Biomass & Bioenergy最新文献

{"title":"Cassava's harvest residue biochar from rotary kiln pyrolysis: Effects on sandy loam soil properties and maize growth","authors":"Wenes Ramos Silva ,&nbsp;Roberta Menezes Santos ,&nbsp;Alberto Wisniewski Jr","doi":"10.1016/j.biombioe.2025.108464","DOIUrl":"10.1016/j.biombioe.2025.108464","url":null,"abstract":"<div><div>Cassava shoot system (CSS) biomass is a smart and despised raw material for biochar production. Commonly, cassava roots and their husk are most exploited as feedstock for the thermoconversion process. In this research, we proposed the production of biochar from CSS in a continuous pyrolysis reactor at temperatures of 400 °C, 500 °C, and 600 °C, searching for added value to this agricultural residue and to produce a soil amendment material with good water retention potential. CSS-biochars have their effects tested on sandy loam soil at 25- and 50-ton ha⁻¹ application rates. Considering soil amendment and carbon sequestration purposes of biochars, the volatile matter, fixed carbon, and ash content are important parameters to be considered. Higher temperature produces more stable and less organic functionalized biochars, measured by the O/C values from 0.21 to 0.03 and H/C from 0.96 to 0.46. Biochars’ pH increases directly with the ash content and significantly alters the soil pH, increasing it from 5.0 to 6.0 with BC500C and BC600C at a rate of 50 tons ha⁻¹. At the same application rate, BC400C and BC600C presented an increase in water-holding capacity of 13 % and 20 % compared with the control sample, respectively. Overall, the matric potential view for all application rates only the CSS-biochar produced at 600 °C demonstrated superior water retention compared to the reference control. An important observed aspect is the no significant impact of biochar on the maize germination step in pot experiments.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108464"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Succinic acid pretreatment of corn cob for production of xylooligosaccharides and biomass pellets","authors":"Zhenchang Wang ,&nbsp;Yuejie Qiu ,&nbsp;Youhong Fu ,&nbsp;Dehua Li ,&nbsp;Jiahao Zhuang ,&nbsp;Jianxin Jiang","doi":"10.1016/j.biombioe.2025.108461","DOIUrl":"10.1016/j.biombioe.2025.108461","url":null,"abstract":"<div><div>The comprehensive utilization of lignocellulosic biomass (LCB) is crucial for advancing the circular bioeconomy. This study proposes a green biorefinery method based on succinic acid (SA) hydrothermal pretreatment of corncob (CC) to produce xylooligosaccharides (XOS) and biomass pellet fuel for complete biomass utilization. Under optimal pretreatment conditions (150 °C, 40 min, 0.3 M SA), 52.86 % XOS was achieved with a degree of polymerization (DP) of 2–5. Compared to raw CC, the content of cellulose and lignin in the pretreatment residue increased by 27.6 % and 25.5 %, respectively. This compositional change would improve the pellet performance. Lignin acts as a natural binder in biomass pelletizing. Under thermal compression, it softens and bonds, filling the gaps between particles. Simultaneously, it connects cellulose microcrystals through van der Waals forces. Furthermore, the increased crystalline cellulose facilitated mechanical stress dissipation through nanofiber alignment, thereby enhancing pellet density and mechanical strength. Under the pelletizing conditions (80 MPa, 100 °C, 12 % moisture), pellets prepared from pretreated residues showed an 11.99 % increase in density, a 155.78 % rise in compressive strength, a 16.43 % gain in higher heating value (HHV), and a 37.66 % reduction in ash content. The mechanical strength and thermal properties of biomass pellets derived from CC pretreated with SA were significantly enhanced. This environmentally friendly and mild acid pretreatment approach provides an innovative pathway for the efficient, closed-loop utilization of LCB.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108461"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low input pretreatment of halophyte lignocellulose for high enzymatic convertibility","authors":"Stanislav Rudnyckyj ,&nbsp;Mette Hedegaard Thomsen","doi":"10.1016/j.biombioe.2025.108482","DOIUrl":"10.1016/j.biombioe.2025.108482","url":null,"abstract":"<div><div>The present study explores an environmentally friendly alternative for the pretreatment of halophyte lignocellulose by utilizing a novel soaking-extraction cycle. Compositional analysis of <em>Salicornia ramosissima</em> lignocellulose confirmed its high polysaccharide content, ranging from 41 to 55 g/100 g dry matter of halophyte lignocellulose. The soaking-extraction process, enriched with natural plant derived solvent (NPDS), functioned as a natural catalyst, significantly enhancing enzymatic saccharification. A double soaking-extraction cycle resulted in a 90 % hydrolysis yield and an significant 64 g of released sugars/L of hydrolysate, surpassing conventional methods. Nearly 50 % of lignin and 60 % of hemicellulose were removed, effectively reducing the lignin barrier and improving enzyme accessibility. In addition to pretreatment optimization, this study evaluated natural alternatives to synthetic buffers for enzymatic saccharification. Halophyte juice and its variants demonstrated promising potential as buffer alternatives, with juice slurry achieving a hydrolysis yield comparable to synthetic acetate buffer, yielding 37 g of released sugars/L with slurry and 42 g/L with acetate buffer, respectively. Overall, this study introduces a cost-effective and circular approach for halophyte-based biorefineries by integrating a dual-function soaking-extraction cycle that enables both bioactive compound extraction and lignocellulose pretreatment. The findings highlight significant potential for industrial applications, reducing reliance on hazardous chemicals and energy-intensive methods.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108482"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic optimization of paper mulberry silage: Engineered Lacticaseibacillus paracasei-Lactiplantibacillus plantarum consortium with enzymes and industrial by-products for stable biomass preservation","authors":"Jipeng Tian ,&nbsp;Mudasir Nazar ,&nbsp;Xin Wang ,&nbsp;Siran Wang ,&nbsp;Wenjie Zhang ,&nbsp;Chenglong Ding ,&nbsp;Beiyi Liu","doi":"10.1016/j.biombioe.2025.108475","DOIUrl":"10.1016/j.biombioe.2025.108475","url":null,"abstract":"<div><div>Unpredictable natural microbial communities drive suboptimal fermentation in paper mulberry (<em>Broussonetia papyrifera</em>) silage, resulting in protein degradation and elevated ammonia emissions, limiting its utility as a stable biomass resource. A formulated lactic acid bacteria (LAB) consortium (<em>Lactiplantibacillus plantarum</em> and <em>Lacticaseibacillus paracasei</em> at 2:1 ratio) was engineered to replace spontaneous fermentation. This strategy is further optimized by comparing the combination effects of industrial by-products (molasses, M; rice husk powder, RHP; corn cob powder, CCP) and enzymes (fibrolytic enzymes, CE; tannase, DE) with LAB on the fermentation quality, chemical composition, and bacterial community of paper mulberry silage. Results showed that all additives lowered pH values, reduced ammonia nitrogen/total nitrogen ratio (AN/TN) and anti-nutritional tannins, while increasing the beneficial flavonoid contents compared to untreated silage (CON). Bacterial community analysis revealed that the LAB was successfully established, significantly increasing the relative abundance of <em>Lacticaseibacillus</em> within the silage microbiome compared to CON. This microbial community engineering correlated with superior fermentation: a significantly lower pH and reduced AN/TN ratio. Functional prediction using PICRUSt2 indicated LAB treatment, particularly with molasses (LAB_M), specifically upregulated carbohydrate metabolism pathways (‘Fructose and mannose metabolism’, ‘Galactose metabolism’). Synergistic integration of the LAB with CE, DE, or M significantly enhanced fiber degradation (reduced neutral detergent fiber, except for M), and preserved more crude protein and starch than RHP and CCP. This study establishes that targeted LAB engineering constitutes an effective bioengineering strategy for stabilizing paper mulberry biomass. Furthermore, the LAB has broader applicability, potentially enhancing biomass conversion efficiency across diverse feedstock sources.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108475"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the lignocellulose-degrading potential of a novel Talaromyces endophyticus through enzymatic hydrolysis and transcriptomic analysis","authors":"Yixin Sun ,&nbsp;Qi Sun ,&nbsp;Yingying Hou ,&nbsp;Chunying Li ,&nbsp;Xiaomei Hu","doi":"10.1016/j.biombioe.2025.108474","DOIUrl":"10.1016/j.biombioe.2025.108474","url":null,"abstract":"<div><div>Filamentous fungi are key producers of cellulolytic and hemicellulolytic enzymes (CH enzymes), which play pivotal roles in lignocellulosic biomass conversion. Although several <em>Talaromyces</em> sp. have demonstrated the ability to produce CH enzymes, the enzymatic potential and regulatory mechanisms of <em>Talaromyces endophyticus</em> remain poorly understood. This study to systematically investigates the lignocellulose-degrading potential of <em>T</em>. <em>endophyticus</em>, providing new insights into its regulatory networks. The novel strain <em>T</em>. <em>endophyticus</em> NEAU-6 exhibited high CH enzymes activities when cultured on corn stover. Notably, enzymatic hydrolysis of alkali-pretreated corn stover using a crude CH enzyme loading of only 10 FPase/g of substrate resulted in a glucose yield of 70.2 %, highlighting its efficiency at a relatively low enzyme dosage. Structural and compositional characterization confirmed efficient degradation of the biomass. Full-length and comparative transcriptomic analyses uncovered 54 upregulated CH enzyme genes and a regulatory network involving 11 canonical transcription factors and developmental regulators, alongside 15 sugar transporters potentially mediating carbon sensing and uptake. These findings elucidate the regulatory network of lignocellulose degradation in <em>T. endophyticus</em> and highlight its potential as a candidate for further development in enzyme production and biomass conversion.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108474"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixotrophic co-cultures fermentation of C1-gases and carrot discard hydrolysate by C. carboxidivorans and C. beijerinckii in stirred tank bioreactor and gas-lift bioreactor","authors":"Juan Carlos López-Linares ,&nbsp;Marina Fernández-Delgado ,&nbsp;Cristina Betanzos-Salguero ,&nbsp;María Ángeles Herrero-Vieira ,&nbsp;Susana Lucas ,&nbsp;Mónica Coca ,&nbsp;M. Teresa García-Cubero","doi":"10.1016/j.biombioe.2025.108473","DOIUrl":"10.1016/j.biombioe.2025.108473","url":null,"abstract":"<div><div>The current study represents the bioconversion of C1 gases (CO and CO₂) using co-cultures of <em>Clostridium carboxidivorans</em> and <em>Clostridium beijerinckii</em>, with hydrolysates from carrot discard as a co-substrate and supplemental Fe⁰. The performance of two distinct bioreactor configurations, a stirred-tank bioreactor (STB) and a gas-lift bioreactor (GLB) was compared under various gas flow rates. The GLB technology, operating at a gas flow of 50 mL/min, proved more efficient, yielding up to 12.0 g/L of butanol, 2.7 g/L of acetic acid, and 7.4 g/L of butyric acid within a 47 h fermentation period. This process achieved complete consumption of sugars and CO, alongside a maximum CO₂ uptake of 82.4 % at t = 33 h. Consequently, the gas-lift bioreactor represents a promising strategy for the co-fermentation of C1 gases and carrot discard hydrolysate by <em>C. carboxidivorans</em> and <em>C. beijerinckii</em>, offering low energy requirements, cost-effectiveness, and simplicity in design and operation.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108473"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing high-value biomass in Scenedesmus and Chlorella: Exploring light-driven macromolecular storage for nutraceutical and feed applications","authors":"P. Scarponi ,&nbsp;F. Chiavolelli ,&nbsp;L. Arru ,&nbsp;L. Forti","doi":"10.1016/j.biombioe.2025.108485","DOIUrl":"10.1016/j.biombioe.2025.108485","url":null,"abstract":"<div><div>The stringent safety requirements for using microalgal biomass in the pharmaceutical, food, and feed industries demand increased scientific research to identify optimal cultivation conditions under axenic environments. To achieve high biomass yields and macromolecular productivity, various cultivation parameters must be systematically evaluated. In particular, the strain-specific responses to these conditions underscore the importance of understanding how inorganic carbon supply and light irradiation influence biomass proliferation and macromolecular accumulation. To gain deeper insight into microalgae productivity, <em>Scenedesmus</em> sp. and <em>Chlorella</em> sp. were cultivated under axenic condition with a 2 % CO₂ supply. The effects of different light wavelengths (red, green and blue) and light intensities (ranging from 80 μE to 200 μE) were investigated. The highest biomass production for <em>Scenedesmus</em> sp. was observed under red, green, and blue light at an intensity of 200 μE, yielding 0.78 ± 0.12 gL^-1, 0.94 ± 0.07 g L⁻¹, and 1.00 ± 0.10 g L⁻¹, respectively. Red, green, and blue light at 150 μE intensity increased lipid storage to 25.32 ± 1.50 %, 39.36 ± 1.81 %, and 33.91 ± 19.87 %, respectively. In contrast, the biomass of <em>Chlorella</em> remained stable under 100 μE of red, green, and blue light, producing 0.65 ± 0.04 g L⁻¹, 0.66 ± 0.09 g L⁻¹, and 0.66 ± 0.07 g L⁻¹, respectively. Increasing light intensity did not significantly affect lipid storage, which remained at 6.87 %. Protein content was consistent in both microalgae strains across all experimental conditions, exceeding 15 %. Lipid characterization revealed a high concentration of oleic, palmitic, stearic, and vaccenic acids, highlighting the potential for integrating these microalgae strains into the pharmaceutical, nutraceutical and feed industries.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108485"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic activation-doping strategy for constructing N/O/B/S Co-doped hierarchical porous carbon from wild jujube pit towards high-performance supercapacitors","authors":"Xu Liu ,&nbsp;Zewang Liu ,&nbsp;Haojie Chen ,&nbsp;Guang zai Nong","doi":"10.1016/j.biombioe.2025.108466","DOIUrl":"10.1016/j.biombioe.2025.108466","url":null,"abstract":"<div><div>This study developed a synergistic activation-doping strategy using Wild Jujube Pits as a carbon source. The K₂CO₃/(NH₄)₂SO₄/H₃BO₃ ternary system synchronously modulates pore evolution and heteroatom doping, successfully constructing N/O/B/S-co-doped hierarchical porous carbon (JS1211). Characterisation results demonstrate that JS1211 possesses a high SSA of 1496 m² g⁻¹ with hierarchical porosity (micropores: 0.4–0.8 nm; mesopores: 2–10 nm). XPS analysis confirms uniform co-doping of N (7.55 at%), B (6.04 at%), S (0.56 at%), and O (15.78 at%), where pyridinic-N (49.4 %) and BC₂O (43.6 %) configurations dominate. DFT calculations reveal a three-tiered doping mechanism proceeding through structural distortion → electronic reconstruction → property optimisation. Electrochemical testing shows JS1211 delivers a specific capacitance of 411 F g⁻¹ at 0.5 A g⁻¹, with 81.75 % capacity retention at 20 A g⁻¹ and 97.31 % capacitance retention after 5000 cycles (Coulombic efficiency: 100.46 %). The assembled symmetric supercapacitor achieves an energy density of 12.85 Wh kg⁻¹ (Power density: 129.71 W kg⁻¹) in a 6 M KOH electrolyte, while retaining 7.85 Wh kg⁻¹ at a power density of 10,335 W kg⁻¹. This work provides new insights for developing biomass-derived high-performance supercapacitor electrodes.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"205 ","pages":"Article 108466"},"PeriodicalIF":5.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syngas production from biomass pyrolysis followed by in-line biochar-catalytic reforming: the effect of space velocity, particle size, and morphology","authors":"Hanmin Yang ,&nbsp;Yazhe Wang ,&nbsp;Yanghao Jin ,&nbsp;José Juan Bolívar Caballero ,&nbsp;Shiwei Chen ,&nbsp;Ziyi Shi ,&nbsp;Tong Han ,&nbsp;Linda Sandström ,&nbsp;Pär G. Jönsson ,&nbsp;Weihong Yang","doi":"10.1016/j.biombioe.2025.108481","DOIUrl":"10.1016/j.biombioe.2025.108481","url":null,"abstract":"<div><div>A continuous pyrolysis combined with an in-line biochar-catalytic reforming of the pyrolysis vapor was investigated in a comprehensive system consisting of an auger reactor and a downstream fixed-bed reactor. The effect of the weight hourly space velocity (WHSV), particle size and morphology of biochar, and the pressure drop of the biochar bed on the catalytic performance were discussed in this study. Results showed that lower WHSV, which allows longer residence time, led to higher syngas yield and increased H₂+CO proportion. The use of the smallest biochar particles (0.6–1 mm) produced the highest syngas and H₂ yields, correlating with the greatest pressure drops. Spherical and rounded biochar particle shape enhanced syngas and H₂ yields, as well as H₂+CO proportions, due to improved heat and mass transfer. A maximum of 12 mmol H₂/g-biomass was achieved, with a dry gas yield of 0.68 Nm³/kg, comprising 39 vol % H₂ and 27 vol % CO, at the use of pelletized biochar with a WHSV of 0.51. The used biochar demonstrated stable catalytic performance as a reforming catalyst in a 100-min test period.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"204 ","pages":"Article 108481"},"PeriodicalIF":5.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Agave sisalana waste into high-yield biogas: An approach to sustainable energy","authors":"Fabiane M. Vieira ,&nbsp;Maiki S. de Paula ,&nbsp;Maria Paula C. Volpi ,&nbsp;Oscar F. Herrera Adarme ,&nbsp;Thiago Ribas ,&nbsp;Jean C.G. Silva ,&nbsp;Marcelo F. Carazzolle ,&nbsp;Gonçalo A.G. Pereira ,&nbsp;Gustavo Mockaitis","doi":"10.1016/j.biombioe.2025.108476","DOIUrl":"10.1016/j.biombioe.2025.108476","url":null,"abstract":"<div><div>Agave, a native Mexican plant widely used for fiber extraction and alcoholic beverage production, has significant potential for biogas generation. Brazil, the global leader in sisal fiber production, extracts fiber from only 4 % of the agave leaf, leaving approximately 24 tons of organic residue per ton of fiber. These residues can be converted into methane (CH₄) through anaerobic digestion (AD). This study explores biogas production from agave fiber extraction residues. The juice and pulp contain high levels of calcium, potassium, magnesium (2–3 gL^-1), citric acid (56 g L⁻¹), and formic acid (76 g L⁻¹). Methane potentials were 476 NmLCH₄ gVS⁻¹ for the juice and 331.10 NmLCH₄ gVS⁻¹ for the pulp. Anaerobic digestion revealed microbial groups such as <em>Anaerolineae</em>, <em>Synergistia</em>, <em>Clostridia</em>, <em>Bacteroidia</em>, and <em>Gammaproteobacteria</em>, which degrade carbohydrates and volatile fatty acids. <em>Halobacteriota</em>, a methane-producing archaea, predominated in all samples. Energy recovery scenarios considering raw sisal pulp direct AD (1) and an integrated biorefinery proposal (2) suggests that 1034 MJ of energy can be obtained per ton of biomass, reaching 1951 MJ when integrated into a biorefinery. This study emphasizes the value of agave defibration residues, typically discarded, and proposes an AD-based biorefinery model to enhance the sustainability of sisal fiber production.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"204 ","pages":"Article 108476"},"PeriodicalIF":5.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}