Response of anaerobic digestion of cattle manure to continuous temperature transitions: Process performance, microbial succession, key enzymes, and metabolic pathways

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-07-10 DOI:10.1016/j.biortech.2025.132953

Chenyang Zhu , Heran Wang , Rufei Liu , Hairong Yuan , Xiujin Li

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Abstract

This study investigated the effect of continuous temperature transitions on the anaerobic digestion (AD) performance of cattle manure (CM) and microbial response mechanisms. Experiments were conducted in a completely-stirred tank reactor (CSTR) for over 390 days with a fine 3 °C gradient from 35 °C to 65 °C. The results showed that AD achieved optimal performance at R47 (47 °C). Microbial analysis indicated that 47 °C was the critical temperature for the transition from mesophilic to thermophilic communities. Metagenomic analysis revealed that, compared with R38 (38 °C) and R53 (53 °C), R47 enhanced the hydrolysis efficiency of refractory components by upregulating key enzyme expression in glycolysis and pyruvate metabolism. At R47, elevated expression of key enzymes in both acetate/butyrate metabolic pathways and methylotrophic methanogenesis pathway contributed to the highest methane yield. The optimal temperature determined for CM provides important parameters for practical AD operations.

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牛粪厌氧消化对持续温度变化的响应：过程性能、微生物演替、关键酶和代谢途径

研究了连续温度变化对牛粪厌氧消化（AD）性能的影响及其微生物响应机制。实验在完全搅拌槽式反应器（CSTR）中进行，实验时间超过390天，温度梯度从35°C到65°C。结果表明，AD在R47（47°C）温度下达到最佳性能。微生物分析表明，47°C是由嗜温菌群向嗜热菌群转变的临界温度。宏基因组分析显示，与R38（38°C）和R53（53°C）相比，R47通过上调糖酵解和丙酮酸代谢关键酶的表达，提高了难降解组分的水解效率。在R47时，乙酸/丁酸代谢途径和甲基营养化产甲烷途径中关键酶的表达均有所增加，导致甲烷产量最高。CM的最佳温度为AD的实际操作提供了重要的参数。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.