Advanced Healthcare Materials最新文献_第7页

Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis. 壳聚糖改性水凝胶微球包裹掺锌生物活性玻璃，通过抑制炎症和促进血管生成修复脊髓损伤

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-09 DOI: 10.1002/adhm.202402129

Xinjin Su, Changjiang Gu, Ziheng Wei, Yanqing Sun, Chao Zhu, Xiongsheng Chen

{"title":"Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis.","authors":"Xinjin Su, Changjiang Gu, Ziheng Wei, Yanqing Sun, Chao Zhu, Xiongsheng Chen","doi":"10.1002/adhm.202402129","DOIUrl":"https://doi.org/10.1002/adhm.202402129","url":null,"abstract":"Spinal cord injury (SCI) is a common nerve injury caused by external force, resulting in sensory and motor impairments. Previous studies demonstrated that inhibiting the neuroinflammation promoted SCI repair. However, these approaches are low efficient, and lack targeting specificity, and even require repeated and high doses of systemic administration. To address such issues, in the present study, chitosan-modified hydrogel microspheres encapsulating with zinc-doped bioactive glasses (CS-MG@Zn/BGs) is constructed for targeted repair of SCI. In vitro, the CS-MG@Zn/BGs effectively inhibited the acute inflammatory response initiated by microglia and promoted angiogenic activities. In vivo, CS-MG@Zn/BGs targeted the injured site, and attenuated neuroinflammation by inhibiting microglia infiltration and modulating microglia polarization toward M2 type. Furthermore, it facilitated vascular reconstruction, neuronal differentiation, axonal regeneration and remyelination at the injured site, and thereby promoted motor function recovery of SCI mice. The in vitro and in vivo results implied that CS-MG@Zn/BGs may be a promising alternative for the rehabilitation of SCI.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402129"},"PeriodicalIF":10.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ligand-Tethered Extracellular Vesicles Mediated RNA Therapy for Liver Fibrosis. 配体系留细胞外囊泡介导的 RNA 治疗肝纤维化。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-09 DOI: 10.1002/adhm.202403068

Yue Liu, Shang Chen, Haoyan Huang, Adam C Midgley, Zhibo Han, Zhong-Chao Han, Qiong Li, Zongjin Li

{"title":"Ligand-Tethered Extracellular Vesicles Mediated RNA Therapy for Liver Fibrosis.","authors":"Yue Liu, Shang Chen, Haoyan Huang, Adam C Midgley, Zhibo Han, Zhong-Chao Han, Qiong Li, Zongjin Li","doi":"10.1002/adhm.202403068","DOIUrl":"https://doi.org/10.1002/adhm.202403068","url":null,"abstract":"Liver fibrosis poses a significant global health burden, in which hepatic stellate cells (HSCs) play a crucial role. Targeted nanomedicine delivery systems directed at HSCs have shown immense potential in the treatment of liver fibrosis. Herein, a bioinspired material, engineered therapeutic miR-181a-5p (a miRNA known to inhibit fibrotic signaling pathways) and targeted moiety hyaluronic acid (HA) co-functionalized extracellular vesicles (EVs) are developed. HA is incorporated onto the surface of EVs using DSPE-PEG as a linker, allowing preferential binding to CD44 receptors, which are overexpressed on activated HSCs. Our results confirmed enhanced cellular uptake and improved payload delivery, as evidenced by the increased intracellular abundance of miR-181a-5p in activated HSCs and fibrotic livers. HA-equipped EVs loaded with miR-181a-5p (DPH-EVs@miR) significantly reduce HSC activation and extracellular matrix (ECM) deposition by inhibiting the TGF-β/Smad signaling pathway, thus alleviating the progression of liver fibrosis. Additionally, DPH-EVs@miR improves liver function, ameliorates inflammatory infiltration, and mitigates hepatocyte apoptosis, demonstrating superior hepatic protective effects. Collectively, this study reports a prospective nanovesicle therapeutic platform loaded with therapeutic miRNA and targeting motifs for liver fibrosis. The biomarker-guided EV-engineering technology utilized in this study provides a promising tool for nanomedicine and precision medicine.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403068"},"PeriodicalIF":10.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioadhesive First-Aid Patch with Rapid Hemostasis and High Toughness Designed for Sutureless Sealing of Acute Bleeding Wounds. 具有快速止血和高韧性的生物粘性急救贴，用于急性出血伤口的无缝合封口。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-09 DOI: 10.1002/adhm.202403412

Ziyuan Zeng, Jiaming Zhang, Yige Gao, Yuanyuan Song, Luoming Liu, Miaomiao Zhu, Wenjing Ma, Jiajun Fu, Dongyang Miao, Chaobo Huang, Ranhua Xiong

{"title":"Bioadhesive First-Aid Patch with Rapid Hemostasis and High Toughness Designed for Sutureless Sealing of Acute Bleeding Wounds.","authors":"Ziyuan Zeng, Jiaming Zhang, Yige Gao, Yuanyuan Song, Luoming Liu, Miaomiao Zhu, Wenjing Ma, Jiajun Fu, Dongyang Miao, Chaobo Huang, Ranhua Xiong","doi":"10.1002/adhm.202403412","DOIUrl":"https://doi.org/10.1002/adhm.202403412","url":null,"abstract":"The global military and civilian sectors express widespread concern over the significant hemorrhage associated with various acute wounds. Such bleedings lead to numerous casualties in military confrontations, traffic accidents, and surgical injuries. Consequently, the rapid control of the bleedings, particularly for extensive and pressurized wounds, is crucial in first-aid situations. In this work, a double-layered bioadhesive patch that combines a superabsorbent adhesive hydrogel with a highly tough antibacterial polyurethane film, which is called as Bio-Patch, is proposed. The Bio-Patch demonstrates superior mechanical strength and forms robust bioadhesion to acute bleeding wounds. Furthermore, the Bio-Patch enables protecting against external Gram-negative and Gram-positive bacteria. Thanks to the double-layered structures having synergistic functions of stable barrier and robust adhesion, the Bio-Patch provides optimal wound sealing (burst strength exceeding 310 mmHg) both in vitro and in vivo. It also demonstrates superior hemostatic effects (less than 30 s) in vivo. This offers promising opportunities for rapid control of extensive and pressurized hemorrhage in first-aid clinical scenarios.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403412"},"PeriodicalIF":10.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECM Proteins Nidogen-1 and Decorin Restore Functionality of Human Islets of Langerhans upon Hypoxic Conditions. ECM 蛋白质 Nidogen-1 和 Decorin 可在缺氧条件下恢复人类朗格汉斯胰岛的功能。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-07 DOI: 10.1002/adhm.202403017

Abiramy Jeyagaran, Max Urbanczyk, Daniel Carvajal-Berrio, Teresa Baldissera, Philipp D Kaiser, Laurence Kuhlburger, Stefan Czemmel, Sven Nahnsen, Garry P Duffy, Sara Y Brucker, Shannon L Layland, Katja Schenke-Layland

{"title":"ECM Proteins Nidogen-1 and Decorin Restore Functionality of Human Islets of Langerhans upon Hypoxic Conditions.","authors":"Abiramy Jeyagaran, Max Urbanczyk, Daniel Carvajal-Berrio, Teresa Baldissera, Philipp D Kaiser, Laurence Kuhlburger, Stefan Czemmel, Sven Nahnsen, Garry P Duffy, Sara Y Brucker, Shannon L Layland, Katja Schenke-Layland","doi":"10.1002/adhm.202403017","DOIUrl":"https://doi.org/10.1002/adhm.202403017","url":null,"abstract":"Transplantation of donor islets of Langerhans is a potential therapeutic approach for patients with diabetes mellitus; however, its success is limited by islet death and dysfunction during the initial hypoxic conditions at the transplantation site. This highlights the need to support the donor islets in the days post-transplantation until the site is vascularized. It was previously demonstrated that the extracellular matrix (ECM) proteins nidogen-1 (NID1) and decorin (DCN) improve the functionality and survival of the β-cell line, EndoC-βH3, and the viability of human islets post-isolation. To advance the use of these ECM proteins toward a clinical application and elucidate the mechanisms of action in primary islets, the study assesses the effects of ECM proteins NID1 and DCN on isolated human donor islets cultured in normoxic and hypoxic conditions. NID1- and DCN-treatment restore β-cell functionality of human donor islets in a hypoxic environment through upregulation of genes involved in glycolytic pathways and reducing DNA fragmentation in hypoxic conditions comparable to normoxic control islets. The results demonstrate that the utilization of NID1 or DCN with islets of Langerhans may have the potential to overcome the hypoxia-induced cell death observed post-transplantation and improve transplant outcomes.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403017"},"PeriodicalIF":10.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermoforming for Small Feature Replication in Melt Electrowritten Membranes to Model Kidney Proximal Tubule. 在熔融电泳膜中进行小特征复制的热成型技术，以模拟肾近曲小管。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-07 DOI: 10.1002/adhm.202401800

Marta G Valverde, Claudia Stampa Zamorano, Dora Kožinec, Laura Benito Zarza, Anne Metje van Genderen, Robine Janssen, Miguel Castilho, Andrei Hrynevich, Tina Vermonden, Jos Malda, Mylene de Ruijter, Rosalinde Masereeuw, Silvia M Mihăilă

{"title":"Thermoforming for Small Feature Replication in Melt Electrowritten Membranes to Model Kidney Proximal Tubule.","authors":"Marta G Valverde, Claudia Stampa Zamorano, Dora Kožinec, Laura Benito Zarza, Anne Metje van Genderen, Robine Janssen, Miguel Castilho, Andrei Hrynevich, Tina Vermonden, Jos Malda, Mylene de Ruijter, Rosalinde Masereeuw, Silvia M Mihăilă","doi":"10.1002/adhm.202401800","DOIUrl":"https://doi.org/10.1002/adhm.202401800","url":null,"abstract":"A novel approach merging melt electrowriting (MEW) with matched die thermoforming to achieve scaffolds with micron-sized curvatures (200 - 800 µm versus 1000 µm of mandrel printing) for in vitro modeling of the kidney proximal tubule (PT) is proposed. Recent advances in this field emphasize the relevance of accurately replicating the intricate tissue microenvironment, particularly the curvature of the nephrons' tubular segments. While MEW offers promising capabilities for fabricating highly and porous precise 3D structures mimicking the PT, challenges persist in approximating the diameter of tubular scaffolds to match the actual PT. The thermoformed MEW membranes retain the initial MEW printing design parameters (rhombus geometry, porosity > 45%) while accurately following the imprinted curvature (ratios between 0.67-0.95). PT epithelial cells cultured on these membranes demonstrate the ability to fill in the large pores of the membrane by secreting their own collagen IV-rich extracellular matrix and form an organized, functional, and tight monolayer expressing characteristic PT markers. Besides approximating PT architecture, this setup maximizes the usable surface area for cell culture and molecular readouts. By closely mimicking the structural intricacies of native tissue architecture, this approach enhances the biomimetic fidelity of engineered scaffolds, offering potential applications beyond kidney tissue engineering.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2401800"},"PeriodicalIF":10.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Forming Supramolecular Nanofiber Hydrogel as a Biodegradable Liquid Embolic Agent for Postembolization Tissue Remodeling. 原位形成超分子纳米纤维水凝胶作为栓塞后组织重塑的可生物降解液体栓塞剂

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-07 DOI: 10.1002/adhm.202403784

Akihiro Nishiguchi, Miho Ohta, Debabrata Palai, Shima Ito, Kensaku Mori, Ryotaro Akagi, Christophe Bajan, Guillaume Lambard, Keitaro Sodeyama, Tetsushi Taguchi

{"title":"In Situ Forming Supramolecular Nanofiber Hydrogel as a Biodegradable Liquid Embolic Agent for Postembolization Tissue Remodeling.","authors":"Akihiro Nishiguchi, Miho Ohta, Debabrata Palai, Shima Ito, Kensaku Mori, Ryotaro Akagi, Christophe Bajan, Guillaume Lambard, Keitaro Sodeyama, Tetsushi Taguchi","doi":"10.1002/adhm.202403784","DOIUrl":"https://doi.org/10.1002/adhm.202403784","url":null,"abstract":"Embolic agents have been widely used to treat blood vessel abnormalities in interventional radiology as a minimally invasive procedure. However, only a few biodegradable liquid embolic agents exhibit high embolization performance, biodegradability, and operability. Herein, the design of in situ-forming supramolecular nanofiber (SNF) hydrogels is reported as biodegradable liquid embolic agents with the assistance of Bayesian optimization through an active learning pipeline. Chemically modified gelatin with hydrogen-bonding moieties produces fibrin-inspired nanofiber-based hydrogels with a high blood coagulation capacity. The low viscosity of the SNF hydrogels makes them injectable using a microcatheter, and the hydrogel shows sufficient tissue adhesion to the blood vessel walls and very weak adhesion to the catheter tubes. Moreover, the SNF hydrogels exhibit high blood compatibility, cytocompatibility, cell-adhesive properties, and biodegradability (in vitro and in vivo). Intravascularly delivered SNF hydrogels induce embolization of rat femoral arteries. This biodegradable liquid embolic agent could be a powerful tool for interventional radiology in the treatment of various diseases, including aortic aneurysm stent grafting, gynecological diseases, and liver cancer.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403784"},"PeriodicalIF":10.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Cardiac Tissues as a Platform for CRISPR-Based Mitogen Discovery. 将人造心脏组织作为基于 CRISPR 的丝裂原发现平台

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-07 DOI: 10.1002/adhm.202402201

Sophia DeLuca, Nicholas Strash, Yifan Chen, Marisa Patsy, Ashley Myers, Libertad Tejeda, Sarah Broders, Amber Miranda, Xixian Jiang, Nenad Bursac

{"title":"Engineered Cardiac Tissues as a Platform for CRISPR-Based Mitogen Discovery.","authors":"Sophia DeLuca, Nicholas Strash, Yifan Chen, Marisa Patsy, Ashley Myers, Libertad Tejeda, Sarah Broders, Amber Miranda, Xixian Jiang, Nenad Bursac","doi":"10.1002/adhm.202402201","DOIUrl":"10.1002/adhm.202402201","url":null,"abstract":"Improved understanding of cardiomyocyte (CM) cell cycle regulation may allow researchers to stimulate pro-regenerative effects in injured hearts or promote maturation of human stem cell-derived CMs. Gene therapies, in particular, hold promise to induce controlled proliferation of endogenous or transplanted CMs via transient activation of mitogenic processes. Methods to identify and characterize candidate cardiac mitogens in vitro can accelerate translational efforts and contribute to the understanding of the complex regulatory landscape of CM proliferation and postnatal maturation. In this study, A CRISPR knockout-based screening strategy using in vitro neonatal rat ventricular myocyte (NRVM) monolayers is established, followed by candidate mitogen validation in mature 3-D engineered cardiac tissues (ECTs). This screen identified knockout of the purine metabolism enzyme adenosine deaminase (ADA-KO) as an effective pro-mitogenic stimulus. RNA-sequencing of ECTs further reveals increased pentose phosphate pathway (PPP) activity as the primary driver of ADA-KO-induced CM cycling. Inhibition of the pathway's rate limiting enzyme, glucose-6-phosphate dehydrogenase (G6PD), prevented ADA-KO induced CM cycling, while increasing PPP activity via G6PD overexpression increased CM cycling. Together, this study demonstrates the development and application of a genetic/tissue engineering platform for in vitro discovery and validation of new candidate mitogens affecting regenerative or maturation states of cardiomyocytes.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402201"},"PeriodicalIF":10.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthracene-Based Endoperoxides as Self-Sensitized Singlet Oxygen Carriers for Hypoxic-Tumor Photodynamic Therapy. 蒽类内过氧化物作为自敏化单线态氧载体用于缺氧-肿瘤光动力疗法

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-06 DOI: 10.1002/adhm.202403009

Yan-Qin He, Jian-Hong Tang

{"title":"Anthracene-Based Endoperoxides as Self-Sensitized Singlet Oxygen Carriers for Hypoxic-Tumor Photodynamic Therapy.","authors":"Yan-Qin He, Jian-Hong Tang","doi":"10.1002/adhm.202403009","DOIUrl":"10.1002/adhm.202403009","url":null,"abstract":"Singlet oxygen is a crucial reactive oxygen species (ROS) in photodynamic therapy (PDT). However, the hypoxic tumor microenvironment limits the production of cytotoxic singlet oxygen through the light irradiation of PDT photosensitizers (PSs). This restriction poses a major challenge in improving the effectiveness of PDT. To overcome this challenge, researchers have explored the development of singlet oxygen carriers that can capture and release singlet oxygen in physiological conditions. Among these developments, anthracene-based endoperoxides, initially discovered almost 100 years ago, have shown the ability to generate singlet oxygen controllably under thermal or photo stimuli. Recent advancements have led to the development of a new class of self-sensitized anthracene-endoperoxides, with potential applications in enhancing PDT effects for hypoxic tumors. This review discusses the current research progress in utilizing self-sensitized anthracene-endoperoxides as singlet oxygen carriers for improved PDT. It covers anthracene-conjugated small organic molecules, metal-organic complexes, polymeric structures, and other self-sensitized nano-structures. The molecular structural designs, mechanisms, and characteristics of these systems will be discussed. This review aims to provide valuable insights for developing high-performance singlet oxygen carriers for hypoxic-tumor PDT.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403009"},"PeriodicalIF":10.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpenetrating Polymer Network Hydrogels with Tunable Viscoelasticity and Proteolytic Cleavability to Direct Stem Cells In Vitro. 具有可调粘弹性和蛋白水解裂解性的互穿聚合物网络水凝胶可引导体外干细胞。

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-06 DOI: 10.1002/adhm.202402656

Prannoy Seth, Jens Friedrichs, Yanuar Dwi Putra Limasale, Nicole Fertala, Uwe Freudenberg, Yixin Zhang, Ayala Lampel, Carsten Werner

{"title":"Interpenetrating Polymer Network Hydrogels with Tunable Viscoelasticity and Proteolytic Cleavability to Direct Stem Cells In Vitro.","authors":"Prannoy Seth, Jens Friedrichs, Yanuar Dwi Putra Limasale, Nicole Fertala, Uwe Freudenberg, Yixin Zhang, Ayala Lampel, Carsten Werner","doi":"10.1002/adhm.202402656","DOIUrl":"10.1002/adhm.202402656","url":null,"abstract":"The dynamic nature of cellular microenvironments, regulated by the viscoelasticity and enzymatic cleavage of the extracellular matrix, remains challenging to emulate in engineered synthetic biomaterials. To address this, a novel platform of cell-instructive hydrogels is introduced, composed of two concurrently forming interpenetrating polymer networks (IPNs). These IPNs consist of the same basic building blocks - four-armed poly(ethylene glycol) and the sulfated glycosaminoglycan (sGAG) heparin - are cross-linked through either chemical or physical interactions, allowing for precise and selective tuning of the hydrogel's stiffness, viscoelasticity, and proteolytic cleavability. The studies of the individual and combined effects of these parameters on stem cell behavior revealed that human mesenchymal stem cells exhibited increased spreading and Yes-associated protein transcriptional activity in more viscoelastic and cleavable sGAG-IPN hydrogels. Furthermore, human induced pluripotent stem cell (iPSC) cysts displayed enhanced lumen formation, growth, and pluripotency maintenance when cultured in sGAG-IPN hydrogels with higher viscoelasticity. Inhibition studies emphasized the pivotal roles of actin dynamics and matrix metalloproteinase activity in iPSC cyst morphology, which varied with the viscoelastic properties of the hydrogels. Thus, the introduced sGAG-IPN hydrogel platform offers a powerful methodology for exogenous stem cell fate control.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2402656"},"PeriodicalIF":10.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composite Scaffold Materials of Nanocerium Oxide Doped with Allograft Bone: Dual Optimization Based on Anti-Inflammatory Properties and Promotion of Osteogenic Mineralization. 掺杂同种异体骨的纳米氧化铈复合支架材料：基于抗炎特性和促进成骨矿化的双重优化

IF 1 2区医学

Advanced Healthcare Materials Pub Date : 2024-11-06 DOI: 10.1002/adhm.202403006

Yuqiao Li, Yongkang Huang, Houzhi Yang, Yuxin Li, Gan Luo, Yuan Ma, Haiyang Cheng, Yubin Long, Weiwei Xia, Haiying Liu, Huayi Fang, Yaping Du, Tianwei Sun, Kaifeng Wang, Xinyun Zhai

{"title":"Composite Scaffold Materials of Nanocerium Oxide Doped with Allograft Bone: Dual Optimization Based on Anti-Inflammatory Properties and Promotion of Osteogenic Mineralization.","authors":"Yuqiao Li, Yongkang Huang, Houzhi Yang, Yuxin Li, Gan Luo, Yuan Ma, Haiyang Cheng, Yubin Long, Weiwei Xia, Haiying Liu, Huayi Fang, Yaping Du, Tianwei Sun, Kaifeng Wang, Xinyun Zhai","doi":"10.1002/adhm.202403006","DOIUrl":"https://doi.org/10.1002/adhm.202403006","url":null,"abstract":"Spinal fusion technique is widely used in the treatment of lumbar degeneration, cervical instability, disc injury, and spinal deformity. However, it is usually accompanied by a high incidence of fusion failure and pseudoarthrosis, placing higher demands on bone implants. Therefore, materials with good biocompatibility, osteoconductivity, and even induce bone ingrowth, which can be used to improve spinal fusion rate and bone regeneration, have become a hot research topic. Here, ultra-small cerium oxide nanoparticles (CeO2 NPs) are prepared and loaded onto the surface of the homograft bone surface to prepare a composite scaffold AB@PLGA/CeO2. The composite scaffold shows the competitive ability to promote osteoblast differentiation in vitro. In vivo experiments show that AB@PLGA/CeO2 has a good bone enhancement effect. In particular, good biological effects of collagen fiber formation, osteogenic mineralization, and tissue repair are shown in intervertebral implant fusion. Further, transcriptome sequencing confirms that CeO2 NPs promote osteogenic differentiation and mineralization by regulating extracellular matrix (ECM) and collagen formation. Meanwhile, CeO2 NPs can regulate the function of the PI3K-Akt signaling pathway to exert its ability to promote osteogenic differentiation and mineralization and affect p53 and cell cycle signaling pathway to regulate osteogenic differentiation and mineralization. Hence, the proposed scaffold is a promising strategy for intervertebral fusion in the clinic.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e2403006"},"PeriodicalIF":10.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0