Bioresource Technology最新文献_第2页

Fostering nature-based solutions and circular approaches in biogas purification: validation of digestate centrate nitrified by intensified multi-stage constructed wetlands as electron acceptor in anoxic biodesulphurisation 在沼气净化中培育基于自然的解决方案和循环方法：强化多级人工湿地硝化的沼液浓缩液在缺氧生物脱硫中作为电子受体的验证

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-17 DOI: 10.1016/j.biortech.2025.133012

Rubén Hervás-Martínez, Núria Oliver, Tatiana Montoya, Feliu Sempere

{"title":"Fostering nature-based solutions and circular approaches in biogas purification: validation of digestate centrate nitrified by intensified multi-stage constructed wetlands as electron acceptor in anoxic biodesulphurisation","authors":"Rubén Hervás-Martínez, Núria Oliver, Tatiana Montoya, Feliu Sempere","doi":"10.1016/j.biortech.2025.133012","DOIUrl":"10.1016/j.biortech.2025.133012","url":null,"abstract":"<div><div>Anoxic biodesulphurisation using a biogenic nitrified source as an electron acceptor is a potential alternative to aerobic H<sub>2</sub>S biotreatment. This work reports the successful combination of nitrified centrate obtained from innovative constructed wetlands (CWs) with anoxic suspended biomass bioreactors (SBBs) treating real biogas. The quality of the liquid medium on the biodesulphurisation process was evaluated. The SBBs (of 48 L and 130 L, respectively) shifted seamlessly from using nitrified centrate from activated sludge to nitrified centrate from CWs, but its low orthophosphate content did limit H<sub>2</sub>S removal. To overcome this limitation, raw centrate was supplied. When nitrate + nitrite and orthophosphate concentrations were above 10 mgN L<sup>−1</sup> and 10 mgP L<sup>−1</sup>, 92 % of the H<sub>2</sub>S was removed in SBB1 (H<sub>2</sub>S elimination capacity (EC) of 3.0 gH<sub>2</sub>S Nm<sup>−3</sup>h<sup>−1</sup>), and 95 % in SBB2 (H<sub>2</sub>S-EC of 13.1 gH<sub>2</sub>S Nm<sup>−3</sup>h<sup>−1</sup>) at a gas residence time of 4.0 min and 3.1 min respectively.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 133012"},"PeriodicalIF":9.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Riboflavin-mediated reactivation of aged nano zero-valent iron by Shewanella oneidensis MR-1 for sustained Cr(VI) detoxification 核黄素介导的老龄纳米零价铁由希瓦氏菌MR-1持续解毒Cr（VI）

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-17 DOI: 10.1016/j.biortech.2025.133006

Chengyu Yang , Jie Wei , Yixuan Li, Yuanhao Peng, Xuan Wu, Xianrui Zhang, Yingxin Ding, Shumi Lu, Chongling Feng, Chao Huang

{"title":"Riboflavin-mediated reactivation of aged nano zero-valent iron by Shewanella oneidensis MR-1 for sustained Cr(VI) detoxification","authors":"Chengyu Yang , Jie Wei , Yixuan Li, Yuanhao Peng, Xuan Wu, Xianrui Zhang, Yingxin Ding, Shumi Lu, Chongling Feng, Chao Huang","doi":"10.1016/j.biortech.2025.133006","DOIUrl":"10.1016/j.biortech.2025.133006","url":null,"abstract":"<div><div>Aging-induced surface passivation of nano zero-valent iron (nZVI) significantly diminishes its reactivity for Cr(VI) reduction, thereby impeding its practical application in environmental remediation. Herein, we propose a novel system integrating aged nZVI (AnZVI), <em>Shewanella oneidensis</em> MR-1, and the endogenous electron shuttle riboflavin (RF) to overcome the passivation limitations and enhance Cr(VI) removal. Remarkably, the AnZVI/MR-1/RF system achieved complete removal of 20 mg L<sup>−</sup><sup>1</sup> Cr(VI) within 3 h, outperforming AnZVI/MR-1 and AnZVI alone by 23.8 % and 36.6 %, respectively. Mechanistic investigations revealed that RF mediated direct interfacial contact between AnZVI and MR-1, promoting microbial reduction of the AnZVI passivation layer. Electrochemical analyses confirmed RF’s critical role as an electron mediator in accelerating charge transfer between AnZVI and MR-1. Three-dimensional fluorescence mapping (3D-EEM) analysis revealed microbial extracellular polymeric substances (EPS) contributions to chromium immobilization processes. The immobilization of chromium is primarily attributed to the reduction of Cr(VI) to insoluble Cr(III) species, in conjunction with adsorption and surface complexation mechanisms. TEM validated a significant reduction in the thickness of the iron passivation layer, resulting in the exposure of the underlying Fe⁰ core and a resultant enhancement in reductive capacity. This work provides a robust strategy to overcome AnZVI’s limitations by coupling microbial activity with electron shuttling, offering new insights into sustainable remediation via biotic-abiotic synergies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 133006"},"PeriodicalIF":9.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nearly 100% selective hydrolysis of pyrolytic sugar over sustainable carbon catalysts enables highly efficient production of bioethanol 在可持续碳催化剂上几乎100%选择性水解热解糖，使生物乙醇的高效生产成为可能

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-17 DOI: 10.1016/j.biortech.2025.133008

Hong-li Ma , Ming-fu Li , Ying-chuan Zhang , Qing-hua Huang , Li-qun Jiang , Zhen Fang

{"title":"Nearly 100% selective hydrolysis of pyrolytic sugar over sustainable carbon catalysts enables highly efficient production of bioethanol","authors":"Hong-li Ma , Ming-fu Li , Ying-chuan Zhang , Qing-hua Huang , Li-qun Jiang , Zhen Fang","doi":"10.1016/j.biortech.2025.133008","DOIUrl":"10.1016/j.biortech.2025.133008","url":null,"abstract":"<div><div>The hydrolysis of levoglucosan (LG)–the major component of biomass pyrolysis oil–is the crucial step to bridge pyrolysis and fermentation. This study reports a series of sulfonated carbon catalysts (SCCs) as sustainable Brønsted acids for LG hydrolysis, which exhibit an excellent glucose selectivity over other hydration products (<em>e.g.,</em> 5- hydroxymethylfurfural and light oxygenates). Notably, SCCs maintain a LG conversion rate of 95 % and a glucose yield of 90 % afterfour cycles of batch operations. Time-of-flight mass spectrometry and theoretical calculations further clarify the hydrolysis mechanism, as water-derived protons are activated by sulfonate groups of SCCs and hydrolyze LG through C<img>O bond cleavage and hydration. Moreover, SCC-catalyzed hydrolysate is used in fermentation to produce bioethanol, enabling the maximal yield of ∼ 99 %, which is comparable to pure glucose commodity and much efficient than that from the aqueous acid hydrolysis. This study provides a sustainable alternative to mitigate the demand on heterogeneous catalysts for pyrolysis oil upgrading towards net-zero production of biofuels and biochemicals.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 133008"},"PeriodicalIF":9.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behavior and mechanism of extracting manganese from braunite by pyrolysis reduction-acid leaching of sawdust 木屑热解还原-酸浸法提取锰的行为及机理

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-17 DOI: 10.1016/j.biortech.2025.133007

Jun Yang , Yali Feng , Haoran Li

{"title":"Behavior and mechanism of extracting manganese from braunite by pyrolysis reduction-acid leaching of sawdust","authors":"Jun Yang , Yali Feng , Haoran Li","doi":"10.1016/j.biortech.2025.133007","DOIUrl":"10.1016/j.biortech.2025.133007","url":null,"abstract":"<div><div>In this study, a novel method for extracting manganese from braunite through sawdust pyrolysis reduction roasting followed by acid leaching was investigated. The thermodynamic properties, mass loss and the composition of the released gases were analyzed using thermodynamic simulation software and thermogravimetric mass spectrometry (TG-MS). The reaction mechanism and kinetic equation for braunite reduction via sawdust pyrolysis were determined using the Kissinger method and Coats-Redfern model. Optimal process parameters were identified through parametric experiments and phase analysis. Results showed that reducing gases (CH<sub>4,</sub> CO, C<sub>2</sub>H<sub>4</sub>, H<sub>2</sub>) released during sawdust pyrolysis reacted with braunite, with the highest reaction rate at approximately 320°C. The kinetic equation is expressed as <span><math><mrow><mfrac><mrow><mi>d</mi><mi>α</mi></mrow><mrow><mi>dT</mi></mrow></mfrac><mo>=</mo><mfrac><mrow><mn>3.09</mn><mo>×</mo><msup><mrow><mn>10</mn></mrow><mn>14</mn></msup></mrow><mi>β</mi></mfrac><mi>e</mi><mi>x</mi><mi>p</mi><mfenced><mrow><mo>-</mo><mfrac><mrow><mn>179.24</mn></mrow><mrow><mi>RT</mi></mrow></mfrac></mrow></mfenced><mo>×</mo><mfenced><mrow><mfrac><mn>3</mn><mn>2</mn></mfrac></mrow></mfenced><mo>×</mo><msup><mrow><mo>(</mo><mn>1</mn><mo>-</mo><mi>α</mi><mo>)</mo></mrow><mfrac><mn>4</mn><mn>3</mn></mfrac></msup><mo>×</mo><msup><mrow><mfenced><mrow><msup><mrow><mfenced><mrow><mn>1</mn><mo>-</mo><mi>α</mi></mrow></mfenced></mrow><mfrac><mrow><mo>-</mo><mn>1</mn></mrow><mn>3</mn></mfrac></msup><mo>-</mo><mn>1</mn></mrow></mfenced></mrow><mrow><mo>-</mo><mn>1</mn></mrow></msup></mrow></math></span>, where the activation energy is 179.24 kJ/mol. Optimal conditions included a roasting temperature of 550 °C, sawdust dosage at 75 % of braunite mass, and a roasting time of 30 min, yielding a manganese extraction rate exceeding 92 %. These findings confirm the method’s feasibility and offer new insights into braunite processing.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 133007"},"PeriodicalIF":9.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of deep eutectic solvent on laccase activity and thermal stability 深共熔溶剂对漆酶活性和热稳定性的影响

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-16 DOI: 10.1016/j.biortech.2025.132992

Li Yang , Jia Chen , Kangshun Xie , Kaiwen He , Wenyang Sheng , Mi Zhou , Guangyang Jiang , Yongqiang Tian

{"title":"Effects of deep eutectic solvent on laccase activity and thermal stability","authors":"Li Yang , Jia Chen , Kangshun Xie , Kaiwen He , Wenyang Sheng , Mi Zhou , Guangyang Jiang , Yongqiang Tian","doi":"10.1016/j.biortech.2025.132992","DOIUrl":"10.1016/j.biortech.2025.132992","url":null,"abstract":"<div><div>Eighteen deep eutectic solvent (DES) systems were systematically evaluated to optimize laccase biocatalysis. Five hydrogen bond acceptors (HBAs) significantly enhanced both enzyme activity and thermal stability. The choline dihydrogen phosphate (ChDHP)-glycerol DES yielded the highest activity increase (198 %). Moreover, a 50 % betaine-sorbitol (BS) solution retained 92 % activity after 1 h at 70 °C—55-fold higher than buffer—addressing a key thermal stability challenge. Fluorescence spectroscopy (FS) and molecular docking revealed that DES solutions stabilized laccase’s native structure by forming hydrogen bonds with catalytic center histidine (His) and phenylalanine (Phe) residues. BS-laccase pretreatment increased glucose conversion by 30 % in distillers’ grains (DG) and 54 % in poplar wood (PW). These findings establish DES as a sustainable, low-cost co-solvent that boosts enzymatic efficiency, offering molecular insights into HBA-enzyme interactions to guide biocatalytic system design for circular bioeconomy.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132992"},"PeriodicalIF":9.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic biochar-cored halophilic aerobic granules: Novel strategy for rapid formation and structural stability enhancement 磁性生物炭核嗜盐好氧颗粒：快速形成和结构稳定性增强的新策略

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-16 DOI: 10.1016/j.biortech.2025.132994

Dong-Xu Zhou, You-Wei Cui, Ya-Nan Mi, Zhen-Ying Li, Rui-Chun Yang, Yuan Sui

{"title":"Magnetic biochar-cored halophilic aerobic granules: Novel strategy for rapid formation and structural stability enhancement","authors":"Dong-Xu Zhou, You-Wei Cui, Ya-Nan Mi, Zhen-Ying Li, Rui-Chun Yang, Yuan Sui","doi":"10.1016/j.biortech.2025.132994","DOIUrl":"10.1016/j.biortech.2025.132994","url":null,"abstract":"<div><div>Halophilic aerobic granular sludge (HAGS) effectively treats hypersaline wastewater, offering advantages of structural compactness, microbial diversity, long functional bacteria retention, and high shock tolerance. However, long start-up times and granule disintegration limit its application. This study evaluated biochar and magnetic biochar (MBC) as carriers to accelerate formation and enhance stability. MBC reduced start-up time by providing microbial attachment sites and resolved operational instability. MBC-cored HAGS exhibited higher microbial metabolic activity, increased extracellular polymeric substance secretion (critical for stability), and enhanced microbial diversity during stable operation. Crucially, MBC’s Fe<sub>3</sub>O<sub>4</sub> loading inhibited filamentous fungal overgrowth, reducing abundance from 2 × 10<sup>10</sup> (without material) to 3 × 10<sup>8</sup> copies/g dry sludge. Thus, MBC proves to be a key optimization strategy for HAGS, enabling reliable application in hypersaline wastewater treatment by accelerating granule formation and ensuring long-term stability through Fe<sub>3</sub>O<sub>4</sub>-mediated inhibition of fungal overgrowth.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132994"},"PeriodicalIF":9.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into pretreatment improving the cooperation of adhesive and planktonic microbes in anaerobic digestion. 预处理改善厌氧消化中粘附微生物和浮游微生物的合作的新见解。

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-15 DOI: 10.1016/j.biortech.2025.132993

Ligong Wang, Jinxiao Xu, Cheng Li, Xuejun Hu, Chao Song, Guangqing Liu, Chang Chen

{"title":"Novel insights into pretreatment improving the cooperation of adhesive and planktonic microbes in anaerobic digestion.","authors":"Ligong Wang, Jinxiao Xu, Cheng Li, Xuejun Hu, Chao Song, Guangqing Liu, Chang Chen","doi":"10.1016/j.biortech.2025.132993","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132993","url":null,"abstract":"<p><p>Interactions between adhesive and planktonic microbes critically regulate anaerobic digestion (AD) of lignocellulosic biomass, yet how pretreatment alters these microbial dynamics and consequently influence methane output remain unclear. This study employed HCl and NaOH as representative chemical pretreatments to investigate their effects on the dynamics of adhesive versus planktonic microbes during corn stalk (CS) digestion. Results showed 2 % NaOH-pretreated CS reached the highest methane yield (380.9 mL/g VS), 32.7 % increase than HCl pretreatment. Following NaOH pretreatment, the abundance of adhesive Proteinophilum sp. S20 increased by 61.7 %-280.4 % than HCl pretreatment. Moreover, the planktonic populations of acidogenic Dysgononadaceae bacteria zrk40 and Fermentimonas caenicola was also significantly increased. Additionally, NaOH pretreatment notably boosted the adhesive abundance of Methanocollius chikugoensis by 62.0 %-306.0 % relative to HCl pretreatment and favored hydrogenotrophic metagenesis process. These findings deepen theoretical understanding of microbial interactions and offer new insights into developing pretreatments enhancing microbial cooperation and methane yield in AD.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132993"},"PeriodicalIF":9.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Cycle steam explosion combined with subcritical water Hydrolysis: An integrated process for maximizing sugar yields from rice husk 多循环蒸汽爆炸与亚临界水水解相结合：一种最大限度地提高稻壳糖产量的综合工艺

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-15 DOI: 10.1016/j.biortech.2025.132989

Crisleine P. Draszewski, Emanuel P. Skolaude, Matheus H. Brincker, Sócrates C. Carneiro, Adriano Cancelier, Fernanda de Castilhos, Ederson R. Abaide

{"title":"Multi-Cycle steam explosion combined with subcritical water Hydrolysis: An integrated process for maximizing sugar yields from rice husk","authors":"Crisleine P. Draszewski, Emanuel P. Skolaude, Matheus H. Brincker, Sócrates C. Carneiro, Adriano Cancelier, Fernanda de Castilhos, Ederson R. Abaide","doi":"10.1016/j.biortech.2025.132989","DOIUrl":"10.1016/j.biortech.2025.132989","url":null,"abstract":"<div><div>This work investigated the valorization of rice husk (RH) through an integrated steam explosion (SE) and subcritical water hydrolysis (SWH) process, aiming to maximize the production of fermentable sugars while minimizing the formation of undesirable inhibitory molecules. The biomass was subjected to successive explosion cycles (1–3 cycles, 180 °C, 5 min each) followed by SWH (230 °C or 260 °C, S/F-80), with kinetic monitoring of the release of sugars, organic acids, and inhibitors. The results demonstrated that three cycles of SE combined with SWH at 230 °C represented the optimal condition, reaching 40.68 g of fermentable sugars/100 g RH – a 12x increase compared to the untreated biomass. This condition also drastically reduced the formation of inhibitors (HMF and furfural <0.6 g/100 g RH) and organic acids (Y<sub>OA</sub> = 5.83 g/100 g RH), with maximum sugar production occurring between 6–10 min of hydrolysis (5.4 g FS/100 g min). In contrast, SWH at 260 °C led to higher thermal degradation, especially after multiple explosions, reducing yields. The optimal hydrolysis time was established at 20 min, after which sugar production rates approached zero. Sequential pretreatment with 3 cycles of SE followed by SWH at 230 °C for 20 min is the most efficient strategy to convert rice husk into fermentable sugars, offering operational and environmental advantages compared to conventional methods. These findings open perspectives for application in biorefineries, highlighting the potential of RH as a sustainable feedstock.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132989"},"PeriodicalIF":9.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorisation of food waste through self-fermentation and photosynthetic bacterial protein production: efficiency, microbial dynamics and safety assessment 通过自发酵和光合细菌蛋白质生产食物垃圾的价值：效率，微生物动力学和安全性评估

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-15 DOI: 10.1016/j.biortech.2025.132982

Wei Zhao , Jizheng Zhang , Pengfei Hou , Guangming Zhang , Zeqing Long

{"title":"Valorisation of food waste through self-fermentation and photosynthetic bacterial protein production: efficiency, microbial dynamics and safety assessment","authors":"Wei Zhao , Jizheng Zhang , Pengfei Hou , Guangming Zhang , Zeqing Long","doi":"10.1016/j.biortech.2025.132982","DOIUrl":"10.1016/j.biortech.2025.132982","url":null,"abstract":"<div><div>Global food waste has become an important environmental challenge. In this study, we established a hierarchical valorisation system through ‘acidogenic fermentation–residue saccharification–photosynthetic bacteria (PSB) protein production’. Acidogenic self-fermentation for 72 h yielded liquid chemical oxygen demand (COD) and lactic acid levels of 56443.33 and 11634.64 mg/L, respectively. Solid-phase residues from the fermentation broth underwent enzymatic saccharification at 60 °C, yielding 68.7 % carbohydrate conversion and 16278 mg/L reducing sugar. The liquid phase was treated with PSB, which resulted in 95 %, 89.7 % and 66.6 % removal of lactic acid, COD and ammonium-nitrogen (NH<sub>4</sub><sup>+</sup>-N), respectively, and PSB biomass and protein of 1356.5 and 415.1 mg/g. The relative abundance of <em>Rhodopseudomonas</em> was 59.04 %, with a metabolic shift from carbohydrate utilization to amino acid synthesis. The enhanced expression of the genes <em>Rubisco</em> and <em>GAPDH</em> strengthened energy metabolism and promoted PSB growth. Safety assessments identified potential allergens in the PSB protein; however, in vitro digestibility reached 77.28 %. Heavy metal content was 85 %–97 % below the food safety limits, thus confirming the safety of the resulting protein.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132982"},"PeriodicalIF":9.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ion exchange and bioregeneration by partial nitritation/anammox for mainstream municipal wastewater treatment 部分硝化/厌氧氨氧化在主流城市污水处理中的离子交换和生物再生

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-07-15 DOI: 10.1016/j.biortech.2025.132990

Sheyla Chero-Osorio , Lanica Steele , Valerie Carson , Ananda S. Bhattacharjee , Meng Wang , John Kuhn , Sarina J. Ergas

{"title":"Ion exchange and bioregeneration by partial nitritation/anammox for mainstream municipal wastewater treatment","authors":"Sheyla Chero-Osorio , Lanica Steele , Valerie Carson , Ananda S. Bhattacharjee , Meng Wang , John Kuhn , Sarina J. Ergas","doi":"10.1016/j.biortech.2025.132990","DOIUrl":"10.1016/j.biortech.2025.132990","url":null,"abstract":"<div><div>Conventional biological nitrogen removal (BNR) processes for mainstream municipal wastewater (MMW) treatment have high energy and chemical costs. Partial nitritation/anammox (PN/A) has the potential to reduce the carbon footprint of BNR; however, its implementation for MMW treatment has been limited by the low ammonium and high organic matter concentrations in MMW, which prevent suppression nitrite oxidizing bacteria (NOB) and heterotrophic denitrifiers. In this study, after organic carbon diversion, ammonium was separated from MMW in a novel bench-scale sequencing batch biofilm reactor (SBBR) containing chabazite, a natural zeolite mineral with a high ammonium ion exchange (IX) capacity. After breakthrough, chabazite was bioregenerated by PN/A biofilms. Recirculation was applied from the bottom to the top of the column to create an aerobic zone (top) for ammonia-oxidizing microorganisms (AOM) and an anoxic zone (bottom) for anammox bacteria. Rapid IX-PN/A SBBR startup was observed after inoculation with PN/A enrichments. The time required for bioregeneration decreased with increasing recirculation rate, with high total inorganic nitrogen (TIN) removal efficiency (81 %) and ammonium removal rate (0.11 g N/L/day) achieved at recirculation velocity of 1.43 m/h. The core microbiome of the IX-PN/A SBBR contained a high abundance of bacteria of the phylum <em>Pseudomonadota</em> (15.27–20.62 %), <em>Patescibacteria</em> (12.38–20.05 %), <em>Chloroflexota</em> (9.36–14.23 %)<em>,</em> and <em>Planctomycetota</em> (7.55–12.82 %), while quantitative PCR showed the highest ammonia monooxygenase (<em>amoA</em>, 2.0 × 10<sup>2</sup>) and anammox copy numbers (<em>amx</em>, 1.0 × 10<sup>4</sup>) in the top layers. The single-stage IX-PN/A SBBR achieved stable BNR for >two years without chemical inputs, media replacement or brine waste production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 132990"},"PeriodicalIF":9.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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