Deciphering rapid proliferation of filamentous bacteria in activated sludge community via cross-feeding of public goods mediated by diffusible signal factor

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-07-14 DOI:10.1016/j.biortech.2025.132980

Huan Liu , Jie Li , Shao-Yang Liu , Jin-Song Guo , You-Peng Chen , Peng Yan

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引用次数: 0

Abstract

The induced mechanisms underlying filamentous bulking by quorum sensing (QS) remain unclear. This study investigated how diffusible signal factors (DSF)-mediated QS facilitates filamentous bacterial proliferation via public goods cross-feeding. The results indicated that DSF concentrations increased by 4.32– and 5.03-fold in the aqueous and solid phases, respectively, correlating positively with the proliferation of the filamentous bacterium Thiothrix and upregulation of DSF-related genes (rpfF, rpfC, rpfG). Activation of the DSF-QS system stimulated threonine and serine biosynthesis and extracellular protease secretion, supporting the auxotrophic growth of Thiothrix. Correlation and phylogenetic analyses suggested that DSF-QS regulates the cross-feeding of these metabolites between Thiothrix, Acinetobacter bohemicus B and Ca. Competibacter B. Excessive proliferation of Thiothrix disrupted the physicochemical properties of sludge flocs and impaired aggregation, leading to sludge bulking. This study reveals that DSF-QS promotes filamentous bulking in activated sludge by regulating the cross-feeding of public goods.

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可扩散信号因子介导的公共物品交叉喂养对活性污泥群落丝状菌快速增殖的解读

群体感应（QS）诱导丝状体膨胀的机制尚不清楚。本研究探讨了DSF介导的QS如何通过公共物品交叉喂养促进丝状细菌的增殖。结果表明，DSF在水相和固相中的浓度分别增加了4.32倍和5.03倍，与丝状菌硫垂菌的增殖和DSF相关基因（rpfF、rpfC、rpfG）的上调呈正相关。DSF-QS系统的激活刺激了苏氨酸和丝氨酸的生物合成和细胞外蛋白酶的分泌，支持硫喉虫的营养不良生长。相关分析和系统发育分析表明，DSF-QS调控了硫thrix与波希米不动杆菌B和Ca. Competibacter B之间代谢物的互食。硫thrix的过度增殖破坏了污泥絮凝体的理化性质，损害了污泥的聚集性，导致污泥膨胀。本研究表明，DSF-QS通过调节公共物品的交叉投料促进活性污泥中的丝状膨胀。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.