Biodegradation最新文献

Statistical optimization of crude oil bioremediation using Streptomyces aurantiogriseus isolated from Egypt's Western Desert. 埃及西部沙漠金灰链霉菌生物修复原油的统计优化。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-25 DOI: 10.1007/s10532-025-10154-0

Sahar Y Ibrahim, Eman A Abdelhamid, Ali M El-Hagrassi, Noha M Kamal

{"title":"Statistical optimization of crude oil bioremediation using Streptomyces aurantiogriseus isolated from Egypt's Western Desert.","authors":"Sahar Y Ibrahim, Eman A Abdelhamid, Ali M El-Hagrassi, Noha M Kamal","doi":"10.1007/s10532-025-10154-0","DOIUrl":"https://doi.org/10.1007/s10532-025-10154-0","url":null,"abstract":"One of the most common soil pollutants on a global scale is fuel, which is fundamental for daily activities. Biodegradation has been regarded as an ideal remediation technique for hydrocarbon pollution. We investigated the potential of 28 Streptomyces species inhabiting different hydrocarbon-polluted soils for the biodegradation of petroleum. The tested isolates were cultured on mineral salts broth containing 2% crude oil as the sole carbon source. Gravimetric analysis of residual crude oil was performed, and the samples that showed the highest percentage of biodegradation were also analyzed via gas chromatography. Among the isolated actinobacteria, Streptomyces aurantiogriseus strain NORA7 (EMCC 28565) stood out for its ability to degrade crude oil (66.28 ± 6.25%). Gas chromatography revealed that docosane, nonadecane, pentacosane, and 7-methylpentadecane were the major compounds detected in the residual treated crude oil. Plackett-Burman design (PB) was used to determine the critical factors impacting the biodegradation process. Response surface methodology (RSM) through Central Composite Design (CCD) was subsequently conducted, and the predicted optimum point of crude oil biodegradation was at 3% crude oil concentration, 0.15 g/L yeast extract, and 25 mm inoculum size. The experimental value after optimum conditions was 70% after 3 weeks, which was close to the predicted value. A pot experiment was performed to investigate the outcomes of ex situ soil bioremediation, and the results were consistent with those of the flask-scale biodegradation experiment with enhanced removal of crude oil (92%). The results revealed that the ability of S. aurantiogriseus NORA7 to biodegrade crude oil could significantly contribute to the eco-friendly recovery of oil-polluted ecosystems and reduce the long-term environmental impact of crude oil pollution.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"68"},"PeriodicalIF":3.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of Laccase from Lentinus berteroi and applications for biodegradation of organic micropollutants. 香菇漆酶的制备、表征及其在有机微污染物生物降解中的应用。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-22 DOI: 10.1007/s10532-025-10166-w

Katielle Vieira Avelino, Marisangela Isabel Wietzikoski Halabura, Edileusa Cristina Marques Gerhardt, Maira Gabriela Paetzold, Asif Khan, Juliana Silveira do Valle, Flavio Augusto Vicente Seixas

{"title":"Production and characterization of Laccase from Lentinus berteroi and applications for biodegradation of organic micropollutants.","authors":"Katielle Vieira Avelino, Marisangela Isabel Wietzikoski Halabura, Edileusa Cristina Marques Gerhardt, Maira Gabriela Paetzold, Asif Khan, Juliana Silveira do Valle, Flavio Augusto Vicente Seixas","doi":"10.1007/s10532-025-10166-w","DOIUrl":"https://doi.org/10.1007/s10532-025-10166-w","url":null,"abstract":"This study aimed to produce, purify, and characterize laccase from Lentinus berteroi U21-2, for its potential application in the biodegradation of micropollutants. The fungus was cultivated in a liquid medium for 15 days at 28 °C in the dark. The enzymatic extract was purified using chromatographic techniques, and its molecular mass was determined through gel filtration, SDS-PAGE, and peptide mass fingerprint analysis. The optimum pH, temperature, and residual activity were evaluated using ABTS, guaiacol, and syringaldazine as substrates. UV-Vis spectroscopy and LC-MS/MS techniques were used to assess the laccase biodegradation of micropollutants. The laccase was 3.2-fold purified, and gel filtration estimated its molecular mass at 79.7 kDa, while SDS-PAGE revealed two bands of approximately 66 kDa and 50 kDa, being the ~50 kDa band a fragment. The purified enzyme demonstrated maximum activity at pH 4.5 and 30 °C, retaining 80% of its activity for 58 hours, and a higher affinity for syringaldazine as indicated by the KM. The purified laccase effectively reduced the initial concentration of β-estradiol by 82%, diclofenac by 70%, and bisphenol-A by 30%, but showed no degradation of ibuprofen. These findings highlight the potential of L. berteroi laccase as a promising biocatalyst for the biodegradation of micropollutants.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"64"},"PeriodicalIF":3.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-function strategy: simultaneous biopolymer production and lead biosorption by Bacillus paramycoides in single-batch fermentation process. 双功能策略：在单批发酵过程中，副芽孢杆菌同时生产生物聚合物和铅的生物吸附。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-22 DOI: 10.1007/s10532-025-10160-2

Raghavendra Paduvari, Roopashri Arekal, Divyashree Mysore Somashekara

{"title":"Dual-function strategy: simultaneous biopolymer production and lead biosorption by Bacillus paramycoides in single-batch fermentation process.","authors":"Raghavendra Paduvari, Roopashri Arekal, Divyashree Mysore Somashekara","doi":"10.1007/s10532-025-10160-2","DOIUrl":"https://doi.org/10.1007/s10532-025-10160-2","url":null,"abstract":"The conventional physicochemical processes of lead removal from contaminated wastewater are cost-intensive and generate toxic secondary wastes. Therefore, employing bacteria capable of lead biosorption will serve as a green, sustainable, safe and inexpensive process for lead removal, and its integration with polyhydroxyalkanoate (PHA) production will generate revenue. To integrate these two processes, Bacillus paramycoides isolated from the lake water were analyzed for lead biosorption and PHA production in mineral salt media containing lead nitrate at different incubation times. The bacterium showed a high lead biosorption of about 423 mg/g of biomass at 72 h of incubation, which was earlier not reported. Bacillus paramycoides produced polyhydroxybutyrate by utilizing sucrose in the media. The lead ions enhanced the PHA production up to 35.7% CDW in bacterial cells by inducing oxidative stress, and the ability of PHA polymer to adsorb lead ions prevented further oxidative damage to the cells. This study is the first report on using an integrated process approach for lead biosorption and PHA production in bacteria.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"65"},"PeriodicalIF":3.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterotrophic nitrification-aerobic denitrification characteristics and zinc-containing wastewater treatment potential of pseudomonas hunanensis SK-4: screening, application, and mechanistic insights. 湖南假单胞菌SK-4异养硝化-好氧反硝化特性及含锌废水处理潜力：筛选、应用及机理研究

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-22 DOI: 10.1007/s10532-025-10162-0

Yuhong Zhang, Jiacheng Cai, Jiabao Wang, Yueqing Jiang

{"title":"Heterotrophic nitrification-aerobic denitrification characteristics and zinc-containing wastewater treatment potential of pseudomonas hunanensis SK-4: screening, application, and mechanistic insights.","authors":"Yuhong Zhang, Jiacheng Cai, Jiabao Wang, Yueqing Jiang","doi":"10.1007/s10532-025-10162-0","DOIUrl":"https://doi.org/10.1007/s10532-025-10162-0","url":null,"abstract":"To alleviate the detrimental impacts of zinc-containing wastewater on biological nitrogen removal systems, Pseudomonas hunanensis SK-4 was isolated and screened from a heavy industrial wastewater treatment facility. The efficient heterotrophic nitrification aerobic denitrification ability of Pseudomonas hunanensis was confirmed for the first time. The maximum removal efficiencies achieved by strain SK-4 for ammonium (100 mg/L), nitrate (100 mg/L), and nitrite (50 mg/L) were 99.81%, 82.31%, and 96.10%, respectively. Under the conditions of C/N 10, 35 °C, pH 7, 140 rpm, inoculum size 3%, with sodium citrate utilized as the carbon source, NH₄⁺-N was effectively removed. The nitrogen removal mechanisms employed by strain SK-4 were revealed through detailed whole-genome sequencing analysis. The nitrification and denitrification efficiencies of strain SK-4 were not affected under 100 mg/L Zn(II) stress. The inhibitory effects of varying Zn(II) concentrations on the nitrogen removal efficiency of strain SK-4 were predicted through model fitting. Such results showed that strain SK-4 had strong tolerance to Zn(II). The complete metabolic mechanisms of tolerance, transport, regulation and detoxification of Zn(II) in strain SK-4 were elucidated. Such findings indicated that strain SK-4 has a strong potential for the treatment of Zn(II) and nitrogen composite pollutants.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"66"},"PeriodicalIF":3.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of short-chain phthalate esters (PAEs) by Sinomonas sp. S2 isolated from a soil contaminated by metal(loid)s smelting. 金属熔炼污染土壤中单胞菌S2降解短链邻苯二甲酸酯（PAEs）的研究

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-22 DOI: 10.1007/s10532-025-10159-9

Pengfei Shao, Jun Yao, Miaomiao Li, Ning Min

{"title":"Biodegradation of short-chain phthalate esters (PAEs) by Sinomonas sp. S2 isolated from a soil contaminated by metal(loid)s smelting.","authors":"Pengfei Shao, Jun Yao, Miaomiao Li, Ning Min","doi":"10.1007/s10532-025-10159-9","DOIUrl":"https://doi.org/10.1007/s10532-025-10159-9","url":null,"abstract":"Phthalate esters (PAEs) are widely used as plasticizers and beneficiation flotation agents in ore smelters, which are ubiquitously distributed emerging contaminants in the environment. The biodegradation of PAEs by degrading microbes is a promising method for their remediation. In this study, we isolated a novel PAE-degrading bacteria, Sinomonas sp. S2, from a contaminated area of a metal(loid) smelter in Guangxi Zhuang Autonomous Region, China. Strain S2 is capable of degrading short-chain PAEs, including dimethyl phthalate (DMP), diethyl phthalate (DEP), di-iso-butyl phthalate (DIBP) and di-n-butyl phthalate (DBP). Sinomonas sp. S2 can completely degrade DBP at concentrations of 400 mg·L-1 within 24 h. The degradation kinetics of PAEs followed the modified Gompertz model. Strain S2 demonstrated good environmental adaptability thriving at pH ranging from 5 to 9 and temperatures between 20 and 40 °C, indicated by its growth on DBP. The optimal pH and temperature for degradation were found to be 7 and 40 °C, respectively. Additionally, several metabolites of DBP were identified, including phthalic acid (PA), butyl acetate, ethyl propionate and methyl 2-methylbutyrate. The reconstructed degradation pathway of DBP may involve protocatechuic acid, β-carboxy-cis, cis-mucronate and γ-carboxy muconolactone, ultimately leading to the tricarboxylic acid (TCA). In a bioaugmentation experiment involving soil artificially contaminated with DBP, strain S2 could promote the degradation of DBP in soil. The results indicate that strain S2 had high degradation capacity and environmental tolerance, which had the potential to be applied in the bioremediation of DBP-contaminated environments.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"67"},"PeriodicalIF":3.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metal pollution assessment and bioremediation potential of multidrug-resistant Proteus mirabilis isolated from Buckingham canal, Chennai. 金奈白金汉运河多药耐药变形杆菌重金属污染评价及生物修复潜力

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-18 DOI: 10.1007/s10532-025-10163-z

S Sugitha, G Abirami

{"title":"Heavy metal pollution assessment and bioremediation potential of multidrug-resistant Proteus mirabilis isolated from Buckingham canal, Chennai.","authors":"S Sugitha, G Abirami","doi":"10.1007/s10532-025-10163-z","DOIUrl":"https://doi.org/10.1007/s10532-025-10163-z","url":null,"abstract":"The present study investigates the physicochemical, heavy metal, and microbiological characteristics of water and sediment samples from the Buckingham Canal, Chennai, to assess environmental pollution and explore the bioremediation potential of native bacterial isolates. The water and sediment samples revealed the concentration of heavy metals in the sequence Zn > Mn > Pb > Cu > Cr and Zn > Mn > Cu > Cr > Pb. Among 25 isolates, BCSS04 showed exceptional resistance, tolerating up to 2100 ppm (Pb), 1900 ppm (Zn, Mn, and Cr), and 1300 ppm (Cu), identified as Proteus mirabilis through 16S rRNA sequencing (GenBank accession: PP980976.1). Molecular analysis confirmed the presence of the pbrA gene, while antibiotic susceptibility profiling revealed multidrug resistance, suggesting potential co-selection of metal and antibiotic resistance traits. Growth profiling under metal-induced stress revealed the highest bacterial growth under Mn (0.654 to 0.996) and Pb (0.623 to 0.984). Uptake studies confirmed efficient biosorption capabilities, with peak Pb and Zn uptake reaching 4.23 and 4.21 mg/g, respectively, at 100 ppm. Bioaccumulation assays supported these findings, with maximum accumulation rates for Zn (69.67%) and Pb (67.11%) at 100 ppm, gradually decreasing with increasing concentrations due to saturation or stress effects. SEM and FTIR analyses demonstrated structural and biochemical changes in Proteus mirabilis under metal stress. Molecular docking further revealed strong interactions between heavy metals against Metallothionein SmtA exhibited the strongest interaction with Zn (binding energy: -9.8 kcal/mol), involving eight active residues (TYR A:18, GLY A:53, ASP A:73, ASP A:50, GLU A:55, ARG A:26, HIS A:119, GLY A:52). The integrated physiological, biochemical, and molecular insights affirm the potential of Proteus mirabilis as a promising candidate for bioremediation of heavy metal-contaminated environments.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"63"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing SDS as a source of sulfur: a bioremediation strategy of Fischerella sp. lmga1. 利用SDS作为硫源：Fischerella sp. lmga1的生物修复策略

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-17 DOI: 10.1007/s10532-025-10158-w

Ankit Srivastava, Anirbana Parida, Samujjal Bhattacharjee, Neha Gupta, Satya Shila Singh, Arun Kumar Mishra

{"title":"Harnessing SDS as a source of sulfur: a bioremediation strategy of Fischerella sp. lmga1.","authors":"Ankit Srivastava, Anirbana Parida, Samujjal Bhattacharjee, Neha Gupta, Satya Shila Singh, Arun Kumar Mishra","doi":"10.1007/s10532-025-10158-w","DOIUrl":"https://doi.org/10.1007/s10532-025-10158-w","url":null,"abstract":"Sodium dodecyl sulfate (SDS), a widely used anionic surfactant, is a pervasive aquatic pollutant with documented ecotoxicity and persistence in the environment. In this study, we investigated metabolic response of the filamentous, heterocytous cyanobacterium Fischerella sp. lmga1 under sulfur starvation, focusing on its capacity to degrade SDS and utilize it as an alternative sulfur source. Sulfur-deprived cultures supplemented with 150 µM SDS initially exhibited chlorosis and physiological stress, but showed significant recovery by 14 days, including increased growth and better photosynthetic performance. A significant rise in intracellular sulfur content was observed, suggesting active sulfur acquisition. Expression analysis revealed strong induction of genes involved in sulfur uptake and assimilation (e.g., cysT, cysW, sbp, sat), alongside an ~ 880-fold upregulation of sdsA1 on day 10, encoding an SDS hydrolase. Correlation analyses showed that increased sdsA1 expression coincided with improvements in viability and sulfur status. This underscored a coordinated mechanism of SDS degradation and concomitant sulfur assimilation in Fischerella, indicating towards a novel adaptive strategy. Thus, this study establishes Fischerella as a promising candidate for bioremediation of sulfonated pollutants in aquatic systems and expands the knowledge of metabolic plasticity of cyanobacteria.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"61"},"PeriodicalIF":3.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of methotrexate by Pleurotus ostreatus pellets: effects on morphology and biotransformation insights. 平菇微丸对甲氨蝶呤的生物修复：对形态学和生物转化的影响。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-17 DOI: 10.1007/s10532-025-10161-1

Thabata Montserrat Hernández-Cruz, Karina García-Gutiérrez, Adriana Jazmín Legorreta-Castañeda, Guadalupe Guerra-Sánchez, Dario Rafael Olicón-Hernández

{"title":"Bioremediation of methotrexate by Pleurotus ostreatus pellets: effects on morphology and biotransformation insights.","authors":"Thabata Montserrat Hernández-Cruz, Karina García-Gutiérrez, Adriana Jazmín Legorreta-Castañeda, Guadalupe Guerra-Sánchez, Dario Rafael Olicón-Hernández","doi":"10.1007/s10532-025-10161-1","DOIUrl":"https://doi.org/10.1007/s10532-025-10161-1","url":null,"abstract":"Methotrexate (MTX), a widely used antineoplastic drug, poses significant environmental risks due to its persistence and toxicity. This study evaluated the potential of Pleurotus ostreatus fungal pellets for MTX degradation and investigated the underlying removal mechanisms and morphological effects. Fungal pellets were produced under optimized conditions and exposed to MTX in minimal media. MTX removal kinetics were assessed using HPLC, and associated enzymatic activity was quantified. Pleurotus ostreatus achieved over 90% MTX removal within five days. The removal process was primarily enzymatic, as demonstrated by the ineffectiveness of heat-inactivated biomass and the lack of impact from cytochrome P450 inhibition. Elevated laccase activity coincided with MTX degradation, suggesting its key role in the biotransformation pathway. Mass spectrometry revealed 16 MTX metabolites, including 7-hydroxymethotrexate and 2-oxoglutaric acid, indicating progressive oxidation, ring cleavage, hydroxylation, and eventual integration into primary metabolism. Scanning electron microscopy showed significant structural degradation of fungal pellets during the process, highlighting stress-induced morphological changes. The findings establish the feasibility of employing P. ostreatus pellets as an environmentally compatible biotechnological tool for the degradation of cytotoxic compounds like MTX. To our knowledge, this is the first report of MTX biotransformation by fungal pellets of P. ostreatus, and it contributes valuable insights into fungal-based bioremediation strategies for pharmaceutical pollutants.","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 4","pages":"62"},"PeriodicalIF":3.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of As, Cr, Hg, and Mn by Pseudomonadota: chances and risks. 假单胞菌对砷、铬、汞和锰的生物转化：机会和风险。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-15 DOI: 10.1007/s10532-025-10157-x

Aleksandra Naziębło, Jakub Dobrzyński

引用次数: 0

Correction: Physiological and transcriptomic response of enriched anammox culture upon elevated hydrazine exposure. 更正：高浓度厌氧氨氧化培养在高联氨暴露下的生理和转录组反应。

IF 3.1 4区生物学

Biodegradation Pub Date : 2025-07-08 DOI: 10.1007/s10532-025-10151-3

Tugba Sari, Kozet Yapsakli, Deniz Akgul, Bulent Mertoglu

引用次数: 0