Advanced Healthcare Materials最新文献_第8页

Conditioned Media from Mechanically Stimulated Macrophages Upregulate Osteogenic Genes in Human Mesenchymal Stromal Cells. 机械刺激巨噬细胞条件培养基上调人间充质间质细胞成骨基因。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-22 DOI: 10.1002/adhm.202500706

Anne Géraldine Guex, Ursula Menzel, Yann Ladner, Angela R Armiento, Martin J Stoddart

{"title":"Conditioned Media from Mechanically Stimulated Macrophages Upregulate Osteogenic Genes in Human Mesenchymal Stromal Cells.","authors":"Anne Géraldine Guex, Ursula Menzel, Yann Ladner, Angela R Armiento, Martin J Stoddart","doi":"10.1002/adhm.202500706","DOIUrl":"https://doi.org/10.1002/adhm.202500706","url":null,"abstract":"Bone healing is a multifaceted scenario with tightly orchestrated sequences that decide upon successful bone fracture healing or nonunion. In this context, the immune system, particularly the impact of macrophages on mesenchymal stromal cell (MSC) recruitment and differentiation, cannot be overemphasized. Further adding to the complexity, fracture healing is not only governed by chemical signals but strongly depends on mechanical stimulation. Here, an in-house built bioreactor is used to culture THP-1 macrophages, stimulated with lipopolysaccharide and interferon-gamma (M(LPS)) or interleukin 4 (M(IL-4)) in fibrin hydrogels under compression and shear. Subsequently, MSC-pellets are cultured in conditioned media, derived from macrophages, and analyzed for chondrogenic or osteogenic gene expression after 9 days. In M(IL-4) conditions under mechanical load, expression of IL1B, IL6, TNF, IL10, CCL18, CD163, and CD206 is increased compared to the static condition. In MSCs, osteogenic genes RUNX2 and ALPL as well as chondrogenic genes ACAN and COL2A1 are increased in conditions treated with a medium derived from mechanically stimulated macrophages. The results suggest that culture in fibrin and under loading induces a complex macrophage polarization phenotype which affects processes during endochondral ossification in human MSC.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2500706"},"PeriodicalIF":10.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Antibacterial and Anti-Inflammatory Hemostatic Adhesive with Dual Zn²⁺ and NO Release for the Neurovascular Electrostimulation of Infected Diabetic Wounds. 一种双Zn2+和NO释放的抗菌抗炎止血胶粘剂用于糖尿病感染创面神经血管电刺激。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-20 DOI: 10.1002/adhm.202502097

Wenlong Wei, Jiayi Zheng, Zhenwu Zhong, Wen Ding, Yuanling Huang, Ziru Liu, Xiangyu Chen, Wenyue Yang, Sui Shi, Bei Jin, Pier-Luc Tremblay, Tian Zhang

{"title":"An Antibacterial and Anti-Inflammatory Hemostatic Adhesive with Dual Zn2+ and NO Release for the Neurovascular Electrostimulation of Infected Diabetic Wounds.","authors":"Wenlong Wei, Jiayi Zheng, Zhenwu Zhong, Wen Ding, Yuanling Huang, Ziru Liu, Xiangyu Chen, Wenyue Yang, Sui Shi, Bei Jin, Pier-Luc Tremblay, Tian Zhang","doi":"10.1002/adhm.202502097","DOIUrl":"10.1002/adhm.202502097","url":null,"abstract":"Diabetic wounds are difficult to heal because chronic infection, reactive oxygen species (ROS) in excess, and persistent inflammation repeatedly damage skin structures including blood vessels and nerves. Efficacious dressings developed for treating diabetic wounds should address these issues concurrently. Here, a multifunctional hydrogel adhesive system made by photocrosslinking arginine-functionalized methacrylated chitosan with gallic-acid-functionalized gelatin methacryloyl is reported. The resulting scaffold (ACGG) is loaded with a laponite delivery system gorged with Zn2+ (Zn@LAP; ZL). Because of the synergy between its components, the complete hydrogel, ACGG-ZL, is robust, flexible, adhesive, biodegradable, and conductive, which is an essential feature for skin wound treatment via electrostimulation (ES). Most importantly, ACGG-ZL released in an intelligent manner two bioactive molecules upon exposure to ROS: antibacterial, antioxidative, and anti-inflammatory NO and Zn2+. In vitro assays indicates that ACGG-ZL eliminates pathogens, including MRSA, and promotes the proliferation of fibroblasts, endothelial cells, and neuronal precursors, which are all involved in skin tissue remodeling. When combined with ES, hemostatic ACGG-ZL accelerated significantly infected skin wound healing in a diabetic animal model by clearing pathogens, quenching inflammation, stimulating fibroblasts, and promoting neurovascular tissue regeneration. Overall, ACGG-ZL with ES provides a multifunctional, efficacious approach for the treatment of complex diabetic injuries.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2502097"},"PeriodicalIF":10.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro. 三维成熟脂肪细胞-巨噬细胞共培养体外诱导炎症的实验研究。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-20 DOI: 10.1002/adhm.202500779

Sophia Nowakowski, Svenja Nellinger, Franziska Brigitte Albrecht, Petra Juliane Kluger

{"title":"Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro.","authors":"Sophia Nowakowski, Svenja Nellinger, Franziska Brigitte Albrecht, Petra Juliane Kluger","doi":"10.1002/adhm.202500779","DOIUrl":"10.1002/adhm.202500779","url":null,"abstract":"Adipose tissue inflammation plays a central role in the pathogenesis of metabolic disorders. It is closely associated with immune cell infiltration, particularly macrophages, and the release of pro-inflammatory cytokines. Reliable in vitro test systems that mimic the inflamed environment while being free of animal-derived components are essential to explore new treatments for obesity-related diseases. This study aims to develop a straightforward, animal-free adipocyte-macrophage co-culture for investigating adipose tissue inflammation. Therefore, the human monocytic cell lines Mono Mac (MM6) and THP-1 are co-cultured with human primary mature adipocytes (ACs) encapsulated in gellan gum (GG) within a defined environment. Both monocytic cell lines are effectively activated by phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) in the defined medium, exhibiting distinct cytokine profiles. A comparison between collagen and GG demonstrates that GG is a suitable animal-free matrix material for ACs. PMA+LPS successfully activates the 3D adipocyte-macrophage co-culture to an inflammatory state for 72 h in the developed defined medium. Viability and intracellular lipid content remain high, and the functionality of ACs (perilipin A) in untreated models remains intact. This inflamed adipocyte-macrophage co-culture is easy to assemble and set up in a defined environment, making it a potential test system for anti-inflammatory treatment strategies.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2500779"},"PeriodicalIF":10.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments. 弹性和蛋白酶响应形状记忆水凝胶细丝的打印和改道。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-20 DOI: 10.1002/adhm.202502262

Philip Lifwergren, Viktoria Schoen, Sajjad Naeimipour, Lalit Khare, Anna Wunder, Hanna Blom, Jose G Martinez, Pierfrancesco Pagella, Anders Fridberger, Johan Junker, Daniel Aili

{"title":"Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments.","authors":"Philip Lifwergren, Viktoria Schoen, Sajjad Naeimipour, Lalit Khare, Anna Wunder, Hanna Blom, Jose G Martinez, Pierfrancesco Pagella, Anders Fridberger, Johan Junker, Daniel Aili","doi":"10.1002/adhm.202502262","DOIUrl":"10.1002/adhm.202502262","url":null,"abstract":"The fabrication of mechanically robust and reconfigurable hydrogel filaments remains a major challenge in biofabrication of perfusable architectures, dynamic tissue models, and complex 3D cell-laden constructs. Conventional extrusion-based bioprinting techniques generate filaments that are soft and fragile, limiting post-processing, scalability, and functional adaptability. Rerouting of Free-Floating Suspended Hydrogel Filaments (REFRESH) is introduced as a biofabrication strategy that integrates an aqueous two-phase system (ATPS)-compatible elastic extracellular matrix mimicking bioink material with a flexible printing and post-processing approach to overcome these constraints. This method enables the formation of highly elastic hydrogel filaments cross-linked via strain-promoted azide-alkyne cycloaddition (SPAAC) of bicyclo[6.1.0]non-4-yne-functionalized hyaluronan, exhibiting a strain at break exceeding 100%. The printed filaments maintain mechanical integrity during manual handling and post-processing using textile-inspired techniques, such as knotting and braiding, into reconfigurable 3D architectures. A distinct shape memory function enables programmed mechanical actuation and recovery of deformed structures. The hydrogel system supports high cell viability across multiple cell types and enables the fabrication of multicellular constructs with spatially defined organization. By incorporating protease-degradable cross-linkers, REFRESH-generated filaments function as sacrificial templates for perfusable tubular structures. This approach significantly expands the biofabrication design space, offering new possibilities for engineering vascularized tissues and complex hydrogel-based architectures.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2502262"},"PeriodicalIF":10.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poly(hydroxy-oxazolidone) Thermoplastic Elastomers for Safer, Greener and Customizable Blood-Contacting Medical Devices. 聚（羟基恶唑酮）热塑性弹性体，用于更安全，更环保和可定制的血液接触医疗设备。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-19 DOI: 10.1002/adhm.202502670

Sofia F Melo, Anna Pierrard, Fréderic Lifrange, Marco Caliari, Céline D'Emal, Margaux Debuisson, Haritz Sardon, Philippe Delvenne, Patrizio Lancellotti, Christophe Detrembleur, Christine Jérôme, Cécile Oury

{"title":"Poly(hydroxy-oxazolidone) Thermoplastic Elastomers for Safer, Greener and Customizable Blood-Contacting Medical Devices.","authors":"Sofia F Melo, Anna Pierrard, Fréderic Lifrange, Marco Caliari, Céline D'Emal, Margaux Debuisson, Haritz Sardon, Philippe Delvenne, Patrizio Lancellotti, Christophe Detrembleur, Christine Jérôme, Cécile Oury","doi":"10.1002/adhm.202502670","DOIUrl":"https://doi.org/10.1002/adhm.202502670","url":null,"abstract":"Thermoplastic elastomers (TPEs) of the polyurethane (PU)-type have broad applications in healthcare. However, these materials have a number of drawbacks. Their synthesis requires the use of toxic isocyanates. Their hemocompatibility remains insufficient, resulting in high rates of thrombotic complications of most common blood-contacting devices, which further increases the risk of infection. Here, we report the facile, up-scalable preparation of a greener non-isocyanate polyurethane (NIPU) TPE, poly(hydroxy-oxazolidone) (PHOx). We show that PHOx can be processed by multiple relevant manufacturing techniques, i.e., hot pressing, injection-molding, electrospinning, and additive manufacturing. In vitro hemocompatibility tests with human blood demonstrate better performance than a conventional medical grade PU. PHOx triggers less contact phase activation of coagulation, less plasma protein adsorption and less platelet adhesion than PU. The adhesion of Staphylococcus epidermidis is also reduced in the first 2 hours of contact as compared to PU. PHOx is neither hemolytic nor cytotoxic upon indirect or direct contact with endothelial cells or fibroblasts. Additionally, subcutaneous implantation of PHOx in rabbits for one and four weeks confirms in vivo biocompatibility and no material degradation. PHOx is therefore a highly valuable biomaterial and a potential isocyanate-free alternative to conventional PU-based TPEs for manufacturing customizable blood-contacting devices with improved hemocompatibility.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2502670"},"PeriodicalIF":10.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Xanthium-Inspired ZnO for Precision Antibacterial Therapy and Regeneration of Infected Wounds. 增强黄原激发氧化锌用于感染伤口的精确抗菌治疗和再生。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-18 DOI: 10.1002/adhm.202501009

Jiaojiao Zhu, Tiao Wen, Yunxiao Ma, Qingya Zeng, Yilan Lin, Yutian Liu, Shanshan Chen, Qiang Wang, Wenhu Zhou

{"title":"Enhanced Xanthium-Inspired ZnO for Precision Antibacterial Therapy and Regeneration of Infected Wounds.","authors":"Jiaojiao Zhu, Tiao Wen, Yunxiao Ma, Qingya Zeng, Yilan Lin, Yutian Liu, Shanshan Chen, Qiang Wang, Wenhu Zhou","doi":"10.1002/adhm.202501009","DOIUrl":"https://doi.org/10.1002/adhm.202501009","url":null,"abstract":"Bacterial infections significantly hinder wound healing. Despite the widespread use of antibiotics, their limited efficacy and the growing issue of drug resistance necessitate the development of new antibacterial agents with enhanced therapeutic effects and wound healing properties. We developed DNA-templated nano zinc oxide (ZnO) as an effective antibacterial wound treatment. Through systematic studies, we found both DNA nucleobases and phosphate backbone contribute to ZnO formation and stabilization. Using C20 DNA (20-base oligonucleotide) as the optimal stabilizer, we created uniformly sized ZnO nanoparticles. Subsequent interfacial modification with 15% hydrogen peroxide (H2O2) yielded H-ZnO with enhanced colloidal stability, photocatalytic activity, and bacterial adhesion. These modifications significantly increased the antibacterial properties of H-ZnO compared to ZnO, while also regulating Zn2+ release. The sustained release of Zn2+ not only enhanced the biosafety of the nanoparticles but also promoted wound healing. As a result, H-ZnO effectively promoted wound healing with reduced fibrotic response of both ordinary and bacterial-infected wounds without noticeable toxicity. The H-ZnO gel formulation demonstrated superior antibacterial activity and wound healing promotion, making it a promising candidate for clinical application in treating infected wounds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2501009"},"PeriodicalIF":10.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shear-Induced Patterning of Decellularized Skeletal Muscle Extracellular Matrix for Enhanced Myogenesis. 剪切诱导的脱细胞骨骼肌细胞外基质模式促进肌肉生成。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-18 DOI: 10.1002/adhm.202501357

Yong How Tan, Cynthia A Alcazar-Daleo, Jonah G Holbrook, Krista M Habing, Owen J Lally, Joshua C Vanderpool, Theo Seah, Renee Liu, Rashaad Ahsan, Leanna Li, Karina H Nakayama

{"title":"Shear-Induced Patterning of Decellularized Skeletal Muscle Extracellular Matrix for Enhanced Myogenesis.","authors":"Yong How Tan, Cynthia A Alcazar-Daleo, Jonah G Holbrook, Krista M Habing, Owen J Lally, Joshua C Vanderpool, Theo Seah, Renee Liu, Rashaad Ahsan, Leanna Li, Karina H Nakayama","doi":"10.1002/adhm.202501357","DOIUrl":"https://doi.org/10.1002/adhm.202501357","url":null,"abstract":"Severe skeletal muscle injuries often result in permanent functional deficits, posing a major clinical challenge; biomaterials that support cellular activity and provide instructive microenvironmental cues offer a promising strategy to enhance regeneration. To address this challenge, a novel engineering strategy is introduced to fabricate and pattern decellularized extracellular matrix (dECM) scaffolds with tunable biophysical properties. By leveraging pH-driven fibrillogenesis, combined with shear-based extrusion, controlled fibril assembly within skeletal muscle dECM, with precise topographical patterning of scaffold nanoarchitecture is demonstrated. This dual-modulation produces patterned scaffolds with compositionally mimetic ECM that direct myogenic cell alignment, influence cell phenotype, and facilitate scaffold remodeling. In a preclinical mouse model of volumetric muscle loss, these engineered dECM scaffolds promote the formation of new myofibers and enhance muscle regeneration, largely through the facilitation of scaffold and tissue remodeling for better integration. This work highlights the versatility of ECM-derived materials tailored to mimic the native composition of skeletal muscle, while also imparting new biophysical features that optimize myogenesis. By supporting tissue remodeling and functional integration, fibrillar patterned dECM represents a robust platform for advancing musculoskeletal regenerative therapies following traumatic injuries.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2501357"},"PeriodicalIF":10.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput 96-Well Nanogroove-Enhanced Electrical Impedance Biosensor for Real-Time Label-Free Cancer Drug Screening. 用于实时无标签癌症药物筛选的高通量96孔纳米沟槽增强电阻抗生物传感器

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-18 DOI: 10.1002/adhm.202402057

Jong Seob Choi, Hye-Bin Park, Su Han Lee, Byunggik Kim, Jihoon Lee, Sang-Keun Sung, Chia-Yi Su, JuKyung Lee, Seongjun Jang, Yongjin Lee, Jung Hyun Lee, Hyung Jin Kim, Deok-Ho Kim

{"title":"High-Throughput 96-Well Nanogroove-Enhanced Electrical Impedance Biosensor for Real-Time Label-Free Cancer Drug Screening.","authors":"Jong Seob Choi, Hye-Bin Park, Su Han Lee, Byunggik Kim, Jihoon Lee, Sang-Keun Sung, Chia-Yi Su, JuKyung Lee, Seongjun Jang, Yongjin Lee, Jung Hyun Lee, Hyung Jin Kim, Deok-Ho Kim","doi":"10.1002/adhm.202402057","DOIUrl":"https://doi.org/10.1002/adhm.202402057","url":null,"abstract":"This study advances bioelectronic platforms and cellular behavior analysis by enhancing the precision and scalability of nanopatterned membranes integrated with electrode arrays for real-time, high-throughput monitoring. By employing self-assembled monolayers (SAMs) and optimizing imprinting parameters, uniform large-area nanopatterns are successfully fabricated, overcoming challenges such as the \"rabbit ears\" effect and inconsistent pattern fidelity. The nanopatterned substrates, integrated within 96-well plates with electrode arrays, enable real-time impedance spectroscopy, providing a dynamic assessment of cellular behavior under chemotherapeutic drug exposure. The developed NanoIEA platform facilitates comprehensive investigations into cellular growth and drug interactions. RNA sequencing of MCF-7 cells cultured on nanopatterned substrates reveals significant differential gene expression, suggesting that traditional flat-surface cultures may induce artificial gene regulation, potentially biasing drug screening results. Patterned cell cultures that mimic physiological conditions yield more accurate and predictive outcomes for anticancer drug screening. This research underscores the critical role of nanopatterning in recapitulating in vivo-like gene expression and highlights the profound impact of microenvironmental cues on cellular behavior. By integrating advanced nanofabrication with precise real-time monitoring, this approach addresses technical limitations in bioelectronic sensing while providing deeper insights into dynamic cellular responses, reinforcing the importance of substrate design in tissue engineering and drug development.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402057"},"PeriodicalIF":10.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental Syncytium-on-Chip (PSoC)-Comparison of Forskolin or Mechanical Induced-Syncytialization. 胎盘芯片上合胞体(PSoC)-福斯克林与机械诱导合胞的比较。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-18 DOI: 10.1002/adhm.202404462

Ludivine Delon, Mathias Busek, Pedro Duarte Menezes, Nikolaj Gadegaard, Justyna Stokowiec, Alexey Golovin, Yuliia Boichuk, Thomas Combriat, Aleksandra Aizenshtadt, Stefan Krauss

{"title":"Placental Syncytium-on-Chip (PSoC)-Comparison of Forskolin or Mechanical Induced-Syncytialization.","authors":"Ludivine Delon, Mathias Busek, Pedro Duarte Menezes, Nikolaj Gadegaard, Justyna Stokowiec, Alexey Golovin, Yuliia Boichuk, Thomas Combriat, Aleksandra Aizenshtadt, Stefan Krauss","doi":"10.1002/adhm.202404462","DOIUrl":"https://doi.org/10.1002/adhm.202404462","url":null,"abstract":"The placenta is a key embryonic structure that separates maternal and fetal blood systems. The barrier function of the human placenta is performed by villous trophoblasts, i.e. undifferentiated cytotrophoblasts and differentiated syncytiotrophoblats, whose maturation and function are influenced by wall shear stress (WSS) from the maternal blood circulation. Most in vitro placenta models rely on cyclic adenosine monophosphate inducer forskolin (FSK) to establish a placental syncytium. Here a trophoblastic BeWo cell line is used to systematically compare the effect of FSK treatment in static culture with WSS stimulation in a pumpless, recirculating organ-on-chip. It is shown that BeWo cells undergo a similar differentiation under WSS exposure to FSK treatment. A WSS of 0.1 dyn cm-2 leads to cell fusion, polarization, barrier functions, human chorionic gonadotropin (β-hCG) secretion, and increased expression of key transporters. Moreover, WSS induces favorable changes in the levels of FMS-like tyrosine kinase-1 (FLT-1) and Placental Growth Factor (PlGF) suggesting the development of a physiologically relevant placental syncytium-on-chip (PSoC) without the need for FSK. The platform is further expanded to a syncytiotrophoblast/endothelial co-culture showing physiological vascular functions under WSS. The forskolin-free PSoC presented here represents the first pumpless recirculating and scalable platform for physiological placental studies and drug testing.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404462"},"PeriodicalIF":10.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable, Solvent Free Strontium Carbonate Poly(Allyl Glycidyl Ether Succinate) Composite Networks for Vertebral Augmentation. 可注射，无溶剂碳酸锶聚丙烯基缩水甘油醚琥珀酸酯复合网络椎体增强。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-06-18 DOI: 10.1002/adhm.202501633

Russell E Thompson, Maddison I Segal, Stephanie Sipics, Nicola G Judge, Alexia Bensoussan, Bavand Keshavarz, Matthew L Becker

{"title":"Injectable, Solvent Free Strontium Carbonate Poly(Allyl Glycidyl Ether Succinate) Composite Networks for Vertebral Augmentation.","authors":"Russell E Thompson, Maddison I Segal, Stephanie Sipics, Nicola G Judge, Alexia Bensoussan, Bavand Keshavarz, Matthew L Becker","doi":"10.1002/adhm.202501633","DOIUrl":"https://doi.org/10.1002/adhm.202501633","url":null,"abstract":"Vertebral body compression fractures are a major cause of chronic back pain, particularly in older adults. Augmentation is currently performed by injecting a poly(methyl methacrylate) (PMMA) slurry of polymer, monomer, and initiator mixed with barium sulfate (BaSO4) into the vertebrae, which then polymerizes in vivo. Herein, a solvent-free polymer system using poly(allyl glycidyl ether succinate) (PAGES) is developed for vertebral augmentation. PAGES crosslinks in situ through thiol-ene click chemistry with a cure time at 37 °C ranging from 17 to 53 min based on degree of polymerization and crosslinker concentration. The addition of SrCO3 increased the ultimate compressive strength (σmax) of the PAGES composite to 4.4 ± 0.4 MPa. Furthermore, SrCO3 increases osteoblast proliferation and differentiation of mesenchymal stem cells seeded onto the surface of PAGES composite. Finally, the compressive strength of fractured vertebrae is increased in an ex vivo surrogate rabbit model when filled with injected PAGES composite, demonstrating its potential as a bone augmentation material.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2501633"},"PeriodicalIF":10.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0