Advanced Healthcare Materials最新文献_第7页

The Printed Path to Healing: Advancing Wound Dressings through Additive Manufacturing. 打印路径愈合：通过增材制造推进伤口敷料。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202402711

Joana Galvão Duarte, Ana Paula Piedade, Bruno Sarmento, Filipa Mascarenhas-Melo

引用次数: 0

Silicene-Based Quantum Dots Nanocomposite Coated Functional UV Protected Textiles With Antibacterial and Antioxidant Properties: A Versatile Solution for Healthcare and Everyday Protection. 具有抗菌和抗氧化性能的硅基量子点纳米复合涂层功能性紫外线防护纺织品：用于医疗保健和日常保护的通用解决方案。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202404911

Poushali Das, Sayan Ganguly, Parham Khoshbakht Marvi, Shiza Hassan, Masoomeh Sherazee, Mohamed Mahana, Xiaowu Shirley Tang, Seshasai Srinivasan, Amin Reza Rajabzadeh

{"title":"Silicene-Based Quantum Dots Nanocomposite Coated Functional UV Protected Textiles With Antibacterial and Antioxidant Properties: A Versatile Solution for Healthcare and Everyday Protection.","authors":"Poushali Das, Sayan Ganguly, Parham Khoshbakht Marvi, Shiza Hassan, Masoomeh Sherazee, Mohamed Mahana, Xiaowu Shirley Tang, Seshasai Srinivasan, Amin Reza Rajabzadeh","doi":"10.1002/adhm.202404911","DOIUrl":"https://doi.org/10.1002/adhm.202404911","url":null,"abstract":"The predominant adverse health effects in care delivery result from hospital-acquired (nosocomial) infections, which impose a substantial financial burden on global healthcare systems. Integrating contact-killing antibacterial action, gas permeability, and antioxidant properties into textile coatings offers a transformative solution, significantly enhancing both medical and everyday protective applications. This study presents an innovative, pollution-free physical compounding method for creating a fluorescent biopolymer composite embedded with silicene-based heteroatom-doped carbon quantum dots for the production of functional textiles. The resulting coated fabric shows superior ultraviolet (UV) protection behavior (UVA and UVB), thermal stability, breathability, mechanical strength, and antioxidant capabilities as demonstrated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) experiment (>78%) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS assay (>90%). Rigorous testing against both gram positive and gram negative bacteria confirms that the coated fabric has excellent antibacterial activity. Results from time-dependent antibacterial assays indicate that the nanocomposite can markedly inhibit bacterial proliferation within a few hours. Molecular dynamics modeling, in conjunction with experimental investigations, is employed to elucidate the intermolecular interactions influencing the components of the treated cotton fabrics. The ongoing research can result in the creation of cost-effective smart textile substrates aimed at inhibiting microbial contamination in healthcare and medical applications, possibly rendering them commercially viable.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404911"},"PeriodicalIF":10.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional Carbon Dots In Situ Confined Hydrogel for Optical Communication, Drug Delivery, pH Sensing, Nanozymatic Activity, and UV Shielding Applications. 多功能碳点原位限制水凝胶用于光通信，药物输送，pH传感，纳米酶活性和紫外线屏蔽应用。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202403876

Parham Khoshbakht Marvi, Poushali Das, Arman Jafari, Shiza Hassan, Houman Savoji, Seshasai Srinivasan, Amin Reza Rajabzadeh

{"title":"Multifunctional Carbon Dots In Situ Confined Hydrogel for Optical Communication, Drug Delivery, pH Sensing, Nanozymatic Activity, and UV Shielding Applications.","authors":"Parham Khoshbakht Marvi, Poushali Das, Arman Jafari, Shiza Hassan, Houman Savoji, Seshasai Srinivasan, Amin Reza Rajabzadeh","doi":"10.1002/adhm.202403876","DOIUrl":"https://doi.org/10.1002/adhm.202403876","url":null,"abstract":"Inspired by the emerging potential of photoluminescent hydrogels, this work unlocks new avenues for advanced biosensing, bioimaging, and drug delivery applications. Carbon quantum dots (CDs) are deemed particularly promising among various optical dyes, for enhancing polymeric networks with superior physical and chemical properties. This study presents the synthesis of CDs derived from Prunella vulgaris, a natural plant resource, through a single-step hydrothermal process, followed by their uniform integration into hydrogel matrices via an in situ free radical graft polymerization. The resulting CD-integrated hydrogels exhibit multifunctionality in biomedical applications, featuring a diffusion-controlled drug release mechanism, permit concurrent delivery of photoluminescent CDs and therapeutic agents, enabling real-time monitoring over 32 h. In addition, these hydrogels function as a broad-range optical pH sensor (pH 3-11), provide robust ultraviolet (UV) shielding, and demonstrate nanozyme-like peroxidase activity. Critically, biocompatibility tests confirm their non-cytotoxicity toward fibroblast cells, establishing these hydrogels as promising candidates for diverse biomedical applications. These include advanced wound dressings that monitor the healing process and detect infection through pH sensing, and promote healing through the nanozymatic activity, all while maintaining a moist wound microenvironment. These hydrogels demonstrate exceptional suitability for advanced smart drug delivery, effective UV-blocking, and as innovative platforms for in vivo sensing and bioimaging.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403876"},"PeriodicalIF":10.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamically Assembling Magnetic Nanochains as New Generation of Swarm-Type Magneto-Mechanical Nanorobots Affecting Biofilm Integrity. 动态组装磁性纳米链作为影响生物膜完整性的新一代蜂群型磁机械纳米机器人。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202403736

Slavko Kralj, Charlotte Da Silva, Sebastjan Nemec, Maja Caf, Isabelle Fourquaux, Marie-Pierre Rols, Muriel Golzio, Alenka Mertelj, Jelena Kolosnjaj-Tabi

{"title":"Dynamically Assembling Magnetic Nanochains as New Generation of Swarm-Type Magneto-Mechanical Nanorobots Affecting Biofilm Integrity.","authors":"Slavko Kralj, Charlotte Da Silva, Sebastjan Nemec, Maja Caf, Isabelle Fourquaux, Marie-Pierre Rols, Muriel Golzio, Alenka Mertelj, Jelena Kolosnjaj-Tabi","doi":"10.1002/adhm.202403736","DOIUrl":"https://doi.org/10.1002/adhm.202403736","url":null,"abstract":"Bacterial resistance is gaining ground and novel, unconventional strategies are required to improve antibiotic treatments. As a synthetic analog of planktonic bacilli, the natural bacterial swimmers that can penetrate bacterial biofilms, ultra-short propelling magnetic nanochains are presented as bioinspired magnetic nanorobots, enhancing the antibiotic treatment in biofilm-forming Staphylococcus epidermidis. Propelling nanochains, activated by a low intensity (<20 mT) and low frequency (<10 Hz) rotating magnetic field (RMF), prompt the otherwise resistant biofilm-forming bacteria to become sensitive to methicillin, resulting in the killing of 99.99% of bacteria. While magnetic force-driven spherical magnetic nanoparticles were previously reported as unidirectional biofilm channel diggers, propelling nanochains emerge as second-generation magnetic nanorobots, which, due to their magnetic core, shape anisotropy, and negative zeta potential, combine magnetic responsiveness, torque-driven movement, and attractive electrostatic interactions to attach to bacterial aggregates and multi-directionally protrude throughout the biofilm, indulging mechanical forces. These synergistic effects, in combination with an antibiotic drug, destroy the bacterial extracellular matrix and eradicate the formed biofilm, as confirmed with several complementary techniques.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403736"},"PeriodicalIF":10.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Osseointegration via Endogenous Electric Field by Regulating the Charge Microenvironments around Implants. 内源电场调控种植体周围电荷微环境促进骨整合。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202403388

Fangfang Xu, Guangbin Zhao, Yuxin Gong, Xiang Liang, Ming Yu, Hao Cui, Linyang Xie, Nan Zhu, Xuan Zhu, Xiaoxi Shao, Kun Qi, Bingheng Lu, Junbo Tu, Sijia Na

{"title":"Enhancement of Osseointegration via Endogenous Electric Field by Regulating the Charge Microenvironments around Implants.","authors":"Fangfang Xu, Guangbin Zhao, Yuxin Gong, Xiang Liang, Ming Yu, Hao Cui, Linyang Xie, Nan Zhu, Xuan Zhu, Xiaoxi Shao, Kun Qi, Bingheng Lu, Junbo Tu, Sijia Na","doi":"10.1002/adhm.202403388","DOIUrl":"https://doi.org/10.1002/adhm.202403388","url":null,"abstract":"The regulation of the charged microenvironment around implants is an effective way to promote osseointegration. Although homeostasis of the charged microenvironment plays an integral role in tissues, current research is externally invasive and unsuitable for clinical applications. In this study, functional materials with different surface potential differences are prepared by changing the spatial layout of Ta and Ag on the surface of a Ti-6Al-4V alloy (TC4). This naturally formed an endogenous electric field (EEF) with a negatively charged cell membrane after in vivo implantation and promoted osseointegration at the interface between the bone and implant through the upregulation of Ca2+ concentration and activation of subsequent pathways. Interestingly, the promotion of stem cell differentiation, regulation of the direction of immune cell polarization, and antibacterial efficacy are determined by the free charge contained in the implant, rather than by the magnitude of the surface potential difference. This functional implant represents a unique strategy for regulating the charged microenvironment around the implant and enhancing osseointegration, thereby providing ideas and technical approaches for the clinical development of novel implant materials.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403388"},"PeriodicalIF":10.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycationic γ-Cyclodextrin with Amino Side Chains for a Highly Efficient Anti-Heparin Coagulant. 带氨基侧链的聚阳离子γ-环糊精制备高效抗肝素凝固剂。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-05 DOI: 10.1002/adhm.202404357

Wen-Jin An, Zhuo Lei, Xiao-Yong Yu, Chu-Han Liu, Chen Zhang, Yong Chen, Yu Liu

{"title":"Polycationic γ-Cyclodextrin with Amino Side Chains for a Highly Efficient Anti-Heparin Coagulant.","authors":"Wen-Jin An, Zhuo Lei, Xiao-Yong Yu, Chu-Han Liu, Chen Zhang, Yong Chen, Yu Liu","doi":"10.1002/adhm.202404357","DOIUrl":"https://doi.org/10.1002/adhm.202404357","url":null,"abstract":"Multicharged cyclodextrins have attracted significant attention because of their applications in biology and pharmaceuticals. This study reports an aminoethoxy-phenyl-pyridinium-modified γ-cyclodextrin (PyA-γ-CD) as a highly efficient coagulant for heparin through multivalent interactions. The UV titration experiment is performed to obtain apparent binding constants (Kobs) between PyA-γ-CD and heparin as high as 9.85 × 106 M-1. The activated partial thromboplastin time (aPTT) experiment in porcine plasma indicates that PyA-γ-CD not only exhibits nearly complete neutralization activity for unfractionated heparin (UFH), but more importantly, it also effectively neutralizes three LMWHs (dalteparin (Dalte), enoxaparin (Enoxa), and nadroparin (Nadro)) with a broader therapeutic window compared to protamine. The top neutralization activity of PyA-γ-CD for UFH, Dalte, Enoxa, and Nadro is 94%, 91%, 99%, and 85%, respectively. Interestingly, in vivo assays in mice further suggest that PyA-γ-CD significantly reverses the severe bleeding caused by heparin overdose while exhibiting remarkable biocompatibility. Therefore, PyA-γ-CD holds significant potential as a heparin antidote for clinical applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404357"},"PeriodicalIF":10.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-Controllable Release of Carbon Monoxide in Multifunctional Polymer Coating for Synergetic Treatment of Catheter-Related Infections. 多功能聚合物涂层超声可控一氧化碳释放协同治疗导管相关性感染。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-02 DOI: 10.1002/adhm.202403597

Ziqing Liang, Rui Sun, Xu Zhang, Shifang Luan, Hong Xu, Rui Wang, Lingjie Song, Hengchong Shi, Lei Wang

{"title":"Ultrasound-Controllable Release of Carbon Monoxide in Multifunctional Polymer Coating for Synergetic Treatment of Catheter-Related Infections.","authors":"Ziqing Liang, Rui Sun, Xu Zhang, Shifang Luan, Hong Xu, Rui Wang, Lingjie Song, Hengchong Shi, Lei Wang","doi":"10.1002/adhm.202403597","DOIUrl":"https://doi.org/10.1002/adhm.202403597","url":null,"abstract":"Medical catheters are susceptible to biological contamination and pathogen invasion, leading to infection and inflammatory complications. The development of antimicrobial coatings for medical devices has emerged as a promising strategy. However, limited biological functionality and the incompatibility between bactericidal properties and biosafety remain great challenges. Herein, a multifunctional polymer coating (CPB-Ac) is created, incorporating an ultrasonic-responsive carbon monoxide release unit (CORM-Ac) and antifouling unit to treat catheter-related complications. As-synthesized CPB-Ac polymer can be stably anchored to various medical devices with arbitrary shapes and compositions via facile UV treatment. Both in vivo and vitro experiments demonstrated that this multi-functional coating exhibits anti-fouling, anti-inflammatory, and broad-spectrum antibacterial activities as well as good biosafety. During the initial implantation phase, the antifouling units of CPB-Ac coating effectively inhibit the attachment of biological contaminants, significantly reducing the risk of thrombosis and bacterial infection. Once bacterial infection occurs, ultrasonic irradiation can activate CPB-Ac coating to release CO with a much higher amount of 55.3 µm than non-ultrasound controls, therefore rapidly eliminating bacteria and alleviating inflammatory response. It is believed that the work may provide an effective method for the development of next-generation intelligent medical coatings against catheter-related complications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403597"},"PeriodicalIF":10.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coacervate-Derived Assembly of Poly(ethylene glycol) Nanoparticles for Combinational Tumor Therapy. 聚乙二醇纳米粒子聚簇衍生组装用于肿瘤联合治疗。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-02 DOI: 10.1002/adhm.202403865

Hanru Liu, Dandan Ren, Huimin Geng, Yuan Tian, Mengqi Li, Ning Wang, Shiling Yuan, Jingcheng Hao, Jiwei Cui

{"title":"Coacervate-Derived Assembly of Poly(ethylene glycol) Nanoparticles for Combinational Tumor Therapy.","authors":"Hanru Liu, Dandan Ren, Huimin Geng, Yuan Tian, Mengqi Li, Ning Wang, Shiling Yuan, Jingcheng Hao, Jiwei Cui","doi":"10.1002/adhm.202403865","DOIUrl":"https://doi.org/10.1002/adhm.202403865","url":null,"abstract":"Coacervates have garnered significant attention as potential drug carriers. However, the instability resulting from their intrinsic membrane-free nature restricts the application of coacervates in drug delivery. Herein, the engineering of poly(ethylene glycol) nanoparticles (PEG NPs) is reported using coacervates composed of PEG and polyphenols as the templates, where PEG is subsequently cross-linked based on different chemistries (e.g., thiol-disulfide exchange, click chemistry, and Schiff base reaction). The reported assembly strategy avoids the template removal process and the resultant PEG NPs exhibit excellent stability in the physiological environment compared to coacervates. The presence of polyphenols in PEG NPs enables the loading of various cargos including metal ions (i.e., Ru, Gd, Mn, Fe) and drug molecules (i.e., doxorubicin), which demonstrates their promise in magnetic resonance imaging and combinational tumor therapy. This work provides a promising strategy to promote the development of coacervate-derived NPs as a drug delivery system for biomedical applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403865"},"PeriodicalIF":10.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose-Responsive Zn(II)-Porphyrin COF Adhesive Hydrogels With Dual-Active Sites and GOX-Like Activity for Accelerated Wound Healing. 具有双活性位点和gox样活性的葡萄糖反应性锌(II)-卟啉COF黏附水凝胶加速伤口愈合。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-02 DOI: 10.1002/adhm.202404076

Jin Wu, Tao Meng, Xiaoqian Zhang, Songtao Tang, Lei Liu, Jing Xue, Xiaohui Liu, Junjun Wang, Jiyue Wen, Danyou Hu, Guiyang Zhang

{"title":"Glucose-Responsive Zn(II)-Porphyrin COF Adhesive Hydrogels With Dual-Active Sites and GOX-Like Activity for Accelerated Wound Healing.","authors":"Jin Wu, Tao Meng, Xiaoqian Zhang, Songtao Tang, Lei Liu, Jing Xue, Xiaohui Liu, Junjun Wang, Jiyue Wen, Danyou Hu, Guiyang Zhang","doi":"10.1002/adhm.202404076","DOIUrl":"https://doi.org/10.1002/adhm.202404076","url":null,"abstract":"Effective glycemic control is paramount for optimal wound healing in diabetic patients. Traditional antibacterial and anti-inflammatory treatments, while important, often fall short in addressing the hyperglycemic conditions of diabetic wounds. Therefore, the development of novel therapeutic strategies for accelerating diabetic wound healing has garnered escalating attention. Covalent organic frameworks (COFs) are an emerging class of crystalline porous polymers constructed through strong covalent bonds. Their exceptional structural tunability renders them as an ideal platform for advanced therapeutic applications. Herein, two redox-responsive Zn(II)-coordinated porphyrin COF hydrogels are constructed, which demonstrate rapid blood glucose reduction in localized tissues, along with improved angiogenesis, reactive oxygen species (ROS) scavenging, and photothermal antimicrobial capacities within the hyperglycemic blood environment of diabetic patients, thereby effectively controlling infections and concurrently promoting wound healing. Specifically, COFs with built-in dual active sites, i.e., disulfide or diselenide moieties, can be cleaved by ROS, releasing Zn(II) ions that possess antibacterial and tissue-repairing properties. Furthermore, the Zn(II)-porphyrin COF exhibits glucose oxidase (GOX)-like activity, catalyzing the conversion of glucose into non-glucose metabolites. This synergistic combination of glucose-responsive Zn(II) release and GOX-like activities effectively restores tissue redox balance and improves the wound microenvironment, offering a promising strategy for the diagnosis and treatment of diabetic wounds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2404076"},"PeriodicalIF":10.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial DNA Profiles of Bacterial Extracellular Vesicles from 3D Salivary Polymicrobial Biofilms - A Pilot Study. 三维唾液多微生物生物膜细菌胞外囊泡的微生物DNA谱-一项初步研究。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2025-01-02 DOI: 10.1002/adhm.202403300

Chun Liu, Nadeeka S Udawatte, Andrew Liaw, Reuben Staples, Carlos Salomon, Chaminda Jayampath Seneviratne, Sašo Ivanovski, Pingping Han

{"title":"Microbial DNA Profiles of Bacterial Extracellular Vesicles from 3D Salivary Polymicrobial Biofilms - A Pilot Study.","authors":"Chun Liu, Nadeeka S Udawatte, Andrew Liaw, Reuben Staples, Carlos Salomon, Chaminda Jayampath Seneviratne, Sašo Ivanovski, Pingping Han","doi":"10.1002/adhm.202403300","DOIUrl":"https://doi.org/10.1002/adhm.202403300","url":null,"abstract":"With the advent of multi-layered and 3D scaffolds, the understanding of microbiome composition and pathogenic mechanisms within polymicrobial biofilms is continuously evolving. A fundamental component in mediating the microenvironment and bacterial-host communication within the biofilm are bilayered nanoparticles secreted by bacteria, known as bacterial extracellular vesicles (BEVs), which transport key biomolecules including proteins, nucleic acids, and metabolites. Their characteristics and microbiome profiles are yet to be explored in the context of in vitro salivary polymicrobial biofilm. This pilot study aimed to compare the profiles of BEVs from salivary biofilm cultured on a 2D tissue culture plate and 3D melt electrowritten medical-grade polycaprolactone (MEW mPCL) scaffold. BEVs derived from MEW mPCL biofilm exhibited enhanced purity and yield without altered EV morphology and lipopolysaccharide (LPS) content, with enriched BEVs-associated DNA from Capnocytophaga, porphyromonas, and veillonella genus. Moreover, compared to saliva controls, MEW mPCL BEVs showed comparable DNA expression of Tannerella forsythia, and Treponema denticola and significantly higher expression in Porphyromonas gingivalis, Eikenella corrodens and Lactobacillus acidophilus. Together, these findings highlight a more detailed microbial profile with BEVs derived from salivary biofilms cultured on 3D MEW PCL scaffolds, which facilitates an effective in vitro model with a greater resemblance to naturally occurring biofilms.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403300"},"PeriodicalIF":10.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0