Biodegradation最新文献

Effect of urea concentration on the combined pollution of Cd and Ni in microbiologically induced calcite precipitation (MICP) treatment. 微生物诱导方解石沉淀（MICP）处理中尿素浓度对Cd和Ni复合污染的影响

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-22 DOI: 10.1007/s10532-025-10204-7

Dejun Yang, Shengnan Bei, Yong Yang

{"title":"Effect of urea concentration on the combined pollution of Cd and Ni in microbiologically induced calcite precipitation (MICP) treatment.","authors":"Dejun Yang, Shengnan Bei, Yong Yang","doi":"10.1007/s10532-025-10204-7","DOIUrl":"https://doi.org/10.1007/s10532-025-10204-7","url":null,"abstract":"<p><p>Soil heavy metal contamination is one of the most severe global environmental challenges today. Microbiologically induced calcite precipitation (MICP), as an environmentally friendly bioremediation technique, demonstrates significant potential in addressing such pollution. To optimize the MICP process, the research systematically investigated the influence of urea concentration on the remediation of soils co-contaminated with cadmium (Cd) and nickel (Ni). A highly efficient urease-producing strain, SX4 (Enterobacter sp.), was isolated from mining areas, showing the highest urease activity (conductivity change: 22.14 mS·cm⁻<sup>1</sup>) among all isolates. Under optimal growth conditions (pH = 7, urea concentration 20 g·L⁻<sup>1</sup>, OD<sub>600</sub> = 1.76), the remediation cycles for Cd- and Ni-contaminated soils were 120 h and 132 h, respectively. Evaluation of different urea concentrations (0, 10, 20, 40 g·L⁻<sup>1</sup>) confirmed that the 20 g·L⁻<sup>1</sup> group was the most effective. It achieved effective removal rates of 45.71% and 43.34% for Cd and Ni, respectively, in single-pollutant contamination, and 32.44% for Cd and 38.75% for Ni, in co-polluted conditions. The findings elucidate the pivotal role of urea concentration in the MICP remediation process, providing crucial scientific evidence for optimizing the practical engineering parameters of this technology.</p>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 6","pages":"107"},"PeriodicalIF":3.2,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorisation of whey by mixotrophic and heterotrophic cultivation of microalgae for biomass and lipid production 混合营养和异养培养微藻用于生物质和脂质生产的乳清价值

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-16 DOI: 10.1007/s10532-025-10203-8

Oto Jekabs Apse, Kristaps Neiberts, Santa Sukaruka, Tomass Tumpelis, Pavels Semjonovs

{"title":"Valorisation of whey by mixotrophic and heterotrophic cultivation of microalgae for biomass and lipid production","authors":"Oto Jekabs Apse, Kristaps Neiberts, Santa Sukaruka, Tomass Tumpelis, Pavels Semjonovs","doi":"10.1007/s10532-025-10203-8","DOIUrl":"10.1007/s10532-025-10203-8","url":null,"abstract":"<div><p>The dairy industry generates large volumes of whey, a nutrient rich by-product that is a threat to the environment because of a high lactose concentration. In this research, a potential of four microalgae strains – <i>Tetradesmus obliquus</i> MSCL 1710, <i>Graesiella emersonii</i> MSCL 1711, <i>Chlorella vulgaris</i> CCAP 211/111 and <i>Scenedesmus quadricauda</i> CCAP 276/16 was investigated to evaluate their growth under heterotrophic and mixotrophic conditions of lactose and its monosaccharides. This work specifically compares sweet whey (SW) and acid whey (AW) as a promising substrates for microalgal growth and lipid synthesis. Peculiar focus was given to local isolates—<i>G. emersonii</i> MSCL 1711 and <i>T. obliquus</i> MSCL 1710 cultivated in sweet (SW) and acidic whey (AW) at different concentrations and temperatures (15 °C and 25 °C). Mixotrophic cultivation in SW enhanced biomass productivity, with <i>G. emersonii</i> MSCL 1711 achieving 0.30 g/L/d and <i>T. obliquus</i> MSCL 1710 0.29 g/L/d at 25 °C. The highest lipid accumulation was observed in SW for <i>G. emersonii</i> MSCL 1711 at 15 °C, representing a 158.31% increase compared with the autotrophic control (mg/g dry weight). Additionally, β – galactosidase activity correlated with lactose assimilation in the medium for both cultures, suggesting that it is suitable for cultivation on whey substrates. The results confirm the ability of local microalgal strains to grow on SW, and on AW, demonstrating their potential for dairy by-product bioconversion into microalgal biomass for broad applications.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145296611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity of Phanerochaete chrysosporium during degradation of diesel fuel-contaminated soil under solid-state fermentation: CO2 production and ligninolytic enzymes 固态发酵下黄孢平革菌降解柴油污染土壤的活性：CO2产和木质素分解酶

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-15 DOI: 10.1007/s10532-025-10202-9

Atefeh Etemadi-Khah, Ahmad Ali Pourbabaee

{"title":"Activity of Phanerochaete chrysosporium during degradation of diesel fuel-contaminated soil under solid-state fermentation: CO2 production and ligninolytic enzymes","authors":"Atefeh Etemadi-Khah, Ahmad Ali Pourbabaee","doi":"10.1007/s10532-025-10202-9","DOIUrl":"10.1007/s10532-025-10202-9","url":null,"abstract":"<div><p>Diesel fuel is a complex petroleum compound that is considered an important and serious risk for organisms and their environment. There are different methods for soil cleaning from this compound, of which bioremediation is one of the best. This study was conducted to investigate the efficiency of <i>Phanerochaete chrysosporium</i> fungus in bioremediation of diesel fuel contaminated soil under oxygen-deficient conditions. In this study, soil samples free of petroleum compounds were manually contaminated with 3000 mg/kg of diesel fuel and incubated for 60 days at 30°C in a dark chamber. The amounts of CO<sub>2</sub> production, microbial growth, manganese peroxidase, and lignin peroxidase enzyme activities were measured every 10 days. The results indicated that the amount of CO<sub>2</sub> production in both pure and mixed cultures increased significantly from the beginning of the experiment to the 40th day, which was 22.96 and 25.53 mg/g/w, respectively. Manganese peroxidase and lignin peroxidase enzymes also first reached their maximum values on the 40th day, which were 225 U/L and 31.5 U/L, respectively, and then decreased. The average percentage of TPH degradation on different days showed that the biological decontamination rate of diesel fuel in pure and mixed culture was 79.62 and 83.17%, respectively, within 60 days. By comparing the biodegradation rate with other data, we concluded that <i>P. chrysosporium</i> can degrade diesel fuel under fermentation conditions and use its compounds to provide energy and carbon.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145296790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial phytoremediation of contaminated soils irrigated with industrial effluents: a soil health perspective 用工业废水灌溉污染土壤的微生物植物修复：土壤健康观点。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-11 DOI: 10.1007/s10532-025-10201-w

Amina Rasheed, Naila Khan, Nabila Shehzadi, Sajjad Hyder, Zarrin Fatima Rizvi, Amjad Shahzad Gondal, Noreen Khalid, Pamela Helué Morales-Sandoval, Sergio de los Santos-Villalobos, Lala Gurbanova, Rashid Iqbal, Mehdi Rahimi

{"title":"Microbial phytoremediation of contaminated soils irrigated with industrial effluents: a soil health perspective","authors":"Amina Rasheed, Naila Khan, Nabila Shehzadi, Sajjad Hyder, Zarrin Fatima Rizvi, Amjad Shahzad Gondal, Noreen Khalid, Pamela Helué Morales-Sandoval, Sergio de los Santos-Villalobos, Lala Gurbanova, Rashid Iqbal, Mehdi Rahimi","doi":"10.1007/s10532-025-10201-w","DOIUrl":"10.1007/s10532-025-10201-w","url":null,"abstract":"<div><p>Industrialization, urbanization, and poor farming practices have led to major problems regarding potentially toxic elements (PTEs). PTEs in industrial effluents adversely affect water quality, soil, plants, and aquatic life, and ultimately cause severe health problems in humans. Several strategies have been utilized to overcome this serious environmental issue. The conventional methods most commonly used for this purpose are expensive and not environmentally friendly. Phytoremediation is a very cost-effective and eco-friendly strategy where researchers are focusing their efforts nowadays. This technique utilizes plants to remove PTEs from the soil. The efficacy of phytoremediation is enhanced by the microorganisms in the rhizosphere, where microbes utilize root exudates as their energy source, which in turn remove or solubilize PTEs from the soil. Microbes have adopted several mechanisms that directly and/or indirectly assist plants in resisting PTE stress. These mechanisms include biosorption, bioaccumulation, efflux systems, enzymatic detoxification, siderophore production, biosurfactants, extracellular sequestration, intracellular sequestration, ACC-deaminase, IAA production, and phytohormone production. Plant–microbe interaction is one of the most successful approaches that not only aids in remediating PTEs from the soil but also assists plant development. The efficiency of microbial activity could be enhanced by inserting PTE resistance genes so that genetically engineered microbes (GEMs) work more efficiently to remove PTEs from soil or water. The current review addresses the deleterious effects of PTEs on living organisms and discusses possible cost-effective and eco-friendly microbial-assisted phytoremediation strategies to remove PTEs from soil contaminated with industrial effluents.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel association by Rhodospirillum rubrum and Rhodobacter capsulatus for bioremediation of heavy metals from aqueous solutions 红红螺旋菌与荚膜红杆菌的新型联合生物修复水溶液中重金属

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-10 DOI: 10.1007/s10532-025-10199-1

Daniela Aparecida Santos, Gabriela Aparecida Santos, Miriam Maria de Resende, Larissa Nayhara Soares Santana Falleiros, Fabiana Regina Xavier Batista

{"title":"A novel association by Rhodospirillum rubrum and Rhodobacter capsulatus for bioremediation of heavy metals from aqueous solutions","authors":"Daniela Aparecida Santos, Gabriela Aparecida Santos, Miriam Maria de Resende, Larissa Nayhara Soares Santana Falleiros, Fabiana Regina Xavier Batista","doi":"10.1007/s10532-025-10199-1","DOIUrl":"10.1007/s10532-025-10199-1","url":null,"abstract":"<div><p>Bioremediation is a promising method for removing heavy metals in contaminated effluents. Using several microorganisms, the process can provide efficient treatment, resulting in reduced waste generation, all while promoting sustainability. The current work evaluated the potential of the novel assembly by <i>Rhodospirillum rubrum</i> and <i>Rhodobacter capsulatus</i> to remove hexavalent chromium, total chromium, cadmium, and lead. In addition, photosynthetic pigment (bacteriochlorophyll-<i>a</i> and carotenoids) production and biomass increment were verified. A composite central design (CCD) was proposed to obtain models describing the behavior of the initial concentration of chromium, cadmium, and lead (independent variables). In the experiments described at the central point by the CCD, the co-culture (<i>R. capsulatus: R. rubrum</i>) was inoculated in 500 mL Erlenmeyer flasks containing an effluent consisting of RCV medium with heavy metals (20 mg/L Cr<sup>6+</sup>, 10 mg/L Cd<sup>2+</sup> and 10 mg/L Pb<sup>2+</sup>). With a light intensity of 5760 lx and a biological cycle of 216 h, the maximum removals were 83% for total chromium, 30% for cadmium, and 80% for lead. Under these conditions, the biomass increased by 68% compared to the initial value (1.0 g/L), even in a highly toxic effluent.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filamentous fungal-mediated melanin nanoparticles for heavy metal detoxification via bioadsorption: a sustainable approach 丝状真菌介导的黑色素纳米颗粒通过生物吸附对重金属解毒：一种可持续的方法。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-07 DOI: 10.1007/s10532-025-10198-2

Michael Helan Soundra Rani, Sivakumar Sujith

{"title":"Filamentous fungal-mediated melanin nanoparticles for heavy metal detoxification via bioadsorption: a sustainable approach","authors":"Michael Helan Soundra Rani, Sivakumar Sujith","doi":"10.1007/s10532-025-10198-2","DOIUrl":"10.1007/s10532-025-10198-2","url":null,"abstract":"<div><p>The eradication of heavy metal contamination has emerged as a paramount objective in preserving and conserving global water resources. The present study highlights the potential of halophilic fungal melanin derived from <i>Curvularia lunata</i> as an eco-friendly, cost-effective, highly stable, and efficient biosorbent for removing toxic heavy metals. UV and FTIR spectroscopy characterization confirmed the presence of functional groups typical of eumelanin. Particle size analysis revealed a notable reduction in size from unmodified melanin (54.22–87.94 nm) to melanin nanoparticles (MNPs) (22.74–26.41 nm), indicating improved surface area for adsorption. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) data further validated the superior adsorption capabilities of MNPs compared to unmodified melanin. Specifically, the MNPs exhibited a 100% removal efficiency of over 18 metals out of 24 at a concentration of 0.15 mg/L and at pH 7, surpassing the performance of native melanin. X-ray photoelectron spectroscopy (XPS) was applied to specify the elemental composition of the solid surfaces and the chemical forms of adsorbed metals. Ultrasound-assisted extraction (UAE) significantly enhances adsorption efficacy by facilitating better dispersion and generating a higher surface area, thereby increasing the Number of active binding sites available on MNPs for heavy metal chelation. This mycoremediation-based approach presents a scalable and industrially adaptable solution for water detoxification, offering an advantageous alternative to conventional high-performance membrane technologies with minimal process modifications.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved hydrolyzed polyacrylamide biodegradation and gel breaking performance of composite bacteria 提高了复合菌对水解聚丙烯酰胺的生物降解和破胶性能。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-05 DOI: 10.1007/s10532-025-10196-4

Jiqian Wang, Xuanyu Zhang, Han Han, Yuqin Jia, Hai Zhang, Yanhui Jia, Hui Li

{"title":"Improved hydrolyzed polyacrylamide biodegradation and gel breaking performance of composite bacteria","authors":"Jiqian Wang, Xuanyu Zhang, Han Han, Yuqin Jia, Hai Zhang, Yanhui Jia, Hui Li","doi":"10.1007/s10532-025-10196-4","DOIUrl":"10.1007/s10532-025-10196-4","url":null,"abstract":"<div><p>Polymer flooding technology enhances crude oil recovery but generates a large amount of wastewater containing hydrolyzed polyacrylamide (HPAM) and HPAM residue in oil reservoirs, which induce serious environmental problems. Effective degradation of HPAM is highly required in oilfields, especially biodegradation technologies. Ten strains of HPAM degrading bacteria have been screened and identified from oilfield wastewater. An optimal HPAM biodegradation system of composite bacteria has been established based on two strains <i>Agrobacterium pusense</i> NMYGYA2 and <i>Stutzerimonas balearica</i> SCE1. The HPAM biodegradation performance of the composite bacteria has been improved through cultivation condition optimization. The highest HPAM removal rate of 81.2% could be achieved at the optimized condition with the addition of 800 mg·L<sup>−1</sup> urea, 500 mg·L<sup>−1</sup> glucose and 50 mg·L<sup>−1</sup> CaCl<sub>2</sub>. The gel permeation chromatography results showed that the HPAM molecular weight decreased from 3.7 × 10<sup>6</sup> Da to 1.9 × 10<sup>5</sup> Da after the composite bacterial degradation. Fourier transform infrared spectroscopy analysis revealed the hydrolysis of NH<sub>2</sub> group and the cleavage of C–C bond. Furthermore, the composite bacteria exhibited the ability to break down HPAM gels via biodegradation at temperatures up to 55 °C, indicating that they can be used to treat the blocking in reservoirs with a temperature < 55 °C and ground facilities.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial degradation of diesel fuel as a potential environmental bioremediation 微生物降解柴油作为一种潜在的环境生物修复技术。

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-04 DOI: 10.1007/s10532-025-10194-6

Letícia Beatriz Ueda Melo, Bruna Bacaro Borrego, Louise Hase Gracioso, Elen Aquino Perpetuo

{"title":"Microbial degradation of diesel fuel as a potential environmental bioremediation","authors":"Letícia Beatriz Ueda Melo, Bruna Bacaro Borrego, Louise Hase Gracioso, Elen Aquino Perpetuo","doi":"10.1007/s10532-025-10194-6","DOIUrl":"10.1007/s10532-025-10194-6","url":null,"abstract":"<div><p>Diesel oil is a fossil fuel widely utilized globally and a significant source of environmental contamination. Its presence poses substantial ecological challenges. Bioremediation emerges as a viable solution for restoring diesel-contaminated environments, contingent upon comprehending the local microbiota. This study employed a metabarcoding technique with a culture-dependent approach to assess the impact of hydrocarbon contamination on soil microbiota. Soil samples were collected from contaminated areas, and microbial diversity was assessed through relative abundance, alpha, and beta diversity analyses. Additionally, bacterial strains isolated from the same area were screened for their ability to degrade hydrocarbons. Four strains, <i>Pseudomonas nitroreducens</i> B32, <i>Pseudomonas koreensis</i> B11, <i>Ralstonia</i> sp. BC2, and <i>Acinetobacter</i> sp. BC3 could degrade up to 38% of the diesel, using it as the sole carbon source. These strains effectively degraded n-alkanes and cyclic alkanes with short and medium chains (C7 to C18). This research enhances the understanding of hydrocarbons’ impacts on soil microbiota and underscores the potential application of microorganisms in bioremediation efforts.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation potential assessment of oil-degrading bacterial consortium prepared from local bacterial strains and its survival using different porous carriers 本地菌株制备的石油降解菌群的生物修复潜力评价及其在不同多孔载体下的存活率

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-03 DOI: 10.1007/s10532-025-10188-4

Sareh Sadeghianfard Boroojeni, Hossein Motamedi

{"title":"Bioremediation potential assessment of oil-degrading bacterial consortium prepared from local bacterial strains and its survival using different porous carriers","authors":"Sareh Sadeghianfard Boroojeni, Hossein Motamedi","doi":"10.1007/s10532-025-10188-4","DOIUrl":"10.1007/s10532-025-10188-4","url":null,"abstract":"<div><p>Oil sludge contains high levels of heavy chain petroleum hydrocarbons and heavy metals that hinder its biodegradation. Hence for successful remediation, selecting potent isolates and construction of efficient bacterial consortium is inevitable. The aim was to achieve bacterial consortium with the ability to tolerate harsh environment of oil sludge and degrade different hydrocarbon fractions of it. For this purpose, native oil-degrading and biosurfactant-producing bacteria were screened from oil tanks bottom sludge and were evaluated for their salt and heavy metal tolerance. Also, oil-degrading potentials of the isolates as well as their consortium were assessed through GC-FID analysis under both static and shaking conditions. The potential of sugarcane vinasse as a low-cost culture medium for large scale culture of the isolates as well as their immobilization and long-term viability on porous carriers including diatomaceous earth, sugarcane bagasse, and biochar were also investigated. The results showed that <i>A. lactucae</i> strain Ib-30 had the highest hydrocarbon degradation (~ 77%) and high level metal resistance. The oil-degrading efficiency of bacterial consortium was lower than that of individual isolates. <i>S. warneri</i> strain Ae1-30 was identified as the most halotolerant and metal-resistant isolate. Vinasse supported the growth of all strains, with <i>C. hisashii</i> strain T1-50 showing the highest proliferation rate. Sugarcane bagasse outperformed other carriers in maintaining bacterial viability over 14 months. Overall, these findings demonstrate the feasibility of scalable, sustainable bioremediation of oil sludge using potent indigenous bacterial resources and effective bio-carriers, offering a promising solution for industrial waste management.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145210427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism underlying lignite biodegradation by Cupriavidus sp isolated from sludge 污泥中铜毒杆菌降解褐煤的机制

IF 3.2 4区生物学

Biodegradation Pub Date : 2025-10-03 DOI: 10.1007/s10532-025-10191-9

Yaya Wang, Weilong Cao, Tianyu Zhu, Jiaxuan Li, Damir Nussipov, Kuanysh Tastambek, Xiangrong Liu

{"title":"The mechanism underlying lignite biodegradation by Cupriavidus sp isolated from sludge","authors":"Yaya Wang, Weilong Cao, Tianyu Zhu, Jiaxuan Li, Damir Nussipov, Kuanysh Tastambek, Xiangrong Liu","doi":"10.1007/s10532-025-10191-9","DOIUrl":"10.1007/s10532-025-10191-9","url":null,"abstract":"<div><p>Lignite, a low-rank coal, is commonly utilized as a fuel source. However, its high sulfur and ash content can result in the release of harmful substances during combustion. Microbial coal degradation offers a more environmentally friendly alternative to traditional chemical and physical methods of coal treatment. In this study, we obtained a bacterium, named as <i>Cupriavidus sp</i> isolated from activated sludge that exhibits potential for lignite degradation. After identification via 16S rDNA sequencing, the degradation characteristics and mechanisms of strain S4 on lignite from Shanxi, were systematically evaluated. Extracellular enzyme activities of strain S4 were measured, revealing the secretion of lignin peroxidase, manganese peroxidase, laccase, alkaline protease, and amylase, indicating its capacity for multi-enzyme synergistic degradation. Scanning electron microscopy (SEM) observations confirmed that the bacterium could adsorb onto the coal surface. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated a significant increase in free hydroxyl groups on the coal, which facilitates degradation. Gas chromatography-mass spectrometry (GC–MS) and three-dimensional fluorescence spectroscopy analyses of the liquid-phase products showed a notable increase in long-chain alkanes and phenolic compounds in the degradation liquid, along with the detection of humic substances. Further studies indicated that strain S4 mediates initial adsorption through the secretion of extracellular polymers (EPS) rich in proteins and polysaccharides, highlighting the key mechanism of microbial-coal interface interaction. This study provides a theoretical foundation for the development of lignite bioremediation technologies and the resource-based application of functional bacterial strains.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145210613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0