Quality valuation and open fermentation process of polyhydroxyalkanoates production for the waste carbons biorefinery using Paracoccus sp. TOH as chassis

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING

Biomass & Bioenergy Pub Date : 2025-05-17 DOI:10.1016/j.biombioe.2025.107990

Leizhen Zhao, Lin Chen, Yuanxi Zhang, Jiexuan Han, Gaojie Sun, Jianhang Qu

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引用次数: 0

Abstract

Waste materials with sufficient carbon substrate and deficient nitrogen content are optimal for microbial synthesis of polyhydroxyalkanoates (PHA), particularly in an open fermentation model using piperazine as a nitrogen-based controlling element. This study evaluated the fermentation performance of different waste carbons (by-product sodium acetate, crude glycerol, and cane molasses) using a versatile strain, Paracoccus sp. TOH. Firstly, it was observed that crude glycerol has a higher carbon content (1589.9 g-COD·L⁻¹, 551.1 ± 4.6 g-TOC·L⁻¹), a lower nitrogen proportion (1.61 g L⁻¹), and a favorable price (125 $·t⁻¹). Subsequently, crude glycerol was identified as a suitable substrate for poly(3-hydroxybutyrate) (PHB) fermentation. The optimal PHB concentration was found to be 1.99 g L⁻¹ and 1.81 g L⁻¹ for (NH₄)₂SO₄ and piperazine system, respectively, after process debugging. Foremost, the feasibility of open PHB accumulation (0.87 g L⁻¹) was achieved by dynamically adjusting the feed of piperazine. This study provided valuable insights into the development of a convenient biomanufacturing technique for the valorization of organic waste.

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以TOH副球菌为底料的废炭生物精炼厂生产聚羟基烷酸酯的质量评价及开放式发酵工艺

具有足够碳底物和缺乏氮含量的废物是微生物合成聚羟基烷酸酯（PHA）的最佳材料，特别是在使用哌嗪作为氮基控制元素的开放式发酵模型中。本研究利用一种多功能菌株副球菌sp. TOH，评估了不同废碳（副产物乙酸钠、粗甘油和甘蔗糖蜜）的发酵性能。首先，粗甘油具有较高的含碳量（1589.9 g- cod·L−1,551.1±4.6 g- toc·L−1），较低的含氮量（1.61 g L−1）和较优惠的价格（125美元·t−1）。随后，粗甘油被确定为聚3-羟基丁酸酯（PHB）发酵的合适底物。经过工艺调试，发现（NH4）2SO4和哌嗪体系的最佳PHB浓度分别为1.99 g L−1和1.81 g L−1。首先，通过动态调节哌嗪的进给量，实现了开放积累PHB （0.87 g L−1）的可行性。该研究为开发一种方便的有机废物增值生物制造技术提供了有价值的见解。

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.