{"title":"Production of polyhydroxyalkanoate from new isolated bacteria of Acidovorax diaphorobacter ZCH-15 using orange peel and its underlying metabolic mechanisms.","authors":"Jinju Hou, Lei Cheng, Shudong Zhang, Xiaotong Zhang, Xilong Zheng, Qiuzhuo Zhang","doi":"10.1016/j.biortech.2024.131949","DOIUrl":"10.1016/j.biortech.2024.131949","url":null,"abstract":"<p><p>Polyhydroxyalkanoate (PHA) is considered a sustainable alternative to traditional petroleum-based plastics due to its biodegradability and biocompatibility. In this study, Acidovorax diaphorobacter ZCH-15, an efficient PHA-producing strain, was isolated from activated sludge. Using food waste-derived orange peel as a substrate, the strain initially achieved a PHA concentration of 0.39 g/L. Under optimal fermentation conditions (30℃, pH 8, 2 % inoculum concentration, and 30 g/L carbon source), the PHA concentration increased by 138 % to reach a maximum of 0.93 g/L. Proton nuclear magnetic resonance spectroscopy and gas chromatography analyses identified the PHA composition as poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which exhibited high crystallinity and structural stability. Metabolomic analysis indicated that the tricarboxylic acid cycle and pentose phosphate pathway were involved in producing succinyl-CoA, a precursor required for PHA synthesis. This study demonstrates the potential for cost-effective industrial PHA production while enabling the high-value utilization of food waste.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131949"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Weak interaction strategy enables enhanced selectivity and reusability of arginine-functionalized imprinted aerogel for phosphate adsorption.","authors":"Lidong Feng, Tianxiao Leng, Yangbo Qiu, Chao Wang, Long-Fei Ren, Haoyu Sun, Liang Tang, Jiahui Shao, Minghong Wu","doi":"10.1016/j.biortech.2024.131960","DOIUrl":"10.1016/j.biortech.2024.131960","url":null,"abstract":"<p><p>Efficient phosphate adsorption from eutrophic waters remains challenging, fundamentally due to inherent trade-off in common adsorbents: high-binding energy between adsorbent and phosphate compromises reusability while low-binding energy suppresses selectivity. Herein, an innovative arginine-functionalized imprinted aerogel (AFIA-1:4) was fabricated by click chemistry and imprinting modification for overcoming this trade-off through synergistic weak interactions. Results shown that AFIA-1:4 exhibited high adsorption capacity (Q<sub>max</sub> of 40.65 mg/g, 30.44 % higher than phoslock), rapid kinetics (15 min), and broad pH applicability (3-11) at 2 mg P/L solution. Moreover, its selectivity coefficient ranged from 10 to 90 even with 15- to 125-fold excess interfering anions, surpassing common adsorbents. After 10 cycles, AFIA-1:4 still maintained 98.15 % regeneration rate with 99.14 % phosphate desorption. Characterizations and calculations confirmed core roles of multiple hydrogen bonds and shape screening in maintaining selectivity and reusability. These findings advanced development of next-generation of phosphate adsorbents, which contributed to sustainable prevention and management of eutrophication.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131960"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxidative pyrolysis of alkali lignin using nitrogen functionalized graphene oxide-cerium oxide nanocatalysts: Mechanistic insights thorough density functional theory.","authors":"Shivam Kumar, Pankaj Kumar, Navneet Kumar, Jinsub Park, Vimal Chandra Srivastava","doi":"10.1016/j.biortech.2024.131985","DOIUrl":"10.1016/j.biortech.2024.131985","url":null,"abstract":"<p><p>In this study, a functionalized graphene oxide-cerium oxide nanocatalysts (FGCe) with varying graphene oxide (GO) contents were prepared using an in-situ reflux method. The prepared nanocatalysts showcased improvement in the crystallinity and BET surface area values with increasing GO contents. The efficacies of prepared catalysts were investigated towards oxidative pyrolysis of alkali lignin in an ethanol-water system. Among various nanocatalyst samples, the best lignin conversion (93 %) and bio-oil yield (86 %) were achieved using 50 mg FGCe nanocatalyst (0.5 wt% GO) at 423 K and 60 min. GC-MS and <sup>1</sup>HNMR analyses were used to identify significant lignin conversion products, including 2-pentanone-4-hydroxy-4-methyl, 2-methoxyphenol, nonylcyclopropane, vanillin, apocynin, homovanollic acid, and benzoic acid. Kinetic studies revealed that the activation energy for lignin conversion was 24.36 kJ/mol at 423 K. Mechanistic investigations by density functional theory analysis revealed that the lignin breakdown occurred at oxygen bonds producing aromatic.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131985"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2025-02-01Epub Date: 2024-11-26DOI: 10.1016/j.biortech.2024.131892
Hyo Jik Yoon, Jeong Seop Lee, Ki Ha Min, Dong Hyun Kim, Sang Jun Sim, Seung Pil Pack
{"title":"Economic and demonstrative pilot-scale harvesting of microalgae biomass via novel combined process of dissolved air flotation and screw-press filtration.","authors":"Hyo Jik Yoon, Jeong Seop Lee, Ki Ha Min, Dong Hyun Kim, Sang Jun Sim, Seung Pil Pack","doi":"10.1016/j.biortech.2024.131892","DOIUrl":"10.1016/j.biortech.2024.131892","url":null,"abstract":"<p><p>Microalgae, a promising sustainable biomass resource, lacks sufficient research for pilot-scale processes despite available technologies. Harvesting methods also pose challenges for large-scale applications. To address this, the economically viable large-scale microalgae harvesting system is here presented. The design integrates dissolved air flotation (5 m<sup>3</sup>/h) and screw-press filtration (10 kg/h), minimizing energy consumption suitable for industrial processes. This system efficiently harvests chlorella sp. (up to 4.1 m<sup>3</sup>) with a biomass harvest efficiency of 93 % and a dewatering rate of 11.9 %. Compared to centrifugation, the multi-stage system improves energy efficiency by 60.5 % with 1.7 kWh/m<sup>3</sup> of energy consumption. This innovative approach demonstrates the potential for large-scale microalgae biomass harvesting.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131892"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biofilm-based immobilized fermentation of engineered Komagataella phaffii for xylanase production.","authors":"Huanqing Niu, Daoguang Zhu, Jing Leng, Zhenyu Wang, Dong Liu, Yong Chen, Pengpeng Yang, Hanjie Ying","doi":"10.1016/j.biortech.2024.131918","DOIUrl":"10.1016/j.biortech.2024.131918","url":null,"abstract":"<p><p>This study presented an immobilized fermentation process of engineered Komagataella phaffii with improved biofilm-forming abilities for continuous xylanase production and provided the first insights into the molecular basis of biofilm-based immobilized fermentation of K. phaffii. Overexpression of PAS_chr2-2_0178 and PAS_FragB_0067 in K. phaffii facilitated biofilm formation with 31.6% and 113.8% increasement, respectively. Subsequently, a biofilm-based immobilized fermentation process was developed for the PAS_FragB_0067-overexpressing strain. Xylanase production over five batches by GS115-0067* was better than that of GS115-xyn, with an overall average of 35.4 % higher enzyme activity. PAS_FragB_0067 overexpression resulted in better adhesion of K. phaffii cells on the carrier, and enhanced biofilms could provide more active cells in the immobilized fermentation process. Transcriptome analysis revealed that overexpression of the biofilm-related gene promoted central carbon metabolism. These findings offer a valuable reference strategy to improve production efficiency of K. phaffii cells in continuous fermentation processes.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131918"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2025-02-01Epub Date: 2024-12-16DOI: 10.1016/j.biortech.2024.131995
Jiaxin Sun, Tanlong Zhou, Fubin Yin, Shunli Wang
{"title":"Anaerobic co-biodegradation of polyhydroxyalkanoate and swine manure for volatile fatty acid production: The impact of C/N ratios and microbial dynamics.","authors":"Jiaxin Sun, Tanlong Zhou, Fubin Yin, Shunli Wang","doi":"10.1016/j.biortech.2024.131995","DOIUrl":"10.1016/j.biortech.2024.131995","url":null,"abstract":"<p><p>Polyhydroxyalkanoate (PHA) is the important biodegradable plastic, however, biodegradation of PHA waste in anaerobic environments emits more CH<sub>4</sub>, a potent greenhouse gas. Bioconversion of PHA waste to useful byproducts - volatile fatty acids (VFAs) is a practical method to upcycle carbon from PHA. In this study, PHA waste was anaerobically co-digested with swine manure (SM) (the typical high nitrogen waste) at different C/N ratios. The results indicate that co-digestion of PHA and SM with a C/N ratio of 32.1 achieved VFA production of 5488 mg COD/L and 0.20 g COD/g VS. No significant differences were found in terms of the highest VFA concentrations between treatments with C/N ratios of 43.4 and 32.1. VFA produciton of 3655 mg COD/L and 0.14 g COD/g VS was achieved at 19 days by adjusting the C/N ratio to 19.2. Four bacteria were identified as dominant microorganisms responsible for converting PHA and SM to VFA.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131995"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2025-02-01Epub Date: 2024-12-18DOI: 10.1016/j.biortech.2024.131999
Baorui Zhang, Jianbo Liu, Chen Cai, Yan Zhou
{"title":"Membrane photobioreactor for biogas capture and conversion - Enhanced microbial interaction in biofilm.","authors":"Baorui Zhang, Jianbo Liu, Chen Cai, Yan Zhou","doi":"10.1016/j.biortech.2024.131999","DOIUrl":"10.1016/j.biortech.2024.131999","url":null,"abstract":"<p><p>The urgency to mitigate greenhouse gas emissions has driven interest in sustainable biogas utilization. This study investigates a 1 L enclosed membrane photobioreactor (MPBR) using a microalgae-methanotroph coculture for biogas capture. Operating with a hydraulic and solid retention time of 7 days and a biogas loading rate of 2.7 L /day, the introduction of gas membrane module increased CO<sub>2</sub>-C and CH<sub>4</sub>-C uptake rates by 12 % and 50 %, respectively. Biofilm formation on the membrane surface enhanced system performance, with imaging analyses revealing methanotroph predominantly located near the membrane surface and photosynthetic microorganisms distributed throughout. Metagenomic analysis showed shifts in key metabolic pathways, including increased abundance of soluble methane monooxygenase genes and enhanced vitamin B synthesis in the biofilm. These findings highlight the spatial organization and metabolic interactions in methanotroph-microalgae coculture system, providing insights into the role of membrane-induced biofilms in improving MPBR performance for sustainable biogas utilization.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131999"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Should releasing risk of sulfamethoxazole in bound-residual fraction of biochar-soil systems be neglected?","authors":"Mingyang Sun, Xiaoyue Zhang, Jiaping Li, Yuyu Hu, Dehao Guo, Guanghuan Cheng","doi":"10.1016/j.biortech.2024.131921","DOIUrl":"10.1016/j.biortech.2024.131921","url":null,"abstract":"<p><p>Bound residual (BR) fraction typically represents the \"endpoint\" of soil pollution risk assessment and remediation. However, it remains unclear whether the BR fraction of sulfamethoxazole (SMX) still presents a release risk after immobilized by biochar. Therefore, the SMX migration rate in different fractions were quantified. Results revealed that there are three extractable fractions of SMX in biochar-soil systems, allowing the migration of SMX among these various fractions. 1.0 % biochar could increase 8.5 %∼14.1 % BR fraction and 17.9 %∼36.9 % non-extractable fraction, respectively. Model simulations and Sobol Global Sensitivity Analysis results revealed that migration of SMX from BR fraction to labile fraction or stably-adsorbed fraction significantly affects overall fraction migration, suggesting BR fraction still poses a potential release risk. When considering the BR fraction, the half-life of SMX extended to 93.8 ∼ 132.0 days. Thus, the potential release risk of SMX in the BR fraction should not be overlooked when applying biochar for soil remediation.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131921"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2025-02-01Epub Date: 2024-12-02DOI: 10.1016/j.biortech.2024.131920
Huaiyu Duan, Xinyi Zhang, Xingqing Zhao, Chao Xu, Du Zhang, Wei Gu, Rucheng Wang, Xiancai Lu
{"title":"Study on biogenic acid-mediated enhanced leaching of lepidolite by Aspergillus niger based on transcriptomics.","authors":"Huaiyu Duan, Xinyi Zhang, Xingqing Zhao, Chao Xu, Du Zhang, Wei Gu, Rucheng Wang, Xiancai Lu","doi":"10.1016/j.biortech.2024.131920","DOIUrl":"10.1016/j.biortech.2024.131920","url":null,"abstract":"<p><p>The synergistic effect between microorganisms with different modes of action can improve the leaching efficiency. In this study, biogenic acid was extracted from bacterial metabolites to induce fungal secretion, strengthen fungal leaching, and reveal the microscopic mechanism of biogenic acid stimulating fungal metabolic synthesis through transcriptome analysis. The results showed that the addition of biogenic acid increased the biomass and metabolic activity of Aspergillus Niger, changed the secretion of extracellular polymeric substances, enhanced the dissolution of mineral surface, and increased the leaching rate. A total of 3556 up-regulated and 2225 down-regulated differential genes were identified in Aspergillus Niger spores by transcriptome analysis after the addition of biogenic acid, with significant functional enrichment mainly related to organic acid metabolism. These findings suggest that biogenic acid have a beneficial catalytic effect on Aspergillus Niger, providing understanding and support for the interaction mechanism between different strains of combined leaching.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131920"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bioresource TechnologyPub Date : 2025-02-01Epub Date: 2024-12-04DOI: 10.1016/j.biortech.2024.131939
Tingting Liu, Ran Guo, Xinyi Wang, Ning Gu, Na Wu, Jianguo Wu, Yuxian Wang
{"title":"Enhanced butanol production through intracellular NADH regeneration in CdSe-C. acetobutylicum<sub>g</sub> semi-photosynthetic biohybrid system.","authors":"Tingting Liu, Ran Guo, Xinyi Wang, Ning Gu, Na Wu, Jianguo Wu, Yuxian Wang","doi":"10.1016/j.biortech.2024.131939","DOIUrl":"10.1016/j.biortech.2024.131939","url":null,"abstract":"<p><p>Current environmental challenges and energy crises highlight the urgent need for a transition in energy mix. In this study, an innovative semi-photosynthetic biohybrid system that combined light-activated cadmium selenide quantum dots (CdSe QDs) with engineered Gram-positive anaerobic bacteria, Clostridium acetobutylicum<sub>g</sub> (C. acetobutylicum<sub>g</sub>), was developed to enhance renewable butanol production. The results demonstrated that CdSe QDs could be biosynthesized intracellularly within C. acetobutylicum<sub>g</sub> through the introduction of glutathione pathway, without causing significant damage to bacteria. Furthermore, this system showed remarkable tolerance to butanol and weak acids. Under illumination, the biological synthesized CdSe QDs enabled C. acetobutylicum<sub>g</sub> to achieve a 45.5 % increase in NADH/NAD<sup>+</sup> ratio compared to C. acetobutylicum<sub>g</sub> without CdSe QDs. When utilizing undetoxified rice straw hydrolysate in photo-fermentation, this system achieved a butanol titer of 14.82 g/L and a yield of 0.29 g/g. Overall, this work aims to effectively harness solar energy and biomass resources for sustainable clean biofuel production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131939"},"PeriodicalIF":9.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}