Biodegradation最新文献

{"title":"The role of green chemistry in the transformation of agro-industrial wastes for health and environment: a review","authors":"Manoj Chandra Garg,&nbsp;Sheetal Kumari,&nbsp;Minh-Ky Nguyen,&nbsp;Basil Osayin Daudu,&nbsp;Sanskriti Thapa,&nbsp;Juhi Gupta,&nbsp;Manish Kumar,&nbsp;Elif Esra Altuner,&nbsp;Akhilesh Kumar Yadav,&nbsp;Esther Besty Anaiye,&nbsp;Adel Zrelli,&nbsp;Kassian T. T. Amesho","doi":"10.1007/s10532-026-10304-y","DOIUrl":"10.1007/s10532-026-10304-y","url":null,"abstract":"<div><p>Green chemistry is defined as a set of principles that reduce or prevent the use or generation of hazardous substances during the design, production, and utilization of chemical products. The vision of such a paradigm shift in the chemical sciences is that the concept of being green is directly introduced to the molecular design process and is centered on atom economy and the prevention of waste. This review examines the principles of green chemistry in relation to agro-industrial waste valorization, with specific reference to the ecological and economic conditions of India, where approximately 350 million metric tons of annual agro-residues have become a source of serious environmental management issues, such as greenhouse gas emissions through open burning, leachate waste generation through landfills, and effects on the health of the population through poor disposal practices. The analysis summarizes the latest developments in nanotechnology-based catalytic systems, new solvent platforms (ionic liquids, deep eutectic solvents, and supercritical fluids), and integrated biorefineries, and critically reviews the scalability limitations and commercial feasibility. It also discusses more recent developments, such as systems based on nanotechnology, catalyst transformations (homogeneous, heterogeneous, and biocatalysts), and the creation of alternative solvents, such as ionic liquids, deep eutectic solvents, and supercritical fluids. The virtues of agri-industrial residues and biomass-based feeds are given particular attention in terms of their role in models of the circular economy and the generation of value-added chemicals, fuels, and materials. By illustrating how green chemistry can minimize the environmental footprint of traditional processes and create safer and more economically viable alternatives, this review makes it clear why green chemistry has become a revolution in the field of industrial practice. Lastly, the paper addresses contemporary issues of scalability, economic competitiveness, and regulatory integration and outlines opportunities that will make green chemistry the foundation of sustainable, resource-efficient, and environmentally responsible chemical companies.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced spent oil bioremediation in contaminated soils using biostimulants: kinetics, half-life, and efficacy assessment","authors":"C. Nna Orji","doi":"10.1007/s10532-026-10306-w","DOIUrl":"10.1007/s10532-026-10306-w","url":null,"abstract":"<div><p>The persistence of petroleum hydrocarbons in contaminated soils remains a significant environmental concern, necessitating effective and sustainable remediation strategies. This study evaluated the potential of selected ecofriendly organic biostimulants: egusi peel (Ymp), sawdust (Sd), and cassava peel (Csp), applied individually and in combination, to enhance the biodegradation of spent oil in contaminated soil over a 180-day period under controlled microcosm conditions. Biodegradation efficiency was assessed by monitoring residual oil concentrations at regular intervals. Kinetic models (zero-, first-, second-, and third-order) were applied to describe degradation patterns and estimate rate constants. All amended treatments showed improved hydrocarbon reduction compared to the untreated control. The combination of Ymp + Sd exhibited the highest performance, with an average degradation rate of 0.269%/day and 48.42% removal, while Ymp alone also showed notable efficiency. Kinetic analysis indicated variable degradation behavior across treatments while statistical differences (p &lt; 0.001), among treatments were observed. Complementary correlation and principal component analyses further elucidated relationships between biostimulants properties and performance. The findings demonstrated that ecofriendly agro-waste biostimulants enhanced hydrocarbon biodegradation and highlighted promising biostimulant formulations; however, further studies incorporating microbial characterization and field validation are required.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and screening of silicate-solubilizing bacteria for enhanced desilicification of silica-containing agro-residues","authors":"Oinam Bidyarani Devi,&nbsp;Federico Cerrone,&nbsp;Mohit Kumar,&nbsp;Deepali Rani,&nbsp;Santosh Kumar Mishra","doi":"10.1007/s10532-026-10309-7","DOIUrl":"10.1007/s10532-026-10309-7","url":null,"abstract":"<div><p>Silica solubilizing bacteria (SSB) play a vital role in the silicon (Si) cycle by producing the enzyme silicase, which converts insoluble silicates into bioavailable silicic acid, replenishing bio-available Si in soil. Si, a beneficial element for silica-rich plants, is depleted annually due to plants uptake, intensive agriculture, and slow silicate bio-weathering. This study aims to isolate novel SSB with an enhanced silicate desilicification potential from rice field soil. Primary screening for silica solubilization ability was performed on silicate glucose agar (SGA). The selected bacterial strain was evaluated for silicase production under submerged fermentation (SmF) condition using a modified Horikoshi medium. Then, molecular identification was performed by 16S rRNA sequencing analysis and confirmed for silicic acid formation by FTIR-ATR analysis. The investigation resulted with a bacterial isolate forming a clear zone with a solubilization index (SI) of 3.3. Subsequently, 16S rRNA gene sequencing analysis confirmed the isolate as <i>Etopseudomonas mendocina</i>. Further a comparative silicase activity of the bacterial isolate showed its maximum activity of 1.67 ± 0.06U/mL on in rice husk against other agro residues viz, rice straw, wheat husk and wheat straw. FTIR-ATR analyses confirmed the presence of silicic acid in the crude extracts of the agro residues after culture with the bacterial isolate, with characteristic peaks at 3400–3200, 1600–1650, and 1100–1300 cm⁻¹. The research findings reveal that the bacterial isolate effectively biodegrade silica-rich agricultural residues and may have potential applications in agro-residue management. These degradative products could be used as Si-based biofertilizers for sustainable agricultural practices.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147938806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naproxen as an emerging environmental contaminant: Microbial degradation mechanism and optimization strategies","authors":"Irdeana Marsha Baharuddin,&nbsp;Siti Khadijah Awang,&nbsp;Muhammad Iqbal Mashudi,&nbsp;Wan Iryani Wan Ismail,&nbsp;Fazilah Ariffin","doi":"10.1007/s10532-026-10303-z","DOIUrl":"10.1007/s10532-026-10303-z","url":null,"abstract":"<div><p>Naproxen, a non-steroidal anti-inflammatory drug (NSAID), has emerged as a contaminant of concern due to its environmental persistence, incomplete removal by conventional wastewater treatment processes, and associated ecological risks. It has been detected at concentrations ranging from nanograms to micrograms per liter in surface waters, wastewater effluents, and, in some cases, drinking water, particularly in regions with limited treatment infrastructure. Several bacterial genera, such as <i>Pseudomonas</i> and <i>Bacillus</i>, have been reported to transform naproxen through hydroxylation and ring-modification reactions catalyzed by specific enzymes. Microbial consortia, when provided with optimal pH, temperature, oxygen, and nutrient conditions, demonstrate greater degradation efficiency compared to single strains. Despite extensive research on pharmaceutical pollution, a comprehensive understanding of naproxen biodegradation and the environmental factors influencing its removal remains limited. This review synthesizes current knowledge on the occurrence of naproxen in aquatic environments, microbial degradation pathways, and the influence of the combined effects of microbial characteristics and abiotic parameters, including pH, temperature, oxygen availability, and nutrient supply, on degradation efficiency. Several key knowledge gaps remain, including an incomplete understanding of metabolic pathways utilized by diverse microbial communities, the impact of emerging contaminants on naproxen degradation, and the long-term ecological implications of residual naproxen in treated effluents. In addition, challenges related to scaling laboratory findings to real-world applications and integrating biodegradation with other treatment technologies require further investigation. By integrating dispersed findings into a unified conceptual framework, this review identifies critical knowledge gaps and proposes future research directions to advance biologically based strategies for pharmaceutical wastewater treatment.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147873001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of ammonia using native bacterial strains and developed synthetic consortia from aquaculture wastewater in Tuticorin, Southeast coast of India","authors":"Kannaiyan Kumaresan Shrivasanthan,&nbsp;Durairaj Manimekalai,&nbsp;Pandurangan Padmavathy,&nbsp;Pandi Ganesan,&nbsp;Muthu Ponmani,&nbsp;Sridhar Sakthi","doi":"10.1007/s10532-026-10310-0","DOIUrl":"10.1007/s10532-026-10310-0","url":null,"abstract":"<div><p>Excessive ammonia accumulation in aquaculture effluents poses significant ecological and production challenges, necessitating sustainable remediation approaches. The present study reports the isolation and characterization of indigenous ammonia-utilizing bacteria from shrimp pond wastewater in Tuticorin, Southeast coast of India, and a comparative assessment of their utilization efficiencies as individual strains and synthetic consortia. Eleven isolates belonging to <i>Bacillus, Pseudomonas, Burkholderia, Nitrosomonas, Rhizobacterium</i>, and <i>Agrobacterium</i> spp. were identified through phenotypic characterization and 16S rRNA sequencing. Individual strains demonstrated 30–70% ammonia removal within 10 days, with <i>Nitrosomonas</i> sp. exhibiting the highest efficiency (70%). Based on compatibility assays, five three-species consortia (C1–C5) were formulated. Among these, consortia C3 (<i>Bacillus amyloliquefaciens, Burkholderia paludis, Nitrosomonas</i> sp.) and C4 (<i>Burkholderia cepacia, Nitrosomonas</i> sp.<i>, Burkholderia seminalis</i>) achieved superior performance, attaining approximately 85% ammonia utilization by day 10 under optimal conditions. The relatively balanced distribution within the consortia suggested potential cooperative or niche-partitioning effects. These results underscore the importance of species compatibility when designing microbial consortia and potential of synergistic indigenous microbial consortia as effective bioaugmentation agents for aquaculture wastewater treatment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147872957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the phytoremediation capacity of Verbesina encelioides through lead-induced morphological and biochemical shifts","authors":"Kiran Choudhary,&nbsp;Afroz Alam,&nbsp;Mohammad Mehdizadeh,&nbsp;Hussein A. H. Said-Al Ahl,&nbsp;Kirill Tkachenko,&nbsp;Wafaa M. Hikal","doi":"10.1007/s10532-026-10307-9","DOIUrl":"10.1007/s10532-026-10307-9","url":null,"abstract":"<div><p>Soil contamination with heavy metals, particularly lead (Pb), has become a critical environmental issue due to rapid urbanization and industrial activities. This study was undertaken to evaluate the tolerance, physiological responses, and Pb accumulation behaviour of <i>Verbesina encelioides</i> under increasing Pb stress, in order to assess its potential for phytoremediation, particularly phytostabilization, in contaminated soils. To achieve this, a controlled pot experiment was conducted using graded Pb concentrations (0–500 mg kg⁻¹ soil), and plant responses were evaluated at different growth stages. Lead stress adversely affected the growth and physiological performance of <i>V. encelioides</i>. Lead stress impaired the growth and physiological functions of <i>V. encelioides</i>. Exposure to 500 mg kg⁻¹ Pb reduced shoot length and dry weight by approximately 45 and 60%, respectively and root length and weight 17–32 and 42% respectively, compared to the control (<i>p</i> ≤ 0.05), while leaf area declined by over 50%. Lead accumulation was predominantly confined to roots, with concentrations up to eightfold higher than in aerial parts, indicating restricted translocation. Photosynthetic pigments were severely affected, with chlorophyll <i>a</i> and total chlorophyll decreasing by more than 50% at the highest Pb level. Biochemically, the plant activated defence mechanisms, as shown by an increase in proline content and the activities of peroxidase (3–11 fold) and catalase (4–10 fold) by several-fold at 500 mg kg⁻¹ compared with the control (<i>p</i> ≤ 0.05). These morphological inhibitions and biochemical adjustments suggest that resources are preferentially allocated to stress mitigation processes, thereby constraining growth performance. Lead accumulation was predominantly root-localized, with shoot bioaccumulation factor (BAF) values remaining below unity (0.01–0.27) across all treatments and sampling intervals, indicating lead immobilization and restricted translocation to aerial tissues. Overall, the findings indicate that <i>V. encelioides</i> possesses adaptive traits that may support its use in phytostabilization rather than phytoextraction under controlled experimental conditions.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation dynamics: an insight into microbial interactions with explosive compounds","authors":"Vandana Singh,&nbsp;Mohammed E. Abo-El Fetoh,&nbsp;Islam E. Abo-El Fetoh","doi":"10.1007/s10532-026-10299-6","DOIUrl":"10.1007/s10532-026-10299-6","url":null,"abstract":"<div><p>Degradation dynamics is an essential aspect in the field of environmental science and is crucial in understanding the interaction between microbes and explosive compounds. Explosive compounds and their residues, such as nitramines, nitro-substituted aromatics, picric acid, TETRYL, and HEXYL), and aliphatic, RDX, etc.are highly persistent in the environment. These compounds are toxic to many life forms at high concentrations, specific microbial species have evolved resistance and degradation capabilities, though their growth can still be inhibited beyond certain thresholds, The results of microbial biodegradation can range from complete mineralization to only the biotransformation into less toxic or more resistant metabolites. Research using pure cultures of bacteria and fungi has provided insight into the degradation pathways of certain nitro-organic compounds, and some key enzymes (laccases and lignin peroxidases) have been identified and studied. This review mainly aims to provide an overview of the current state of research on the degradation dynamics of explosive compounds Recent advancements have pivoted toward 'Bio-omics' and synthetic biology tools, such as CRISPR/Cas systems, to engineer high-activity microbial strains.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial desulfurization of low-grade Balochistan coal using Bacillus licheniformis and Bacillus pumilus","authors":"Asif Naeem,&nbsp;Maqsood Ahmed,&nbsp; Asadullah,&nbsp;Jamal Khan,&nbsp;Zahid Naeem Qaisrani","doi":"10.1007/s10532-026-10305-x","DOIUrl":"10.1007/s10532-026-10305-x","url":null,"abstract":"<div><p>Coal is an abundant source of energy; however, the use of Pakistani coal is limited due to its high sulfur content, which causes environmental and health issues. Hence, pre-combustion desulfurization of coal is imperative for quality enhancement. The present study investigates the microbial desulfurization of coal from the Balochistan mine by using local bacterial strains, <i>Bacillus pumilus</i> and <i>Bacillus licheniformis</i>, isolated from the same coal mine, for their sustainability, adaptability, and local availability. Batch incubation experiments with a coal particle size of 75 and 150 µm and incubation periods of 7 and 14 days were conducted to evaluate the effect of particle size and incubation period on the sulfur removal efficiency. Proximate analysis was conducted in accordance with ASTM D3173, ASTM D3174, and ASTM D3175 to determine the moisture content (MC), ash content (AC), and volatile matter (VM), while ultimate analysis was performed using a CHNS analyzer. Additionally, XRD was used to determine changes in composition and structure before and after treatment. The results showed that microbial treatment increased calorific value, specifically at smaller particle sizes and longer incubation periods. The maximum sulfur removal achieved by <i>B. licheniformis</i> was 32.73% and 41.06%, whereas <i>B. pumilus</i> exhibited a removal of 20.75% and 33.48% at a particle size of 75 µm after 7 and 14 days, respectively. These findings demonstrate that bio-desulfurization using indigenous microorganisms is an effective and environmentally sustainable approach for upgrading low-grade Pakistani coal for cleaner energy production.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing soil microbiome alterations in agricultural land exposed to pesticides through amplicon-based sequencing","authors":"Srishti Sinha Ray,&nbsp;Nishu Goyal,&nbsp;Madhumita Bhattacharyya,&nbsp;Smriti Arora,&nbsp;Kanchan Deoli Bahukhandi,&nbsp;Durga Madhab Mahapatra","doi":"10.1007/s10532-026-10302-0","DOIUrl":"10.1007/s10532-026-10302-0","url":null,"abstract":"<div><p>The degradation of soil health due to intensive pesticide application has emerged as a critical global challenge, undermining ecological sustainability and agricultural productivity. In regions of high agronomic activity such as Dehradun, India, unsustainable practices including monocropping and excessive agrochemical inputs have been implicated in the decline of soil fertility and microbial diversity. This study employs 16S rRNA gene (V3–V4) amplicon-based sequencing to characterize shifts in bacterial community structure for agricultural farming and non-farming soils. Complementary physicochemical analyses revealed significant associations between soil health parameters and microbial community dynamics. Taxonomic profiling revealed distinct microbial signatures in pesticide-contaminated soils, with a notable enrichment of the phyla Proteobacteria, Acidobacteria, Firmicutes, and Actinobacteria<i>.</i> Dominant genera such as <i>Bacillus</i> sp.,<i> Chungangia</i> sp., and <i>Streptomyces</i> sp. were identified, indicating their potential functional roles in biogeochemical cycling and adaptive resilience under chemical stress. Functional prediction using PICRUSt2 highlighted key microbial pathways associated with amino acid synthesis, fatty acid synthesis, degradation of aromatic compound, and other essential biochemical processes. These findings highlight the ecological significance of microbial communities in maintaining soil functionality and offer insights into the development of sustainable land management strategies in pesticide-impacted agroecosystems.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and MALDI–TOF MS‑based identification of new bacterial isolates from greenhouse and mulch film fields in Izmir (Turkiye) for LDPE biodegradation","authors":"Arda Yavasoglu,&nbsp;Huseyin Cinarli,&nbsp;Tuna Aras,&nbsp;Ziydal Zor Alpugan","doi":"10.1007/s10532-026-10301-1","DOIUrl":"10.1007/s10532-026-10301-1","url":null,"abstract":"<div><p>Low density polyethylene (LDPE) plastic films, which are widely used in greenhouses and mulch areas in the agricultural sector, can remain in the environment for a long time due to their resistance to decomposition structures and create large amounts of plastic pollution. In this study, it was aimed to isolate and identify bacteria which are capable of polyethylene (PE) degradation from greenhouse and mulch film soils containing plastic wastes in Izmir, one of the cities where greenhouse farming is most common in Türkiye, and to determine the degradation potentials of these bacteria. Bacteria were screened in soil samples taken from different greenhouses, mulched film areas and garden areas containing plastic waste, which have been covered with PE film for 10–30 years. Eight bacterial isolates were identified for the first time from these areas using the matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system. Plastic degradation properties of bacterial isolates were demonstrated after 30-days degradation study by reduction in PE film weight, FTIR–ATR analysis to determine structural changes on these films and SEM imaging to visualize changes in surface morphology. It was determined that the reduction rates in PE film weights of individual bacterial isolates after 30 days varied between 25.39 and 7.08%, and the highest weight loss was recorded in <i>Acinetobacter pittii</i> HAT01. Also, the bacterium was observed to have biofilm formation capacity. FTIR–ATR analysis and SEM imaging findings of PE films supported the weight loss findings. This study provides new candidate strains adapted to long-term LDPE-contaminated agricultural soils and contributes to the development of sustainable biodegradation strategies.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"37 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}