Biodegradation最新文献

{"title":"Isolation, characterization, and mycostimulation of fungi for the degradation of polycyclic aromatic hydrocarbons at a superfund site.","authors":"Joshua Crittenden, Daniel Raudabaugh, Claudia K Gunsch","doi":"10.1007/s10532-024-10106-0","DOIUrl":"10.1007/s10532-024-10106-0","url":null,"abstract":"<p><p>Mycoremediation is a biological treatment approach that relies on fungi to transform environmental pollutants into intermediates with lower environmental burden. Basidiomycetes have commonly been used as the target fungal phylum for bioaugmentation in mycoremediation, however this phylum has been found to be unreliable when used at scale in the field. In this study, we isolated, characterized, and identified potential polycyclic aromatic hydrocarbon (PAH) degrading fungal isolates from creosote-contaminated sediment in the Elizabeth River, Virginia. Our goal was to identify non-basidiomycete PAH degrading fungi. A total of 132 isolates were isolated, of which the overwhelming majority belonged to the phylum Ascomycota. Isolates were screened for their ability to produce known PAH degrading enzymes, particularly laccase and manganese-dependent peroxidases, and to transform model PAH compounds [fluoranthene, phenanthrene, pyrene and benzo(a)pyrene]. Fungal isolates were subsequently biostimulated using complex amendments including chicken feathers, wheat seeds, grasshoppers, and maple saw dust. Following biostimulation, laccase expression and PAH transformation were assessed. The grasshopper amendment was found to yield the highest laccase upregulation improvement with a maximum increase of 18.9% for the Paraphaeosphaeria isolate. The Septoriella and Trichoderma isolates exposed to the chitin-based grasshopper amendment demonstrated an increase in PAH removal. Septoriella sp. increased its transformation of fluoranthene (44%), pyrene (54.2%, and benzo(a)pyrene (48.7%), while there was a 58.3% increase in the removal of benzo(a)pyrene by Trichoderma sp. While the results from this study demonstrate the potential of indigenous fungi to be biostimulated for the removal of PAHs, additional investigation is needed to determine if the response to the chitin-based grasshopper mycostimulation can be translated from the bench to the field.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":"15"},"PeriodicalIF":3.1,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of polyhydroxyalkanoate (PHA) production from biohythane pilot plant effluent by Cupriavidus necator TISTR 1335","authors":"Yu-Ting Song,&nbsp;Napapat Sitthikitpanya,&nbsp;Nikannapas Usmanbaha,&nbsp;Alissara Reungsang,&nbsp;Chen-Yeon Chu","doi":"10.1007/s10532-025-10110-y","DOIUrl":"10.1007/s10532-025-10110-y","url":null,"abstract":"<div><p>Bioplastics, particularly polyhydroxyalkanoates (PHAs), are emerging as promising alternatives to traditional materials due to their biodegradability. This study focuses on the production of PHAs as bioplastics using effluent from hydrogen production in a two-stage Biohythane Pilot Plant, which provides a low-cost substrate. The aim is to optimize production conditions, with <i>Cupriavidus necator</i> TISTR 1335 being used as the PHA producer. Utilizing Response Surface Methodology-Central Composite Design, we explored optimal conditions, revealing peak PHA production at a substrate concentration of 33.51 g COD/L and a pH of 6.87. The predicted optimal PHA concentration was at 3.05 g/L within the established model, closely matching the experimentally validated value of 3.02 g/L, with the overall usage rate of reducing sugars approximately 50–60%. This study underscores the importance of optimizing PHA production conditions and paving the way toward large-scale PHA production.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in waste-derived functional materials for PFAS remediation","authors":"Saba Andleeb,&nbsp;Muhammad Irfan,&nbsp;Emmanuel Atta-Obeng,&nbsp;Dalia Sukmawati","doi":"10.1007/s10532-025-10109-5","DOIUrl":"10.1007/s10532-025-10109-5","url":null,"abstract":"<div><p>Per- and polyfluoroalkyl substances (PFAS) are synthetic organofluoride compounds, widely used in industries since the 1950s for their hydrophobic properties. PFAS contamination of soil and water poses significant environmental and public health risks due to their persistence, chemical stability, and resistance to degradation. The Chemical Abstracts Service catalogs approximately 4300 PFAS globally. Research in various regions such as North America, Asia, Europe, and remote polar zones has revealed the accumulation of perfluorooctane sulfonate (PFOS) in the tissues of various animal species, with concentrations reaching up to 1900 ng/g in aquatic species like dolphins and whales. Researchers have employed various remediation techniques such as solvent extraction, ion exchange, precipitation, adsorption, and membrane filtration, each of which has its drawbacks. Adsorption, particularly using waste-derived functional materials like biochar, is emerging as a promising method for PFAS remediation due to its cost-effectiveness and sustainability. For example, waste timber-derived biochar exhibits adsorption efficiency comparable to commercial activated carbon. This review highlights advancements in using agricultural, industrial, and biological waste-derived materials for sustainable PFAS remediation. We discuss innovative modification techniques like hydrothermal synthesis, pyrolysis, calcination, co-precipitation, the sol–gel method, and ball milling. The study also examines adsorption mechanisms, factors affecting adsorption efficiency, and the technological challenges in scaling up waste-derived material use. It aims to explore developments, challenges, and future directions for using these materials for efficient PFAS remediation and contributing to sustainable environmental cleanup solutions.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the intricate studies on low-density polyethylene (LDPE) biodegradation by Bacillus cereus AP-01, isolated from the gut of Styrofoam-fed Tenebrio molitor larvae","authors":"Krishnamoorthi Akash,&nbsp;Rengasamy Parthasarathi,&nbsp;Rajavel Elango,&nbsp;Subramanian Bragadeeswaran","doi":"10.1007/s10532-024-10107-z","DOIUrl":"10.1007/s10532-024-10107-z","url":null,"abstract":"<div><p>This study aims to investigate the biodegradation potential of a gut bacterial strain, <i>Bacillus cereus</i> AP-01, isolated from <i>Tenebrio molitor</i> larvae fed Styrofoam, focusing on its efficacy in degrading low-density polyethylene (LDPE). The biodegradation process was evaluated through a series of assays, including clear zone assays, biodegradation assays, and planktonic cell growth assessments in mineral salt medium (MSM) over a 28-day incubation period. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were employed to characterize the alterations in LDPE pellets, followed by molecular characterization. Over three months, sterile soil + LDPE pellets were treated with different concentrations of gut bacterial strain. The degradation capabilities were assessed by measuring pH, total microbial counts, carbon dioxide evolution, weight loss, and conducting phase contrast microscopy and mechanical strength tests. Results demonstrated that MSM containing LDPE as a carbon source with gut bacterial strain produced a clear zone and enhanced planktonic cell growth. FTIR analysis revealed the formation of new functional groups in the LDPE, while SEM images displayed surface erosion and cracking, providing visual evidence of biodegradation. Molecular characterization confirmed the strain as <i>Bacillus cereus</i> AP-01 (NCBI Accession Number: OR288218.1). A 10% inoculum concentration of <i>Bacillus cereus</i> AP-01 exhibited increased soil bacterial counts, carbon dioxide evolution, and pH levels, alongside a notable weight loss of 30.3% in LDPE pellets. Mechanical strength assessments indicated substantial reductions in tensile strength (7.81 ± 0.84 MPa), compression (4.92 ± 0.53 MPa), hardness (51.96 ± 5.62 shore D), flexibility (10.62 ± 1.15 MPa), and impact resistance (14.79 ± 0.94 J). These findings underscore the biodegradation potential of <i>Bacillus cereus</i> AP-01, presenting a promising strategy for addressing the global LDPE pollution crisis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive modeling of diazinon residual concentration in soils contaminated with potentially toxic elements: a comparative study of machine learning approaches","authors":"Marzieh Mohammadi Aria,&nbsp;Safar Vafadar,&nbsp;Yousef Sharafi,&nbsp;Abbas Ali Ghezelsofloo","doi":"10.1007/s10532-024-10108-y","DOIUrl":"10.1007/s10532-024-10108-y","url":null,"abstract":"<div><p>The widespread use of pesticides, including diazinon, poses an increased risk of environmental pollution and detrimental effects on biodiversity, food security, and water resources. In this study, we investigated the impact of Potentially Toxic Elements (PTE) including Zn, Cd, V, and Mn on the degradation of diazinon in three different soils. We investigated the capability and performance of four machine learning models to predict residual pesticide concentration, including adaptive neuro-fuzzy inference system (ANFIS), support vector regression (SVR), radial basis function (RBF), and multi-layer perceptron (MLP). We employed a 10-fold cross-validation mechanism to evaluate the models. Moreover, performance validation of selected algorithms through the coefficient of determination (R²), root mean square error (RMSE), mean absolute error (MAE) and mean square error (MSE) confirm that the SVR and ANFIS with lower RMSE, MSE, and a higher R² can simulate the degradation process better than other models. The result showed that both SVR and ANFIS approaches worked well for the data set, but the SVR technique is more accurate than the fuzzy model for estimating pesticide concentration in soil in the presence of PTE. Vanadium appeared to be the best option for the degradation of diazinon. The models predicted the performance of V²⁺ for diazinon degradation with R² and RMSE of 0.99 and 2.18 <span>(mg.kg^{-1})</span> for SVR and, 0.99, and 1.30 for the ANFIS model for the training set. Finally, the high accuracy of the models was confirmed.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous devulcanization and denitrification: a novel approach for valorization of both ground tire rubber and nitrate-containing wastewater","authors":"Tercan Çataklı,&nbsp;Tuba Hande Erguder","doi":"10.1007/s10532-024-10105-1","DOIUrl":"10.1007/s10532-024-10105-1","url":null,"abstract":"<div><p>This study focused on a new approach for valorization of both ground tire rubber (GTR) and nitrate-containing wastewater via simultaneous devulcanization and denitrification. Initially, sulfur-based autotrophic denitrifiers were successfully enriched from three different seed sludge sources, biological nutrient removal (BNR) sludge, anaerobic digester sludge and BNR sludge of a leather organized industrial zone WWTP. Average nitrate removal efficiencies were 96–98%. Biological devulcanization (biodevulcanization) of GTR was later investigated with these enriched cultures. Results revealed that biodevulcanization was only achieved with the culture enriched from BNR sludge of the leather organized industrial zone WWTP, as 3.9% sulfur removal (desulfurization efficiency). Metal sulfate precipitation was speculated to cause an underestimation of the desulfurization ratio. Fourier-transform infrared spectroscopy (FTIR) results demonstrated a decrease in the intensity of the C–S bonds and an increase in intensity of S–O bonds in treated GTR samples. This was attributed to the oxidation of sulfidic crosslinks, i.e. verification of biodevulcanization. This study indicated that simultaneous biodevulcanization and denitrification could be a promising process for valorization of both GTR and nitrate-containing wastewater which in turn would support circular economy and sustainable development.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity of dishwashers in different cities of China in spring, summer, autumn and winter","authors":"Shuai Yuan,&nbsp;Su Yao,&nbsp;Lingling Pang,&nbsp;Hua Xu,&nbsp;Feirong Bai,&nbsp;Jiali Kang","doi":"10.1007/s10532-024-10098-x","DOIUrl":"10.1007/s10532-024-10098-x","url":null,"abstract":"<div><p>More and more Chinese families are using dishwashers, consumers have paid special attention to the sterilization and disinfection function of dishwashers in recent years. However, there is still a lack of research on the distribution of microorganisms in dishwashers nationwide in China. In order to better upgrade the sterilization and disinfection functions of dishwashers, the plate culture method and high-throughput sequencing technology were used to comprehensively analyze the microbiology of household dishwashers in different cities of China in spring, summer, autumn and winter in this study. A total of 1109 strains of bacteria were isolated from dishwashing machine samples by culturable method, including 706 strains of bacteria distributed in 72 genera, 403 strains of fungi distributed in 52 genera.The most frequently isolated bacteria were <i>Bacillus</i>, <i>Pseudomonas</i>, <i>Brevibacillus</i>, <i>Exiguobacterium</i>, and <i>Acinetobacter</i>. The most frequently isolated fungi were <i>Aspergillus</i>, <i>Penicillium</i>, <i>Exophiala</i>, <i>Fusarium,</i> and <i>Candida</i>. A total of 3779 OTUs were obtained from bacteria and 1541 OTUs were obtained from fungi by amplicon sequencing. The results of culture-independent analysis were consistent with those of culturable analysis. This study laid a foundation for the directional screening of superior microbial resources in dishwashers. It provided data support for the further upgrading of the sterilization and disinfection function of the dishwasher.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of microbial community dynamics and chlorinated solvent biodegradation in methane-amended microcosms from an acidic aquifer","authors":"Myung Hwangbo,&nbsp;Rachael Rezes,&nbsp;Kung-Hui Chu,&nbsp;Paul B. Hatzinger","doi":"10.1007/s10532-024-10103-3","DOIUrl":"10.1007/s10532-024-10103-3","url":null,"abstract":"<div><p>Anaerobic bioremediation is rarely an effective strategy to treat chlorinated ethenes such as trichloroethene (TCE) in acidic aquifers because partial dechlorination typically results in accumulation of daughter products. Methanotrophs have the capability of oxidizing TCE and other chlorinated volatile organic compounds (CVOCs) to non-toxic products, but their occurrence, diversity, and biodegradation capabilities in acidic environments are largely unknown. This study investigated the impacts of different methane (CH₄) concentrations and the presence of CVOCs on the community of acidophilic methanotrophs in microcosms prepared from acidic aquifer samples collected upgradient and downgradient of a mulch barrier installed to promote in-situ anaerobic CVOC biodegradation in Maryland, USA. The ability of indigenous methanotrophs to biodegrade CVOCs was also evaluated. Results of stable isotope probing (SIP) and Next Generation Sequencing (NGS) showed that the microbial communities in the microcosms varied by location and were affected by both CH₄ concentration and the presence of different CVOCs, many of which were biodegraded by the indigenous methanotrophs. Data indicate the likelihood of aerobic cometabolic degradation of CVOCs downgradient of the mulch barrier designed for anaerobic treatment. The study extends the overall knowledge of acidophilic methanotrophs in groundwater and shows that these bacteria have significant potential for degrading CVOCs even at low CH₄ concentrations.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disentangling the microbial genomic traits associated with aromatic hydrocarbon degradation in a jet fuel-contaminated aquifer","authors":"K. J. Hidalgo,&nbsp;V. B. Centurion,&nbsp;L. N. Lemos,&nbsp;A. U. Soriano,&nbsp;E. Valoni,&nbsp;M. P. Baessa,&nbsp;H. H. Richnow,&nbsp;C. Vogt,&nbsp;V. M. Oliveira","doi":"10.1007/s10532-024-10100-6","DOIUrl":"10.1007/s10532-024-10100-6","url":null,"abstract":"<div><p>Spills of petroleum or its derivatives in the environment lead to an enrichment of microorganisms able to degrade such compounds. The interactions taking place in such microbial communities are complex and poorly understood, since they depend on multiple factors, including diversity and metabolic potential of the microorganisms and a broad range of fluctuating environmental conditions. In our previous study, a complete characterization, based on high-throughput sequencing, was performed in a jet-fuel plume using soil samples and in in-situ microcosms amended with hydrocarbons and exposed for 120 days. Herein, we propose a metabolic model to describe the monoaromatic hydrocarbon degradation process that takes place in such jet-fuel-contaminated sites, by combining genome-centered analysis, functional predictions, and flux balance analysis (FBA). In total, twenty high/medium quality MAGs were recovered; three of them assigned to anaerobic bacteria (Thermincolales, <i>Geobacter</i> and Pelotomaculaceace) and one affiliated to the aerobic bacterium <i>Acinetobacter radioresistens</i>, potentially the main players of hydrocarbon degradation in jet-fuel plumes. Taxonomic assignment of the genes indicated that a putative new species of Geobacteria has the potential for anaerobic degradation pathway, while the Pelotomaculaceae and Thermincolales members probably act via syntrophy oxidizing acetate and hydrogen (fermentation products of oil degradation) via sulfate and/or nitrate reduction.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing dairy waste: emerging solutions in conjunction with microbial engineering","authors":"Rishi Bhatia,&nbsp;Shambhavi Singh,&nbsp;Vikram Kumar,&nbsp;Neetu K. Taneja,&nbsp;Harinder Singh Oberoi,&nbsp;Komal Chauhan","doi":"10.1007/s10532-024-10104-2","DOIUrl":"10.1007/s10532-024-10104-2","url":null,"abstract":"<div><p>The dairy industry is grappling with significant challenges in managing effluent due to environmental concerns and stringent regulatory demands, necessitating innovative solutions. The paper investigates how microbial engineering is transforming the treatment of dairy wastewater, offering advanced methods to minimize environmental impact and enhance sustainability. It delves into the current challenges faced by the dairy industry, such as regulatory compliance and the limitations of traditional treatment technologies, and introduces microbial engineering as a promising solution for effluent management. Microbial engineering leverages genetic engineering techniques and microorganisms to enhance the efficiency of treatment processes like bioaugmentation and bioremediation. The environmental and economic benefits of microbial engineering, highlighting its potential to reduce pollution and lower operational costs for the dairy industry. The specific figures can vary based on factors like farm size and location, studies suggest that microbial engineering can reduce wastewater pollution by up to 50% and nutrient runoff by 30%. It also identifies key challenges and there are still areas including strains for specific pollutants (drugs, hormones), enhance degradation pathways, and increase microbes’ stability (stress tolerance, long-term viability) that require further innovation to maximize its benefits. Through case studies and success stories, the paper demonstrates practical applications of microbial engineering in managing dairy effluent, illustrating how it can revolutionize industrial practices for a more sustainable future.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}