Detection of EGFR mutations at pM concentration in ten minutes using a microfluidic concentration and separation module

IF 3.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2025-08-28 DOI:10.1007/s10544-025-00767-w

Jeffrey Teillet, Anne Pradines, Naima Hanoun, Jules Edwards, Pierre Joseph, Anne-Marie Gué, Aurélien Bancaud, Pierre Cordelier

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引用次数: 0

Abstract

Epidermal growth factor receptor (EGFR) mutation detection is now commonly used in the management of cancer patients, particularly those diagnosed with non-small cell lung cancer. Molecular beacon-based sensing is direct and rapid, but its sensitivity is low. Conversely, high-sensitivity detection methodologies based on amplification are robust and sensitive but are limited by relatively require long turnaround times. In this study, we utilized a size-resolved, molecular beacon-based strategy for the rapid detection of EGFR genomic alterations, specifically exon 19 deletions and L858R point mutation. This technology combines a concentration and separation module, which allows us to successfully demonstrate the detection of deletions and point mutations of EGFR in five minutes with a mutant allele sensitivity of 10%. The use of a dual-color detection insures fast detection with a reduced risk of false positives. This work represents a first step toward the fast and specific detection of genetic mutations to improve the management of patients with hard-to-treat tumors.

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使用微流体浓度和分离模块在10分钟内检测pM浓度下的EGFR突变

表皮生长因子受体（EGFR）突变检测现在通常用于癌症患者的治疗，特别是那些被诊断为非小细胞肺癌的患者。分子信标传感直接、快速，但灵敏度较低。相反，基于放大的高灵敏度检测方法具有鲁棒性和灵敏度，但受相对需要较长的周转时间的限制。在这项研究中，我们使用了一种基于分子信标的大小分辨策略来快速检测EGFR基因组改变，特别是外显子19缺失和L858R点突变。该技术结合了浓度和分离模块，使我们能够在5分钟内成功检测EGFR的缺失和点突变，突变等位基因敏感性为10%。使用双色检测确保快速检测，降低误报风险。这项工作代表了快速和特异性检测基因突变的第一步，以改善难以治疗的肿瘤患者的管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.