Biodegradation最新文献

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A comparative study using response surface methodology and artificial neural network for modeling the bio-reduction of hexavalent chromium (Cr⁶⁺) by immobilized cells of Paenibacillus taichungensis strain MAHA in an alginate-gellan gum matrix
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-04-01 DOI: 10.1007/s10532-025-10124-6
Maha Obaid Al-Osaimi, Mohd Izuan Effendi Halmi, Siti Salwa Abd Gani, Khairil Mahmud, Mohd Yunus Abd Shukor
{"title":"A comparative study using response surface methodology and artificial neural network for modeling the bio-reduction of hexavalent chromium (Cr⁶⁺) by immobilized cells of Paenibacillus taichungensis strain MAHA in an alginate-gellan gum matrix","authors":"Maha Obaid Al-Osaimi,&nbsp;Mohd Izuan Effendi Halmi,&nbsp;Siti Salwa Abd Gani,&nbsp;Khairil Mahmud,&nbsp;Mohd Yunus Abd Shukor","doi":"10.1007/s10532-025-10124-6","DOIUrl":"10.1007/s10532-025-10124-6","url":null,"abstract":"<div><p>Chromium (Cr⁶⁺) waste poses a hazard as it leads to imbalanced ecosystems and severe health issues. Although, it is widely associated with many industries. Chromium (Cr⁶⁺) reduction by the immobilized cells of <i>Paenibacillus taitungensis</i> strain MAHA-MIE was optimized using response surface methodology (RSM) and artificial neural networks (ANN). The RSM-Box-Behnken Design (BBD) was selected to investigate the effects of chromium (Cr⁶⁺) concentration, alginate concentration, gellan gum concentration, bead size, and the number of beads on chromium (Cr⁶⁺) reduction rate. Experimental data from the BBD was used to train a feed-forward, multilayer artificial neural network (ANN). Results show that the ANN model outperformed the response surface methodology (RSM) based on actual and predicted data, with lower errors and a higher R<sup>2</sup> value. The ANN model predicted the optimum points as follows: 155 ppm chromium (Cr⁶⁺), 0.32% alginate, 0.65% gellan gum, 0.5 cm beads, and 27 beads. The validation confirmed a high agreement of chromium (Cr⁶⁺) reduction rate between the validation value (99.00%) and the predicted value (99.99%), with the lowest deviation at 0.1%. Modeling abilities were compared using statistical criteria, including Root Mean Square Error (RMSE), Standard Error of Prediction (SEP), Relative Percent Deviation (RPD), and regression coefficients (R<sup>2</sup>). The ANN analysis showed the high predictive performance, with high R<sup>2</sup> (0.9911), low SEP (0.45%), RPD (1.88), and RMSE (1.37%). The results of this study approved that alginate-gellan gum immobilized cells of <i>Paenibacillus taitungensis</i> strain MAHA-MIE could be effectively used for the handling of chromium (Cr⁶⁺).</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comprehensive review on microbial production and significant applications of multifunctional biomolecules: biosurfactants
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-30 DOI: 10.1007/s10532-025-10121-9
Nisha Sharma, Yogesh K. Ahlawat, Arti Jamwal Sharma, Navneeti Chamoli, Monika Thakur, Anupriya Sharma, Sajid Mehmood, Anurag Malik, Meraj Ahmed, Himani Punia, Sumati Choubey
{"title":"A comprehensive review on microbial production and significant applications of multifunctional biomolecules: biosurfactants","authors":"Nisha Sharma,&nbsp;Yogesh K. Ahlawat,&nbsp;Arti Jamwal Sharma,&nbsp;Navneeti Chamoli,&nbsp;Monika Thakur,&nbsp;Anupriya Sharma,&nbsp;Sajid Mehmood,&nbsp;Anurag Malik,&nbsp;Meraj Ahmed,&nbsp;Himani Punia,&nbsp;Sumati Choubey","doi":"10.1007/s10532-025-10121-9","DOIUrl":"10.1007/s10532-025-10121-9","url":null,"abstract":"<div><p>Microorganisms are very well known potential sources of many novel metabolites and biosurfactants (green molecules). Biosurfactants are biobased molecules which are synthesized by bacteria, yeasts, fungi and actinomycetes. These biomolecules have emerged as multifunctional biomolecules of the 21st century due to their remarkable functional properties like low toxicity, enhanced effectiveness, selectivity, stability, high biodegradability and eco-friendly nature. These characteristics enable them to remain high effective under extreme conditions and play a significant role in environmental protection. Biosurfactants play a pivotal role in bioremediation technologies, offering an environmentally sustainable alternative for cleaning up contaminants. Their unique ability to reduce interfacial tension in liquids enables them to perform crucial functions such as biodegradation, emulsification, foam formation, surface activity, washing performance and detergent formulation. These versatile properties make biosurfactants invaluable across various industries, including environmental remediation, pharmaceuticals, agriculture and cosmetics. This review discusses the microbial production, characterization, industrial applications and ecological significance of biosurfactants. By highlighting their impact in the bioremediation of contaminants, this article underscores the potential of biosurfactants in advancing green technologies and addressing global environmental challenges.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Challenges of exopolysaccharides production from polystyrene degradation by bacterium CHB 1.5 strain
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-30 DOI: 10.1007/s10532-025-10122-8
Saijai Wattanasen, Pajongsuk Sutarut, Areeya Taengnoi, Salwa Torpee
{"title":"Challenges of exopolysaccharides production from polystyrene degradation by bacterium CHB 1.5 strain","authors":"Saijai Wattanasen,&nbsp;Pajongsuk Sutarut,&nbsp;Areeya Taengnoi,&nbsp;Salwa Torpee","doi":"10.1007/s10532-025-10122-8","DOIUrl":"10.1007/s10532-025-10122-8","url":null,"abstract":"<div><p>Polystyrene (PS), a substance that constitutes a significant portion of plastic waste, has resulted in environmental pollution and adverse health effects. Biodegradation and chemical transformation of PS are limited. However, biodegradation is one alternative way to reduce plastic pollution. This research aims to select plastic-degrading bacteria and produce exopolysaccharides (EPS) from plastic waste. Among the marine plastic waste at Chala tat Beach (Songkhla, Thailand), 35 rod-shaped and Gram-positive bacteria were found. The selected strains that exhibited the highest optical density (OD) at 600 nm were CHB1.5, CHD2.2, and CHC3.2. The efficiency of EPS production was tested and showed that CHB 1.5 could produce the maximum amount of EPS (13.47 ± 0.10 g/L) with a significant difference. After four weeks of plastic breakdown, CHB 1.5 had the highest total count (4.03 ± 0.02 Log CFU/mL), followed by CHD2.2 and CHC3.2 (3.99 ± 0.12 and 3.96 ± 0.02 Log CFU/mL, respectively). CHB 1.5 was also examined to use PS foam as a carbon source in modified Mineral Salt Medium for EPS production, with an EPS yield of 1.36 ± 0.08 g/L in week 4. The presence of amides I, polysaccharides, benzene rings, and hydroxyl groups (O–H) was detected by Fourier transform infrared spectroscopy. The Scanning Electron Microscope images confirmed the adherence of the CHB1.5 strain and EPS formation on the plastic sheet. In conclusion, the strain CHB1.5 showed promising potential for degrading PS plastic and producing EPS. Its qualities could be utilized in the future, as well as contribute to the reduction of plastic pollution in the environment in an eco-friendly way.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced bio-reduction of Cr(VI) using Shewanella putrefaciens CN32 mediated by Fe(III) minerals and riboflavin synergistically
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-25 DOI: 10.1007/s10532-025-10120-w
Tianle Zhang, Haibo Li, Yichen Wu, Yajue Yuan, Yu Du
{"title":"Enhanced bio-reduction of Cr(VI) using Shewanella putrefaciens CN32 mediated by Fe(III) minerals and riboflavin synergistically","authors":"Tianle Zhang,&nbsp;Haibo Li,&nbsp;Yichen Wu,&nbsp;Yajue Yuan,&nbsp;Yu Du","doi":"10.1007/s10532-025-10120-w","DOIUrl":"10.1007/s10532-025-10120-w","url":null,"abstract":"<div><p>Iron minerals and the coupling of electron shuttle media can effectively overcome the problem of the insolubility of iron minerals and the higher cross-medium resistance consequently to enhance the bio-reduction rate of Cr(VI) by dissimilatory metal-reducing bacteria (DMRB). This study explored the potential synergistic enhancement of Cr(VI) bio-reduction by <i>Shewanella putrefaciens</i> CN32 in combination with three iron minerals (ferrihydrite, goethite and hematite) and riboflavin (RF). The addition of RF accelerates the transfer of electrons from bacterial cells to Fe minerals, which in turn promotes the production of large amounts of Fe(II). The results indicated that compared to the control group, the Cr(VI) reduction rates in the CN32/RF/hematite, goethite, ferrihydrite systems increased to 93.03%, 91.07%, and 86.83%, hematite was capable of generating 2.24 mM Fe(II) due to its stable structure and efficient synergy with riboflavin. Enhancement factor(EF) was used to quantify the synergistic effect of RF and iron minerals on the bio-reduction of Cr(VI). At all three reaction times, the F<sub>EF</sub> (K<sub>CN32+RF+Fe</sub>/K<sub>CN32</sub>) of three Fe(III) minerals were all greater than 1. XPS analysis revealed that the primary reduction products of Cr(VI) were identified as Cr(CH<sub>3</sub>C(O)CHC(O)CH<sub>3</sub>)<sub>3</sub>, Cr<sub>2</sub>O<sub>3</sub> and Fe(II)-Cr(III) hydroxide, were predominantly deposited on both bacterial and mineral surfaces, thereby influencing their synergistic interactions. This study unveiled the dynamic synergistic mechanism changes of Cr(VI) reduction in different iron minerals environment,which offers new ideas for the remediation of Cr(VI) pollution.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent perspectives on biotechnological production, modulation and applications of glycerophosphoryl diester phosphodiesterases
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-14 DOI: 10.1007/s10532-025-10119-3
Arshia Nazir, Muhammad Sajjad
{"title":"Recent perspectives on biotechnological production, modulation and applications of glycerophosphoryl diester phosphodiesterases","authors":"Arshia Nazir,&nbsp;Muhammad Sajjad","doi":"10.1007/s10532-025-10119-3","DOIUrl":"10.1007/s10532-025-10119-3","url":null,"abstract":"<div><p>Organophosphate (OP) compounds have been extensively employed as pesticides, insecticides and nerve agents. Stockpiles of chemical warfare agents must be destroyed as recommended by Chemical Weapon Convention (CWC). Toxicity of OP compounds to insects and mammals is due to their ability to inhibit the activity of acetylcholinesterase. Accumulation of acetylcholine leads to overstimulation of nerves, leading to convulsion, paralysis or even death. There is a dire need to decontaminate OP contaminated sites by using inexpensive and eco-friendly agents. Recently, OP hydrolyzing enzymes such as glycerophosphoryl diester phosphodiesterases (GDPDs) emerged as appealing agents to clean-up OP contaminated environmental sites. GDPDs are well known for enzymatic generation of glycerol 3-phosphate and corresponding alcoholic moiety from glycerophosphodiesters. Additionally, they are also involved in hydrolysis of OP compounds and degradative products of nerve agents. In the current review, structural and functional characteristics of GDPDs have been elaborated. Production of GDPDs from natural sources is quiet low so the current study aims at recombinant production of GDPDs from various sources. Comparative analysis of biochemical characteristics of various GDPDs indicated that thermostable GDPDs are active over broad temperature and pH range. In addition, thermostable GDPDs are resistant to high concentrations of organic solvents as well as metal ions. In order to enhance their practical utility, different engineering approaches (directed evolution, rational design and site-saturation mutagenesis) as well as immobilization strategies can be utilized to improve catalytic properties of GDPDs. Thus, the current review highlights the utilization of recombinant engineered free or immobilized GDPDs as tools in OP bioremediation.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in actinobacteria-based bioremediation: mechanistic insights, genetic regulation, and emerging technologies
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-14 DOI: 10.1007/s10532-025-10118-4
Naureenbanu Makarani, Radhey Shyam Kaushal
{"title":"Advances in actinobacteria-based bioremediation: mechanistic insights, genetic regulation, and emerging technologies","authors":"Naureenbanu Makarani,&nbsp;Radhey Shyam Kaushal","doi":"10.1007/s10532-025-10118-4","DOIUrl":"10.1007/s10532-025-10118-4","url":null,"abstract":"<div><p>Untreated wastewater from sewage, industries, and agriculture contaminates ecosystems due to rapid population growth and industrialization. It introduces hazardous pollutants, including pesticides, polycyclic aromatic hydrocarbons (PAHs), and heavy metals, which pose serious health risks such as cancer, lung disorders, and kidney damage, threatening both environmental and human well-being. Using microorganisms for bioremediation is thought to be safer and more effective. Compared to other approaches, bioremediation is the most effective way to absorb heavy metals. Due to the high cost and unreliability of traditional remediation techniques, such as chemical and physical treatments, interest in bioremediation as an environmentally benign substitute has grown. Through the use of microorganisms, bioremediation successfully removes heavy metals and breaks down organic contaminants from contaminated circumstances. Actinobacteria are unique among these microbes because of their flexibility in metabolism and capacity to endure severe environments. They create secondary metabolites, such as enzymes, that help break down a variety of pollutants. Actinobacteria also produce siderophores and extracellular polymeric substances (EPS), which aid in trapping organic contaminants and immobilizing heavy metals. This review explores the diverse applications of actinobacteria in bioremediation, with a focus on their mechanisms for breaking down and neutralizing pollutants. We highlighted the advancements in bioremediation strategies, including the use of mixed microbial cultures, biosurfactants, nanoparticles and immobilized cell technologies which enhance the efficiency and sustainability of pollutant removal. The integration of omics technologies such as metagenomics, meta-transcriptomics, and meta-proteomics provides deeper insights into the genetic and metabolic pathways involved in bioremediation, suggesting the way for the development of genetically optimized strains with enhanced degradation capabilities. By leveraging these emerging technologies and microbial strategies, actinobacteria-mediated bioremediation presents a highly promising approach for mitigating environmental pollution. Ongoing research and technological advancements in this field can further enhance the scalability and applicability of bioremediation techniques, offering sustainable solutions for restoring contaminated ecosystems and protecting human health.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochar mediated fixation of nitrogen compounds (ammonia and nitrite) in soil: a review
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-03-06 DOI: 10.1007/s10532-025-10116-6
Arti Chamoli, Santosh Kumar Karn, Moni Kumari, Elayaraja Sivaramasamy
{"title":"Biochar mediated fixation of nitrogen compounds (ammonia and nitrite) in soil: a review","authors":"Arti Chamoli,&nbsp;Santosh Kumar Karn,&nbsp;Moni Kumari,&nbsp;Elayaraja Sivaramasamy","doi":"10.1007/s10532-025-10116-6","DOIUrl":"10.1007/s10532-025-10116-6","url":null,"abstract":"<div><p>Biochar (BC) is a carbon-rich material created from biomass pyrolysis. It is an efficient addition for reducing ammonia inhibition due to its large specific surface area, porosity, conductivity, redox characteristics, and functional groups making it favorable for both soil and water remediation. The efficacy of biochar on the N cycle is associated with biochar properties which are mainly affected by feedstock type and pyrolysis condition. The addition of BC to soil affects nitrogen adsorption pathways. Other advantages include improved soil fertility, nutrient immobilization, and slow-release carbon storage. Biochar adsorption of ammonia reduces ammonia (NH<sub>3</sub>) and nitrate (NO<sub>3</sub>) losses during composting after manure applications and provides a method for creating slow-release fertilizers. Depending on the N source and the properties of the biochar, NH<sub>3</sub> loss reductions vary. Besides improving soil dynamics, BC can also be used in wastewater treatment. Engineered or designer biochar is positioned as a promising material for wastewater treatment due to its enhanced properties and versatility.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Migration of stable release high concentration toluene vapor and microbial activity varies in silty sand soil
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-02-18 DOI: 10.1007/s10532-025-10111-x
Zhongping Sun, Yaju Gong, Wenxia Wei, Yun Song
{"title":"Migration of stable release high concentration toluene vapor and microbial activity varies in silty sand soil","authors":"Zhongping Sun,&nbsp;Yaju Gong,&nbsp;Wenxia Wei,&nbsp;Yun Song","doi":"10.1007/s10532-025-10111-x","DOIUrl":"10.1007/s10532-025-10111-x","url":null,"abstract":"<div><p>Vapor intrusion (VI) happens when volatile organic compounds (VOCs) migrate from subsurface sources into buildings, harming indoor air quality and occupants’ health. To investigate the migration and biodegradation of volatile organic compounds (VOCs) originating from subsurface sources, a soil column experiment was performed. In this experiment, high-concentration vapor from the liquid phase of toluene was steadily released into silty sand soil, aiming to simulate the conceptual model of a specific site. The experimental findings revealed that, within the silty sand soil, it took 36 h for toluene vapors to diffuse through a 120-cm-long soil column. During this process, the volume fractions of O₂ and CO₂ within the soil column varied. The level of microbial activity in the soil column first rose and then declined, as did the abundance of the dominant degrading toluene bacteria group. The experiment demonstrated that covering a certain thickness of silty sand soil could effectively retard the migration of toluene vapor. In addition, biodegradation occurred during the migration of the toluene vapor. However, long-term exposure to high-concentration toluene vapors inhibited both the activity and growth of microorganisms within the soil column.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome analysis of a newly isolated Lysinibacillus fusiformis–YC01 for biodegrading inosine and guanosine
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-02-18 DOI: 10.1007/s10532-025-10117-5
Yu Zhang, Xiaoyu Cao, Jingyuan Cai, Meijie Song, Xinyue Du, Yang Liu, Qianqian Xu, Hai Yan
{"title":"Genome analysis of a newly isolated Lysinibacillus fusiformis–YC01 for biodegrading inosine and guanosine","authors":"Yu Zhang,&nbsp;Xiaoyu Cao,&nbsp;Jingyuan Cai,&nbsp;Meijie Song,&nbsp;Xinyue Du,&nbsp;Yang Liu,&nbsp;Qianqian Xu,&nbsp;Hai Yan","doi":"10.1007/s10532-025-10117-5","DOIUrl":"10.1007/s10532-025-10117-5","url":null,"abstract":"<div><p>Hyperuricemia (HUA) caused by high serum uric acid (UA) level can lead to a range of metabolic diseases, such as gout, cardiovascular disease and diabetes. The reduction of crucial UA precursors of both inosine and guanosine is a potential method to control HUA. Here a promising bacterial strain for biodegrading both inosine and guanosine were successfully isolated from <i>Baijiu</i> cellar mud and identified as <i>Lysinibacillus fusiformis</i>-YC01 by ANI analysis. Initial 490 mg/L of inosine and 612 mg/L of guanosine were completely biodegraded by YC01 within 18 h at 38 °C. In addition, the initial 357 mg/L of inosine and 365 mg/L of guanosine were also removed by the cell-free extracts of YC01 at a protein concentration of 0.13 mg/mL within 16 h. Furthermore, the whole genome analysis of YC01 revealed that purine nucleoside phosphorylase and purine nucleosidase played key roles in the biodegradation of inosine and guanosine, which encoded by gene <i>deoD</i> and gene <i>iunH</i>. These findings indicated that YC01 could biodegrade inosine and guanosine, and provided the new valuable insights into microbial removal of UA precursors for the amelioration of HUA.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combatting pesticide pollution: using liquid scintillation spectrometry to assess 14C-labeled hexachlorobenzene removal by mangrove Bacillus spp.
IF 3.1 4区 生物学
Biodegradation Pub Date : 2025-02-07 DOI: 10.1007/s10532-025-10113-9
Wanessa J. Santana Mota, Jessica R. de Jesus, Ana P. Justiniano Rego, Ayslan S. P. da Costa, Valdemar L. Tornisielo, Katlin I. Barrios Eguiluz, Giancarlo R. Salazar-Banda, Maria L. Hernández-Macedo, Jorge A. López
{"title":"Combatting pesticide pollution: using liquid scintillation spectrometry to assess 14C-labeled hexachlorobenzene removal by mangrove Bacillus spp.","authors":"Wanessa J. Santana Mota,&nbsp;Jessica R. de Jesus,&nbsp;Ana P. Justiniano Rego,&nbsp;Ayslan S. P. da Costa,&nbsp;Valdemar L. Tornisielo,&nbsp;Katlin I. Barrios Eguiluz,&nbsp;Giancarlo R. Salazar-Banda,&nbsp;Maria L. Hernández-Macedo,&nbsp;Jorge A. López","doi":"10.1007/s10532-025-10113-9","DOIUrl":"10.1007/s10532-025-10113-9","url":null,"abstract":"<div><p>This study explored the ability of two <i>Bacillus</i> species isolated from mangrove sediments to degrade hexachlorobenzene (HCB), a persistent organic pollutant that affects the quality of surface water, groundwater, and soil. Hence, we analyzed bacterial growth in a medium with hexachlorobenzene as the sole carbon source. Moreover, chemical oxygen demand removal, ecotoxicity, and measured radiolabeled HCB degradation were assessed. Our results revealed that both <i>Bacillus</i> strains (I3 and I6) demonstrated hexachlorobenzene-degrading potential and achieved degradation rates of 11.5 ± 1.47% and 21.1 ± 0.84%. Additionally, the ability of these strains to mineralize HCB was confirmed by the production of radiolabeled carbon dioxide, assessed by liquid scintillation spectrometry and thin-layer chromatography. Ecotoxicity assays further demonstrated the effectiveness of bacteria treatment in degrading HCB. These findings underscore the potential of <i>Bacillus</i> strains from mangrove sediments to degrade and mineralize HCB, opening new perspectives for the bioremediation of aromatic compounds in contaminated environments.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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