Biodegradation最新文献_第10页

Synergistic application of biochar and mercury-resistant Bacillus cereus enhances phytoremediation efficiency and stress tolerance in sorghum (Sorghum bicolor L.) grown in mercury-contaminated soil 生物炭与耐汞蜡样芽孢杆菌协同应用提高了汞污染土壤中高粱的植物修复效率和抗逆性。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-30 DOI: 10.1007/s10532-026-10294-x

Ghulam Murtaza, Muhammad Usman, Zeeshan Ahmed, Rashid Iqbal

{"title":"Synergistic application of biochar and mercury-resistant Bacillus cereus enhances phytoremediation efficiency and stress tolerance in sorghum (Sorghum bicolor L.) grown in mercury-contaminated soil","authors":"Ghulam Murtaza, Muhammad Usman, Zeeshan Ahmed, Rashid Iqbal","doi":"10.1007/s10532-026-10294-x","DOIUrl":"10.1007/s10532-026-10294-x","url":null,"abstract":"<div><p>Mercury (Hg) contamination in agricultural soils threatens crop productivity and ecosystem sustainability. This study investigates the synergistic effects of <i>Pieris japonica</i>-derived biochar and the Hg-resistant bacterium <i>Bacillus cereus</i> B2 in mitigating Hg toxicity in sorghum (<i>Sorghum bicolor</i> L.) grown in Hg-contaminated soils (0, 15, 20, and 25 mg Hg kg⁻<sup>1</sup>). Hg stress significantly inhibited plant growth, gas exchange, and photosynthetic pigments, with the greatest reductions at 25 mg kg⁻<sup>1</sup> Hg. The combined application of biochar (4%) and <i>B. cereus</i> B2 notably enhanced plant height (34.7%), stomatal conductance (50.1%), photosynthetic rate (24.5%), transpiration rate (60.2%), water-use efficiency (24.5%), chlorophyll a (75.8%), chlorophyll b (29.3%), carotenoids (27.0%), and total chlorophyll (59.5%) compared to individual treatments. This synergistic approach also stimulated antioxidant defenses, increasing activities of ascorbate peroxidase (20.1%), catalase (25.2%), superoxide dismutase (34.9%), and peroxidase (49.7%), while reducing reactive oxygen species (H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub>⁻) and lipid peroxidation (MDA). Furthermore, Hg accumulation in sorghum roots and shoots was significantly reduced under the combined treatment, indicating lower Hg bioavailability. These findings demonstrate the novel synergistic potential of <i>P. japonica</i> biochar and <i>B. cereus</i> B2 in enhancing Hg immobilization, improving plant physiological resilience, and strengthening antioxidant defenses in sorghum, providing an effective and sustainable strategy for Hg-contaminated soil remediation.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the catalytic potential of a Cycloclasticus zancles ring-hydroxylating dioxygenase through computational analysis for enhanced PAH biodegradation 通过计算分析解码环虫环羟化双加氧酶对多环芳烃生物降解的催化潜力。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-28 DOI: 10.1007/s10532-026-10298-7

Muhammad Naveed, Ahmed Raza, Ahiba Adil, Abubakar Majeed, Sana Miraj Khan, Ayaz Ali Khan, Rania Ali El Hadi Mohamed, Nawal H. Siddig, Hayam A. Alwabsi, Hanan Abdulrahman Sagini

{"title":"Decoding the catalytic potential of a Cycloclasticus zancles ring-hydroxylating dioxygenase through computational analysis for enhanced PAH biodegradation","authors":"Muhammad Naveed, Ahmed Raza, Ahiba Adil, Abubakar Majeed, Sana Miraj Khan, Ayaz Ali Khan, Rania Ali El Hadi Mohamed, Nawal H. Siddig, Hayam A. Alwabsi, Hanan Abdulrahman Sagini","doi":"10.1007/s10532-026-10298-7","DOIUrl":"10.1007/s10532-026-10298-7","url":null,"abstract":"<div><p>Polycyclic aromatic hydrocarbons (PAHs) are widespread, toxic, and recalcitrant pollutants in marine ecosystems. Microbial biodegradation offers a sustainable remediation strategy, with <i>Cycloclasticus zancles</i> recognized as a keystone degrader. Here, we used in silico approaches to characterize a ring-hydroxylating dioxygenase (RHD), the enzyme catalyzing the initial and rate-limiting step of PAH catabolism. The AlphaFold-predicted RHD structure was validated using PROCHECK and ERRAT, confirming strong stereochemical and structural reliability. Toxicity screening classified all twenty PAHs as Cramer Class III, underscoring the ecological risk and the need for effective degradation. Protein–protein interaction and co-expression analyses revealed associations with electron transfer partners and conserved dioxygenase-related genes, suggesting coordinated regulation of hydrocarbon metabolism. Molecular docking showed high binding affinities for large PAHs, with benzo[a]pyrene (− 9.3 kcal/mol), benzo[b]fluoranthene (− 9.1 kcal/mol), and benzo[e]pyrene (− 9.0 kcal/mol) forming the most stable complexes. Interaction profiling highlighted stabilization through hydrophobic contacts, π–π stacking, and hydrogen bonding with residues including Phe223, Tyr276, Leu278, Asn252, and Gln309. Collectively, these results demonstrate the enzyme’s catalytic adaptability, biosafety relevance, and ecological significance, establishing <i>C. zancles</i> RHD as a promising candidate biocatalyst for marine PAH bioremediation.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic pretreatment: hybrid strategies for maximum lignocellulose valorization 协同预处理：最大木质纤维素增值的杂交策略

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-22 DOI: 10.1007/s10532-026-10296-9

Nikunj Mehta

{"title":"Synergistic pretreatment: hybrid strategies for maximum lignocellulose valorization","authors":"Nikunj Mehta","doi":"10.1007/s10532-026-10296-9","DOIUrl":"10.1007/s10532-026-10296-9","url":null,"abstract":"<div><p>Lignocellulosic biomass (LCB) is a vast, renewable resource critical to a circular bioeconomy, but its inherent recalcitrance remains the principal barrier to efficient enzymatic saccharification and valorization. Given the numerous existing reviews that simply catalogue individual pretreatment methods, the necessity of this review lies in its critical evaluation of how hybridizing standalone technologies is essential to overcome current pilot-scale and commercialization bottlenecks. This review provides a comparative analysis of three emerging pretreatment technologies: hydrothermal (HTP), microwave-assisted (MWP), and ball milling (BM). The author analyzes the distinct mechanisms by which each technology decreases the recalcitrance of LCB. HTP excels at hemicellulose hydrolysis via autohydrolysis but is plagued by the formation of inhibitors and pseudo-lignin. MWP employs rapid dielectric heating to achieve similar objectives within minutes, compared with the hours often required for conventional HTP, demonstrating high energy efficiency (e.g., 40.1 kJ/g compared to conventional HTP at 70.85 kJ/g), but faces fundamental commercial scale-up challenges related to finite penetration depths and hotspots. BM, a mechanochemical approach, is unparalleled in destroying cellulose crystallinity, dramatically enhancing kinetics without producing inhibitors, but it suffers from prohibitively high energy consumption, often requiring up to 2.8 kWh/kg. The author concludes that commercial viability dictates a trend toward hybrid, synergistic processes, such as BM-HTP and MW-HTP, which balance trade-offs and achieve near-theoretical glucose yields of 97.3%. Future research must focus on continuous-flow reactor engineering, integration with lignin-first valorization strategies, and predictive AI/ML modeling to enable economically competitive lignocellulosic biorefineries.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-026-10296-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhamnolipid-like glycolipid biosurfactant mediated degradation of phenanthrene and fluoranthene by Pseudomonas aeruginosa and its impact on soil enzyme activities 鼠李糖脂样糖脂生物表面活性剂介导的铜绿假单胞菌对菲和氟蒽的降解及其对土壤酶活性的影响

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-18 DOI: 10.1007/s10532-026-10297-8

Bhrigu Bhuyan, Ankita Das, Riya Chakraborty, Piyush Pandey

{"title":"Rhamnolipid-like glycolipid biosurfactant mediated degradation of phenanthrene and fluoranthene by Pseudomonas aeruginosa and its impact on soil enzyme activities","authors":"Bhrigu Bhuyan, Ankita Das, Riya Chakraborty, Piyush Pandey","doi":"10.1007/s10532-026-10297-8","DOIUrl":"10.1007/s10532-026-10297-8","url":null,"abstract":"<div><p>Phenanthrene (Phe) and fluoranthene (Fla) are polycyclic aromatic hydrocarbons (PAHs) known for their significant toxicity and resistance to degradation, making them biohazardous substances. This study explains the efficient degradation of Phe and Fla using two bacterial isolates—<i>Pseudomonas aeruginosa</i> BB-BE3, and <i>P. aeruginosa</i> BBBJ. These isolates produced excellent amount of rhamnolipid-like glycolipid biosurfactant, with strong emulsification activity. The isolate BB-BE3 attained degradation rates of up to 93% for Phe and 73% for Fla, while BBBJ achieved degradation rate of 81% for Phe and 76% for Fla, respectively under in vitro conditions. Both isolates were found to produce catechol 1,2 dioxygenase and catechol 2,3 dioxygenase enzymes. In microcosm study, treatment of PAH-contaminated soil with biosurfactant-producing bacterial isolates in combination with their extracted biosurfactants resulted in substantially enhanced degradation efficiencies, achieving 89–99% PAHs removal across all experimental setups compared to the control. Soil enzyme activities, including dehydrogenase and fluorescein diacetate hydrolysis, were evaluated as indicators of microbial metabolic activity. Notably, soils inoculated with biosurfactant-producing bacteria along with extracted biosurfactants exhibited elevated enzyme activities at both 15 and 30 days of incubation, reflecting sustained and robust microbial activity. Collectively, these findings demonstrate the strong potential of biosurfactant-producing bacterial isolates as an effective bioremediation strategy for the degradation of PAHs in contaminated soils.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative performance of non-white rot fungal stirred-tank and biofilm-based bioreactors for pharmaceutical wastewater treatment: kinetic modelling and scalability potential 非白腐真菌搅拌槽和生物膜生物反应器在制药废水处理中的比较性能：动力学建模和可扩展性潜力。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-16 DOI: 10.1007/s10532-026-10295-w

Ibtihal Alkarim, Nur Aliya Narzilan, Nur Syahidah Shaharuddin, Murni Halim, Mohd Shamzi Mohamed, Fadzlie Wong Faizal Wong

{"title":"Comparative performance of non-white rot fungal stirred-tank and biofilm-based bioreactors for pharmaceutical wastewater treatment: kinetic modelling and scalability potential","authors":"Ibtihal Alkarim, Nur Aliya Narzilan, Nur Syahidah Shaharuddin, Murni Halim, Mohd Shamzi Mohamed, Fadzlie Wong Faizal Wong","doi":"10.1007/s10532-026-10295-w","DOIUrl":"10.1007/s10532-026-10295-w","url":null,"abstract":"<div><p>Pharmaceutically active compound (PhAC) is poorly removed by conventional wastewater treatment, threatening aquatic ecosystems and human health through persistent bioaccumulation. To address this challenge, we evaluated two underutilized non-white rot fungi, <i>Aspergillus ele</i>and <i>Cunninghamella elegans</i>, in stirred-tank (STBR) and biofilm-based (BBR) bioreactors for removing fluoxetine (FLX), ciprofloxacin (CIP), and atorvastatin (ATO). <i>A. flavus</i> in STBR achieved exceptional removal (92–94% for all PhACs), with both STBR and BBR achieving statistically comparable pharmaceutical removal efficiencies (p > 0.05). While STBR excelled in targeted PhAC degradation, BBR better improved general wastewater quality, reducing chemical oxygen demand (COD) by 73.7% and total organic carbon (TOC) by 52.6%. Surprisingly, laccase activity showed no correlation with degradation, suggesting that intracellular enzymatic mechanisms may be responsible; cytochrome P450-mediated biotransformation is proposed as a hypothesis warranting future experimental validation. These results demonstrate that non-white rot fungi offer promising, energy-efficient alternatives for pharmaceutical wastewater treatment, with bioreactor choice dependent on prioritization of specific pollutant removal versus broad effluent polishing.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green biocatalysis: Box–Behnken-optimized cellulase from thermophilic Streptomyces griseorubens NBR14 for waste management and dye removal 绿色生物催化：box - behnken优化的纤维素酶从嗜热的灰色链霉菌NBR14用于废物管理和染料去除。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-15 DOI: 10.1007/s10532-026-10287-w

Abd El-Rahman Fathy Gomaa, Sheng Qin, Mohamed H. El-Sayed, Asmaa M. M. Mawad

{"title":"Green biocatalysis: Box–Behnken-optimized cellulase from thermophilic Streptomyces griseorubens NBR14 for waste management and dye removal","authors":"Abd El-Rahman Fathy Gomaa, Sheng Qin, Mohamed H. El-Sayed, Asmaa M. M. Mawad","doi":"10.1007/s10532-026-10287-w","DOIUrl":"10.1007/s10532-026-10287-w","url":null,"abstract":"<div><p>Thermotolerant actinobacteria are important because they thrive in harsh environments and efficiently degrade agricultural residues, producing valuable enzymes for various applications. In this study, a thermophilic <i>Streptomyces griseorubens</i> NBR14 strain was used to produce cellulase from wheat straw via submerged fermentation. The production was optimized using a Box–Behnken Design (BBD). The enzyme was partially purified with acetone, followed by an analysis of its properties and potential use in dye removal. The results indicated that the enzyme yield increased 2.48-fold using this design under the optimized parameters 30 °C, pH 7.0 and 3% wheat straw after 5 days of incubation. The partially purified cellulase was characterized by 3.48-fold purification, with a specific activity of 158.22 U/mg. The optimal temperature and pH for cellulase activity were 50 °C and 7.0, respectively. It remained 96% at pH 9.0 and 73% active at 80 °C, respectively. The enzyme exhibited high activity (125%) and stability at 7% NaCl. Its activity was enhanced by 5% of tween 20 and 5 mM of sodium dodecyl sulfate (SDS) and sodium lauryl sulfate (SLS). The kinetic analysis of the partially purified cellulase showed that Km and Vmax were 0.74 mmol and 20.92 U/mL, respectively. The partially purified enzyme removed 83.6% of 50 mg/L of crystal violet in a quiescent state after 24 h of incubation. The Cellulase was immobilized in calcium alginate beads, resulting in an immobilization yield of 75.07 ± 1.35%, which suggested a considerable potential for reuse. Therefore, cellulase from the thermophilic NBR14 enzyme is a promising biotechnological candidate for cellulosic waste management and a valuable tool for industrial monitoring under extreme conditions.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichoderma harzianum enhances lettuce biomass and modulates plant-soil emerging contaminant dynamics under reclaimed wastewater irrigation 再生废水灌溉下哈兹木霉提高生菜生物量并调节植物-土壤新污染物动态。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-15 DOI: 10.1007/s10532-026-10291-0

Monica Brienza, Juan Manuel Peña-Herrera, Vincenzo Trotta, Serge Chiron, Andrés Sauvêtre

{"title":"Trichoderma harzianum enhances lettuce biomass and modulates plant-soil emerging contaminant dynamics under reclaimed wastewater irrigation","authors":"Monica Brienza, Juan Manuel Peña-Herrera, Vincenzo Trotta, Serge Chiron, Andrés Sauvêtre","doi":"10.1007/s10532-026-10291-0","DOIUrl":"10.1007/s10532-026-10291-0","url":null,"abstract":"<div><p>The use of wastewater for irrigation in agricultural soils offers a sustainable means to reduce freshwater consumption and recycle nutrients, but also poses contamination risks associated with emerging pollutants. Furthermore, potentially toxic transformation products may form in soil and rhizosphere, and could subsequently be taken up by plants. Several products containing spores of symbiotic fungi are available and could serve as effective solutions to enhance the biodegradation of micropollutants in agricultural soils irrigated with treated wastewater. In this study, the effect of the fungus <i>Trichoderma harzianum</i> on the distribution of two emerging contaminants, carbamazepine and climbazole, and their main transformation products among soil and lettuce tissues was investigated under controlled conditions. Physiological effects were also investigated by quantifying phytohormones in roots and leaves. Inoculation with <i>Trichoderma harzianum</i> did not significantly affect the uptake of the parent compounds but increased the concentration of their transformation products in soil and reduced their levels in plant leaves after three weeks. The fungus enhanced plant biomass and altered certain phytohormones involved in defense mechanisms such as salicylic acid and microbiome recruitment in roots and soils. Findings of this study provide valuable insights that can be effectively applied to crop cultivation using reclaimed wastewater, leveraging readily available biological products for improved sustainable practices.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of arsenic contamination on plants and the role of microalgae in arsenic stress mitigation for sustainable agriculture 砷污染对植物的影响及微藻在可持续农业砷胁迫缓解中的作用

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-13 DOI: 10.1007/s10532-026-10293-y

Rajneesh Singh, Rachana Singh

{"title":"Impacts of arsenic contamination on plants and the role of microalgae in arsenic stress mitigation for sustainable agriculture","authors":"Rajneesh Singh, Rachana Singh","doi":"10.1007/s10532-026-10293-y","DOIUrl":"10.1007/s10532-026-10293-y","url":null,"abstract":"<div><p>Arsenic (As) is a toxic metalloid that is accumulating in agricultural soils and irrigation water. As is occurring from both natural geochemical processes and various anthropogenic activities. Consequently, arsenic contamination is a serious global concern, negatively affecting plant productivity, food safety, and human health. Plants absorb As from the soil through their roots via various transport mechanisms. The absorbed As is further translocated to the shoots and other tissues, where it involved in several metabolic activities and hinders in various physiological and biochemical processes within the plants. Microalgae-based bioremediation, known as phycoremediation, has emerging strategy for mitigating arsenic contamination in affected agricultural soil and water due to its eco-friendly nature, cost-effectiveness, and sustainable applicability. Several microalgal species possess high tolerance capacity, ease of cultivation, strong metal-binding affinity, large surface area, and potential for reuse. These microalgaes have evolved as natural bioremediators with multiple detoxification mechanisms, including biosorption, bioaccumulation, biotransformation, and biomineralization, which enable it to immobilize, transform, or sequester As from contaminated soil, and water. This review explores the relationship between As exposure, As impact on plants system and the role of microalgae as efficient phycoremediators. Furthermore, it focus on the strategies to mitigate the detrimental effects of As contamination through microalgae-based remediation, elucidating the underlying mechanisms of As-induced toxicity and detoxification.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal bioremediation of petroleum hydrocarbons in terrestrial environments: a systematic review and meta-analysis 陆地环境中石油烃的真菌生物修复：系统综述和荟萃分析

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-04 DOI: 10.1007/s10532-026-10292-z

Matias Soto-Mancilla, Facundo Fioroni, Luciana Ebrecht, Mara Martin, Ma. Cecilia Mestre, Natalia V. Fernández

{"title":"Fungal bioremediation of petroleum hydrocarbons in terrestrial environments: a systematic review and meta-analysis","authors":"Matias Soto-Mancilla, Facundo Fioroni, Luciana Ebrecht, Mara Martin, Ma. Cecilia Mestre, Natalia V. Fernández","doi":"10.1007/s10532-026-10292-z","DOIUrl":"10.1007/s10532-026-10292-z","url":null,"abstract":"<div><p>Petroleum hydrocarbon (PH) contamination in terrestrial environments is a serious global concern due to the toxicity and persistence of these compounds. Fungi are promising agents in bioremediation because of the ability to tolerate, degrade and metabolize complex and recalcitrant PHs. However, the use of other organisms, such as bacteria, remains more prevalent. Here, we evaluated the extent to which fungi have been investigated in PH bioremediation through a systematic review and meta-analysis following PRISMA guidelines. Among the 1,749 studies selected on bioremediation, only 348 included fungi, of which 97 met the inclusion criteria for quantitative analysis. Fungi were included in less studied (20% of articles) than bacteria and plants (63% and 31% respectively). However, our meta-analysis revealed that fungal treatments significantly reduced residual hydrocarbons by 50–60% in comparison with controls, with filamentous fungi (e.g., <i>Trametes</i>, <i>Peniophora</i>) showing the highest efficacy. Mycorrhizal fungi also demonstrated notable reduction (45–49%), while yeasts were less effective, particularly for polycyclic aromatic hydrocarbons (PAHs). We further identified that experimental variables, including treatment duration and the type of contaminated matrix, significantly influenced hydrocarbon reduction efficiency. Key limitations in the literature included a strong bias toward filamentous fungi and PAHs, as well as inconsistencies in experimental methodologies. Overall, our findings highlight the significant potential of fungi as bioremediation agents and emphasize the need to expand research on complex hydrocarbon mixtures (e.g., fuels) and underrepresented fungal groups, such as yeasts and mycorrhizal fungi, to better understand their role in reducing PH contamination in terrestrial ecosystems.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147606414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico evaluation of protocatechuate 4,5-dioxygenase for environmental detoxification of persistent organic pollutants 原儿茶酸4,5-双加氧酶对持久性有机污染物环境解毒作用的硅片评价。

IF 3.2 4区生物学

Biodegradation Pub Date : 2026-04-02 DOI: 10.1007/s10532-026-10284-z

Muhammad Naveed, Maryum Rasheed, Maida Salah Ud Din, Minahil Zahid, Ayaz Ali Khan, Mayasar I. Al Zaban, Ashwag Shami, Hayam A. Alwabsi

{"title":"In silico evaluation of protocatechuate 4,5-dioxygenase for environmental detoxification of persistent organic pollutants","authors":"Muhammad Naveed, Maryum Rasheed, Maida Salah Ud Din, Minahil Zahid, Ayaz Ali Khan, Mayasar I. Al Zaban, Ashwag Shami, Hayam A. Alwabsi","doi":"10.1007/s10532-026-10284-z","DOIUrl":"10.1007/s10532-026-10284-z","url":null,"abstract":"<div><p>Among the persistent environmental pollutants with well-established toxic, mutagenic, and carcinogenic properties are Phenolic compounds and polycyclic aromatic hydrocarbons (PAHs). This study applied an in-silico framework to evaluate the predicted binding affinity of <i>Sphingomonas paucimobilis</i> protocatechuate 4,5-dioxygenase (LigAB; PDB ID: 1BOU) against selected phenolic derivatives and polycyclic aromatic hydrocarbons. Functional annotation via QuickGO confirmed its oxidoreductase and dioxygenase roles in aromatic ring cleavage. Binding site prediction using PrankWeb identified nine putative catalytic pockets. Nineteen environmentally relevant pollutants were retrieved from PubChem and subjected to molecular docking with LigAB using PyRx. Among them, dibenzo[a,h]anthracene (− 8.8 kcal/mol), benzo[a]pyrene (− 8.2 kcal/mol), and chrysene (− 7.7 kcal/mol) exhibited the strongest affinities, stabilized by π–π stacking, π–alkyl, and hydrogen bonding interactions as revealed by Discovery Studio. Toxicity profiling through ProTox 3.0 indicated moderate acute toxicity (LD<sub>50</sub> = 316 mg/kg) for dibenzo[a,h]anthracene, alongside high hepatotoxic, mutagenic, and carcinogenic risks, underscoring the environmental urgency of its removal. Collectively, these findings highlight LigAB as a promising microbial biocatalyst for targeting recalcitrant polycyclic aromatic hydrocarbons and demonstrate the utility of computational pipelines for rapid enzyme–substrate screening in bioremediation research.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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