Biomaterials最新文献

{"title":"Reversible shear stress-mediated mechanoregulation of endothelial cell function in thixotropic hydrogels via L-type Ca2+ channel and focal adhesion molecules for accelerated vascularization","authors":"Zhongtao Li ,&nbsp;Sumei Li ,&nbsp;Rongwei Cui ,&nbsp;Tao Jing ,&nbsp;Haodong Peng ,&nbsp;Ran Wang ,&nbsp;Jie Weng ,&nbsp;Chaoming Xie ,&nbsp;Feng Lin ,&nbsp;Xue Gou ,&nbsp;Shuxin Qu","doi":"10.1016/j.biomaterials.2025.123556","DOIUrl":"10.1016/j.biomaterials.2025.123556","url":null,"abstract":"<div><div>Developing functional vascular networks in engineered tissues is crucial for regenerative medicine. Recently, thixotropic hydrogel has emerged as a promising approach due to their 3D-printability and force-responsive dynamics. However, their gel-sol transitions under physiological loading and subsequent mechanoregulation mechanism on vascularization remains inadequately explored. Here, the reversible shear stress induced in thixotropic hydrogels under bionic cyclic stretching (5 % strain, and 0.5, 1 or 1.5 Hz) has been demonstrated to significantly accelerate endothelial cell adhesion, migration, and angiogenesis. These dynamic mechanical responses are precisely quantified and monitored through computational simulations and specially designed experimental apparatus. Mechanistic investigations reveals that the mechanically regulated cell behavior is mediated by cell adhesion molecules and calcium signaling pathways, which can be inhibited using Talin bloker (e.g., neomycin) and L-type voltage-gated calcium channel antagonists (e.g., verapamil), respectively. Furthermore, subcutaneous implantation of thixotropic hydrogels in rats results in denser and more rapid vascularization compared to non-thixotropic hydrogels. The reversible shear stress-regulated vascularization strategy is anticipated to offer a novel and efficient approach for constructing functional blood vessels in regenerative medicine.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123556"},"PeriodicalIF":12.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MXenzyme-reinforced hydrogel enhances stem cell therapy through multidimensional regulation in rheumatoid arthritis management","authors":"Yue Zhao ,&nbsp;Zuhao Li ,&nbsp;Xiao Chen ,&nbsp;Yan Hu ,&nbsp;Yuanwei Zhang ,&nbsp;Jiacan Su ,&nbsp;Yanli Zhao","doi":"10.1016/j.biomaterials.2025.123560","DOIUrl":"10.1016/j.biomaterials.2025.123560","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a devastating autoimmune disorder that imposes health and economic burdens on communities worldwide. Although stem cell transplantation has emerged as a promising option for RA management, the osteoimmune microenvironment that characterizes elevated reactive oxygen/nitrogen species (ROS/RNS) and poor oxygen (O₂) supply compromise therapeutic efficiency. To address this issue, we present a nanozyme-reinforced hydrogel designed to modulate the immune microenvironment using a 'turning foes into friends' strategy, thereby enhancing the outcomes of stem cell therapy in RA. This hydrogel scavenges excessive ROS/RNS while synergistically generating O₂, making it an effective protective vehicle for bone marrow-derived mesenchymal stem cells (BMSCs). Moreover, it successfully induces macrophage polarization from the M1 to the M2 phenotype and facilitates the osteogenic differentiation of BMSCs, even under hostile oxidative conditions. Furthermore, hydrogel-mediated stem cell therapy is demonstrated to attenuate the onset of exacerbated synovial inflammation and promote bone remodeling in a severe rabbit RA model. This study offers a promising avenue for augmented stem cell therapy efficiency in RA management and provides translational significance for stem cell-based therapy even beyond autoimmune disorders.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123560"},"PeriodicalIF":12.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable hollow MnO2 decorated by carbon dots with cholesterol depletion capability for cascaded amplification of sono-immunotherapy","authors":"Jingru Zhao ,&nbsp;Jinming Cai ,&nbsp;Jinyan Hu ,&nbsp;Zhenlin Zhang ,&nbsp;Yuan-Yuan Liu ,&nbsp;Dengyu Pan ,&nbsp;Longxiang Shen ,&nbsp;Bijiang Geng","doi":"10.1016/j.biomaterials.2025.123559","DOIUrl":"10.1016/j.biomaterials.2025.123559","url":null,"abstract":"<div><div>For the effective activation of the adaptive immune response, it is crucial to promote the maturation of dendritic cells (DCs) and subsequently initiate cytotoxic T lymphocytes. Nevertheless, the immunosuppressive tumor microenvironments (TME) are believed to hinder DC maturation, leading to a significant decrease in the effectiveness of immunotherapy. In this work, we present the first instance of combining ROS-triggered immunogenic cell death (ICD), cholesterol depletion, and STING activation to achieve the cascade amplification of antitumor immune response. The Zr-based metal-organic frameworks (MOF) is utilized as the template for the synthesis of hollow MnO₂ (H–MnO₂). Carbon dots (CDs) with cholesterol depletion capability are then deposited on H–MnO₂ to form heterojunctions. CD@H–MnO₂ not only has improved ROS generation ability under ultrasound due to heterojunction construction, but also shows GSH-responsive degradation properties, enabling the targeted release of CDs and Mn ions in tumors. CD@H–MnO₂-triggered cascade amplification of antitumor immune response is elucidated as follows: (1) Heterojunction construction, GSH depletion, and relief of hypoxia co-augmented ROS yield could significantly induce a robust ICD effect. (2) The released Mn ions stimulate DC maturation by activating the cGAS-STING pathway. (3) Direct enhancement of T cell toxicity can be realized by CDs through depleting cholesterol. Notable antitumor effects have been observed to eliminate primary tumors and stop the growth of distant tumors. This study presents a novel method to merge ROS-triggered ICD, cholesterol depletion, and STING activation into one nanomaterial to produce long-lasting and powerful immune responses.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123559"},"PeriodicalIF":12.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoinitiated CVD antifouling coatings enable long-term stability of flexible multifunctional neural probes for chronic neural recording","authors":"Yunyoung Choi ,&nbsp;Woojin Jeon ,&nbsp;Yeji Kim ,&nbsp;Hakchun Kim ,&nbsp;Younghak Cho ,&nbsp;Yerim Jang ,&nbsp;Somin Lee ,&nbsp;Daehun Kim ,&nbsp;Tae Jin Mun ,&nbsp;Youngmin Yoo ,&nbsp;Inhee Choi ,&nbsp;Sung Gap Im ,&nbsp;Seongjun Park ,&nbsp;Hyejeong Seong","doi":"10.1016/j.biomaterials.2025.123554","DOIUrl":"10.1016/j.biomaterials.2025.123554","url":null,"abstract":"<div><div>Flexible neural probes with integrated recording, optical stimulation, and drug delivery capabilities offer unprecedented access to neural circuit dynamics. However, their long-term utility is compromised by foreign body responses that isolate recording sites from target neurons. This study introduces photoinitiated chemical vapor deposition (piCVD) as a transformative approach to neural interface stability through ultrathin (&lt;100 nm) anti-fouling coatings. Unlike conventional hydrogel coatings that impair electrical signal transmission, our piCVD-applied poly(2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) coating maintains electrical functionality by preserving low impedance while providing superior anti-fouling properties. <em>In vitro</em> protein adsorption studies demonstrated near-complete resistance to both albumin and fibrinogen compared to uncoated surfaces, with the coating maintaining stability even after 24 h of sonication—durability unachievable with conventional wet-chemistry methods. When evaluated in mouse models over three months, the coated probe maintained high-quality spontaneous neural recordings and optically evoked potentials throughout the study period, with signal-to-noise ratios improving from 18.0 at week 1–20.7 at week 13. This performance significantly correlates with 66.6 % reduction in glial scarring, 84.6 % increase in neuronal preservation compared to uncoated probes. The specific combination of CVD methodology and optimized copolymer composition achieves long-term stability, representing a significant advance over the typical one-month limitation of conventional coatings. These results establish piCVD antifouling coatings as an enabling technology for chronic neural interfaces in both basic neuroscience research and emerging neuroprosthetic applications.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123554"},"PeriodicalIF":12.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking gingival endotoxin tolerance to modulate immune balance for tumor postoperative wound management","authors":"Daping Xie ,&nbsp;Zhencheng Liao ,&nbsp;Chonghao Chen ,&nbsp;Yuwei Li ,&nbsp;Zijun Zheng ,&nbsp;Yiming Niu ,&nbsp;Dandan Xia ,&nbsp;Yufeng Zheng ,&nbsp;Chunming Wang","doi":"10.1016/j.biomaterials.2025.123558","DOIUrl":"10.1016/j.biomaterials.2025.123558","url":null,"abstract":"<div><div>Postoperative management after tumor resection poses a significant clinical challenge as it must satisfy two demands – tumor eradication and tissue repair, which require contradictory immunological characteristics. Current treatment strategies have limitations as they usually fulfil one of the two requirements. Here, inspired by the unique mechanism of bacteria-triggered endotoxin tolerance (ET) in maintaining tissue homeostasis in the periodontal tissue, we devise a bioactive scaffold to establish an ET-mimicking immune niche in the local tissue of tumor resection. This glucomannan derivative-based electrospun scaffold (GMES) efficiently stimulates macrophages towards an ET state <em>via</em> a toll-like receptor-2 (TLR2)-dependent manner that, on the one hand, overexpresses pro-inflammatory factors and macrophage receptor with collagenous structure (MARCO) to suppress tumor recurrence and, on the other hand, release anti-inflammatory and pro-regenerative factors to promote wound healing. Our <em>in vivo</em> tests in three models of tumor recurrence, post-resection wounds and tumor metastasis show that GMES promotes healing at the tumor resection site while preventing tumor recurrence or metastasis. Our strategy represents a novel, microbial signal-inspired approach to postoperative tumor management.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123558"},"PeriodicalIF":12.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically engineering cells to produce therapeutically boosted extracellular vesicles for cardiovascular calcification","authors":"Neil Patel ,&nbsp;Veronica LaMastro ,&nbsp;Joshua Giblin ,&nbsp;Elijah Avery ,&nbsp;Bari Noor ,&nbsp;Gregory Magee ,&nbsp;Eun Ji Chung","doi":"10.1016/j.biomaterials.2025.123552","DOIUrl":"10.1016/j.biomaterials.2025.123552","url":null,"abstract":"<div><div>Calcification associated with atherosclerosis is a major driver of morbidity globally. Despite the correlation of calcification with plaque rupture and sudden death, there are no clinically approved therapies that treat vascular calcification. Notably, vascular smooth muscle cells (VSMCs) represent a promising target to inhibit vascular calcification, as VSMCs are the primary source of calcification deposition in the vasculature. To that end, we report a novel approach using extracellular vesicles (EVs) to deliver anti-osteogenic miR-133 to osteochondrogenic VSMCs in atherosclerosis. Traditionally, loading miRs into EVs is marred by low loading efficiency, inefficient EV modification, miR degradation, or loss of EV structural integrity. To address these challenges, VSMCs were transduced to create cell lines expressing miR-133 modified with ExoMotifs, or 4–8 nucleotide motifs which enable binding to proteins involved in miR sorting, resulting in VSMCs that secrete EVs highly loaded with miR-133. Additionally, EVs were surface functionalized with a hydroxyapatite binding peptide (HABP) to enable targeting to areas of vascular calcification. Our results show that HABP-miR-133-EVs can inhibit osteochondrogenic VSMCs, promote contractile VSMCs genes, and inhibit vascular calcification both <em>in vitro</em> and <em>in vivo</em> in murine atherosclerosis models. More broadly, we demonstrate a platform strategy to develop cellular factories for miR-loaded, therapeutic EVs that can be tailored for a variety of diseases.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123552"},"PeriodicalIF":12.8,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational contrast agents for use in fluorescence image-guided tumor surgery","authors":"Da-Yong Hou ,&nbsp;Xiang-Peng Li ,&nbsp;Yue-Ze Wang ,&nbsp;Peng Zhang ,&nbsp;Jiong-Cheng Wu ,&nbsp;Hui-Hui You ,&nbsp;Mei-Yu Lv ,&nbsp;Shi-An Zhou ,&nbsp;Xiao Liu ,&nbsp;Gao Zhang ,&nbsp;Hong-Wei An ,&nbsp;Hao Wang ,&nbsp;Wanhai Xu","doi":"10.1016/j.biomaterials.2025.123549","DOIUrl":"10.1016/j.biomaterials.2025.123549","url":null,"abstract":"<div><div>Currently, surgical intervention remains the foundation in curing most solid tumors. Over the past few decades, even with rapid intraoperative tissue pathology assessment, the overall rate of positive surgical margins for tumors stagnated between 15 % and 60 %. The increased local recurrence rates and poor prognoses associated with various cancers, such as head and neck, brain, breast, lung, prostate, and gastrointestinal cancers, are linked to positive margins. Recently, driven by the concept of precise surgery, significant advancements have been reported in the intraoperative use of fluorescent contrast agents in the field of surgery. Molecular imaging via intraoperative fluorescence plays a guiding role in surgery, providing surgeons with visible fluorescent images. In clinical applications, fluorescent contrast agents can clearly delineate tumor boundaries, offering high recognition capacities and real-time guidance during surgery. Additionally, they can localize lymph node metastases, detect small metastatic lesions, and identify critical anatomical structures during surgery, thus reducing the risk of collateral damage. An ideal surgical guidance technology should lack radiation and display a high sensitivity and good resolution and an adjustable field of view, with rapid imaging. Enhancing the tissue penetration of fluorescence and targeting capacities of molecular probes are critical in providing more comprehensive tumor-related data. Additionally, screening and identifying other tumor biomarkers and more of their corresponding targeted molecular data are essential in enhancing the specificity of molecular imaging. Finally, the investigation and use of a wider range of near-infrared fluorescent dyes with improved biocompatibilities and imaging are critical in designing synthetic probes for application in intraoperative navigation. The development of novel, efficient, safe fluorescent contrast agents is a critical direction in current pharmaceutical research and development.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123549"},"PeriodicalIF":12.8,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contactless 3D acoustic assembly of liquid capsules for bottom-up tissue engineering","authors":"Maria Eduarda Rocha Luiz ,&nbsp;Mariana Carreira ,&nbsp;Sara Nadine ,&nbsp;Riccardo Tognato ,&nbsp;Romedi Parolini ,&nbsp;Syeda M. Bakht ,&nbsp;Tiziano Serra ,&nbsp;João F. Mano","doi":"10.1016/j.biomaterials.2025.123555","DOIUrl":"10.1016/j.biomaterials.2025.123555","url":null,"abstract":"<div><div>In recent years, considerable efforts have been directed towards developing systems that replicate native tissue microarchitecture, enhancing cell viability and achieving close-to-native cellular organization. Despite advancements in various assembly methods, scalability and cell viability remain challenging due to the time consuming nature of certain approaches. Acoustic assembly has emerged as a powerful technology for modular units' assembly, leveraging sound waves to achieve rapid, contactless spatial arrangement by fine-tuning parameters such as frequency, amplitude, and chamber geometry. Here we present a system that employs acoustic waves to generate spatial patterns of liquid-core microcapsules, encapsulating poly-caprolactone surface-functionalized microparticles and umbilical cord-derived mesenchymal stem cells. The microcapsules were produced using electrohydrodynamic atomization in conjugation with an aqueous two-phase system and subsequently embedded in gelatin methacrylate. Acoustic waves were then applied to assemble the liquid-core microcapsules in well-defined patterns within the hydrogel precursor followed by crosslinking for structural stability. This approach allows us to define spatial patterns with precision, aligning with simulation predictions. The liquid nature of the microcapsules’ core permits the organization of cells within the space towards the formation of microtissues decoupled from the external environment. The patterned constructs maintained cell viability for 14 days, facilitating the formation of microaggregates within liquid-core microcapsules and maintained organized microstructures. To explore the versatility of this system, we successfully patterned and stacked multiple layers of microcapsules, increasing structural complexity. Furthermore, we demonstrated its ability to support co-culture by seeding human umbilical vein endothelial cells onto the constructs as a proof of concept for promoting enhanced cellular interactions. This platform offers a scalable, versatile solution for developing tissue-mimetic multiscale constructs with tunable complexity, enabling rapid and non-contact assembly, making it a valuable tool for advancing in vitro models and studying complex cellular interactions.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123555"},"PeriodicalIF":12.8,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetically controlled microgelbots with stem cells for the treatment of interstitial cystitis","authors":"Hyunsik Choi ,&nbsp;Bolam Kim ,&nbsp;Yewon Seo ,&nbsp;Tae Yeon Kim ,&nbsp;Ki Wan Bong ,&nbsp;Sei Kwang Hahn","doi":"10.1016/j.biomaterials.2025.123551","DOIUrl":"10.1016/j.biomaterials.2025.123551","url":null,"abstract":"<div><div>Stem cell therapy has been widely investigated for the treatment of chronic bladder diseases such as interstitial cystitis/bladder pain syndrome (IC/BPS). However, the delivery of stem cells into the bladder wall is limited due to the mucus layer lining the bladder wall and the frequent urination, leading to the fast clearance of stem cells from the bladder. Here, we report a soft microgelbot (μgelbot) composed of a magnetic nanochain embedded microgel in a tunable size and shape for the enhanced delivery of mesenchymal stem cells (MSCs) into the bladder wall through the mucus layer. <em>In vitro</em> penetration tests to optimize the shape of μgelbots show that the quadrangle shaped μgelbots effectively apply a shear force to the surrounding shear-thinning mucus layer for the enhanced penetration under a rotating magnetic field. After loading MSCs onto the μgelbot, we confirm the enhanced penetration and retention in the reconstructed mucus layer. Finally, we successfully demonstrate the paracrine effects of MSCs loaded μgelbots on chronic IC murine models, inhibiting the mast cell infiltration, collagen deposition, and bladder cell apoptosis. Taken together, we could confirm the feasibility of magnetically controlled μgelbots as a promising platform for the stem cell therapy of IC/BPS.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123551"},"PeriodicalIF":12.8,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-facilitated peroxidase-like hydrogel with simultaneous wound infection monitoring and eliminating","authors":"Zhibin Li ,&nbsp;Guangtao Huang ,&nbsp;Xiangqiang Dai ,&nbsp;Mulan Qahar ,&nbsp;Zi Ye ,&nbsp;Minghui Wang ,&nbsp;Jiawen Deng ,&nbsp;Xiaoqi Zhang ,&nbsp;Yan Zhou ,&nbsp;Yu He ,&nbsp;Shi Chen ,&nbsp;Jun Wu","doi":"10.1016/j.biomaterials.2025.123542","DOIUrl":"10.1016/j.biomaterials.2025.123542","url":null,"abstract":"<div><div>Clinically, despite tremendous efforts having been devoted to preventing the invasive bacterial infection in acute and chronic wounds, the abuse or excessive use of antibacterial agents still poses great hurdles for wound management, which significantly raises the risk of detrimental side-effects and complications in the wound disinfection. Herein, we develop a hydrogel dressing of MPAH to attain real-time infection monitoring and on-demand antibacterial therapy. In this scenario, we propose a creative strategy of applying laser-facilitated MPAH photodecomposition of H₂O₂ to trigger the burst release of reactive oxygen species (ROS) and thus eliminate bacteria. Taking advantage of the visible indication of pH variability and laser-mediated ROS burst release, MPAH permits real-time monitoring of wound infection status and simultaneously endows it with superior on-demand antibacterial activity at a low H₂O₂ dosage. Beyond that, MPAH also provides a favorable healing environment synergistically promotes wound closure and re-epithelialization. With these merits, MPAH presents a vast potential for wound disinfection and simultaneously addresses the histotoxicity issues of routinely used medical H₂O₂ (3 %, V/V). Therefore, our study not only pioneers the exploration of a promising alternative antibacterial strategy, but more excitingly, also opens a profound vista for potential biomedical applications of laser-facilitated H₂O₂ photodecomposition.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"325 ","pages":"Article 123542"},"PeriodicalIF":12.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}