Biodegradation最新文献

{"title":"Anthracene detoxification by Laccases from indigenous fungal strains Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12","authors":"Samson O. Egbewale,&nbsp;Ajit Kumar,&nbsp;Tosin A. Olasehinde,&nbsp;Mduduzi P. Mokoena,&nbsp;Ademola O. Olaniran","doi":"10.1007/s10532-024-10084-3","DOIUrl":"10.1007/s10532-024-10084-3","url":null,"abstract":"<div><p>The persistence and ubiquity of polycyclic aromatic hydrocarbons (PAHs) in the environment necessitate effective remediation strategies. Hence, this study investigated the potential of purified Laccases, <i>Tl</i>FLU1L and <i>Tp</i>FLU12L, from two indigenous fungi <i>Trichoderma lixii</i> FLU1 (<i>Tl</i>FLU1) and <i>Talaromyces pinophilus</i> FLU12 (<i>Tp</i>FLU12), respectively for the oxidation and detoxification of anthracene. Anthracene was degraded with <i>v</i>_max values of 3.51 ± 0.06 mg/L/h and 3.44 ± 0.06 mg/L/h, and <i>K</i>_m values of 173.2 ± 0.06 mg/L and 73.3 ± 0.07 mg/L by <i>Tl</i>FLU1L and <i>Tp</i>FLU12L, respectively. The addition of a mediator compound 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to the reaction system significantly increased the degradation of anthracene, with up to a 2.9-fold increase in <i>v</i>_max value and up to threefold decrease in <i>K</i>_m values of <i>Tl</i>FLU1L and <i>Tp</i>FLU12L. The GC–MS analysis of the metabolites suggests that anthracene degradation follows one new pathway unique to the ABTS system—hydroxylation and carboxylation of C-1 and C-2 position of anthracene to form 3-hydroxy-2-naphthoic acid, before undergoing dioxygenation and side chain removal to form chromone which was later converted into benzoic acid and CO₂. This pathway contrasts with the common dioxygenation route observed in the free Laccase system, which is observed in the second degradation pathways. Furthermore, toxicity tests using <i>V. parahaemolyticus</i> and HT-22 cells, respectively, demonstrated the non-toxic nature of Laccase-ABTS-mediated metabolites. Intriguingly, analysis of the expression level of Alzheimer’s related genes in HT-22 cells exposed to degradation products revealed no induction of neurotoxicity unlike untreated cells. These findings propose a paradigm shift for bioremediation by highlighting the Laccase-ABTS system as a promising green technology due to its efficiency with the discovery of a potentially less harmful degradation pathway, and the production of non-toxic metabolites.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"769 - 787"},"PeriodicalIF":3.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141185842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of biofiltration for mitigating harmful gaseous emissions from small or old landfills: a review","authors":"El Farouk Omar Merouani,&nbsp;Milad Ferdowsi,&nbsp;Gerardo Buelna,&nbsp;J. Peter Jones,&nbsp;El-Hadi Benyoussef,&nbsp;Luc Malhautier,&nbsp;Michèle Heitz","doi":"10.1007/s10532-024-10082-5","DOIUrl":"10.1007/s10532-024-10082-5","url":null,"abstract":"<div><p>Landfills are widely employed as the primary means of solid waste disposal. However, this practice generates landfill gas (LFG) which contains methane (CH₄), a potent greenhouse gas, as well as various volatile organic compounds and volatile inorganic compounds. These emissions from landfills contribute to approximately 25% of the total atmospheric CH₄, indicating the imperative need to valorize or treat LFG prior to its release into the atmosphere. This review first aims to outline landfills, waste disposal and valorization, conventional gas treatment techniques commonly employed for LFG treatment, such as flares and thermal oxidation. Furthermore, it explores biotechnological approaches as more technically and economically feasible alternatives for mitigating LFG emissions, especially in the case of small and aged landfills where CH₄ concentrations are often below 3% v/v. Finally, this review highlights biofilters as the most suitable biotechnological solution for LFG treatment and discusses several advantages and challenges associated with their implementation in the landfill environment.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"469 - 491"},"PeriodicalIF":3.1,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three strategy rules of filamentous fungi in hydrocarbon remediation: an overview","authors":"Micheal Helan Soundra Rani,&nbsp;Ramesh Kumar Nandana,&nbsp;Alisha Khatun,&nbsp;Velumani Brindha,&nbsp;Durairaj Midhun,&nbsp;Ponnusamy Gowtham,&nbsp;Siva Shanmugam Dhanush Mani,&nbsp;Sivaraman Rathish Kumar,&nbsp;Anguraj Aswini,&nbsp;Sugumar Muthukumar","doi":"10.1007/s10532-024-10086-1","DOIUrl":"10.1007/s10532-024-10086-1","url":null,"abstract":"<div><p>Remediation of hydrocarbon contaminations requires much attention nowadays since it causes detrimental effects on land and even worse impacts on aquatic environments. Tools of bioremediation especially filamentous fungi permissible for cleaning up as much as conceivable, at least they turn into non-toxic residues with less consumed periods. Inorganic chemicals, CO₂, H₂O, and cell biomass are produced as a result of the breakdown and mineralization of petroleum hydrocarbon pollutants. This paper presents a detailed overview of three strategic rules of filamentous fungi in remediating the various aliphatic, and aromatic hydrocarbon compounds: utilizing carbons from hydrocarbons as sole energy, Co-metabolism manners (Enzymatic and Non-enzymatic theories), and Biosorption approaches. Upliftment in the degradation rate of complex hydrocarbon by the Filamentous Fungi in consortia scenario we can say, “Fungal Talk”, which includes a variety of cellular mechanisms, including biosurfactant production, biomineralization, and precipitation, etc., This review not only displays its efficiency but showcases the field applications – cost-effective, reliable, eco-friendly, easy to culture as biomass, applicable in both land and any water bodies in operational environment cleanups. Nevertheless, the potentiality of fungi-human interaction has not been fully understood, henceforth further studies are highly endorsed with spore pathogenicity of the fungal species capable of high remediation rate, and the gene knockout study, if the specific peptides cause toxicity to any living matters via Genomics and Proteomics approaches, before application of any in situ or ex situ environments.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"833 - 861"},"PeriodicalIF":3.1,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on microbial diversity of washing machines","authors":"Yunyi Cao,&nbsp;Shuai Yuan,&nbsp;Lingling Pang,&nbsp;Jiuyan Xie,&nbsp;Yi Gao,&nbsp;Jian Zhang,&nbsp;Zhenyao Zhao,&nbsp;Su Yao","doi":"10.1007/s10532-023-10069-8","DOIUrl":"10.1007/s10532-023-10069-8","url":null,"abstract":"<div><p>Health and environmental protection are the development trend of household appliances, coupled with the impact of the COVID-19 epidemic in the past few years. Consumers have unprecedented concerns and expectations about the sterilization and disinfection functions of household appliances. As a washing and nursing equipment for household clothes, the anti-bacterial technology of washing machine has developed rapidly. The new models of washing machines in the market have basically added the function of sterilization. In order to thoroughly solve the problem of sterilization and bacteriostasis of washing machines from the source, the distribution of microbial contamination in washing machines should be fully investigated. At present, there is almost no systematic study on the microbial community structure in washing machines in China. Therefore, the purpose of this study is to analyze the bacterial community structure in Chinese household washing machines. To explore the key factors affecting the bacterial community structure of washing machines. Bacterial communities were comprehensively analyzed by high throughput sequencing. Using chao and shannon indexes as indicators, one-way ANOVA was used to explore the key factors affecting the bacterial community structure of washing machines. A total of 2,882,778 tags and 21,265 OTUs from 522 genera were sequenced from 56 washing machine samples. Genus <i>Mycobacterium</i>, <i>Pseudomonas</i>, <i>Brevundimonas</i>, <i>Sphingomonas</i>, <i>Sphingobium</i>, <i>Enhydrobacter</i>, <i>Methylobacterium</i>, <i>Pseudoxanthomonas</i>, <i>Stenotrophomonas</i> and <i>Sphingopyxis</i> were the top ten bacteria genera in abundance. The effects of sources, types, frequency of utilization, sampling locations and service life of washing machines on bacterial diversity in washing machine were systematically analyzed. The statistical analysis showed that service life was an important factor affecting bacterial diversity in washing machine. Our study lays a foundation for directional screening of characteristic microorganisms with targeted characters including malodor-producing, fouling, pathogenic and stress-resistance, the antibacterial evaluation, metabolic mechanism of key characteristic microorganisms as well as antibacterial materials development. At present, the sterilization technology of washing machines has not been fully in combination with the distribution survey of microorganisms in washing machines. According to the specific microorganism distribution condition of the washing machine, the key distribution positions and the types of specific microorganisms contained in different positions, conduct more targeted sterilization treatment. This will help to completely solve the problem of microbial growth in washing machines from the source.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 6","pages":"819 - 831"},"PeriodicalIF":3.1,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of Pseudomonas fluorescens into soil-like fraction from municipal solid waste management park to enhance plastic biodegradation","authors":"S. Sangeetha,&nbsp;P. Hari Krishna","doi":"10.1007/s10532-024-10085-2","DOIUrl":"10.1007/s10532-024-10085-2","url":null,"abstract":"<div><p>Single-use facial masks which are predominantly made out of polypropylene is being used and littered in large quantities during post COVID-19 situation. Extensive researches on bioremediation of plastic pollution on soil led to the identification of numerous plastic degrading microorganisms. These organisms assimilate plastic polymers as their carbon source for synthesizing energy. <i>Pseudomonas fluorescens</i> (PF) is one among such microorganism which is being identified to biodegrade plastic polymers in controlled environment. The natural biodegradation of facial mask in soil-like fraction collected from municipal waste management site, bioaugmentation of the degradation process with <i>Pseudomonas fluorescens</i>, biostimulation of the soil with carbonless nutritional supplements and combined bioaugmentation with biostimulation process were studied in the present work. The study has been conducted both in controlled and in natural condition for a period of 12 months. The efficiency of the degradation was verified through FTIR analyses using carbonyl index, bond energy change, Loss in ignition (LOI) measurement along with CHNS analyses of residual substances. The analysis of results reported that carbonyl index (in terms of transmittance) was reduced to 46% of the control batch through the inclusion of PF in natural condition. The bioaugmented batch maintained in natural condition showed 33% reduction of LOI with respect to the control batch. The unburnt carbon content of the residual matter obtained from the furnace were analysed using CHNS analyser and indicated the lowest carbon content in the same bioaugmented batch. In this study, an attempt is made to verify the feasibility of enhancing biodegradation of single-use facial mask by bioaugmentation of soil-like fraction available in solid waste management park with <i>Pseudomonas fluorescens</i> under natural condition. CHNS and FTIR analysis assures the biodegradation of plastic waste in the soil-like fraction using <i>Pseudomonas fluorescens</i> under both controlled and natural environmental condition.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"789 - 802"},"PeriodicalIF":3.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140832629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of petroleum refinery wastewater by fungal stains isolated from the fishing harbour of Bizerte (Mediterranean Sea)","authors":"Tahani El Ayari,&nbsp;Rihab Bouhdida,&nbsp;Hadda Imene Ouzari,&nbsp;Najoua Trigui El Menif","doi":"10.1007/s10532-024-10083-4","DOIUrl":"10.1007/s10532-024-10083-4","url":null,"abstract":"<div><p>The study was conducted in order to explore the potential of fungi isolated from surface and bottom seawater collected from the fishing harbour of Bizerte on the bioremediation of industrial effluent (IE) contaminated by petroleum hydrocarbon. Among the 128 fungal isolates, 11 were isolated from surface seawater and 7 from bottom seawater, representing 18 taxa in total. The gas chromatography mass spectrometry (GC–MS) was used for the determination of hydrocarbon compounds in IE. An initial screening of fungal growth using six concentrations ranged between 20 and 70% (v/v) IE has allowed the identification of the optimal concentration for fungal growth as well as selection of species able to tolerate high amounts of hydrocarbon. Colorimetric test employing 2,6-dichlorophenol indophenol and gravimetric method was applied for the assessment of fungal growth using 20% EI. By checking the phylogenetic affiliation of the high-performing stains as identified using ITSr DNA sequence, a dominance of Ascomycetes was detected. Indeed, <i>Aspergillus terreus</i> and <i>Penicillium expansum</i> may degrade 82.07 and 81.76% of residual total petroleum hydrocarbon (TPH), respectively. Both species were collected from surface seawater. While, <i>Aspergillus niger</i>, <i>Colletotrichum sp</i> and <i>Fusarium annulatum</i> displayed comparable degradation rates 40.43%, 41.3%, and 42.03%, respectively. The lowest rate of degradation 33.62% was detected in <i>Emericellopsis phycophila</i>. All those species were isolated from bottom seawater, excepting <i>A. niger</i> isolated from surface water. This work highlighted the importance of exploring the potential of fungi isolated from the natural environment on the bioremediation of industrial effluent. Our results promoted the investigation of the potential of the high-performing isolates <i>A. terreus</i> and <i>P. expansum</i> on the bioremediation of IE at pilot-scale and then in situ.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"755 - 767"},"PeriodicalIF":3.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140833040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of waste engine oil to mono- and di-rhamnolipid in a sustainable approach to circular bioeconomy","authors":"Shailee Gaur,&nbsp;Mohan Jujaru,&nbsp;Revanth Vennu,&nbsp;Suresh Gupta,&nbsp;Amit Jain","doi":"10.1007/s10532-024-10081-6","DOIUrl":"10.1007/s10532-024-10081-6","url":null,"abstract":"<div><p>This study aims to valorize waste engine oil (WEO) for synthesizing economically viable biosurfactants (rhamnolipids) to strengthen the circular bioeconomy concept. It specifically focuses on investigating the influence of key bioprocess parameters, viz. agitation and aeration rates, on enhancing rhamnolipid yield in a fed-batch fermentation mode. The methodology involves conducting experiments in a stirred tank bioreactor (3 L) using <i>Pseudomonas aeruginosa</i> gi |KP 163922| as the test organism. Central composite design and response surface methodology (CCD-RSM) are employed to design the experiments and analyze the effects of agitation and aeration rates on various parameters, including dry cell biomass (DCBM), surface tension, tensoactivity, and rhamnolipid yield. It is also essential to determine the mechanistic pathway of biosurfactant production followed by the strain using complex hydrophobic substrates such as WEO. The study reveals that optimal agitation and aeration rates of 200 rpm and 1 Lpm result in the highest biosurfactant yield of 29.76 g/L with minimal surface tension (28 mN/m). Biosurfactant characterization using FTIR, ¹H NMR, and UPLC-MS/MS confirm the presence of dominant molecular ion peaks m/z 543.9 and 675.1. This suggests that the biosurfactant is a mixture of mono- and di-rhamnolipids (RhaC10C10, RhaRhaC10C12:1, RhaRhaC12:1C10). The findings present a sustainable approach for biosurfactant production in a fed-batch bioreactor. This research opens the possibility of exploring the use of pilot or large-scale bioreactors for biosurfactant production in future investigations.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"803 - 818"},"PeriodicalIF":3.1,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140656860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation of pretilachlor and fenclorim and effects of these compounds on bacterial communities under anaerobic condition","authors":"Ha Danh Duc,&nbsp;Nguyen Thi Oanh,&nbsp;Nguyen Thi Dieu Thuy,&nbsp;Nguyen Thi Kim Xuan","doi":"10.1007/s10532-024-10078-1","DOIUrl":"10.1007/s10532-024-10078-1","url":null,"abstract":"<div><p>Pretilachlor and safener fenclorim are the main components of herbicides widely applied to control weeds. Although some pure cultures of bacteria and fungi which degraded these compounds under aerobic conditions were isolated, no isolated pretilachlor- and fenclorim-degrading bacterial strains under anaerobic condition had been available. In this study, the degradation of these compounds and the effects of them on bacterial community structures were investigated under anaerobic conditions. The dissipation rates of pretilachlor and fenclorim in slurries were in the order: soil from paddy field ≈ sediment from river &gt; sediment from mangrove. Moreover, three pretilachlor-degrading bacterial strains (<i>Pseudomonas</i> sp. Pr1, <i>Proteiniclasticum</i> sp. Pr2 and <i>Paracoccus denitrificans</i> Pr3) and two fenclorim-degrading strains (<i>Dechloromonas</i> sp. Fe1 and <i>Ralstonia pickettii</i> Fe2) isolated from a slurry of paddy soil utilized the substrates as sole carbon and energy sources under anaerobic conditions. The degradation of pure pretilachlor and fenclorim at various concentrations by corresponding mixed pure cultures followed the Michaelis–Menten model, with the maximum degradation was 3.10 ± 0.31 µM/day for pretilachlor, and 2.08 ± 0.18 µM/day for fenclorim. During the degradation, 2-chloro-N-(2,6-diethylphenyl) acetamide and 2,6-dimethylaniline were produced in pretilachlor degradation, and benzene was a product of fenclorim degradation. The synergistic degradation of both substrates by all isolated bacteria reduced the metabolites concentrations accumulated in media. This study provides valuable information on effects of pretilachlor and fenclorim on bacterial communities in soil and sediments, and degradation of these substrates by isolated bacteria under anaerobic condition.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"583 - 599"},"PeriodicalIF":3.1,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140656669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia","authors":"Rosa Acevedo-Barrios,&nbsp;Irina Tirado-Ballestas,&nbsp;Angela Bertel-Sevilla,&nbsp;Leonor Cervantes-Ceballos,&nbsp;Jorge L. Gallego,&nbsp;María Angélica Leal,&nbsp;David Tovar,&nbsp;Jesús Olivero-Verbel","doi":"10.1007/s10532-024-10079-0","DOIUrl":"10.1007/s10532-024-10079-0","url":null,"abstract":"","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"601 - 620"},"PeriodicalIF":3.1,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10532-024-10079-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140609280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance, kinetic characteristics and bacterial community of short-cut nitrification and denitrification system at different ferrous ion conditions","authors":"Ben-ze Chang,&nbsp;Shuai Zhang,&nbsp;Dong-zhi Chen,&nbsp;Kai-tuo Gao,&nbsp;Guang-feng Yang","doi":"10.1007/s10532-024-10080-7","DOIUrl":"10.1007/s10532-024-10080-7","url":null,"abstract":"<div><p>In order to explore the operation performance, kinetic characteristics and bacterial community of the short-cut nitrification and denitrification (SND) system, the SND system with pre-cultured short cut nitrification and denitrification sludge was established and operated under different ferrous ion (Fe (II)) conditions. Experimental results showed that the average NH₄⁺–N removal efficiency (ARE) of SND system was 97.3% on Day 5 and maintained a high level of 94.9% ± 1.3% for a long operation period. When the influent Fe(II) concentration increased from 2.3 to 7.3 mg L⁻¹, the sedimentation performance, sludge concentration and organic matter removal performance were improved. However, higher Fe(II) of 12.3 mg L⁻¹ decreased the removal of nitrogen and COD_Cr with the relative abundance (RA) of Proteobacteria and Bacteroidetes decreased to 30.28% and 19.41%, respectively. Proteobacteria, Bacteroidetes and Firmicutes were the dominant phyla in SND system. Higher Fe(II) level of 12.3 mg L⁻¹ increase the RA of denitrifying genus <i>Trichococcus</i> (33.93%), and the denitrifying genus <i>Thauera</i> and <i>Tolumonas</i> dominant at Fe(II) level of no more than 7.3 mg L⁻¹.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"35 5","pages":"621 - 639"},"PeriodicalIF":3.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}