Biodegradation最新文献

{"title":"Biodegradation of 1,4-dioxane by a native digestate microbial community under different electron accepting conditions","authors":"Aryan Samadi,&nbsp;Azadeh Kermanshahi-pour,&nbsp;Suzanne M. Budge,&nbsp;Yannan Huang,&nbsp;Rob Jamieson","doi":"10.1007/s10532-023-10019-4","DOIUrl":"10.1007/s10532-023-10019-4","url":null,"abstract":"<div><p>The potential of a native digestate microbial community for 1,4-dioxane (DX) biodegradation was evaluated under low dissolved oxygen (DO) concentrations (1–3 mg/L) under different conditions in terms of electron acceptors, co-substrates, co-contaminants and temperature. Complete DX biodegradation (detection limit of 0.01 mg/L) of initial 25 mg/L was achieved in 119 days under low DO concentrations, while complete biodegradation happened faster at 91 and 77 days, respectively in nitrate-amended and aerated conditions. In addition, conducting biodegradation at 30 ˚C showed that the time required for complete DX biodegradation in unamended flasks reduced from 119 days in ambient condition (20–25 °C) to 84 days. Oxalic acid, which is a common metabolite of DX biodegradation was identified in the flasks under different treatments including unamended, nitrate-amended and aerated conditions. Furthermore, transition of the microbial community was monitored during the DX biodegradation period. While the overall richness and diversity of the microbial community decreased, several families of known DX-degrading bacteria such as <i>Pseudonocardiaceae</i>, <i>Xanthobacteraceae</i> and <i>Chitinophagaceae</i> were able to maintain and grow in different electron-accepting conditions. The results suggested that DX biodegradation under low DO concentrations, where no external aeration was provided, is possible by the digestate microbial community, which can be helpful to the ongoing research for DX bioremediation and natural attenuation.</p><h3>Graphical abstract</h3><figure><div><div><div><picture><source><img></source></picture></div></div></div></figure></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 3","pages":"283 - 300"},"PeriodicalIF":3.6,"publicationDate":"2023-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-023-10019-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4717849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the suitability of granular anammox biomass for nitrogen removal from vegetable tannery wastewater","authors":"C. Polizzi,&nbsp;T. Lotti,&nbsp;A. Ricoveri,&nbsp;G. Mori,&nbsp;D. Gabriel,&nbsp;G. Munz","doi":"10.1007/s10532-023-10017-6","DOIUrl":"10.1007/s10532-023-10017-6","url":null,"abstract":"<div><p>In the present study, the potential inhibitory effect of biologically pre-treated vegetable tannery wastewater (TW) on anammox granular biomass was evaluated. Beside high organic and chemicals load, vegetable TW are characterised by high salinity and high tannins concentration, the latter belonging to a group of bio-refractory organic compounds, potentially inhibitory for several bacterial species. Recalcitrant tannin-related organic matters and salinity were selected as the two potential inhibitory factors and studied either for their separate and combined effect. Parallel batch tests were performed, with biomass acclimated and non-acclimated to salinity, testing three different conditions: non-saline control test with non-acclimated biomass (CT); saline control test with acclimated biomass (SCT); vegetable tannery wastewater test with acclimated biomass (TWT). Compared with non-saline CT, the specific anammox activity in tests SCT and TWT showed a reduction of 28 and 14%, respectively, suggesting that salinity, at conductivity values of 10 mS/cm (at 25 °C), was the main impacting parameter. As a general conclusion, the study reveals that there is no technical limitation for the application of the anammox process to vegetable TW, but preliminary biomass acclimation as well as regular biomass activity monitoring is recommended in case of long-term applications. To the best of our knowledge, this is the first work assessing the impact of vegetable TW on anammox biomass.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 3","pages":"253 - 262"},"PeriodicalIF":3.6,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-023-10017-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4971191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metaproteomic and gene expression analysis of interspecies interactions in a PAH-degrading synthetic microbial consortium constructed with the key microbes of a natural consortium","authors":"Esteban E. Nieto,&nbsp;Marianela Macchi,&nbsp;María P. Valacco,&nbsp;Sabrina Festa,&nbsp;Irma S. Morelli,&nbsp;Bibiana M. Coppotelli","doi":"10.1007/s10532-022-10012-3","DOIUrl":"10.1007/s10532-022-10012-3","url":null,"abstract":"<div><p>Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (<i>Sphingobium</i> sp. AM and <i>Burkholderia</i> sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, <i>naph, biph, tol</i> and <i>sal</i> and the AM gene, <i>ahdB,</i> in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 2","pages":"181 - 197"},"PeriodicalIF":3.6,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4502893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of the molybdenum-resistant strain Raoultella ornithinolytica A1 and its effect on MoO42− in the environment","authors":"Jie Xing,&nbsp;Chunyan Li,&nbsp;Wanting Li,&nbsp;Xuemei Zhang,&nbsp;Zhaoquan Li,&nbsp;Ang Li","doi":"10.1007/s10532-022-10011-4","DOIUrl":"10.1007/s10532-022-10011-4","url":null,"abstract":"","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 2","pages":"169 - 180"},"PeriodicalIF":3.6,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4162421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of co-contaminants and dilution on perchlorate biodegradation using various carbon sources","authors":"Yasaman Saedi,&nbsp;Jacimaria R. Batista,&nbsp;Ronnie Britto,&nbsp;Dana Grady","doi":"10.1007/s10532-022-10013-2","DOIUrl":"10.1007/s10532-022-10013-2","url":null,"abstract":"<div><p>This research investigates the biodegradation of perchlorate in the presence of the co-contaminants nitrate and chlorate using soluble and slow-release carbon sources. In addition, the impact of bio-augmentation and dilution, which results in lower total dissolved salts (TDS) and contaminant levels, is examined. Laboratory microcosms were conducted using actual groundwater and soils from a contaminated aquifer. The results revealed that both soluble and slow-release carbon sources support biodegradation of contaminants in the sequence nitrate &gt; chlorate &gt; perchlorate. Degradation rates, including and excluding lag times, revealed that the overall impact of the presence of co-contaminants depends on degradation kinetics and the relative concentrations of the contaminants. When the lag time caused by the presence of the co-contaminants is considered, the degradation rates for chlorate and perchlorate were two to three times slower. The results also show that dilution causes lower initial contaminant concentrations, and consequently, slower degradation rates, which is not desirable. On the other hand, the dilution resulting from the injection of amendments to support remediation promotes desirably lower salinity levels. However, the salinity associated with the presence of sulfate does not inhibit biodegradation. The naturally occurring bacteria were able to support the degradation of all contaminants. Bio-augmentation was effective only in diluted microcosms. <i>Proteobacteria</i> and <i>Firmicutes</i> were the dominant phyla identified in the microcosms.</p><h3>Graphical abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 4","pages":"301 - 323"},"PeriodicalIF":3.6,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-022-10013-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4164180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of organic compounds in the coal gangue by Bacillus sp. into humic acid","authors":"Chenxu Liu,&nbsp;Shuhua Ma,&nbsp;Xiaohui Wang,&nbsp;Yanjun Ou,&nbsp;Hao Du","doi":"10.1007/s10532-022-10007-0","DOIUrl":"10.1007/s10532-022-10007-0","url":null,"abstract":"<div><p>Coal gangue (CG), one of the world’s largest industrial solid wastes produced during coal mining, is extremely difficult to be used owing to its combined contents of clay minerals and organic macromolecules. This study explored a novel process of degrading the harmful organic compounds in the CG into humic acid using a biological method characterized by scanning electron microscope–energy dispersive spectrometer, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and elemental analyzer. The results reveal that adding selected <i>Bacillus</i> sp. to the CG for 40 days can increase the humic acid content by ~ 17 times, reaching 17338.17 mg/kg, which is also the best level for promoting plant growth. FTIR and XPS spectra show that the organic compounds in the CG transforms primarily from C=C to C=O, COOH, and O–H groups, indicating that the organic compounds are gradually oxidized and activated, improving the humic acid concentration of soil. In addition, <i>Bacillus</i> sp. decreases pH and benzo[a]pyrene contents, and increases the content of available nutrients. After microbial degradation, coal gangue can be turned into ecological restoration materials.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 2","pages":"125 - 138"},"PeriodicalIF":3.6,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-022-10007-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4467880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of chemicals tested in mixtures and individually: mixture effects on biodegradation kinetics and microbial composition","authors":"Heidi Birch,&nbsp;Arnaud Dechesne,&nbsp;Karina Knudsmark Sjøholm,&nbsp;Philipp Mayer","doi":"10.1007/s10532-022-10009-y","DOIUrl":"10.1007/s10532-022-10009-y","url":null,"abstract":"<div><p>Biodegradation in the aquatic environment occurs in the presence of many chemicals, while standard simulation biodegradation tests are conducted with single chemicals. This study aimed to investigate the effect of the presence of additional chemicals on (1) biodegradation kinetics of individual chemicals and (2) the microbial composition in test systems. Parallel mixture and single substance experiments were conducted for 9 chemicals (phenethyl benzoate, oxacycloheptadec-10-en-2-one, α-ionone, methyl 2-naphthyl ether, decan-5-olide, octan-2-one, 2′-acetonaphthanone, methyl <i>N</i>-methylanthranilate, (+)-menthone) using inoculum from a Danish stream. Biotic and abiotic test systems were incubated at 12 °C for 1–30 days. Primary biodegradation kinetics were then determined from biotic/abiotic peak area ratios using SPME GC/MS analysis. The effect of the mixture on biodegradation varied with test chemical and was more pronounced for chemicals with lag-phases above 14 days: two chemicals degraded in the mixture but not when tested alone (i.e., positive mixture effect), and two degraded when tested alone but not in the mixture (i.e., negative mixture effect). Microbial composition (16S rRNA gene amplicon sequencing) was highly affected by 14 days incubation and the presence of the mixture (significant carbon source), but less by single chemicals (low carbon source). Growth on chemical mixtures resulted in consistent proliferation of <i>Pseudomonas</i> and <i>Malikia</i>, while specific chemicals increased the abundance of putative degraders belonging to <i>Novosphingobium</i> and <i>Zoogloea.</i> The chemical and microbiological results support (1) that simulation biodegradation kinetics should be determined in mixtures at low environmentally relevant concentrations and (2) that degradation times beyond some weeks are associated with more uncertainty.</p><h3>Graphical abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 2","pages":"139 - 153"},"PeriodicalIF":3.6,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4121295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced bioremediation of oil-contaminated soil in a slurry bioreactor by H2O2-stimulation of oil-degrading/biosurfactant-generating bacteria: performance optimization and bacterial metagenomics","authors":"Farzaneh Fanaei,&nbsp;Gholamreza Moussavi,&nbsp;Sakine Shekoohiyan","doi":"10.1007/s10532-022-10008-z","DOIUrl":"10.1007/s10532-022-10008-z","url":null,"abstract":"<div><p>Oil-contaminated soil is the main challenge for oil-rich countries, and this study aimed to investigate the performance of the H₂O₂-stimulated slurry bioreactor for the bioremediation of real oil-contaminated soil. The effect of biomass concentration, soil to water (S/W) ratio, slurry temperature, pH, and H₂O₂ concentration were optimized for the removal of total petroleum hydrocarbons (TPH) from oil-contaminated soil. TPH removal efficiency, biosurfactants production, and peroxidase and dehydrogenase activities were measured. The optimum conditions for the complete biodegradation of 32 <span>({text{g}}_{{{text{TPH}}}} /{text{kg}}_{{{text{soil}}}})</span> in the slurry bioreactor during 6 days were biomass of 2250 mg/L, S/W ratio of 20%, the temperature of 30 °C, pH of 7, and an H₂O₂ concentration of 120 mg/L. The highest peroxidase, dehydrogenase, surfactin, and rhamnolipid formation were also obtained under optimum conditions. The results pointed out that complete biodegradation of 32 g/kg of TPH in oil-contaminated soil at a short reaction time of 6 days is achievable in the developed process operated under optimum conditions. The GC/FID analysis of solid and liquid phases showed that the bioprocess completely biodegraded the different TPH fractions. H₂O₂ efficiently stimulated the biosurfactant-generating bacteria to produce peroxidase and thereby accelerating the bioremediation rate. Accordingly, an H₂O₂-mediated slurry bioreactor inoculated with biosurfactant/peroxidase-generating bacteria is a promising technique for cleaning up oil-contaminated soils.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 1","pages":"83 - 101"},"PeriodicalIF":3.6,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4083803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Goethite and riboflavin synergistically enhance Cr(VI) reduction by Shewanella oneidensis MR-1","authors":"Yaqin Zheng,&nbsp;Haibo Li,&nbsp;Yue Yang,&nbsp;Bingze Wu,&nbsp;Xinjing Li,&nbsp;Kaixuan Wang,&nbsp;Pengkai Wang,&nbsp;Chenxi Zhang","doi":"10.1007/s10532-022-10010-5","DOIUrl":"10.1007/s10532-022-10010-5","url":null,"abstract":"<div><p>Bioreduction of Cr(VI) is cost-effective and environmentally friendly, however, the slow bioreduction rate limits its application. In this study, the potential synergistic enhancement of Cr(VI) bioreduction by <i>shewanella oneidensis</i> MR-1 (<i>S. oneidensis</i>) with goethite and riboflavin (RF) was investigated. The results showed that the <i>S. oneidensis</i> reaction system reduce 29.2% of 20 mg/L Cr(VI) after 42 h reaction, while the <i>S. oneidensis</i>/goethite/RF reaction system increased the Cr(VI) reduction rate to 87.74%. RF as an efficient electron shuttle and Fe(II) from goethite bioreduction were identified as the crucial components in Cr(VI) reduction. XPS analysis showed that the final precipitates of Cr(VI) reduction were Cr(CH₃C(O)CHC(O)CH₃)₃ and Cr₂O₃ and adhered to the bacterial cell surface. In this process, the microbial surface functional groups such as hydroxyl and carboxyl groups participated in the adsorption and reduction of Cr(VI). Meanwhile, an increase in cytochrome c led to an increase in electron transfer system activity (ETSA), causing a significant enhancement in extracellular electron transfer efficiency. This study provides insight into the mechanism of Cr(VI) reduction in a complex environment where microorganisms, iron minerals and RF coexist, and the synergistic treatment method of Fe(III) minerals and RF has great potential application for Cr(VI) detoxification in aqueous environment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 2","pages":"155 - 167"},"PeriodicalIF":3.6,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4084092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of heavy oily sludge: a microcosms study","authors":"Cinthya Rondon-Afanador,&nbsp;Gustavo Pinilla-Meza,&nbsp;Francy C. Casallas-Cuervo,&nbsp;Camila Diaz-Vanegas,&nbsp;Daniela Barreto-Gomez,&nbsp;Carolina Benavides,&nbsp;Nicole Buitrago,&nbsp;Melissa Calvo,&nbsp;Camila Forero-Forero,&nbsp;Valentina Galvis-Ibarra,&nbsp;Victoria Moscoso-Urdaneta,&nbsp;Maria C. Perdomo-Rengifo,&nbsp;Laura Torres,&nbsp;Ziv Arbeli,&nbsp;Robin L. Brigmon,&nbsp;Fabio Roldan","doi":"10.1007/s10532-022-10006-1","DOIUrl":"10.1007/s10532-022-10006-1","url":null,"abstract":"<div><p>Oily sludge is a residue from the petroleum industry composed of a mixture of sand, water, metals, and high content of hydrocarbons (HCs). The heavy oily sludge used in this study originated from Colombian crude oil with high density and low American Petroleum Institute (API) gravity. The residual waste from heavy oil processing was subject to thermal and centrifugal extraction, resulting in heavy oily sludge with very high density and viscosity. Biodegradation of the total petroleum hydrocarbons (TPH) was tested in microcosms using several bioremediation approaches, including: biostimulation with bulking agents and nutrients, the surfactant Tween 80, and bioaugmentation. Select HC degrading bacteria were isolated based on their ability to grow and produce clear zones on different HCs. Degradation of TPH in the microcosms was monitored gravimetrically and with gas chromatography (GC). The TPH removal in all treatments ranged between 2 and 67%, regardless of the addition of microbial consortiums, amendments, or surfactants within the tested parameters. The results of this study demonstrated that bioremediation of heavy oily sludge presents greater challenges to achieve regulatory requirements. Additional physicochemical treatments analysis to remediate this recalcitrant material may be required to achieve a desirable degradation rate.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 1","pages":"1 - 20"},"PeriodicalIF":3.6,"publicationDate":"2022-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-022-10006-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4155776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}