Advanced Healthcare Materials最新文献_第2页

A Sustained H₂/Fluorouracil-Releasing Suppository for High-efficacy and Low-Toxicity Hydrogenochemotherapy of Colon Cancer.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-21 DOI: 10.1002/adhm.202404054

Danyang Chen, Zuan Wu, Chao Xia, Haiyan Yang, Wenjiang Ding, Qianjun He

{"title":"A Sustained H2/Fluorouracil-Releasing Suppository for High-efficacy and Low-Toxicity Hydrogenochemotherapy of Colon Cancer.","authors":"Danyang Chen, Zuan Wu, Chao Xia, Haiyan Yang, Wenjiang Ding, Qianjun He","doi":"10.1002/adhm.202404054","DOIUrl":"https://doi.org/10.1002/adhm.202404054","url":null,"abstract":"To attenuate the intestinal toxicity of chemotherapeutic drugs from rectal suppositories and enhance their chemotherapeutic outcome is greatly significant, but maintains a challenge. In this work, a new strategy of local synergistic hydrogenochemotherapy is proposed to attenuate side effects and enhance therapeutic efficacy based on the anti-cancer selectivity and normal cells-protecting effect of H2, and construct a novel anti-cancer formulation of rectal suppository (5-FU/CSN@FAG) by fatty acid glycerides (FAG) encapsulating 5-fluorouracil (5-FU, a first-line drug for colorectal cancer treatment) and cerium silicide nanoparticles (CSN) with a sustained hydrolytic H2 release behavior which is synchronous with 5-FU release. The 3-week treatment with the suppository once a day can not only completely eradicate colon tumors without tumor recurrence after suppository administration withdrawal, but also efficiently protect the intestinal tract from chemotherapeutic damage. Mechanistically, H2 generated by CSN reduces the toxicity of 5-FU to normal cells in the intestinal tract by scavenging over-expressed reactive oxygen species and correcting energy metabolism, and also assists 5-FU to promote the apoptosis of colon tumor cells by inhibiting their respiration through a CO signaling pathway. High biosafety and therapeutic validity endow the developed suppository with a high potential for clinical translation.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404054"},"PeriodicalIF":10.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosynthesis-Inspired NIR-Triggered Fe₃O₄@MoS₂ Core-Shell Nanozyme for Promoting MRSA-Infected Diabetic Wound Healing.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-20 DOI: 10.1002/adhm.202404525

Jiamu Xiao, Xuping An, Fei Tang, Xu Dai, Song Zhang, Xiaolong Zhu, Jian Shen, Jiang Yuan, Donglin Gan, Mingqian Wang

{"title":"Photosynthesis-Inspired NIR-Triggered Fe₃O₄@MoS₂ Core-Shell Nanozyme for Promoting MRSA-Infected Diabetic Wound Healing.","authors":"Jiamu Xiao, Xuping An, Fei Tang, Xu Dai, Song Zhang, Xiaolong Zhu, Jian Shen, Jiang Yuan, Donglin Gan, Mingqian Wang","doi":"10.1002/adhm.202404525","DOIUrl":"https://doi.org/10.1002/adhm.202404525","url":null,"abstract":"Bacterial infections can lead to severe medical complications, including major medical incidents and even death, posing a significant challenge in clinical trauma repair. Consequently, the development of new, efficient, and non-resistant antimicrobial agents has become a priority for medical practitioners. In this study, a stepwise hydrothermal reaction strategy is utilized to prepare Fe3O4@MoS2 core-shell nanoparticles (NPs) with photosynthesis-like activity for the treatment of bacterial infections. The Fe3O4@MoS2 NPs continuously catalyze the production of reactive oxygen species (ROS) from hydrogen peroxide through photosynthesis-like reactions and convert light energy into heat with a photothermal efficiency of 30.30%. In addition, the photosynthetically generated ROS, combined with the iron-induced cell death mechanism of the Fe3O4@MoS2 NPs, confer them with exceptional and broad-spectrum antibacterial properties, achieving antimicrobial activities of up to 98.62% for Staphylococcus aureus, 99.22% for Escherichia coli, and 98.55% for methicillin-resistant Staphylococcus aureus. The composite exhibits good cell safety and hemocompatibility. Finally, a full-thickness diabetic wound model validates the significant pro-healing properties of Fe3O4@MoS2 in chronic diabetic wounds. Overall, the design of photosynthesis-inspired Fe3O4@MoS2 presents new perspectives for developing efficient photothermal nano-enzymatic compounds, offering a promising solution to the challenges of antimicrobial drug resistance and antibiotic misuse.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404525"},"PeriodicalIF":10.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macro/Microgel-Encapsulated, Biofilm-Armored Living Probiotic Platform for Regenerating Bacteria-Infected Diabetic Wounds.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-20 DOI: 10.1002/adhm.202403476

Huilong Xin, Zhe Cai, Jiahui Hao, Jing An, Yi Li, Min Wen, Zhaojun Jia

{"title":"Macro/Microgel-Encapsulated, Biofilm-Armored Living Probiotic Platform for Regenerating Bacteria-Infected Diabetic Wounds.","authors":"Huilong Xin, Zhe Cai, Jiahui Hao, Jing An, Yi Li, Min Wen, Zhaojun Jia","doi":"10.1002/adhm.202403476","DOIUrl":"https://doi.org/10.1002/adhm.202403476","url":null,"abstract":"Infectious diabetic wounds pose an arduous threat to contemporary healthcare. The combination of refractory biofilms, persistent inflammation, and retarded angiogenesis can procure non-unions and life-threatening complications, calling for advanced therapeutics potent to orchestrate anti-infective effectiveness, benign biocompatibility, pro-reparative immunomodulation, and angiogenic regeneration. Herein, embracing the emergent \"living bacterial therapy\" paradigm, a designer probiotic-in-hydrogel wound dressing platform is demonstrated. The platform is constructed employing a \"macrogel/microgel/biofilm\" hierarchical encapsulation strategy, with Lactobacillus casei as the model probiotic. Alginate gels, in both macro and micro forms, along with self-produced probiotic biofilms, served as encapsulating agents. Specifically, live probiotics are enclosed within alginate microspheres, embedded into an alginate bulk matrix, and cultivated to facilitate biofilm self-encasing. This multiscale confinement protected the probiotics and averted their inadvertent escape, while enabling sustained secretion, proper reservation, and localized delivery of therapeutically active probiotic metabolites, such as lactic acid. The resulting biosystem, as validated in vitro/ovo/vivo, elicited well-balanced antibacterial activities and biological compatibility, alongside prominent pro-healing, vasculogenic and anti-inflammatory potencies, thus accelerating the regeneration of infected full-thickness excisional wounds in diabetic mice. Such multiple encapsulation-engineered \"all-in-one\" probiotic delivery tactic may shed new light on the safe and efficient adoption of live bacteria for treating chronic infectious diseases.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403476"},"PeriodicalIF":10.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acid-Triggered Cascaded Responsive Supramolecular Peptide Alleviates Myocardial Ischemia‒Reperfusion Injury by Restoring Redox Homeostasis and Protecting Mitochondrial Function.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-20 DOI: 10.1002/adhm.202404319

Xu Liao, Min Tang, Jiejing Li, Runze Guo, Chongbin Zhong, Xiangzhou Chen, Xuwei Zhang, Hongwei Mo, Dongdong Que, Wenjie Yu, Xudong Song, Hekai Li, Yanbin Cai, Pingzhen Yang

{"title":"Acid-Triggered Cascaded Responsive Supramolecular Peptide Alleviates Myocardial Ischemia‒Reperfusion Injury by Restoring Redox Homeostasis and Protecting Mitochondrial Function.","authors":"Xu Liao, Min Tang, Jiejing Li, Runze Guo, Chongbin Zhong, Xiangzhou Chen, Xuwei Zhang, Hongwei Mo, Dongdong Que, Wenjie Yu, Xudong Song, Hekai Li, Yanbin Cai, Pingzhen Yang","doi":"10.1002/adhm.202404319","DOIUrl":"https://doi.org/10.1002/adhm.202404319","url":null,"abstract":"Redox imbalance, including excessive production of reactive oxygen species (ROS) caused by mitochondrial dysfunction and insufficient endogenous antioxidant capacity, is the primary cause of myocardial ischemia‒reperfusion (I/R) injury. In the exploration of reducing myocardial I/R injury, it is found that protecting myocardial mitochondrial function after reperfusion not only reduces ROS bursts but also inhibits cell apoptosis triggered by the release of cytochrome c. Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2) is considered a potential therapeutic target for treating myocardial I/R injury by enhancing the cellular antioxidant capacity through the induction of endogenous antioxidant enzymes. In this study, a peptide‒drug conjugate OI-FFG-ss-SS31(ISP) is developed by integrating the Nrf2 activator 4-octyl itaconate (OI) and the mitochondria-targeting protective peptide elamipretide (SS31), and its therapeutic potential for myocardial I/R injury is explored. The results showed that ISP could self-assemble into nanofibers in response to the acidic microenvironment and bind to Keap-1 with high affinity, thereby activating Nrf2 and enhancing antioxidant capacity. Simultaneously, the release of SS31 could improve mitochondrial function and reduce ROS, ultimately providing a restoration of redox homeostasis to effectively alleviate myocardial I/R injury. This study presents a promising acid-triggered peptide-drug conjugate for treating myocardial I/R injury.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404319"},"PeriodicalIF":10.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucocorticoids Alter Bone Microvascular Barrier via MAPK/Connexin43 Mechanisms.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-20 DOI: 10.1002/adhm.202404302

Eun-Jin Lee, Peter Lialios, Micaila Curtis, James Williams, Yoontae Kim, Paul Salipante, Steven Hudson, Mandy B Esch, Moshe Levi, Joanna Kitlinska, Stella Alimperti

{"title":"Glucocorticoids Alter Bone Microvascular Barrier via MAPK/Connexin43 Mechanisms.","authors":"Eun-Jin Lee, Peter Lialios, Micaila Curtis, James Williams, Yoontae Kim, Paul Salipante, Steven Hudson, Mandy B Esch, Moshe Levi, Joanna Kitlinska, Stella Alimperti","doi":"10.1002/adhm.202404302","DOIUrl":"https://doi.org/10.1002/adhm.202404302","url":null,"abstract":"Glucocorticoids (GCs) are standard-of-care treatments for inflammatory and immune disorders, and their long-term use increases the risk of osteoporosis. Although GCs decrease bone functionality, their role in bone microvasculature is incompletely understood. Herein, the study investigates the mechanisms of bone microvascular barrier function via osteoblast-endothelial interactions in response to GCs. The animal data shows that prednisolone (Psl) downregulated the osteoblast function and microvessel number and size. To investigate the role of GCs in bone endothelial barrier function further, a bicellular microfluidic in vitro system is developed and utilized, which consists of three-dimensional (3D) perfusable microvascular structures embedded in collagen I/osteoblast matrix. Interestingly, it is demonstrated that GCs significantly inhibit osteogenesis and microvascular barrier function by interfering with endothelial-osteoblast interactions. This effect is triggered by MAPK-induced phosphorylation of connexin43 (Cx43) at Ser282. Collectively, this study sheds light on microvascular function in bone disorders, as osteoporosis, and permits to capture dynamic changes in endothelial-bone interactions under GCs by dissecting the MAPK/Cx43 mechanism and proposing this as a potential target for bone diseases.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404302"},"PeriodicalIF":10.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive Silk Cryogel Dressing with Multiple Physical Cues to Control Cell Migration and Wound Regeneration.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-20 DOI: 10.1002/adhm.202404304

Yaqian Chen, Xiaoyi Zhang, Mengting Wang, Yu Liang, Zhaozhu Zheng, Meng Liu, Qiang Lu

{"title":"Bioactive Silk Cryogel Dressing with Multiple Physical Cues to Control Cell Migration and Wound Regeneration.","authors":"Yaqian Chen, Xiaoyi Zhang, Mengting Wang, Yu Liang, Zhaozhu Zheng, Meng Liu, Qiang Lu","doi":"10.1002/adhm.202404304","DOIUrl":"https://doi.org/10.1002/adhm.202404304","url":null,"abstract":"Introducing multiple physical cues to control cell behaviors effectively is considered as a promising strategy in developing bioactive wound dressings. Silk nanofiber-based cryogels are developed to favor angiogenesis and tissue regeneration through tuning hydrated state, microporous structure, and mechanical property, but remained a challenge to endow with more physical cues. Here, β-sheet rich silk nanofibers are used to develop cryogels with nanopore structure. Through optimizing crosslinking time and exposing the reactive group inside the nanofibers, the crosslinking reaction is improved to induce stable cryogel formation. Besides the hydrated state and macroporous structure, the nanopore structure formed on the macroporous walls, providing hierarchical microstructures to improve cell migration. Both in vitro and in vivo results reveal quicker cell migration inside the cryogels, which then accelerates angiogenesis and wound healing. The mechanical properties can further regulate to match with skin regeneration. The wound healing study in vivo reveals lower inflammatory factor secretion in the wounds treated with softer cryogels with nanopores, which then resulted in the best angiogenesis and wound healing with less scar. Therefore, the porous cryogels with multiple physical cues can be fabricated with silk nanofibers to control cell behaviors and tissue regeneration, providing a promising approach for designing bioactive wound dressings.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404304"},"PeriodicalIF":10.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid Crystalline Networks Hamper the Malignancy of Cancer Cells.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-19 DOI: 10.1002/adhm.202403607

Daniele Martella, Ignazia Tusa, Alessandro Tubita, Alessia Negri, Marco Turriani, Marta Rojas-Rodríguez, Martina Salzano de Luna, Alessio Menconi, Camilla Parmeggiani, Elisabetta Rovida

{"title":"Liquid Crystalline Networks Hamper the Malignancy of Cancer Cells.","authors":"Daniele Martella, Ignazia Tusa, Alessandro Tubita, Alessia Negri, Marco Turriani, Marta Rojas-Rodríguez, Martina Salzano de Luna, Alessio Menconi, Camilla Parmeggiani, Elisabetta Rovida","doi":"10.1002/adhm.202403607","DOIUrl":"https://doi.org/10.1002/adhm.202403607","url":null,"abstract":"Mimicking compositions and structures of extracellular matrix is widely studied to create in vitro tumor models, to deepen the understanding of the pathogenesis of the different types of cancer, and to identify new therapies. On the other hand, the use of synthetic materials to modulate cancer cell biology and, possibly, to reduce the malignancy of cancer cells through their exploitation is far less explored. Here, the study evaluates the effects of Liquid Crystalline Networks (LCNs) based scaffolds on the growth of A375 metastatic melanoma cells. Interestingly, cells grown on such materials show reduced cell proliferation and colony-forming capacity with respect to those cultivated on standard plates. These effects are associated with a higher percentage of senescent cells and a shift to a more epithelial phenotype, pointing to the occurrence of a mesenchymal to epithelial transition. All these biological outcomes are affected by the amount of crosslinker in the material and have been induced only thanks to the interactions with the polymeric substrate without the need of further chemical (e.g., specific growth factor) or physical (e.g., irradiation) stimuli, opening to the possible development of anti-cancer coatings.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403607"},"PeriodicalIF":10.0,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acceptor Elongation Boosted Intersystem Crossing Affords Efficient NIR Type-I and AIE-Active Photosensitizers for Targeting Ferroptosis-Based Cancer Therapy.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-19 DOI: 10.1002/adhm.202404505

Yilin Zhu, Rongyuan Zhang, Xu-Min Cai, Liping Zhang, Bo Wu, Haozhe Tan, Kun Zhou, Haoran Wang, Yong Liu, Yumei Luo, Ryan T K Kwok, Jacky W Y Lam, Zheng Zhao, Cuiping Yao, Ben Zhong Tang

{"title":"Acceptor Elongation Boosted Intersystem Crossing Affords Efficient NIR Type-I and AIE-Active Photosensitizers for Targeting Ferroptosis-Based Cancer Therapy.","authors":"Yilin Zhu, Rongyuan Zhang, Xu-Min Cai, Liping Zhang, Bo Wu, Haozhe Tan, Kun Zhou, Haoran Wang, Yong Liu, Yumei Luo, Ryan T K Kwok, Jacky W Y Lam, Zheng Zhao, Cuiping Yao, Ben Zhong Tang","doi":"10.1002/adhm.202404505","DOIUrl":"https://doi.org/10.1002/adhm.202404505","url":null,"abstract":"Photosensitizers (PSs) featuring type I reactive oxygen species (ROS) generation and aggregation-induced emission (AIE) activity offer a promising solution to achieve non-invasive and precise theranostics. However, the reported AIE luminogens (AIEgens) with both AIE characteristic and strong type-I ROS generation are still scarce and the structure-property relationship is still unclear. Herein, an innovative acceptor elongation boosted intersystem crossing (AEBIC) design strategy has been proposed to endow the AIEgen strong type-I ROS producibility. The results indicate that the obtained AIEgen exhibit type-I ROS and aggregation-enhanced ROS efficacy, which has been verified by both experimental and theoretical results. Mechanistic study reveal that the acceptor elongation has promoted a dual-channel intersystem crossing pathway to enhance the intersystem crossing (ISC) process due to the differences in triplet configurations, which can be further amplified by aggregation. The afforded type-I AIE-PS show lipid droplet-anchored characteristic and can induce the ferroptosis through destroying the cellular redox homeostasis and increasing lethal levels of lipid peroxidation. Finally, targeting ferroptosis-based cancer therapy can be realized with excellent anti-tumor effect.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404505"},"PeriodicalIF":10.0,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Modified Polydopamine Nanoparticle Loaded with Melatonin for Synergistic ROS Scavenging and Anti-Inflammatory Effects in the Treatment of Dry Eye Disease.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-19 DOI: 10.1002/adhm.202404372

Xue Yang, Bowen Wang, Hao Zeng, Lihong Liang, Runze Zhang, Wenrui Deng, Xuan Zhao, Jin Yuan

{"title":"A Modified Polydopamine Nanoparticle Loaded with Melatonin for Synergistic ROS Scavenging and Anti-Inflammatory Effects in the Treatment of Dry Eye Disease.","authors":"Xue Yang, Bowen Wang, Hao Zeng, Lihong Liang, Runze Zhang, Wenrui Deng, Xuan Zhao, Jin Yuan","doi":"10.1002/adhm.202404372","DOIUrl":"https://doi.org/10.1002/adhm.202404372","url":null,"abstract":"Dry eye disease (DED) is a multifaceted ocular surface disorder that significantly impacts patients' daily lives and imposes a substantial economic burden on society. Oxidative stress, induced by the overproduction of reactive oxygen species (ROS), is a critical factor perpetuating the inflammatory cycle in DED. Effectively scavenging ROS is essential to impede the progression of DED. In this study, boronophenylalanine- containing polydopamine (PDA-PBA) nanoparticles are developed loaded with melatonin (MT), which are blended with poly(vinyl alcohol) (PVA) to create eye drops PVA/ PDA-PBA@MT (PPP@MT). In vitro and in vivo studies demonstrate that PPP@MT exhibits dual functionalities in reducing ROS production and downregulating inflammatory pathways, thereby preserving mitochondrial integrity and further inhibiting programmed cell death. Following PPP@MT treatment, tear secretion, corneal structure, and the number of goblet cells are markedly restored in a mouse model of dry eye, indicating the therapeutic efficacy of this agent. Collectively, PPP@MT, characterized by minimal side effects and favorable bioavailability, offers promising therapeutic insights for the management of DED and other ROS-mediated disorders.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404372"},"PeriodicalIF":10.0,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dexamethasone Delivery via Amphiphilic, Low-swelling Hydrogels Treats Postoperative Inflammation in Cervical Spine Applications.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-19 DOI: 10.1002/adhm.202404292

Stephen W Linderman, Graham F Barber, S'Dravious A DeVeaux, Edward A Botchwey, Daniel Refai, Adam M Klein, Andrés J García

{"title":"Dexamethasone Delivery via Amphiphilic, Low-swelling Hydrogels Treats Postoperative Inflammation in Cervical Spine Applications.","authors":"Stephen W Linderman, Graham F Barber, S'Dravious A DeVeaux, Edward A Botchwey, Daniel Refai, Adam M Klein, Andrés J García","doi":"10.1002/adhm.202404292","DOIUrl":"https://doi.org/10.1002/adhm.202404292","url":null,"abstract":"Anterior cervical spine surgeries are often complicated by difficulty swallowing due to local postoperative swelling, pain, scarring, and tissue dysfunction. These postoperative events lead to systemic steroid and narcotic use. Local, sustained drug delivery may address these problems, but current materials are unsafe for tight surgical spaces due to high biomaterial swelling, especially upon degradation. To address these shortcomings, a low-swelling, amphiphilic hydrogel system termed DexaPatch is developed containing dexamethasone-poly(lactic-co-glycolic acid) (PLGA) microparticles for sustained release upon local implantation in the surgical site. The bulk amphiphilic hydrogel, comprised of 4-arm poly(ethylene glycol) (PEG)-maleimide macromer cross-linked with triblock dithiolated PEG-poly(propylene glycol)-PEG (poloxamer a.k.a. Pluronic), achieves consistent and tunable mechanical and low-swelling properties. Dexamethasone is released in a burst, followed by a sustained release over 40 days, similar to the release from microparticles alone. The DexaPatch system is lyophilized for shelf stability and surgical handling properties, sterilized, and briefly rehydrated in the operating room prior to surgical implantation in a rabbit model of anterior spinal surgery. DexaPatch results in significantly reduced prevertebral edema radiographically and decreased fibrosis in prevertebral muscles compared to sham surgery. This implantable biomaterial platform reduces local postoperative inflammation with potential surgical applications throughout the body.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404292"},"PeriodicalIF":10.0,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0