Bioresource Technology最新文献_第2页

Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132460

Chaowei Zhou , Yingying Zhu , Peifang Ren , Jing Leng , Xuan Xia , Tianpeng Chen , Wenjun Sun , Pengpeng Yang , Huanqing Niu , Yong Chen , Hanjie Ying

{"title":"Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis","authors":"Chaowei Zhou , Yingying Zhu , Peifang Ren , Jing Leng , Xuan Xia , Tianpeng Chen , Wenjun Sun , Pengpeng Yang , Huanqing Niu , Yong Chen , Hanjie Ying","doi":"10.1016/j.biortech.2025.132460","DOIUrl":"10.1016/j.biortech.2025.132460","url":null,"abstract":"<div><div>The ever-growing demand for cost-effective and green biocatalytic transformations has prompted the rational design and development of robust biocatalytic tools. However, transformations are hindered by limited continuous process and enzymatic instability. Here, 10 Flo family related genes in <em>Komagataella phaffii</em> were systematically evaluated to assess their adhesive properties. For the first time, we identified the KpFlo11C domain of BSC1p as facilitating cell aggregation on carriers, thereby enhancing the biofilm immobilization process. Furthermore, an engineered <em>K. phaffii</em> strain, fixing β-galactosidase on the cell surface, was constructed by optimizing the signal peptide and gene dosage, for enhancing the efficiency of enzyme targeting and anchoring, as well as the proportion of cells displaying the enzyme. Finally, the KpFlo11C domain was overexpressed in this <em>K. phaffii</em> cell display system to construct the enzyme-cell@material biocatalyst, which exhibited robust continuous production of galacto-oligosaccharides (GOS) at a rate of 8.16 g/L/h in a 6-L fermenter. The development of this enzyme-cell@material biocatalyst, which offers a highly efficient, stable, low-cost, and simplified biocatalytic process, provides a direction for the application of other yeasts in large-scale industrial continuous production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132460"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of inorganic carbon on purple phototrophic bacteria polyhydroxyalkanoates production under high reductive stress environment.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132462

Juliana Roda Almeida, Maria Ascensão Miranda Reis, Joana Costa Fradinho

{"title":"Influence of inorganic carbon on purple phototrophic bacteria polyhydroxyalkanoates production under high reductive stress environment.","authors":"Juliana Roda Almeida, Maria Ascensão Miranda Reis, Joana Costa Fradinho","doi":"10.1016/j.biortech.2025.132462","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132462","url":null,"abstract":"<p><p>Fermented wastes rich in reduced compounds challenge anaerobic purple phototrophic bacteria (PPB) systems by causing redox imbalances. This study evaluated polyhydroxyalkanoates (PHA) production and CO<sub>2</sub> fixation by PPB mixed cultures as means to balance internal redox, under four inorganic carbon (IC) availability conditions in a sequencing batch reactor. Culture selection under permanent IC presence promoted higher microbial diversity, but, strongly dependent on IC to balance internal redox, even when more oxidized substrates were supplemented. Increasing IC limitation favoured Rhodopseudomonas palustris, revealing its redox balancing capability independently of IC fixation. PHA contents of 20-29% gPHA/gVSS were achieved across all IC availabilities, however, when IC was present growth was promoted over PHA production, indicating a preferable electron balance through CO<sub>2</sub> fixation. Nevertheless, cultures selected under feast-famine IC exhibited a preference for PHA accumulation under IC limitation, suggesting IC tuning as a potential growth-controlling strategy to boost phototrophic PHA production using reduced feedstocks.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132462"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Neurospora crassa for the production of xylitol and ethylene glycol from xylose

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-29 DOI: 10.1016/j.biortech.2025.132459

Caihong Ma , Jiacheng Liang , Hao Fang , Zi Wei Luo , Shaolin Chen , Chen Zhao

{"title":"Metabolic engineering of Neurospora crassa for the production of xylitol and ethylene glycol from xylose","authors":"Caihong Ma , Jiacheng Liang , Hao Fang , Zi Wei Luo , Shaolin Chen , Chen Zhao","doi":"10.1016/j.biortech.2025.132459","DOIUrl":"10.1016/j.biortech.2025.132459","url":null,"abstract":"<div><div>The use of pentose from lignocellulose is critical for economic production of biofuels and chemicals. In this study, a filamentous fungus, <em>Neurospora crassa</em>, was used to metabolize xylose and synthesize xylitol and ethylene glycol (EG). Firstly, xylulose kinase gene was knocked out to prevent excessive xylose usage for strain growth, resulting in 5.8 ± 0.3 g/L xylitol and 58.3 ± 2.6 mg/L EG. Through optimization, the xylitol accumulation in the hydrolysate containing xylose reached 62.0 ± 0.6 g/L. The expression of 6-phosphofructokinase increased EG production with a maximum titer of 102.1 ± 1.8 mg/L, proving that the strain synthesized EG through xylulose-1-phosphate pathway. Addition of furfural or hydrolysate, and oxygen-limited environment were conducive to the EG accumulation. Further optimized strain accumulated EG up to 2586.4 ± 198.9 mg/L. This study provided the evidence of an all-natural metabolic pathway for EG synthesis in <em>N. crassa</em> and also demonstrated a new chassis cell for synthesis of xylitol and EG from xylose-rich hydrolysate.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132459"},"PeriodicalIF":9.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient nepetalactone production in Saccharomyces cerevisiae via metabolic engineering and bioprocess optimization.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-28 DOI: 10.1016/j.biortech.2025.132440

Herong Wang, Mengying Shan, Qi Gao, Jia Wang, Ruixuan Zhang, Ying Wang, Mingdong Yao, Wenhai Xiao

{"title":"Efficient nepetalactone production in Saccharomyces cerevisiae via metabolic engineering and bioprocess optimization.","authors":"Herong Wang, Mengying Shan, Qi Gao, Jia Wang, Ruixuan Zhang, Ying Wang, Mingdong Yao, Wenhai Xiao","doi":"10.1016/j.biortech.2025.132440","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132440","url":null,"abstract":"<p><p>Nepetalactone, a natural insect repellent comparable to N,N-diethyl-meta-toluamide (DEET), is challenging to produce through plant extraction or chemical synthesis. This study achieved the de novo synthesis of nepetalactone in Saccharomyces cerevisiae without expensive precursors or inducers. Initially, the metabolic pathway for nepetalactone synthesis was successfully established in Saccharomyces cerevisiae. A metabolic pathway was established using strategies such as iridoid synthase (ISY) source screening, enzyme fusion, and cofactor regeneration to optimize nepetalactone production. Bioprocess optimization through chromosomal integration and two-phase fermentation prevented its conversion to dihydronepetalactone, resulting in a high-yield strain, NTE21, with a titer of 2.5 g/L. A record titer of 4.5 g/L was achieved in 5.0 L fed-batch fermentation via continuous batch feeding. This study documents the potential of microbial platforms for the sustainable, cost-effective, and scalable production of nepetalactone, paving the way for its commercial application as a natural insect repellent.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132440"},"PeriodicalIF":9.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concentration-dependent effects of polystyrene microplastics on methanogenic activity and microbial community shifts in sewer sediments.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-28 DOI: 10.1016/j.biortech.2025.132464

Lucheng Li, Ting Xiao, Zanji He, Qijin Chen

{"title":"Concentration-dependent effects of polystyrene microplastics on methanogenic activity and microbial community shifts in sewer sediments.","authors":"Lucheng Li, Ting Xiao, Zanji He, Qijin Chen","doi":"10.1016/j.biortech.2025.132464","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132464","url":null,"abstract":"<p><p>Microplastics (MPs) are emerging environmental contaminants that interfere with microbial processes, yet their effects on methane production in anaerobic systems remain insufficiently understood. This study investigates the impact of polystyrene microplastics (PS-MPs) on methanogenesis, microbial community structure, and metabolic pathways in simulated sewer sediment systems, with exposure concentrations of 5, 50, and 250 mg·L<sup>-1</sup>. The results revealed a concentration-dependent effect of PS-MPs on methanogenesis: a 222.2 % increase at 5 mg·L<sup>-1</sup>, and 72.2 % and 88.9 % increases at 50 mg·L<sup>-1</sup> and 250 mg·L<sup>-1</sup>, respectively, indicating a non-linear response. PS-MPs exposure enhanced coenzyme F420 (F420) activity, a key indicator of methanogenic activity, but also inhibited Methyl coenzyme M reductase (Mcr), disrupting critical methanogenic pathways. At lower concentrations, PS-MPs promoted the abundance of hydrogenotrophic methanogens, whereas higher concentrations suppressed overall methanogenic activity. Furthermore, PS-MPs had a dose-dependent effect on CH<sub>4</sub> oxidation, influencing the structure of methanotrophic communities. These findings establish a clear dose-response relationship between PS-MPs concentration and CH<sub>4</sub> dynamics in anaerobic systems, highlighting the complex role of microplastics in methanogenesis and microbial interactions. This research provides valuable insights into the environmental implications of microplastics in wastewater systems and their potential impacts on biogas production and CH<sub>4</sub> mitigation, aligning with the objectives of environmental bioengineering and sustainable waste management.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132464"},"PeriodicalIF":9.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of ammonia and volatile fatty acids inhibition in dry anaerobic digestion of chicken manure by biochar prepared at varying pyrolysis temperatures

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-28 DOI: 10.1016/j.biortech.2025.132465

Youqian Xiao , Hongnan Yang , Xinru Jiang , Wenguo Wang , Liangwei Deng

{"title":"Mitigation of ammonia and volatile fatty acids inhibition in dry anaerobic digestion of chicken manure by biochar prepared at varying pyrolysis temperatures","authors":"Youqian Xiao , Hongnan Yang , Xinru Jiang , Wenguo Wang , Liangwei Deng","doi":"10.1016/j.biortech.2025.132465","DOIUrl":"10.1016/j.biortech.2025.132465","url":null,"abstract":"<div><div>This study examined the impact of various types of biochar on dry anaerobic digestion of chicken manure under the combined stress of ammonia and volatile fatty acids (VFAs). Total ammonia nitrogen (TAN) concentration at 9069 mg/L and total VFAs (TVFA) concentration at 33646 mg-HAc/L decreased the biogas production potential of chicken manure by approximately 50 %. The introduction of biochar prepared at 800 °C (BC800) enhanced the maximum biogas production rate of the inhibited anaerobic digestion mixture by 121.3 % and reduced the anaerobic digestion period by 38.6 %. The superior electrical conductivity, high specific capacitance value, large pore volume, and large specific surface area of BC800 significantly improved its performance in facilitating dry anaerobic digestion. BC800 enriched <em>Bathyarchaeia</em> and <em>Methanosaeta</em>, fostering the breakdown of propionic acid and bolstering acetoclastic methanogenesis. This study provides valuable experience for dry anaerobic digestion of chicken manure in future applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132465"},"PeriodicalIF":9.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current advances in microalgae-based fucoxanthin production and downstream processes.

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-27 DOI: 10.1016/j.biortech.2025.132455

Fitri Santy Budiarso, Yoong Kit Leong, Jui-Jen Chang, Chun-Yen Chen, Jih-Heng Chen, Hong-Wei Yen, Jo-Shu Chang

{"title":"Current advances in microalgae-based fucoxanthin production and downstream processes.","authors":"Fitri Santy Budiarso, Yoong Kit Leong, Jui-Jen Chang, Chun-Yen Chen, Jih-Heng Chen, Hong-Wei Yen, Jo-Shu Chang","doi":"10.1016/j.biortech.2025.132455","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132455","url":null,"abstract":"<p><p>Fucoxanthin, a marine carotenoid primarily found in brown algae and microalgae, offers significant health benefits, including antioxidant, anti-obesity, and anti-cancer effects. While brown algae remain the dominant commercial source, microalgae such as Phaeodactylum tricornutum are emerging as promising candidates for large-scale, sustainable fucoxanthin production. This review explores advancements in fucoxanthin biosynthesis, focusing on cultivation methods, extraction techniques, and genetic engineering strategies. Different cultivation systems - including autotrophic, heterotrophic, and mixotrophic approaches - have been assessed for their biomass yield, cost-effectiveness, and scalability, together with a quantitative meta-analysis to highlight specific trends or correlations in fucoxanthin production. The efficiency and environmental impact of extraction methods, such as supercritical fluid extraction, ultrasound-assisted extraction, and microwave-assisted extraction, have also been evaluated. In addition, synthetic biology and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based genetic modifications show potential for enhancing fucoxanthin biosynthesis. However, challenges remain in terms of cost, scalability, and regulatory constraints. This review highlights the need for integrated biotechnological solutions to enhance commercial viability, combining metabolic engineering, efficient extraction techniques, and optimized cultivation strategies. As demand continues to grow in the nutraceutical, pharmaceutical, and cosmetic industries, ongoing advancements in microalgae-based fucoxanthin production will be critical for ensuring sustainable and cost-effective manufacturing.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132455"},"PeriodicalIF":9.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobility and bio-accessibility of available phosphorus in sewage sludge: Influencing mechanism of hydrothermal pretreatment and incineration

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-27 DOI: 10.1016/j.biortech.2025.132429

Minghao Jin, Huan Liu, Hongping Deng, Hong Yao

{"title":"Mobility and bio-accessibility of available phosphorus in sewage sludge: Influencing mechanism of hydrothermal pretreatment and incineration","authors":"Minghao Jin, Huan Liu, Hongping Deng, Hong Yao","doi":"10.1016/j.biortech.2025.132429","DOIUrl":"10.1016/j.biortech.2025.132429","url":null,"abstract":"<div><div>Accurate assessment and enhancement of phosphorus (P) availability are critical for land application of sewage sludge and its thermal-treated products. By simulating different functioning pathways of P in soil, a novel multivariable scheme was developed to evaluate P availability from the perspective of mobility and bio-accessibility, then was applied to investigate the effects of hydrothermal pretreatment (HT), carbonaceous skeleton-assisted HT (CSkel-HT), and incineration on this topic. Sludge contained predominantly slow-release and microbial-available P (>50.0 % of total P). HT and incineration reduced available P through filtrate discharge, organic-P decomposition, and Fe/Al-P volatilization. Surprisingly, CSkel-HT addition promoted soluble Ca/MgHPO<sub>4</sub> and thermal-stable Fe/AlPO<sub>4</sub> formation under acidic conditions, which not only retained the slow-release and microbial-available P in hydrochar and ash, but also increased the rapid-available and plant-available P contents by 25.0 % and 300.0 %. Our scheme provided more informative insights than traditional single-index methods, and revealed the enhancing mechanism of CSkel-HT on P availability.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132429"},"PeriodicalIF":9.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of tar formation during integrated process of biomass anaerobic digestion and gasification

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-27 DOI: 10.1016/j.biortech.2025.132448

Xiaopeng Jia , Zhenyan Liu , Jian Li , Beibei Yan , Zhi Wang , Guanyi Chen

{"title":"Characteristics of tar formation during integrated process of biomass anaerobic digestion and gasification","authors":"Xiaopeng Jia , Zhenyan Liu , Jian Li , Beibei Yan , Zhi Wang , Guanyi Chen","doi":"10.1016/j.biortech.2025.132448","DOIUrl":"10.1016/j.biortech.2025.132448","url":null,"abstract":"<div><div>The tar problem is a bottleneck issue of biomass gasification. The influence of anaerobic digestion on gasification tar formation is still unclear. In this work, the influence of the degree of anaerobic digestion on physicochemical properties of the digestate were investigated, and consequently on tar yield and distribution. The content of cellulose exhibits the most critical parameter affecting tar yield. Minimum tar yields (2.5 wt%, 1.9 wt% and 1.6 wt% for pyrolysis, CO<sub>2</sub> gasification and H<sub>2</sub>O gasification) was obtained in day 9 with a maximum lignin content (29.5 wt%) and a minimum cellulose content (24.8 wt%). Both gasification agent of CO<sub>2</sub> and H<sub>2</sub>O significantly reduce tar yields. The tar distribution was always dominated by benzofuran, 2,3-dihydro- and phenol derivatives regardless of the agent. This work provides in-depth insights and clarifies the tar formation characteristic of the integrated process, which helps to realize the efficient and green conversion of biomass.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132448"},"PeriodicalIF":9.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversing lignin inhibition on enzymatic hydrolysis through regulating supramolecular assembly

IF 9.7 1区环境科学与生态学

Bioresource Technology Pub Date : 2025-03-27 DOI: 10.1016/j.biortech.2025.132451

Zhanghao Luan , Xin Wang , Wei Zhang , Xiaoyu Shi , Ruoyan Li , Chenhuan Lai , Daihui Zhang , Mi Li , Qiang Yong

{"title":"Reversing lignin inhibition on enzymatic hydrolysis through regulating supramolecular assembly","authors":"Zhanghao Luan , Xin Wang , Wei Zhang , Xiaoyu Shi , Ruoyan Li , Chenhuan Lai , Daihui Zhang , Mi Li , Qiang Yong","doi":"10.1016/j.biortech.2025.132451","DOIUrl":"10.1016/j.biortech.2025.132451","url":null,"abstract":"<div><div>The non-productive binding of enzymes to lignin is a well-documented barrier to efficient enzymatic hydrolysis. While significant attention has been paid to the influence of lignin’s chemical structure on enzyme-lignin interactions, the role of its supramolecular assembly in this process has largely been overlooked. In this study, we regulated the supramolecular assembly of ethanol organosolv lignin (EOL) through solvent exchange dialysis and investigated its impact on the enzymatic hydrolysis of cellulose. Our findings reveal that tuning the supramolecular assembly of lignin significantly influenced its physicochemical properties, particularly particle size and hydrophobicity. Larger lignin particles, formed through self-assembly during dialysis, exhibited reduced hydrophobicity. Notably, the size of the lignin aggregates exercised very distinct effects on the performance of enzymatic hydrolysis. Certain large lignin assembled particles even reversed its inhibitory effects on the enzymatic hydrolysis of Avicel. EOL-M1 with the smallest particle size decreased 72 h glucose yields from 70.5 % to 62.9 %, whereas EOL-M100 with the largest particle size increased 72 h glucose yields to 82.3 %. Langmuir adsorption isotherms analysis, and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that lignin with larger particle sizes and lower hydrophobicity reduced the enzyme-binding capacity. Furthermore, this phenomenon was consistently validated with three different lignin samples obtained from organosolv and kraft pulping processes. This study demonstrated the unusual functions of lignin particles in the enzymatic saccharification process. It also provides new insights into the mechanism underlying the influences of lignin on enzyme-lignin interactions and bioprocessing at large.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"428 ","pages":"Article 132451"},"PeriodicalIF":9.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0