Biodegradation最新文献

{"title":"Removal mechanisms of pentachlorophenol in a horizontal-flow anaerobic immobilized biomass reactor (HAIB) inoculated with an indigenous estuarine sediment microbiota: adsorption and biodegradation processes","authors":"Gunther Brucha,&nbsp;Alessandra Giordani,&nbsp;Bárbara Franco Vieira,&nbsp;Marcia Helena Rissato Zamariolli Damianovic,&nbsp;Flavia Talarico Saia,&nbsp;Leonardo Henrique Soares Damasceno,&nbsp;Johannes Gerson Janzen,&nbsp;Eugenio Foresti,&nbsp;Rosana Filomena Vazoller","doi":"10.1007/s10532-024-10096-z","DOIUrl":"10.1007/s10532-024-10096-z","url":null,"abstract":"<div><p>Pentachlorophenol (PCP) is a highly toxic and carcinogenic compound with significant environmental impact, necessitating effective treatment technologies. This study evaluates PCP removal mechanisms, including adsorption and biodegradation, during the startup of a horizontal-flow anaerobic immobilized biomass reactor (HAIB), and examines the impact of PCP concentration on microbial diversity using denaturing gradient gel electrophoresis (DGGE). The primary mechanism for PCP removal in the HAIB was adsorption, effectively described by the Freundlich isotherm model. Adsorption efficiency ranged from 86 to 104% for PCP concentrations between 0.2 and 5.0 mg/L, and 46% to 64% for concentrations between 0.098 and 0.05 mg/L. Additionally, PCP degradation intermediates such as 2,3-DCP and 2,6-DCP were detected, indicating that biodegradation also occurred in the HAIB. Organic matter degradation averaged 81 ± 9%, and methane content in the biogas averaged 46 ± 9%, confirming the anaerobic process. No inhibition of microbial activity was observed due to PCP toxicity, even at a PCP load of 5 mg PCP/g STV per day. While the archaeal community showed only slight changes, with similarity coefficients ranging from 88 to 95%, the bacterial community was significantly affected by PCP, with similarity coefficients ranging from 18 to 50%. Bacterial groups were responsible for the initial PCP degradation, while the archaeal community was involved in metabolizing the resulting byproducts. The use of indigenous inoculum from the Santos-São Vicente estuary demonstrated its potential for effective PCP removal. Polyurethane foam proved to be an effective support material, enhancing the adsorption process and reducing PCP toxicity to the microbial consortium. This study provides valuable insights into PCP adsorption and biodegradation mechanisms in HAIB, highlighting the effectiveness of indigenous inoculum and polyurethane foam for PCP removal.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutations of methionine 444 interacting with T1Cu-coordinating amino acids affect the structure and function of multicopper oxidase CopA","authors":"Wenwei Tang,&nbsp;Peiqi Zhang,&nbsp;Xiaoyu Jin,&nbsp;Xiaorong Li,&nbsp;Shichao Chen,&nbsp;Xinping Zeng","doi":"10.1007/s10532-024-10102-4","DOIUrl":"10.1007/s10532-024-10102-4","url":null,"abstract":"<div><p>Manganese is an essential trace element for humans, animals, and plants, but excessive amounts of manganese can cause serious harm to organisms. The biological manganese oxidation process mainly oxidizes Mn(II) through the secretion of unique manganese oxidase by manganese-oxidizing bacteria. The T1 Cu site of multicopper oxidase is the main site for substrate oxidation, and its role is to transfer electrons to TNC, where dioxygen reduction occurs. In this study, methionine (Met) No. 444 interacting with the T1Cu-coordinating amino acid in the multicopper oxidase CopA from <i>Brevibacillus panacihumi</i> MK-8 was mutated to phenylalanine (Phe) and leucine (Leu) by the enzyme. Based on the analysis of enzymatic properties and the structural model, the mutant protein M444F with 4.58 times the catalytic efficiency of the original protein CopA and the mutant protein M444L with 1.67 times the catalytic efficiency of the original protein CopA were obtained. The study showed that the manganese removal rate of the manganese-oxidizing engineered bacterium Rosetta-pET-<i>copA</i>^M444L cultured for 7 days was 88.87%, which was 10.77% higher than that of the original engineered bacterium. Overall, this study provides a possibility for the application of genetic engineering in the field of biological manganese removal.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights of energy potential in thermophilic sugarcane vinasse and molasses treatment: does two-stage codigestion enhance operational performance?","authors":"Alexandre Rodrigues Ribeiro,&nbsp;Kauanna Uyara Devens,&nbsp;Franciele Pereira Camargo,&nbsp;Isabel Kimiko Sakamoto,&nbsp;Maria Bernadete Amâncio Varesche,&nbsp;Edson Luiz Silva","doi":"10.1007/s10532-024-10097-y","DOIUrl":"10.1007/s10532-024-10097-y","url":null,"abstract":"<div><p>The study evaluated the performance of thermophilic co-digestion in both single-stage methanogenic reactors (TMR) and two-stage systems, consisting of a thermophilic acidogenic reactor and a thermophilic sequential methanogenic reactor (TSMR). A 1:1 mixture of sugarcane vinasse and molasses was codigested in anaerobic fluidized bed reactors, with varying organic matter concentrations based on chemical oxygen demand (COD) ranging from 5 to 22.5 g COD L⁻¹. Both systems achieved high organic matter removal efficiency (51 to 86.5%) and similar methane (CH₄) yields (&gt; 148 mL CH₄ g⁻¹COD_removed). However, at the highest substrate concentration (22.5 g COD L⁻¹), the TSMR outperformed the TMR in terms of energy generation potential (205.6 kJ d⁻¹ vs. 125 kJ d⁻¹). Phase separation in the two-stage system increased bioenergy generation by up to 43.5% at lower substrate concentrations (7.5 g COD L⁻¹), with hydrogen (H₂) generation playing a critical role in this enhancement. Additionally, the two-stage system produced value-added products, including ethanol (2.3 g L⁻¹), volatile organic acids (3.2 g lactate L⁻¹), and H₂ (0.6–2.7 L H₂ L⁻¹ d⁻¹). Microbial analysis revealed that <i>Thermoanaerobacterium</i>, <i>Caldanaerobius</i>, and <i>Clostridium</i> were dominant at 5 g COD L⁻¹, while <i>Lactobacillus</i> prevailed at concentrations of ≥ 15 g COD L⁻¹. The primary methane producers in the single-stage system were <i>Methanosarcina</i>, <i>Methanoculleus</i>, and <i>Methanobacterium</i>, whereas <i>Methanothermobacter</i>, <i>Bathyarchaeia</i>, and <i>Methanosarcina</i> dominated in the two-stage system.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and purification of esterase enzyme from marine bacteria associated with biodegradation of polyvinyl chloride (PVC)","authors":"Shrikant D. Khandare,&nbsp;Doongar R. Chaudhary,&nbsp;Bhavanath Jha","doi":"10.1007/s10532-024-10101-5","DOIUrl":"10.1007/s10532-024-10101-5","url":null,"abstract":"<div><p>Polyvinyl chloride (PVC) is the third most produced synthetic plastic and releases the most harmful and lethal environmental component after incineration and landfilling. Few studies on microbial degradation of PVC have been reported but very little knowledge about the enzymes. In the present study, esterase enzyme was isolated and partially purified from marine bacterial isolates (T-1.3, BP-4.3 and S-237 identified as <i>Vibrio</i> sp., <i>Alteromonas</i> sp., and <i>Cobetia</i> sp., respectively) having the capability of PVC degradation. Initially, a plate assay was carried out for testing esterase production by studying bacteria using 1-naphthyl acetate as substrate. Enzyme assay showed higher production of esterase i.e. 0.57 U mL⁻¹ (2nd day), 0.46 U mL⁻¹ (2nd day) and 0.55 U mL⁻¹ (5th day) by bacterial isolate <i>Vibrio</i> sp., <i>Alteromonas</i> sp. and <i>Cobetia</i> sp., respectively incubated with PVC. Other enzymes like lipase, laccase and manganese peroxidase were much less or negligible compared to esterase enzyme production. Sephadex G-50 column purification had shown 58.62, 42.35 and 223.70 units mg⁻¹ of a specific activity by esterase for bacterial isolates <i>Vibrio</i> sp<i>., Alteromonas</i> sp<i>. and Cobetia</i> sp<i>.</i>, respectively. Further, Sephadex G-50 column purification removed all the contamination and gave a clear appearance of the band at 38, 20 and 20 KD for bacterial isolates <i>Vibrio</i> sp., <i>Alteromonas</i> sp., and <i>Cobetia</i> sp., respectively. Esterase has shown maximum stability at a range of pH between 6.0 to 7.5, temperature between 30 to 35 °C and salinity concentration between 3 to 3.5 M for all bacterial isolates. In conclusion, esterase enzyme has promising potential to degrade PVC which can contribute to the decline the plastic pollution in an eco-friendly manner from the environment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S0-dependent bio-reduction for antimonate detoxification from wastewater by an autotrophic bioreactor with internal recirculation","authors":"Dongjin Wan,&nbsp;Zhan Shen,&nbsp;Naiyuan Shi,&nbsp;Jiekai Wang,&nbsp;Weichao Zhang,&nbsp;Yahui Shi,&nbsp;Panting Wang,&nbsp;Qiaochong He","doi":"10.1007/s10532-024-10099-w","DOIUrl":"10.1007/s10532-024-10099-w","url":null,"abstract":"<div><p>Elemental sulfur (S⁰) autotrophic reduction is a promising approach for antimonate [Sb(V)] removal from water; however, it is hard to achieve effective removal of total antimony (TSb). This study established internal recirculation in an S⁰ autotrophic bioreactor (SABIR) to enhance TSb removal from Sb(V)-contaminated water. Complete Sb(V) reduction (10 mg/L) with bare residual Sb(III) (&lt; 0.26 mg/L) was achieved at hydraulic retention time (HRT) = 8 h. Shortening HRT adversely affected the removal efficiencies of Sb(V) and TSb; meanwhile, an increased reflux ratio was conducive to Sb(V) and TSb removal at the same HRT. Sulfur disproportionation occurred in the SABIR and was the primary source for SO₄²⁻ generation and alkalinity consumption. The alkalinity consumption decreased with the shortening HRT and increased with an increased reflux ratio at the same HRT. The generated SO₄²⁻ was significantly higher (50–100 times) than the theoretical value for Sb(V) reduction. Coefficient of variation (<i>CV</i>), first-order kinetic models, and osmolality analyses showed that internal recirculation did not significantly affect the stability of SABIR but contributed to enhancing TSb removal by increasing mass transfer and reflowing generated sulfide back to the SABIR. SEM–EDS, Raman spectroscopy, XRD and XPS analyses identified that the precipitates in the SABIR were Sb₂S₃ and Sb-S compounds. In addition, high-throughput sequencing analysis revealed the microbial community structure's temporal and spatial distribution in the SABIR. Dominant genera, including <i>unclassified-Proteobacteria</i> (18.72–38.99%), <i>Thiomonas</i> (0.94–4.87%) and <i>Desulfitobacterium</i> (1.18–2.75%) might be responsible for Sb(V) bio-reduction and removal. This study provides a strategy to remove Sb from water effectively and supports the theoretical basis for the practical application of the SABIR in Sb(V)-contaminated wastewater.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Enterobacter cloacae-mediated polymer biodegradation: in-silico analysis predicts broad spectrum degradation potential by Alkane monooxygenase","authors":"Mohamed Shafana Farveen,&nbsp;Rajnish Narayanan","doi":"10.1007/s10532-024-10095-0","DOIUrl":"10.1007/s10532-024-10095-0","url":null,"abstract":"","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"1009 - 1009"},"PeriodicalIF":3.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach","authors":"Mohamed Shafana Farveen,&nbsp;Thirumurthy Madhavan,&nbsp;Rajnish Narayanan","doi":"10.1007/s10532-024-10094-1","DOIUrl":"10.1007/s10532-024-10094-1","url":null,"abstract":"","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"1007 - 1007"},"PeriodicalIF":3.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a microbial community structure index (MCSI) as an approach to evaluate and optimize bioremediation performance","authors":"Jeff Gamlin,&nbsp;Renee Caird,&nbsp;Neha Sachdeva,&nbsp;Yu Miao,&nbsp;Claudia Walecka-Hutchison,&nbsp;Shaily Mahendra,&nbsp;Susan K. De Long","doi":"10.1007/s10532-024-10093-2","DOIUrl":"10.1007/s10532-024-10093-2","url":null,"abstract":"<div><p>Much attention is placed on organohalide-respiring bacteria (OHRB), such as <i>Dehalococcoides</i>, during the design and performance monitoring of chlorinated solvent bioremediation systems. However, many OHRB cannot function effectively without the support of a diverse group of other microbial community members (MCMs), who play key roles fermenting organic matter into more readily useable electron donors, producing corrinoids such as vitamin B12, or facilitating other important metabolic processes or biochemical reactions. While it is known that certain MCMs support dechlorination, a metric considering their contribution to bioremediation performance has yet to be proposed. Advances in molecular biology tools offer an opportunity to better understand the presence and activity of specific microbes, and their relation to bioremediation performance. In this paper, we test the hypothesis that a specific microbial consortium identified within 16S ribosomal ribonucleic acid (rRNA) gene next generation sequencing (NGS) data can be predictive of contaminant degradation rates. Field-based data from multiple contaminated sites indicate that increasing relative abundance of specific MCMs correlates with increasing first-order degradation rates. Based on these results, we present a framework for computing a simplified metric using NGS data, the <i>Microbial Community Structure Index</i>, to evaluate the adequacy of the microbial ecosystem during assessment of bioremediation performance.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"993 - 1006"},"PeriodicalIF":3.1,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delineating acetaminophen biodegradation kinetics and metabolomics using bacterial community","authors":"Bhavana Pandey,&nbsp;Suresh Kumar Dubey","doi":"10.1007/s10532-024-10090-5","DOIUrl":"10.1007/s10532-024-10090-5","url":null,"abstract":"<div><p>Acetaminophen [N-(4-hydroxyphenyl) acetamide, APAP] is an extensively and frequently consumed over-the-counter analgesic and antiphlogistic medication. It is being regarded as an emerging pollutant due to its continuous increment in the environment instigating inimical impacts on humans and the ecosystem. Considering its wide prevalence in the environment, there is an immense need of appropriate methods for the removal of APAP. The present study indulged screening and isolation of APAP degrading bacterial strains from pharmaceuticals-contaminated sites, followed by their molecular characterization via 16S rRNA sequencing. The phylogenetic analyses assigned the isolates to the genera <i>Pseudomonas, Bacillus, Paracoccus, Agrobacterium, Brucella, Escherichia, and Enterobacter</i> based on genetic relatedness. The efficacy of these strains in batch cultures tested through High-performance Liquid Chromatography (HPLC) revealed <i>Paracoccus</i> sp. and <i>Enterobacter</i> sp. as the most promising bacterial isolates degrading up to 88.96 and 85.92%, respectively of 300 mg L⁻¹ of APAP within 8 days of incubation. Michaelis–Menten kinetics model parameters also elucidated the high degradation potential of these isolates. The major metabolites identified through FTIR and GC–MS analyses were 4-aminophenol, hydroquinone, and 3-hydroxy-2,4-hexadienedioic. Therefore, the outcomes of this comprehensive investigation will be of paramount significance in formulating strategies for the bioremediation of acetaminophen-contaminated sites through a natural augmentation process via native bacterial strains.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"951 - 967"},"PeriodicalIF":3.1,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterobacter cloacae-mediated polymer biodegradation: in-silico analysis predicts broad spectrum degradation potential by Alkane monooxygenase","authors":"Shafana Farveen Mohamed,&nbsp;Rajnish Narayanan","doi":"10.1007/s10532-024-10091-4","DOIUrl":"10.1007/s10532-024-10091-4","url":null,"abstract":"<div><p>Plastic pollution poses a significant environmental challenge. In this study, the strain <i>Enterobacter cloacae</i> O5-E, a bacterium displaying polyethylene-degrading capabilities was isolated. Over a span of 30 days, analytical techniques including x-ray diffractometry, scanning electron microscopy, optical profilometry, hardness testing and mass spectrometric analysis were employed to examine alterations in the polymer. Results revealed an 11.48% reduction in crystallinity, a 50% decrease in hardness, and a substantial 25-fold increase in surface roughness resulting from the pits and cracks introduced in the polymer by the isolate. Additionally, the presence of degradational by-products revealed via gas chromatography ascertains the steady progression of degradation. Further, recognizing the pivotal role of alkane monooxygenase in plastic degradation, the study expanded to detect this enzyme in the isolate molecularly. Molecular docking studies were conducted to assess the enzyme’s affinity with various polymers, demonstrating notable binding capability with most polymers, especially with polyurethane (− 5.47 kcal/mol). These findings highlight the biodegradation potential of <i>Enterobacter cloacae</i> O5-E and the crucial involvement of alkane monooxygenase in the initial steps of the degradation process, offering a promising avenue to address the global plastic pollution crisis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"969 - 991"},"PeriodicalIF":3.1,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}