Advanced Healthcare Materials最新文献_第9页

Advances in Photoacoustic Imaging for Brain Diseases: Principles, Applications, and Clinical Translation Challenges. 脑疾病光声成像的进展：原理、应用和临床转化挑战。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-12 DOI: 10.1002/adhm.202505743

Xixi Hu, Haoyang Chang, Shihan Xu, Yaozhong Jiang, Shuaikang Zhang, Zhaoyin Wang, Fan Wu, Zhihui Dai

{"title":"Advances in Photoacoustic Imaging for Brain Diseases: Principles, Applications, and Clinical Translation Challenges.","authors":"Xixi Hu, Haoyang Chang, Shihan Xu, Yaozhong Jiang, Shuaikang Zhang, Zhaoyin Wang, Fan Wu, Zhihui Dai","doi":"10.1002/adhm.202505743","DOIUrl":"https://doi.org/10.1002/adhm.202505743","url":null,"abstract":"The growing prevalence of brain diseases and the limitations of existing imaging techniques highlight the urgent demand for advanced, biocompatible, and high-resolution brain imaging approaches. Photoacoustic imaging (PAI) uniquely integrates scalable spatial resolution, deep penetration, and non-ionizing excitation, which enables multi-scale visualization from subcellular structures to the whole brain. This review systematically summarizes the fundamental principles of PAI, commonly used contrast agents, and key system components, and provides a comparative analysis of the major imaging modalities-optical-resolution and acoustic-resolution photoacoustic microscopy (OR-/AR-PAM), photoacoustic computed tomography (PACT), and photoacoustic endoscopy (PAE). PACT offers superior penetration depth and field of view, whereas OR-/AR-PAM provides higher spatial resolution at molecular scales. By aligning disease-specific imaging requirements with the functional strengths of each modality, PAI has enabled integrated structural and functional imaging in glioblastoma, neurodegenerative disorders, traumatic brain injury, and cerebrovascular diseases, highlighting its integrated advantages for multiscale brain imaging. Finally, the review discusses key challenges for clinical translation of PAI, including motion artifacts, blood-brain barrier constraints, contrast agent approval, limitations of single-modality approaches, and technical limitations, and proposes corresponding strategies to address these obstacles. This review provides forward-looking guidance for the clinical translation of multiscale brain imaging technologies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05743"},"PeriodicalIF":9.6,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for Nanomaterials to Circumvent the Mononuclear Phagocyte System. 纳米材料规避单核吞噬细胞系统的策略。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-12 DOI: 10.1002/adhm.71135

Bingyue Fu, Dawei Chen, Guohua Wang, Haiyang Hu, Ming Zhao

{"title":"Strategies for Nanomaterials to Circumvent the Mononuclear Phagocyte System.","authors":"Bingyue Fu, Dawei Chen, Guohua Wang, Haiyang Hu, Ming Zhao","doi":"10.1002/adhm.71135","DOIUrl":"https://doi.org/10.1002/adhm.71135","url":null,"abstract":"Nanomaterials (NMs) have emerged as promising tools for tumor-targeted therapy and gene editing. However, their delivery efficiency is severely limited by the mononuclear phagocyte system (MPS), which rapidly clears them from circulation primarily in the liver and spleen. This remains a major bottleneck for the clinical translation of nanomedicine. In order to break through this challenge, this review systematically summarizes main categories of strategies designed to circumvent MPS clearance. First, we discuss the strategies of engineering NMs to evade MPS recognition, including modulation of physicochemical property control, surface engineering strategy, and cell-based delivery systems. Second, we analyze the strategies that transiently modulate the MPS function itself, including macrophage blockade, phagocytosis inhibition, and macrophage depletion. These approaches collectively aim to reduce nonspecific clearance, thereby enhancing the accumulation of NMs at the target site. Finally, we further discuss the opportunities and remaining challenges in this field. This review is expected to provide a valuable reference for advancing the biomedical application of nanomaterials.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e71135"},"PeriodicalIF":9.6,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery System Based on Akkermansia Muciniphila Loaded Salidroside for the Treatment of Osteoporosis in Zebrafish Model. 载红景天苷的载药系统治疗斑马鱼骨质疏松模型。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-12 DOI: 10.1002/adhm.202504437

Jiahui Ma, Jing Ruan, Jia Yi, Shenghao Zhang, Yilei Ma, Rongbing Chen, Hanbing Wang, Li Luo, Bin Fang, Wei Wu, Qinsi Yang, Da Sun

{"title":"Delivery System Based on Akkermansia Muciniphila Loaded Salidroside for the Treatment of Osteoporosis in Zebrafish Model.","authors":"Jiahui Ma, Jing Ruan, Jia Yi, Shenghao Zhang, Yilei Ma, Rongbing Chen, Hanbing Wang, Li Luo, Bin Fang, Wei Wu, Qinsi Yang, Da Sun","doi":"10.1002/adhm.202504437","DOIUrl":"https://doi.org/10.1002/adhm.202504437","url":null,"abstract":"Glucocorticoid-induced osteoporosis (GIOP) is a frequent cost of dexamethasone (Dex) therapy; salidroside (SAL) shows promise but suffers from rapid clearance and poor oral bioavailability. We present an oral, living co-therapy in which viable Akkermansia muciniphila is vacuum-loaded with intracellular SAL and protected by a pH-responsive Eudragit L100 enteric coat (SAL@AKK@EL100). This construct effectively retains probiotic activity, shields the cargo through gastric transit, and shows prolonged intracellular retention with gradual release. In Dex-challenged zebrafish, SAL@AKK@EL100 reversed behavioral dysregulation and improved skeletal mineralization, outperforming SAL or AKK alone. Integrative RNA-seq/16S analysis revealed that SAL@AKK@EL100 treatment was associated with modulation of pathways related to tight junctions, ECM-receptor interaction, actin cytoskeleton, glutathione metabolism, and unsaturated fatty acid biosynthesis, alongside remodeling of the gut microbiota. SAL@AKK@EL100 establishes a modular, microbiome-anchored platform that couples probiotic viability with drug gradual release for promising oral management of GIOP.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e04437"},"PeriodicalIF":9.6,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piezoelectric Surface Charge and Dynamic Stimulation Synergize to Promote Cardiac Myoblast Alignment and Maturation. 压电表面电荷和动态刺激协同促进成心肌细胞排列和成熟。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-11 DOI: 10.1002/adhm.202505241

Rafaela M Meira, Sylvie Ribeiro, Senentxu Lanceros-Mendez, Clarisse Ribeiro

{"title":"Piezoelectric Surface Charge and Dynamic Stimulation Synergize to Promote Cardiac Myoblast Alignment and Maturation.","authors":"Rafaela M Meira, Sylvie Ribeiro, Senentxu Lanceros-Mendez, Clarisse Ribeiro","doi":"10.1002/adhm.202505241","DOIUrl":"https://doi.org/10.1002/adhm.202505241","url":null,"abstract":"This work investigates the role of surface charge and synergistic mechano-electrical stimulation of piezoelectric poly(vinylidene fluoride) (PVDF) on the behaviour of H9c2 cardiac cells. H9c2 cells preferentially adhered to poled PVDF samples, regardless of polarity, indicating that increased average surface charge creates a favourable environment for initial attachment. Proliferation assays showed that negatively charged PVDF films promote the highest early growth, while dynamic mechanical stimulation (1 h/day) further enhanced proliferation, particularly on positively poled PVDF, highlighting a synergistic effect between charge and mechanical cues. Differentiation assays demonstrated that stimulation applied during either the proliferation or differentiation phase promoted myotube formation, increasing length, width, and alignment, with negatively poled PVDF supporting the most advanced maturation. At the molecular level, qPCR confirmed upregulation of the early cardiomyogenic marker actc1, whereas the late marker Tnnt2 remained unchanged, indicating partial but incomplete cardiomyocyte differentiation. These results reveal that both static polarization and dynamic piezoelectric stimulation cooperatively modulate cell fate decisions, integrating electrical and mechanical signalling pathways. Together, these findings demonstrate that surface polarization and mechano-electrical cues of piezoelectric biomaterials act in concert to regulate cardiomyoblast adhesion, proliferation, and differentiation, unveiling charge-dependent design principles for multifunctional electroactive materials aimed at cardiac tissue regeneration.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05241"},"PeriodicalIF":9.6,"publicationDate":"2026-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147653415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-Targeted Liposomes Boost Thermogenesis for Adipose Tissue Regulation. 线粒体靶向脂质体促进脂肪组织调节的产热作用。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-11 DOI: 10.1002/adhm.202505925

Shibo Tian, Hailong Xie, Qin Zhong, Chenglu Sun, Peng Yuan, Funeng Xu, Lu Lu, Jiaxue Cao, Mingzhou Li, Haohuan Li

{"title":"Mitochondria-Targeted Liposomes Boost Thermogenesis for Adipose Tissue Regulation.","authors":"Shibo Tian, Hailong Xie, Qin Zhong, Chenglu Sun, Peng Yuan, Funeng Xu, Lu Lu, Jiaxue Cao, Mingzhou Li, Haohuan Li","doi":"10.1002/adhm.202505925","DOIUrl":"https://doi.org/10.1002/adhm.202505925","url":null,"abstract":"Mitochondria act as central organelles regulating energy metabolism and lipogenesis in adipocytes. Mitochondrial dysfunction and oxidative stress are closely implicated in the pathogenesis of obesity. Therefore, targeting adipose mitochondrial function to reduce mitochondrial reactive oxygen (mROS) levels can enhance mitochondrial activity and promote thermogenesis, presenting a promising therapeutic strategy for obesity. In this study, we developed cationic liposomes (AE@PEP-Lip) specifically designed for adipose mitochondrial targeting. These liposomes can effectively accumulate in white adipose tissue due to their highly positively charged surface, and subsequently localize to mitochondria via the mitochondrial-targeting peptide SS-31. The SS-31 peptide efficiently scavenges mROS and synergizes with empagliflozin in AE@PEP-Lip to inhibit mitochondrial fragmentation, thereby enhancing mitochondrial respiration and thermogenic capacity. Furthermore, allicin, which is loaded into AE@PEP-Lip and released into the cytoplasm, activates the AMPK signaling pathway and upregulates uncoupling proteins, further promoting mitochondrial thermogenesis. In obese mice, treatment with AE@PEP-Lip improved mitochondrial morphology and function and induced browning characteristics. Moreover, we demonstrated the clinical application potential of AE@PEP-Lip in reducing localized fat deposition using a porcine model. Taken together, this study presents a novel and promising therapeutic platform for obesity by modulating adipose mitochondrial function.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05925"},"PeriodicalIF":9.6,"publicationDate":"2026-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147653419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitin Derivative-Based Detachable Microneedles for Effective Treatment of Androgenic Alopecia. 基于几丁质衍生物的可拆卸微针有效治疗雄激素性脱发。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-10 DOI: 10.1002/adhm.202504691

Jiaxue Zhang, Yijia Zhang, Ying Yang, Wenhui Bai, Zhiqi Wang, Chen Yang, Peipei Zhang, Qingqing Li, Jiye Zhang, Jia Ma

{"title":"Chitin Derivative-Based Detachable Microneedles for Effective Treatment of Androgenic Alopecia.","authors":"Jiaxue Zhang, Yijia Zhang, Ying Yang, Wenhui Bai, Zhiqi Wang, Chen Yang, Peipei Zhang, Qingqing Li, Jiye Zhang, Jia Ma","doi":"10.1002/adhm.202504691","DOIUrl":"https://doi.org/10.1002/adhm.202504691","url":null,"abstract":"Androgenic alopecia (AGA) is the most common type of hair loss in clinical practice, which not only compromises scalp physiology but also induces psychological comorbidities. Despite its high prevalence, current therapeutic interventions for AGA remain constrained by prolonged treatment durations, notable safety risks, and prohibitive expenses. To address these limitations, we engineered detachable microneedles incorporating TEMPO-oxidized chitin derivatives (CD MNs), which demonstrated unprecedented hair regenerative capacity. In C57BL/6j AGA models, CD MNs induced 71.56% ± 14.20% hair regrowth density at day 16 (vs. 32.23% ± 14.55% for 5% minoxidil, the current clinical gold standard). Ex vivo AGA hair follicle exhibited 0.93 ± 0.15 mm hair shaft elongation under chitin derivatives treatment within 5 days, which is comparable to minoxidil. Mechanistic profiling via single-cell RNA sequencing identified CD MNs mediated suppression of lysosomal hydrolases and membrane proteins, indicating autophagy potentiation. Further ex vivo studies confirmed that chitin derivatives promoted hair growth by activating the hair follicle autophagy. Notably, the microneedle system exhibited no detectable dermal or visceral toxicity. To our knowledge, this study is the first to report the anti-AGA activity of chitin derivatives. Our findings established a robust theoretical foundation and preclinical validation for chitin-based biomaterials as next-generation AGA therapies, offering a safe, cost-effective alternative to conventional therapies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e04691"},"PeriodicalIF":9.6,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony-Forming Cells. 带有固定化抗体和生物活性肽的双功能表面介导内皮细胞集落形成的选择性捕获和增殖。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-09 DOI: 10.1002/adhm.202505092

Hugo A Level, Marc-Antoine Campeau, Mohamed A Elkhodiry, Gaétan Laroche, Jean-François Tanguay, Corinne A Hoesli

{"title":"Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony-Forming Cells.","authors":"Hugo A Level, Marc-Antoine Campeau, Mohamed A Elkhodiry, Gaétan Laroche, Jean-François Tanguay, Corinne A Hoesli","doi":"10.1002/adhm.202505092","DOIUrl":"10.1002/adhm.202505092","url":null,"abstract":"Endothelial colony-forming cells (ECFCs) are of significant interest in vascular biomaterials engineering and cell-based therapies for cardiovascular diseases. Efficient isolation and expansion of ECFCs on polymeric surfaces either in vitro or in situ in the case of implants could significantly advance ECFC-based therapies. We present a novel bifunctional surface modification strategy combining oriented antibodies and extracellular matrix-derived peptides to selectively capture ECFCs on surfaces and then mediate firm adhesion, spreading and proliferation. Studies were conducted with anti-CD309 antibodies and custom RGD peptides, previously shown to respectively enable ECFC capture and clonal expansion. To aid in oriented antibody immobilization on surfaces while modulating antibody-RGD surface concentrations, Fc-binding peptides and RGD peptides were co-immobilized on aminated surfaces using click chemistry, followed by affinity-mediated antibody immobilization. Bifunctional anti-CD309 + RGD surfaces selectively captured ECFCs from a mixture with peripheral blood mononuclear cells in dynamic conditions. The presence of RGD peptides significantly enhanced cell spreading and proliferation, leading to additive effects on surface coverage under flow. Proof-of-concept studies demonstrated successful adaptation to 3D polystyrene microcarriers, showcasing potential scalability for clinical-grade cell production. The surface modification scheme provides a versatile and clinically translatable platform for advancing ECFC-based regenerative therapies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05092"},"PeriodicalIF":9.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper-Collagen Interactions Regulate the Mechanical and Invasive Properties of Tumor Spheroids. 铜-胶原蛋白相互作用调节肿瘤球体的力学和侵袭特性。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-09 DOI: 10.1002/adhm.202505120

Paula Guerrero-López, Jose I Garcia-Peiro, Felipe Hornos, Jose L Hueso, Jesus Santamaria, J Manuel Garcia-Aznar

引用次数: 0

Multitalented Hybrid Targeted Nanoconstructs Selectively Repress Survival Genes and Induce Breast Tumor Regression In Vivo. 多天才杂交靶向纳米结构在体内选择性抑制存活基因并诱导乳腺肿瘤消退。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-09 DOI: 10.1002/adhm.202505296

Niladri Haldar, Rajkumar Samanta, Surajit Patra, Devyani Sengar, Sachin Jadhav, Virendra Gajbhiye

{"title":"Multitalented Hybrid Targeted Nanoconstructs Selectively Repress Survival Genes and Induce Breast Tumor Regression In Vivo.","authors":"Niladri Haldar, Rajkumar Samanta, Surajit Patra, Devyani Sengar, Sachin Jadhav, Virendra Gajbhiye","doi":"10.1002/adhm.202505296","DOIUrl":"https://doi.org/10.1002/adhm.202505296","url":null,"abstract":"Dysregulation of survival genes is a significant factor in the development of breast cancer and other cancers. Transcriptional downregulation of these genes offers a promising therapeutic strategy for cancer treatment by inducing apoptosis. Reducing expressions of these genes by RNA interference-mediated silencing is a potential strategy for more effective gene suppression. The research developed a novel protamine biopolymer-modified GSH-responsive biodegradable mesoporous silica nanoparticle system to suppress MCL-1 and Survivin genes' transcriptional activity in MCF-7 tumor-bearing mice. The nanocarriers were characterized using techniques like DLS, XRD, FTIR, GSH-utilization, SEM, and TEM. The nanocarriers were tested for their ability to deliver siRNAs into target cells, and their toxic behavior was assessed in vitro and in vivo. The nanocarriers' gene silencing and anti-cancer efficacy were assessed in both in vitro and in vivo models. The study revealed that synthesized MUC1 aptamer-conjugated protamine-modified nanocarriers (MPPM) offer stability to siRNAs, target MCF-7 tumor, and induce apoptosis with dual siRNAs. Additionally, the MPPM nanocarrier demonstrated significant potential for targeting the MCF-7 tumor in the mice model, and the histological findings suggested its biosafety profile. The siMCL-1/siSur@MPPM nanocarriers demonstrated a significant anti-tumor effect in an in vivo mice model through the silencing of target genes and the induction of apoptosis. These results indicate that the biodegradable siMCL-1/siSur@MPPM nanocarrier provides a significant combination of targeted delivery, biodegradability, effective gene silencing, and reduced off-target effects, suggesting its potential as a promising nanomedicine for breast cancer treatment.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05296"},"PeriodicalIF":9.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery of Engineered BMP2 circRNA via Biomimetic Nanovesicles Enhances Titanium Implant Osseointegration Through Translation-Controlled Osteogenesis. 通过仿生纳米囊泡传递工程化的BMP2环状rna，通过翻译控制的成骨促进钛种植体骨整合。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2026-04-09 DOI: 10.1002/adhm.202505504

Yichen Li, Ziyan Guo, Pengyu Zhao, Yan Dong, Mei Yong, Qi Li, Liang Kong, Zhongshan Wang

{"title":"Delivery of Engineered BMP2 circRNA via Biomimetic Nanovesicles Enhances Titanium Implant Osseointegration Through Translation-Controlled Osteogenesis.","authors":"Yichen Li, Ziyan Guo, Pengyu Zhao, Yan Dong, Mei Yong, Qi Li, Liang Kong, Zhongshan Wang","doi":"10.1002/adhm.202505504","DOIUrl":"https://doi.org/10.1002/adhm.202505504","url":null,"abstract":"Although mRNA therapy has achieved favorable outcomes, it still faces limitations such as poor stability and short duration of protein expression. In this study, we utilized a covalently closed circular RNA encoding bone morphogenetic protein-2 (BMP2 circRNA), which exhibits exceptional nuclease resistance and an extended half-life, thereby enabling sustained and efficient BMP2 protein expression. The BMP2 circRNA was encapsulated into biomimetic nanovesicles (BNVs) derived from bone marrow mesenchymal stem cells (BMSCs) using co-extrusion technology. These BNVs were then anchored onto the surface of micro-arc oxidized titanium (Ti-MAO) implants via polydopamine (PDA) adhesion, constructing a novel local gene delivery system. In vitro experiments confirmed that this system is not only efficiently internalized by cells but also evades lysosomal degradation, facilitating the sustained release of BMP2 protein. This, in turn, significantly promoted osteogenic gene expression and accelerated mineral deposition. Furthermore, in vivo animal studies demonstrated that the functionalized implant markedly enhanced bone regeneration, increasing both bone volume fraction and bone-to-implant contact. This study successfully integrated the inherent stability of circRNA with a biomimetic delivery strategy, offering an effective approach for improving implant osseointegration.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e05504"},"PeriodicalIF":9.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0