{"title":"Vanadium single-atoms coordinated artificial peroxidases as biocatalyst-linked immunosorbent assay for highly-sensitive carcinoembryonic antigen immunoassay.","authors":"Minjia Yuan, Rui Yan, Zhenyang Zhao, Qinlong Wen, Xiaodong Xie, Mohsen Adeli, Shuang Li, Chong Cheng","doi":"10.1016/j.biomaterials.2024.123008","DOIUrl":"10.1016/j.biomaterials.2024.123008","url":null,"abstract":"<p><p>In medical and biomedical fields, enzyme-mimetic nanomaterials have garnered significant interest as efficacious signal enhancers for biocatalyst-linked immunosorbent assays (BLISA). Despite the burgeoning enthusiasm, engineering artificial biocatalysts that exhibit both exceptional catalytic proficiency and pronounced colorimetric signal output remains a formidable challenge. Inspired by the heme structures and biocatalytic activities of horseradish peroxidase, we introduce the synthesis of vanadium single-atoms (SAV) coordinated artificial peroxidases as BLISA for highly sensitive and selective carcinoembryonic antigen (CEA) immunoassay. Our synthesized SAV exhibits peroxidase (POD)-like activity that is both efficacious and highly specific, surpassing the performance of many other single-atom-structured materials. The SAV-linked immunoassay demonstrates an ultrasensitive response to the target antigen (CEA), with a linear detection range spanning 0.03-10 ng/mL and an impressively low detection limit of 0.335 ng/mL. This straightforward and robust immunoassay technique not only achieves superior signal amplification compared to traditional natural enzymes but also boasts high precision, commendable reproducibility, and remarkable specificity, aligning closely with conventional enzyme-linked immunosorbent assay for CEA detection in serum samples. This study offers a blueprint for designing artificial peroxidase-based colorimetric nanosystems, promoting the evolution of ultrasensitive BLISA applications for the early diagnosis and intervention of cancer.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123008"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-12-06DOI: 10.1016/j.biomaterials.2024.122997
Ngoc Man Phan, Thanh Loc Nguyen, Dong Kwang Min, Jaeyun Kim
{"title":"Mesoporous polydopamine nanoparticle-based tolerogenic vaccine induces antigen-specific immune tolerance to prevent and treat autoimmune multiple sclerosis.","authors":"Ngoc Man Phan, Thanh Loc Nguyen, Dong Kwang Min, Jaeyun Kim","doi":"10.1016/j.biomaterials.2024.122997","DOIUrl":"10.1016/j.biomaterials.2024.122997","url":null,"abstract":"<p><p>Multiple sclerosis (MS) is a chronic neurological disorder derived from autoreactive immune system attacking the protective myelin sheath that surrounds nerves in the central nervous system (CNS). Here, a tolerogenic nanovaccine for generating an antigen-specific immune tolerance for treating MS is proposed. It consisted of a mesoporous polydopamine (mPDA) nanoparticle, characterized by high reactive oxygen species (ROS)-scavenging property, loaded with MS-derived autoantigen. Intravenous vaccination of autoantigen-loaded mPDA could induce tolerogenic dendritic cells (DCs) with low expression of co-stimulatory molecules while presenting peptide epitopes. The tolerogenic DCs induced peripheral regulatory T-cells (Tregs), thereby reducing infiltration of autoreactive CD4<sup>+</sup> T-cells and inflammatory antigen-presenting cells (APCs) into the CNS. In MS-mimicking mouse model, the tolerogenic nanovaccine prevented MS development in the early therapeutic setup and exhibited an enhanced recovery from complete paralysis in the late therapeutic model. The current platform could be exploited to treat other autoimmune diseases where disease-dependent autoantigen peptides are delivered.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"122997"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-12-05DOI: 10.1016/j.biomaterials.2024.123000
Xirui Wu, Junjun Zhang, Zheng Deng, Xianglong Sun, Yifan Zhang, Cai Zhang, Jiadong Wang, Xinke Yu, Guangbao Yang
{"title":"Bacteria-based biohybrids for remodeling adenosine-mediated immunosuppression to boost radiotherapy-triggered antitumor immune response.","authors":"Xirui Wu, Junjun Zhang, Zheng Deng, Xianglong Sun, Yifan Zhang, Cai Zhang, Jiadong Wang, Xinke Yu, Guangbao Yang","doi":"10.1016/j.biomaterials.2024.123000","DOIUrl":"10.1016/j.biomaterials.2024.123000","url":null,"abstract":"<p><p>Radiotherapy (RT) can trigger immunogenic cell death (ICD) in tumor cells and release adenosine triphosphate (ATP) to activate antitumor immunity. However, the formation of immunosuppressive adenosine (ADO) mediated by ectonucleotidases including CD39 and CD73, can exacerbate the immunosuppressive effects. Herein, a radiosensitizer-based metal-organic framework (MOF) composed of bismuth (Bi) and ellagic acid (EA) was synthesized in situ on the surface of Escherichia coli Nissle 1917 (EcN) to serve as a carrier for the CD39 inhibitor sodium polyoxotungstate (POM-1). This therapeutic platform, acting as a radiosensitizer, significantly enhances cytotoxicity against tumor cells while effectively inducing ICD and releasing high concentrations of ATP. Subsequently, the released POM-1 increases the levels of pro-inflammatory extracellular ATP while preventing tumor immunosuppression caused by the accumulation of ADO. Additionally, as a natural immune adjuvant, EcN further promotes the maturation of dendritic cells (DCs) and the infiltration of cytotoxic T lymphocytes (CTLs). As a result, such treatment initiates the destruction of established tumor growth and induces strong abscopal effects, leading to a significant inhibition of tumor metastases. This strategy presents a bacterial-based biohybrid system that facilitates RT-induced ICD while simultaneously limiting the degradation of ATP into ADO, thereby achieving sustained anti-tumor immunity.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123000"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In-situ Sprayed platelet-derived small extracellular vesicles for the skin flap survival by reducing PANoptosis.","authors":"Zhe Liu, De-Heng Chen, Zi-Hao Lin, Zi-Yi Wang, Hao Peng, Ruo-Tao Liu, Zhi-Chao Hu, Yao-Hua He, Xiao-Juan Wei, Chang-Qing Zhang, Yong Feng, Qian Tang, Zhen-Zhong Zhu","doi":"10.1016/j.biomaterials.2024.123001","DOIUrl":"10.1016/j.biomaterials.2024.123001","url":null,"abstract":"<p><p>Necrosis at the distal end of random skin flaps remains a significant challenge, limiting the clinical application of these flaps in plastic and reconstructive surgery. Inhibiting ischemia/reperfusion (I/R) injury and promoting the formation of neovascular networks are critical preventive strategies. Platelet-derived small extracellular vesicles (PL-sEV) are nanocarriers of growth factors that provide an alternative to clinically used platelet-rich plasma and platelet lysates, offering higher growth factor concentrations and lower immunogenicity. In this study, PANoptosis, a distinct form of inflammatory cell death, was fully characterized in a random skin flap model. Subcutaneous injection of PL-sEV improved ischemic skin flap survival by enhancing blood perfusion and reducing PANoptosis levels. In vitro, PL-sEV inhibited oxygen-glucose deprivation/reoxygenation-induced dysfunction in human umbilical vein endothelial cells. Furthermore, PL-sEV was incorporated into a thermosensitive triblock hydrogel, creating a sprayable delivery system (PLEL@PL-sEV). Mechanistic analysis through RNA sequencing indicated that the protective effects of PL-sEV against PANoptosis likely resulted from its anti-inflammatory properties, particularly via suppression of the NF-κB signaling pathway. This novel hydrogel system demonstrated controlled release of PL-sEV and proved effective in improving skin flap transplantation outcomes.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123001"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-12-19DOI: 10.1016/j.biomaterials.2024.123036
Ying Gao, Shi Yin, Ying Guo, Guoyin Chen, Yanhong Wei, Jialei Yang, Hongyan Chen, Kai Hou, Meifang Zhu
{"title":"Hydrogel-based nonwoven with persistent porosity for whole-stage hypertonic wound healing by regulating of water vaporization enthalpy.","authors":"Ying Gao, Shi Yin, Ying Guo, Guoyin Chen, Yanhong Wei, Jialei Yang, Hongyan Chen, Kai Hou, Meifang Zhu","doi":"10.1016/j.biomaterials.2024.123036","DOIUrl":"10.1016/j.biomaterials.2024.123036","url":null,"abstract":"<p><p>Moisture induced by wound exudate is crucial throughout the wound repair process. The dressing directly affects the absorption, permeation, and evaporation of the wound exudate. However, most dressings in clinical often result in excessive dryness or moisture of wound due to their monotonous structure and function, leading to ineffective thermodynamic control of evaporation enthalpy. Herein, a hydrogel-based nonwoven dressing (Gel-Fabric) with asymmetric amphiphilic surface and persistent microscopic porous structure is constructed by integrating intrinsic hydrophilic absorbent hydrogel fibers and hydrophobic ultrafine PET fibers. The novel Gel-Fabric facilitates rapid vertical drainage of wound exudate through the capillary effect and Laplace pressure synergy. Additionally, dynamic stepwise moisture management is also achieved by regulating the vaporization enthalpy of exudate. In vivo experiments confirm that Gel-Fabric significantly promotes wound healing, vascularization, and endothelialization, achieving a higher healing rate than ordinary dressings. Furthermore, compared to the clinical dressings, Gel-Fabric significantly reduces the frequency of dressing changes, offering improved outcomes for patients and more efficient wound management for healthcare providers.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123036"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-11-26DOI: 10.1016/j.biomaterials.2024.122974
Liang Wu, Tao Xu, Sen Li, Kai Sun, Ziyang Tang, Hui Xu, Yong Qiu, Zhenhua Feng, Zhen Liu, Zezhang Zhu, Xiaodong Qin
{"title":"Sequential activation of osteogenic microenvironment via composite peptide-modified microfluidic microspheres for promoting bone regeneration.","authors":"Liang Wu, Tao Xu, Sen Li, Kai Sun, Ziyang Tang, Hui Xu, Yong Qiu, Zhenhua Feng, Zhen Liu, Zezhang Zhu, Xiaodong Qin","doi":"10.1016/j.biomaterials.2024.122974","DOIUrl":"10.1016/j.biomaterials.2024.122974","url":null,"abstract":"<p><p>The osteogenic microenvironment (OME) significantly influences bone repair; however, reproducing its dynamic activation and repair processes remains challenging. In this study, we designed injectable porous microspheres modified with composite peptides to investigate cascade alterations in OME and their underlying mechanisms. Poly <sub>l</sub>-lactic acid microfluidic microspheres underwent surface modifications through alkaline hydrolysis treatment, involving heterogeneous grafting of bovine serum albumin nanoparticles with stem cell-homing peptides (BNP@SKP) and BMP-2 mimicking peptides (P24), respectively. These modifications well-organized the actions of initial release and subsequent in situ grafting of peptides. Cellular experiments demonstrated varied degrees of chemotactic recruitment and osteogenic differentiation in mesenchymal stem cells. Further biological analysis revealed that BNP@SKP targeted the Ras/Erk axis and upregulated matrix metalloproteinase (MMP)2 and MMP9 expression, thereby enhancing initial chemotaxis and recruitment. In vivo studies validated the establishment of a dynamically regulated OME centered on the microspheres, resulting in increased stem cell recruitment, sequential activation of the differentiation microenvironment, and facilitation of in situ osteogenesis without ectopic ossification. In conclusion, this study successfully fabricated composite peptide-modified microspheres and systematically explored the mechanisms of bone formation through sequential activation of OME via heterogeneous grafting of signaling molecules. This provides theoretical evidence for biomaterials based on microenvironment regulation.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"122974"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulating tumor cells to awaken T cell antitumor function and enhance melanoma immunotherapy.","authors":"Weihan Zhang, Shijun Yuan, Zipeng Zhang, Shunli Fu, Shujun Liu, Jinhu Liu, Qingping Ma, Zhenxing Xia, Panpan Gu, Shuying Gao, Zhiyue Zhang, Xinke Zhang, Yongjun Liu, Na Zhang","doi":"10.1016/j.biomaterials.2024.123034","DOIUrl":"10.1016/j.biomaterials.2024.123034","url":null,"abstract":"<p><p>Tumor cells transmit various immunosuppressive signals and induce a dysfunctional state in T cells, which essentially leads to immune escape and tumor progression. However, developing effective strategies to counteract the domestication of T cells by tumor cells remains a challenge. Here, we prepared pH-responsive lipid nanoparticles (NL/PLDs) co-loaded with PCSK9 shRNA, lonidamine (LND), and low-dose doxorubicin (DOX). NL/PLDs can awaken domesticated T cells function by sending pro-activation, pro-recognition, and pro-killing signals by increasing tumor immunogenicity, increasing the expression of major histocompatibility complex I (MHC-I) on tumor cells, and alleviating the suppression effect of tumor-secreted lactic acid (LA) on the T cell effector function, respectively. In melanoma-bearing mice, NL/PLDs effectively relieved tumor immunosuppressive microenvironment (TIME) and enhanced the antitumor immunity mediated by CD8<sup>+</sup> T cells. Furthermore, when combined with aPD-1, NL/PLDs demonstrated strong antitumor effects and increased immunotherapeutic efficacy. This regulatory strategy provides new insights for enhancing immunotherapy by regulating tumor immunosuppressive signals and shows significant potential for clinical tumor treatment.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123034"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-12-10DOI: 10.1016/j.biomaterials.2024.122996
Nasim Annabi, Devyesh Rana, Ehsan Shirzaei Sani, Roberto Portillo-Lara, Jessie L Gifford, Mohammad M Fares, Suzanne M Mithieux, Anthony S Weiss
{"title":"Corrigendum to \"Engineering a sprayable and elastic hydrogel adhesive with antimicrobial properties for wound healing\" [Biomaterials 139 (2017), 18080].","authors":"Nasim Annabi, Devyesh Rana, Ehsan Shirzaei Sani, Roberto Portillo-Lara, Jessie L Gifford, Mohammad M Fares, Suzanne M Mithieux, Anthony S Weiss","doi":"10.1016/j.biomaterials.2024.122996","DOIUrl":"10.1016/j.biomaterials.2024.122996","url":null,"abstract":"","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":" ","pages":"122996"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiomaterialsPub Date : 2025-05-01Epub Date: 2024-12-10DOI: 10.1016/j.biomaterials.2024.123002
Xiaoyu Li, Chao Xia, Zhaokui Jin, Qianjun He
{"title":"Ascorbate/methionine-based CH<sub>4</sub> delivery nanomedicine for tumor-targeted therapy.","authors":"Xiaoyu Li, Chao Xia, Zhaokui Jin, Qianjun He","doi":"10.1016/j.biomaterials.2024.123002","DOIUrl":"10.1016/j.biomaterials.2024.123002","url":null,"abstract":"<p><p>Methane (CH<sub>4</sub>) is identified to be an emerging anti-inflammation and anti-cancer molecule with high bio-safety, but the targeted delivery of CH<sub>4</sub> is a thorny challenge. Herein, we propose a CH<sub>4</sub> delivery strategy based on an intratumoral H<sub>2</sub>O<sub>2</sub>-triggered cascade reaction of ascorbic acid (AA)/methionine (Met), and have constructed a new nanomedicine (AMN) for tumor-targeted CH<sub>4</sub> therapy. Encouragingly, AMN realizes the effective tumor-targeted delivery and intratumoral H<sub>2</sub>O<sub>2</sub>-responsive release of CH<sub>4</sub>, and exhibits significant anti-cancer effects and high bio-safety. Mechanistically, we have discovered that intratumoral released CH<sub>4</sub> can not only induce the apoptosis of 4T1 tumor cells by inhibiting their mitochondrial metabolism, but also activate tumor immunotherapy by reprogramming tumor-associated macrophages (TAMs) phenotype (M2 to M1). The combination of the above anti-cancer pathways by virtue of tumor-targeted CH<sub>4</sub> delivery makes contribution to outstanding anti-cancer efficacy of AMN. The proposed CH<sub>4</sub> delivery strategy opens a new window for safe and effective tumor therapy.</p>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"316 ","pages":"123002"},"PeriodicalIF":12.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}