Advanced Healthcare Materials最新文献_第3页

New NIR-Activated Organic Molecule-Based Nanocomposite as an Efficient Sensitizer for Photothermal and Photodynamic Therapy of Cancer.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-02 DOI: 10.1002/adhm.202404418

Ming-Hsin Liu, Zhen-Jie Gao, Wei-Yung Huang, Chi-Hung Hsiao, Vincent Chen, Lee-Jene Lai, Zi-Jing Lin, Mo D-S Hua, Chia-Chun Hsieh, Er-Yuan Chuang, Jiashing Yu, Ken-Tsung Wong

{"title":"New NIR-Activated Organic Molecule-Based Nanocomposite as an Efficient Sensitizer for Photothermal and Photodynamic Therapy of Cancer.","authors":"Ming-Hsin Liu, Zhen-Jie Gao, Wei-Yung Huang, Chi-Hung Hsiao, Vincent Chen, Lee-Jene Lai, Zi-Jing Lin, Mo D-S Hua, Chia-Chun Hsieh, Er-Yuan Chuang, Jiashing Yu, Ken-Tsung Wong","doi":"10.1002/adhm.202404418","DOIUrl":"https://doi.org/10.1002/adhm.202404418","url":null,"abstract":"Acceptor-donor-acceptor (A-D-A)-configured molecules with coplanar dithieno[2,3-d:2',3'-d']thieno[3,2-b:3',2'-b']dipyrrole (DTPT) as the core are promising organic semiconductor materials utilized in organic photovoltaic devices owing to their efficient charge transportation capabilities. In addition to optoelectronic applications, they are potential in photothermal and photodynamic applications due to their light-absorption properties. This study evaluates the utilization of DTPT-based fused-ring-conjugated small molecules with strong near-infrared (NIR) absorption as stable organic photosensitizers for phototherapy by forming nanoparticles (NPs) with D-α-tocopherol polyethylene glycol 1000 succinate (TPGS). Among them, NPs prepared from the 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene)malononitrile (TCF) end-capping DTPT-centered molecule, DTPTTCF, exhibit low cytotoxicity, enhance photothermal conversion efficiency and superior photodynamic activity. In vitro and in vivo experiments demonstrate the remarkable anticancer efficacy of DTPTTCF@TPGS NPs that can effectively suppress cancer cell proliferation under 808 nm laser treatment. Additionally, soft X-ray tomography (SXT) is employed as a high-resolution tool to observe intracellular variations that reveal distinct vacuolization in the NPs + Laser treated group. These observations highlight that the DTPTTCF@TPGS NPs cause significant damage to cancer cells under NIR irradiation. Furthermore, in vivo, experiments demonstrate the apoptosis of cancer cells within tumor tissues and the effective elimination of tumors upon NIR irradiation of DTPTTCF@TPGS NPs treated mice. This work manifests the potential application of the DTPT-cored A-D-A-type molecule as an advanced agent for tumor phototherapy with enhanced efficacy and selectivity.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404418"},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering the Future of Restorative Clinical Peripheral Nerve Surgery.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202404293

Justin C Burrell, Zarina S Ali, Eric L Zager, Joseph M Rosen, Mykhailo M Tatarchuk, D Kacy Cullen

{"title":"Engineering the Future of Restorative Clinical Peripheral Nerve Surgery.","authors":"Justin C Burrell, Zarina S Ali, Eric L Zager, Joseph M Rosen, Mykhailo M Tatarchuk, D Kacy Cullen","doi":"10.1002/adhm.202404293","DOIUrl":"https://doi.org/10.1002/adhm.202404293","url":null,"abstract":"Peripheral nerve injury is a significant clinical challenge, often leading to permanent functional deficits. Standard interventions, such as autologous nerve grafts or distal nerve transfers, require sacrificing healthy nerve tissue and typically result in limited motor or sensory recovery. Nerve regeneration is complex and influenced by several factors: 1) the regenerative capacity of proximal neurons, 2) the ability of axons and support cells to bridge the injury, 3) the capacity of Schwann cells to maintain a supportive environment, and 4) the readiness of target muscles or sensory organs for reinnervation. Emerging bioengineering solutions, including biomaterials, drug delivery systems, fusogens, electrical stimulation devices, and tissue-engineered products, aim to address these challenges. Effective translation of these therapies requires a deep understanding of the physiology and pathology of nerve injury. This article proposes a comprehensive framework for developing restorative strategies that address all four major physiological responses in nerve repair. By implementing this framework, we envision a paradigm shift that could potentially enable full functional recovery for patients, where current approaches offer minimal hope.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404293"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanomaterial-Enhanced Biosensing: Mechanisms and Emerging Applications.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202500189

Younghak Cho, Yunyoung Choi, Yerim Jang, Hyejeong Seong

{"title":"Nanomaterial-Enhanced Biosensing: Mechanisms and Emerging Applications.","authors":"Younghak Cho, Yunyoung Choi, Yerim Jang, Hyejeong Seong","doi":"10.1002/adhm.202500189","DOIUrl":"https://doi.org/10.1002/adhm.202500189","url":null,"abstract":"Biosensors serve as indispensable analytical tools in biomedical diagnostics, environmental monitoring, and personalized healthcare, offering operation simplicity, cost-effectiveness, high sensitivity, and portability. Nanostructure integration has overcome traditional sensing platform limitations, particularly in sensitivity and response dynamics. These nanoscale materials-including nanoparticles, nanowires, nanosheets, and nanotubes-leverage unique physicochemical properties such as high surface-to-volume ratio, quantum confinement effects, and plasmonic interactions to enhance biosensor performance significantly. This review systematically analyzes recent advances in nanostructure-based biosensing technologies, examining how nanomaterial engineering improves sensor sensitivity, selectivity, and multifunctionality. Fundamental mechanisms are explored by which nanostructures enhance electrochemical, optical, and electrical biosensor performance, emphasizing low-abundance biomarkers in complex biological matrices. Beyond technological innovations, practical applications are evaluated across healthcare and environmental monitoring. Finally, current challenges and outline future research directions, highlighting these technologies' potential are addressed to transform diagnostic capabilities and healthcare outcomes.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2500189"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor-Targeted Exosome-Based Heavy Atom-Free Nanosensitizers With Long-Lived Excited States for Safe and Effective Sono-Photodynamic Therapy of Solid Tumors.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202500927

Van-Nghia Nguyen, Thuy Giang Nguyen Cao, Hyunsun Jeong, Quan Truong Hoang, Binh T T Pham, Jieun Bang, Chang Woo Koh, Ji Hee Kang, Jeong Hyun Lee, Xiaofeng Wu, Won Jong Rhee, Young Tag Ko, K M K Swamy, Sungnam Park, JaeHong Park, Min Suk Shim, Juyoung Yoon

{"title":"Tumor-Targeted Exosome-Based Heavy Atom-Free Nanosensitizers With Long-Lived Excited States for Safe and Effective Sono-Photodynamic Therapy of Solid Tumors.","authors":"Van-Nghia Nguyen, Thuy Giang Nguyen Cao, Hyunsun Jeong, Quan Truong Hoang, Binh T T Pham, Jieun Bang, Chang Woo Koh, Ji Hee Kang, Jeong Hyun Lee, Xiaofeng Wu, Won Jong Rhee, Young Tag Ko, K M K Swamy, Sungnam Park, JaeHong Park, Min Suk Shim, Juyoung Yoon","doi":"10.1002/adhm.202500927","DOIUrl":"https://doi.org/10.1002/adhm.202500927","url":null,"abstract":"Theranostic nanosensitizers with combined near-infrared (NIR) fluorescence imaging and sono-photodynamic effects have great potential for use in the personalized treatment of deep-seated tumors. However, developing effective nanosensitizers for NIR fluorescence image-guided sono-photodynamic therapy remains a considerable challenge, including the low generation efficacy of reactive oxygen species (ROS), poor photostability, and the absence of cancer specificity. Herein, a novel heavy atom-free nanosensitizer is developed, which exhibits intense NIR fluorescence, high ROS generation efficiency, and improved aqueous stability. By conjugating a bulky and electron-rich group, 4-(1,2,2-triphenylvinyl)-1,1'-biphenyl (TPE), to the IR820 backbone, the resulting IR820 bearing TPE (IR820-TPE) effectively generates ROS via type I and II photochemical mechanisms under 808 nm laser irradiation. Moreover, TPE conjugation considerably increases the sono-photodynamic performance of IR820. To improve the intracellular delivery and tumor-targeting ability of IR820-TPE, biotin-conjugated exosome (B-Exo) is used as a natural nanocarrier. In vitro studies demonstrate the outstanding therapeutic performance of IR820-TPE-loaded B-Exo (IR820-TPE@B-Exo) in synergistic sono-photodynamic cancer therapy. In vivo studies reveal that IR820-TPE@B-Exo shows enhanced tumor accumulation, strong fluorescence signals, and effective sono-photodynamic therapeutic activity with high biosafety. This work demonstrates that IR820-TPE@B-Exo is a promising sono-phototheranostic agent for safe and targeted cancer therapy and NIR fluorescence imaging.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2500927"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Modeling Studies of Hapten Design and Antibody Recognition for Sensitive Detection of Vitamin K3 by Strip Biosensor.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202404569

Jialin Hu, Xinxin Xu, Liqiang Liu, Aihua Qu, Hua Kuang, Chuanlai Xu

{"title":"Molecular Modeling Studies of Hapten Design and Antibody Recognition for Sensitive Detection of Vitamin K3 by Strip Biosensor.","authors":"Jialin Hu, Xinxin Xu, Liqiang Liu, Aihua Qu, Hua Kuang, Chuanlai Xu","doi":"10.1002/adhm.202404569","DOIUrl":"https://doi.org/10.1002/adhm.202404569","url":null,"abstract":"Computer-aided molecular design techniques are used to analyze vitamin K3 (VK3) and VK3-hapten. Based on VK3-hapten, a specific monoclonal antibody (mAb) against VK3 is prepared with a sensitivity (IC50) of 0.49 ng mL-1. The recombinant technology is used to investigate the molecular docking mechanism of mAb recognition of VK3. Then, a model of the mAb is established, and the amino acid distributions of the complementarity determining region regions of the mAb are determined. Hydrogen bonding and hydrophobic interactions of specific amino acids of the mAb are further confirmed by the recognition mechanism of the antibody with VK3. Based on these results, a gold immunochromatographic assay (GICA) is developed to detect VK3. The recovery of VK3 in the sample is 99.50%-101.12%, showing a better agreement with the results of the high-performance liquid chromatography. In addition, the calculated limit of detection of VK3 in milk powder, vitamin tablets, and mixed animal feed is 1.16, 1.18, and 10.06 µg kg-1, respectively. The concentrations of VK3 in vitamin tablets and mixed animal feed as determined with the GICA strips are 5.82 mg/tablet and 1.47 mg kg-1, respectively. These results confirmed that the developed GICA strips have great potential for detecting VK3 in actual samples.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404569"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lighting up Resistance: Rapid Antimicrobial Susceptibility Testing of Gram-Negative Bacteria in Bloodstream Infections Using an Aggregation-Induced Emission Bioprobe.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202405300

Xiaoxue Ge, Meng Gao, Nannan Cao, Meiwei Mou, Shujuan Guan, Bairong He, Xiumei Hu, Bo Situ, Lei Zheng

{"title":"Lighting up Resistance: Rapid Antimicrobial Susceptibility Testing of Gram-Negative Bacteria in Bloodstream Infections Using an Aggregation-Induced Emission Bioprobe.","authors":"Xiaoxue Ge, Meng Gao, Nannan Cao, Meiwei Mou, Shujuan Guan, Bairong He, Xiumei Hu, Bo Situ, Lei Zheng","doi":"10.1002/adhm.202405300","DOIUrl":"https://doi.org/10.1002/adhm.202405300","url":null,"abstract":"Rapid and accurate antimicrobial susceptibility testing (AST) is crucial for guiding treatment and combating resistance. However, conventional ASTs are time-consuming and require pure colonies, delaying the initiation of targeted antimicrobial therapy. Herein, a novel AST based on an aggregation-induced emission luminogen (AIEgen), DATVP, which can directly assess the antimicrobial susceptibility of Gram-negative bacteria in positive blood cultures, is reported. DATVP specifically lights up Gram-negative bacteria with damaged cell membranes while showing no fluorescence in intact bacteria. The antimicrobial-induced fluorescence turn-on of DATVP is found to be fast (within 6 h) and sensitive, allowing for reliable determination of antimicrobial susceptibility. Using DATVP, a wash-free AST is developed and its performance was validated on clinical isolates. The DATVP-based AST showed high categorical agreement (84-95%) with the standard method while shortening the time-to-result from days to hours. This method represents a new paradigm in phenotypic AST, offering speed, simplicity, and direct applicability to patient samples, with the potential to enable timely and targeted antimicrobial treatment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2405300"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil Membrane-Encapsulated Polymerized Salicylic Acid Nanoparticles Effectively Alleviating Rheumatoid Arthritis by Facilitating Sustained Release of Salicylic Acid into the Articular Cavity from Chondrocytes.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-04-01 DOI: 10.1002/adhm.202404510

Luying Yang, Feng Cao, Jiayu Lu, Simo Wu, Le Wang, Jianzhen She, Boling He, Xiaoying Xu, Fan Shi, Ye Gao, Zhou Ye, Baolin Guo, Liang Kong, Ronghua Jin, Bolei Cai

{"title":"Neutrophil Membrane-Encapsulated Polymerized Salicylic Acid Nanoparticles Effectively Alleviating Rheumatoid Arthritis by Facilitating Sustained Release of Salicylic Acid into the Articular Cavity from Chondrocytes.","authors":"Luying Yang, Feng Cao, Jiayu Lu, Simo Wu, Le Wang, Jianzhen She, Boling He, Xiaoying Xu, Fan Shi, Ye Gao, Zhou Ye, Baolin Guo, Liang Kong, Ronghua Jin, Bolei Cai","doi":"10.1002/adhm.202404510","DOIUrl":"https://doi.org/10.1002/adhm.202404510","url":null,"abstract":"Rheumatoid arthritis (RA) is a systemic autoimmune disease that primarily instigates chronic inflammation in multiple joints. Salicylic acid (SA) is a classic anti-inflammatory agent for the treatment of RA. To enhance the therapeutic effect of SA, an innovative therapeutic approach for RA is developed by encapsulating polymerized-SA (PSA) nanoparticles within neutrophil membranes. The study demonstrated that neutrophil membranes endowed PSAs with the ability to selectively target inflammatory joints in RA mice, where they specifically accumulated within the inflammatory chondrocytes. The internalized PSAs underwent gradual degradation into SA within chondrocytes, facilitating sustained release into the articular cavity and effectively alleviating RA symptoms. By attenuating the expression of inflammatory mediators within the joint cavity and suppressing neutrophil extracellular traps (NETs) in the synovium, neutrophil membrane encapsulated polymerized salicylic acid nanoparticles (N-PSAs) effectively restore long-term intra-articular homeostasis in RA mice, thereby establishing a conducive microenvironment for cartilage repair. In summary, the articular chondrocytes represent an optimal reservoir for therapeutic agents targeting joint disorders. By conferring PSA with the capability to specifically target inflammatory chondrocytes, the neutrophil membrane-coated drug-polymerized nanoparticles offer a promising therapeutic strategy for the management of rheumatoid arthritis (RA) and serve as a valuable reference for treating other inflammatory joint disorders.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404510"},"PeriodicalIF":10.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Mode Radiosensitization of Esophageal Squamous Cell Carcinoma via SOCS6-Loaded Virus-Inspired Manganese-Bismuth Bimetallic Oxide Nanoparticles.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-03-31 DOI: 10.1002/adhm.202404737

Rui Ma, Zhi Yang, Xia Miao, Jing Hu, Te Zhang, Li-Tian Ma, Jin-Yan Lin, Li-Na Zhao

{"title":"Dual-Mode Radiosensitization of Esophageal Squamous Cell Carcinoma via SOCS6-Loaded Virus-Inspired Manganese-Bismuth Bimetallic Oxide Nanoparticles.","authors":"Rui Ma, Zhi Yang, Xia Miao, Jing Hu, Te Zhang, Li-Tian Ma, Jin-Yan Lin, Li-Na Zhao","doi":"10.1002/adhm.202404737","DOIUrl":"https://doi.org/10.1002/adhm.202404737","url":null,"abstract":"Radioresistance poses a significant obstacle to controlling the recurrence of esophageal squamous cell carcinoma (ESCC) during radiotherapy. It is urgent to develop innovative radiosensitization strategies to improve the prognosis of patients with ESCC. Here, a novel dual-mode radiosensitizer: a virus-inspired hollow mesoporous manganese-bismuth bimetallic oxide nanoparticles (vHMMn-Bi) encapsulating the radiosensitizing plasmids (suppressor of cytokine signaling 6, SOCS6) is developed, designed to significantly amplify ESCC radiotherapy under hypoxic conditions. After intravenous injection, the SOCS6@vHMMn-Bi nanoparticles can be efficiently delivered to the tumor site and rapidly invade tumor cells by virus-like surface-assisted adhesion. Under X-ray irradiation, the nanoparticles exhibits a unique dual-mode sensitization effect, encompassing exogenous and endogenous mechanisms, thereby significantly augmenting the ESCC radiotherapeutic effectiveness. First, the Bi2O3 within the shell can enhance the radiosensitivity owing to its robust X-ray attenuation characteristics. Second, the SOCS6 released from the interior can inhibit both HIF-1α and JAK2/STAT3 signaling pathways, triggering ROS upregulation and intensifying radiation-mediated DNA damage inside ESCC cells. Furthermore, the shell employs MnO2 to catalyze the decomposition of endogenous H2O2 to increase oxygen generation, alleviating hypoxia within the tumor microenvironment. These nanoparticles demonstrates considerable potential as dual-mode radiosensitizers with no systemic toxicity and low immunogenicity for amplifying radiotherapeutic efficacy in ESCC.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404737"},"PeriodicalIF":10.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable-Dynamic Hydrogels Mimicking the Pericellular Matrix for Articular Cartilage Repair.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-03-30 DOI: 10.1002/adhm.202405081

Yixin Li, Tiancheng Li, Cheng Zhu, Hairui Li, Runzhe Fang, Ruomei Li, Yu Jin, Zhiyu Zhu, Lunguo Xia, Bing Fang

{"title":"Stable-Dynamic Hydrogels Mimicking the Pericellular Matrix for Articular Cartilage Repair.","authors":"Yixin Li, Tiancheng Li, Cheng Zhu, Hairui Li, Runzhe Fang, Ruomei Li, Yu Jin, Zhiyu Zhu, Lunguo Xia, Bing Fang","doi":"10.1002/adhm.202405081","DOIUrl":"https://doi.org/10.1002/adhm.202405081","url":null,"abstract":"Cartilage regeneration requires a specialized biomechanical environment. Macroscopically, cartilage repair requires a protracted, stable mechanical environment, whereas microscopically, it involves dynamic interactions between cells and the extracellular matrix. Therefore, this study aims to design a hydrogel that meets the complex biomechanical requirements for cartilage repair. Dynamic hybrid hydrogels with temporal stability at the macroscale and dynamic properties at the microscale are successfully synthesized. The dynamic hybrid hydrogel simulates the stress relaxation and viscoelasticity of the pericellular matrix, facilitating effective interactions between the extracellular matrix and cells. The in vitro and in vivo experiments demonstrated that the hybrid hydrogel significantly promoted cartilage repair. The dynamic hybrid hydrogel alleviates abnormal actin polymerization, reduces intracellular stress, and increases the volume of individual cells. By modulating the cytoskeleton, the hybrid hydrogel inhibits Notch signal transduction in both the receptor and ligand cells, resulting in an improved cartilage phenotype. This study introduces an effective hybrid hydrogel scaffold that modulates the chondrocyte cytoskeleton and Notch signaling pathways by establishing an appropriate biomechanical environment, thus offering a promising material for cartilage repair.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2405081"},"PeriodicalIF":10.0,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging the Shape Fidelity of 3D Printed Bone Scaffolds Through Architectural Tailoring of an Emulsion Ink: A Combined Experimental and Computational Analysis.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-03-30 DOI: 10.1002/adhm.202404866

Sagnik Ghosh, Shubham Shankar Mohol, Anupama Datta, Pulak Mohan Pandey, Ritu Kulshreshtha, Bhanu Nandan, Minna Hakkarainen, Rajiv K Srivastava

{"title":"Leveraging the Shape Fidelity of 3D Printed Bone Scaffolds Through Architectural Tailoring of an Emulsion Ink: A Combined Experimental and Computational Analysis.","authors":"Sagnik Ghosh, Shubham Shankar Mohol, Anupama Datta, Pulak Mohan Pandey, Ritu Kulshreshtha, Bhanu Nandan, Minna Hakkarainen, Rajiv K Srivastava","doi":"10.1002/adhm.202404866","DOIUrl":"https://doi.org/10.1002/adhm.202404866","url":null,"abstract":"Hierarchical porous, bioactive, and biocompatible scaffolds with customizable multi-functionality are promising alternatives for autografts and allografts in bone tissue engineering. Combining high internal phase emulsion (HIPE) templating with additive manufacturing provides possibilities to produce such multiscale porous scaffolds. 3D printing of HIPE remains a challenging task due to the intense phase separation under high shear extrusion and reported printability (Pr) of either less than or greater than 1. Tuning viscoelastic properties of emulsion is therefore required to achieve a Pr ≈1. This study addresses these issues by preparing Pickering HIPEs using dual networks with synergistic viscous and elastic properties, stabilized by Cloisite 30B interphase. This configuration enhances viscoelasticity and achieves Pr values close to 1 (0.98-1.02). The printed scaffolds exhibit trabecular bone-like, hierarchical interconnected porosity (77%-86%). Computational simulations accurately predict the mechanical, biological, and degradation behavior. Functionalization with Cissus quadrangularis bioactivates the scaffolds, demonstrates in vivo biocompatibility, promotes MC3T3-E1 adhesion, and proliferation, accelerates osteogenesis, and reduces oxidative stress compared to neat PCL scaffolds. This work introduces a facile strategy for \"engineering printability\" to produce regenerative materials with hierarchical design and holds the potential for developing optimized bone tissue engineering scaffolds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404866"},"PeriodicalIF":10.0,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0