Bioresource Technology最新文献

{"title":"Corrigendum to \"Anaerobic treatment of nitrogenous industrial organic wastewater by carbon-neutral processes integrated with anaerobic digestion and partial nitritation/anammox: Critical review of current advances and future directions\" [Bioresour. Technol. 415 (2025) 131648].","authors":"Zixuan Deng, Chengde Sun, Guangyi Ma, Xinzheng Zhang, Hongbo Guo, Tao Zhang, Yalei Zhang, Yong Hu, Dapeng Li, Yu-You Li, Zhe Kong","doi":"10.1016/j.biortech.2026.135148","DOIUrl":"10.1016/j.biortech.2026.135148","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135148"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148269886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-quality-dependent pigment remodeling and ¹³C incorporation dynamics in Haematococcus pluvialis revealed by confocal Raman microscopy and Raman-SIP.","authors":"Hanxu Xu, Shuai Zhang, Mostafa Gouda, Jiayue Lei, Yulun Dong, Wei Luo, Yong He, Xiaoli Li, Hailiang Zhang","doi":"10.1016/j.biortech.2026.135183","DOIUrl":"10.1016/j.biortech.2026.135183","url":null,"abstract":"<p><p>Light quality critically regulates pigment remodeling and carbon allocation in Haematococcus pluvialis, yet stage-resolved, single-cell quantification of these processes remains limited. Bulk pigment assays, confocal Raman spectroscopy, Raman chemical imaging, and ¹³C-based Raman stable isotope probing (Raman-SIP) were integrated to characterize pigment dynamics and de novo carbon incorporation across cultivation time and division stages under white, red, and blue light. A spectral correction was established to reduce carotenoid interference in the chlorophyll-related region, enabling clearer separation of pigment pools and improved agreement with bulk measurements. Blue light selectively promoted carotenoid accumulation, increased single-cell heterogeneity, and induced stage-dependent spatial reorganization, whereas red light preferentially enhanced chlorophyll-related signals. Raman-SIP revealed progressive ¹³C incorporation into pigment-associated molecular structures under all light treatments. Blue light produced the highest labeling fraction for the carotenoid-associated 1156/1137 cm^-1 pair on Day 7 (52 %), whereas red light showed the highest corrected labeling fraction for the chlorophyll-related 1521/1490 cm^-1 pair (51 %). Raman imaging further revealed pronounced intracellular heterogeneity and hotspot-like carotenoid distributions under blue light, with transmission electron microscopy providing ultrastructural context. This integrated, stage-resolved single-cell framework directly links light-quality regulation to pigment remodeling and carbon allocation dynamics in H. pluvialis.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135183"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Linking microbial functional partitioning with methane productivity and stability in microbial electrolysis cell-anaerobic digestion hybrid systems\" [Bioresour. Technol. 453 (2026) 134629].","authors":"Xue-Ting Wang, Yanxue Li, Yuxin Pan, Xue Xing, Bo Wang, Defeng Xing, Jun Nan, Jonathan Tian En Lee, Eakalak Khan, Daniel C W Tsang, Nan-Qi Ren, Yong Sik Ok","doi":"10.1016/j.biortech.2026.135308","DOIUrl":"10.1016/j.biortech.2026.135308","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135308"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148417221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen supplementation improves glucose-based n-caproate production in Caproiciproducens galactitolivorans with reverse β-oxidation-associated redox remodeling.","authors":"Pranav Sasidharan Nair, Hyunjin Kim, Seongcheol Kang, Byoung Seung Jeon, Largus T Angenent, Byoung-In Sang","doi":"10.1016/j.biortech.2026.135089","DOIUrl":"10.1016/j.biortech.2026.135089","url":null,"abstract":"<p><p>Medium-chain carboxylic acids, such as n-caproate, are attractive sustainable platform chemicals. However, their microbial production is often limited by electron availability and low product selectivity. This study investigated the effect of hydrogen supplementation on glucose-based n-caproate production by Caproiciproducens galactitolivorans. Batch fermentation was performed under various hydrogen pressures (0 to 600 kPa). At 600 kPa hydrogen_,n-caproate reached 8.0 g L^-1, accompanied by a 66% increase in n-caproate selectivity and a tenfold increase in the intracellular redox-cofactor ratio, consistent with enhanced redox-cofactor turnover. Multi-omics analysis indicated metabolic remodeling under hydrogen-enriched conditions, including the increased abundance of key enzymes in the reverse β-oxidation pathway and redox-associated functions. In contrast, although fatty acid biosynthesis genes were transcriptionally induced, this transcriptional increase was not reflected at the protein level, suggesting a metabolic response more consistent with reverse β-oxidation-supported n-caproate synthesis than with fatty acid biosynthesis. These results support the use of hydrogen as a clean external reducing agent for improving n-caproate yield and redox efficiency in defined microbial systems. This study provides mechanistic insights into redox-driven metabolic control and selective n-caproate production in a defined microbial chain elongation system.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135089"},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148194481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering synergies between thermochemical and biochemical processes for municipal solid waste management and sustainable fuels supply.","authors":"Jinyang Lu, Ghulam Tariq, Suthajini Thiruketheeswaranathan, Shakil Ahmed Jiskani, Arun K Vuppaladadiyam, Wei-Shan Chen, Strik P B T B David, Ming Zhao","doi":"10.1016/j.biortech.2026.135799","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135799","url":null,"abstract":"<p><p>Municipal solid waste (MSW) valorization is constrained by the mismatch between heterogeneous feedstock properties and the operating requirements of individual conversion technologies. This review synthesizes integrated thermochemical and biochemical pathways and treats MSW valorization as a system design problem organized around carbon cascading, in which each carbon fraction is routed to the process able to convert it and performance is governed by interactions between processes rather than by individual process yields. Four hybrid routes are examined: gasification-syngas fermentation, pyrolysis-anaerobic digestion, hydrothermal processing-anaerobic digestion, and reverse configurations in which digestion precedes thermochemical conversion. Across these routes, intermediate quality rather than process yield governs feasibility. Tar (1-150 g/Nm³), ammonia (up to 14,000 ppmv), and sulfur species must be reduced by two to three orders of magnitude before downstream bioconversion, relocating the critical engineering problem from the reactor to the interface. Environmental and economic evidence indicates that integration benefits are real but conditional. Coupling anaerobic digestion with pyrolysis approximately doubled the climate mitigation of standalone digestion, yet the advantage is not uniform across impact categories, and one configuration achieved the greatest climate benefit while increasing freshwater ecotoxicity 52-fold. Reported production costs for integrated routes ($0.31-1.95/L ethanol) exceed those of standalone biochemical conversion, though integrated systems convert carbon that cheaper routes cannot access, and scale and product value influence viability more than process configuration. Integration is therefore justified by feedstock coverage and interface management rather than by coupling processes as such, and full-chain demonstration on real MSW remains the principal research challenge.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135799"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotic and abiotic degradation of PHAs: mechanisms, environments, and potential applications of degradation products.","authors":"Ariel Vilchez, Mario Sepúlveda, Michael Seeger, Francisca Acevedo, Rodrigo Navia","doi":"10.1016/j.biortech.2026.135766","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135766","url":null,"abstract":"<p><p>This review seeks to compile Polyhydroxyalkanoates (PHAs) degradation studies published over the past 20 years. It highlights the effect of physical properties, such as crystallinity and molecular weight, on the decomposition rate of these molecules. Both biotic processes, mediated by bacteria, fungi, and enzymes, as well as abiotic processes, such as hydrolysis and thermal degradation, are analyzed. A repertoire of diverse microorganisms, including their metabolic pathways and enzymes for PHA breakdown, is presented. Furthermore, this review presents the decomposition of PHAs in various environments, such as soil and seawater, highlighting their potential as a sustainable alternative. Finally, the resulting degradation products are described, emphasizing their potential applications in medicine and industry. Although degradation of PHAs has been extensively studied through these years, several knowledge gaps remain undisclosed, including the degradation of diverse polyester monomers. PHAs comprise numerous monomer compositions with variable properties, which present opportunities for different applications but pose a challenge in their degradation. The reader of this review can extract useful information for both the production of PHAs and their potential applications.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135766"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computationally assisted semirational engineering of Nicotiana attenuata α-glucan phosphorylase enhances cellobiose-to-amylose cascade synthesis.","authors":"Wei Gao, Mengli Li, Ming Miao, Tao Zhang","doi":"10.1016/j.biortech.2026.135787","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135787","url":null,"abstract":"<p><p>Converting non-food cellulosic sugars into programmable amylose offers a route to higher-value carbohydrate materials. α-Glucan phosphorylase (αGP) controls glucose-1-phosphate (G-1-P)-dependent chain elongation in cellobiose-to-amylose cascades. Here, Nicotiana attenuata αGP (NicαGP) was engineered by integrating AlphaFold3-based structural modeling, ProteinMPNN, EVcouplings, structural priors, and DynaMut2 filtering. Of 916 candidate substitutions, 37 were selected for experimental validation, and combinatorial screening identified L226Y/V437L/F751H as the optimal triple mutant, with 2.10-fold higher relative activity than the same-batch wild-type (WT) control and clear positive epistasis. The mutant further shifted the optimal temperature from 40 to 45 °C, increased thermal transition midpoint from 56.2 to 58.8 °C, and enhanced catalytic efficiency toward both maltotetraose and G-1-P. In the Clostridium thermocellum cellobiose phosphorylase (CtCBP)-NicαGP cascade, L226Y/V437L/F751H reached 38.86 ± 2.06 % conversion at 72 h versus the previously reported WT value of 35.84 ± 1.04 % at 84 h, increasing apparent productivity by 26.5 %. Docking, CAVER, and molecular dynamics (MD) analyses suggested that these gains may be associated with optimization of structural regions surrounding the conserved catalytic core. This work provides an engineered αGP for amylose synthesis from cellobiose and supports peripheral regulatory engineering as a practical strategy for improving phosphorylase-based biocatalytic cascades.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135787"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-assisted continuous cultivation enables stable and high-productivity microalgal single-cell protein production.","authors":"Sheetal Kishor Parakh, Baiyu Liao, Yen Wah Tong","doi":"10.1016/j.biortech.2026.135798","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135798","url":null,"abstract":"<p><p>Growing demand for sustainable protein has increased interest in microalgal single-cell protein (SCP) production using waste-derived nutrients. However, batch cultivation is limited by progressive nitrogen depletion, which causes biomass protein content to decline below SCP specifications (>30% protein). This study evaluated membrane-assisted continuous cultivation for stable production of SCP-grade microalgal biomass from food waste digestate usingScenedesmus obliquus,Graesiella emersonii, andChlorella sorokinianain 3 L photobioreactors. In batch culture, protein-rich biomass was obtained only within a narrow 2-day window, with protein contents of 42-55%; after nitrogen depletion, protein content declined to 11-29% by day 8. In contrast, continuous cultivation sustained protein contents above 46% for up to 189 d, showing that continuous nitrogen supply can preserve SCP-grade protein content over extended operation. By decoupling solids retention time (SRT) from hydraulic retention time (HRT) using an immersed membrane, continuous operation was systematically optimized. An operating window of SRT 3 d and HRT 2-3 d achieved protein productivity up to 0.14 g/L/d while meeting the SCP protein-content criterion. These findings establish membrane-assisted continuous cultivation as a controllable strategy for stable, high-productivity production of SCP-grade microalgal biomass from food waste digestate.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135798"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective production of xylo-oligosaccharides and xylose for further application and co-production of fermentable sugars from corncob via controlled ammonium persulfate-assisted hydrothermal pretreatment.","authors":"Keyu Chen, Siqinwen Tang, Qinghan Zeng, Rui Zhang, Yuanyuan Zhu, Junhua Zhang, Junjun Zhu","doi":"10.1016/j.biortech.2026.135791","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135791","url":null,"abstract":"<p><p>Ammonium persulfate (APS)-assisted hydrothermal pretreatment (HP) is an emerging method for xylan depolymerization, yet its potential for integrated valorization of all biomass fractions remains underexplored. This study aimed to selectively produce xylo-oligosaccharides (XOS) and xylose from corncob via controlled APS-HP, evaluate XOS bioactivity, and ferment xylose hydrolysate to xylonic acid without detoxification, while assessing enzymatic digestibility of solid residues. Under 0.5 % APS at 150 °C for 90 min, an optimal XOS yield reached 40.90 % (based on xylan in corncob), whereas 1 % APS gave a maximal xylose yield of 56.34 %. The XOS hydrolysate at 50 mg/L improved Chinese cabbage (Brassica rapa L. var. pekinensis) seed germination, plant height, root elongation, and fresh biomass, revealing its agricultural bioactivity. Notably, the xylose hydrolysate without detoxification was converted to xylonic acid with a yield of 90.32 % within 24 h, and even a 2.15-fold concentrated hydrolysate achieved 90.36 %, demonstrating process robustness. Solid residues from XOS and xylose production exhibited excellent enzymatic digestibility, with glucose yields of 96.89 % and 99.03 %, respectively, at 20 FPU/g glucan. Preliminary techno-economic analysis and qualitative life cycle assessment are used to assess economic theoretical basis for practical industrial production. Collectively, this study presents a novel integrated biorefinery strategy enabling sequential production of bioactive XOS, fermentable xylose, and highly digestible solid residues from corncob via APS-HP, offering a promising route for comprehensive lignocellulosic carbohydrates utilization without requiring a separate detoxification step.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135791"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Explainable machine learning for simultaneous energy-effluent prediction and operational state classification in a modified sequencing batch reactor.","authors":"Bernard Lassimo Diawara, Zhihua Xu, Shuai Yang, Haiping Cong, Xin Wang, Mohamed Yateh, Long Youwei, Ricardo Domingos Manuel Ferro, Wangyang Chen, Yulin Tang","doi":"10.1016/j.biortech.2026.135790","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.135790","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"135790"},"PeriodicalIF":8.2,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}