Advanced Healthcare Materials最新文献_第2页

Geometrically Tunable Scaffold-Free Muscle Bioconstructs for Treating Volumetric Muscle Loss. 几何可调无支架肌肉生物结构治疗体积性肌肉损失。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202501887

Bugra Ayan, Gaoxian Chen, Ishita Jain, Sha Chen, Gladys Chiang, Caroline Hu, Renato Reyes, Beu P Oropeza, Ngan F Huang

{"title":"Geometrically Tunable Scaffold-Free Muscle Bioconstructs for Treating Volumetric Muscle Loss.","authors":"Bugra Ayan, Gaoxian Chen, Ishita Jain, Sha Chen, Gladys Chiang, Caroline Hu, Renato Reyes, Beu P Oropeza, Ngan F Huang","doi":"10.1002/adhm.202501887","DOIUrl":"https://doi.org/10.1002/adhm.202501887","url":null,"abstract":"Traumatic muscle injuries associated with volumetric muscle loss (VML) are characterized by muscle loss beyond intrinsic regeneration capacity, leading to permanent functional impairment. Experimental therapies to augment muscle regeneration, such as cell injection, are limited by low cell transplantation capacity, whereas conventional engineered muscle tissue transplants lack geometric customization to conform to the shape of the muscle defect. Here, a facile approach to engineer scaffold-free high-density muscle tissues in customizable geometric shapes and sizes with high cell viability and integration potential is developed. Using a facile mold-based approach to engineer scaffold-free modular units, transcriptional profiling is performed to uncover the role of pre-formed cell-cell interactions within scaffold-free muscle bioconstructs on myogenesis, an the efficacy of muscle bioconstructs in a mouse model of VML is then evaluated. RNA sequencing revealed that pre-formed cell-cell interactions supported myogenic pathways related to muscle contraction and myofibril assembly, unlike dissociated monodisperse cells. This work further demonstrates the therapeutic efficacy of 3D rectangular solid-shaped scaffold-free transplants in improving muscle function and vascular regeneration. Finally, toward clinical translation, the feasibility of this technology to integrate with medical imaging and artificial intelligence-driven customized bioconstruct design and assembly for intraoperative use is illustrated.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e01887"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling. 分区肾体外疾病建模三维合成平台的开发

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503287

Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte

{"title":"Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling.","authors":"Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte","doi":"10.1002/adhm.202503287","DOIUrl":"https://doi.org/10.1002/adhm.202503287","url":null,"abstract":"3D in vitro tissue and disease models have emerged as an important tool for diagnostic and therapeutic screenings, as they offer a closer approximation toward native environments than traditional 2D cell culture. Kidney disease modeling in particular has progressed to using induced pluripotent stem cells (iPSCs) and microfluidic platforms to replicate the complex microenvironment of the kidney. However, current models lack mature tissue development, scalability, tunability, and spatial organization. In this study, a fully synthetic, 3D kidney disease platform that addresses these challenges is presented. This model comprises a compartmentalized poly (ethylene glycol) (PEG)-based hydrogel matrix with anisotropic PEG-based microgels. This multiphasic hydrogel system provides control over spatially organizing a triple-co-culture of key renal cell types: tubule-epithelial cells (CD10+), endothelial cells (CD31+), and fibroblasts (PDGFRβ+). Structural control and compartmentalization are enabled through enzymatically degradable rod microgels produced using microfluidics, allowing for a modular system. This study characterizes the synthetic models and analyzes the functionality of the system by examining cell-material interactions. The use of this system as a promising disease model is demonstrated through the addition of TGFβ, inducing fibrosis. This work highlights a novel approach to building a fully synthetic, scalable, modular kidney model with a tunable microenvironment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03287"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pathophysiology-Informed, Negentropy-Oriented Strategy for Nanomedicine in MASLD. 基于病理生理学、负熵导向的MASLD纳米医学策略。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202504298

Rui Mao, Meng Yu, Xiu-Ping Guo, Xiao-Lian Tian, Meng-Yu Zhao, Quan-Yong Yu, Gang Ren, Ming-Yu Pan, Ru Bai, Li-Ping Liu, Gui-Ling Li, Jian-Dong Jiang, Lu-Lu Wang

{"title":"A Pathophysiology-Informed, Negentropy-Oriented Strategy for Nanomedicine in MASLD.","authors":"Rui Mao, Meng Yu, Xiu-Ping Guo, Xiao-Lian Tian, Meng-Yu Zhao, Quan-Yong Yu, Gang Ren, Ming-Yu Pan, Ru Bai, Li-Ping Liu, Gui-Ling Li, Jian-Dong Jiang, Lu-Lu Wang","doi":"10.1002/adhm.202504298","DOIUrl":"https://doi.org/10.1002/adhm.202504298","url":null,"abstract":"Metabolic dysfunction-associated steatotic liver disease (MASLD) is a multifactorial chronic liver disorder driven by an ensemble of interrelated pathological processes, including insulin resistance, lipid accumulation, oxidative stress, immune dysregulation, gut microbiota imbalance, and hepatocyte injury-induced cell death. These overlapping mechanisms pose significant challenges for effective treatment, as conventional single-target therapies often fail to address the systemic complexity of the disease. Recent advances in functional nanomedicine have introduced promising avenues for MASLD intervention by enabling the development of nanoplatforms specifically engineered to interact with disease-specific pathophysiological features. These systems incorporate stimuli-responsive drug release, targeted hepatic accumulation, and intrinsic therapeutic activity, allowing for simultaneous modulation of multiple pathological pathways. This review presents a pathophysiology-informed framework for nanomedicine design in MASLD therapy. How diverse platforms are strategically tailored to regulate reactive oxygen species (ROS) production, modulate immune imbalance, restore insulin signaling, inhibit ferroptosis, and rebalance gut microbial dysbiosis is examined. Moreover, emerging approaches such as carrier-free, self-assembling systems and multifunctional yet intentionally minimalist architectures that enhance translational potential are highlighted. Together, these strategies exemplify a shift toward mechanism-driven, entropy-informed nanotherapeutics, wherein negentropy-oriented and leading-axis design principles offer a promising roadmap for restoring metabolic homeostasis in complex disease contexts such as MASLD.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e04298"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staged Low-Frequency Ultrasound Synergized with Composite Hydrogel to Achieve "Rapid Antimicrobial-Ordered Regeneration" of Infected Wounds. 阶段低频超声与复合水凝胶协同实现感染伤口“快速抗菌有序再生”。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503471

Yixin Li, Guangrong Zheng, Ying Lu, Jiaqi Liu, Fang Qin, Qingyang Zhou, Liangcan He, Xiang Mao

{"title":"Staged Low-Frequency Ultrasound Synergized with Composite Hydrogel to Achieve \"Rapid Antimicrobial-Ordered Regeneration\" of Infected Wounds.","authors":"Yixin Li, Guangrong Zheng, Ying Lu, Jiaqi Liu, Fang Qin, Qingyang Zhou, Liangcan He, Xiang Mao","doi":"10.1002/adhm.202503471","DOIUrl":"https://doi.org/10.1002/adhm.202503471","url":null,"abstract":"Bacterial-infectious wounds present a significant threat to global health due to an altered microenvironment that promotes drug-resistant bacterial persistence. Given the limitations of traditional dressings, static drug release mechanisms, and singular antibacterial action, along with the issue of drug resistance exacerbated by the overuse of antibiotics, a dual dynamic cross-linked network composite hydrogel (CSGA-Cip) is developed to overcome these challenges. The hydrogel forms a dynamic adaptive network through Schiff base bonds and Ag─S coordination bonds, endowing it with injectable and self-healing properties. Notably, a staged low-frequency ultrasound intervention strategy is employed to achieve a cascade treatment of antibacterial ordered regeneration. During acute infection (0-3 days), continuous low-frequency ultrasound facilitates the rapid release of silver ions (Ag⁺) and ciprofloxacin (Cip), synergizing with glutathione (GSH) to establish multiple antibacterial barriers. In the repair stage (3-9 days), pulsed low-frequency ultrasound activated cell migration and angiogenesis signaling pathways to guide tissue regeneration. Animal studies have confirmed that this strategy significantly enhances infectious wound healing, promotes orderly collagen deposition, and stimulates angiogenesis, thereby providing an innovative paradigm for the treatment of infectious wounds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03471"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Computational Journey Toward an Optimal Design for Metamaterial Epicardial Passive Sleeves. 超材料心外膜被动套筒优化设计的计算之旅。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202501369

Vahid Naeini, Emilio A Mendiola, Ahmad Rafsanjani, Fergal B Coulter, Qian Xiang, Jianyi Zhang, Peter Vanderslice, Vahid Serpooshan, Reza Avazmohammadi

{"title":"A Computational Journey Toward an Optimal Design for Metamaterial Epicardial Passive Sleeves.","authors":"Vahid Naeini, Emilio A Mendiola, Ahmad Rafsanjani, Fergal B Coulter, Qian Xiang, Jianyi Zhang, Peter Vanderslice, Vahid Serpooshan, Reza Avazmohammadi","doi":"10.1002/adhm.202501369","DOIUrl":"https://doi.org/10.1002/adhm.202501369","url":null,"abstract":"Heart failure (HF) following myocardial infarction (MI) is a major clinical challenge with severe complications. Epicardial sleeves and patches are increasingly investigated to improve heart function post-MI, yet their passive mechanical effects remain underexplored. This has resulted in limited insight into how sleeves mechanically interact with the infarct and remote myocardium. This study used 3-D in-silico cardiac models to examine how sleeve shape, material properties, and architecture affect global and regional mechanics. A high-fidelity biventricular model is used to investigate how a continuum cardiac sleeve alters function. Designs that improve regional mechanics successfully limited pathological bulging, modulated fiber strains, and influenced torsional behavior without over-constraining remote tissue, whereas overly restrictive and stiff sleeves penalized healthy myocardium and reduced the intended relief of infarct bulging. These findings highlight the importance of considering regional biomechanical markers when developing sleeve designs. Building on these continuum sleeve insights, a spheroidal left ventricle model demonstrated the proof-of-concept advantage of an \"auxetic\" metamaterial sleeve, engineered with a negative Poisson ratio. This programmed architecture provided region-specific benefits beyond those of conventional continuum sleeves. Ultimately, this work contributes to an improved understanding of passive sleeve-heart interactions and improves the targeted biomechanical support therapies following MI.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e01369"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liver Organoid and Liver-On-A-Chip Platforms for Modeling Alcoholic Liver Disease: A Comparative Review. 肝脏类器官和肝脏芯片平台模拟酒精性肝病：比较回顾

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503273

Qiannan Chen, Chengpan Li, Weiping Ding, Derun Kong

{"title":"Liver Organoid and Liver-On-A-Chip Platforms for Modeling Alcoholic Liver Disease: A Comparative Review.","authors":"Qiannan Chen, Chengpan Li, Weiping Ding, Derun Kong","doi":"10.1002/adhm.202503273","DOIUrl":"https://doi.org/10.1002/adhm.202503273","url":null,"abstract":"Alcoholic liver disease (ALD) encompasses a spectrum of progressive liver injuries caused by chronic alcohol consumption, including steatosis, hepatitis, fibrosis, and cirrhosis. The development of physiologically relevant preclinical models remains critical for elucidating the pathogenesis of ALD and evaluating therapeutic strategies. Recent advances in liver organoid and liver-on-a-chip (LOC) technologies offer complementary platforms for modeling distinct aspects of ALD. Organoids recapitulate liver tissue architecture with multicellular composition, enabling the study of chronic pathological processes such as lipid accumulation and early fibrogenesis. Conversely, LOC systems replicate dynamic microenvironments such as fluid flow and oxygen gradients, enabling studies of inflammation and vascular injury. In this review, the first comprehensive comparison of liver organoids and LOC systems specifically in the context of ALD is provided. Emerging liver organoid-on-a-chip (OoC) strategies and their potential to model the full spectrum of ALD pathology are also discussed. Finally, key technical challenges are identified, and future directions are proposed targeting patient-specific, multiorgan, and high-throughput platforms to advance ALD-related research and precision medicine.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03273"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Injectable Hydrogel Co-Delivering Propranolol and Imiquimod to Synergistically Enhance Immunogenic Cell Death after Melanoma Resection. 可注射水凝胶联合递送心得安和咪喹莫特协同提高黑色素瘤切除术后免疫原性细胞死亡。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503019

Yunfeng Tang, Xiangtian Deng, Min Yi, Dong Wang, Cheng Zheng, Shichang Zhao, Renliang Zhao

{"title":"An Injectable Hydrogel Co-Delivering Propranolol and Imiquimod to Synergistically Enhance Immunogenic Cell Death after Melanoma Resection.","authors":"Yunfeng Tang, Xiangtian Deng, Min Yi, Dong Wang, Cheng Zheng, Shichang Zhao, Renliang Zhao","doi":"10.1002/adhm.202503019","DOIUrl":"https://doi.org/10.1002/adhm.202503019","url":null,"abstract":"Melanoma remains one of the most aggressive skin cancers with high recurrence rates following surgical resection. To overcome the challenges of residual tumor cells and postoperative immune resistance, an injectable hydrogel is developed that co-delivers propranolol (Pro) and imiquimod (IMQ). The rationale is to achieve a synergistic therapeutic effect by enhancing immunogenic cell death (ICD) and strengthening antitumor immunity. The hydrogel is synthesized via boronate ester crosslinking between HA-EGCG and CMCS-PBA and demonstrates excellent injectability, self-healing, biodegradability, and tissue adhesion. In vitro, Pro potentiated IMQ-induced reactive oxygen species (ROS) production, enhancing ICD markers such as calreticulin exposure (CRT), high mobility group box 1 (HMGB1), and adenosine triphosphate (ATP) release, thereby promoting dendritic cell maturation and T cell activation. In vivo, Pro/IMQ@ Hydrogel (PIH) effectively inhibited tumor recurrence in an incomplete melanoma resection model, significantly reducing tumor volume and inducing apoptosis without systemic toxicity. Immunohistochemical analyses revealed increased CD8⁺ and CD4⁺ T cell infiltration, elevated inflammatory cytokine levels, and enhanced memory T cell responses. This work highlights the potential of PIH as a potent localized chemo-immunotherapy platform for melanoma by converting \"cold\" tumors into \"hot\" immunogenic lesions.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03019"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable and pH-Activated Self-Feedback Hydrogel Encapsulating Stem Cells for Augmented Acute Myocardial Infarction Therapy. 可注射和ph活化的自反馈水凝胶包封干细胞用于增强急性心肌梗死治疗。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503324

Yan Kong, Ling Wang, Fei Duan, Chao Huang, Zhiwen Qiu, Rui-Juan Ji, Liang Qiao, Yang Zhou, Ting Cai, Yu-Quan Li, Wei Li, Xiang-Qun Yang

{"title":"Injectable and pH-Activated Self-Feedback Hydrogel Encapsulating Stem Cells for Augmented Acute Myocardial Infarction Therapy.","authors":"Yan Kong, Ling Wang, Fei Duan, Chao Huang, Zhiwen Qiu, Rui-Juan Ji, Liang Qiao, Yang Zhou, Ting Cai, Yu-Quan Li, Wei Li, Xiang-Qun Yang","doi":"10.1002/adhm.202503324","DOIUrl":"https://doi.org/10.1002/adhm.202503324","url":null,"abstract":"Hydrogels encapsulating stem cells represent a promising strategy for enhancing cardiac function following acute myocardial infarction (AMI). However, hostile post-infarct microenvironments, characterized by oxidative stress and ischemia, significantly reduce stem cell retention and survival rates. Herein, an intramyocardially injectable and pH-responsive (2' Z, 3' E)-6-Bromoindirubin-3'-oxime (BIO)-N-adipose-derived stem cells (ADSCs)-Matrigel system is developed for treating AMI. Encapsulated ADSCs exhibited a high retention rate in myocardial tissue. Furthermore, BIO-N exhibited a self-feedback function, enabling it to modulate the release of BIO in response to pH changes in the microenvironment during the progression of AMI. This system effectively protected stem cells and cardiomyocytes from ROS-induced injury while enhancing the therapeutic efficacy of ADSCs by improving their paracrine function. Subsequently, it is demonstrated that the BIO-N-ADSCs-Matrigel system significantly reduced infarction size, mitigated fibrosis, and enhanced local angiogenesis in a rat model of AMI. The self-feedback functional BIO-N-ADSCs-Matrigel system can effectively mitigate local oxidative stress, enhance the survival rate and therapeutic efficacy of stem cells, and improve the viability of cardiomyocytes for AMI treatment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03324"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Bioconjugate Materials: Synthesis, Characterization and Medical Applications. 新型生物偶联材料：合成、表征及医学应用。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202500303

Ellie Martin, Sean P Doidge, Eiman Aleem, Suela Kellici, Steven Dunn, Claire Atkinson, Philip D Howes

{"title":"Novel Bioconjugate Materials: Synthesis, Characterization and Medical Applications.","authors":"Ellie Martin, Sean P Doidge, Eiman Aleem, Suela Kellici, Steven Dunn, Claire Atkinson, Philip D Howes","doi":"10.1002/adhm.202500303","DOIUrl":"https://doi.org/10.1002/adhm.202500303","url":null,"abstract":"Bioconjugation is a pillar of modern medicine, enabling the likes of targeted therapeutics and sensitive diagnostics by exploiting synergies between biomolecules and functional materials. Conjugation techniques have expanded to match an evolving materials discovery landscape, fueling a new wave of bioconjugates. Despite the breadth of conjugate literature, most reviews describe common and relatively simple substrates such as metal nanoparticles or polymers. This review therefore centers around novel materials including biological (e.g., viral capsids, live cells), hybrid (e.g., gold-decorated nanoparticles, covalent-organic frameworks), and synthetic (e.g., piezoelectrics, upconverting nanoparticles) substrates. Applications in cancer and viral therapy, tissue engineering, optogenetics, antimicrobials, diagnostics, advanced imaging, and related topics are explored, revealing trends in conjugation approach. This review also compares characterization techniques used to confirm and optimize conjugation before offering perspectives on the future of the field. By shedding light on the latest materials, applications, and analytical methods, we hope to empower researchers to harness bioconjugation for transformative medical innovations.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e00303"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Cell-Based Tissues for Bottom-Up Assembly of Artificial Lymphatic Organs. 人工淋巴器官自下而上组装的合成细胞组织。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-23 DOI: 10.1002/adhm.202503498

Anna Burgstaller, Tamara Nink, Niklas Walter, Erick Angel Lopez Lopez, Hsin-Fang Chang, Oskar Staufer

{"title":"Synthetic Cell-Based Tissues for Bottom-Up Assembly of Artificial Lymphatic Organs.","authors":"Anna Burgstaller, Tamara Nink, Niklas Walter, Erick Angel Lopez Lopez, Hsin-Fang Chang, Oskar Staufer","doi":"10.1002/adhm.202503498","DOIUrl":"https://doi.org/10.1002/adhm.202503498","url":null,"abstract":"Synthetic cells have emerged as a novel biomimetic approach for studying fundamental cellular functions and enabling new therapeutic interventions. However, the potential to program synthetic cells into self-organized 3D collectives to replicate the structure and function of tissues has remained largely untapped. Here, self-assembly properties are engineered into synthetic cells to form millimeter-sized 3D lymphatic bottom-up tissues (lymphBUTs) with mechanical adaptability, metabolic activity, and hierarchical microstructural organization. It is demonstrated that primary human immune cells spontaneously infiltrate and functionally integrate into these synthetic lymph nodes to form living tissue hybrids. Applying lymphBUTs, it is shown that structured 3D organization and mechanical support drives T cell activation and the application of lymphBUTs for ex vivo expansion of regulatory CD8+ T cells is demonstrated. The study highlights the functional integration of living and non-living matter, advancing synthetic cell engineering toward 3D tissue structures.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03498"},"PeriodicalIF":9.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0