Biodegradation最新文献

{"title":"Bioaugmentation: an approach to biological treatment of pollutants","authors":"Dixita Chettri,&nbsp;Ashwani Kumar Verma,&nbsp;Anil Kumar Verma","doi":"10.1007/s10532-023-10050-5","DOIUrl":"10.1007/s10532-023-10050-5","url":null,"abstract":"<div><p>Industrial development and the associated generation of waste requires attention for their management, treatment, and reduction without further degrading the quality of life. Microbes and plant-based bioremediation approaches are some of the sustainable strategies for the biodegradation of harmful pollutants instead of chemical-based treatment. Bioaugmentation is one such approach where microbial strains with the ability to degrade the targeted pollutant are introduced in a polluted environment. Harnessing of microbes from various locations, especially from the site of contamination (indigenous microbes), followed by optimization of the strains, inoculum size, media, and genetic engineering of the microbes along with a combination of strategies such as bio stimulation, phytoremediation is being applied to increase the efficiency of bioaugmentation. Further, bioaugmentation is influenced by various factors such as temperature, the composition of the pollutant, and microbial inoculum which needs to be considered for maximum efficiency of the treatment process. It has numerous advantages such as low cost, sustainability, and easy handling of the contaminants however, the major limitation of bioaugmentation is to increase the survival rate of the microbes involved in remediation for a longer duration in such a highly toxic environment. The review discusses these various aspects of bioaugmentation in brief for its large-scale implementation to address the global issue of pollution and environment management.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10190940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of nomenclature and methods of analysis of polyethylene terephthalic acid hydrolyzing enzymes activity","authors":"Ruth Amanna,&nbsp;Sudip K. Rakshit","doi":"10.1007/s10532-023-10048-z","DOIUrl":"10.1007/s10532-023-10048-z","url":null,"abstract":"<div><p>Enzymatic degradation of polyethylene terephthalic acid (PET) has been gaining increasing importance. This has resulted in a significant increase in the search for newer enzymes and the development of more efficient enzyme-based systems. Due to the lack of a standard screening process, screening new enzymes has relied on other assays to determine the presence of esterase activity. This, in turn, has led to various nomenclatures and methods used to describe them and measure their activity. Since all PET-hydrolyzing enzymes are α/β hydrolases, they catalyze a serine nucleophilic attack and cleave an ester bond. They are lipases, esterases, cutinases and hydrolases. This has been used interchangeably, leading to difficulties while comparing results and evaluating progress. This review discusses the varied enzyme nomenclature being adapted, the different assays and analysis methods reported, and the strategies used to increase PET-hydrolyzing enzyme efficiency. A section on the various ways to quantify PET hydrolysis is also covered.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 4","pages":"341 - 360"},"PeriodicalIF":3.1,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Landfill leachate treatment using fungi and fungal enzymes: a review","authors":"Anusree Nalladiyil,&nbsp;P. Sughosh,&nbsp;G. L. Sivakumar Babu,&nbsp;Sreenivasan Ramaswami","doi":"10.1007/s10532-023-10052-3","DOIUrl":"10.1007/s10532-023-10052-3","url":null,"abstract":"<div><p>Landfill leachate raises a huge risk to human health and the environment as it contains a high concentration of organic and inorganic contaminants, heavy metals, ammonia, and refractory substances. Among leachate treatment techniques, the biological methods are more environmentally benign and less expensive than the physical–chemical treatment methods. Over the last few years, fungal-based treatment processes have become popular due to their ability to produce powerful oxidative enzymes like peroxidases and laccases. Fungi have shown better removal efficiency in terms of color, ammonia, and COD. However, their use in the treatment of leachate is relatively recent and still needs to be investigated. This review article assesses the potential of fungi and fungal-derived enzymes in treating landfill leachate. The review also compares different enzymes involved in the fungal catabolism of organic pollutants and the enzyme degradation mechanisms. The effect of parameters like pH, temperature, contact time, dosage variation, heavy metals and ammonia are discussed. The paper also explores the reactor configuration used in the fungal treatment and the techniques used to improve leachate treatment efficacy, like pretreatment and fungi immobilisation. Finally, the review summarises the limitations and the future direction of work required to adapt the fungal application for leachate treatment on a large scale.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10181128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myco-remediation of plastic pollution: current knowledge and future prospects","authors":"Somanjana Khatua,&nbsp;Jesus Simal-Gandara,&nbsp;Krishnendu Acharya","doi":"10.1007/s10532-023-10053-2","DOIUrl":"10.1007/s10532-023-10053-2","url":null,"abstract":"<div><p>To date, enumerable fungi have been reported to participate in the biodegradation of several notorious plastic materials following their isolation from soil of plastic-dumping sites, marine water, waste of mulch films, landfills, plant parts and gut of wax moth. The general mechanism begins with formation of hydrophobin and biofilm proceding to secretion of specific plastic degarding enzymes (peroxidase, hydrolase, protease and urease), penetration of three dimensional substrates and mineralization of plastic polymers into harmless products. As a result, several synthetic polymers including polyethylene, polystyrene, polypropylene, polyvinyl chloride, polyurethane and/or bio-degradable plastics have been validated to deteriorate within months through the action of a wide variety of fungal strains predominantly Ascomycota (<i>Alternaria</i>, <i>Aspergillus</i>, <i>Cladosporium</i>, <i>Fusarium</i>, <i>Penicillium</i> spp.). Understanding the potential and mode of operation of these organisms is thus of prime importance inspiring us to furnish an up to date view on all the presently known fungal strains claimed to mitigate the plastic waste problem. Future research henceforth needs to be directed towards metagenomic approach to distinguish polymer degrading microbial diversity followed by bio-augmentation to build fascinating future of waste disposal.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10950981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10144584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of commercial textile reactive dye mixtures by industrial effluent adapted bacterial consortium VITPBC6: a potential technique for treating textile effluents","authors":"Purbasha Saha,&nbsp;Kokati Venkata Bhaskara Rao","doi":"10.1007/s10532-023-10047-0","DOIUrl":"10.1007/s10532-023-10047-0","url":null,"abstract":"<div><p>Textile industries release major fraction of dyestuffs in effluents leading to a major environmental concern. These effluents often contain more than one dyestuff, which complicates dye degradation. In this study ten reactive dyes (Reactive Yellow 145, Reactive Yellow 160, Reactive Orange 16, Reactive Orange 107, Reactive Red 195, Reactive Blue 21, Reactive Blue 198, Reactive Blue 221, Reactive Blue 250, and Reactive Black 5) that are used in textile industries were subjected to biodegradation by a bacterial consortium VITPBC6, formulated in our previous study. Consortium VITPBC6 caused single dye degradation of all the mentioned dyes except for Reactive Yellow 160. Further, VITPBC6 efficiently degraded a five-dye mixture (Reactive Red 195, Reactive Orange 16, Reactive Black 5, Reactive Blue 221, and Reactive Blue 250). Kinetic studies revealed that the five-dye mixture was decolorized by VITPBC6 following zero order reaction kinetic; V_max and K_m values of the enzyme catalyzed five-dye decolorization were 128.88 mg L⁻¹ day⁻¹ and 1003.226 mg L⁻¹ respectively. VITPBC6 degraded the dye mixture into delta-3,4,5,6-Tetrachlorocyclohexene, sulfuric acid, 1,2-dichloroethane, and hydroxyphenoxyethylaminohydroxypropanol. Phytotoxicity, cytogenotoxicity, microtoxicity, and biotoxicity assays conducted with the biodegraded metabolites revealed that VITPBC6 lowered the toxicity of five-dye mixture significantly after biodegradation.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10503043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and characterization of extracellular PHB depolymerase enzyme from Aeromonas caviae Kuk1-(34) and their biodegradation studies with polymer films","authors":"Mohd. Amir,&nbsp;Naushin Bano,&nbsp;Anamika Gupta,&nbsp;Mohd. Rehan Zaheer,&nbsp; Roohi","doi":"10.1007/s10532-023-10051-4","DOIUrl":"10.1007/s10532-023-10051-4","url":null,"abstract":"<div><p>PHB depolymerase enzymes are able to breakdown the PHB polymers and thereby get significant economic value in the bioplastics industry and for bioremediation as well. This study shows the purification of novel extracellular PHB depolymerase enzyme from <i>Aeromonas caviae</i> Kuk1-(34) using dialysis followed by gel filtration and HPLC. The purification fold and yield after HPLC were 45.92 and 27.04%, respectively. HPLC data showed a single peak with a retention time of 1.937 min. GC-MS analysis reveals the presence of three compounds, of which 1-Dodecanol was found to be most significant with 54.48% area and 8.623-min retention time (RT). The molecular weight of the purified enzyme was obtained as 35 kDa with K_m and apparent V_max values of 0.769 mg/mL and 1.89 U/mL, respectively. The enzyme was moderately active at an optimum temperature of 35 °C and at pH 8.0. The stability was detected at pH 7.0–9.0 and 35–45 °C. Complete activity loss was observed with EDTA, SDS, Tween-20 at 5 mM and with 0.1% Triton X 100. A biodegradation study of commercially available biodegradable polymer films was carried out in a liquid medium and in soil separately with pure microbial culture and with purified enzyme for 7, 14, 28, and 49 consecutive days. In a liquid medium, with a pure strain of <i>Aeromonas caviae</i> Kuk1-(34), the maximum degradation (89%) was achieved on the PHB film, while no changes were observed with other polymer films. With purified enzyme in the soil, 71% degradation of the PHB film was noticed, and it was only 18% in the liquid medium. All such weight analysis were confirmed by SEM images where several holes, pits, grooves, crest, and surface roughness are clearly observed. Our results demonstrated the potential utility of <i>Aeromonas caviae</i> Kuk1-(34) as a source of extracellular PHB depolymerase capable of degrading PHB under a wide range of natural/ lab conditions.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10440243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biooxidation of hydrogen sulfide to sulfur by moderate thermophilic acidophilic bacteria","authors":"R. Romero,&nbsp;P. Viedma,&nbsp;D. Cotoras","doi":"10.1007/s10532-023-10049-y","DOIUrl":"10.1007/s10532-023-10049-y","url":null,"abstract":"<div><p>The copper industry utilizes significant amounts of sulfuric acid in its processes, generating sulfate as waste. While sulfate-reducing bacteria can remove sulfate, it produces hydrogen sulfide (H₂S) as a byproduct. This study examined the capability of a consortium consisting of <i>Sulfobacillus thermosulfidooxidans</i> and <i>Sulfobacillus acidophilus</i> to partially oxidize H₂S to S° at a temperature of 45 °C. A fixed-bed bioreactor, with glass rings as support material and sodium thiosulfate as a model electron donor, was inoculated with the consortium. Formation of biofilms was crucial to maintain the bioreactor’s steady state, despite high flow rates. Afterward, the electron donor was changed to H₂S. When the bioreactor was operated continuously and with high aeration, H₂S was fully oxidized to SO₄²⁻. However, under conditions of low aeration and at a concentration of 0.26 g/L of H₂S, the consortium was able to oxidize H₂S to S° with a 13% yield. S° was discovered attached to the glass rings and jarosite. The results indicate that the consortium could oxidize H₂S to S° with a 13% yield under low aeration and at a concentration of 0.26 g/L of H₂S. The findings highlight the capability of a <i>Sulfobacillus</i> consortium to convert H₂S into S°, providing a potential solution for addressing environmental and safety issues associated with sulfate waste generated by the mining industry.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10440242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaugmentation has temporary effect on anaerobic pesticide biodegradation in simulated groundwater systems","authors":"Andrea Aldas-Vargas,&nbsp;Jannigje G. Kers,&nbsp;Hauke Smidt,&nbsp;Huub H. M. Rijnaarts,&nbsp;Nora B. Sutton","doi":"10.1007/s10532-023-10039-0","DOIUrl":"10.1007/s10532-023-10039-0","url":null,"abstract":"<div><p>Groundwater is the most important source for drinking water in The Netherlands. Groundwater quality is threatened by the presence of pesticides, and biodegradation is a natural process that can contribute to pesticide removal. Groundwater conditions are oligotrophic and thus biodegradation can be limited by the presence and development of microbial communities capable of biodegrading pesticides. For that reason, bioremediation technologies such as bioaugmentation (BA) can help to enhance pesticide biodegradation. We studied the effect of BA using enriched mixed inocula in two column bioreactors that simulate groundwater systems at naturally occurring redox conditions (iron and sulfate-reducing conditions). Columns were operated for around 800 days, and two BA inoculations (BA1 and BA2) were conducted in each column. Inocula were enriched from different wastewater treatment plants (WWTPs) under different redox-conditions. We observed a temporary effect of BA1, reaching 100% removal efficiency of the pesticide 2,4-D after 100 days in both columns. In the iron-reducing column, 2,4-D removal was in general higher than under sulfate-reducing conditions demonstrating the influence of redox conditions on overall biodegradation. We observed a temporary shift in microbial communities after BA1 that is relatable to the increase in 2,4-D removal efficiency. After BA2 under sulfate-reducing conditions, 2,4-D removal efficiency decreased, but no change in the column microbial communities was observed. The present study demonstrates that BA with a mixed inoculum can be a valuable technique for improving biodegradation in anoxic groundwater systems at different redox-conditions.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10951022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9775119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autochthonous psychrophilic hydrocarbonoclastic bacteria and its ecological function in contaminated cold environments","authors":"Pranjal Bharali,&nbsp;Bhagyudoy Gogoi,&nbsp;Viphrezolie Sorhie,&nbsp;Shiva Aley Acharjee,&nbsp;Bendangtula Walling,&nbsp; Alemtoshi,&nbsp;Vinita Vishwakarma,&nbsp;Maulin Pramod Shah","doi":"10.1007/s10532-023-10042-5","DOIUrl":"10.1007/s10532-023-10042-5","url":null,"abstract":"<div><p>Petroleum hydrocarbon (PH) pollution has mostly been caused by oil exploration, extraction, and transportation activities in colder regions, particularly in the Arctic and Antarctic regions, where it serves as a primary source of energy. Due to the resilience feature of nature, such polluted environments become the realized ecological niches for a wide community of psychrophilic hydrocarbonoclastic bacteria (PHcB). In contrast, to other psychrophilic species, PHcB is extremely cold-adapted and has unique characteristics that allow them to thrive in greater parts of the cold environment burdened with PHs. The stated group of bacteria in its ecological niche aids in the breakdown of litter, turnover of nutrients, cycling of carbon and nutrients, and bioremediation. Although such bacteria are the pioneers of harsh colder environments, their growth and distribution remain under the influence of various biotic and abiotic factors of the environment. The review discusses the prevalence of PHcB community in colder habitats, the metabolic processes involved in the biodegradation of PH, and the influence of biotic and abiotic stress factors. The existing understanding of the PH metabolism by PHcB offers confirmation of excellent enzymatic proficiency with high cold stability. The discovery of more flexible PH degrading strategies used by PHcB in colder environments could have a significant beneficial outcome on existing bioremediation technologies. Still, PHcB is least explored for other industrial and biotechnological applications as compared to non-PHcB psychrophiles. The present review highlights the pros and cons of the existing bioremediation technologies as well as the potential of different bioaugmentation processes for the effective removal of PH from the contaminated cold environment. Such research will not only serve to investigate the effects of pollution on the basic functional relationships that form the cold ecosystem but also to assess the efficacy of various remediation solutions for diverse settings and climatic conditions.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 1","pages":"1 - 46"},"PeriodicalIF":3.1,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9826984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anammox with alternative electron acceptors: perspectives for nitrogen removal from wastewaters","authors":"Sergio J. Ponce-Jahen,&nbsp;Bibiana Cercado,&nbsp;Edson Baltazar Estrada-Arriaga,&nbsp;J. Rene Rangel-Mendez,&nbsp;Francisco J. Cervantes","doi":"10.1007/s10532-023-10044-3","DOIUrl":"10.1007/s10532-023-10044-3","url":null,"abstract":"<div><p>In the context of the anaerobic ammonium oxidation process (anammox), great scientific advances have been made over the past two decades, making anammox a consolidated technology widely used worldwide for nitrogen removal from wastewaters. This review provides a detailed and comprehensive description of the anammox process, the microorganisms involved and their metabolism. In addition, recent research on the application of the anammox process with alternative electron acceptors is described, highlighting the biochemical reactions involved, its advantages and potential applications for specific wastewaters. An updated description is also given of studies reporting the ability of microorganisms to couple the anammox process to extracellular electron transfer to insoluble electron acceptors; particularly iron, carbon-based materials and electrodes in bioelectrochemical systems (BES). The latter, also referred to as anodic anammox, is a promising strategy to combine the ammonium removal from wastewater with bioelectricity production, which is discussed here in terms of its efficiency, economic feasibility, and energetic aspects. Therefore, the information provided in this review is relevant for future applications.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 1","pages":"47 - 70"},"PeriodicalIF":3.1,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10774155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10129524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}