Optimization of polyhydroxyalkanoate (PHA) production from biohythane pilot plant effluent by Cupriavidus necator TISTR 1335

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2025-01-20 DOI:10.1007/s10532-025-10110-y

Yu-Ting Song, Napapat Sitthikitpanya, Nikannapas Usmanbaha, Alissara Reungsang, Chen-Yeon Chu

{"title":"Optimization of polyhydroxyalkanoate (PHA) production from biohythane pilot plant effluent by Cupriavidus necator TISTR 1335","authors":"Yu-Ting Song, Napapat Sitthikitpanya, Nikannapas Usmanbaha, Alissara Reungsang, Chen-Yeon Chu","doi":"10.1007/s10532-025-10110-y","DOIUrl":null,"url":null,"abstract":"<div><p>Bioplastics, particularly polyhydroxyalkanoates (PHAs), are emerging as promising alternatives to traditional materials due to their biodegradability. This study focuses on the production of PHAs as bioplastics using effluent from hydrogen production in a two-stage Biohythane Pilot Plant, which provides a low-cost substrate. The aim is to optimize production conditions, with <i>Cupriavidus necator</i> TISTR 1335 being used as the PHA producer. Utilizing Response Surface Methodology-Central Composite Design, we explored optimal conditions, revealing peak PHA production at a substrate concentration of 33.51 g COD/L and a pH of 6.87. The predicted optimal PHA concentration was at 3.05 g/L within the established model, closely matching the experimentally validated value of 3.02 g/L, with the overall usage rate of reducing sugars approximately 50–60%. This study underscores the importance of optimizing PHA production conditions and paving the way toward large-scale PHA production.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biodegradation","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10532-025-10110-y","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Bioplastics, particularly polyhydroxyalkanoates (PHAs), are emerging as promising alternatives to traditional materials due to their biodegradability. This study focuses on the production of PHAs as bioplastics using effluent from hydrogen production in a two-stage Biohythane Pilot Plant, which provides a low-cost substrate. The aim is to optimize production conditions, with Cupriavidus necator TISTR 1335 being used as the PHA producer. Utilizing Response Surface Methodology-Central Composite Design, we explored optimal conditions, revealing peak PHA production at a substrate concentration of 33.51 g COD/L and a pH of 6.87. The predicted optimal PHA concentration was at 3.05 g/L within the established model, closely matching the experimentally validated value of 3.02 g/L, with the overall usage rate of reducing sugars approximately 50–60%. This study underscores the importance of optimizing PHA production conditions and paving the way toward large-scale PHA production.

Abstract Image

查看原文本刊更多论文

Cupriavidus necator tist1335生物乙烷中试废水生产聚羟基烷酸酯（PHA）的优化

生物塑料，特别是聚羟基烷酸酯（pha），由于其可生物降解性，正成为传统材料的有希望的替代品。本研究的重点是在一个两阶段的生物乙烷中试工厂中利用氢气生产的废水生产pha作为生物塑料，该工厂提供了一种低成本的基质。目的是优化生产条件，以Cupriavidus necator TISTR 1335作为PHA生产者。利用响应面法-中心复合设计，我们探索了最佳条件，发现底物浓度为33.51 g COD/L， pH为6.87时PHA产量最高。在建立的模型内，预测的最佳PHA浓度为3.05 g/L，与实验验证值3.02 g/L非常接近，总体还原糖利用率约为50-60%。这项研究强调了优化PHA生产条件和为PHA大规模生产铺平道路的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.