Advanced Healthcare Materials最新文献_第10页

Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin-Activated Platelet-Rich Plasma Self-Assembly.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-29 DOI: 10.1002/adhm.202404295

Xiaotong Shi, Zongliang Wang, Zhiguo Bi, Jianguo Liu, Peibiao Zhang

{"title":"Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin-Activated Platelet-Rich Plasma Self-Assembly.","authors":"Xiaotong Shi, Zongliang Wang, Zhiguo Bi, Jianguo Liu, Peibiao Zhang","doi":"10.1002/adhm.202404295","DOIUrl":"https://doi.org/10.1002/adhm.202404295","url":null,"abstract":"Platelet-rich plasma (PRP) is characterized by elevated concentrations of growth factors that facilitate bone repair. Nonetheless, the effective integration of PRP with bone implants and the sustained release of its active constituents pose significant challenges. In this study, thrombin is grafted onto the surface of polyetheretherketone (PEEK) via an N,N'-Disuccinimidyl Carbonate (DSC) linker and the retained enzymatic activity of thrombin enables the controlled activation of PRP self-assembly, resulting in the formation of a functional bio-gel layer. The optimal thrombin concentration to be 100 U/ mL-1 is determined, at which point both the grafting amount and enzymatic activity of thrombin reaches their peak, with no further increases observed at higher concentrations. PRP solutions with varying platelet enrichment ratios are subsequently activated on the thrombin-grafted PEEK surface, yielding self-assembled bio-gels capable of sustained growth factor release for up to 16 days. The thrombin-activated PRP bio-gel on PEEK surface not only enhances in vitro cell adhesion, proliferation, osteogenic differentiation, vascularization and specific polarization of macrophages, but also effectively facilitates in vivo angiogenesis, immunomodulation and bone formation in a platelet dose-dependent manner. Consequently, the thrombin-activated PRP gel presents a promising strategy for the biological functionalization of PEEK implants in orthopedic applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404295"},"PeriodicalIF":10.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Solvatochromic and Photosensitized Lipid Droplet Probe Detects Local Polarity Heterogeneity and Labels Interacting Proteins in Human Liver Disease Tissue.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-28 DOI: 10.1002/adhm.202404713

Yuhui Wang, Hengke Guo, Wang Wan, Biao Jing, Yulong Bai, Jialu Sun, Xin Zhang, Zhenming Gao, Yu Liu, Xuepeng Dong

{"title":"A Solvatochromic and Photosensitized Lipid Droplet Probe Detects Local Polarity Heterogeneity and Labels Interacting Proteins in Human Liver Disease Tissue.","authors":"Yuhui Wang, Hengke Guo, Wang Wan, Biao Jing, Yulong Bai, Jialu Sun, Xin Zhang, Zhenming Gao, Yu Liu, Xuepeng Dong","doi":"10.1002/adhm.202404713","DOIUrl":"https://doi.org/10.1002/adhm.202404713","url":null,"abstract":"The intricate morphology, physicochemical properties, and interacting proteins of lipid droplets (LDs) are associated with cell metabolism and related diseases. To uncover these layers of information, a solvatochromic and photosensitized LDs-targeted probe based on the furan-based D-D-π-A scaffold is developed to offer the following integrated functions. First, the turn-on fluorescence of the probe upon selectively binding to LDs allows for direct visualization of their location and morphology. Second, its solvatochromic fluorescence with linear correlation to polarity quantifies micro-environmental heterogeneity among LDs. Third, the unique photosensitized properties enable photocatalytic proximity labeling and enrichment of LDs-interacting proteins, ready for potential downstream proteomic analysis. These functions are exemplified using artificial LDs in buffer, stressed liver cell line, and diseased liver tissues biopsied from patients. While most LD sensors only offer fluorescence imaging functions, the multi-functional LD probe reported herein integrates both singlet fluorescence and triplet photosensitization properties for LDs studies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404713"},"PeriodicalIF":10.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Cell Membrane-Coated Keratin Nanoparticles Attenuated Intervertebral Disc Degeneration by Remodeling the Disc Microenvironment.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-28 DOI: 10.1002/adhm.202404173

Hongyuan Xing, Run Li, Zizhan Huang, Zhongyang Gao, Qijiang Mao, Yifan Shen, Guanrui Huang, Guangyu Chu, Yue Wang

{"title":"Engineered Cell Membrane-Coated Keratin Nanoparticles Attenuated Intervertebral Disc Degeneration by Remodeling the Disc Microenvironment.","authors":"Hongyuan Xing, Run Li, Zizhan Huang, Zhongyang Gao, Qijiang Mao, Yifan Shen, Guanrui Huang, Guangyu Chu, Yue Wang","doi":"10.1002/adhm.202404173","DOIUrl":"https://doi.org/10.1002/adhm.202404173","url":null,"abstract":"Characterized by a cascade of profound changes in nucleus pulposus (NP) cells, extracellular matrix (ECM), and biomechanics, intervertebral disc degeneration is a common multifactorial condition that may lead to various degenerative lumbar disorders. Therapeutic strategies targeting a single factor have shown limited efficacy in treating disc degeneration, and approaches that address multiple pathological ingredients are barely reported. In this study, engineered cell membrane-encapsulated keratin nanoparticles are developed to simultaneously alleviate NP cell senescence and promote ECM remodeling. To achieve this, salivary acid glycoengineered adipose mesenchymal stem cell membranes are used to coat keratin, a core protein for structural support and cellular protection. The synthesized cell membrane-coated keratin nanoparticles (MKNs) effectively protected mitochondrial integrity in NP cells from oxidative stress-induced damage. Moreover, MKNs modulate mitochondrial metabolism and attenuate NP cell senescence. In addition, MKNs activate integrins at the cell membrane and enhance the interactions between NP cells and ECM, resulting in increased ECM anabolism and decreased catabolism. The proposed multi-targeted strategy to block the degenerative cycle inside the disc is efficacious for treating disc degeneration and may have the potential for clinical application.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404173"},"PeriodicalIF":10.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluoropolymer-Single Crystal Nanocomposite Based Transducer Fabrication for Bio-Imaging.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-28 DOI: 10.1002/adhm.202403711

Nagendra Singh, S K Biswas

{"title":"Fluoropolymer-Single Crystal Nanocomposite Based Transducer Fabrication for Bio-Imaging.","authors":"Nagendra Singh, S K Biswas","doi":"10.1002/adhm.202403711","DOIUrl":"https://doi.org/10.1002/adhm.202403711","url":null,"abstract":"Fluoropolymer alone, as an alternative to lead-based piezoelectric materials, has shown multiple challenges to develop useful sensors for solving real-world problems such as photoacoustic, ultrasound pulse echo, and other non-destructive testing. This work demonstrates the fabrication of high frequency and wide bandwidth transducers with fluoropolymer and highly polarizing cubic single crystal Barium titanate (BaTiO3) ceramic composite for high resolution in-vivo photo-acoustic and ultrasound imaging. For transducer fabrication, a customized bio-compatible nanocomposite sensor film of PVDF-TrFE (Polyvinylidene fluoride trifluoroethylene)/BaTiO3 (BTO) is synthesized by drop and dry in heating-cum-electro-poling system for advancing polarization, crystallinity, and higher charge generation. The ratio of nanofiller cubic single crystal BTO and PVDF-TrFE is optimized using characterization techniques such as FTIR, XRD and electrometer. Thereafter, SEM and TGA analyses are performed to study the surface morphology and thermal stability of the sensing film. Transducers with central frequencies varying from 17 to 42MHz are fabricated and tested for both pulse-echo mode and receiving photoacoustic signals. These transducers are used for sensing photoacoustic signals generated from hemoglobin and eumelanin and further for ultrasound and photoacoustic imaging. The imaging results are compared with the results obtained using a commercial ultrasound and photoacoustic imaging device. To the best of the knowledge and available literature, for the first time, the fabrication of ultrasound/photoacoustic transducers with cubic single-crystal nanofiller and fluoropolymer nanocomposite is showed. The detailed transducer fabrication method, characterization results, and imaging of biological tissue using photoacoustic and ultrasound are presented.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403711"},"PeriodicalIF":10.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From X- To J-Aggregation: Subtly Managing Intermolecular Interactions for Superior Phototheranostics with Precise 1064 nm Excitation.

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202404322

Jun Tang, Leilei Si, Yigang Wang, Guomin Xia, Hongming Wang

{"title":"From X- To J-Aggregation: Subtly Managing Intermolecular Interactions for Superior Phototheranostics with Precise 1064 nm Excitation.","authors":"Jun Tang, Leilei Si, Yigang Wang, Guomin Xia, Hongming Wang","doi":"10.1002/adhm.202404322","DOIUrl":"https://doi.org/10.1002/adhm.202404322","url":null,"abstract":"The stacking mode in aggregate state results from a delicate balance of supramolecular interactions, which closely affects the optoelectronic properties of organic π-conjugated systems. Then, managing these interactions is crucial for advancing phototheranostics, yet remains challenging. A subtle strategy involving peripheral phenyl groups is debuted herein to transform X-aggregated SQ-H into J-aggregated SQ-Ph, reorienting intermolecular dipole interactions while rationally modulating π-π interactions. Co-assembled with liposomes (DSPE-PEG2000), SQ-Ph nanoparticles (NPs) exhibit low toxicity, superior biocompatibility, and a bathochromic shift to the 1064 nm match-excited NIR-II region, with a fluorescence brightness (ε1064 nm ΦNIR-II) of 4129 M-1 cm-1 and a photothermal conversion efficiency (PCE) of 48.3%. Preliminary in vivo experiments demonstrate that SQ-Ph NPs achieve a signal-to-background ratio (SBR) of up to 14.29 in NIR-II fluorescence imaging (FLI), enabling highly efficient photothermal therapy (PTT) of tumors guided by combined photoacoustic imaging (PAI). This study not only enriches the J-aggregation library but also provides a paradigm for optimizing photosensitizers at the supramolecular level.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404322"},"PeriodicalIF":10.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Adv. Healthcare Mater. 3/2025

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202570016

引用次数: 0

Bilayer Scaffolds Synergize Immunomodulation and Rejuvenation via Layer-Specific Release of CK2.1 and the “Exercise Hormone” Lac-Phe for Enhanced Osteochondral Regeneration (Adv. Healthcare Mater. 3/2025)

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202570015

Po-Lin Liu, Shu-Hang He, Zhi-Han Shen, Xu-Ran Li, Qing-Song Deng, Zhan-Ying Wei, Chang-Ru Zhang, Xiao-Qiu Dou, Tong-He Zhu, Helen Dawes, Jian Lu, Shang-Chun Guo, Shi-Cong Tao

{"title":"Bilayer Scaffolds Synergize Immunomodulation and Rejuvenation via Layer-Specific Release of CK2.1 and the “Exercise Hormone” Lac-Phe for Enhanced Osteochondral Regeneration (Adv. Healthcare Mater. 3/2025)","authors":"Po-Lin Liu, Shu-Hang He, Zhi-Han Shen, Xu-Ran Li, Qing-Song Deng, Zhan-Ying Wei, Chang-Ru Zhang, Xiao-Qiu Dou, Tong-He Zhu, Helen Dawes, Jian Lu, Shang-Chun Guo, Shi-Cong Tao","doi":"10.1002/adhm.202570015","DOIUrl":"https://doi.org/10.1002/adhm.202570015","url":null,"abstract":"Osteochondral RecoveryThe person practicing Tai Chi in the image symbolizes the developed biphasic scaffold. Surrounding the person are two water droplets resembling the “yin” and “yang” of Tai Chi, symbolizing the simultaneous repair of bone and cartilage. Embedded within the yin and yang are the bioactive molecules CK2.1 and “exercise hormone” Lac-Phe, indicating that these molecules maintain bone-cartilage repair. The water and fish in the small pond represent various repair-promoting factors in the osteochondral microenvironment, while the background symbolizes a healthy ecological environment, representing a favorable regenerative microenvironment around the bone and cartilage. The overall meaning is that CK2.1 and “exercise hormone” Lac-Phe, through the scaffold as a medium, regulate the regenerative microenvironment, mobilizing various factors beneficial for repair to achieve osteochondral regeneration. More details can be found in the article 2402329 by Shang-Chun Guo, Shi-Cong Tao, and co-workers. Image credit: CYANTIFICA Lina Cao.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"14 3","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202570015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content (Adv. Healthcare Mater. 3/2025)

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202570018

Kim Truc Nguyen, Xilal Y. Rima, Luong T. H. Nguyen, Xinyu Wang, Kwang Joo Kwak, Min Jin Yoon, Hong Li, Chi-Ling Chiang, Jacob Doon-Ralls, Kelsey Scherler, Shannon Fallen, Stephanie L. Godfrey, Julie A. Wallick, Setty M. Magaña, Andre F. Palmer, Inyoul Lee, Christopher C. Nunn, Kimberly M. Reeves, Henry G. Kaplan, Jason D. Goldman, James R. Heath, Kai Wang, Preeti Pancholi, L. James Lee, Eduardo Reátegui

{"title":"Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content (Adv. Healthcare Mater. 3/2025)","authors":"Kim Truc Nguyen, Xilal Y. Rima, Luong T. H. Nguyen, Xinyu Wang, Kwang Joo Kwak, Min Jin Yoon, Hong Li, Chi-Ling Chiang, Jacob Doon-Ralls, Kelsey Scherler, Shannon Fallen, Stephanie L. Godfrey, Julie A. Wallick, Setty M. Magaña, Andre F. Palmer, Inyoul Lee, Christopher C. Nunn, Kimberly M. Reeves, Henry G. Kaplan, Jason D. Goldman, James R. Heath, Kai Wang, Preeti Pancholi, L. James Lee, Eduardo Reátegui","doi":"10.1002/adhm.202570018","DOIUrl":"https://doi.org/10.1002/adhm.202570018","url":null,"abstract":"Biochip Antigen and RNA AssayThe Biochip Antigen and RNA Assay (BARA) enables the simultaneous detection of nucleic acids and antigens by integrating immunofluorescence and fluorescent in situ hybridization at a single-particle resolution. The BARA thus enables the highly sensitive quantification of virions in complex biofluids and continued monitoring of viral content in extracellular vesicles. Overall, the BARA offers an unmatched perspective into virion-mediated outbreaks. More details can be found in article 2400622 by Eduardo Reátegui and co-workers.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":"14 3","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202570018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinstructive Liquefied Pockets in Hierarchical Hydrogels and Bioinks (Adv. Healthcare Mater. 3/2025)

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202570017

Maryam Ghasemzadeh-Hasankolaei, Tiago R. Correia, João F. Mano

引用次数: 0

Enhancement of Bone Repair in Diabetic Rats with Metformin-Modified Silicified Collagen Scaffolds (Adv. Healthcare Mater. 3/2025) 二甲双胍改性硅化胶原支架促进糖尿病大鼠的骨修复（Adv. Healthcare Mater.）

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-27 DOI: 10.1002/adhm.202570019

Yi-Rong Wang, Xiao-Xia Zhang, Xu-Xu Chen, Xin-Hua Yin, Ming Yang, Kuo Jiang, Shi-Chang Liu

引用次数: 0