Bioresource Technology最新文献

{"title":"Integrated metagenomic and metaproteomic insights into current-carrying-coil magnetic field enhanced synergistic methanogenic system and antibiotic resistance gene reduction in cow manure anaerobic digestion.","authors":"Rouf Ahmad Dar, To-Hung Tsui, Zhaoxiang Du, Le Zhang, Adam Smoliński, Guanning Xiang, Ronghou Liu","doi":"10.1016/j.biortech.2026.135326","DOIUrl":"10.1016/j.biortech.2026.135326","url":null,"abstract":"<p><p>Anaerobic digestion (AD) is a sustainable strategy for valorizing cow manure (CM). However, the high ammonia (NH₃) concentration and low biodegradability of CM limit hydrolysis and methane production. This study investigated the application of a current-carrying-coil-based magnetic field (CCC-MF) to AD of CM. The CCC-MF digesters showed higher soluble chemical oxygen demand and attained 16.59 % higher ammonium nitrogen reduction, contributing to a 37.50 % higher average methane yield than the control. Further, CCC-MF digesters showed higher enzyme activities (alkaline protease + 30 %, acetate kinase + 22 % and hydrazine dehydrogenase + 26 %) and increased microbial metabolic indices (dehydrogenase activity + 17 % and electron transport system activity + 10 %) than the control. Metagenomics analysis revealed that abundances of the bacterial genera Mesotoga, Aminobacterium, Xiashengella, unclassified Candidatus Cloacimonadota, Advenella, Pseudomonas, and Comamonas increased, whereas the acetoclastic methanogen Methanothrix decreased by 2.58 %, accompanied by 2.07- and 1.64-fold increases in hydrogenotrophic methanogens Methanospirillum and Methanobacterium, respectively, in CCC-MF digesters. The abundance of nitrogen dissimilation and assimilation genes NirK, NorB, NarB, NapA, nmo, and GLT1 were enhanced by 1.14, 1.04, 2.30, 1.32, 1.17, and 1.29-fold in CCC-MF digesters compared to the control. Moreover, metaproteomics revealed higher up-regulated differentially expressed proteins in NH₃ reduction-related amino acid metabolism pathways in CCC-MF digester compared to control. Additionally, reduced abundances of bacitracin, polymyxin, sulfonamide, and multidrug antibiotic resistance (MAR) gene types were observed in the CCC-MF digesters. The findings suggest that applying CCC-MF may be associated with higher methane production and ammonium reduction, potentially linked to a more favorable synergistic methanogenic system and nitrogen transformation pathways.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135326"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148389466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignin-polyphenol epoxy layer: a multi-functional protective coating cascade-constructed by ionic liquids.","authors":"Cheng Li, Yuting Shi, Wenzhe Xiao, Xiaoning Wang, Wanting Zhao, Wenjin Li, Shangru Zhai, Jian Sun","doi":"10.1016/j.biortech.2026.135216","DOIUrl":"10.1016/j.biortech.2026.135216","url":null,"abstract":"<p><p>Industrial lignin-derived polyphenols are promising platform chemicals, especially as bisphenol A substitutes for bio-resin production. Nevertheless, there is an urgent need to address the issues caused by traditional volatile and toxic epoxy curing agents. Herein, a novel lignin-polyphenol epoxy layer with multi-functional protective performances was successfully constructed through cascade reactions by ionic liquids (ILs). Specifically, lignin polyphenol obtained from demethylation of alkali lignin in ethanolamine acetate was cross-combined with salicylol (SA) and epichlorohydrin to synthesize lignin-based epoxy prepolymers by tetrabutylammonium bromide. And the epoxy layer was eventually obtained by solidification with dicarboxylic acid based PILs, [EOA]₂[Asp]. The resin layer has demonstrated excellent anti-corrosion, anti-ultraviolet and anti-bacterial properties. The iron sheet coated with resin layer (contact angle = 103.23°) exhibited corrosion resistance more than 2  months without any damage. A thin resin layer (0.5  mm) can achieve both excellent UV absorption properties (≥0.66 a.u.) and high UV blocking rate (96%). The positive Zeta surface potential of the resin layer is conducive to the adsorption of bacteria contributing additional antibacterial properties. Compared to lignin, lignin polyphenols can create more epoxy-binding sites and thus be cross-linked (ρ = 60.33 × 10^-3 mol/cm³) and cured with high density by more dicarboxylic acid based PILs. Meantime, the anions of the dicarboxylic acid based PILs contain multiple carboxyl groups, which can form additional dynamic physical crosslinking with the pre-polymer. This study explores new ways to utilize industrial lignin and develop ILs as green solvents for lignin-based processes.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135216"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148292732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaugmentation in treatment wetlands: Opportunities, challenges, and safety considerations.","authors":"Nianwen Chen, Huaqing Liu, Jizhen Li, Longlong Zhang, Dongle Cheng, Yanlong Wang, Guang Yang, Jian Zhang","doi":"10.1016/j.biortech.2026.135382","DOIUrl":"10.1016/j.biortech.2026.135382","url":null,"abstract":"<p><p>Bioaugmentation has been increasingly applied to enhance pollutant removal in treatment wetlands (TWs). However, its wider implementation is still constrained by limited understanding of long-term performance stability and ecological safety. Here, this review synthesizes the current applications of microbial inoculation strategies, highlighting emerging opportunities, key challenges, and critical safety considerations for implementation. The potential avenues toward sustainable bioaugmentation are summarized. These include alternative nitrogen removal processes, multi-pathway elimination of emerging contaminants (ECs), safe detoxification of heavy metals (HMs), and nutrient removal under extreme environmental conditions. Subsequently, key constraints limiting implementation are discussed, including the isolation of indigenous functional microorganisms and the reduced performance stability of inoculants under complex environmental conditions. To address these limitations, approaches such as Raman-activated cell sorting (RACS) and inoculant immobilization have been proposed. Finally, the ecological risks potentially associated with bioaugmentation were evaluated to ensure the safe implementation of microbial inoculation. These risks include disruption of native microbial communities, horizontal transfer of antibiotic resistance genes, and formation of toxic transformation products. Overall, this review provides insights into sustainable, site-specific bioaugmentation strategies in TWs and supports ecological engineering approaches for the remediation of diverse pollutants.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135382"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to \"Cost-effective wireless system for image-based microalgae biomass estimation with adaptive background correction\" [Bioresour. Technol. 459 (2026) 135189].","authors":"Hua Xiao, Qiaoyang Zhang, Haiyun Chen, Junjie Hao, Haodong Tang, Kai Wang, Xiao Wei Sun","doi":"10.1016/j.biortech.2026.135351","DOIUrl":"10.1016/j.biortech.2026.135351","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135351"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and multi-omics analyses reveal distinct effects of green light and increased silicate supply on cellular fucoxanthin content and fucoxanthin titer in Thalassiosira weissflogii.","authors":"Ruibin Sun, Dongxu Li, Ce Liu, Mengzhen Cheng, Xu Gao, Zhanru Shao","doi":"10.1016/j.biortech.2026.135365","DOIUrl":"10.1016/j.biortech.2026.135365","url":null,"abstract":"<p><p>Fucoxanthin (FX) is a high-value carotenoid in diatoms, yet how green light and increased silicate supply differentially affect cellular FX content and FX titer remains incompletely resolved. Here, Thalassiosira weissflogii was cultured under identical light intensity with four treatments: white light (WL), green light (GL), high silicate treatment corresponding to 2 × basal silicate concentration (HS), and their combination (GLHS). Physiological measurements were integrated with transcriptomic analysis, untargeted metabolomics, and targeted carotenoid quantification. GL induced a modest but significant increase in cellular FX content (11.71 ± 0.19 mg g^-1 dry cell weight [DCW]). This response was accompanied by transcriptomic and metabolomic changes related to carbon metabolism, isoprenoid precursor supply, and carotenoid biosynthesis. Under HS, cellular FX content, biomass, and FX titer showed no significant changes, despite broad upregulation of the fucoxanthin chlorophyll a/c-binding protein (FCP) gene family. GLHS achieved a significantly higher FX titer (1.92 ± 0.19 mg L^-1), accompanied by higher biomass but not a further increase in cellular FX content. In addition, heterologous expression and in vitro enzymatic assays of carotene isomerase 5 from T. weissflogii (TwCRTISO5) provided enzymatic support for its putative involvement in a terminal step of FX biosynthesis. Collectively, GL was associated with a modest increase in cellular FX content, whereas the higher FX titer under GLHS mainly reflected biomass gain. These findings provide a physiological and multi-omics basis for interpreting the effects of green light and increased silicate supply on cellular FX content and FX titer. They may inform diatom FX production strategies.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135365"},"PeriodicalIF":8.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surfactant-induced extremozyme production and aqueous separation for bioprocess intensification.","authors":"María S Álvarez, María A Longo, Francisco J Deive, Ana Rodríguez","doi":"10.1016/j.biortech.2026.136101","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136101","url":null,"abstract":"<p><p>Extremophiles constitute a valuable source of robust biocatalysts, although their industrial implementation is still constrained by limited production yields and costly downstream processing. In this work, a sustainable strategy combining surfactant-induced fermentation and deep eutectic solvent-based extraction was developed for the intensified production and recovery of an extracellular thermolipase from Bacillus thermoamylovorans CH6B. Triton X-102 was first evaluated as a lipase production inducer, leading to extracellular activities above 550 U L⁻¹ after 48 h at 55 °C. Biomass growth and enzyme production were successfully described by logistic and Luedeking-Piret models, confirming that lipase synthesis is predominantly growth-associated. Subsequently, Aqueous Biphasic Systems composed of Triton X-102 and choline chloride-based deep eutectic solvents were investigated at 45-65 °C. Among the solvents evaluated, choline chloride:ethylene glycol exhibited the best phase-forming ability together with the greatest thermal stabilization effect on the enzyme, increasing the Gibbs free energy of thermal deactivation to 172.1 kJ mol⁻¹. Under optimized tie-line conditions at 55 °C, the selected system recovered approximately 95 % of the extremolipase into the deep eutectic solvent-rich phase while preserving enzyme activity. Finally, a preliminary process simulation, intended as a proof-of-concept assessment, indicated the potential technical viability of integrating fermentation and extraction at a larger scale, estimating a production of 7.75 t of enzyme-rich extract from 131 batches/year; a comprehensive evaluation of industrial and economic feasibility would require further study. These results highlight surfactant-assisted fermentation coupled with deep eutectic solvent-based extraction as a promising strategy for sustainable extremozyme downstream processing, warranting further evaluation at larger scale.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136101"},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding photosynthesis through biosolar oxic N₂ fixation: toward a sustainable BioNitrogen Economy.","authors":"Taufiq Nawaz, Liping Gu, James Young, Shah Fahad, Nick Klein, Rajesh Sani, Ruanbao Zhou","doi":"10.1016/j.biortech.2026.136096","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136096","url":null,"abstract":"<p><p>Atmospheric dinitrogen (N₂) is far more abundant than carbon dioxide (CO₂), yet photosynthesis has been studied and harnessed predominantly for photosynthetic carbon fixation (PCF), underpinning the global biocarbon economy. In contrast, biological N₂ fixation-the natural gateway for atmospheric nitrogen into the biosphere-remains comparatively underexploited. Modern agriculture and industry consequently depend heavily on the fossil-fuel-intensive Haber-Bosch process, with substantial energy and environmental costs. Diazotrophic cyanobacteria offer a fundamentally different paradigm by coupling oxygenic photosynthesis to nitrogenase-mediated N₂ fixation. We term this process photosynthetic nitrogen fixation (PNF), which uses solar energy to convert water and atmospheric N₂ into bioavailable nitrogen. Beyond its ecological significance, PNF expands the functional scope of photosynthesis beyond carbon fixation and provides a biological foundation for sustainable nitrogen production, biofertilization, and N₂-based biosolar manufacturing. Recent advances in cyanobacterial physiology, ecology, evolution, synthetic biology, and AI-enabled multi-omics are accelerating efforts to harness this potential. This review examines the diversity of diazotrophic cyanobacteria, their strategies for protecting oxygen-sensitive nitrogenase, and emerging approaches for genetic engineering cyanobacteria as programmable platforms for nitrogen production and crop-associated nitrogen delivery. We further propose a roadmap toward a solar-powered BioNitrogen Economy encompassing bioprospecting, synthetic biology, genomic engineering, crop integration, and scalable agricultural and industrial deployment. By expanding photosynthesis from carbon fixation to the solar-powered capture of both carbon and atmospheric nitrogen, PNF could help drive the transition from a fossil-fuel-dependent chemo-nitrogen economy toward a sustainable BioNitrogen Civilization.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136096"},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of a highly active condition-responsive promoter for constructing a C1 gas-fermenting platform based on acetogen, Eubacterium callanderi KIST612.","authors":"Byeongchan Kang, Ji-Yeon Kim, Soyoung Oh, Minji Kim, Duleepa Pathiraja, In-Geol Choi, In Seop Chang","doi":"10.1016/j.biortech.2026.136069","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136069","url":null,"abstract":"<p><p>Integrating differential RNA-seq profiling with de novo motif discovery in Eubacterium callanderi KIST612 identified distinct sequence features associated with substrate-responsive transcription. Specifically, MOTIF4 and MOTIF17 showed significant correlations with glucose- and C1 gas (CO)-responsive gene expression, respectively. Based on these motif profiles, a candidate CO-responsive promoter, CI04, was selected and experimentally validated in vivo using a β-glucuronidase (GUS) reporter assay. CI04 exhibited specific CO-responsive activity, exceeding 500 mU mg^-1 under CO conditions. Under CO conditions, CI04 activity was approximately 10-fold higher than under glucose conditions, 59-fold higher than that of the constitutive (CT) promoter, and approximately 2.5-fold higher under CO conditions than that of the H2 promoter previously used for overexpression in E. callanderi KIST612.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136069"},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of organic wastes by black soldier fly (Hermetia illucens) for fertilizer and chitosan production: Process performance and resource recovery.","authors":"M D Madhushan, Nimesha Nuwan Chanuka, Nadeeka P Darshani, Kosala A Sirisena, Shoji Ikeda, Takahiro Watari, Choolaka Hewawasam","doi":"10.1016/j.biortech.2026.136095","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136095","url":null,"abstract":"<p><p>Organic waste management in tropical developing regions is constrained by the rapid accumulation of biodegradable residues and limited resource-recovery options. In this study, the bioconversion of food waste (FW), market waste (MW), kitchen waste (KW), and aquatic plant waste (AW) by black soldier fly (Hermetia illucens) larvae was investigated under controlled conditions in Sri Lanka. Larval performance, waste reduction, frass quality, plant response, and pupal-exuviae chitosan recovery were evaluated. FW produced the highest final larval weight of 264 ± 3 mg, followed by MW at 235 ± 1 mg. FW achieved an observed waste reduction of 83.7 %, which decreased to 80.1 ± 1.2 % after correction for natural substrate mass loss. In contrast, AW supported only limited larval growth (64 ± 1 mg) and resulted in 44.3 ± 2.1 % mortality, consistent with its low crude protein (2.35 ± 1.3 %), high crude fiber (68.2 ± 0.2 %), and high C: N ratio (30.3 ± 0.4). MW-derived frass contained 3.4 % N, 1.8 % P₂O₅, and 2.8 % K₂O, with a C: N ratio of 14.8, meeting or exceeding the selected SLS 1635:2019 requirements evaluated. In the greenhouse experiment, the 75 % MW-frass treatment produced 58 leaves plant^-1 compared with 45 leaves plant^-1 for commercial compost. Chitosan recovery from dry pupal exuviae reached 39.3 ± 0.1 %, with a mean degree of deacetylation of 98.1 ± 0.9 %. These findings demonstrate strong substrate-dependent differences in BSF bioconversion and support an integrated pathway for organic-waste reduction, frass production, and pupal-exuviae valorization.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136095"},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of recombinant human proteins in Komagataella phaffii using a sucrose-utilization-based selection marker without antibiotic resistance.","authors":"Mo Han, Mengli Li, Ruoyu Jia, Ming Miao, Tao Zhang","doi":"10.1016/j.biortech.2026.136081","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.136081","url":null,"abstract":"<p><p>Komagataella phaffii (K. phaffii) is one of the most widely used eukaryotic expression systems for recombinant protein production. However, its industrial application is constrained by reliance on traditional antibiotic-based selection and the inability to utilize sucrose. Based on the heterologous expression of the sucrose hydrolase SucF from Aspergillus flavus in K. phaffii, the strain acquired the ability to utilize sucrose. It was verified that this trait can serve as a dominant selection marker under sucrose-selective conditions, making SucF a selection marker for antibiotic-free strain construction. Under a selection pressure of 40 g/L sucrose, the gene integration efficiency reached 100 %, comparable to that of traditional antibiotic selection. Notably, this system enables the substitution of the original glycerol carbon source with sucrose for fermentation. To improve the selection efficiency of SucF, a triple mutant (T65A/R322Q/S323G) was identified through computer-aided protein engineering, exhibiting a 57 % increase in sucrose hydrolysis rate compared to the wild type. Structural analysis revealed that this mutation remodels the sucrose-binding pocket. Ultimately, the SucF mutant was used as a selection marker in combination with the histidine auxotrophic marker (HIS4) to achieve secretory expression of human lactoferrin (hLF) and human osteopontin (hOPN) in the GS115, and the resulting recombinant strain was free of antibiotic gene residues. This sucrose-based antibiotic-free platform in K. phaffii provides a promising strategy for green biomanufacturing and industrial production of recombinant proteins devoid of antibiotic resistance genes.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"136081"},"PeriodicalIF":8.2,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}