Biomass & Bioenergy最新文献

{"title":"Biomass gasification in an autothermal semi-industrial fluidized bed gasifier: Syngas characterization and energy balance","authors":"P.O. Badu,&nbsp;M. Debal,&nbsp;P. Girods,&nbsp;S. Aubert,&nbsp;Y. Rogaume","doi":"10.1016/j.biombioe.2025.107831","DOIUrl":"10.1016/j.biombioe.2025.107831","url":null,"abstract":"<div><div>Biomass gasification is well-established in industrial applications, but semi-industrial autothermal systems remain underexplored despite their role as a bridge between laboratory scale and full-scale deployment. Understanding syngas characteristics, energy recovery, and system efficiency at this scale is critical for optimizing industrial gasification. This study examines air gasification of woody biomass in a 200 kW autothermal bubbling fluidized bed gasifier operating at equivalence ratios (ER) 0.22–0.45 with recorded bed temperatures of 695–860 °C. Key performance indicators, including syngas composition, lower heating values (LHV), cold gas efficiency (CGE), carbon conversion efficiency (CCE), and energy distribution of the near-industrial system, were analyzed. Results show syngas primarily contained H₂ (6.4–11.3 %), CO (8.5–16.6 %), CH₄ (3.0–5.5 %), and CO₂ (15.6–19.4 %), with LHV of 3.9–7.1 MJ/Nm³. CGE reached 51.8–71.4 %, and CCE 78.6–98.5 %. Overall system efficiency (79–93 %) highlights the role of energy recovery from syngas cooling and gathered solid residues in improving large-scale gasification viability. This study provides novel insights into optimizing semi-industrial gasification, demonstrating the feasibility of integrated energy recovery for enhanced efficiency and sustainability.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107831"},"PeriodicalIF":5.8,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of sustainable biogenic marine waste-based composite for phosphate ions recovery and subsequent biogas production","authors":"Huda M. Alghamdi ,&nbsp;Mohamed El-Qelish ,&nbsp;Khalid Z. Elwakeel ,&nbsp;Faten M. Ali Zainy ,&nbsp;Zhen Yang ,&nbsp;Ahmed M. Elgarahy","doi":"10.1016/j.biombioe.2025.107819","DOIUrl":"10.1016/j.biombioe.2025.107819","url":null,"abstract":"<div><div>This study introduces an innovative approach, addressing dual environmental challenges of water treatment and sustainable bioenergy generation. We have synthesized a sustainable biogenic agal-bivalve shells-based composite (CAL-GRABC) for phosphate recovery from aqueous solutions and subsequent biogas production. The findings revealed that the sorption process of PO₄³⁻ onto CAL-GRABC was pH-dependent with 94.12% removal efficiency under optimized pH ∼4.1. Meanwhile, kinetic studies indicated that the adsorption process conformed closely to PSORE model, while isotherm data were well-correlated with the Langmuir assumption, demonstrating a maximum loading capacity of 333.33 mg g⁻¹. Furthermore, the PO₄³⁻ adsorption process was endothermic. Interestingly, the used sorbent was managed particularly for biogas production resulting in a measured yields of 267 mL-CH₄ gVS^-1, which is 2.2 times the control. To sum up, this research highlights the dual functionality of the developed material, as a promising candidate for wastewater remediation and renewable energy production.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107819"},"PeriodicalIF":5.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical looping reforming of bioethanol for hydrogen production: Modeling and design of the fuel reactor of a 350 MW unit","authors":"Margarita de las Obras Loscertales ,&nbsp;Alberto Abad ,&nbsp;Luis F. de Diego ,&nbsp;Arturo Cabello ,&nbsp;Juan Ruiz ,&nbsp;Francisco García Labiano","doi":"10.1016/j.biombioe.2025.107830","DOIUrl":"10.1016/j.biombioe.2025.107830","url":null,"abstract":"<div><div>Chemical Looping Reforming of bioethanol offers an efficient process for the production of high-quality syngas which may be easily integrated with CO₂ capture technologies for the H₂ production with negative CO₂ emissions. In this work, the fuel reactor of a 350 MW Chemical Looping Reforming unit, fed with raw ethanol obtained after the first distillation, has been modeled. A macroscopic model was developed by integrating the fluid dynamics of a high-velocity fluidized bed with the kinetics of catalytic ethanol conversion. The Ni-based oxygen carrier exhibited sufficient catalytic activity and oxygen transport capacity to fully convert ethanol, with CO and H₂ as the main gas products. Simulation work was done to evaluate the syngas production as a function of the operating conditions. Eventually, the basic parameters for the design of the fuel reactor are defined. Results from mass and enthalpy balances indicated that suitable conditions to maximize syngas yield (4.3 mol H₂ per mole of bioethanol after WGS reactor) included a solids circulation of 1000 kg/s and a fuel reactor temperature of 800 °C. To support a suitable fluid dynamic, the cross area was fixed at 0.05 m²/MW, which defined an inlet gas velocity of 5.6 m/s and supported the desired solids circulation rate. The fuel reactor was set at 13.6 m height to accommodate the size of four cyclones, and a pressure drop value of 24 kPa is proposed to facilitate a suitable dense bed height of 0.5 m.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107830"},"PeriodicalIF":5.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational engineering of Pseudomonas putida DSM 6125 for dedicated catabolic detoxification of furfural and 5-(hydroxymethyl)furfural","authors":"Santosh Kumar,&nbsp;Eric Agyeman-Duah,&nbsp;Victor C. Ujor","doi":"10.1016/j.biombioe.2025.107839","DOIUrl":"10.1016/j.biombioe.2025.107839","url":null,"abstract":"<div><div>The prospect of using lignocellulosic biomasses (LCBs) as cost effective feedstocks in bio-production of chemicals is hampered by the inhibitory compounds co-generated with sugars during pretreatment of LCBs. Among these inhibitory compounds, furfural and 5-(hydroxymethyl)furfural (HMF) are the most abundant, and thus, contribute the most toxicity to LCB hydrolysates. To address this challenge, we engineered a strain of <em>Pseudomonas putida</em> DSM 6125 to efficiently utilize furfural and HMF as carbon sources, without consuming glucose, the major sugar derived from LCB pretreatment and hydrolysis. <em>P</em>. <em>putida</em> DSM 6125 is innately incapable of utilizing xylose, the second most abundant sugar in LCB hydrolysates. To engineer a strain that selectively consumes furfural and HMF, the glucose uptake and catabolic genes namely, carbohydrate-selective porin (<em>oprB-II</em>), glucose dehydrogenase (<em>gcd</em>), and glucokinase (<em>glk</em>) were inactivated to obtain <em>P. putida</em>^{<em>oprB-II-/gcd-/glk-</em>} (Δ<em>oprB-II</em>Δ<em>gcd</em>Δ<em>glk</em>). Subsequently, the furfural and HMF catabolic gene clusters of <em>Cupriavidus basilensis</em> DSM 11853 were chromosomally integrated into <em>P. putida</em>^{<em>oprB-II-/gcd-/glk-</em>} resulting in <em>P.</em> putida^Fur−HMF (Δ<em>oprB-II</em>Δ<em>gcd</em>Δ<em>glk</em>⸬<em>hmfFGH'Hmfs</em>⸬<em>hmfABCDEmfs</em>). <em>P</em>. <em>putida</em>^Fur−HMF consumed ∼1.00–3.84 g/L furfural and 1.30–5.04 g/L HMF supplied separately as carbon sources—following adaptation to increasing inhibitor concentrations—without glucose consumption.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107839"},"PeriodicalIF":5.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of nitrogen sources on growth and biochemical composition of Chlorella sorokiniana","authors":"Faiz Ahmad Ansari ,&nbsp;Humeira Hassan ,&nbsp;Khalid Muzamil Gani ,&nbsp;Ismail Rawat ,&nbsp;Sanjay Kumar Gupta ,&nbsp;Faizal Bux","doi":"10.1016/j.biombioe.2025.107832","DOIUrl":"10.1016/j.biombioe.2025.107832","url":null,"abstract":"<div><div>The increasing demand for sustainable bioenergy solutions emphasizes the need of optimizing microalgae cultivation systems to achieve cost-effective and efficient biomass production. The potential of microalgae as a bioenergy feedstock is significantly influenced by nutrient optimization, particularly with respect to nitrogen. This study evaluated the effects of six nitrogen sources, each providing 25 mg N L⁻¹: sodium nitrate (SN), potassium nitrate (PN), ammonium acetate (AA), ammonium nitrate (AN), ammonium sulphate (AS), and urea (U) on growth of <em>Chlorella sorokiniana</em> growth, carbon dioxide sequestration, biochemical composition, and theoretical methane potential (TBMP). The findings indicate that <em>C. sorokiniana</em> cultivated with AA produced the highest biomass at 1.62 ± 0.1 g L⁻¹ and a protein content of 47.2 ± 1.8 %. Conversely, PN resulted in the highest lipid content (25.54 ± 1 %). SN demonstrated the most significant methane potential (421 ± 1.9 mL CH₄ g⁻¹ VS) and the highest CO₂ fixation rate (0.18 g CO₂ L⁻¹ d⁻¹), comparable to AA. These findings indicate that AA and SN present distinct advantages compared to other selected nitrogen sources. Specifically, AA enhances both biomass yield and nutrient content, while SN demonstrates superior capabilities in methane production potential and CO₂ fixation. These results emphasize the necessity of judiciously selecting nitrogen sources to optimize the balance between bioenergy production, nutrient utilization, and economic viability in the microalgae cultivation.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107832"},"PeriodicalIF":5.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing lipid production in oleaginous yeasts for sustainable bioenergy: A review of process parameters, cultivation strategies, and machine learning integration","authors":"Wannapawn Watsuntorn ,&nbsp;Nuttha Chuengcharoenphanich ,&nbsp;Piroonporn Srimongkol ,&nbsp;Ram Prasath Alagappan ,&nbsp;Anina James ,&nbsp;Eldon R. Rene ,&nbsp;Warawut Chulalaksananukul","doi":"10.1016/j.biombioe.2025.107810","DOIUrl":"10.1016/j.biombioe.2025.107810","url":null,"abstract":"<div><div>The depletion of petroleum reserves has intensified the global pursuit of renewable and sustainable alternative fuels. Single-cell oils (SCOs), produced by microorganisms, have emerged as a promising substitute for traditional fossil fuels and other biological sources owing to their renewability, environmental sustainability, energy efficiency, versatility, and potential to reduce carbon emissions and the reliance on finite resources, all while fostering economic growth and innovation. Among the different microbial sources, oleaginous yeasts are particularly notable for their ability to efficiently synthesize lipids. This review examines the key factors influencing lipid synthesis in yeasts, including carbon sources, carbon-to-nitrogen ratio (C/N), nitrogen sources, aeration rate, agitation speed, pH, and temperature. It also explores the various cultivation strategies, such as batch, sequencing batch, fed-batch, and continuous modes, as well as advanced configurations like two-stage batch configuration and two-stage configuration with feed supply, analyzing their respective advantages and limitations. Lastly, the current trend, that is, the application of machine learning in enhancing lipid productivity has been discussed. This review comprehensively summarizes the overall research implications, and seeks to serve as a compendium on lipid production in oleaginous yeasts that also includes the recommendations for future progress in the field.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107810"},"PeriodicalIF":5.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of wood residue blends from the amazon region for decentralized energy recovery and decarbonization: Combustion kinetics, thermodynamics and potential emissions","authors":"Mayara Gabi Moreira ,&nbsp;Pedro Paulo Oliveira Rodrigues ,&nbsp;Lúcia Fernanda Alves Garcia ,&nbsp;Giulia Cruz Lamas ,&nbsp;José Luiz Franciso Alves ,&nbsp;Jean Constantino Gomes da Silva ,&nbsp;Tiago Jose Pires de Oliveira ,&nbsp;Thiago de Paula Protásio ,&nbsp;Edgar A. Silveira","doi":"10.1016/j.biombioe.2025.107827","DOIUrl":"10.1016/j.biombioe.2025.107827","url":null,"abstract":"<div><div>Decentralized energy systems in the Amazon face challenges such as diesel dependence, high transport costs, and limited sustainability. Woody residues from sustainable forest management offer a viable bioenergy alternative, yet combustion kinetics, thermodynamics, and emissions data remain scarce. This study provides a comprehensive thermokinetic assessment of four blends comprising six Amazonian wood residues (<em>Peltogyne lecointei</em>, <em>Erisma uncinatum</em>, <em>Martiodendron elatum</em>, <em>Handroanthus incanus</em>, <em>Dipteryx odorata</em>, and <em>Allantoma decandra</em>) for decentralized energy solutions. Combustion kinetics were assessed through thermogravimetric analysis, isoconversional methods (Friedman, Flynn-Wall-Ozawa, Kissinger-Akahira-Sunose, and Starink), and DAEM approaches (Miura-Maki, Scott, and Three-Parallel). Additionally, combustion indexes, thermodynamic parameters, emissions, and carbon uptake were analyzed. Results highlighted differences in ignition and burnout performance, with some blends proving more efficient for energy recovery, while others exhibited prolonged combustion, affecting char formation and thermal stability. Experimental and predicted mass loss profiles strongly agreed (MAPE&lt;6 %), confirming the kinetic approach's reliability. Enthalpy changes ranged from 112.89 to 441.90 kJ mol⁻¹, while Gibbs free energy values of 163.25–248.55 kJ mol⁻¹ confirmed a non-spontaneous process. Entropy variations from −116.31–431.54 J mol⁻¹ K⁻¹ indicated molecular disorder and energy efficiency during biomass decomposition. Emission factors for CO₂ (67.109–69.773 tons MJ⁻¹), SO₂ (0.043–0.056 tons MJ⁻¹), and NOx (0.008–0.011 tons MJ⁻¹) were lower than fossil fuels. CO₂ uptake (1.677–1.776 tons per ton of biomass) further supports carbon mitigation. Findings align with SDG7 (Affordable and Clean Energy) and SDG13 (Climate Action), promoting bioenergy integration into diesel-dependent systems in remote regions.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107827"},"PeriodicalIF":5.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe-modified catalytic carbons for enhanced CO2 gasification: Influence of carbon source and operating conditions","authors":"D. Chaos-Hernández,&nbsp;N. Latorre,&nbsp;P. Tarifa,&nbsp;E. Romeo,&nbsp;A. Monzón","doi":"10.1016/j.biombioe.2025.107834","DOIUrl":"10.1016/j.biombioe.2025.107834","url":null,"abstract":"<div><div>In this study, we present results of characterization and reactivity of Fe-doped carbonaceous materials during their catalytic gasification with CO₂. The samples include carbons derived from the thermal treatment of lignocellulosic residues pine sawdust (PiDC) and almond shells (AlDC) and a commercial graphite (AG) used for comparison. Iron-supported samples (Fe/PiDC, Fe/AlDC, Fe/AGC) were prepared by impregnating the raw materials (pine sawdust, almond shells and graphite) with a Fe precursor, followed by thermal decomposition under a reducing atmosphere. Characterization results revealed that Fe incorporation significantly influences the textural properties of the resulting carbonaceous materials. Specifically, Fe doping increased defect density and surface roughness while reducing microporosity, particularly in biomass derived carbons, as the Fe content increased. Dynamic gasification tests demonstrated that Fe enhances the reaction rate and lowers the onset temperature. Optimal gasification performance was achieved with intermediate Fe loadings maximizing catalytic efficiency while preventing rapid deactivation of Fe nanoparticles. Within the temperature range of 850–950 °C, nearly complete gasification was achieved, with residual content minimized to 10 % for Fe(4.2 %wt)/AGC, 16 % for Fe(2.4 %wt)/PiDC and 13 % for Fe(3.2 %wt)/AlDC. However, higher Fe loadings and temperatures exceeding 900 °C led to accelerated Fe deactivation due to sintering and oxidation. At CO₂ concentrations below 8.3 %, these adverse effects were mitigated, optimizing the gasification rate. These findings underscore the critical interplay between Fe dispersion, carbon structure and gasification conditions, offering valuable insights for designing efficient Fe-based catalytic systems for CO₂ valorization in sustainable energy applications.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107834"},"PeriodicalIF":5.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the pyrolysis and combustion behavior and product release characteristics of Chinese medicine residue under a nitrogen/oxygen atmosphere","authors":"Zhao Liuyang,&nbsp;Li Jishuo,&nbsp;Xu Kaili","doi":"10.1016/j.biombioe.2025.107824","DOIUrl":"10.1016/j.biombioe.2025.107824","url":null,"abstract":"<div><div>Traditional Chinese medicine residue (CMR) is a solid waste after the decoction of traditional Chinese medicine. Directly burying or burning is a waste of this resource. Most Chinese medicine residues are from plants, so they have great potential for pyrolysis, gasification and combustion. However, research on the CMR pyrolysis combustion process and pyrolysis gasification products is lacking. This study analysed the pyrolysis and combustion characteristics of 14 kinds of CMRs, and studied the variation law of the three-phase products of CMR pyrolysis and gasification under different pyrolysis temperatures and oxygen contents. The purpose of this study was to provide insights into the biomass utilization of CMR. The results show that the pyrolysis characteristics of different kinds of CMRs are similar or even highly overlapping, as are the combustion characteristics. The utilization of mixed CMRs is feasible. Pyrolysis at 700 °C is best in a nitrogen atmosphere, the pyrolysis gas output and the calorific value are the highest. During gasification, with the increased volume fraction of oxygen, the production of solid-phase products gradually decreases, and the calorific value of syngas gradually decreases. Hypoxia helps increase the calorific value of the gas. Generally, the calorific value of pyrolysis gas is between 10 and 13 MJ/Nm³, which has a high utilization value.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107824"},"PeriodicalIF":5.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing industrial waste for the co-production of mannosylerythritol and cellobiose lipids by Ustilago maydis","authors":"André D. Valkenburg,&nbsp;George M. Teke,&nbsp;Eugéne van Rensburg,&nbsp;Robert W.M. Pott","doi":"10.1016/j.biombioe.2025.107812","DOIUrl":"10.1016/j.biombioe.2025.107812","url":null,"abstract":"<div><div><em>Ustilago maydis</em>, the fungus responsible for corn smut disease, produces valuable glycolipid biosurfactants: mannosylerythritol lipids (MELs) and cellobiose lipids (CBLs). These compounds have gained industrial interest due to their surface-active and antimicrobial properties. However, downstream processing challenges have hindered their commercial adoption, especially in the absence of hydrophobic substrates. This study explored the co-production of MELs and CBLs by <em>U. maydis</em> DSM 4500 using pure and waste-derived substrates. Results showed that CBLs were predominantly produced during the exponential growth phase, whereas MELs were favoured in the stationary phase. While acidic (pH 2.6) and nitrogen-limited conditions enhanced glycolipid production, microbial growth was inhibited, reducing overall yields. To overcome this, a two-stage strategy was implemented. Biomass formation was first optimized under neutral pH (6.0) and nitrogen-replete conditions. In the second stage, biomass was transferred to glycolipid-favouring conditions, leading to a 190 % and 108 % increase in MEL and CBL titres, respectively, compared to a single-stage process. Additionally, sugarcane molasses was explored as an alternative carbon source, achieving a final CBL titre of 0.43 g/L. Though relatively low, this represents the first report of CBL production from an industrial waste stream. These findings highlight the potential for sustainable co-production of MELs and CBLs from industrial waste, paving the way for future research on optimizing yields and scalability.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"197 ","pages":"Article 107812"},"PeriodicalIF":5.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}