Gordon Allison, Julian M Steer, Richard Marsh, Ahmed Alsawadi, Rakesh Bhatia

{"title":"Enhancing biochar quality for the steel industry via Hydrothermal Pretreatment-Steam Explosion and pyrolysis.","authors":"Chay A Davies-Smith, Julian Herbert, Ciarán Martin, Darbaz Khasraw, David Warren-Walker, David Bryant, Joe Gallagher, Gordon Allison, Julian M Steer, Richard Marsh, Ahmed Alsawadi, Rakesh Bhatia","doi":"10.1016/j.biortech.2025.133009","DOIUrl":"10.1016/j.biortech.2025.133009","url":null,"abstract":"Biochar has potential applications in steelmaking processes, but faces technical challenges such as low material density, high alkali content, and high reactivity compared to coal. This study explores converting the solid residue, following hydrothermal pretreatment-steam explosion (HTP-SE) of Miscanthus and other biomass feedstocks, into biochar to facilitate the replacement of coal in blast furnace and electric arc furnace operations. It is the first to demonstrate the enhanced combustion characteristics of pretreated fibre and the compatibility of the biochar for use in steelmaking. Biomass from birch, miscanthus, wheat straw, both untreated and pretreated, was evaluated. HTP-SE was conducted at 192 °C and 1.3 MPa, conditions aligned with hemicellulose extraction for application in biobased products. Biochars were produced at temperatures ranging from 300 °C to 550 °C. HTP-SE increased the carbon, hydrogen, and energy content by approximately 10%, 8%, and up to 5 MJ/kg, respectively, while reducing ash quantity by up to 45%. In addition, it reduced the alkali and phosphorus content from the solid fraction into aqueous phase. Gas analysis indicated that HTP-SE enhanced the energy content of pyrolysis syngas. Thermogravimetric studies revealed that pretreated biochars exhibited significantly lower reactivity with carbon dioxide compared to untreated counterparts, approaching the reactivity of coal. This was attributed to increased aromaticity, C=C bonding, cross-linkages enriching lignin and by the removal of hemicellulose through HTP-SE. Overall, the upgraded biochar addresses key limitations of conventional biochar and shows strong potential as a substitute to replace injection coal entirely in both blast and electric arc furnaces.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133009"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of denitrification by MnO₂-modified biochar under low-temperature: Performance evaluation and mechanistic insights. 低温条件下mno2改性生物炭增强反硝化作用：性能评价和机理研究。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biortech.2025.133073

Hao Qin, Min Nie, Fucheng Guo, Tao Liu, Gongliang Zhang, Yi Chen

{"title":"Enhancement of denitrification by MnO2-modified biochar under low-temperature: Performance evaluation and mechanistic insights.","authors":"Hao Qin, Min Nie, Fucheng Guo, Tao Liu, Gongliang Zhang, Yi Chen","doi":"10.1016/j.biortech.2025.133073","DOIUrl":"10.1016/j.biortech.2025.133073","url":null,"abstract":"Low temperatures significantly hinder denitrification in cold climates. In this study, MnO2-modified biochar (MBC) was utilized to improve microbial denitrification at low temperatures. Results showed that the modification considerably raised the specific surface area, pore volume, and electron transfer capacity of biochar (BC) by 185%, 88%, and 30%, respectively. MBC significantly enhanced the TN removal efficiency of denitrifiers, reaching 3.23 mgN·gVSS-1·h-1. The activities of key enzymes linked to glycolysis (HK, PFK, PK) and denitrification (Nar, Nir, Nos), alongside the electron transport system activity, as well as ATP and NADH levels, were significantly enhanced. The enrichment of Acidovorax and Tolumonas indicated that microbial manganese redox cycling played a critical role in promoting TN removal. Column experiment further demonstrated that MBC improved the TN removal efficiency of the denitrification biofilter by 33%, highlighting its practical efficacy in biofilm systems. Overall, MBC presents a novel strategy for enhancing denitrification efficiency at low temperatures.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133073"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the inhibition effect of hemicellulosic sugars on the enzymatic hydrolysis of cellulose: A combined experimental and computational study. 了解半纤维素糖对纤维素酶解的抑制作用：实验和计算相结合的研究。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-07-31 DOI: 10.1016/j.biortech.2025.133060

Xiaoxiao Jiang, Tan Lai Heng, Yuguang Mu, Rui Zhai, Mingjie Jin

{"title":"Understanding the inhibition effect of hemicellulosic sugars on the enzymatic hydrolysis of cellulose: A combined experimental and computational study.","authors":"Xiaoxiao Jiang, Tan Lai Heng, Yuguang Mu, Rui Zhai, Mingjie Jin","doi":"10.1016/j.biortech.2025.133060","DOIUrl":"10.1016/j.biortech.2025.133060","url":null,"abstract":"Sugar-platform biorefinery, involving pretreatment and enzymatic hydrolysis, is the central part of efficient utilization of lignocellulosic biomass. However, hemicellulose-derived sugars generated during pretreatment impose specific inhibition on enzymatic hydrolysis of cellulose. With the growing industrial demand for complete utilization of fermentable sugars, the inhibition by high concentration of hemicellulosic sugars represents a major challenge for economically viable biorefinery. This study initially investigated the inhibition degree of hemicellulosic sugars (xylose and mannose) on the enzymatic hydrolysis of cellulose, revealing that the inhibition by hemicellulosic sugars was mainly affected by the hydrolysis of crystalline cellulose and further determined by the hydrolytic performance of CBHI. Then, the inhibition effect of hemicellulosic sugars on CBHI kinetics was analyzed by combining interfacial experiment and theoretical model, demonstrating that the inhibition on productive association and processive movement was the determining factors affecting the hydrolytic performance of CBHI. Further, molecular dynamic simulations were performed and suggested that xylose binds with CBHI steadily in its substrate-binding tunnel through hydrogen bonds and hydrophobic interactions, accounting for the impaired productive association and processive movement. Our study provides improved insight into the inhibition mechanism by hemicellulosic sugars, and offers avenues for engineering more efficient cellulase and achieving more economically viable biorefinery.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133060"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sludge reflux promotes biofilm formation in upflow membrane aerated biofilm reactor for partial nitritation: insight from computational fluid dynamics stimulation and experiments. 污泥回流促进部分硝化上流式膜曝气生物膜反应器中生物膜的形成：来自计算流体动力学刺激和实验的见解。

IF 9 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI: 10.1016/j.biortech.2025.133077

Jinlong Zhuang, Wei Ai, Qingheng Wang, Huijuan Wang, Bingdang Wu, Xiaoyi Xu, Tianyin Huang

{"title":"Sludge reflux promotes biofilm formation in upflow membrane aerated biofilm reactor for partial nitritation: insight from computational fluid dynamics stimulation and experiments.","authors":"Jinlong Zhuang, Wei Ai, Qingheng Wang, Huijuan Wang, Bingdang Wu, Xiaoyi Xu, Tianyin Huang","doi":"10.1016/j.biortech.2025.133077","DOIUrl":"10.1016/j.biortech.2025.133077","url":null,"abstract":"Achieving partial nitritation (PN) process using membrane aerated biofilm reactor (MABR) is a novel approach to provide nitrite for mainstream anammox, gained increasing interests. However, insufficient microbial-membrane attachment raises a challenge for biofilm formation in MABR-PN. This study employed computational fluid dynamics modeling to evaluate sludge volume fraction in biofilm formation zones, using liquid reflux and sludge reflux as comparative strategies for enhancing biomass attachment. The optimal performance was obtained in sludge reflux (5 min/4 h), with 40 % sludge volume fraction and the uniformity of sludge distribution in the biofilm formation zone was 80 %. Two bench-scale MABR were developed with liquid reflux (R1:400 % reflux) and sludge reflux (R2:5 min/4 h) under influent NH4+-N concentration of 50 ± 2 mg/L. The biofilm formation time in R2 was significantly shortened 30 % compared to R1, and the nitrite accumulation rate of R2 was significantly higher than that of R1 by 18.5 %. 16S rRNA gene sequencing coupled with observation revealed that the extracellular polymeric substance (EPS)-producing microorganism was enriched in biofilm of R2, meanwhile the abundance of the Nitrosomonas in R2_biofilm was significantly higher than flocs. Moreover, in R2, the abundance of key synthesis genes for EPS in biofilm was 2.4-fold higher than in flocs. The EPS concentration determination showed that R2_biofilm > R1_flocs > R1_biofilm > R2_flocs, confirmed that the high EPS-producing capacity in R2 biofilms could promote biofilm formation. Sludge reflux could promote the formation of MABR biofilm and enable rapid start-up of the PN process. Additionally, the energy consumption of R2 was only 2.5 % of R1.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"133077"},"PeriodicalIF":9.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0