{"title":"生物杂交细菌作为活纳米工厂,通过抗菌-再生耦合破坏糖尿病伤口病理级联。","authors":"Wendi Xuan, Yupei Hu, Sicong Li, Xiaozhen Zhou, Meng Chen, Chenyao Wu, Xiang Gao, Gaoyan Xu, Jine Zhao, Lili Xia, Wei Feng, Yu Chen","doi":"10.1002/adhm.202503107","DOIUrl":null,"url":null,"abstract":"<p><p>Diabetic wound healing remains a formidable clinical challenge due to persistent biofilm formation, chronic inflammation, and excessive reactive oxygen species (ROS) accumulation. Current therapeutic approaches often lack synchronized antimicrobial-regenerative mechanisms and fail to provide sustained efficacy. Here, this work engineers a bioengineered living hydrogel system (BMB181@ALG) that leverages genetically modified Bacillus thuringiensis strain BMB181 as a melanin nanofactory, enabling in situ biosynthesis of multifunctional melanin nanoparticles (MNPs). Encapsulation within the hydrogel preserves bacterial metabolic activity, ensuring continuous MNPs production. These nanoparticles exhibit a dual-mode therapeutic action, including photothermal antibacterial activity under near-infrared irradiation for biofilm disruption and pathogen eradication, and ROS scavenging and antioxidant effects to modulate the inflammatory microenvironment. The sustained release of MNPs further promotes angiogenesis, enhances tissue regeneration, and dynamically regulates the diabetic wound microenvironment. Notably, the self-replenishing nature of this biohybrid system ensures long-term therapeutic efficacy, minimizing the need for frequent interventions. This study establishes a bacteria-driven therapeutic paradigm, demonstrating the translational potential of living microbial systems for next-generation precision wound management.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03107"},"PeriodicalIF":9.6000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biohybrid Bacteria as Living Nanofactories for Disrupting Diabetic Wound Pathological Cascade via Antimicrobial-Regenerative Coupling.\",\"authors\":\"Wendi Xuan, Yupei Hu, Sicong Li, Xiaozhen Zhou, Meng Chen, Chenyao Wu, Xiang Gao, Gaoyan Xu, Jine Zhao, Lili Xia, Wei Feng, Yu Chen\",\"doi\":\"10.1002/adhm.202503107\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Diabetic wound healing remains a formidable clinical challenge due to persistent biofilm formation, chronic inflammation, and excessive reactive oxygen species (ROS) accumulation. Current therapeutic approaches often lack synchronized antimicrobial-regenerative mechanisms and fail to provide sustained efficacy. Here, this work engineers a bioengineered living hydrogel system (BMB181@ALG) that leverages genetically modified Bacillus thuringiensis strain BMB181 as a melanin nanofactory, enabling in situ biosynthesis of multifunctional melanin nanoparticles (MNPs). Encapsulation within the hydrogel preserves bacterial metabolic activity, ensuring continuous MNPs production. These nanoparticles exhibit a dual-mode therapeutic action, including photothermal antibacterial activity under near-infrared irradiation for biofilm disruption and pathogen eradication, and ROS scavenging and antioxidant effects to modulate the inflammatory microenvironment. The sustained release of MNPs further promotes angiogenesis, enhances tissue regeneration, and dynamically regulates the diabetic wound microenvironment. Notably, the self-replenishing nature of this biohybrid system ensures long-term therapeutic efficacy, minimizing the need for frequent interventions. This study establishes a bacteria-driven therapeutic paradigm, demonstrating the translational potential of living microbial systems for next-generation precision wound management.</p>\",\"PeriodicalId\":113,\"journal\":{\"name\":\"Advanced Healthcare Materials\",\"volume\":\" \",\"pages\":\"e03107\"},\"PeriodicalIF\":9.6000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Healthcare Materials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1002/adhm.202503107\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Healthcare Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/adhm.202503107","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Biohybrid Bacteria as Living Nanofactories for Disrupting Diabetic Wound Pathological Cascade via Antimicrobial-Regenerative Coupling.
Diabetic wound healing remains a formidable clinical challenge due to persistent biofilm formation, chronic inflammation, and excessive reactive oxygen species (ROS) accumulation. Current therapeutic approaches often lack synchronized antimicrobial-regenerative mechanisms and fail to provide sustained efficacy. Here, this work engineers a bioengineered living hydrogel system (BMB181@ALG) that leverages genetically modified Bacillus thuringiensis strain BMB181 as a melanin nanofactory, enabling in situ biosynthesis of multifunctional melanin nanoparticles (MNPs). Encapsulation within the hydrogel preserves bacterial metabolic activity, ensuring continuous MNPs production. These nanoparticles exhibit a dual-mode therapeutic action, including photothermal antibacterial activity under near-infrared irradiation for biofilm disruption and pathogen eradication, and ROS scavenging and antioxidant effects to modulate the inflammatory microenvironment. The sustained release of MNPs further promotes angiogenesis, enhances tissue regeneration, and dynamically regulates the diabetic wound microenvironment. Notably, the self-replenishing nature of this biohybrid system ensures long-term therapeutic efficacy, minimizing the need for frequent interventions. This study establishes a bacteria-driven therapeutic paradigm, demonstrating the translational potential of living microbial systems for next-generation precision wound management.
期刊介绍:
Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.