Biomedical Microdevices最新文献

Flexible porous microneedle array for bioelectric skin patch 用于生物电皮肤贴片的柔性多孔微针阵列

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-05-10 DOI: 10.1007/s10544-025-00749-y

Soichiro Tottori, Mirai Matsuura, Sae Ichinose, Haechang Cho, Tarryn Galloway, Natsuho Moriyama, Matsuhiko Nishizawa

引用次数: 0

Cyanocobalamin-loaded dissolving microneedles for enhanced transdermal delivery: development, characterization, and pharmacokinetic evaluation 用于增强透皮给药的氰钴胺负载溶解微针：开发、表征和药代动力学评价

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-05-01 DOI: 10.1007/s10544-025-00747-0

Mousam Bhowmik, Rajamma A. J., Sateesha S. B., Chandan R. S., Girija E. K., Punith M, Ebna Azizal Omar, Rajesh R

{"title":"Cyanocobalamin-loaded dissolving microneedles for enhanced transdermal delivery: development, characterization, and pharmacokinetic evaluation","authors":"Mousam Bhowmik, Rajamma A. J., Sateesha S. B., Chandan R. S., Girija E. K., Punith M, Ebna Azizal Omar, Rajesh R","doi":"10.1007/s10544-025-00747-0","DOIUrl":"10.1007/s10544-025-00747-0","url":null,"abstract":"<div><p>This study demonstrates cyanocobalamin-loaded dissolving microneedles (CNBL-MNs) as a minimally invasive transdermal solution for managing cyanocobalamin (CNBL) deficiency, offering an alternative to intramuscular injections and oral supplements. The CNBL-MNs were developed using biodegradable, water-soluble polymers such as polyvinylpyrrolidone K25, Dextran K40, and chitosan to ensure controlled and gradual release of the CNBL. The formulation’s stability and integrity were assessed through FTIR and XRD analyses. SEM imaging revealed well-formed microneedles with a height of 800 μm, a 200 μm base diameter, and a 500 μm pitch. EDS confirmed the successful incorporation of CNBL in the microneedle array. The Parafilm<sup>®</sup> membrane insertion test revealed that the microneedles had strong mechanical properties and achieved 100% penetration efficiency. The microneedle array also demonstrated excellent (<i>P</i> > 0.05) flexibility and structural stability. Ex-vivo release studies showed that 88.51% of the CNBL was released over 48 h, following a first-order kinetic model. The <i>n</i> value of 0.51 for Korsmeyer-Peppas model indicate an anomalous transport mechanism, suggesting a combination of diffusion and erosion. The in-vivo pharmacokinetic evaluation in Wistar rats demonstrates that CNBL-MNs-2 exhibited a larger area under the curve (AUC₀–t) (61.57 ± 4.23 ng·h/mL) than the IP injection (37.04 ± 5.83 ng·h/mL), indicating significant (<i>p</i> > 0.05) increase in systemic availability and sustained release. The Cmax of CNBL-MNs-2 (6.10 ± 0.533 ng/mL) was comparable to that of the IP injection (6.20 ± 1.5 ng/mL), confirming efficient systemic absorption <i>via</i> the microneedle system. Additionally, Tmax was significantly (<i>p</i> > 0.05) prolonged with CNBL-MNs-2 (8 h) compared to the IP injection (2 h), suggesting a slower, more controlled CNBL release.</p></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing glaucoma care with smart contact lenses: An overview of recent developments 用智能隐形眼镜加强青光眼护理：近期发展综述

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-04-21 DOI: 10.1007/s10544-025-00740-7

Ali Fardoost, Koosha Karimi, Jaydeep Singh, Heneil Patel, Mehdi Javanmard

{"title":"Enhancing glaucoma care with smart contact lenses: An overview of recent developments","authors":"Ali Fardoost, Koosha Karimi, Jaydeep Singh, Heneil Patel, Mehdi Javanmard","doi":"10.1007/s10544-025-00740-7","DOIUrl":"10.1007/s10544-025-00740-7","url":null,"abstract":"<div><p>Glaucoma is a leading cause of irreversible blindness worldwide, affecting millions of individuals due to its progressive damage to the optic nerve, often caused by elevated intraocular pressure (IOP). Conventional methods of IOP monitoring, such as tonometry, provide sporadic and often inaccurate readings due to fluctuations throughout the day, leaving significant gaps in diagnosis and treatment. This review explores the transformative potential of smart contact lenses equipped with continuous IOP monitoring and therapeutic capabilities. These lenses integrate advanced materials such as graphene, nanogels, and magnetic oxide nanosheets alongside sophisticated biosensing and wireless communication systems. By offering continuous, real-time data, these lenses can detect subtle IOP fluctuations and provide immediate feedback to patients and clinicians. Moreover, drug-eluting capabilities embedded in these lenses present a groundbreaking approach to glaucoma therapy by improving medication adherence and providing controlled drug release directly to the eye. Beyond IOP management, these innovations also pave the way for monitoring biochemical markers and other ocular diseases. Challenges such as biocompatibility, long-term wearability, and affordability remain, but the integration of cutting-edge technologies in smart contact lenses signifies a paradigm shift in glaucoma care. These developments hold immense promise for advancing personalized medicine, improving patient outcomes, and mitigating the global burden of blindness.</p></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10544-025-00740-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Simulation of advanced boron-doped GaN cap layer on AlGaN/GaN MOSHEMTs for enhanced label-free biosensing applications 在 AlGaN/GaN MOSHEMT 上设计和模拟先进的掺硼 GaN 盖层，用于增强型无标记生物传感应用

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-04-21 DOI: 10.1007/s10544-025-00746-1

Reddy Govindappagari Hemalatha, Manoharan Arun Kumar, Girish Shankar Mishra, MohanKumar N, Kamal Batcha Mohamed Ismail, Shanmugam Mahalingam, Junghwan Kim

{"title":"Design and Simulation of advanced boron-doped GaN cap layer on AlGaN/GaN MOSHEMTs for enhanced label-free biosensing applications","authors":"Reddy Govindappagari Hemalatha, Manoharan Arun Kumar, Girish Shankar Mishra, MohanKumar N, Kamal Batcha Mohamed Ismail, Shanmugam Mahalingam, Junghwan Kim","doi":"10.1007/s10544-025-00746-1","DOIUrl":"10.1007/s10544-025-00746-1","url":null,"abstract":"<div><p>This study focuses on the design and simulation of a biosensor based on HEMT technology, with a focus on a GaN/AlGaN MOSHEMT architecture with a cavity and a boron-doped GaN cap layer, for identifying label-free biological molecules. The inclusion of a boron-doped GaN cap layer in the AlGaN/GaN heterostructure facilitates E-mode operation. We examined the influence of neutral or label-free biomolecules on the electron concentration and device sensitivity. The Sentaurus TCAD device simulation tool was used to analyze the MOSHEMT structure. Our findings suggest that low dielectric biomolecules increase the drain current, whereas higher dielectric values decrease the drain current. We also evaluated the device performance across various cavity lengths (100 nm, 200 nm, 300 nm, and 400 nm). The AlGaN/GaN MOSHEMT provides excellent sensitivity and precision in biological detection. The proposed GaN cap layer MOSHEMT biosensor is designed to detect biomolecules such as Keratin, Zein, ChOx, Biotin, Streptavidin, and Urease. The addition of these biomolecules to the nanocavity significantly enhances the drain current, transconductance (g<sub>m</sub>), output conductance (g<sub>d</sub>), and sensitivity. The device demonstrates high sensitivity (~ 73%) under optimized parameters, making it suitable for precise label-free biosensing applications.</p></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-site analysis of cortisol in saliva based on microchannel lateral flow assay (mLFA) on polymer lab-on-a-chip (LOC) 基于聚合物芯片实验室（LOC）微通道横向流动测定（mLFA）的唾液皮质醇现场分析

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-04-10 DOI: 10.1007/s10544-025-00733-6

V. Thiyagarajan Upaassana, Supreeth Setty, Heeyeong Jang, Sthitodhi Ghosh, Chong Ahn

{"title":"On-site analysis of cortisol in saliva based on microchannel lateral flow assay (mLFA) on polymer lab-on-a-chip (LOC)","authors":"V. Thiyagarajan Upaassana, Supreeth Setty, Heeyeong Jang, Sthitodhi Ghosh, Chong Ahn","doi":"10.1007/s10544-025-00733-6","DOIUrl":"10.1007/s10544-025-00733-6","url":null,"abstract":"<div><p>Unbound cortisol in saliva, detectable through non-invasive sampling, is widely recognized as a validated biomarker for the biochemical evaluation of common mental disorders such as chronic stress, depression, anxiety, and post-traumatic stress disorder (PTSD). In this work, we report a novel polymer lab-on-a-chip (LOC) for microfluidic lateral flow assay (mLFA) with on-chip dried reagents for the detection of unbound cortisol in saliva using a competitive immunoassay protocol. The new polymer microchannel lateral flow assay on lab-on-a-chip (mLFA-LOC), replicated using injection molding technology, are composed of sequentially connected microchannels for sample loading, detection antibody immobilization, flow delay, sensing spirals for test and control, and a capillary pump at the end. The competitive immunoassay of cortisol can be autonomously performed through the microchannels after sample loading of the filtered saliva, and the fluorescence signals emitted from the sensing spirals are detected and quantified by a custom-designed, portable fluorescence analyzer developed in this work. For the evaluation of cortisol assay, artificial saliva samples spiked with unbound cortisol were analyzed using mLFA-LOC and the portable analyzer. The performed competitive assay of unbound cortisol showed a limit of detection (LoD) of 1.8 ng/mL and an inter-chip coefficient of variation (CV) of 4.0%, which covers the clinical range for on-site unbound salivary cortisol analysis. The newly developed mLFA-LOC platform certainly works successfully for the rapid on-site sampling and analysis of salivary biomarkers.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10544-025-00733-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel glass chip based lateral flow immunoassay of albumin 一种新型的基于玻璃芯片的白蛋白横向流动免疫分析方法

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-03-26 DOI: 10.1007/s10544-025-00744-3

Xuanxu Nan, Yiyang Wu, Lingyi Xu, Li Yang, Yue Cui

引用次数: 0

A capillary-driven microfluidic device for performing spatial multiplex PCR 一种用于执行空间多重PCR的毛细管驱动微流控装置

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-03-26 DOI: 10.1007/s10544-025-00745-2

Rodrigo S. Wiederkehr, Elisabeth Marchal, Maarten Fauvart, Tomas Forceville, Ahmed Taher, Tim Steylaerts, YoungJae Choe, Hans Dusar, Silvia Lenci, Eleni Siouti, Vassiliki T. Potsika, Evangelos Andreakos, Tim Stakenborg

{"title":"A capillary-driven microfluidic device for performing spatial multiplex PCR","authors":"Rodrigo S. Wiederkehr, Elisabeth Marchal, Maarten Fauvart, Tomas Forceville, Ahmed Taher, Tim Steylaerts, YoungJae Choe, Hans Dusar, Silvia Lenci, Eleni Siouti, Vassiliki T. Potsika, Evangelos Andreakos, Tim Stakenborg","doi":"10.1007/s10544-025-00745-2","DOIUrl":"10.1007/s10544-025-00745-2","url":null,"abstract":"<div><p>Multiplex polymerase chain reaction (PCR) tests multiple biomarkers or pathogens that cause overlapping symptoms, making it an essential tool in syndromic testing. To achieve a multiplex PCR on chip, a design based on capillary-driven fluidic actuation is proposed. Our silicon chip features 22 reaction chambers and allows primers and probes to be pre-spotted in the reaction chambers prior to use. The design facilitates rapid sample loading through a common inlet channel, delivering reagents to all reaction chambers in less than 10 s. A custom clamping mechanism combined with a double depth cavity design ensures proper sealing during temperature cycling without the need for extra reagents like oil. Temperature cycling and fluorescence imaging were performed using custom-made hardware. As a proof of concept, two single nucleotide polymorphisms (SNPs), CyP2C19*2 and PCSK9 were detected. These results demonstrate the feasibility of on-chip multiplex PCR, compatible with different assays in parallel and requiring only a single pipetting step for reagent loading, without active fluidic actuation like pumping.</p></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10544-025-00745-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New intraocular lens containing a drug delivery system (IOL-DDS) loaded with dexamethasone 新型人工晶状体含有一种装载地塞米松的药物传递系统（IOL-DDS）

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-03-24 DOI: 10.1007/s10544-025-00743-4

Brenda F. M. Castro, Raquel G. Arribada, Thomas T. Inoue, Elias R. Filho, Bruno C. Sena, Luiz F. L. Ferreira, Silvia L. Fialho, Armando Silva-Cunha

{"title":"New intraocular lens containing a drug delivery system (IOL-DDS) loaded with dexamethasone","authors":"Brenda F. M. Castro, Raquel G. Arribada, Thomas T. Inoue, Elias R. Filho, Bruno C. Sena, Luiz F. L. Ferreira, Silvia L. Fialho, Armando Silva-Cunha","doi":"10.1007/s10544-025-00743-4","DOIUrl":"10.1007/s10544-025-00743-4","url":null,"abstract":"<div><p>This study demonstrates the development of polymeric PLGA (50:50) nanoparticles containing dexamethasone acetate, which are dispersed in a PVA film and added to hydrophobic intraocular lenses (IOL) exclusively designed for this application. The resulting IOL-drug delivery system (IOL-DDS) can be introduced into the eye with syringe-type injectors and standard surgical techniques. The obtained results showed that the lens design does not compromise stability within the eye or weaken the loops, preserves its optical zone, and maintains injector’s functionality during surgery. The IOL-DDS releases the drug in vivo for 7 days within the therapeutic concentration range. Short-term assessment confirms the safety of the developed device for ocular structures, which is supported by slit lamp observations, intraocular pressure measurements, optical coherence tomography, and histological analysis. Minor changes in specular microscopy parameters are observed and may be related to the use of IOL and surgical instruments designed for human eyes in smaller rabbit eyes.</p></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A spiral channel with integrated microelectrodes for label-free particle lateral position and size characterization 集成微电极的螺旋通道，用于无标记颗粒横向位置和尺寸表征

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-03-18 DOI: 10.1007/s10544-025-00742-5

Yunhao Peng, Bruce K. Gale, Himanshu J. Sant

{"title":"A spiral channel with integrated microelectrodes for label-free particle lateral position and size characterization","authors":"Yunhao Peng, Bruce K. Gale, Himanshu J. Sant","doi":"10.1007/s10544-025-00742-5","DOIUrl":"10.1007/s10544-025-00742-5","url":null,"abstract":"<p>Modified-trident shaped microelectrodes were incorporated into a spiral-shaped microfluidic focusing channel, utilizing impedance flow cytometry to analyze and quantify inertial microfluidic-based separation of homogeneous particles differing in size. Double peak voltage pulses were generated as particles moved across the electrodes, where the ratio of the peak amplitudes indicated the lateral particle positions inside the channel at various flow rates, while the peak amplitude indicated particle size and vertical position. The root mean square error between the optical and electrical position measurements was 11.44 µm reflecting the lateral position measurement resolution. The peak amplitudes were used to estimate particle size after being adjusted to account for particle vertical position using a shape parameter, which effectively reduced errors in particle size calculations. The particle size estimate sensitivity was measured to be 2.15 μm/mV from the peak amplitudes. The electrodes with the appropriate signal processing were able to detect both the size and location of particles after separation with a spiral channel, showing their utility in potentially controlling the separation conditions for these devices.</p>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lab-on-a-chip device for microfluidic trapping and TIRF imaging of single cells 用于微流体捕获和单细胞TIRF成像的芯片上实验室装置

IF 3 4区医学

Biomedical Microdevices Pub Date : 2025-03-14 DOI: 10.1007/s10544-025-00739-0

Dustin Dzikonski, Riccardo Zamboni, Aniket Bandyopadhyay, Deepthi Paul, Roland Wedlich-Söldner, Cornelia Denz, Jörg Imbrock

{"title":"Lab-on-a-chip device for microfluidic trapping and TIRF imaging of single cells","authors":"Dustin Dzikonski, Riccardo Zamboni, Aniket Bandyopadhyay, Deepthi Paul, Roland Wedlich-Söldner, Cornelia Denz, Jörg Imbrock","doi":"10.1007/s10544-025-00739-0","DOIUrl":"10.1007/s10544-025-00739-0","url":null,"abstract":"<p>Total internal reflection fluorescence (TIRF) microscopy is a powerful imaging technique that visualizes the outer surface of specimens in close proximity to a substrate, yielding crucial insights in cell membrane compositions. TIRF plays a key role in single-cell studies but typically requires chemical fixation to ensure direct contact between the cell membrane and substrate, which can compromise cell viability and promote clustering. In this study, we present a microfluidic device with structures designed to trap single yeast cells and fix them in direct contact with the substrate surface to enable TIRF measurements on the cell membrane. The traps are fabricated using two-photon polymerization, allowing high-resolution printing of intricate structures that encapsulate cells in all three dimensions while maintaining exposure to the flow within the device. Our adaptable trap design allows us to reduce residual movement of trapped cells to a minimum while maintaining high trapping efficiencies. We identify the optimal structure configuration to trap single yeast cells and demonstrate that trapping efficiency can be tuned by modifying cell concentration and injection methods. Additionally, by replicating the cell trap design with soft hydrogel materials, we demonstrate the potential of our approach for further single-cell studies. The authors have no relevant financial or non-financial interests to disclose and no competing interests to declare.</p>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10544-025-00739-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0