Biodegradation最新文献

筛选
英文 中文
Machine learning-based optimization of biogas and methane yields in UASB reactors for treating domestic wastewater. 基于机器学习的UASB反应器处理生活污水沼气和甲烷产量优化。
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-26 DOI: 10.1007/s10532-025-10152-2
Saurabh Kumar, Saurabh Kumar, Divesh Ranjan Kumar, Dayanand Sharma, Warit Wipulanusat
{"title":"Machine learning-based optimization of biogas and methane yields in UASB reactors for treating domestic wastewater.","authors":"Saurabh Kumar, Saurabh Kumar, Divesh Ranjan Kumar, Dayanand Sharma, Warit Wipulanusat","doi":"10.1007/s10532-025-10152-2","DOIUrl":"https://doi.org/10.1007/s10532-025-10152-2","url":null,"abstract":"<p><p>This study aimed to optimize biogas and methane production from Up-flow anaerobic sludge blanket reactors for treating domestic wastewater using advanced machine learning models-namely, eXtreme Gradient Boosting (XGBoost) and its hybridized form, XGBoost, integrated with particle swarm optimization (XGBoost-PSO). The key operational variables included time, flow rate, chemical oxygen demand (COD), pH, volatile fatty acids, total suspended solids, hydraulic retention time, alkalinity, and the organic loading rate. Empirical data used to train and validate the predictive models were acquired from the sequential treatment of laboratory-prepared low-strength synthetic wastewater and actual municipal wastewater samples. Data was collected from two treatment phases: synthetic wastewater (COD: 335.45 ± 28.32 mg/L) was treated from days 0 to 270, followed by real domestic wastewater (COD: 225.28 ± 65.98 mg/L) from days 0 to 130. Gas production was continuously monitored throughout. The XGBoost-PSO model outperformed the standard XGBoost algorithm in both the training and testing phases. For biogas prediction during training, XGBoost-PSO achieved an RMSE of 0.0405, an MAE of 0.0225, and an R<sup>2</sup> of 0.9832, whereas for methane, the values were an RMSE of 0.0257, an MAE of 0.0175, and an R<sup>2</sup> of 0.9942. The testing results further confirmed the model's robustness, with RMSE, MAE, and R<sup>2</sup> values of 0.1017, 0.0676, and 0.9404 for biogas and 0.0694, 0.0519, and 0.9717 for methane, respectively. These findings highlight the potential of integrating artificial intelligence-driven approaches to optimize bioenergy recovery in wastewater treatment systems.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"55"},"PeriodicalIF":3.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways. barthaii Sphingobium KK22对C9芳烃正丙苯的利用及多种生物降解途径的共存
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-23 DOI: 10.1007/s10532-025-10149-x
Miharu Sakai, Jiro F Mori, Robert A Kanaly
{"title":"Utilization of the C9 aromatic hydrocarbon n-propylbenzene by Sphingobium barthaii KK22 and coexistence of multiple biodegradation pathways.","authors":"Miharu Sakai, Jiro F Mori, Robert A Kanaly","doi":"10.1007/s10532-025-10149-x","DOIUrl":"https://doi.org/10.1007/s10532-025-10149-x","url":null,"abstract":"<p><p>n-Propylbenzene is an environmental pollutant belonging to a class of heavily used nonpolar alkylated aromatic solvents referred to as the C9 aromatics. Although n-propylbenzene is detected in different environmental matrices and displays toxicity, its bacterial biodegradation has been little explored. Consequently, few transformation products have been identified, and comprehensive biodegradation pathways were not constructed. Understanding n-propylbenzene biotransformation shall be useful to predict its fate and transport in the environment. Therefore, n-propylbenzene biotransformation by soil bacterium, Sphingobium barthaii KK22, was examined by liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) through product ion scan collision induced dissociation (CID) analyses. Targeted CID of unknown biotransformation products resulted in the proposal of structures for at least 18 compounds and based upon these results, metabolites were organized into biotransformation pathways which revealed multiple routes to the TCA cycle. Decarboxylation of the n-propylbenzene alkyl side chain was proposed as a key part of the biodegradation process-so-called alkyl chain shortening. At the same time, the aromatic ring of n-propylbenzene was vulnerable to dioxygenation no matter the alkyl chain length or degree of alkyl chain oxidation resulting in numerous 3-, 2- and 1-carbon chain length compounds and their aromatic ring-opened counterparts. Quantitative analyses by LC and growth monitoring by absorbance confirmed that this bacterium eliminated 100 mg/L n-propylbenzene from culture media and that it utilized n-propylbenzene as a carbon source. In the natural environment, catabolically versatile soil sphingomonads such as S. barthaii may be contributors to the biodegradation of alkylated aromatic nonpolar pollutants such as n-propylbenzene.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"54"},"PeriodicalIF":3.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biodegradation of pretilachlor and butachlor by novel bacterial strains isolated from paddy field soil. 稻田土壤中分离的新型细菌降解甲草胺和丁草胺的研究。
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-21 DOI: 10.1007/s10532-025-10148-y
Nguyen Thi Oanh, Ha Danh Duc
{"title":"Biodegradation of pretilachlor and butachlor by novel bacterial strains isolated from paddy field soil.","authors":"Nguyen Thi Oanh, Ha Danh Duc","doi":"10.1007/s10532-025-10148-y","DOIUrl":"10.1007/s10532-025-10148-y","url":null,"abstract":"<p><p>In this study, two pretilachlor-degrading bacterial strains isolated from soil, Enterobacter sp. Pre1 and Pseudomonas sp. Pre2 completely utilized the compound as a sole carbon, energy and nitrogen source under aerobic conditions. The determination of degradation kinetics revealed that the rates of both isolates followed the Michaelis-Menten model, in which the maximum utilization rates of Enterobacter sp. Pre1 and Pseudomonas sp. Pre2 were 0.010 ± 0.0012 and 0.0060 ± 0.0007 mM/h, respectively. Moreover, Pseudomonas sp. Pre2 exhibited effective degradation of butachlor. Enterobacter sp. Pre1 showed better biofilm formation than the later one. Their immobilized biomass in polyurethane foam (PUF) reached 323.4 ± 35.6 mg/g PUF completely degrading pretilachlor at 0.15 mM within 12 h in a packed bed bioreactor. A metabolite, 2,6-diethylaniline, was produced during the degradation by both strains. Besides, 4-amino-3,5-diethyl phenol and aniline were the metabolites in the degradation by Enterobacter sp. Pre1 and Pseudomonas sp. Pre2, respectively. This study confirmed the efficiency and mechanisms in the degradation of pretilachlor by freely suspended and immobilized cells of the isolated bacteria.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"53"},"PeriodicalIF":3.1,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioremediation of Cr(VI) using novel thermophilic bacteria Brevibacillus borstelensis SSAU-3 T: optimization, mechanism and phytotoxicity study. 新型嗜热细菌短芽孢杆菌ssau - 3t对铬(VI)的生物修复:优化、机制及植物毒性研究
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-20 DOI: 10.1007/s10532-025-10145-1
Manshi Agrawal, Abhijeet Sharma, Akanksha Singh, Shanthy Sundaram
{"title":"Bioremediation of Cr(VI) using novel thermophilic bacteria Brevibacillus borstelensis SSAU-3 T: optimization, mechanism and phytotoxicity study.","authors":"Manshi Agrawal, Abhijeet Sharma, Akanksha Singh, Shanthy Sundaram","doi":"10.1007/s10532-025-10145-1","DOIUrl":"10.1007/s10532-025-10145-1","url":null,"abstract":"<p><p>Rapid industrialization and advancement of chemical fertilizers in agriculture get infused with water causing heavy pollution of inorganic pollutants has become a serious problem. This research focuses on the utilization of a thermophilic bacteria Brevibacillus borstelensis, SSAU-3 T in the bioremediation of hexavalent chromium (Cr (VI)). The strain has capability in > 99% removal of 40 ppm Cr (VI). Further optimization was studied by varying parameters (pH, Inoculum size, salinity, volume and temperature) based on Cr(VI) removal capabilities. Removal mechanism was determined by studying thermodynamic, kinetic, and isotherm under optimized parameters: pH 7, salinity 5 g/L, inoculum size 2%, medium volume 20 mL, temperature 55 °C which resulted that Redlich-Peterson isotherm model is a best fit for this study. Characterization of functional groups and bonds present on bacterial cell surface before and after treatment with chromium were optimized by Fourier Transform Infrared Spectroscopy and surface morphology changes were also observed by Scanning Electron Microscopy. A phytotoxicity study was conducted on wheat, which showed that bacterial secondary metabolites were not toxic. The study highlights an eco-friendly and cost-effective approach to mitigate Cr(VI) toxicity using thermophilic microbes from hot springs.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"52"},"PeriodicalIF":3.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mycotransformation of anthracene by indigenous Trichoderma lixii and Talaromyces pinophilus isolates: insights into the metabolic pathways, enzyme profiles and acute toxicity. 原生立希木霉和嗜松木霉菌株对蒽的菌转化:代谢途径、酶谱和急性毒性的见解。
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-18 DOI: 10.1007/s10532-025-10147-z
Samson O Egbewale, Ajit Kumar, Mduduzi P Mokoena, Ademola O Olaniran
{"title":"Mycotransformation of anthracene by indigenous Trichoderma lixii and Talaromyces pinophilus isolates: insights into the metabolic pathways, enzyme profiles and acute toxicity.","authors":"Samson O Egbewale, Ajit Kumar, Mduduzi P Mokoena, Ademola O Olaniran","doi":"10.1007/s10532-025-10147-z","DOIUrl":"10.1007/s10532-025-10147-z","url":null,"abstract":"<p><p>This study focused on the mycotransformation of a very prominent PAH, anthracene, and its acute toxicity reduction by Ascomycete fungi: Trichoderma lixii strain FLU1 (TlFLU1) and Talaromyces pinophilus strain FLU12 (TpFLU12), indigenously isolated from benzo[b] fluoranthene-enriched activated sludge. The results indicate that both the isolates TlFLU1 and TpFLU12 could tolerate anthracene exposure up to 1000 mg/L, with increased expression of ligninolytic enzymes: Laccase, Lignin peroxidase, and Manganese peroxidase. The mycotransformation of anthracene was observed to be growth-linked and mediated by the expression of the intracellular enzymes as the initial mechanism used by these strains followed by the ligninolytic enzymes with up to 56% and 38% anthracene degradation by TlFLU1 and TpFLU12, respectively, after 24 days with a concomitant change in pH from 5 to 4 (TlFLU1) and 6.2 (TpFLU12). The GC-MS and FTIR analysis of the samples indicate the appearance of metabolic intermediates: 9,10 anthracenedione and benzoic acid in TlFLU1 grown medium, while anthrone and 9,10 anthracenedione were detected in TpFLU12 grown medium. The mycotransformation of the compound followed a first-order kinetic model with an effective concentration (EC<sub>50</sub>) of 262.3-266.1 mg/L, with a toxicity unit (TU) of 0.4% in Vibrio parahaemolyticus (6 h exposure) to each intermediate. Results show efficient mycotransformation of anthracene into a non-toxic state by TlFLU1 and TpFLU12.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"51"},"PeriodicalIF":3.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of pH on the biodegradation efficiency of fats, oils, and grease by biosurfactant-producing bacterial consortia. pH值对产生生物表面活性剂的细菌群落对脂肪、油和油脂生物降解效率的影响。
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-16 DOI: 10.1007/s10532-025-10146-0
Jia-Ying Wong, Ngui-Sing Ngieng, Ahmad Husaini, Rosmawati Saat, Hasnain Hussain
{"title":"Influence of pH on the biodegradation efficiency of fats, oils, and grease by biosurfactant-producing bacterial consortia.","authors":"Jia-Ying Wong, Ngui-Sing Ngieng, Ahmad Husaini, Rosmawati Saat, Hasnain Hussain","doi":"10.1007/s10532-025-10146-0","DOIUrl":"10.1007/s10532-025-10146-0","url":null,"abstract":"<p><p>The accumulation of fats, oils, and grease (FOG) in wastewater systems presents major environmental challenges, necessitating the development of effective bioremediation strategies. Biosurfactant-producing bacteria are promising for FOG degradation; however, their efficacy is highly pH-dependent, affecting microbial metabolism and biosurfactant stability. This study evaluates the impact of pH on FOG biodegradation by locally isolated biosurfactant-producing bacterial consortia to identify optimal pH conditions. Two highly efficient biosurfactant-producing bacterial isolates, identified via 16S rRNA sequencing as Pseudomonas aeruginosa and Bacillus velezensis, were cultured in Bushnell Haas (BH) medium to form a bacterial consortium. The consortium was then inoculated into fresh BH medium, adjusted to pH values from 4 to 9, and supplemented with 1% FOG (w/v). Samples were monitored at six-day intervals for 30 days under continuous shaking at 130 rpm. After 30 days of biodegradation, the solid FOGs in pH 6 disappeared while flocs were observed in both pH 4 and 5. Despite greater floc formation at pH 6, GC-MS analysis revealed that pH 4 achieved the highest degradation rate, displaying the fewest FOG peaks and the lowest area under peaks, indicating the most substantial FOG reduction. Notably, the consortium achieved the highest FOG removal at pH 4, an acidic condition under which most long-chain FOG components were completely degraded or transformed into shorter chains. This finding reveals an unexpected optimum pH 4 for FOG bioremediation by two efficient biosurfactant-producing bacteria combined into a synergistic consortium, highlighting a potential strategy to enhance grease waste treatment.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"50"},"PeriodicalIF":3.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pesticide degradation capacity of a novel strain belonging to Serratia sarumanii with its genomic profile. 萨鲁曼沙雷氏菌新菌株的农药降解能力及其基因组图谱。
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-06-01 DOI: 10.1007/s10532-025-10144-2
Gülperi Alatassi, Ömür Baysal, Ragıp Soner Silme, Gülçin Pınar Örnek, Hakan Örnek, Ahmet Can
{"title":"Pesticide degradation capacity of a novel strain belonging to Serratia sarumanii with its genomic profile.","authors":"Gülperi Alatassi, Ömür Baysal, Ragıp Soner Silme, Gülçin Pınar Örnek, Hakan Örnek, Ahmet Can","doi":"10.1007/s10532-025-10144-2","DOIUrl":"10.1007/s10532-025-10144-2","url":null,"abstract":"<p><p>The extensive use of pesticides in agricultural practices, coupled with the potential for microbial biodegradation of these chemicals, plays a critical role in environmental sustainability. This study aimed to identify microorganisms capable of degrading the most commonly used pesticides in agricultural fields within our region. In vitro screening revealed a microorganism with a broad pesticide degradation spectrum, and whole-genome sequencing further indicated the presence of genomic regions associated with pesticide degradation, a finding that was validated by LC-MS/MS analysis. Detailed genomic analysis, including ribosomal multi-locus sequence typing (rMLST), identified the microorganism as Serratia sarumanii. Our results also demonstrated that the introduction of this strain into the environment not only promoted the degradation of specific pesticides but also enhanced the efficacy of certain other pesticides at low concentrations through a synergistic interaction. To further substantiate the biodegradation capabilities of the strain, LC-MS/MS chromatographic analysis of 25 pesticide-active chemicals confirmed that Serratia sarumanii effectively biodegrades several pesticide active ingredients, including fludioxonil, fenhexamid, pyrimethanil, and spirodiclofen. These findings underscore the biodegradative potential of Serratia sarumanii and its promising application in the bioremediation of pesticide-contaminated soils.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 3","pages":"49"},"PeriodicalIF":3.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green treatments for polyaromatic hydrocarbons in e-wastes 电子垃圾中多芳烃的绿色处理
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-05-19 DOI: 10.1007/s10532-025-10140-6
Bandita Dutta, Debarati Chatterjee, Arina Guha, Rina Rani Ray
{"title":"Green treatments for polyaromatic hydrocarbons in e-wastes","authors":"Bandita Dutta,&nbsp;Debarati Chatterjee,&nbsp;Arina Guha,&nbsp;Rina Rani Ray","doi":"10.1007/s10532-025-10140-6","DOIUrl":"10.1007/s10532-025-10140-6","url":null,"abstract":"<div><p>Rapid elevation of global population along with increased urbanization and industrialization afflict the water resources leading to the blooming of wastewater. Two or more aromatic rings fused with organic compound Polycyclic Aromatic Hydrocarbons (PAHs) emerged worldwide through anthropogenic processes, mainly due to the incomplete combustion of organic fuels. In accordance with the United States Environmental Protection Agency (USEPA), there are 16 PAHs that are deemed as primary pollutants. These are toxic to the living organisms due to their pervasive existence, rebelliousness, potential for bioaccumulation and carcinogenic venture. Several methods including fixation, incineration and oxidation are put forward to remove PAHs. Occasionally some fictional toxic products are produced by the incomplete removal of PAHs. Bioremediation is one of the ecological techniques to remove the PAHs. Microbial biodegradation is considered as an effective and inexpensive technique to remove PAHs along with other hydrocarbons and xenobiotic compounds and are accomplished by few PAHs degrading bacteria including <i>Haemophilus</i> spp., <i>Mycobacterium</i> spp., <i>Paenibacillus</i> spp., <i>Pseudomonas aeruginosa</i>, <i>P. fluorescens</i>, <i>Rhodococcus</i> spp. along with few biosurfactant-producing microbes. The novel biochemical events involved in hydrocarbon catabolism are microbial physical adaptation, their acquisition and uptake. The bioremediation efficacy can be further ameliorated through genetic modification of the microbes. This chapter will focus on the eco-friendly treatment for the PAHs remediation in in situ and ex situ. This chapter will explore the remediation of the PAH by-products through the multi-process conjunctional treatment processes under the green therapy.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review of enhancement of chlorophenol bioremediation using synergistic effects between zero-valent iron and microorganisms 零价铁与微生物协同增强氯酚生物修复的研究进展
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-05-19 DOI: 10.1007/s10532-025-10133-5
Hao Liu, Deli Wu, Weishi Wang
{"title":"A review of enhancement of chlorophenol bioremediation using synergistic effects between zero-valent iron and microorganisms","authors":"Hao Liu,&nbsp;Deli Wu,&nbsp;Weishi Wang","doi":"10.1007/s10532-025-10133-5","DOIUrl":"10.1007/s10532-025-10133-5","url":null,"abstract":"<div><p>Chlorophenols (CPs) are a class of synthetic organic chemicals that are widely distributed in soil and groundwater, posing significant risks to human health and the environment due to persistence, acute toxicity, and potential carcinogenicity. Zero-valent iron (ZVI) has emerged as a promising remediation technique for CPs, but its efficacy is often hindered by surface passivation, non-target competition, and limited mobility in the subsurface. While CPs are inherently biodegradable, their high toxicity and the lack of functional enzymes in indigenous microbial systems restrict the effectiveness of bioremediation. Recently, a hybrid system integrating ZVI with microbial degradation draws increasingly research interests, paving out a new path for sustainable degradation of CPs. These systems leverage the synergistic interactions between ZVI and microorganisms to enhance CP biodegradation. This review provides a comprehensive analysis of the advancement. Key topics include the enhancement of electron transfer, alterations to microbial communities, mitigation of toxicity, and the interplay between other processes. Operation modes, ZVI dosage, and interactions with naturally occurring iron minerals, are also discussed in the context of applications in soil and groundwater remediation. Despite research efforts and successful implementations, critical knowledge gaps remain, particularly in regard to the characterization of microbial processes in natural systems, highlighting the need for future research.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzymatic degradation of PET by hydrolase from Brucella intermedia IITR130 and its genomic insights 布鲁氏菌中间体IITR130水解酶降解PET及其基因组意义
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-05-17 DOI: 10.1007/s10532-025-10141-5
Pallavi Srivastava, Saurabh Singh, Mohini Soni, J. Venkatesh Pratap, Srikrishna Subramanian, Natesan Manickam
{"title":"Enzymatic degradation of PET by hydrolase from Brucella intermedia IITR130 and its genomic insights","authors":"Pallavi Srivastava,&nbsp;Saurabh Singh,&nbsp;Mohini Soni,&nbsp;J. Venkatesh Pratap,&nbsp;Srikrishna Subramanian,&nbsp;Natesan Manickam","doi":"10.1007/s10532-025-10141-5","DOIUrl":"10.1007/s10532-025-10141-5","url":null,"abstract":"<div><p>Plastic pollution, particularly from polyethylene terephthalate (PET), has become a significant environmental concern, necessitating innovative and sustainable degradation strategies. The present study provides valuable perspectives on the genomic and functional characteristics of <i>Brucella intermedia</i> IITR130, a bacterium capable of degrading PET. Hybrid genome sequencing of IITR130 resulted in identification of two chromosomes combining 4.59 Mbp size. Genomic annotation revealed occurrence of key enzymes involved in the PET sheet biodegradation pathway, including hydrolases, ring hydroxylating dioxygenases, protocatechuate 3,4 dioxygenases, genes for metabolism of several other natural and synthetic plastic. A hydrolase gene Hy1 of 24 kDa, was identified, expressed, and characterized, demonstrating an optimal catalytic activity at 37 °C and pH 8.5. Scanning electron microscopy (SEM) and fourier-transform infrared spectroscopy (FTIR) confirmed substantial degradation of PET surfaces treated with Hy1 protein, resulted in surface erosion, crack formation, and functional group modifications in the range 2150–2550 cm⁻<sup>1</sup> and 2950–3350 cm⁻<sup>1</sup> suggestive of O=C=O stretching and O–H stretching respectively. Monomethyl terephthalate (MMT) and terephthalic acid (TPA) were identified as PET degradation metabolites formed by strain IITR130. Fluorescence quenching showed higher substrate affinity for bis(2-hydroxyethyl) terephthalate (BHET) (K<sub>d</sub> = 148.2) than terephthalic acid (TPA) (K<sub>d</sub> = 674). Moreover, phylogenetic analysis of Hy1 protein revealed that Hy1 containing conserved catalytic triad (Ser<sup>108</sup>, His<sup>188</sup>, Asp<sup>155</sup>) belonging to the family III of hydrolase enzyme sharing a clade with PET degrading hydrolase PETase from <i>Ideonella sakaiensis.</i> These results demonstrate the potential of <i>B. intermedia</i> IITR130 as an efficient biocatalyst for PET biodegradation which could be exploited appropriately for plastic waste management.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信