Biomaterials最新文献

A poly(lipoic acid)-based elastomer adhesive with synergistic activity of microenvironment regulation and peripheral neuropathy repair facilitates infectious diabetic wound healing 具有微环境调节和周围神经病变修复协同作用的聚硫辛酸弹性体胶粘剂促进感染性糖尿病创面愈合

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-10 DOI: 10.1016/j.biomaterials.2025.123489

Haotian Song , Feng Fu , Yueyang Chen , Rong Yang , Zhen Luo , Jiaxing Shao , Ying Qi , Qian Zhang , Jingjing Wang , Hongtao Sun , Chunyan Cui , Wenguang Liu

{"title":"A poly(lipoic acid)-based elastomer adhesive with synergistic activity of microenvironment regulation and peripheral neuropathy repair facilitates infectious diabetic wound healing","authors":"Haotian Song , Feng Fu , Yueyang Chen , Rong Yang , Zhen Luo , Jiaxing Shao , Ying Qi , Qian Zhang , Jingjing Wang , Hongtao Sun , Chunyan Cui , Wenguang Liu","doi":"10.1016/j.biomaterials.2025.123489","DOIUrl":"10.1016/j.biomaterials.2025.123489","url":null,"abstract":"<div><div>Diabetic wounds are notorious for their difficulty in healing and high recurrence rate, due to their harsh inflammatory microenvironment and the peripheral neuropathy caused by a hyperglycemic condition. However, current research often overlooks the impact of diabetic peripheral neuropathy on hindering wound healing. Here, we develop an adhesive elastomeric wound dressing by combining the natural active molecule lipoic acid (LA) with the clinically applied hypoglycemic drug metformin (Met), integrating the characteristics of remodeling the wound microenvironment and restoring peripheral nerve function, while stabilizing polyLA through the formation of strong salt bridge hydrogen bonds between guanidyl and carboxyl. The surface of the elastomeric dressing rich in adhesive carboxyl groups can achieve effective sealing protection for diabetic wounds by forming multiple hydrogen bonds or electrostatic interactions with the tissues, thereby resisting harmful bacterial invasion. The sustained targeted release of LA and Met at the wound site can effectively reduce local oxidative stress, increase collagen deposition and angiogenesis, and significantly upregulate neuroendocrine chemicals and nerve fiber markers, promoting the recovery of peripheral nerves around the lesion. In the diabetic infection model in rats, the <strong>polyLA-Met</strong> patch shows a significantly superior wound healing effect compared to the commercial 3M dressing.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123489"},"PeriodicalIF":12.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro-/nanomotors as platforms for gas therapy 微/纳米马达作为气体治疗的平台

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-09 DOI: 10.1016/j.biomaterials.2025.123492

Shuangjiao Sun, Ya Liu, Shuhuai Wang, Qinyi Gui, Wei Liu, Wei Long

{"title":"Micro-/nanomotors as platforms for gas therapy","authors":"Shuangjiao Sun, Ya Liu, Shuhuai Wang, Qinyi Gui, Wei Liu, Wei Long","doi":"10.1016/j.biomaterials.2025.123492","DOIUrl":"10.1016/j.biomaterials.2025.123492","url":null,"abstract":"<div><div>Active delivery of therapeutic gases for disease intervention is an appealing but challenging task that requires breakthroughs in nanomaterial-based delivery systems. Micro-/nanomotors (MNMs) capable of efficiently converting diverse forms of energy into mechanical motion have inspired innovations in the gas delivery and therapy domains, offering an alternative possibility to address the challenges of targeted delivery and controlled gas release during therapy. This review thus comprehensively summarizes recent advances in employing MNMs as mobile platforms for precise gas delivery and therapy. The review begins with an introduction of the physiological functions of diverse therapeutic gases, including NO, H<sub>2</sub>S, CO, O<sub>2</sub>, and H<sub>2</sub>. Then various proof-of-concept designs of artificial MNMs that can efficient propulsion in complex biological environments and intelligently release these gases in response to intrinsic or extrinsic stimuli are discussed. Particular emphasis has been placed on their potential in microenvironment modulation for disease treatment, aiming to demonstrate the distinct superiority of MNMs in this area. In addition, the key challenges and limitations of current MNMs utilized for gas therapy are addressed. It is believed that in the near future, MNMs will become sophisticated delivery platforms for facilitating gas therapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123492"},"PeriodicalIF":12.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A soft-hard hybrid scaffold for osteochondral regeneration through integration of composite hydrogel and biodegradable magnesium 复合水凝胶与可生物降解镁结合制备软硬复合骨软骨再生支架

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-09 DOI: 10.1016/j.biomaterials.2025.123493

Zijie Pei , Haojing Xu , Minzheng Guo , Weiyun Xu , Ya Wen , Fengpo Sun , Tongyi Zhang , Bo Peng , Piqian Zhao , Liangkun Huang , Mengyu Wang , Zhaoshuo He , Junzhi Liu , Zhichao Yang , Ze Zhang , Peng Wen , Liangyuan Wen

{"title":"A soft-hard hybrid scaffold for osteochondral regeneration through integration of composite hydrogel and biodegradable magnesium","authors":"Zijie Pei , Haojing Xu , Minzheng Guo , Weiyun Xu , Ya Wen , Fengpo Sun , Tongyi Zhang , Bo Peng , Piqian Zhao , Liangkun Huang , Mengyu Wang , Zhaoshuo He , Junzhi Liu , Zhichao Yang , Ze Zhang , Peng Wen , Liangyuan Wen","doi":"10.1016/j.biomaterials.2025.123493","DOIUrl":"10.1016/j.biomaterials.2025.123493","url":null,"abstract":"<div><div>Osteochondral injuries are prevalent and difficult to treat in clinical practice. Traditional tissue engineering typically results in poor integration at the calcified cartilage interlayer, since they cannot address different needs from the cartilage and the supporting subchondral bone. This study presents a hybrid biological scaffold integrating soft and hard components to systematically adopt to osteochondral regeneration. The upper section consists of bioactive hydrogel, kartogenin (KGN), and bone marrow stromal cells (BMSCs), replicating mechanical properties and chondrogenic potential of nature hyaline cartilage. The lower section utilizes a biodegradable metal magnesium (Mg) alloy scaffold with customized porous structure, providing mechanical response comparable to trabecular bone, along with regulated degradation and enhanced angiogenesis and osteogenesis. The bioactive hydrogel is compressed into the pores of Mg scaffold. Notably, the unique combination not only significantly improves mechanical response and fatigue resistance of the cartilage section but also maintains interface stability throughout the repair process. Accordingly, the hybrid scaffold effectively promotes the regeneration of both cartilage and subchondral bone simultaneously by upregulation of osteogenic and chondrogenic specific genes. Overall, this work provides valuable insights for treating osteochondral injuries by material–structure–function integrated strategies.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123493"},"PeriodicalIF":12.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron acceptor motif-manipulated NIR-II AIE photosensitizers synergically induce tumor pyroptosis through multimodal image-guided pure type I photodynamic and photothermal therapy 电子受体基元操纵的NIR-II AIE光敏剂通过多模态图像引导的纯I型光动力和光热疗法协同诱导肿瘤热亡

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-09 DOI: 10.1016/j.biomaterials.2025.123490

Chunbai Xiang , Yu Liu , Qihang Ding , Ting Jiang , Chao Li , Jingjing Xiang , Xing Yang , Yue Wang , Ting Yang , Wenxue Tong , Kun Qian , Qi Zhao , Zhiyun Lu , Zhen Cheng , Ping Gong

{"title":"Electron acceptor motif-manipulated NIR-II AIE photosensitizers synergically induce tumor pyroptosis through multimodal image-guided pure type I photodynamic and photothermal therapy","authors":"Chunbai Xiang , Yu Liu , Qihang Ding , Ting Jiang , Chao Li , Jingjing Xiang , Xing Yang , Yue Wang , Ting Yang , Wenxue Tong , Kun Qian , Qi Zhao , Zhiyun Lu , Zhen Cheng , Ping Gong","doi":"10.1016/j.biomaterials.2025.123490","DOIUrl":"10.1016/j.biomaterials.2025.123490","url":null,"abstract":"<div><div>Pyroptosis, a form of programmed cell death, is known for its strong capacity to induce immunogenic cell death (ICD), triggering the release of damage-associated molecular patterns (DAMPs) that amplify cancer immunotherapy. Recently, photocontrolled pyroptosis has emerged as a promising strategy within photodynamic therapy (PDT). Nonetheless, most existing photosensitizers exhibit a reliance on both type I and type II reactive oxygen species (ROS) generation, which not only leads to suboptimal efficacy in hypoxic tumor environments but also limits therapeutic depth and selectivity. In this study, we report a lysosome-targeted aggregation-induced emission (AIE) photosensitizer, <strong>PTQ-TPA3</strong>, engineered through receptor unit loop fusion and rotor integration via molecular evolution strategies. <strong>PTQ-TPA3</strong> uniquely achieves highly efficient pure type I ROS generation, alongside near-infrared-II (NIR-II) fluorescence emission and photothermal conversion properties. Under the guidance of multimodal imaging modalities, including photoacoustic, NIR-II fluorescence, and photothermal imaging, <strong>PTQ-TPA3</strong> nanoparticles enable synergistic photodynamic and photothermal therapy to drive robust ICD-mediated phototherapy. Furthermore, <strong>PTQ-TPA3</strong> demonstrates exceptional efficacy in hypoxic tumor environments by producing pure type I ROS while leveraging its photothermal effect to induce pyroptosis. This dual mechanism effectively eradicates cancer cells and stimulates systemic antitumor immunity, paving the way for innovative therapeutic strategies.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123490"},"PeriodicalIF":12.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft tissue integration around dental implants: A pressing priority 植牙周围的软组织整合：当务之急

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-09 DOI: 10.1016/j.biomaterials.2025.123491

Revathi Alexander , Xiaohua Liu

{"title":"Soft tissue integration around dental implants: A pressing priority","authors":"Revathi Alexander , Xiaohua Liu","doi":"10.1016/j.biomaterials.2025.123491","DOIUrl":"10.1016/j.biomaterials.2025.123491","url":null,"abstract":"<div><div>While osseointegration has traditionally been the focal point of dental implant design, recent research highlights the equally crucial role of establishing a resilient and biologically integrated soft tissue seal for long-term implant success. This review critically examines recent advances (primarily from the past five years) that elucidate the molecular, cellular, and materials science strategies essential for enhancing peri-implant soft tissue integration. Key factors include precisely engineered surface topographies at micro- and nanoscale levels, surface chemical modifications that enhance wettability and protein adsorption, and biomimetic coatings incorporating extracellular matrix-derived peptides, chemokines, and growth factors. Recent studies underscore the impact of laser micro- and nano-texturing, plasma treatments, and biofunctionalization in modulating fibroblast and epithelial cell behaviors, accelerating tissue attachment, and mitigating early inflammatory responses. Emerging implant-abutment designs, such as platform switching and transmucosal zirconia abutments, demonstrate improved soft tissue stability and reduce crestal bone loss. Additionally, the immunomodulatory potential of next-generation materials offers promising avenues for directing macrophage polarization and enhancing wound resolution. Collectively, this review synthesizes the latest evidence on material-driven and biological strategies for engineering a stable soft tissue interface. It provides a translational roadmap for the development of implant systems optimized for long-term soft tissue health, addressing a critical unmet need in dental implantology.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123491"},"PeriodicalIF":12.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microenvironment-activatable nanoagent for real-time NIR-II monitoring and targeted therapy of arterial restenosis 微环境可激活纳米剂用于动脉再狭窄的实时NIR-II监测和靶向治疗

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-07 DOI: 10.1016/j.biomaterials.2025.123482

Xianshe Meng , Jianwen Song , Huawen Meng , Chenzhong Liao , Zequn Yin , Ke Gong , Deling Kong , Ji Qi , Yajun Duan

{"title":"Microenvironment-activatable nanoagent for real-time NIR-II monitoring and targeted therapy of arterial restenosis","authors":"Xianshe Meng , Jianwen Song , Huawen Meng , Chenzhong Liao , Zequn Yin , Ke Gong , Deling Kong , Ji Qi , Yajun Duan","doi":"10.1016/j.biomaterials.2025.123482","DOIUrl":"10.1016/j.biomaterials.2025.123482","url":null,"abstract":"<div><div>Arterial restenosis is a critical risk factor for life-threatening cardiovascular diseases. Precise intervention and real-time monitoring are extremely important but remain major clinical challenges. Here, we present an advanced theranostic nanoagent that integrates hypoxia-responsive second near-infrared (NIR-II) fluorescence imaging with hypoxia-activatable anti-proliferative therapy for real-time diagnostics and precision treatment. This nanoplatform is constructed by co-encapsulating a novel <em>N</em>-oxide-based molecular probe and a hypoxia-activatable prodrug tirapazamine (TPZ) into osteopontin (OPN)-targeted liposomes. Under hypoxic conditions, the <em>N</em>-oxide probe undergoes conversion to its amine derivative, altering the intramolecular charge transfer properties and triggering turn-on NIR-II fluorescence signal. This property enables high-sensitivity, real-time monitoring of restenosis lesions in vivo. The nanoplatform exhibits dual hypoxia-responsive functionality: TPZ is selectively activated in hypoxic vascular lesions to inhibit vascular smooth muscle cell proliferation, and sustained OPN-mediated targeting promotes vascular repair. In guidewire-induced restenosis models, this system achieves simultaneous real-time monitoring of lesion progression via NIR-II imaging and significantly reduce restenosis while enhancing re-endothelialization. This study offers a promising strategy for developing high-performance theranostic nanoplatforms, enabling precise detection and improved treatment of restenosis-related diseases.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123482"},"PeriodicalIF":12.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasal-to-brain delivery of CCR5 antagonist for reshaping the dysregulated microglia-neuron axis and enhancing post-traumatic cognitive function CCR5拮抗剂经鼻至脑可重塑失调的小胶质神经元轴并增强创伤后认知功能

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-07 DOI: 10.1016/j.biomaterials.2025.123479

Pengcheng Zhang , Yaxin Wang , Yigang Xu , Xueping Li , Xuya Yu , Yi Li , Dunwan Zhu , Fang Luo , Wen Li , Xu Jin

{"title":"Nasal-to-brain delivery of CCR5 antagonist for reshaping the dysregulated microglia-neuron axis and enhancing post-traumatic cognitive function","authors":"Pengcheng Zhang , Yaxin Wang , Yigang Xu , Xueping Li , Xuya Yu , Yi Li , Dunwan Zhu , Fang Luo , Wen Li , Xu Jin","doi":"10.1016/j.biomaterials.2025.123479","DOIUrl":"10.1016/j.biomaterials.2025.123479","url":null,"abstract":"<div><div>The dysregulation of the microglia-neuron axis plays a pivotal role in the pathogenesis of cognitive dysfunction following traumatic brain injury (TBI). The C–C chemokine receptor 5 (CCR5), markedly upregulated on both microglia and neurons post-injury, serves as a crucial mediator in the neuroinflammatory response and consequent neurological deficits. However, the therapeutic application of CCR5 antagonists in TBI is impeded by the delivery barriers presented by the blood-brain barrier (BBB) and their limited neuron-targeting efficacy. In this study, we introduce a novel nasal-to-brain delivery nanoplatform designed to facilitate the efficient brain delivery of DAPTA, a peptide antagonist of CCR5, aiming to inhibit CCR5 signaling and improving cognitive function following TBI. Biocompatible chitosan nanocarriers grafted with cell-penetrating peptide (TAT) and neuron-binding lactoferrin (Lf) were initially fabricated, demonstrating substantial DAPTA loading capacity, active mucosal and neural transportation, and enhanced neuron-targeting capabilities. The dual-engineered nanodrugs (DA@LT NPs) effectively penetrated the trigeminal and olfactory nerves, significantly enhancing the transport of DAPTA into the brain following intranasal delivery. In a TBI-induced mouse model, DA@LT NPs markedly alleviated the neuroinflammatory response, promoted M2 microglia polarization, protected neurons from pyroptosis, and improved both motor and cognitive functions of animals. The non-invasive intranasal delivery of the therapeutic CCR5 peptide antagonist using these mucus-penetrating and neuron-targeting nanoformulations presents a promising intervention for ameliorating neurological inflammation and cognitive impairments associated with TBI.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123479"},"PeriodicalIF":12.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIR-activated nanodisguisers for targeted bactericidal action and enhanced electron transfer in periodontitis treatment nir激活的纳米伪装剂在牙周炎治疗中的靶向杀菌作用和增强电子转移

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-07 DOI: 10.1016/j.biomaterials.2025.123487

Manlin Qi , Qihang Ding , Yujia Shi , Kun Wang , Jia Liu , Jing Zhou , Wei Zhang , Chengyu Liu , Shuang Liang , Biao Dong , Jong Seung Kim , Lin Wang

{"title":"NIR-activated nanodisguisers for targeted bactericidal action and enhanced electron transfer in periodontitis treatment","authors":"Manlin Qi , Qihang Ding , Yujia Shi , Kun Wang , Jia Liu , Jing Zhou , Wei Zhang , Chengyu Liu , Shuang Liang , Biao Dong , Jong Seung Kim , Lin Wang","doi":"10.1016/j.biomaterials.2025.123487","DOIUrl":"10.1016/j.biomaterials.2025.123487","url":null,"abstract":"<div><div>Antibacterial treatment for periodontitis faces significant challenges due to the lack of selective bactericidal therapies. In this study, we developed multifunctional nanospheres encapsulated with <em>Fusobacterium nucleatum</em>-derived outer membrane vesicles (OMVs) to target periodontal pathogens specifically. These OMVs act as a \"camouflage,\" allowing the nanospheres to infiltrate bacterial environments undetected, adhere to pathogen surfaces, and maximize therapeutic effects. Direct contact between nanospheres and bacteria accelerates electron transfer, and nanospheres trigger a proliferation of endogenous reactive oxygen species (ROS), leading to oxidative stress and bacterial death. Transcriptomic analysis confirmed that the nanospheres accelerated electron transfer activity and disrupted deoxyribonucleic acid (DNA) repair mechanisms and thiamine metabolism while enhancing bacterial respiration. Though supported by dual photodynamic and photothermal therapies under near-infrared light, the primary mechanism of action focuses on electron transfer and metabolic disruption. In vitro and <em>in vivo</em> experiments demonstrated the nanospheres’ potent biofilm eradication and periodontitis treatment efficacy, offering a promising new approach for selective bacterial targeting. This strategy targets pathogens effectively and preserves the beneficial microbiota, providing an innovative solution for treating periodontitis and other biofilm-related infections.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123487"},"PeriodicalIF":12.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrode stimulation parameter modifications elicit differential glial cell responses in vitro over a short 4-h timecourse 电极刺激参数的改变在体外短4小时的时间内引起胶质细胞的差异反应

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-06 DOI: 10.1016/j.biomaterials.2025.123478

Christopher T. Tsui , Soroush Mirkiani , David A. Roszko , Matthew J. Birtle , Anna E. DeCorby , Matthew A. Churchward , Vivian K. Mushahwar , Kathryn G. Todd

{"title":"Electrode stimulation parameter modifications elicit differential glial cell responses in vitro over a short 4-h timecourse","authors":"Christopher T. Tsui , Soroush Mirkiani , David A. Roszko , Matthew J. Birtle , Anna E. DeCorby , Matthew A. Churchward , Vivian K. Mushahwar , Kathryn G. Todd","doi":"10.1016/j.biomaterials.2025.123478","DOIUrl":"10.1016/j.biomaterials.2025.123478","url":null,"abstract":"<div><div>A cell culture model to assess glial cell responses to electrically stimulating electrodes in real-time was developed. Our previous work measured glial cell responses to stimulation paradigms and highlighted the importance of electrical stimulation considerations when designing a biocompatible neural interfacing device. The formation of voids around stimulating platinum-iridium electrodes also prompted an investigation into the fate of cells that would have once populated that area. Live-imaging experiments involving EGFP-positive microglia from heterozygous CX3CR-1<sup>+/EGFP</sup> mice were designed. Live-imaging animations over 4 h showed necrotic microglial cell death around stimulating electrodes. The degree to which this was occurring was further analyzed by electrically stimulating mixed glia and modifying parameters such as stimulation amplitude (0.1–0.4 mA), waveform shape (rectangular/sinusoidal/ramped), and frequency (25–55 Hz). The different stimulation parameters had differential effects on glial cell biomarker signal outputs (cell density, fluorescence intensity, area coverage). Scanning electron microscopy and energy-dispersive x-ray spectroscopy of the electrode surfaces post-stimulation did not reveal any significant damage or changes to surface elemental composition. Finally, electrochemical testing of the proposed <em>in vitro</em> setup revealed influences of different components of the mixed glial cell cultures towards the electrochemical performance of the electrodes in terms of cathodic charge storage capacity, impedance, phase angle, and voltage transient excursions. The results highlight the impact that electrical stimulation parameters have on glial cell fate at the electrode-cell culture interface, and provide data towards refinement of stimulation paradigms used in electrical neuromodulation applications.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123478"},"PeriodicalIF":12.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiol-ene photoclick hydrogels reinforced with poly(protocatechualdehyde)-coated gallium doped bioactive glass nanoparticles for scarless healing of infected wound 聚（原儿茶醛）包被掺杂镓的生物活性玻璃纳米颗粒增强的硫醇烯光点击水凝胶用于感染伤口的无疤痕愈合

IF 12.8 1区医学

Biomaterials Pub Date : 2025-06-06 DOI: 10.1016/j.biomaterials.2025.123469

Xinyi Jian , Dun Liu , Xuexue Liu , Xingping Zhao , Yongjie Zhu , Sanqiang Xia , Enuo Peng , Mengran Zhao , Jie Yi , Guohua Jiang , Dabao Xu , Kui Cheng , Wenjian Weng , Zezhang Zhu , Benlong Shi , Bolin Tang

{"title":"Thiol-ene photoclick hydrogels reinforced with poly(protocatechualdehyde)-coated gallium doped bioactive glass nanoparticles for scarless healing of infected wound","authors":"Xinyi Jian , Dun Liu , Xuexue Liu , Xingping Zhao , Yongjie Zhu , Sanqiang Xia , Enuo Peng , Mengran Zhao , Jie Yi , Guohua Jiang , Dabao Xu , Kui Cheng , Wenjian Weng , Zezhang Zhu , Benlong Shi , Bolin Tang","doi":"10.1016/j.biomaterials.2025.123469","DOIUrl":"10.1016/j.biomaterials.2025.123469","url":null,"abstract":"<div><div>Complex pathological microenvironment mainly characterized by excessive inflammation, reactive oxygen species (ROS) production, drug-resistant bacterial infection makes wound rapid scarless healing still a major clinical challenge. Inspired by skin composed principally of collagen and mucopolysaccharides, an injectable nanocomposite hydrogel with immunoregulation, antioxidant and antibacterial activity based on allyl glycidyl ether grafted gelatin (Gel-AGE), cysteamine grafted hyaluronic acid (HA-CSA) and poly(protocatechualdehyde)-coated gallium doped bioactive glass (PPA@GaBG) nanoparticles, was designed for treatment of infected wound. The PPA@GaBG/Gel-AGE/HA-CSA (PGBGH) hydrogel can be rapidly formed via thiol-ene photo-click chemistry between Gel-AGE and HA-CSA. In PGBGH hydrogel, the presence of core-shell PPA@GaBG significantly enhanced the antioxidant ability of hydrogels and M2 polarization of macrophages, as well as improving mechanical and tissue adhesive properties of the hydrogels via the formation of Schiff base bonds between PPA and gelatin/skin tissue. Moreover, the PPA shell effectively inhibited the ion burst release of Ga<sup>3+</sup> observed in GaBG/GH hydrogels, which improved the biocompatibility of the PGBGH hydrogel while maintaining excellent antibacterial activity. As a result of multiple functions of the PPA@GaBG, the PGBGH hydrogel promoted angiogenesis, granulation tissue formation and re-epithelialization with improved deposition ratio of collagen III/I, which achieved the rapid scarless healing of infected wound.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"324 ","pages":"Article 123469"},"PeriodicalIF":12.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0