Advanced Healthcare Materials最新文献_第10页

Hierarchically Structured Microparticles Encapsulated With Multiple Plant-Derived Bioactives for Psoriasis Therapy. 含有多种植物源性生物活性的分层结构微颗粒用于银屑病治疗。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-07 DOI: 10.1002/adhm.71803

Dingyuan Chen, Minhui Lu, Yuanjin Zhao

{"title":"Hierarchically Structured Microparticles Encapsulated With Multiple Plant-Derived Bioactives for Psoriasis Therapy.","authors":"Dingyuan Chen, Minhui Lu, Yuanjin Zhao","doi":"10.1002/adhm.71803","DOIUrl":"https://doi.org/10.1002/adhm.71803","url":null,"abstract":"Traditional Chinese medicine bioactives have considerable therapeutic value in psoriasis management, while their clinical applications are still constrained by the insufficient therapeutic efficacy of single components, uncontrolled release, and low local bioavailability. In this study, we report a hierarchically structured microparticle system loaded with multiple plant-derived bioactives for psoriasis therapy. The microparticles of sodium alginate (SA) hydrogel with polydopamine (PDA)-modification were fabricated through microfluidics, co-loaded with paeoniflorin (PF) and ginger-derived exosome-like nanoparticles (GELNs). Benefiting from the porous structure of hydrogel and adhesive property of PDA, the microparticles enable stable co-encapsulation of multiple plant-derived bioactives and achieve sustained release at lesion sites, enhancing local bioavailability. The encapsulated PF and GELNs exert complementary anti-inflammatory, antioxidant, and anti-proliferative effects, collectively regulating key pathological processes associated with psoriasis. These results prove that the microparticle-based multiple plant-derived bioactives delivery system provides a safe, cost-effective, and translatable approach for long-term topical treatment of psoriasis, highlighting their therapeutic potential in inflammatory skin diseases.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71803"},"PeriodicalIF":11.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rotor-Controlled Vibronic Coupling Enables Efficient Near-Infrared Phosphorescence in Iridium(III) Complexes for Enhanced Mitochondrial Photodynamic Therapy. 转子控制的振动耦合使铱（III）配合物中有效的近红外磷光用于增强线粒体光动力治疗。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-07 DOI: 10.1002/adhm.71805

Wei He, Tianyu Li, Jianyu Zhang, Ziyu Cui, Gian Albert Alfani, Jiaqi Peng, Faizal Khan, Zheng Zhao, Zijie Qiu, Jianwei Sun, Jacky Wing Yip Lam, Ryan Tsz Kin Kwok, Parvej Alam, Ben Zhong Tang

{"title":"Rotor-Controlled Vibronic Coupling Enables Efficient Near-Infrared Phosphorescence in Iridium(III) Complexes for Enhanced Mitochondrial Photodynamic Therapy.","authors":"Wei He, Tianyu Li, Jianyu Zhang, Ziyu Cui, Gian Albert Alfani, Jiaqi Peng, Faizal Khan, Zheng Zhao, Zijie Qiu, Jianwei Sun, Jacky Wing Yip Lam, Ryan Tsz Kin Kwok, Parvej Alam, Ben Zhong Tang","doi":"10.1002/adhm.71805","DOIUrl":"https://doi.org/10.1002/adhm.71805","url":null,"abstract":"Near-infrared (NIR) phosphorescent iridium(III) complexes hold immense promise for biomedical imaging and optoelectronics but suffer from poor quantum yields due to the energy gap law, which amplifies nonradiative decay through vibrational quenching at lower energies. We introduce a molecular rotor engineering strategy that systematically modulates excited-state dynamics by varying the placement of the rotor on chromophoric and ancillary ligands. Restricting rotational motion upon aggregation or in rigid PMMA matrices dramatically suppresses nonradiative pathways, boosting quantum yields from ∼4% in solution to 65% in 10 wt% PMMA films, with further enhancements in the solid state (12.5%) and aggregates (14.5%). Structural and computational analyses reveal that molecular rotors govern excited-state behavior through configuration interaction between metal-to-ligand charge transfer (3MLCT) and ligand-centered (3LC) states, enabling dynamic population redistribution that tunes radiative and nonradiative channels without requiring discrete state conversion. This aggregation-induced phosphorescence (AIP) mechanism circumvents traditional energy gap limitations. The resulting efficient NIR emission demonstrates selective mitochondrial targeting and potent photodynamic anticancer activity, with aggregation-enhanced quantum yields proving essential for effective 1O2 generation. This rotor engineering approach establishes a versatile design framework for high-performance NIR emitters across therapeutic, imaging, and optoelectronic applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71805"},"PeriodicalIF":11.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vat-Polymerized 3D Printed Multichannel Nerve Conduit Functionalized With Bacopa monnieri and Collagen Dipeptides Encased in a Bilayer Scaffold for Peripheral Nerve Regeneration. 用双层支架包裹假马齿草和胶原二肽功能化的3D打印多通道神经导管用于周围神经再生。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71791

Arabinda Majhi, Shruti Mandal, Sachin Latiyan, Kaushik Chatterjee, Sheenam Sharma, Rishabh Chaudhary, Sumeet Gupta, Partha Roy, Debrupa Lahiri

{"title":"Vat-Polymerized 3D Printed Multichannel Nerve Conduit Functionalized With Bacopa monnieri and Collagen Dipeptides Encased in a Bilayer Scaffold for Peripheral Nerve Regeneration.","authors":"Arabinda Majhi, Shruti Mandal, Sachin Latiyan, Kaushik Chatterjee, Sheenam Sharma, Rishabh Chaudhary, Sumeet Gupta, Partha Roy, Debrupa Lahiri","doi":"10.1002/adhm.71791","DOIUrl":"https://doi.org/10.1002/adhm.71791","url":null,"abstract":"Peripheral nerve injury (PNI) affects both autonomic and somatic signaling. PNI autografts are limited by donor nerve morbidity and availability. The intricate structure of peripheral nerves cannot be accurately reproduced using approved nerve conduits. They lack neuroprotective and antioxidant properties that could aid nerve regeneration and reduce reactive oxygen species (ROS) after injury. This study presents a multichannel hydrogel nerve conduit that incorporates Bacopa monnieri extract for its antioxidant and neuroprotective properties, along with collagen dipeptides. The design incorporates nanofibrous electrospun and porous hydrogel matrices that replicate the neuronal extracellular matrix. In vitro, hydrogels reduce oxidative stress and increase nerve cell adhesion and proliferation. In vivo study encapsulates the multichannel nerve conduit within a bilayer scaffold of electrospun nanofibers and a 3D-printed polymeric framework. The 3D-printed framework provides mechanical stability and reduces hydrogel degradation, while the electrospun layer covers the epineurium for long-term nerve regeneration. A partially transacted sciatic nerve model reveals axonal development, myelination, and improvement in motor-sensory function, suggesting nerve regeneration. The additive therapeutic effect of Bacopa monnieri and collagen dipeptides increased the diameter of myelinated nerve fibers, enhanced nerve saltatory conduction, and promoted fascicular development. This multichannel nerve conduit accelerates peripheral nerve regeneration by mimicking anatomical structure.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71791"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Responsive Bidirectional Actuator for Biofilm Disruption on Medical Surfaces. 用于医学表面生物膜破坏的双响应双向驱动器。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71804

Amit Kumar, Anas Saifi, Kumari Kiran, Sanjib Senapati, Nitish R Mahapatra, Pijush Ghosh

{"title":"Dual-Responsive Bidirectional Actuator for Biofilm Disruption on Medical Surfaces.","authors":"Amit Kumar, Anas Saifi, Kumari Kiran, Sanjib Senapati, Nitish R Mahapatra, Pijush Ghosh","doi":"10.1002/adhm.71804","DOIUrl":"https://doi.org/10.1002/adhm.71804","url":null,"abstract":"Biofilms, dense microbial communities embedded in extracellular polymeric substances, present persistent challenges in medical devices by conferring antibiotic resistance and fostering chronic infections. Conventional antimicrobial or enzymatic approaches often fail due to poor penetration into biofilm matrices. This work introduces a dynamic surface capable of mechanically disrupting biofilms, enabling on-demand cleaning of biomedical interfaces. Here, we present a dual-responsive bilayer actuator composed of a solvent-responsive hydrogel and a photoresponsive elastomer. The system harnesses solvent- and light-induced bidirectional bending for effective detachment of bacterial biofilms. The bilayer undergoes upward curvature upon exposure to solvent and reverses to downward curvature upon irradiation with green light (530 nm), demonstrating repeatable and directional actuation. We evaluate the removal of Escherichia coli and Staphylococcus aureus biofilms across 12, 24, and 48 h growth periods, comparing single and cyclic actuation modes. Quantitative analyses show that upward bending (θ∼ 156°) and downward bending (θ∼ -73.7°) each achieve significant detachment, while cyclic actuation further enhances removal efficiency (>70%), particularly for mature 48 h biofilms. Colony-forming unit assays validate the reduction in viable bacteria following the actuation. This study demonstrates the potential of dual-responsive actuators as biocompatible, nonchemical strategies for biofilm removal for next-generation antifouling medical device surfaces.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71804"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Reference Mapping and Transcriptomic Analysis Identify Fibroblast-Related ECM Remodeling During Electroacupuncture Treatment for Tendon Repair. 单细胞参考图谱和转录组学分析鉴定电针治疗肌腱修复过程中成纤维细胞相关的ECM重塑。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71774

Yang Wu, Boyun Lu, Zitian Zheng, Yucheng Zhu, Xinrui Fu, Meng Yang, Zhenghui Qiu, Chong Zhang, Jin Cheng, Jianquan Wang, Hongjie Huang

{"title":"Single-Cell Reference Mapping and Transcriptomic Analysis Identify Fibroblast-Related ECM Remodeling During Electroacupuncture Treatment for Tendon Repair.","authors":"Yang Wu, Boyun Lu, Zitian Zheng, Yucheng Zhu, Xinrui Fu, Meng Yang, Zhenghui Qiu, Chong Zhang, Jin Cheng, Jianquan Wang, Hongjie Huang","doi":"10.1002/adhm.71774","DOIUrl":"https://doi.org/10.1002/adhm.71774","url":null,"abstract":"Tendinopathy involves pathological extracellular matrix (ECM) remodeling and dysregulated fibroblast activity, yet the functional stratification of fibroblast subpopulations during disease and repair remains unclear. Although electroacupuncture (EA) shows clinical potential, its cellular mechanisms in tendon healing are not fully understood. Here, we integrated publicly available single-cell RNA sequencing datasets with bulk transcriptomic profiling from a murine Achilles tendinopathy model to establish a tendon cellular reference atlas and characterize MMP2+ fibroblast-associated ECM remodeling states, including High_ECM and Low_ECM programs. EA significantly improves pain sensitivity, locomotor function, and collagen organization while reducing fibrotic adhesions. Transcriptomic analysis reveals that EA reverses inflammatory and matrix-degradation pathways and upregulates collagen-assembly and ECM-receptor interaction genes. Integrated single-cell reference mapping and bulk transcriptomic analysis revealed that EA-associated tendon repair was accompanied by enrichment of ECM-related transcriptional programs overlapping with MMP2+ fibroblast-associated ECM remodeling states. Single-cell co-expression analysis identified coordinated expression patterns of key ECM-related genes. Our study suggests that EA-associated tendon repair is accompanied by modulation of ECM-related molecular programs overlapping with fibroblast-associated ECM remodeling states, providing new insights into the molecular framework underlying tendon repair after ECM injury.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71774"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetrahedral Framework Nucleic Acid-Based Small RNA Delivery: Prospects and Challenges in Bone and Cartilage Regeneration. 基于四面体框架核酸的小RNA递送：骨和软骨再生的前景和挑战。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71812

Liwei Fu, Zhengang Ding, Jiang Wu, Zhichao Zhang, Zhixing Zhang, Chuanyang Long, Jianhao Wu, Xiang Sui, Yongping Cao, Shuyun Liu, Quanyi Guo

{"title":"Tetrahedral Framework Nucleic Acid-Based Small RNA Delivery: Prospects and Challenges in Bone and Cartilage Regeneration.","authors":"Liwei Fu, Zhengang Ding, Jiang Wu, Zhichao Zhang, Zhixing Zhang, Chuanyang Long, Jianhao Wu, Xiang Sui, Yongping Cao, Shuyun Liu, Quanyi Guo","doi":"10.1002/adhm.71812","DOIUrl":"https://doi.org/10.1002/adhm.71812","url":null,"abstract":"Bone and cartilage defects represent major clinical challenges, with an increasingly urgent need for effective repair and regeneration. In recent years, small RNA-based regulatory strategies, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), have emerged as promising candidates for promoting bone and cartilage regeneration. However, achieving efficient and safe targeted delivery remains a critical challenge. Tetrahedral framework nucleic acids (tFNAs), a type of three-dimensional DNA nanomaterial with precise structure and excellent biocompatibility, serve dual functions as both intelligent delivery carriers and bioactive scaffolds, offering innovative solutions to this challenge. This review systematically outlines the advantages and research progress of tFNAs in this field, with a particular focus on their latest strategies and mechanisms as carriers for loading small RNAs that promote bone and cartilage regeneration. Additionally, the article summarizes cutting-edge investigations into enhancing the targeting ability, stability, and therapeutic efficacy of tFNAs through functional modifications and integration with biomaterials, and discusses the challenges and future directions for clinical translation.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71812"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Self-Reinforcing Hydrogel Disrupting Osteoclast Sealing Zone for Bone Erosion Alleviation. 一种自我强化水凝胶破坏破骨细胞封闭区以减轻骨侵蚀。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71808

Yilin Wei, Bei Kang, Baohong Liu, Valentin A Milichko, Wen Li, Yutong Song, Mengyao Zhang, Xinyi Liu, Haobo Wang, Zheyi Li, Weili Liu, Xinxing Wang, Nan Li

{"title":"A Self-Reinforcing Hydrogel Disrupting Osteoclast Sealing Zone for Bone Erosion Alleviation.","authors":"Yilin Wei, Bei Kang, Baohong Liu, Valentin A Milichko, Wen Li, Yutong Song, Mengyao Zhang, Xinyi Liu, Haobo Wang, Zheyi Li, Weili Liu, Xinxing Wang, Nan Li","doi":"10.1002/adhm.71808","DOIUrl":"https://doi.org/10.1002/adhm.71808","url":null,"abstract":"Abnormal remodeling of subchondral bone (SB), driven by osteoclast sealing zone formation and insufficient adaptation to joint mechanical stress, accelerates the progression of osteoarthritis (OA) and remains a major therapeutic challenge. Here, we developed a mechanically self-reinforcing injectable hydrogel in which amino-hydroxyapatite (amHap)-encapsulating spindle-shaped tellurium (Te) nanoparticles (Te@amHap, Team) were embedded within an oxidized alginate-gelatin matrix (Te@amHap/OG Gel, Team Gel). Team Gel contains dynamic Schiff base bonds, enabling controlled release of Team under joint mechanical forces. Within the acidic microenvironment of the osteoclast sealing zone, Team degrades and releases Te to be oxidized into TeO3 2- by osteoclast-derived H2O2. These ions can react with the thiol-containing residues of F-actin to form Te─S bonds, leading to F-actin degradation to disrupt the sealing zone and suppress bone resorption. Notably, Ca2+ from amHap interacts with alginate to form an egg-box secondary crosslinking structure, enhancing the crosslinking density beyond the original Schiff base network, thereby enhancing the mechanical strength of Team Gel and extending its retention time. Acting together, PO4 3 --mediated inhibition of osteoclast differentiation and Te-driven disruption of the mature osteoclast sealing zone suppress osteoclastogenesis and bone resorption. This sealing zone disruption strategy preserves bone quality and joint integrity, suggesting a promising osteoclast-targeted therapy for OA.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71808"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stretchable Microelectrode Arrays for Electro-Mechanical Probing of Brain Spheroids. 用于脑球体机电探测的可拉伸微电极阵列。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71788

Eleonora Martinelli, Ivan Furfaro, Flavius Timis, João Marques, Tala El Kaissi, Desirée Maulà, Laurine Kolly, Julian Bär, Sina Röllin, Scott Erickson, Laetitia Nikles, Benoît Delaup, Camille Delgrange, Loris Gomez Baisac, Luc Stoppini, Adrien Roux, Giuseppe Schiavone, Stéphanie P Lacour

{"title":"Stretchable Microelectrode Arrays for Electro-Mechanical Probing of Brain Spheroids.","authors":"Eleonora Martinelli, Ivan Furfaro, Flavius Timis, João Marques, Tala El Kaissi, Desirée Maulà, Laurine Kolly, Julian Bär, Sina Röllin, Scott Erickson, Laetitia Nikles, Benoît Delaup, Camille Delgrange, Loris Gomez Baisac, Luc Stoppini, Adrien Roux, Giuseppe Schiavone, Stéphanie P Lacour","doi":"10.1002/adhm.71788","DOIUrl":"https://doi.org/10.1002/adhm.71788","url":null,"abstract":"Mechanical forces shape brain development and contribute to pathological conditions such as traumatic brain injury. However, their direct effects on neural electrophysiology remain difficult to elucidate. Here, we introduce a multimodal system capable of delivering controlled mechanical actuation to brain spheroids while simultaneously recording their spontaneous electrophysiological activity. The platform enables culturing spheroids onto kirigami-patterned stretchable thin-film microelectrode arrays over multiple days. The array reliably records spontaneous spheroid activity and sustains uniaxial mechanical stimulation with controlled elongation and speed, ranging from manual precision stretching to high-velocity impacts. We capture spheroid deformation during slow stretch (∼100 µm/s) and detect acute electrophysiological changes following high-velocity stimulation (∼1-2 m/s). This multimodal platform establishes a foundational system for systematic in vitro investigations of how mechanical forces regulate functional activity in 3D tissues.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71788"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epitope-Imprinted Polymer Nanoparticles Enable Selective Recognition of Filamentous Vimentin for Tumor Imaging In Vivo. 表位印迹聚合物纳米颗粒能够选择性识别丝状静脉蛋白用于体内肿瘤成像。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71813

Kamaran Khurshid Dar, Yuan Li, Yongqin Lv

{"title":"Epitope-Imprinted Polymer Nanoparticles Enable Selective Recognition of Filamentous Vimentin for Tumor Imaging In Vivo.","authors":"Kamaran Khurshid Dar, Yuan Li, Yongqin Lv","doi":"10.1002/adhm.71813","DOIUrl":"https://doi.org/10.1002/adhm.71813","url":null,"abstract":"Vimentin is a cytoskeletal protein upregulated during epithelial-mesenchymal transition that can also be aberrantly displayed on the surface of multiple tumor cell types, making it a promising target for tumor imaging. Here, we develop epitope-imprinted polymer nanoparticles (nanoMIPs) for vimentin recognition through a two-step strategy comprising screening a library of non-imprinted nanoparticles to identify a lead formulation, followed by peptide-directed molecular imprinting. The resulting nanoMIPs exhibited enhanced apparent affinity for vimentin, with picomolar-range KD values, although these values represent model-dependent ensemble apparent affinities that may include contributions from multiple nanoparticle-ligand contacts and local rebinding rather than intrinsic monovalent affinities. The nanoMIPs preferentially bound cancer cells presenting cell-surface vimentin in vitro. The enhanced target binding and reduced off-target binding were consistent with an imprinting-associated improvement in molecular discrimination, although nonspecific physicochemical interactions were not completely eliminated. These findings extend epitope-guided nanoMIP design to structurally dynamic, cell surface-associated cytoskeletal targets, and support the development of synthetic receptors for tumor imaging. In vivo, the optimized nanoMIPs produced a tumor-associated fluorescence signal approximately 1.44 times that of the matched non-imprinted nanoparticles.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71813"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic-Inorganic Dual-Nanofiber Interpenetrating Network Scaffolds for Complementary Enhancement of Mechanical Stability and Osteogenic Bioactivity. 有机-无机双纳米纤维互穿网络支架互补增强机械稳定性和成骨生物活性。

IF 11 2区医学

Advanced Healthcare Materials Pub Date : 2026-10-06 DOI: 10.1002/adhm.71799

Pengfei Cai, Chunchun Li, Chengqiang Wang, Yangfan Ding, Jinglei Wu, Melanie L Hart, Bernd Rolauffs, Mohamed El-Newehy, Meera Moydeen Abdulhameed, Xiumei Mo, Binbin Sun, Lei Cao, Liang Song

{"title":"Organic-Inorganic Dual-Nanofiber Interpenetrating Network Scaffolds for Complementary Enhancement of Mechanical Stability and Osteogenic Bioactivity.","authors":"Pengfei Cai, Chunchun Li, Chengqiang Wang, Yangfan Ding, Jinglei Wu, Melanie L Hart, Bernd Rolauffs, Mohamed El-Newehy, Meera Moydeen Abdulhameed, Xiumei Mo, Binbin Sun, Lei Cao, Liang Song","doi":"10.1002/adhm.71799","DOIUrl":"https://doi.org/10.1002/adhm.71799","url":null,"abstract":"Incorporating nanofibers into 3D-printed scaffolds is an effective strategy to enhance the physicochemical properties and osteogenic bioactivity of bone tissue engineering constructs. In this study, we developed a composite scaffold featuring a dual-nanofiber interpenetrating network composed of silica (SiO2) and poly(L-lactic acid)/gelatin (PLLA/GEL) nanofibers. SiO2 nanofibers were employed to reinforce mechanical properties and release bioactive Si elements, establishing a favorable osteogenic microenvironment. Concurrently, PLLA/GEL nanofibers were chemically crosslinked with the sodium alginate (SA) matrix to stabilize the scaffold structure. This design effectively addresses the structural instability often observed in single-component inorganic fiber systems. Notably, systematic comparative experiments clarified the distinct contributions of each component. Results indicated that PLLA/GEL nanofibers primarily contributed to mechanical stability; however, their osteogenic contribution was not significantly superior to PLLA particles. In contrast, SiO2 nanofibers exhibited superior bioactivity compared to nanoparticles in inducing osteogenic differentiation and promoting pro-reparative macrophage polarization, leading to enhanced in vivo bone repair. This dual-nanofiber network complementarily improves mechanical stability, modulates inflammation, and promotes osteogenic efficacy, offering a novel strategy for designing high-performance functional bone tissue engineering scaffolds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71799"},"PeriodicalIF":11.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0