Characterization of a novel Dye-Decolorizing peroxidase from Brevibacillus agri and its application in detoxification of textile industry wastewater

Dye-containing wastewater from the textile industry poses severe ecological and health risks. Here, we identified and characterized a novel dye-decolorizing peroxidase (BaDyP) from Brevibacillus agri, notable for its broad substrate range and catalytic robustness. Heterologous expression in Escherichia coli yielded superior activity compared to Saccharomyces cerevisiae. Recombinant BaDyP efficiently decolorized seven structurally diverse dyes, achieving removal rates of up to 96.7 % (Reactive Black 5, D240E variant), 95.0 % (Congo Red), and 93.2 % (Methylene Blue). BaDyP also decolorized authentic industrial effluent with 78.1 % efficiency, which was further enhanced to 93.3 % by the D240E mutant, representing a 19.5 % improvement. Importantly, BaDyP treatment reduced wastewater toxicity, as demonstrated by microbial growth assays, and structural dye degradation was validated by FTIR. Collectively, these findings highlight BaDyP as a promising biocatalyst for sustainable remediation of dye-contaminated industrial effluents.