Advanced Healthcare Materials最新文献

{"title":"Shelf-Stable Human-Derived Granular Extracellular Matrix (gECM) Hydrogel Wafers as 2.5D Substrates for Microphysiological Modeling.","authors":"Juliet O Heye, Shannon A Blanco, Stephanie E Schneider, Shantae Gallegos, Jeanne E Barthold, Michael Floren, Victor Acosta, Stéphane Avril, Corey P Neu","doi":"10.1002/adhm.71916","DOIUrl":"https://doi.org/10.1002/adhm.71916","url":null,"abstract":"<p><p>Understanding disease pathology and evaluating emerging therapeutics require in vitro models that accurately recapitulate human tissue environments. However, existing microphysiological systems often compromise either biomimicry or ease-of-use, limiting widespread adoption and scalability. Here, we present lyophilized granular extracellular matrix (gECM) hydrogel wafers as shelf-stable, humanized 2.5D substrates that enable physiologically relevant modeling while simplifying integration into experimental workflows. Derived from decellularized human cartilage and bone, our gECM hydrogel wafers retain tissue-specific architecture while introducing microporosity and surface topography through lyophilization. These wafers maintain swelling behavior, structural integrity, and mechanical properties over three months of room-temperature storage, allowing pre-fabrication and on-demand use without loss of function. gECM hydrogel wafers support direct cell seeding without encapsulation and sustain viability and proliferation of human adipose-derived mesenchymal stromal cells over 21 days. Furthermore, wafers can be readily integrated into proof-of-concept on-chip fabrication with in situ hydration and transport of large biomolecules. Together, this platform bridges the gap between conventional 2D culture ease-of-use and 3D biomaterial biomimicry, providing a scalable, physiologically-relevant, and humanized in vitro model approach that shows potential for future high-throughput disease studies and therapeutic screening.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149369719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An All-Natural Multifunctional Hydrogel Incorporating Carrier-Free Self-Assembled Microparticles for the Treatment of S. aureus-Infected Wounds.","authors":"Mianhong Chen, Xiaobing Huang, Jizhen Zhang, David Julian McClements, Kefeng Wu, Yunxia He, Ruyi Li, Jihua Li, Wei Zhou","doi":"10.1002/adhm.71959","DOIUrl":"https://doi.org/10.1002/adhm.71959","url":null,"abstract":"<p><p>Multifunctional wound dressings combining antibacterial activity and tissue regeneration are highly desirable for infected wound management. In this study, a multifunctional chitosan-gelatin hydrogel incorporating carrier-free self-assembled anisaldehyde-tannic acid-Zn² ⁺ (AA-TA-Zn² ⁺) bioactive microparticles is developed. These microparticles are fabricated through non-covalent self-assembly and metal-phenolic coordination without additional polymeric carriers or surfactants. Anisaldehyde (AA) serves as both an antibacterial agent and a hydrophobic structural building block, while tannic acid (TA) and Zn² ⁺ provide complementary antioxidant and coordination functions. Incorporation of these microparticles into the chitosan-gelatin matrix enhances the structural integrity and multifunctionality of the resulting composite hydrogel through dynamic intermolecular interactions. The hydrogel exhibits excellent injectability, self-healing capability, tissue adhesion, and sustained release with pH-responsive behavior. In vitro experiments demonstrate the antibacterial, antioxidant, and anti-inflammatory activities of the microparticles and composite hydrogel, including their ability to alleviate oxidative stress and inflammatory responses, as well as the hydrogel's effectiveness in disrupting established Staphylococcus aureus biofilms. In a murine model of S. aureus-infected wounds, the hydrogel significantly accelerates wound closure and enhances collagen deposition and tissue restoration. This study presents a promising strategy for integrating carrier-free bioactive microparticles into a natural biopolymer hydrogel matrix, offering a multifunctional platform for infected wound management.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149369718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Non-Cryogenic Dugout-Structured Microneedle Patch for Co-Delivery of Irisin and Fibroblasts in Diabetic Wound Repair (Adv. Healthcare Mater. 41/2026)","authors":"Yu-Chi Pan,&nbsp;Hsi-Chen Tung,&nbsp;Ying-Tzu Chen,&nbsp;Hung-Pei Tsai,&nbsp;Chien-Ju Lin,&nbsp;Shu-Hung Huang,&nbsp;Hung-Wei Yang","doi":"10.1002/adhm.71763","DOIUrl":"https://doi.org/10.1002/adhm.71763","url":null,"abstract":"<p><b>Cell-Delivery Microneedles</b></p><p>A dugout-structured microneedle patch carries live fibroblasts together with irisin. A portable vacuum pump draws the cell-laden alginate into three internal microchannels within minutes, so no freezing or cryoprotectants are needed. Pressed onto a diabetic wound, the patch releases both payloads beneath the surface, lowering TNF-α, raising fibronectin and TGF-β1, and closing wounds faster than topical treatment. More details can be found in the Research Article by Shu-Hung Huang, Hung-Wei Yang, and co-workers (DOI: 10.1002/adhm.71598).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.71763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein-Polymer Dynamic Hydrogels Utilizing Native Ligand Binding for Injectable Therapeutic Delivery and 3D Printing","authors":"S. Cem Millik,&nbsp;Yonghui Wang,&nbsp;Kefan Song,&nbsp;Jessica M. Snyder,&nbsp;LeAnn Le,&nbsp;Lauren G. Brown,&nbsp;Shanny Hsuan Kuo,&nbsp;Ashleigh B. Theberge,&nbsp;Suzie H. Pun,&nbsp;Alshakim Nelson","doi":"10.1002/adhm.71810","DOIUrl":"10.1002/adhm.71810","url":null,"abstract":"<p>Dynamic hydrogels comprising networks formed from reversible interactions have shown utility in minimally invasive delivery of encapsulated therapeutics, as well as in extrusion-based 3D printing of tissue-mimetic structures. Here, we report dynamic nanocolloidal hydrogels formed from the co-assembly of spheroidal hyperbranched poly(glycerol) (HPG) and soluble albumin nanoparticles (SANs). Functionalization of the HPG with palmitate (a natural ligand of serum albumin) appears to enable self-association of the HPG, as well as its interaction with SANs, to form dynamic networks. The properties of these networks can be tuned by modifying the size of the SAN in addition to other formulation parameters. To highlight the utility of these dynamic hydrogels, we demonstrate (i) in vitro and in vivo release of a model protein therapeutic from these injectable materials, (ii) preliminarily evaluate biocompatibility of these materials in a murine model, and (iii) demonstrate extrusion-based 3D printing using these materials.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.71810","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic Nano-Assembly of Red-Blood-Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles","authors":"Chiranth K. Nagaraj,&nbsp;Xilal Y. Rima,&nbsp;Kim T. Nguyen,&nbsp;Courtney Culkins,&nbsp;Nana Boateng,&nbsp;Jacob Doon-Ralls,&nbsp;Alejandro Bresolin,&nbsp;Xin Huang,&nbsp;Hong Li,&nbsp;Vahedi Amid,&nbsp;Ajeet Pal Singh,&nbsp;Dharti Shantaram,&nbsp;Anastasiia Amari,&nbsp;Mangesh D. Hade,&nbsp;Nicholas R. Merriam,&nbsp;Zachary D. Schultz,&nbsp;Scott Q. Harper,&nbsp;Nizar Y. Saad,&nbsp;Setty M. Magaña,&nbsp;Willa A. Hsueh,&nbsp;Rachel E. Kopec,&nbsp;Blaise R. Kimmel,&nbsp;Andre F. Palmer,&nbsp;Eduardo Reátegui","doi":"10.1002/adhm.202504351","DOIUrl":"10.1002/adhm.202504351","url":null,"abstract":"<p>A central challenge in nanomedicine is the scalable and precise engineering of nanocarriers that are simultaneously tunable, immune-compatible, and capable of encapsulating diverse bioactive cargos. Extracellular vesicles (EVs), including native red blood cell-derived EVs (RBCEVs), offer biocompatibility but remain limited by heterogeneous composition, low yield, poor scalability, and limited control in cargo loading. Here, we introduce a bottom-up strategy for developing engineered red blood cell extracellular vesicles (eRBCEVs) through microfluidic diffusional mixing of purified RBC lipids and components. Parametric optimization through multi-physics simulation enabled predictive control over flow rates, lipid concentration, and channel geometry. eRBCEVs exhibited comparable encapsulation efficiency across molecular cargos of distinct sizes and properties, including oligonucleotides, gold nanoparticles, hemoglobin, erythrocruorin (∼3.6 MDa), and full adeno-associated virus (AAV ∼25 nm) particles, validated through cryo-EM, chemical mapping, and high-resolution TIRFM. Surface conjugation with CD47 peptide was established for comparison with RBCEVs; the bio-conjugations with α-PD-L1 antibodies yielded functionalized vesicles with uptake in PD-L1-positive human tumor organoids. In vivo biodistribution and pharmacokinetic analysis in mice demonstrated sustained circulation and tissue distribution in the liver, spleen, and lungs. Neutrophil activation and macrophage uptake assays confirmed minimal immunogenicity compared to conventional particles and free-protein controls. Collectively, our platform enables the scalable synthesis of customizable eRBCEVs that preserve RBC lipid features, support cargo flexibility, and potential for scale-up. This work establishes eRBCEVs as a next-generation therapeutic platform for programmable, patient-specific delivery in gene therapy, immunomodulation, and translational nanomedicine.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202504351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic Nano-Assembly of Red-Blood-Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles (Adv. Healthcare Mater. 41/2026)","authors":"Chiranth K. Nagaraj,&nbsp;Xilal Y. Rima,&nbsp;Kim T. Nguyen,&nbsp;Courtney Culkins,&nbsp;Nana Boateng,&nbsp;Jacob Doon-Ralls,&nbsp;Alejandro Bresolin,&nbsp;Xin Huang,&nbsp;Hong Li,&nbsp;Vahedi Amid,&nbsp;Ajeet Pal Singh,&nbsp;Dharti Shantaram,&nbsp;Anastasiia Amari,&nbsp;Mangesh D. Hade,&nbsp;Nicholas R. Merriam,&nbsp;Zachary D. Schultz,&nbsp;Scott Q. Harper,&nbsp;Nizar Y. Saad,&nbsp;Setty M. Magaña,&nbsp;Willa A. Hsueh,&nbsp;Rachel E. Kopec,&nbsp;Blaise R. Kimmel,&nbsp;Andre F. Palmer,&nbsp;Eduardo Reátegui","doi":"10.1002/adhm.71762","DOIUrl":"https://doi.org/10.1002/adhm.71762","url":null,"abstract":"<p><b>Red Blood Cell Lipids</b></p><p>The cover illustrates the microfluidic production of engineered red blood cell extracellular vesicle (eRBCEVs) from RBC lipids. The central vesicle encapsulates adeno-associated virus (AAV), highlighting efficient viral loading and immune-evasive delivery. The fluorescent cellular background represents successful eGFP-AAV9 transduction of HEK293T cells despite the presence of neutralizing AAV9 antibodies. More details can be found in the Research Article by Eduardo Reátegui, and co-workers (DOI: 10.1002/adhm.202504351).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.71762","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchically Structured Microparticles Encapsulated With Multiple Plant-Derived Bioactives for Psoriasis Therapy","authors":"Dingyuan Chen,&nbsp;Minhui Lu,&nbsp;Yuanjin Zhao","doi":"10.1002/adhm.71803","DOIUrl":"10.1002/adhm.71803","url":null,"abstract":"<div>\u0000 \u0000 <p>Traditional Chinese medicine bioactives have considerable therapeutic value in psoriasis management, while their clinical applications are still constrained by the insufficient therapeutic efficacy of single components, uncontrolled release, and low local bioavailability. In this study, we report a hierarchically structured microparticle system loaded with multiple plant-derived bioactives for psoriasis therapy. The microparticles of sodium alginate (SA) hydrogel with polydopamine (PDA)-modification were fabricated through microfluidics, co-loaded with paeoniflorin (PF) and ginger-derived exosome-like nanoparticles (GELNs). Benefiting from the porous structure of hydrogel and adhesive property of PDA, the microparticles enable stable co-encapsulation of multiple plant-derived bioactives and achieve sustained release at lesion sites, enhancing local bioavailability. The encapsulated PF and GELNs exert complementary anti-inflammatory, antioxidant, and anti-proliferative effects, collectively regulating key pathological processes associated with psoriasis. These results prove that the microparticle-based multiple plant-derived bioactives delivery system provides a safe, cost-effective, and translatable approach for long-term topical treatment of psoriasis, highlighting their therapeutic potential in inflammatory skin diseases.</p>\u0000 </div>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDNF-Enriched Small Extracellular Vesicle-Loaded ROS-Scavenging Hydrogel Promotes Neural Regeneration After Spinal Cord Injury","authors":"Xingyu Zhu,&nbsp;Zhong Liu,&nbsp;Kewei Li,&nbsp;Yuhong Fan,&nbsp;Kangjia Yang,&nbsp;Xiaopeng Sun,&nbsp;Hang Shi,&nbsp;Lixuan Sun,&nbsp;Yang Lou,&nbsp;Min Wang,&nbsp;Peipei Wu,&nbsp;Hui Qian,&nbsp;Qiang Fu,&nbsp;Hua Ding","doi":"10.1002/adhm.71806","DOIUrl":"10.1002/adhm.71806","url":null,"abstract":"<p>Spinal cord injury (SCI) is a severe traumatic disorder of the central nervous system that results in substantial motor and sensory dysfunction. The hostile post-injury microenvironment, marked by excessive oxidative stress, inflammatory responses, and progressive neuronal degeneration, greatly limits neural repair. In this study, we developed a multifunctional reactive oxygen species (ROS)-scavenging hydrogel composed of N-acryloylglycylamide (NAGA), xanthan gum (XG), carbomer 940 (CBP940), and tannic acid (TA) (termed NXCT) to locally deliver glial cell line-derived neurotrophic factor (GDNF)-enriched small extracellular vesicles (sEVs). GDNF, as a key neurotrophic protein, was incorporated into engineered sEVs to enhance their neuroregenerative activity. The resulting GDNF-sEVs@NXCT hydrogel exhibited favorable antioxidant and anti-inflammatory properties in vitro and effectively supported neuronal survival and axonal regeneration. After in situ implantation into the injured spinal cord, GDNF-sEVs@NXCT reduced ROS accumulation, alleviated inflammatory responses, promoted neural and myelin regeneration, and enhanced motor functional recovery in SCI rats. RNA-sequencing further uncovered that these therapeutic effects of GDNF-sEVs@NXCT were associated with PI3K/AKT pathway activation. Overall, these results demonstrate that the GDNF-sEVs@NXCT hydrogel provides a bioactive macromolecule-based therapeutic strategy for SCI repair via microenvironment modulation and neuroregeneration, highlighting its translational potential for spinal cord repair.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.71806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection-Phase Biomolecular Coronas in Inhaled Nanomedicine","authors":"Zhenchao Wu,&nbsp;Taikang Yao,&nbsp;Ning Shen","doi":"10.1002/adhm.71853","DOIUrl":"10.1002/adhm.71853","url":null,"abstract":"<div>\u0000 \u0000 <p>An inhaled nanomedicine encounters two successive forms of remodeling: device-related changes during aerosol generation and biological conditioning after deposition. Respiratory infection adds a moving interface, as epithelial secretion, surfactant composition, plasma leakage, and neutrophil-derived material vary across airway compartments and over time. Pulmonary and disease-corona studies already establish that host environments influence nanoparticle identity. The next question is how this knowledge should change formulation selection and dosing for infected lungs. We propose an infection-phase framework based on measured, overlapping airway environments. It connects the post-aerosol particle to its surface-associated biomolecules, distinguishes this interface from aggregation and bulk mucus effects, and links analytical measurements to functional delivery. A staged research program begins with one compartment and two contrasting environments, then adds controlled particle-transfer experiments, candidate-component perturbation, and donor-level validation. Lipid nanoparticles, polymeric carriers, and nanozymes require different functional endpoints within this shared framework. The immediate translational output is a biorelevant performance stress test that identifies robust or environment-selective formulations and guides the next experiment.</p>\u0000 </div>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Non-Cryogenic Dugout-Structured Microneedle Patch for Co-Delivery of Irisin and Fibroblasts in Diabetic Wound Repair","authors":"Yu-Chi Pan,&nbsp;Hsi-Chen Tung,&nbsp;Ying-Tzu Chen,&nbsp;Hung-Pei Tsai,&nbsp;Chien-Ju Lin,&nbsp;Shu-Hung Huang,&nbsp;Hung-Wei Yang","doi":"10.1002/adhm.71598","DOIUrl":"10.1002/adhm.71598","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetic wounds remain difficult to treat due to persistent inflammation and impaired fibroblast function, which slow tissue repair. Microneedle systems provide a minimally invasive route for localized delivery, but live-cell applications have been constrained by dehydration damage, cryogenic handling requirements, and complex fabrication processes. In this study, we developed a dugout-structured microneedle patch (DSMNP) for non-cryogenic co-delivery of recombinant irisin and fibroblasts. Using a tri-channel architecture together with a portable vacuum-assisted loading method, the DSMNP achieved efficient loading of cell-containing formulations without freeze-thaw cycles or cryoprotectants. In vitro, payloads were distributed throughout the microchannels to a depth of approximately 900 µm, with fibroblasts released in a time-dependent manner under cell-compatible conditions. Irisin showed good cytocompatibility with fibroblasts, enhanced fibroblast migration, and modulated early NF-κB and Akt signaling, evidenced by decreased p-IκBα/p-NF-κB levels and increased Akt phosphorylation. In a <i>streptozotocin</i> (STZ)-induced diabetic wound model, DSMNP-mediated co-delivery accelerated wound closure compared with topical application of the same payloads. Molecular and histological assessments revealed reduced TNF-α expression, elevated fibronectin, TGF-β1, and collagen deposition, and decreased α-SMA levels. Together, these findings position the DSMNP as a practical, non-cryogenic platform for delivering cells and bioactive proteins in diabetic wound healing.</p>\u0000 </div>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"15 41","pages":""},"PeriodicalIF":11.0,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}