Advanced Healthcare Materials最新文献_第5页

Growth Hormone-Loaded 3D Printed Silk Fibroin-Cellulose Dressings for Ischemic Wounds. 承载生长激素的3D打印丝素纤维素敷料用于缺血性伤口。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-14 DOI: 10.1002/adhm.202502969

Maria Pita-Vilar, Diego Caicedo-Valdés, Susana B Bravo, Isabel Rodriguez-Moldes, Salvador D Aznar-Cervantes, Angel Concheiro, Carmen Alvarez-Lorenzo, Luis Diaz-Gomez

{"title":"Growth Hormone-Loaded 3D Printed Silk Fibroin-Cellulose Dressings for Ischemic Wounds.","authors":"Maria Pita-Vilar, Diego Caicedo-Valdés, Susana B Bravo, Isabel Rodriguez-Moldes, Salvador D Aznar-Cervantes, Angel Concheiro, Carmen Alvarez-Lorenzo, Luis Diaz-Gomez","doi":"10.1002/adhm.202502969","DOIUrl":"https://doi.org/10.1002/adhm.202502969","url":null,"abstract":"In this study, carboxymethyl cellulose-silk fibroin (CMC-SF) wound dressings loaded with growth hormone (GH) are developed for chronic wound care, particularly in diabetic ulcer regeneration. Designed with a porous and reproducible structure, the dressings enable rapid, localized GH release within 24 h and maintain high fluid absorption with minimal swelling, ensuring optimal conformity to the wound site. The inclusion of SF and GH significantly enhances cell proliferation, migration, and angiogenesis in vitro. The dressings also enable nontraumatic removal, as demonstrated in an in ovo model, supporting their suitability for clinical applications. In vivo testing in ischemic diabetic wounds shows accelerated tissue regeneration, reduced scarring, and improved healing quality. Proteomics and immunohistochemical analyses indicate that GH contributes to a more balanced inflammatory response, enhanced antioxidant activity and vascularization, and better regulation of tissue remodeling processes. Overall, the incorporation of GH within the CMC-SF dressings represents a promising and effective approach to support diabetic ulcer regeneration.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e02969"},"PeriodicalIF":9.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable Dual-Crosslinked Poly(oligo(Ethylene Glycol) Methacrylate) Hydrogels Inspired by Mussel Adhesion for Cutaneous Wound Healing and Functional Tissue Regeneration. 可注射的双交联聚（低聚乙二醇）甲基丙烯酸酯水凝胶，灵感来自贻贝粘连，用于皮肤伤口愈合和功能性组织再生。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-14 DOI: 10.1002/adhm.202502866

Gurpreet Kaur Randhawa, Meghan Kostashuk, Zhicheng Pan, Nils Nordstrom, Thomas Kalab, Erica Winterhelt, Todd Hoare

{"title":"Injectable Dual-Crosslinked Poly(oligo(Ethylene Glycol) Methacrylate) Hydrogels Inspired by Mussel Adhesion for Cutaneous Wound Healing and Functional Tissue Regeneration.","authors":"Gurpreet Kaur Randhawa, Meghan Kostashuk, Zhicheng Pan, Nils Nordstrom, Thomas Kalab, Erica Winterhelt, Todd Hoare","doi":"10.1002/adhm.202502866","DOIUrl":"https://doi.org/10.1002/adhm.202502866","url":null,"abstract":"Traditional dermal wound closure methods, such as sutures and staples, are invasive, causing soft tissue trauma, increasing the likelihood of inflammation and infections. Alternatively, while existing tissue adhesives can seal and adhere to wounds, they may cause immunogenic responses, tissue necrosis, restricted movement, and wound disruption upon removal, leading to secondary injuries and scarring. Herein, injectable poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA)-dopamine (DA) hydrogels co-crosslinked via hydrazone linkages and dopamine self-polymerization are fabricated that promote high water retention, effective tissue adhesion, re-epithelialization, and functional skin regeneration. The dual crosslinking mechanism allows for gelation as fast as 24 s (enabling injection and rapid filling of irregularly-shaped wounds) while achieving compressive moduli of up to 37 kPa and skin adhesion strengths of up to 3.3 kPa. In a 14-day stented mouse skin wound model, the POEGMA-DA hydrogels induce no significant inflammation, effective tissue adhesion, and promote tissue regeneration, including enhanced collagen remodelling, 3-5× higher hair follicle, ≈5-7× higher sebaceous gland and 1.5-1.7× higher blood vessel regeneration at the excision site compared to untreated wounds. These hydrogels represent an alternative nontoxic wound closure system that mimics the soft skin tissue environment to promote regeneration after acute superficial dermal wounds.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e02866"},"PeriodicalIF":9.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strain-Sensitive and Strain-Insensitive Flexible Electronics for Healthcare Monitoring. 应变敏感和应变不敏感柔性电子保健监测。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-14 DOI: 10.1002/adhm.202503333

Zheming Zhang, Yimeng Xu, Zijie Zhang, Shan Zhang, Jiankang Liu, Pengfei Zhang, Siguo Sun, Mingrui Wang, Ziyi Dai, Kai Qian

{"title":"Strain-Sensitive and Strain-Insensitive Flexible Electronics for Healthcare Monitoring.","authors":"Zheming Zhang, Yimeng Xu, Zijie Zhang, Shan Zhang, Jiankang Liu, Pengfei Zhang, Siguo Sun, Mingrui Wang, Ziyi Dai, Kai Qian","doi":"10.1002/adhm.202503333","DOIUrl":"https://doi.org/10.1002/adhm.202503333","url":null,"abstract":"Flexible electronics have emerged as an important technology in healthcare monitoring, enabling continuous assessment of physiological signals through conformable integration with human tissues. The mechanical deformation of human tissues during daily activities presents a unique challenge: some monitoring applications require high strain sensitivity for accurate motion detection, while others demand stable electrical performance regardless of mechanical deformation. This review systematically examines recent advances in flexible electronics for healthcare monitoring, classifying devices based on their fundamental design objective, whether to detect or to ignore mechanical strain. The fundamental mechanisms and material strategies for achieving controllable strain response in both strain-sensitive and strain-insensitive designs are first respectively analyzed. Subsequently, physiological monitoring requirements are mapped across anatomical systems from cavity organs to hard tissues, demonstrating how different strain environments necessitate specific device design strategies. It is further explored how these strain-engineered properties enable various monitoring functions, from motion tracking and rehabilitation assessment to continuous vital sign monitoring and chemical sensing. Finally, current challenges in stability and biocompatibility are addressed, while perspectives on future developments in material design and device integration are provided. Through this strain-magnitude-based systematic examination, this review aims to facilitate the rational design of flexible electronic devices for specific healthcare monitoring needs.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03333"},"PeriodicalIF":9.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Printing of Bacteriophage-Loaded Hydrogels: Development of a Local and Long-Lasting Delivery System. 噬菌体负载水凝胶的3D打印：局部和持久的输送系统的发展。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-13 DOI: 10.1002/adhm.202503113

Corina Vater, Gopala Krishna Mannala, Max von Witzleben, Richard Frank Richter, Nike Walter, Michael Gelinsky, Volker Alt, Anja Lode, Markus Rupp

{"title":"3D Printing of Bacteriophage-Loaded Hydrogels: Development of a Local and Long-Lasting Delivery System.","authors":"Corina Vater, Gopala Krishna Mannala, Max von Witzleben, Richard Frank Richter, Nike Walter, Michael Gelinsky, Volker Alt, Anja Lode, Markus Rupp","doi":"10.1002/adhm.202503113","DOIUrl":"https://doi.org/10.1002/adhm.202503113","url":null,"abstract":"Multiple drug-resistant bacteria are a growing life-threatening problem and novel treatment strategies are urgently needed. One promising option is the use of lytic bacteriophages, viruses that infect and kill bacteria with high specificity. To efficiently utilize bacteriophage therapy for the treatment of implant-associated infections, an effective strategy for the local, long-lasting administration of bacteriophages at the site of infection is required. With the aim of developing a defined delivery system, this study investigates the feasibility of 3D extrusion printing of bacteriophages embedded in biomaterial inks by using a Staphylococcus aureus-specific phage strain as model. It is demonstrated that a bacteriophage-loaded hydrogel blend consisting of alginate and methylcellulose (AlgMC) can be printed with high shape fidelity. After cross-linking, the hydrogel constructs release bacteriophages that maintain their activity against S. aureus over a period of 35 days when incubated in human-plasma-like medium (HPLM). The integration of the nanoclay Laponite into the AlgMC blend, known for its high binding capacity for biomolecules, does not further prolong the release under (near) physiological conditions in HPLM but may protect bacteriophages under nonphysiological conditions. In conclusion, bacteriophage-loaded AlgMC inks fulfill the requirements for local bacteriophage therapy as they release active bacteriophages in a sustained manner.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03113"},"PeriodicalIF":9.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized Extracellular Matrix Scaffolds to Engineer the Dormant Landscape of Microscopic Colorectal Cancer Liver Metastasis. 脱细胞细胞外基质支架构建结直肠癌肝转移的休眠景观。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-13 DOI: 10.1002/adhm.202501791

Sabrina N VandenHeuvel, Lucia L Nash, Abigail J Clevenger, Claudia A Collier, Oscar R Benavides, Sanjana Roy, Brinlee Goggans, Aelita Salikhova, Anvitha Tharakesh, Svasti Haricharan, Amber N Stratman, Scott Kopetz, Alex J Walsh, Shreya A Raghavan

{"title":"Decellularized Extracellular Matrix Scaffolds to Engineer the Dormant Landscape of Microscopic Colorectal Cancer Liver Metastasis.","authors":"Sabrina N VandenHeuvel, Lucia L Nash, Abigail J Clevenger, Claudia A Collier, Oscar R Benavides, Sanjana Roy, Brinlee Goggans, Aelita Salikhova, Anvitha Tharakesh, Svasti Haricharan, Amber N Stratman, Scott Kopetz, Alex J Walsh, Shreya A Raghavan","doi":"10.1002/adhm.202501791","DOIUrl":"https://doi.org/10.1002/adhm.202501791","url":null,"abstract":"Recurrent liver-metastatic colorectal cancer contributes to high mortality. Recurrence occurs when dormant, microscopic residual disease survives initial treatment to escape dormancy. In their dormant, microscopic state within the liver, these metastatic lesions are undetectable by clinical diagnostic imaging until they form overt, chemoresistant metastases. Therefore, understanding the molecular mechanisms underlying dormancy in colorectal cancer liver metastases is a significant knowledge gap, motivating the engineering of nuanced in vitro models of disease. The current work presents an engineered model of liver-metastatic colorectal cancer dormancy. Decellularized extracellular matrix (dECM) scaffolds are used to provide microscopic colorectal cancer cell clusters with a biomimetic, 3D liver-specific architecture to colonize. Combined with nutrient deprivation and low dose chemotherapy, liver dECM significantly promotes dormancy, which manifests as slowed proliferation, nutrient/chemo-dependent G1/S and ECM-driven G2/M cell cycle arrest, diminished tumorigenicity, and robust chemotherapy resistance. The engineered dormancy signature is reversible, mimicking dormancy escape. The dECM-based model of engineered dormant colorectal cancer liver metastasis is crucial for advancing knowledge of dormancy induction and reversal, to improve therapeutics and patient survival.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e01791"},"PeriodicalIF":9.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable Biomimetic Lubricant-PRP Hydrogel with Synergistic Lubrication from In/Ex-Sources for Cartilage Regeneration in Rabbits. 具有内/外源协同润滑的可注射仿生润滑剂- prp水凝胶用于兔软骨再生。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-13 DOI: 10.1002/adhm.202503551

Yongan Lin, Zijian Yan, Jiayi Chen, Xiaochao Wang, Ruibin Lin, Yunqi Fu, Zhaoying Lv, Bohui Wu, Xuewei Cao, Ming Dong, Chenxiao Zheng, Li Ren

{"title":"Injectable Biomimetic Lubricant-PRP Hydrogel with Synergistic Lubrication from In/Ex-Sources for Cartilage Regeneration in Rabbits.","authors":"Yongan Lin, Zijian Yan, Jiayi Chen, Xiaochao Wang, Ruibin Lin, Yunqi Fu, Zhaoying Lv, Bohui Wu, Xuewei Cao, Ming Dong, Chenxiao Zheng, Li Ren","doi":"10.1002/adhm.202503551","DOIUrl":"https://doi.org/10.1002/adhm.202503551","url":null,"abstract":"Cartilage regeneration remains a clinical challenge, as existing treatments (including pharmacological and physiological treatments) only alleviate symptoms. This work proposes a novel strategy of in/ex-source synergistic lubrication and develops an injectable bioactive hydrogel that synergistically lubricates articular cartilage and promotes cartilage regenerative repair by providing immediate exogenous biomimetic lubricant and promoting endogenous synovial fluid secretion. The hydrogel, composed of a biomimetic lubricant (HDPA) and platelet-rich-plasma (PRP), rapidly forms a gel via biological cross-linking, offering both physical lubrication and bioremediation functions. It significantly reduces cartilage friction, facilitates cell proliferation, migration or recruitment, and promotes their chondrogenic differentiation. Additionally, it stimulates synovial fibroblasts to secrete endogenous hyaluronic acid, achieving in/ex-source lubrication. RNA-seq reveals suppression of pro-inflammatory NF-κB/IL-1 pathways and activation of TGF-β signaling, promoting hyaluronic acid (HA) synthesis and ECM remodeling. In a rabbit model, the hydrogel induces hyaline cartilage regeneration and achieves the optimal healing effect, outperforming controls and single treatments. The approach of combining a biomimetic lubricant with PRP, therefore, is potentially useful for the treatment of osteoarthritis.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03551"},"PeriodicalIF":9.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Bioprinting and Actuation of Stretchable Muscle Tissue. 磁性生物打印和可拉伸肌肉组织的驱动。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-12 DOI: 10.1002/adhm.202503035

Noam Demri, Lise Morizur, Simon Dumas, Giacomo Gropplero, Cécile Martinat, Stéphanie Descroix, Claire Wilhelm

{"title":"Magnetic Bioprinting and Actuation of Stretchable Muscle Tissue.","authors":"Noam Demri, Lise Morizur, Simon Dumas, Giacomo Gropplero, Cécile Martinat, Stéphanie Descroix, Claire Wilhelm","doi":"10.1002/adhm.202503035","DOIUrl":"https://doi.org/10.1002/adhm.202503035","url":null,"abstract":"Engineering tissues with precise, long-lasting shapes and the capability for mechanical stimulation remains challenging. This study addresses this challenge by developing a next-generation magnetic bioprinting approach to create anisotropic, shape-controlled, scaffold-free, and stretchable skeletal muscle constructs. Murine skeletal muscle cells and human induced pluripotent stem cell-derived skeletal muscle cells, labeled with iron oxide nanoparticles, are magnetically bioprinted into wrench-shaped tissues. Their magnetic properties allow these tissues to be clipped onto magnetic needles, preserving their shape over two weeks of culture while promoting anisotropic differentiation and myoblast fusion. Additionally, the magnetic tissues can be stretched by up to 100%, enhancing their anisotropy and improving muscle maturation. This magnetic toolbox demonstrates significant advancements in muscle tissue engineering, as evidenced by enhanced indicators of myoblast differentiation, including cell fusion, increased myogenic maturation, and contractility. These findings highlight the potential of magnetic-based techniques for developing advanced muscle-on-chip systems and other complex tissue constructs.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03035"},"PeriodicalIF":9.6,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaffold-Guided Subchondral Bone Repair Enables Endogenous Stem Cell-Driven Cartilage Regeneration in Osteochondral Defects. 支架引导软骨下骨修复实现内源性干细胞驱动的骨软骨缺损软骨再生。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-12 DOI: 10.1002/adhm.202502394

Yang Liu, Lei Liu, Jiawei Li, Yuanhao Fan, Zhongyi Chen, Yue Wang, Jianqun Wu, Yu Li, Ziyu Guo, Shenlei Feng, Jiaming Bai, Decheng Wu, Chao Liu

{"title":"Scaffold-Guided Subchondral Bone Repair Enables Endogenous Stem Cell-Driven Cartilage Regeneration in Osteochondral Defects.","authors":"Yang Liu, Lei Liu, Jiawei Li, Yuanhao Fan, Zhongyi Chen, Yue Wang, Jianqun Wu, Yu Li, Ziyu Guo, Shenlei Feng, Jiaming Bai, Decheng Wu, Chao Liu","doi":"10.1002/adhm.202502394","DOIUrl":"https://doi.org/10.1002/adhm.202502394","url":null,"abstract":"Osteochondral defects involve concurrent damage to cartilage and the subchondral bone. Here, a cell-free scaffold is presented, consisting of a 3D-printed bioceramic base combined with a Gelatin methacryloyl (GelMA)-Kartogenin (KGN) hydrogel. This dual induction scaffold is engineered to promote osteogenesis while simultaneously providing localized chondrogenic stimulation. The rabbit bone marrow-derived mesenchymal stromal cells are added as a positive control, while the blank osteochondral defects without scaffold implantation are set as a negative control. It is hypothesized that effective regeneration of subchondral bone is a prerequisite for functional cartilage repair, with effective recruitment of endogenous skeletal stem cells (SSCs). In rabbit osteochondral defects, chondrogenic scaffolds alone regenerated cartilage but caused severe subchondral bone collapse and joint surface deformation persisting through 24 weeks. In contrast, combining osteogenic scaffolds with chondrogenic constructs preserved joint morphology by promoting Gli-1⁺ and Sca-1⁺ skeletal stem cell recruitment and proliferation. Interestingly, adding exogenous mesenchymal stromal cells offered no further benefit. Together, a scaffold capable of recruiting endogenous skeletal stem cells to regenerate subchondral bone is essential for effective osteochondral repair and demonstrates comparable efficacy to stem cell transplantation, demonstrating the viability of a scaffold-only strategy for articular cartilage and subchondral bone tissue regeneration.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e02394"},"PeriodicalIF":9.6,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Site-Specific Antibacterial Strategy for Multi-Pathway Treatment of Drug-Resistant Skin Infections. 多途径治疗耐药皮肤感染的位点特异性抗菌策略。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-11 DOI: 10.1002/adhm.202504240

Lu Tang, Hening Liu, Jingwen Feng, Yi Yao, Yuqi Cao, Yue Yin, Cong Fu, Jifan Gao, Qiaqia Xiao, Ziwei Yan, Weijie Shu, Rou Wen, Mengliang Zhu, Xing-Jie Liang, Wei Wang

{"title":"Site-Specific Antibacterial Strategy for Multi-Pathway Treatment of Drug-Resistant Skin Infections.","authors":"Lu Tang, Hening Liu, Jingwen Feng, Yi Yao, Yuqi Cao, Yue Yin, Cong Fu, Jifan Gao, Qiaqia Xiao, Ziwei Yan, Weijie Shu, Rou Wen, Mengliang Zhu, Xing-Jie Liang, Wei Wang","doi":"10.1002/adhm.202504240","DOIUrl":"https://doi.org/10.1002/adhm.202504240","url":null,"abstract":"Drug-resistant skin infections, especially those caused by multidrug-resistant (MDR) bacteria, remain a major public health concern due to the limited efficacy of conventional antibiotics and biofilm-associated tolerance. Herein, a site-specific antibacterial strategy based on a multi-pathway microneedle (MN) patch system is presented for effective treatment of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds and abscesses. The MN patch co-delivers vancomycin and photoactive black phosphorus quantum dots (BPQDs) encapsulated in macrophage membrane-coated cationic liposomes, thereby integrating antibiotic therapy with phototherapy. Upon light activation, BPQDs generate localized hyperthermia and reactive oxygen species, which synergize with vancomycin to eradicate bacteria and reduce the risk of resistance development. The dissolvable MN array ensures efficient penetration through the skin barrier, enabling targeted and sustained release at the infection site. In vivo, this multi-pathway intervention significantly accelerates wound closure, reduces abscess size, suppresses inflammation, and promotes tissue regeneration by remodeling the infectious microenvironment. Overall, this work demonstrates a promising localized therapeutic platform that harnesses multi-pathway antibacterial mechanisms to combat MDR bacteria and facilitate the healing of drug-resistant skin infections.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e04240"},"PeriodicalIF":9.6,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Neuro-Regenerative Peptide Hydrogel for Directed Neural Lineage Reprograming and Regeneration of Sciatic Nerve Injury. 用于坐骨神经损伤定向神经系重编程和再生的工程神经再生肽水凝胶。

IF 9.6 2区医学

Advanced Healthcare Materials Pub Date : 2025-10-10 DOI: 10.1002/adhm.202503429

Shubham Garg, Aniket Jana, Sanju Gupta, Mohammad Umar Arshi, Juhee Khan, Prabir Gharai, Rajsekhar Roy, Surajit Ghosh

{"title":"Engineered Neuro-Regenerative Peptide Hydrogel for Directed Neural Lineage Reprograming and Regeneration of Sciatic Nerve Injury.","authors":"Shubham Garg, Aniket Jana, Sanju Gupta, Mohammad Umar Arshi, Juhee Khan, Prabir Gharai, Rajsekhar Roy, Surajit Ghosh","doi":"10.1002/adhm.202503429","DOIUrl":"https://doi.org/10.1002/adhm.202503429","url":null,"abstract":"Living systems are distinguished by their ability to self-organization, a phenomenon that drives a range of pattern-forming processes, from sand dunes to cellular assemblies and tissue architectures. Inspired by these natural phenomena and design principles, the fabrication of an engineered nanofibrous, extracellular matrix (ECM) mimicking self-assembling peptide hydrogel is reported. This hydrogel integrates a neuroregenerative motif (NAV) derived from Activity Dependent Neuroprotective Protein (ADNP) with a self-assembling motif (K2[SL]6K2) to enhance neurite outgrowth. Interestingly, it is observed that this engineered hydrogel displays a network of prominent, directed filament-like structures. Remarkably, it supports and promotes the directional differentiation and alignment of stem cells into neuronal lineages. Motivated by this result, its therapeutic potential is evaluated in a rat model of sciatic nerve injury, where guided nerve regeneration is essential. The hydrogel is applied as a nerve guidance matrix, leading to structural repair and significant functional recovery of the injured nerve within two weeks. These findings are further supported by behavioral assessments, histological analysis, and evaluation of gastrocnemius muscle reinnervation. Overall, the results demonstrate that this engineered peptide hydrogel effectively promotes nerve regeneration and functional restoration, highlighting its promise as a therapeutic platform for peripheral nerve injury.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e03429"},"PeriodicalIF":9.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0