Biodegradation最新文献

{"title":"Low-pH sulfate reduction in acid mine drainage treatment systems: implications for acidophilic and acid-tolerant sulfate-reducing bacteria - a systematic review.","authors":"Aimae Jean B Lunsayan, Carlito Baltazar Tabelin, Ana Laura Santos, Theerayut Phengsaart, Takahiko Arima, Tetsuo Yasutaka, Hiroshi Habe, Iván Ňancucheo, Carmen Falagán, Mark Anthony I Jose, Mylah Villacorte-Tabelin","doi":"10.1007/s10532-026-10358-y","DOIUrl":"https://doi.org/10.1007/s10532-026-10358-y","url":null,"abstract":"<p><p>Acid mine drainage (AMD) is characterized by persistent acidity, high sulfate and dissolved metal concentrations. Sulfate-reducing bacteria (SRB) are attractive candidates for AMD remediation because dissimilatory sulfate reduction generates alkalinity while producing sulfide that can facilitate metal removal through precipitation. Extending these processes to acidic conditions has increased interest in acidophilic and acid-tolerant SRB (aSRB and atSRB), yet evidence from cultivation, molecular surveys and treatment systems has often been interpreted separately. This systematic review synthesized 53 culture-dependent, culture-independent, and treatment system studies from 2014 to 2024 to examine relationships among taxonomic occurrence, physiological capability, demonstrated low-pH sulfate reduction and treatment performance. Phylogenetic analysis showed that low-pH sulfate-reducing phenotypes were distributed across multiple lineages and 16S rRNA relatedness alone did not predict acid tolerance. Desulfosporosinus was the most consistently represented genus across studies, although its recurrence was influenced by cultivation strategies. Sulfate reduction was demonstrated below pH 3, with sustained low-pH activity most strongly supported by controlled reactor studies; approximately pH 4.0-5.5 emerged as a comparatively well-supported range, while activity at lower pH was more dependent on microbial physiology and experimental conditions. Low-pH sulfate reduction also emerged as a community-level process shaped by electron-donor use, metabolite turnover and complementary microbial functions, while treatment performance additionally depended on biomass retention, hydraulic conditions and sulfide management. The reviewed studies support a distinction between taxonomic presence, demonstrated activity and treatment contribution. Future work should prioritize standardized reporting of active sulfate-reduction conditions, stronger taxon-function validation and long-term field testing of low-pH sulfidogenic systems.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional metabolite signatures reveal biochemical recovery trajectories in oil-polluted soils undergoing bioremediation.","authors":"Laura N Nwogu-Chigozie, Chioma B Chikere, Victor Ezebuiro, Fidele Tugizimana, Musiwalo S Mulaudzi","doi":"10.1007/s10532-026-10355-1","DOIUrl":"https://doi.org/10.1007/s10532-026-10355-1","url":null,"abstract":"<p><p>Reliable candidate indicators of recovery following petroleum contamination remain limited, as remediation success is commonly assessed using hydrocarbon removal alone rather than restoration of soil characteristics. This study integrated physicochemical profiling with untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to resolve biochemical recovery trajectories across polluted soil (PS), soil undergoing bioremediation (UB), bioremediated soil (BS), and uncontaminated reference soil (CNTR) in Ogoniland, Nigeria. Total petroleum hydrocarbon (TPH) concentrations decreased progressively from 78.26 ± 6.04 mg/kg in PS to 41.23 ± 1.40 mg/kg in UB and 23.38 ± 1.81 mg/kg in BS, corresponding to an overall 70.1% reduction in BS relative to the PS. Sulphur decreased from 6.36 ± 0.70 wt% to 0.152 ± 0.052 wt%, whereas phosphorus increased from 0.068 ± 0.006 wt% to 0.325 ± 0.025 wt% after remediation. Principal component analysis of physicochemical variables explained 99.3% of total variance and resolved a directional restoration gradient (PS → UB → BS → CNTR). Untargeted metabolomics similarly separated treatment states, with PCA explaining 73.7% of total biochemical variance. Polluted and transitional soils were enriched in aromatic stress metabolites, including fluorene, triphenylphosphine oxide, quinolinic acid, and 2,4-dinitrophenol, whereas remediated soils accumulated recovery-associated metabolites such as citric acid, aconitic acid, ectoine, pyridoxamine, lipoic acid, and geranic acid. Integrated analyses showed strong positive correlations between TPH and fluorene (r = 0.88) and Triphenylphosphine oxide (TPPO) (r = 0.84), but negative correlations with citric acid (r = -0.82) and pyridoxamine (r = -0.78). These results demonstrate that hydrocarbon attenuation is tightly coupled to coordinated metabolic reprogramming and nutrient restoration. Functional metabolite signatures may provide a framework for the assessment of biochemical changes associated with soil recovery following petroleum contamination. Thus, our study demonstrates coordinated physicochemical and metabolomic shifts associated with soil recovery during bioremediation.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of chloride, sulphate, phosphate, and silicate-based potassium fertilizers and rice husk biochar on cadmium fate, complexation, leaching and uptake in maize under alkaline soil conditions.","authors":"Muhammad Ashar Ayub, Muhammad Zia Ur Rehman, Mujahid Ali, Muhammad Younas, Hesham F Alharby, Ali Majrashi, Amnah M Alamri, Amal A M Al-Ghamdi","doi":"10.1007/s10532-026-10364-0","DOIUrl":"https://doi.org/10.1007/s10532-026-10364-0","url":null,"abstract":"<p><p>Cadmium (Cd) behavior in alkaline soils is primarily controlled by the interplay of solution complexation, anion- mediated transformation, and solid-phase interactions. The experiment included a series of integrated analyses to monitor Cd speciation, mobility, transformation, leaching, and fate in maize plants. Soil solution chemistry, bioavailability, and leachate-mediated Cd release kinetics were monitored alongside maize growth, yield, and Cd accumulation patterns, as well as associated health risks. In the present work, we examined Cd fate under five potassium-sourced (@200 mg kg^-1 K) fertilizers (K-0-Control, KCl, K₂SO₄, K₂HPO₄, K₂SiO₃) with and without 1% rice husk biochar (RHB) for the maize crop. The chloride (Cl^-) promoted formation of highly soluble Cd complexes promoting Cd displacement and leaching, in comparison sulphate (SO₄^2-) resulted in less mobile fraction of Cd compared to control with no K. Potassium related phosphate (HPO₄^2-) and silicate (SiO₃^2-) anions resulted in substantial decrease in bioavailable fractions as well as leaching of Cd from soil, along with decreased maize root, shoot and grain accumulations, coupled with higher maize yield, improved physiology, biochemical performances and crop health. The improved maize crop parameters with potassium-sourced nutrition suggest a role for potassium nutrition in the net reversal of Cd toxicity, mutually driven by HPO₄^2- and SiO₃^- anions. The inclusion of RHB resulted in a substantial reduction in Cl^- mediated Cd mobility, along with further enhancing HPO₄^2- and SiO₃^2- mediated Cd immobilization in soil, along with better plant growth and yield, proving it a better supplementary soil amendment.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of hair waste for keratinase production using Bacillus altitudinis VK-1120 and its application in enzymatic dehairing","authors":"Vijan Lal Vikash,&nbsp;Pradeep Srinivasan,&nbsp;Parthasarathy Baskaran Sujiritha,&nbsp;Anandasadagopan Suresh Kumar,&nbsp;Ponesakki Ganesan,&nbsp;Balaraman Madhan,&nbsp;Numbi Ramudu Kamini","doi":"10.1007/s10532-026-10363-1","DOIUrl":"10.1007/s10532-026-10363-1","url":null,"abstract":"<div><p>The leather industry impacts the environment through the use of hazardous chemicals for dehairing and the improper disposal of waste from these processes. In this study, a newly isolated <i>Bacillus altitudinis</i> VK-1120 was utilized for hair waste valorization and keratinase production. The keratinase production was optimized, and the maximum activity of 220.82 U/mL was achieved at 40 °C, pH 8.0, and 100 rpm after 48 h. The activity of purified enzyme was inhibited by PMSF and EDTA, suggesting that the enzyme functions as a serine-metalloprotease. SDS-PAGE results revealed a molecular weight of ~25 kDa for the purified keratinase. The optimum temperature and pH for the purified keratinase were 50 °C and 9.0, respectively. The keratinase solution was applied to the dehairing of goatskins using the industrial drum-based method, and complete dehairing was observed within 5 h. The enzymatic treatment significantly improved the waste liquor quality, reducing Biochemical Oxygen Demand (BOD) by 38.67%, Chemical Oxygen Demand (COD) by 50.16%, Total Dissolved Solids (TDS) by 65.25%, and Total Solids (TS) by 52.22%. The physical and organoleptic properties of enzymatically dehaired leather were comparable to those processed by the conventional lime and sulfide method. Additionally, the recyclability of the dehairing enzyme for up to 3 cycles enhanced the application of the keratinase for industrial-scale depilation. Therefore, this study could pave the way for more environmentally responsible solid waste management and depilation practices in the leather industry.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable activated carbon derived from Cyanthillium cinereum biomass for efficient adsorption of methylene blue and eosin yellow","authors":"K. Shakitha,&nbsp;V. N. Meena Devi","doi":"10.1007/s10532-026-10362-2","DOIUrl":"10.1007/s10532-026-10362-2","url":null,"abstract":"<div><p>Biomass-derived activated carbon is widely recognized as an eco-friendly and sustainable adsorbent for wastewater treatment. In this study, activated carbon was synthesized from the aerial parts of <i>Cyanthillium cinereum</i> through phosphoric acid activation followed by controlled carbonization. The prepared CC–AC was characterized using XRD, FTIR, SEM, EDX, BET, and zeta-potential analyses, confirming its amorphous structure, oxygen-containing surface functionalities, well-developed porous characteristics, and pH-dependent surface charge behavior. The adsorption performance of CC–AC was evaluated for the removal of Methylene Blue and Eosin Yellow under different conditions such as pH, contact time, adsorbent dosage, and initial dye concentration. The results showed high removal efficiencies of 90% for Methylene Blue and 83% for Eosin Yellow. Adsorption kinetics were best described mathematically by the pseudo-second-order model, while intraparticle-diffusion and Boyd analyses indicated the involvement of multiple mass-transfer steps. Equilibrium data were best described by the Langmuir model, indicating predominant Langmuir-type equilibrium behavior. The findings demonstrate that <i>Cyanthillium cinereum</i>-derived activated carbon is a cost-effective and sustainable adsorbent for dye-contaminated wastewater treatment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148837987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the Fenton pretreatment on enhanced biological phosphorus removal: emphasizing variations in microbial community and metabolism","authors":"Yuting Wang,&nbsp;Jinmei Wen,&nbsp;Shuyao Wang,&nbsp;Xi Zhu,&nbsp;Jing Wu,&nbsp;Jing Shi","doi":"10.1007/s10532-026-10361-3","DOIUrl":"10.1007/s10532-026-10361-3","url":null,"abstract":"<div><p>The enhanced biological phosphorus removal (EBPR) process is widely used to remove inorganic phosphorus from wastewater. Phosphorus-accumulating organisms (PAOs) play a central role in this process. However, for wastewater containing organic phosphorus, a pretreatment oxidation step is necessary to convert organic phosphorus into inorganic forms before EBPR. In this study, a combined system consisting of a Schorl/H₂O₂ oxidation unit and an EBPR reactor was established to treat fosfomycin, an organophosphorus antibiotic. After the addition of the Schorl/H₂O₂ oxidation influent, the phosphorus accumulation in the EBPR system decreased significantly from 98.12 to 11.08 mg/L. High-throughput sequencing was employed to investigate the microbial community and functional genes in the EBPR system. The significant inhibition of phosphorus removal was mainly attributed to a shift in the dominant microbial species. Specifically, the abundance of the inorganic phosphate remover <i>Casimicrobium_huifangae</i> dropped from 13.6 to 2.3%. In contrast, the abundance of the organophosphorus degrader <i>Acidovorax_sp._KKS102</i> increased from 0.8 to 14.3%. Correspondingly, the abundance of key functional genes (<i>PPK2, yjcG, PhaC</i>) involved in phosphate accumulation and phosphorus release decreased. Metabolic pathway analysis further indicated that the Schorl/H₂O₂ oxidation effluent markedly enhanced the abundance of peroxisomes, which help mitigate oxidative stress. It also enriched pathways related to phosphonate and phosphinate metabolism. This study provides novel insights into the integrated application of advanced oxidation and EBPR for organic phosphorus removal and offers technical guidance for future process design.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148837988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot-scale thermophilic co-digestion of poultry slaughterhouse waste and the organic fraction of municipal solid waste: process stability and kinetic assessment","authors":"Lamia Kadir,&nbsp;Meryem Saber,&nbsp;Insaf Tou,&nbsp;Aissa Khelifi,&nbsp;Noureddine Yassaa","doi":"10.1007/s10532-026-10357-z","DOIUrl":"10.1007/s10532-026-10357-z","url":null,"abstract":"<div><p>Poultry slaughterhouse residues, rich in organic matter (proteins and fats), represent an excellent substrate for biogas production through anaerobic digestion. This study aimed to evaluate the performance and stability of thermophilic mono-digestion of poultry slaughterhouse waste (PSW) and its co-digestion with the organic fraction of municipal solid waste (OFMSW), and to assess methane production kinetics under pilot-scale conditions. Experiments were performed in a 50 L pilot-scale digester operated at 55 °C for 41 days. Mono-digestion of PSW was conducted at a substrate-to-inoculum (S/I₁) ratio of 1:2 (VS basis), whereas co-digestion of PSW and OFMSW was performed at a substrate-to-inoculum (S/I₂) ratio of 1:3 (VS basis). The mono-digestion of PSW produced 1443 NL kg⁻¹ VS of biogas and 916 NL CH₄ kg⁻¹ VS of biomethane. Co-digestion of PSW and OFMSW generated 1134 NL kg⁻¹ VS of biogas and 649 NL CH₄ kg⁻¹ VS of biomethane, corresponding to an approximately 25% higher methane yield than OFMSW mono-digestion. This improvement was attributed to the synergistic effect between both substrates, which enhanced substrate biodegradability while maintaining stable thermophilic digestion. Four kinetic models were evaluated, with the Logistic model providing the best fit for PSW (R² &gt; 0.99), the modified Gompertz model for OFMSW (R² &gt; 0.97), and the Richards model for PSW/OFMSW co-digestion (R² &gt; 0.92). Overall, pilot-scale thermophilic co-digestion of PSW and OFMSW proved to be a sustainable strategy for enhancing methane recovery while ensuring stable process performance, and kinetic modeling provided valuable insight into substrate biodegradation behavior.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced toluene biodegradation by novel bioactive coatings applied in continuous flow bioreactors","authors":"Javier González-Martín,&nbsp;Raquel Lebrero,&nbsp;Raúl Muñoz","doi":"10.1007/s10532-026-10351-5","DOIUrl":"10.1007/s10532-026-10351-5","url":null,"abstract":"<div><p>Volatile organic compounds (VOCs) are common air pollutants from industrial sources. The hydrophobic nature of some of these VOCs limits their removal by conventional biotechnologies. This study evaluates the application of bioactive coatings, a novel biotechnology based on nanoporous polymer matrices embedding active microorganisms, to improve the bioavailability of hydrophobic VOCs in gas-phase bioreactors. Bioactive coatings supported on jute rope and dishcloth strips were tested in a bioreactor for the continuous treatment of toluene and then compared with a conventional biofilm-based system. Jute rope failed to ensure an effective nutrient transport due to the lack of capillarity, while dishcloth strips successfully supported the bioactive coating, significantly enhancing bioreactor’s performance. The bioactive coating bioreactor packed with dishcloth strips outperformed the conventional reactor during the first 45 days, achieving steady-state toluene removals of 77% compared to 67%, with peak efficiency at 89%. Toluene biodegradation in both systems was highly dependent on nitrogen availability. From day 45, the conventional bioreactor improved, reaching steady-state removals over 87%. Furthermore, the addition of silicone oil to the bioactive coating-based bioreactor on day 70 increased toluene removal by a factor of 1.23. These findings demonstrate the potential of bioactive coatings and capillary supports to enhance VOC removal in bioreactors.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148782663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial threats to aviation fuels: susceptibility and biostability studies","authors":"Evgeny A. Sharin,&nbsp;Valeriy A. Karpov,&nbsp;Tatiana A. Semenova,&nbsp;Oxana A. Matveeva,&nbsp;Vadym G. Krylov,&nbsp;Uliana A. Makhova,&nbsp;Andrei V. Ilin,&nbsp;Nikita A. Klimov,&nbsp;Marina M. Lobashova,&nbsp;Vladimir M. Kapustin,&nbsp;Mikhail A. Ershov","doi":"10.1007/s10532-026-10340-8","DOIUrl":"10.1007/s10532-026-10340-8","url":null,"abstract":"<div><p>Biodegradation of aviation fuels remains a critical factor during storage and operation, affecting both the composition and performance properties of kerosene fractions. This study aims to establish the relationship between the component composition of modern jet fuels and their biostability. In addition, the effect of typical additives was evaluated. Four industrially used kerosene fractions differing in refining depth were investigated—straight-run, hydrodemercaptanized, hydrotreated, and hydrocracked—along with their mixtures containing antioxidant (Agidol-1) and lubricity additive (Unicor J). Biological tests were conducted under standardized conditions (IP 385/ASTM D6974; GOST 9.023) using <i>Amorphotheca resinae</i> as the test organism; changes in group hydrocarbon composition were analyzed by GC × GC–MS. It was found that susceptibility to biodegradation decreases with increasing refining depth, while microbial activity consistently resulted in a reduction in paraffinic and naphthenic hydrocarbons and a relative increase in aromatics; simultaneously, sulfur content decreases. Paradoxically, the most pronounced increases in acidity and existent gum content were observed in the hydrotreated and hydrocracked fractions. Agidol-1 exhibited biostatic (antimicrobial) activity, but was accompanied by a marked drop in aqueous phase pH (≈ 4), suggesting increased corrosion risk associated with the aqueous phase. Unicor J produced a slight positive effect, likely due to its surface-active properties.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 4","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosurfactant–nonionic mixed micelles enhance heavy crude oil bioremediation: interfacial synergy and multilevel microbial responses","authors":"Yang Wang,&nbsp;Ziyue Shang,&nbsp;Fei Wu,&nbsp;Yuhua Su,&nbsp;Liangzhen Du,&nbsp;Hailiang Yin,&nbsp;Qiyou Liu","doi":"10.1007/s10532-026-10354-2","DOIUrl":"10.1007/s10532-026-10354-2","url":null,"abstract":"<div><p>To address the water–oil–cell interfacial mass-transfer barrier limiting microbial degradation of heavy crude oil, this study evaluated a biosurfactant–nonionic mixed-micelle strategy that integrates interfacial performance with microbial compatibility. Four binary surfactant systems were screened using the petroleum hydrocarbon-degrading consortium B10. Among them, the rhamnolipid/Triton X-100 formulation, designated RT, showed the best overall balance of mixed-micelle synergy, crude-oil solubilization, microbial growth compatibility, and apparent hydrocarbon attenuation. After 7 d, RT achieved an apparent attenuation efficiency of 60.0%, compared with 21.9% in the surfactant-free B10 control, with the largest increases observed for the C16–C22 and C23–C32 fractions. RT treatment was accompanied by increased protein-like extracellular polymeric substance (EPS) content, changes in FTIR and fluorescence characteristics, a more negative zeta potential, and greater apparent cell-surface hydrophobicity. Metatranscriptomic analysis further revealed higher transcript abundance of genes associated with motility and chemotaxis, membrane transport, fatty acid metabolism, and the tricarboxylic acid cycle. Together, these multidimensional observations support a proposed mechanism model in which mixed-micelle interfacial regulation is associated with EPS compositional responses, cell-surface property changes, and consortium-level transcriptional adjustments, rather than solubilization alone.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 4","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}