Advanced Healthcare Materials最新文献_第4页

Pre-Clinical Assessment of Bupivacaine-Loaded Poly(ester urea) Thin Films for Controlled Drug Release and Effective Pain Management After Surgery.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-12 DOI: 10.1002/adhm.202402800

Natasha C Stinson, Yutaka Matsuoka, Anshu Agarwal, Courtney S Dziewior, Samantha M McDonald, Yize Li, Kacey Godwin, Ru-Rong Ji, Matthew L Becker

{"title":"Pre-Clinical Assessment of Bupivacaine-Loaded Poly(ester urea) Thin Films for Controlled Drug Release and Effective Pain Management After Surgery.","authors":"Natasha C Stinson, Yutaka Matsuoka, Anshu Agarwal, Courtney S Dziewior, Samantha M McDonald, Yize Li, Kacey Godwin, Ru-Rong Ji, Matthew L Becker","doi":"10.1002/adhm.202402800","DOIUrl":"https://doi.org/10.1002/adhm.202402800","url":null,"abstract":"Safe, effective pain management remains one of the biggest challenges following surgical procedures. Despite widespread recognition of this problem and advances in the mechanistic understanding of pain signaling, post-surgical pain is often undermanaged, with opioid use remaining the clinical standard. As an alternative to current oral, systemic treatments, a degradable bupivacaine-loaded poly(ester urea) (PEU) thin film has been developed to deliver bupivacaine directly to the site of injury over an extended duration. The dose and duration of bupivacaine delivery is controlled using polymer composition and bupivacaine concentration. Systemic bupivacaine concentrations are more than an order of magnitude lower when delivered locally versus intravenous injection. Tissue analysis showed that the majority of bupivacaine is deposited into subcutaneous tissue directly surrounding the implant. Bupivacaine concentration in soft tissue around the implant are 30-fold higher than plasma values, indicating that release from PEU implants remains localized. Bupivacaine-loaded PEU films are assessed into two established mouse models for diabetic neuropathic pain and post-surgical incisional pain. In each model, bupivacaine eluting PEU films effectively block pain for 3-5 days before returning to baseline levels without loss of motor function and without signs of neurotoxicity.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402800"},"PeriodicalIF":10.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-Ear Electronics with Mechanical Adaptability for Physiological Sensing.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202404296

Shuyun Zhuo, Zihuan Wu, Chris Williams, Chithiravel Sundaresan, Shideh Kabiri Ameri

{"title":"In-Ear Electronics with Mechanical Adaptability for Physiological Sensing.","authors":"Shuyun Zhuo, Zihuan Wu, Chris Williams, Chithiravel Sundaresan, Shideh Kabiri Ameri","doi":"10.1002/adhm.202404296","DOIUrl":"https://doi.org/10.1002/adhm.202404296","url":null,"abstract":"Significant developments have been made in the field of wearable healthcare by utilizing soft materials for the construction of electronic sensors. However, the lack of adaptability to complex topologies, such as ear canal, results in inadequate sensing performance. Here, we report an in-ear physiological sensor with mechanical adaptability, which softens upon contact with the ear canal's skin, thus reducing the sensor-skin mechanical mismatch and interface impedance. An efficient strategy of mechanical adjustment and switching is exploited to increase the softness of the device, leading to a significant decrease in Young's modulus from 30.5 MPa of thermoplastic polyurethane (TPU) to 0.86 MPa of TPU/Ecoflex foam (TEF).The mechanical adaptability at body temperature endows the in-ear device improved device-canal contact area and interface stability. As a result, the TEF-based in-ear device demonstrates reliable sensing, low motion artifact, and high comfort in electroencephalography (EEG) and core body temperature sensing. High quality EEG signals of alpha, beta, delta, and gamma are measured during different activities. Moreover, the TEF-based in-ear device exhibits high reusability for over 4 months, which makes it suitable for long-term healthcare monitoring.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404296"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanofiber-based Multifunctional Microspheres for Rapid Hemostasis and Microorganism Removal of Water.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202403679

Qing Li, Lingjun Ye, Yanqiu Leng, Kun Yu, Enling Hu, Fei Lu, Ruiqi Xie, Sha Jiang, Hang Gao, Rong Bao, Fangyin Dai, Guangqian Lan

{"title":"Nanofiber-based Multifunctional Microspheres for Rapid Hemostasis and Microorganism Removal of Water.","authors":"Qing Li, Lingjun Ye, Yanqiu Leng, Kun Yu, Enling Hu, Fei Lu, Ruiqi Xie, Sha Jiang, Hang Gao, Rong Bao, Fangyin Dai, Guangqian Lan","doi":"10.1002/adhm.202403679","DOIUrl":"https://doi.org/10.1002/adhm.202403679","url":null,"abstract":"Constructing hemostats capable of effectively controlling severe hemorrhage from irregular wounds presents significant challenges and imperatives. In this study, a novel approach is introduced using nanofibrous chitin microspheres (NCM) that are compressed to 60% strain (NCM-60%) to amplify their water-initiated expansion performance. This unique capacity allows NCM-60% to efficiently conform to and fill irregular bleeding cavities, even those of varying depths and curvatures, thereby promoting rapid blood coagulation at deep hemorrhage sites. NCM-60% exhibits effective control of severe femoral artery and \"J\"-shaped liver hemorrhages in 151 ± 6 s and 68 ± 15 s, respectively, revealing its exceptional hemostatic efficacy. Furthermore, NCM-60% exhibited promising capabilities in removing microbes from water, achieving removal rates of over 96% of bacteria. Blood compatibility assessments and cytotoxicity tests further confirmed the favorable biocompatibility of NCM-60%. Importantly, NCM-60% is found to biodegrade and be absorbed in vivo within 12 weeks. This study represents the first instance of leveraging chitin nanofiber-based biomaterials to design water-initiated expansion micro-hemostat, and integrate hemostatic functions with waterborne microorganism removal, thereby expanding the potential applications of micro-nanostructural materials in emergency first-aid scenarios.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403679"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Multisynergistic Strategy for Bone Tumor Treatment: Orchestrating Oxidative Stress and Autophagic Flux Inhibition by Environmental-Response Nanoparticle.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202402872

Xiaochen Chen, Pengfei Tian, Wenwen Chai, Liyan Zhang, Muyan Qin, Mengke Fan, Na Liang, Jua Kim, Yansong Wang, Weijia William Lu, Deping Wang, Xu Cui, Haobo Pan

{"title":"A Multisynergistic Strategy for Bone Tumor Treatment: Orchestrating Oxidative Stress and Autophagic Flux Inhibition by Environmental-Response Nanoparticle.","authors":"Xiaochen Chen, Pengfei Tian, Wenwen Chai, Liyan Zhang, Muyan Qin, Mengke Fan, Na Liang, Jua Kim, Yansong Wang, Weijia William Lu, Deping Wang, Xu Cui, Haobo Pan","doi":"10.1002/adhm.202402872","DOIUrl":"https://doi.org/10.1002/adhm.202402872","url":null,"abstract":"Tumor therapy has advanced significantly in recent years, but tumor cells can still evade and survive the treatment through various mechanisms. Notably, tumor cells use autophagy to sustain viability by removing impaired mitochondria and clearing excess reactive oxygen species (ROS). In this study, the aim is to amplify intracellular oxidative stress by inhibiting mitochondrial autophagic flux. Multisynergistic environmental-response nanoparticles (ERNs) are engineered by integrating gold nanoparticles and copper peroxide with borosilicate bioactive glass. The controlled release of copper and inhibition of autophagy flux triggered an overabundance and accumulation of oxidative stress within the tumor cells. This stress triggered immunogenic tumor cell death, believed to initiate a systemic immune response. The tumor microenvironment (TME) transitioned back to a normal physiological state as tumor cells are ablated. ERNs responded to the microenvironment changes by depositing hydroxyapatite on the surface and spontaneously enhancing bone regeneration. This innovative formulation facilitates the functional transition of ERNs from \"anti-tumor therapy\" to \"biomineralization\" that kills cancers and induces new bone formation. Overall, it is shown that the ERNs effectively eradicate cancers by utilizing chemodynamic therapy, starvation therapy, and immunotherapy.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402872"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Target-Attacking Tumor Cells and M2 Macrophages via a Triple-Responsive Nanoassembly for Complete Metastasis Blocking.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202304096

Bei Wang, Hao Cheng, Zhongsheng Ji, Zijun Jiang, Rong Wang, Yang Ding, Jiang Ni

{"title":"Synergistic Target-Attacking Tumor Cells and M2 Macrophages via a Triple-Responsive Nanoassembly for Complete Metastasis Blocking.","authors":"Bei Wang, Hao Cheng, Zhongsheng Ji, Zijun Jiang, Rong Wang, Yang Ding, Jiang Ni","doi":"10.1002/adhm.202304096","DOIUrl":"https://doi.org/10.1002/adhm.202304096","url":null,"abstract":"Collaboration of cancerous cells and microenvironment is the root for tumor spreading, leading to difficulty in complete metastasis blockage via mono-intervention. Herein, a triple-responsive nanoassembly is designed for orienting tumor cells and migration-driving M2 tumor associated macrophages (TAMs) in microenvironment for efficient anti-metastatic therapy. Structurally, a reactive oxygen species (ROS)-responsive crosslinked short-chain polyquaternium is synthesized to bridge graphene oxide (GO) scaffold with apolipoprotein A-I crown via borate-crosslinking, electrostatic adherence, and coordinative coupling. The protein-crowning polymeric GO nanoparticles could give multimodal shielding and triple-responsive release of doxorubicin and Snail-targeted siRNA. Tailor-made apolipoprotein A-I crown fulfills nanoparticles synergistically attacking tumor cells and M2 TAMs via binding with overexpressed scavenger receptors. The findings witness the targeted accumulation and potent cytotoxicity of the hybrid nanoparticles for M2 TAMs and tumor cells; especially, elimination of M2 TAMs in tumor microenvironment holds back Snail-enhancing transforming growth factor (TGF)-β signal pathway, which collaborates with Snail silencing in tumor cells to reverse epithelial mesenchymal transition (EMT) and metastasis-promoting niche. Collectively, the synergistic targeting therapeutic platform could provide a promising solution for metastatic tumor treatment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2304096"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific gFET-Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health-Relevant Bacterium Roseburia Intestinalis.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202403827

Yiting Zhang, Hu Xing, Runliu Li, Jakob Andersson, Anil Bozdogan, Robert Strassl, Bastian Draphoen, Mika Lindén, Marius Henkel, Uwe Knippschild, Roger Hasler, Christoph Kleber, Wolfgang Knoll, Ann-Kathrin Kissmann, Frank Rosenau

{"title":"Specific gFET-Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health-Relevant Bacterium Roseburia Intestinalis.","authors":"Yiting Zhang, Hu Xing, Runliu Li, Jakob Andersson, Anil Bozdogan, Robert Strassl, Bastian Draphoen, Mika Lindén, Marius Henkel, Uwe Knippschild, Roger Hasler, Christoph Kleber, Wolfgang Knoll, Ann-Kathrin Kissmann, Frank Rosenau","doi":"10.1002/adhm.202403827","DOIUrl":"https://doi.org/10.1002/adhm.202403827","url":null,"abstract":"Roseburia intestinalis, enriched in the gut, is closely associated with obesity, intestinal inflammation, and other diseases. A novel detection method for R. intestinalis to replace the commonly used 16S rRNA sequencing technique is aim to developed, thus enabling real-time and low-cost monitoring of gut microbiota. The optimal solution is to utilize rGO-FET (reduced graphene oxide field-effect transistor) functionalized with aptamers. Due to the high sensitivity of graphene sensors to electronic changes in the system, it is anticipated to achieve detection sensitivity that traditional fluorescence detection techniques cannot attain. The previous work reported a nucleic acid aptamer library, Ri 7_2, capable of quantitatively tracking R. intestinalis in complex systems. However, due to the complexity of the aptamer library itself, large-scale industrial synthesis is challenging, significantly limiting its further commercial application potential. Therefore, in this study, through Next-Generation Sequencing analysis, four representative single aptamers from the aptamer library is strategically selected, named A-Rose 1, A-Rose 2, A-Rose 3, and A-Rose 4, and confirmed their excellent performance similar to the aptamer library Ri 7_2. Furthermore, aptamer-modified rGO-FET demonstrated universality in detecting R. intestinalis in a series of biochemical analyses, providing a novel and powerful diagnostic tool for the clinical diagnosis of R. intestinalis.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403827"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiscale Thermal Analysis of Gold Nanostars in 3D Tumor Spheroids: Integrating Cellular-Level Photothermal Effects and Nanothermometry via X-Ray Spectroscopy.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-11 DOI: 10.1002/adhm.202403799

Rosalía López-Méndez, Anastasiia Dubrova, Javier Reguera, Rául Magro, Fátima Esteban-Betegón, Ana Parente, Miguel Ángel García, Julio Camarero, Emiliano Fonda, Claire Wilhelm, Álvaro Muñoz-Noval, Ana Espinosa

{"title":"Multiscale Thermal Analysis of Gold Nanostars in 3D Tumor Spheroids: Integrating Cellular-Level Photothermal Effects and Nanothermometry via X-Ray Spectroscopy.","authors":"Rosalía López-Méndez, Anastasiia Dubrova, Javier Reguera, Rául Magro, Fátima Esteban-Betegón, Ana Parente, Miguel Ángel García, Julio Camarero, Emiliano Fonda, Claire Wilhelm, Álvaro Muñoz-Noval, Ana Espinosa","doi":"10.1002/adhm.202403799","DOIUrl":"https://doi.org/10.1002/adhm.202403799","url":null,"abstract":"In the pursuit of enhancing cancer treatment efficacy while minimizing side effects, near-infrared (NIR) photothermal therapy (PTT) has emerged as a promising approach. By using photothermally active nanomaterials, PTT enables localized hyperthermia, effectively eliminating cancer cells with minimal invasiveness and toxicity. Among these nanomaterials, gold nanostars (AuNS) stand out due to their tunable plasmon resonance and efficient light absorption. This study addresses the challenge of measuring nanoscale temperatures during AuNS-mediated PTT by employing X-ray absorption spectroscopy (XAS) within 3D tumor spheroids. It also aims to investigate the heat generated at the nanoscale and the resultant biological damage observed at a larger scale, utilizing confocal microscopy to establish connections between AuNS heat generation, tissue damage, and their impacts on cellular structure. These nanoscale and microscale thermal effects have been compared with macroscopic values obtained from infrared thermography, as part of a multiscale thermal analysis. The findings underscore the efficacy of AuNS in enhancing PTT and provide insights into the spatial distribution of thermal effects within tumor tissues. This research advances the understanding of localized hyperthermia in cancer therapy and underscores the potential of AuNS-based PTT for clinical applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403799"},"PeriodicalIF":10.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curvature Perception of Mesenchymal Cells on Mesoscale Topographies.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-10 DOI: 10.1002/adhm.202402865

Kerstin Frey, Michael Brunner, Christóbal Curio, Ralf Kemkemer

{"title":"Curvature Perception of Mesenchymal Cells on Mesoscale Topographies.","authors":"Kerstin Frey, Michael Brunner, Christóbal Curio, Ralf Kemkemer","doi":"10.1002/adhm.202402865","DOIUrl":"https://doi.org/10.1002/adhm.202402865","url":null,"abstract":"Cells can sense geometrical cues with sizes of several tens of micrometers in their vicinity. Recent in vitro studies show that cells can adapt their shape, align along specific directions, or regulate other cellular functions when grown on surfaces with curvatures larger than their size. Although possible mechanisms for such responses like the alignment along axial cues have been suggested, a detailed understanding of the involved cellular processes remains open. This work addresses this gap by systematically investigating mesenchymal cell and nucleus orientation responses using a low-cost model surface platform, the CurvChip. Using an array of cylindrically curved topographies with radii of curvatures ranging from tens to hundreds of micrometers, the contact guidance response of cells and nuclei is quantified in dependence on substratum curvature and manipulation of cytoskeletal components. Results suggest a desired perceived curvature for the investigated cells, and a very sensitive and robust curvature perception mechanism, as the effect of pharmacological manipulation of cytoskeletal components is relatively small. Furthermore, a comparison with previously published work strengthens the hypothesis of an involvement of the nucleus in the cell response to three-dimensional (3D) curvatures.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402865"},"PeriodicalIF":10.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melt Electrowriting of Elastic Scaffolds Using PEOT-PBT Multi-block Copolymer.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-10 DOI: 10.1002/adhm.202402914

Armin Amirsadeghi, Pavan Kumar Reddy Gudeti, Sietse Tock, Marcus Koch, Daniele Parisi, Marleen Kamperman, Małgorzata Katarzyna Włodarczyk-Biegun

{"title":"Melt Electrowriting of Elastic Scaffolds Using PEOT-PBT Multi-block Copolymer.","authors":"Armin Amirsadeghi, Pavan Kumar Reddy Gudeti, Sietse Tock, Marcus Koch, Daniele Parisi, Marleen Kamperman, Małgorzata Katarzyna Włodarczyk-Biegun","doi":"10.1002/adhm.202402914","DOIUrl":"https://doi.org/10.1002/adhm.202402914","url":null,"abstract":"Melt electrowriting (MEW) is a powerful additive manufacturing technique to produce tissue engineering scaffolds. Despite its strength, it is limited by a small number of processable polymers. Therefore, to broaden the library of materials for MEW, we investigated the printability of poly(ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT-PBT), a thermoplastic elastomer. The effect of different printing parameters and material thermal degradation are studied. It is observed that the material is stable for >60 min at a printing temperature of 195 °C in a nitrogen environment. Next, two types of designs are printed and characterized: mesh-like and semi-random scaffolds. For both types of designs, PEOT-PBT scaffolds reveal a higher yield strain, and lower Young's modulus as compared to control polycaprolactone scaffolds. Biological studies performed using mouse embryonic fibroblasts (NIH-3T3) show good cell viability and metabolic activity on all print scaffolds. SEM imaging reveals actively migrating cells on PEOT-PBT mesh scaffolds after 24 h of culture and 98.87% of pore bridging by cells after 28 days of culture. Immunofluorescence staining shows decreased expression of alpha-smooth muscle actin from day 14 to day 28 in PEOT-PBT mesh scaffolds. Overall, it is shown that melt electrowritten PEOT-PBT scaffolds have great potential for soft tissue regeneration.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402914"},"PeriodicalIF":10.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Charge Engineering of Star-Shaped Organic Photosensitizers Enables Efficient Type-I Radicals for Photodynamic Therapy of Multidrug-Resistant Bacterial Infection.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-12-08 DOI: 10.1002/adhm.202402615

Jie Yu, Wenchang Xu, Huan Chen, Haitao Yuan, Yu Wang, Xiandie Qian, Jie Zhang, Yu Ji, Qi Zhao, Shengliang Li

{"title":"Charge Engineering of Star-Shaped Organic Photosensitizers Enables Efficient Type-I Radicals for Photodynamic Therapy of Multidrug-Resistant Bacterial Infection.","authors":"Jie Yu, Wenchang Xu, Huan Chen, Haitao Yuan, Yu Wang, Xiandie Qian, Jie Zhang, Yu Ji, Qi Zhao, Shengliang Li","doi":"10.1002/adhm.202402615","DOIUrl":"https://doi.org/10.1002/adhm.202402615","url":null,"abstract":"Infection induced by multidrug-resistant bacteria is now the second most common cause of accidental death worldwide. However, identifying a high-performance strategy with good efficiency and low toxicity is still urgently needed. Antibacterial photodynamic therapy (PDT) is considered a non-invasive and efficient approach with minimal drug resistance. Whereas, the precise molecular design for highly efficient oxygen-independent type-I photosensitizers is still undefined. In this work, the regulation of the positive charge of star-shaped NIR-emissive organic photosensitizers can boost radical generation for the efficient treatment of wounds infected with multidrug-resistant bacteria. With positive charge engineering, TPAT-DNN, which has six positive charges, mainly produces hydroxyl radicals via the type-I pathway, while TPAT-DN, which has three positive charges, tends to generate singlet oxygen and superoxide radicals. For multidrug-resistant bacteria, TPAT-DNN exhibited specific killing effects on multidrug-resistant gram-positive bacteria at low concentrations, while TPAT-DN is similar antibacterial effects on both multidrug-resistant gram-negative and gram-positive bacteria. Furthermore, the efficiency and safety of TPAT-DNN for eradicating multidrug-resistant bacteria methicillin-resistant S. aureus (MRSA) infection and accelerating wound healing in an MRSA-infected mouse model are demonstrated. This work offers a new approach toward manipulating efficient type-I photosensitizers for MRSA treatment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402615"},"PeriodicalIF":10.0,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0