Impact of microbial entrapment on sulfate-reducing bacteria performance and stability with temperature disturbances.

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI:10.1016/j.biortech.2025.133110

Xinting Yin, Nicholas Gurieff, Adrian Oehmen

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引用次数: 0

Abstract

Sulfate-reducing bacteria (SRB) can treat Acid and Metalliferous Drainage (AMD); however, process stability is challenging. This study evaluated microbial entrapment technology as an alternative solution by entrapping SRB in a porous hydrogel matrix, creating a stable microenvironment, while allowing diffusion of nutrients and gases. Two sequencing batch reactors (SBRs) were operated over 210 days: one with entrapped SRB (ESRB) and the other with non-entrapped SRB. The ESRB system exhibited greater sulfate reduction efficiency and operational resilience. It maintained rates of 0.71 ± 0.06 and 0.86 ± 0.05 g SO₄^2-/L/day during two 25-day operational periods with temperature drops from 24 °C to 15 °C. The non-entrapped SRB system dropped to 0.00 ± 0.00 and 0.12 ± 0.03 g SO₄^2-/L/day, respectively. Microbial community analysis revealed an increased proportion of SRB in the ESRB system. Compression tests and OD₆₀₀ confirmed bead integrity and biomass retention. This study supports the applicability of ESRB for AMD treatment.

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温度扰动下微生物包埋对硫酸盐还原菌性能和稳定性的影响。

硫酸盐还原菌（SRB）可以处理酸性和含金属废水（AMD）；然而，过程稳定性是具有挑战性的。本研究评估了微生物包埋技术作为一种替代方案，通过将SRB包埋在多孔水凝胶基质中，创造稳定的微环境，同时允许营养物质和气体扩散。两个序批式反应器（sbr）运行了210 天，一个是包封SRB (ESRB)，另一个是未包封SRB。ESRB体系表现出更高的硫酸盐还原效率和操作弹性。在温度从24 °C降至15 °C的两个25天的运行周期中，它保持了0.71 ± 0.06和0.86 ± 0.05 g SO42-/L/d的速率。无截留SRB系统分别降至0.00 ± 0.00和0.12 ± 0.03 g SO42-/L/d。微生物群落分析显示SRB在ESRB系统中的比例增加。压缩测试和OD600证实了颗粒的完整性和生物量保留。本研究支持ESRB在AMD治疗中的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.