Advanced Healthcare Materials最新文献

Natural Extracellular Matrix Scaffold-Based Hydrogel Corneal Patch with Temperature and Light-Responsiveness for Penetrating Keratoplasty and Sutureless Stromal Defect Repair. 基于天然细胞外基质支架的水凝胶角膜贴片具有温度和光反射性，可用于穿透性角膜移植术和无缝线基质缺损修复术。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-19 DOI: 10.1002/adhm.202402567

Long Zhao, Zhen Shi, Jingting Wang, Shengqian Dou, Xiuli Sun, Shang Yang, Hongwei Wang, Qingjun Zhou, Ting Wang, Weiyun Shi

{"title":"Natural Extracellular Matrix Scaffold-Based Hydrogel Corneal Patch with Temperature and Light-Responsiveness for Penetrating Keratoplasty and Sutureless Stromal Defect Repair.","authors":"Long Zhao, Zhen Shi, Jingting Wang, Shengqian Dou, Xiuli Sun, Shang Yang, Hongwei Wang, Qingjun Zhou, Ting Wang, Weiyun Shi","doi":"10.1002/adhm.202402567","DOIUrl":"https://doi.org/10.1002/adhm.202402567","url":null,"abstract":"Corneal transplantation remains the gold standard for treating corneal blindness; however, it is hampered globally by donor shortages and the complexity of suture-dependent procedures. Tissue-engineered corneas have demonstrated potential as corneal equivalents. Nevertheless, the development of adhesive corneal patches and full-thickness corneal substitutes remains challenging. In this study, a multifunctional hydrogel corneal patch (MHCP) is constructed by integrating a dual-crosslinked hybrid hydrogel with temperature and light responsiveness with a natural extracellular matrix scaffold. When applied to the ocular surface, MHCP spontaneously releases adhesives at body temperature and forms a stable adhesion with the recipient cornea through photocuring. In addition to its inherent mechanical, optical, and ultrastructural characteristics, which are similar to those of the natural stroma, MHCP demonstrates excellent suture resistance, anti-swelling, and anti-degradation properties after curing. MHCP promotes the proliferation and migration of corneal epithelial cells in vitro and maintains the phenotype of corneal stromal cells. In vivo, MHCP maintains graft hydration and restores corneal structural integrity and transparency during penetrating keratoplasty of various sizes and sutureless lamellar keratoplasty. Collectively, given the advantages of native stroma-like characteristics, operation-facilitating multiple functions, and convenient preparation, MHCP is a promising corneal substitute for clinical applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402567"},"PeriodicalIF":10.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-Gen Poly(ε-Caprolactone) Scaffolds: Non-Destructive In Vivo Monitoring and Accelerated Biodegradation. 新一代聚（ε-己内酯）支架：无损体内监测和加速生物降解。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-19 DOI: 10.1002/adhm.202402256

Kristyna Kolouchova, Quinten Thijssen, Ondrej Groborz, Lana Van Damme, Jana Humajova, Petr Matous, Astrid Quaak, Martin Dusa, Jan Kucka, Ludek Sefc, Martin Hruby, Sandra Van Vlierberghe

{"title":"Next-Gen Poly(ε-Caprolactone) Scaffolds: Non-Destructive In Vivo Monitoring and Accelerated Biodegradation.","authors":"Kristyna Kolouchova, Quinten Thijssen, Ondrej Groborz, Lana Van Damme, Jana Humajova, Petr Matous, Astrid Quaak, Martin Dusa, Jan Kucka, Ludek Sefc, Martin Hruby, Sandra Van Vlierberghe","doi":"10.1002/adhm.202402256","DOIUrl":"https://doi.org/10.1002/adhm.202402256","url":null,"abstract":"Poly(ɛ-caprolactone) (PCL) is a biocompatible, biodegradable, and highly mechanically resilient FDA-approved material (for specific biomedical applications, e.g. as drug delivery devices, in sutures, or as an adhesion barrier), rendering it a promising candidate to serve bone tissue engineering. However, in vivo monitoring of PCL-based implants, as well as biodegradable implants in general, and their degradation profiles pose a significant challenge, hindering further development in the tissue engineering field and subsequent clinical adoption. To address this, photo-cross-linkable mechanically resilient PCL networks are developed and functionalized with a radiopaque monomer, 5-acrylamido-2,4,6-triiodoisophthalic acid (AATIPA), to enable non-destructive in vivo monitoring of PCL-based implants. The covalent incorporation of AATIPA into the crosslinked PCL networks does not significantly affect their crosslinking kinetics, mechanical properties, or thermal properties, but it increases their hydrolysis rate and radiopacity. Complex and porous 3D designs of radiopaque PCL networks can be effectively monitored in vivo. This work paves the way toward non-invasive monitoring of in vivo degradation profiles and early detection of potential implant malfunctions.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402256"},"PeriodicalIF":10.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Sprayed Hydrogel Delivers Extracellular Vesicles Derived from Human Endometrial Organoids for Uterine Function Preservation and Fertility Restoration. 原位喷涂水凝胶可输送从人类子宫内膜器官组织中提取的细胞外囊泡，用于保留子宫功能和恢复生育能力。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-19 DOI: 10.1002/adhm.202403604

Xunsi Qin, Kai-Lun Hu, Qi Li, Yuze Sun, Tianliu Peng, Xiyao Liu, Jizhou Li, Wenhui Nan, Yang Yu, Xiangbing Qi, Rong Li

{"title":"In Situ Sprayed Hydrogel Delivers Extracellular Vesicles Derived from Human Endometrial Organoids for Uterine Function Preservation and Fertility Restoration.","authors":"Xunsi Qin, Kai-Lun Hu, Qi Li, Yuze Sun, Tianliu Peng, Xiyao Liu, Jizhou Li, Wenhui Nan, Yang Yu, Xiangbing Qi, Rong Li","doi":"10.1002/adhm.202403604","DOIUrl":"https://doi.org/10.1002/adhm.202403604","url":null,"abstract":"Impaired endometrial function and reduced receptivity remain significant causes of female infertility. Here, a sprayable hydrogel combined with human endometrial organoid extracellular vesicles (HEO-EVs) is developed to enhance uterine function preservation and fertility restoration. The peptide amphiphile hydrogel (labeled CPA) is engineered by conjugating a collagen-binding peptide with glutathione to impart its biocompatible adhesive and antioxidant properties. The therapeutic EVs are isolated and purified from human endometrial organoids that have been stably passaged long-term using a bioreactor-culture system. The resulting HEO-EVs-loaded CPA (CPA@HEO-EVs) rapid gelation, triggered by salt-ion interactions, occurs when the fluid is sprayed onto the uterine lining. The ex vivo studies demonstrate that CPA@HEO-EVs promote cell proliferation, scavenges free radicals, and increases tube formation in human umbilical vein endothelial cells. In vivo experiments further validate that in situ spraying with the CPA@HEO-EVs can promote neovascularization, prevent localized endometrial fibrosis, and effectively enhance fertility in a mouse model of endometrial injury. These findings highlight the promising clinical application of in situ sprayed CPA@HEO-EVs hydrogel for targeted endometrial therapy.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403604"},"PeriodicalIF":10.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel-Forming Microneedles and Applications in Interstitial Fluid Diagnostic Devices. 水凝胶成型微针及其在间质液诊断设备中的应用。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-19 DOI: 10.1002/adhm.202401782

Angélica F Aroche, Hannah E Nissan, Michael A Daniele

{"title":"Hydrogel-Forming Microneedles and Applications in Interstitial Fluid Diagnostic Devices.","authors":"Angélica F Aroche, Hannah E Nissan, Michael A Daniele","doi":"10.1002/adhm.202401782","DOIUrl":"https://doi.org/10.1002/adhm.202401782","url":null,"abstract":"Hydrogel-forming microneedles are constructed from or coated with polymeric, hydrophilic materials that swell upon insertion into the skin. Designed to dissolve or disintegrate postinsertion, these microneedles can deliver drugs, vaccines, or other therapeutics. Recent advancements have broadened their application scope to include the collection, transport, and extraction of dermal interstitial fluid (ISF) for medical diagnostics. This review presents a brief introduction to the characteristics of dermal ISF, methods for extraction and sampling, and critical assessment of the state-of-the-art in hydrogel-forming microneedles for ISF diagnostics. Key factors are evaluated including material composition, swelling behavior, biocompatibility, and mechanical strength necessary for effective microneedle performance and ISF collection. The review also discusses successful examples of dermal ISF assays and microneedle sensor integrations, highlighting notable achievements, identifying research opportunities, and addressing challenges with potential solutions. Despite the predominance of synthetic hydrogels in reported hydrogel-forming microneedle technologies due to their favorable swelling and gelation properties, there is a significant variety of biopolymers and composites reported in the literature. The field lacks consensus on the optimal material, composition, or fabrication methods, though emerging evidence suggests that processing and fabrication techniques are critical to the performance and utility of hydrogel-forming microneedles for ISF diagnostics.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2401782"},"PeriodicalIF":10.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Photothermal Therapy under Low-Power Near-Infrared Irradiation Enabled by a Si-Cyclopentadithiophene-Based Organic Molecule. 一种基于硅-环戊二烯噻吩的有机分子在低功率近红外照射下增强了光热疗法。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-18 DOI: 10.1002/adhm.202403248

Kexin Fan, Qingyang Li, Yuping Qian, Ludan Zhang, Di Lu, Ling Zhu, Shouke Yan, Bowei Xu, Yuguang Wang

{"title":"Enhanced Photothermal Therapy under Low-Power Near-Infrared Irradiation Enabled by a Si-Cyclopentadithiophene-Based Organic Molecule.","authors":"Kexin Fan, Qingyang Li, Yuping Qian, Ludan Zhang, Di Lu, Ling Zhu, Shouke Yan, Bowei Xu, Yuguang Wang","doi":"10.1002/adhm.202403248","DOIUrl":"https://doi.org/10.1002/adhm.202403248","url":null,"abstract":"Due to the inadequate photothermal conversion efficiency (PCE), most photothermal agents (PTAs) have to be used under high-power near-infrared (NIR) irradiation, which significantly exceeds medical safety standards, for achieving effective photothermal therapy (PTT) in antitumor treatment. This significantly hinders practical PTT application. Herein, three acceptor-donor-acceptor(A-D-A)-type molecules are synthesized based on cyclopentadithiophene unit to develop effective PTAs. By incorporating the large-size Si atom in the A-D-A molecules, the photosensitizer displays an increased packing distance in the aggregate state, leading to a blue-shifted absorption spectrum that better matches the medial laser wavelength. Also, the Si incorporation strategy elevates the nonradiative decay rate constants (knr) of the A-D-A photosensitizer, and thereby a further enhancement in PCE is achieved for the PTA. Consequently, the SiO-4F-based nanoparticles exhibited 64.23% PCE, with excellent biosafety and photothermal stability. Under NIR irradiation with medical safety (808 nm, 0.33 W cm-2), SiO-4F nanoparticles with 100 µg mL-1 yield a death rate of over 91% for diverse tumor cells. Moreover, in vivo experiments, SiO-4F-based PTT effectively inhibited and eliminated tumors. These findings suggest that the Si-incorporated CDPT is promising for constructing effective A-D-A photosensitizers, enabling the PTT under NIR irradiation that meets medical safety standards.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403248"},"PeriodicalIF":10.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cartilage Endplate-Targeted Engineered Exosome Releasing and Acid Neutralizing Hydrogel Reverses Intervertebral Disc Degeneration. 软骨内板靶向工程外泌体释放和酸中和水凝胶可逆转椎间盘退变

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-18 DOI: 10.1002/adhm.202403315

Jiawen Zhan, Yongzhi Cui, Ping Zhang, Yuxuan Du, Prisca Hecker, Shuaiqi Zhou, Yupeng Liang, Weiye Zhang, Zhefeng Jin, Yuan Wang, Weihang Gao, Oleksandr Moroz, Liguo Zhu, Xiaoguang Zhang, Ke Zhao

{"title":"Cartilage Endplate-Targeted Engineered Exosome Releasing and Acid Neutralizing Hydrogel Reverses Intervertebral Disc Degeneration.","authors":"Jiawen Zhan, Yongzhi Cui, Ping Zhang, Yuxuan Du, Prisca Hecker, Shuaiqi Zhou, Yupeng Liang, Weiye Zhang, Zhefeng Jin, Yuan Wang, Weihang Gao, Oleksandr Moroz, Liguo Zhu, Xiaoguang Zhang, Ke Zhao","doi":"10.1002/adhm.202403315","DOIUrl":"https://doi.org/10.1002/adhm.202403315","url":null,"abstract":"Cartilage endplate cell (CEPC) and nucleus pulposus cell (NPC) inflammation are critical factors that contribute to intervertebral disc degeneration (IVDD). Recent evidence indicated that iron ion influx, reactive oxygen species (ROS), and the cGAS-STING pathway are involved in CEPC inflammatory degeneration. Moreover, cytokines produced by degenerating CEPCs and lactic acid accumulation within the microenvironment significantly contribute to NPC inflammation. Consequently, simultaneous alleviation of CEPC inflammation and correction of the acidic microenvironment are anticipated to reverse IVDD. Herein, CEPC-targeted engineered exosomes loaded with salvianolic acid A are incorporated into a CaCO3/chitosan hydrogel, forming a composite gel, CAP-sEXOs@Gel. Notably, CAP-sEXOs@Gel shows long local retention, realizes the slow release of CAP-sEXOs and specific uptake by CEPCs. After uptake by CEPCs, CAP-sEXOs reduce intracellular iron ion and ROS by inhibiting hypoxia-inducible factor-2α (HIF-2α)/TfR1 expression. Iron ion influx and ROS inhibition contribute to the maintenance of normal mitochondrial function and reduced mtDNA leakage, suppresing the cGAS-STING pathway. Additionally, the CaCO3 component of CAP-sEXOs@Gel neutralizes H+, thereby alleviating NPC inflammation. Collectively, this novel composite hydrogel demonstrates the ability to concurrently inhibit CEPC and NPC inflammation, thereby presenting a promising therapeutic approach for IVDD.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403315"},"PeriodicalIF":10.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting Tumor Accumulation of Phthalocyanine through Sialylation Engineering for Superior Cancer Phototherapy. 通过 Sialylation 工程促进酞菁在肿瘤中的积累，实现卓越的癌症光疗。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-18 DOI: 10.1002/adhm.202402381

Jian Li, Tingting Wang, Cijuan Li, Xun Zhang, Jingchao Li, Dongsheng Zhang, Yafei Zhang, Jun Yang, Xinhui Su, Nian Liu

{"title":"Boosting Tumor Accumulation of Phthalocyanine through Sialylation Engineering for Superior Cancer Phototherapy.","authors":"Jian Li, Tingting Wang, Cijuan Li, Xun Zhang, Jingchao Li, Dongsheng Zhang, Yafei Zhang, Jun Yang, Xinhui Su, Nian Liu","doi":"10.1002/adhm.202402381","DOIUrl":"https://doi.org/10.1002/adhm.202402381","url":null,"abstract":"Phthalocyanines (Pcs) are widely developed in cancer phototherapy due to their definite chemical structure and tunable photosensitivity. However, their in vivo application is hampered by low water solubility and non-specific biodistribution. Here,a strategy of sialylation-modulation is developed for the first time to highly improve the bioavailability of Pcs. The sialylated Pcs (ZnPc-4SA) not only has good hydrophilicity, but also can self-assemble into nanoparticles (ZnPc-4SA NPs). These nanoformulations retain the excellent photophysical properties of Pcs, which in turn reflects excellent optoacoustic and phototherapeutic properties. Importantly, ZnPc-4SA NPs exhibit boosted tumor accumulation due to the passive targeting and sialic acid-mediated E-selectin targeting. Besides, the phototoxicity of ZnPc-4SA NPs can effectively trigger cell apoptosis and tumor elimination upon laser irradiation. Therefore, sialylation engineering strategy provides a new option for hydrophobic drugs modification with enhanced tumor theranostics.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402381"},"PeriodicalIF":10.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size-Tunable Boron Nanoreactors for Boron Neutron Capture Synergistic Chemodynamic Therapy of Tumor. 用于硼中子俘获的尺寸可调硼纳米反应器肿瘤的协同化学动力疗法。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-18 DOI: 10.1002/adhm.202402307

Lin Li, Qian Zhao, Zhiqing Chen, Zican Zhao, Baojie Du, Mixue Wang, Peirong Bai, Xiaozhe Wang, Xiaofeng Ren, Liping Li, Ruiping Zhang

{"title":"Size-Tunable Boron Nanoreactors for Boron Neutron Capture Synergistic Chemodynamic Therapy of Tumor.","authors":"Lin Li, Qian Zhao, Zhiqing Chen, Zican Zhao, Baojie Du, Mixue Wang, Peirong Bai, Xiaozhe Wang, Xiaofeng Ren, Liping Li, Ruiping Zhang","doi":"10.1002/adhm.202402307","DOIUrl":"https://doi.org/10.1002/adhm.202402307","url":null,"abstract":"Boron neutron capture therapy (BNCT) stands out as a noninvasive potential modality for invasive malignant tumors, with boron drugs playing a crucial role in its efficacy. Nevertheless, the development of boron drugs with biodegradability, as well as high permeability and retention effects, continues to present significant challenges. Here, we fabricate a size-tunable boron nanoreactor (TBNR) via assembling boron nitride quantum dots (BNQDs) and Fe3+ for tumor BNCT and chemodynamic (CDT) synergistic treatment. The obtained TBNR with an appropriate size exhibits superior tumor accumulation and retention. Upon stimulation by the tumor microenvironment (TME), the contained Fe3+ undergo redox reactions with glutathione (GSH) to produce Fe2+ Fenton reagents, which in turn activate CDT function and simultaneously induce TBNR depolymerization. Subsequently, the released ultrasmall BNQDs exhibit intra-deep penetration characteristic and are fully enriched at the tumor site. The in vivo experiments reveal that TBNR possesses excellent biocompatibility and superior synergistic anti-tumor ability post neutron irradiation, resulting in significant shrinkage of subcutaneous 4T1 tumors. Moreover, the TBNR-mediated BNCT has triggered an obvious immune response, which contributes to the long-term suppression of tumors after neutron irradiation. To conclude, this study provides a new approach for constructing more efficient versatile nanocarriers for BNCT-induced combination cancer therapies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402307"},"PeriodicalIF":10.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cordycepin-Loaded Dental Pulp Stem Cell-Derived Exosomes Promote Aged Bone Repair by Rejuvenating Senescent Mesenchymal Stem Cells and Endothelial Cells. 装载虫草素的牙髓干细胞衍生外泌体通过使衰老间充质干细胞和内皮细胞恢复活力促进老年骨修复

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-17 DOI: 10.1002/adhm.202402909

Yu Wang, Shanshan Jin, Yaru Guo, Lisha Zhu, Yilong Lu, Jing Li, Boon Chin Heng, Yan Liu, Xuliang Deng

{"title":"Cordycepin-Loaded Dental Pulp Stem Cell-Derived Exosomes Promote Aged Bone Repair by Rejuvenating Senescent Mesenchymal Stem Cells and Endothelial Cells.","authors":"Yu Wang, Shanshan Jin, Yaru Guo, Lisha Zhu, Yilong Lu, Jing Li, Boon Chin Heng, Yan Liu, Xuliang Deng","doi":"10.1002/adhm.202402909","DOIUrl":"https://doi.org/10.1002/adhm.202402909","url":null,"abstract":"Aging impairs bone marrow mesenchymal stem cell (BMSC) functions as well as associated angiogenesis which is critical for bone regeneration and repair. Hence, repairing bone defects in elderly patients poses a formidable challenge in regenerative medicine. Here, the engineered dental pulp stem cell-derived exosomes loaded with the natural derivative of adenosine Cordycepin (CY@D-exos) are fabricated by means of the intermittent ultrasonic shock, which dually rejuvenates both senescent BMSCs and endothelial cells and significantly improve bone regeneration and repair in aged animals. CY@D-exos can efficiently overcome the senescence of aged BMSCs and enhance their osteogenic differentiation by activating NRF2 signaling and maintaining heterochromatin stability. Importantly, CY@D-exos also potently overcomes the senescence of vascular endothelial cells and promotes angiogenesis. In vivo injectable gelatin methacryloyl (GelMA) hydrogels with sustained release of CY@D-exos can accelerate bone injury repair and promote new blood vessel formation in aged animals. Taken together, these results thus demonstrate that cordycepin-loaded dental pulp stem cell-derived exosomes display considerable potential to be developed as a next-generation therapeutic agent for promoting aged bone regeneration and repair.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402909"},"PeriodicalIF":10.0,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-Printed In Situ Growth of Bilayer MOF Hydrogels for Accelerated Osteochondral Defect Repair. 用于加速骨软骨缺损修复的双层 MOF 水凝胶三维打印原位生长。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-17 DOI: 10.1002/adhm.202403840

Kaiqi Qin, Xinyue Huang, Shengfeng Wang, Jiachen Liang, Zengjie Fan

{"title":"3D-Printed In Situ Growth of Bilayer MOF Hydrogels for Accelerated Osteochondral Defect Repair.","authors":"Kaiqi Qin, Xinyue Huang, Shengfeng Wang, Jiachen Liang, Zengjie Fan","doi":"10.1002/adhm.202403840","DOIUrl":"https://doi.org/10.1002/adhm.202403840","url":null,"abstract":"Repairing osteochondral (OC) defect presents a significant challenge due to the intricate structural requirements and the unpredictable differentiation pathways of bone marrow mesenchymal stem cells (BMSCs). To address this challenge, a novel biomimetic OC hydrogel scaffold is developed that features a structure of soft and hard components. This scaffold incorporates bilayer metal-organic frameworks (MOFs), specifically ZIF-67 in the upper layer and ZIF-8 in the lower layer, achieved through an in situ printing process. This configuration enables the spatial and temporal modulation of BMSC differentiation by controlling the release of Co2⁺ and Zn2⁺. The results demonstrate that the bilayer MOF hydrogels significantly outperform hydrogels that either lack MOFs or contain a single type of MOF in enhancing repair outcomes in rabbit models of knee OC defects. The improved regenerative efficacy is attributed to the distinct chondrogenic and osteogenic differentiation cues provided by the bilayer MOFs, effectively guiding BMSCs toward enhanced tissue regeneration. This customizable biomimetic OC hydrogel scaffold not only opens new avenues for innovative therapeutic strategies but also holds great promise for widespread clinical applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403840"},"PeriodicalIF":10.0,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0