Advanced Healthcare Materials最新文献_第3页

Fe/Cu Bimetallic Nanozyme Co-Assembled with ¹⁷⁷Lu and Tanshinone for Quadruple-Synergistic Tumor-Specific Therapy. 铁/铜双金属纳米酶与 177Lu 和丹参酮共同组装，用于四重协同肿瘤特异性治疗。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-14 DOI: 10.1002/adhm.202402696

Xianyu Zhu, Lingling Zheng, Pengfei Zhao, Lingli Gao, Liang Wang, Jun Liu, Xu Yang, Hanrui Wei, Mingyu Zhang, Liang Yan, Han Lv, Jianhua Gong, Ji Gang Yang, Zhenchang Wang

{"title":"Fe/Cu Bimetallic Nanozyme Co-Assembled with 177Lu and Tanshinone for Quadruple-Synergistic Tumor-Specific Therapy.","authors":"Xianyu Zhu, Lingling Zheng, Pengfei Zhao, Lingli Gao, Liang Wang, Jun Liu, Xu Yang, Hanrui Wei, Mingyu Zhang, Liang Yan, Han Lv, Jianhua Gong, Ji Gang Yang, Zhenchang Wang","doi":"10.1002/adhm.202402696","DOIUrl":"https://doi.org/10.1002/adhm.202402696","url":null,"abstract":"The co-loading of radionuclides and small-molecule chemotherapeutic drugs as nanotheranostic platforms using nanozymes holds tremendous potential for imaging-guided synergistic therapy. This study presents such nanotheranostic platform (177Lu-MFeCu@Tan) via co-assembling 177Lu radionuclide and tanshinone (Tan) into Fe/Cu dual-metal nanozyme (MFeCu). This platform simultaneously enables single-photon emission computed tomography (SPECT) imaging and a quadruple-synergistic tumor therapy approach, including internal radioisotope therapy (RIT), catalysis therapy, chemotherapy, and MFeCu-mediated ferroptosis and cuproptosis therapy. In this platform, the MFeCu can catalyze excessive intracellular hydrogen peroxide (H2O2) to generate radical oxygen species (ROS) and deplete glutathione (GSH). The excess of H2O2 and GSH are main factors for radioresistance and chemoresistance, reducing them can enhance chemotherapy and RIT. The generated ROS and depleted GSH further induce mitochondrial dysfunction and promote the aggregation of lipoylated dihydrolipoamide S-acetyltransferase and lipid peroxidation, causing the enhance of ferroptosis and cuproptosis. The in vitro and in vivo results demonstrate that this quadruple-synergistic approach shows significant therapeutic efficacy to complete tumor eradication and reduced recurrence in vivo. In conclusion, this work presents a promising strategy for designing SPECT imaging-guided quadruple-synergistic therapy and highlights the feasibility of developing a self-assembled radionuclide and small molecule chemotherapy drugs nanotherapeutic platform for combined treatment of cancer.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402696"},"PeriodicalIF":10.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel Elastic Energy: A Stressor Triggering an Adaptive Stress-Mediated Cell Response. 水凝胶弹性能：触发适应性应力介导细胞反应的应激源

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-13 DOI: 10.1002/adhm.202402400

Sara Lipari, Pasquale Sacco, Michela Cok, Francesca Scognamiglio, Maurizio Romano, Francesco Brun, Piero Giulio Giulianini, Eleonora Marsich, Finn L Aachmann, Ivan Donati

{"title":"Hydrogel Elastic Energy: A Stressor Triggering an Adaptive Stress-Mediated Cell Response.","authors":"Sara Lipari, Pasquale Sacco, Michela Cok, Francesca Scognamiglio, Maurizio Romano, Francesco Brun, Piero Giulio Giulianini, Eleonora Marsich, Finn L Aachmann, Ivan Donati","doi":"10.1002/adhm.202402400","DOIUrl":"https://doi.org/10.1002/adhm.202402400","url":null,"abstract":"The crosstalk between the cells and the extracellular matrix (ECM) is bidirectional and consists of a pushing/pulling stretch exerted by the cells and a mechanical resistance counteracted by the surrounding microenvironment. It is widely recognized that the stiffness of the ECM, its viscoelasticity, and its overall deformation are the most important traits influencing the response of the cells. Here these three parameters are combined into a concept of elastic energy, which in biological terms represents the mechanical feedback that cells perceive when the ECM is deformed. It is shown that elastic energy is a stress factor that influences the response of cells in three-dimensional (3D) cultures. Strikingly, the higher the elastic energy of the matrix and thus the mechanical feedback, the higher the stress state of the cells, which correlates with the formation of G3BP-mediated stress granules. This condition is associated with an increase in alkaline phosphatase (ALP) activity but a decrease in gene expression and is mediated by the nuclear translocation of Yes-associated protein (YAP). This work supports the importance of considering the elastic energy as mechano-controller in regulating cellular stress state in 3D cultures.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402400"},"PeriodicalIF":10.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumoroid-On-a-Plate (ToP): Physiologically Relevant Cancer Model Generation and Therapeutic Screening. Tumoroid-On-a-Plate (ToP)：与生理相关的癌症模型生成和治疗筛选。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-13 DOI: 10.1002/adhm.202402060

Amir Seyfoori, Kaiwen Liu, Hector J Caruncho, Patrick B Walter, Mohsen Akbari

{"title":"Tumoroid-On-a-Plate (ToP): Physiologically Relevant Cancer Model Generation and Therapeutic Screening.","authors":"Amir Seyfoori, Kaiwen Liu, Hector J Caruncho, Patrick B Walter, Mohsen Akbari","doi":"10.1002/adhm.202402060","DOIUrl":"https://doi.org/10.1002/adhm.202402060","url":null,"abstract":"Employing three-dimensional (3D) in vitro models, including tumor organoids and spheroids, stands pivotal in enhancing cancer therapy. These models bridge the gap between two-dimensional (2D) cell cultures and complex in vivo environments and offer versatile tools for comprehensive studies into cancer progression, drug responses, and tailored therapies. This study introduces the Tumoroid-on-a-Plate (ToP) device, an innovative ope-surface microfluidic platform designed to create predictive 3D models of solid tumors. The ToP device combines tumor mass, stromal cells, and extracellular matrix (ECM) components, to closely replicate the microenvironment of glioblastoma (GBM) and pancreatic adenocarcinoma (PDAC). Using the advanced ToP model and testing various GBM ECM compositions such as collagen and Rreelin within the model, we can assess how specific elements affect GBM invasiveness. The ToP in vitro model also enables screening chemotherapeutics such as temozolomide and iron-chelators in a single and binary treatment setting on the complex ECM-embedded tumoroids to evaluate their toxicity on GBM and PDAC models viability and apoptosis. Furthermore, co-culturing PDAC tumoroids with human-derived fibroblasts reveals the pro-invasive influence of stromal elements on tumor growth and drug response. This research underscores the value of advanced 3D models like ToP in advancing the understanding of cancer complexity and therapy responses.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402060"},"PeriodicalIF":10.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual Antibacterial Property and Selectivity Enabled by Tuning Nanozyme Activities of L-Arginine Derived Carbon Dots. 通过调节 L-精氨酸衍生碳点的纳米酶活性实现非同寻常的抗菌特性和选择性。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-13 DOI: 10.1002/adhm.202403201

Meizhe Yu, Peili Li, Jiaying Li, Xueli Chen, Zhimin Hu, Yiran Wang, Jing Zeng, Fengxuan Han, Xuedong Gong, Bin Li, Xiaodong Xing

{"title":"Unusual Antibacterial Property and Selectivity Enabled by Tuning Nanozyme Activities of L-Arginine Derived Carbon Dots.","authors":"Meizhe Yu, Peili Li, Jiaying Li, Xueli Chen, Zhimin Hu, Yiran Wang, Jing Zeng, Fengxuan Han, Xuedong Gong, Bin Li, Xiaodong Xing","doi":"10.1002/adhm.202403201","DOIUrl":"https://doi.org/10.1002/adhm.202403201","url":null,"abstract":"Functional integration of antimicrobial activity and cell proliferation promotion at low concentrations is important for the clinical application of carbon dots (CDs). In this study, the precursor, L-arginine, and dopant, copper salt, are used to prepare copper-doped CDs (Cu-CDs). Owing to their excellent synergistic enzyme-like activities, Cu-CDs can rapidly increase reactive oxygen species (ROS) to lethal levels, preferentially in bacteria, and exhibit potent antibacterial ability, which can mainly be attributed to the membrane disruption effect. Concurrently, the cell proliferation-promoting activity of arginine-derived CDs is inherited. The Cu-CDs achieve perfect integration of dual functions at low concentrations, especially advantageous for applications. With as little as 100 µg mL-1 of Cu-CDs, the infected wound heals obviously faster than 2 mg mL-1 of antibiotic, although the traditional antibiotic group shows slightly better antibacterial efficiency, suggesting its effect in simultaneously scavenging bacteria and promoting tissue repair effect in vivo. The super selective mechanism probably originates from the endocytosis of Cu-CDs by mammalian cells, while superoxide dismutase down-regulates ROS levels in cells to act as a mitotic signaling agent for promoting cell growth. This strategy provides an efficient, convenient, and safe solution to combat bacterial infections, and suggests a novel approach for modifying antimicrobial biomaterials.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403201"},"PeriodicalIF":10.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of Ferroptosis Inhibition and Immune Evasion with Tumor-Activatable Prodrug for Boosted Photodynamic/Chemotherapy Eradication of Drug-Resistant Tumors. 利用可激活肿瘤的原药破坏铁凋亡抑制和免疫逃避，促进光动力/化学疗法根除耐药肿瘤。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-12 DOI: 10.1002/adhm.202403473

Tao Bi, Qixin Zhao, Ting Wang, Rui Huang, Bangguo Liu, Xinyue Liu, Yihuan Wang, Qin Sun, Yingcheng Yang, Zengjin Liu

{"title":"Disruption of Ferroptosis Inhibition and Immune Evasion with Tumor-Activatable Prodrug for Boosted Photodynamic/Chemotherapy Eradication of Drug-Resistant Tumors.","authors":"Tao Bi, Qixin Zhao, Ting Wang, Rui Huang, Bangguo Liu, Xinyue Liu, Yihuan Wang, Qin Sun, Yingcheng Yang, Zengjin Liu","doi":"10.1002/adhm.202403473","DOIUrl":"https://doi.org/10.1002/adhm.202403473","url":null,"abstract":"Breast cancer is a malignant tumor that threatens the life and health of women worldwide. As the first-line chemotherapy drug for breast cancer, doxorubicin (DOX) can inhibit the synthesis of RNA and DNA, and it exhibits strong inhibitory activity against breast cancer. However, drug-induced systemic toxicity and drug resistance can occur with DOX treatment. In this work, TSPO protein is identified as a promising target for overcoming drug resistance and we designed a novel BT-DOX/PDP conjugate to solve these problems in drug chemotherapy. It is found that BT-DOX/PDP can effectively downregulate TSPO1 protein and sensitize MCF-7/Adr to DOX. Furthermore, due to its positive charge, BT-DOX/PDP is readily loaded into puerarin (PUE), the resulting BT-DOX/PDP@PUE exhibited minimal systemic toxicity but enhanced antitumor activity in animal models, as compared with BT-DOX/PDP. This study demonstrates the advantages of combined chemotherapy and photodynamic therapy in overcoming drug resistance, which may be applied in the design of other photodynamic therapy-based conjugates to enhance antitumor therapy.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403473"},"PeriodicalIF":10.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low Molecular Weight Alginate Oligosaccharides as Alternatives to PEG for Enhancement of the Diffusion of Cationic Nanoparticles Through Cystic Fibrosis Mucus. 低分子量海藻酸寡糖作为 PEG 的替代品，用于增强阳离子纳米粒子在囊性纤维化粘液中的扩散。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-12 DOI: 10.1002/adhm.202400510

Ruhina Maeshima, Aristides D Tagalakis, Dafni Gyftaki-Venieri, Stuart A Jones, Philip D Rye, Anne Tøndervik, O Alexander H Åstrand, Stephen L Hart

{"title":"Low Molecular Weight Alginate Oligosaccharides as Alternatives to PEG for Enhancement of the Diffusion of Cationic Nanoparticles Through Cystic Fibrosis Mucus.","authors":"Ruhina Maeshima, Aristides D Tagalakis, Dafni Gyftaki-Venieri, Stuart A Jones, Philip D Rye, Anne Tøndervik, O Alexander H Åstrand, Stephen L Hart","doi":"10.1002/adhm.202400510","DOIUrl":"https://doi.org/10.1002/adhm.202400510","url":null,"abstract":"Airway mucus is a major barrier to the delivery of lipid-based nanoparticles in chronic airway diseases such as cystic fibrosis (CF). Receptor-Targeted Nanocomplexes (RTN), comprise mixtures of cationic lipids and bifunctional peptides with receptor-targeting and nucleic acid packaging properties. The aim of this study is to improve the mucus-penetrating properties of cationic siRNA and mRNA RTNs by combining them with low molecular weight alginate oligosaccharides, OligoG and OligoM. Cationic RTNs formulated with either alginate become strongly anionic, while PEGylated messenger RNA (mRNA) and short interfering RNA (siRNA) RTNs remain cationic. Both alginates enhance mucus diffusion rates of cationic siRNA and mRNA RTNs in a static mucus barrier diffusion model, with OligoG particularly effective. PEGylation also enhance mucus diffusion rates of siRNA RTNs but not mRNA RTNs. Electron microscopy shows that RTNs remained intact after mucosal transit. The transfection efficiency of OligoM-coated mRNA RTNs is better than those coated with OligoG or PEG, and similar to cationic RTNs. In siRNA RTN transfections, OligoM is better than OligoG although 1% PEG is slightly better than both. The combination of cationic RTNs and alginate oligosaccharides represents a promising alternative to PEGylation for epithelial delivery of genetic therapies across the mucus barrier while retaining transfection efficiency.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2400510"},"PeriodicalIF":10.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable Scaffolds. 用于可酶降解支架的聚羟基烷酸酯熔融电泳。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-12 DOI: 10.1002/adhm.202401504

Magdalena Z Gładysz, Didi Ubels, Marcus Koch, Armin Amirsadeghi, Frederique Alleblas, Sander van Vliet, Marleen Kamperman, Jeroen Siebring, Anika Nagelkerke, Małgorzata K Włodarczyk-Biegun

{"title":"Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable Scaffolds.","authors":"Magdalena Z Gładysz, Didi Ubels, Marcus Koch, Armin Amirsadeghi, Frederique Alleblas, Sander van Vliet, Marleen Kamperman, Jeroen Siebring, Anika Nagelkerke, Małgorzata K Włodarczyk-Biegun","doi":"10.1002/adhm.202401504","DOIUrl":"https://doi.org/10.1002/adhm.202401504","url":null,"abstract":"Melt electrowriting (MEW) enables precise scaffold fabrication for biomedical applications. With a limited number of processable materials with short and tunable degradation times, polyhydroxyalkanoates (PHAs) present an interesting option. Here, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a blend of PHBV and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHBV+P34HB) are successfully melt electrowritten into scaffolds with various architectures. PHBV+P34HB exhibits greater thermal stability, making it a superior printing material compared to PHBV in MEW. The PHBV+P34HB scaffolds subjected to enzymatic degradation show tunable degradation times, governed by enzyme dilution, incubation time, and scaffold surface area. PHBV+P34HB scaffolds seeded with human dermal fibroblasts (HDFs), demonstrate enhanced cell adherence, proliferation, and spreading. The HDFs, when exposed to the enzyme solutions and enzymatic degradation residues, show good viability and proliferation rates. Additionally, HDFs grown on enzymatically pre-incubated scaffolds do not show any difference in behavior compared those grown on control scaffolds. It is concluded that PHAs, as biobased materials with enzymatically tunable degradability rates, are an important addition to the already limited set of materials available for MEW technology.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2401504"},"PeriodicalIF":10.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmable Nanomodulators for Precision Therapy, Engineering Tumor Metabolism to Enhance Therapeutic Efficacy. 用于精准治疗的可编程纳米调节剂，通过肿瘤代谢工程提高疗效。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-12 DOI: 10.1002/adhm.202403019

Siwei Liu, Zhijun Liu, Huajiang Lei, Yang-Bao Miao, Jiao Chen

{"title":"Programmable Nanomodulators for Precision Therapy, Engineering Tumor Metabolism to Enhance Therapeutic Efficacy.","authors":"Siwei Liu, Zhijun Liu, Huajiang Lei, Yang-Bao Miao, Jiao Chen","doi":"10.1002/adhm.202403019","DOIUrl":"https://doi.org/10.1002/adhm.202403019","url":null,"abstract":"Tumor metabolism is crucial in the continuous advancement and complex growth of cancer. The emerging field of nanotechnology has made significant strides in enhancing the understanding of the complex metabolic intricacies inherent to tumors, offering potential avenues for their strategic manipulation to achieve therapeutic goals. This comprehensive review delves into the interplay between tumor metabolism and various facets of cancer, encompassing its origins, progression, and the formidable challenges posed by metastasis. Simultaneously, it underscores the classification of programmable nanomodulators and their transformative impact on enhancing cancer treatment, particularly when integrated with modalities such as chemotherapy, radiotherapy, and immunotherapy. This review also encapsulates the mechanisms by which nanomodulators modulate tumor metabolism, including the delivery of metabolic inhibitors, regulation of oxidative stress, pH value modulation, nanoenzyme catalysis, nutrient deprivation, and RNA interference technology, among others. Additionally, the review delves into the prospects and challenges of nanomodulators in clinical applications. Finally, the innovative concept of using nanomodulators to reprogram metabolic pathways is introduced, aiming to transform cancer cells back into normal cells. This review underscores the profound impact that tailored nanomodulators can have on tumor metabolic, charting a path toward pioneering precision therapies for cancer.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403019"},"PeriodicalIF":10.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilayer Scaffolds Synergize Immunomodulation and Rejuvenation via Layer-Specific Release of CK2.1 and the "Exercise Hormone" Lac-Phe for Enhanced Osteochondral Regeneration. 双层支架通过层特异性释放 CK2.1 和 "运动荷尔蒙 "Lac-Phe 来协同免疫调节和再生，从而增强骨软骨再生。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-12 DOI: 10.1002/adhm.202402329

Po-Lin Liu, Shu-Hang He, Zhi-Han Shen, Xu-Ran Li, Qing-Song Deng, Zhan-Ying Wei, Chang-Ru Zhang, Xiao-Qiu Dou, Tong-He Zhu, Helen Dawes, Jian Lu, Shang-Chun Guo, Shi-Cong Tao

{"title":"Bilayer Scaffolds Synergize Immunomodulation and Rejuvenation via Layer-Specific Release of CK2.1 and the \"Exercise Hormone\" Lac-Phe for Enhanced Osteochondral Regeneration.","authors":"Po-Lin Liu, Shu-Hang He, Zhi-Han Shen, Xu-Ran Li, Qing-Song Deng, Zhan-Ying Wei, Chang-Ru Zhang, Xiao-Qiu Dou, Tong-He Zhu, Helen Dawes, Jian Lu, Shang-Chun Guo, Shi-Cong Tao","doi":"10.1002/adhm.202402329","DOIUrl":"https://doi.org/10.1002/adhm.202402329","url":null,"abstract":"Repairing osteochondral defects necessitates the intricate reestablishment of the microenvironment. The cartilage layer consists of a porous gelatin methacryloyl hydrogel (PGelMA) covalently crosslinked with the chondroinductive peptide CK2.1 via a \"linker\" acrylate-PEG-N-hydroxysuccinimide (AC-PEG-NHS). This layer is optimized for remodeling the senescent microenvironment in the cartilage region, thereby establishing a regenerative microenvironment that supports chondrogenesis. For the bone layer, silk fibroin methacryloyl (SilMA) is coated onto a three dimensional (3D)-printed 45S5 bioactive glass scaffold (BG scaffold). The \"exercise hormone\" N-lactoyl-phenylalanine (Lac-Phe) is loaded onto the SilMA, endowing it with diversified functions to regulate the osteogenic microenvironment. Systematic analysis in vitro reveals that PGelMA-CK2.1 shifts the microenvironment from a pro-inflammatory into an anti-inflammatory condition, and alleviates cellular senescence, thus modifying the cartilage microenvironment to improve the recruitment, proliferation and chondral differentiation of bone marrow mesenchymal stem cells (BMSCs). The scaffold bone layer enhances microvascular endothelial cell proliferation, migration, and angiogenic activities, which, couple with increased BMSC recruitment and regulatory mechanisms directing BMSC differentiation, favor a shift in the \"osteogenesis-adipogenesis\" balance toward enhanced osteogenesis. In vivo, it is found that this biphasic biomimetic scaffold favors simultaneous dual tissue regeneration. This approach facilitates the development of bioactive regenerative scaffolds and holds great potential for clinical application.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402329"},"PeriodicalIF":10.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting TUBB3 Suppresses Anoikis Resistance and Bone Metastasis in Prostate Cancer (Adv. Healthcare Mater. 28/2024) 靶向 TUBB3 可抑制前列腺癌的 Anoikis 抗性和骨转移（Adv.）

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-11 DOI: 10.1002/adhm.202470181

Bingqi Dong, Yanlun Gu, Xiaojiao Sun, Xin Wang, Ying Zhou, Zhuona Rong, Jixin Zhang, Xuedong Shi, Zhuo Zhang, Xu He, Lin Chen, Qingqing Xiong, Xiaocong Pang, Yimin Cui

引用次数: 0