Biomaterials最新文献

{"title":"Targeting myeloid cells with platelet-derived extracellular vesicles to overcome resistance of immune checkpoint blockade therapy","authors":"Chenlu Yao ,&nbsp;Qingle Ma ,&nbsp;Heng Wang ,&nbsp;Bingbing Wu ,&nbsp;Huaxing Dai ,&nbsp;Jialu Xu ,&nbsp;Jinyu Bai ,&nbsp;Fang Xu ,&nbsp;Admire Dube ,&nbsp;Chao Wang","doi":"10.1016/j.biomaterials.2025.123336","DOIUrl":"10.1016/j.biomaterials.2025.123336","url":null,"abstract":"<div><div>Immune checkpoint blockade (ICB) therapy is designed to boost antitumor immune responses, yet it may unintentionally alter the chemokine profile, which can attract suppressive myeloid cells to the tumor, leading to acquired immune resistance. To address this, we developed a platform that targets myeloid cells post-ICB therapy using platelet-derived extracellular vesicles (PEVs). Unlike free drug administration, this system selectively targets anti-PD-L1-treated tumors through the CXCL-CXCR2 axis, effectively redirecting myeloid cells and overcoming ICB resistance. Consequently, mice exhibited robust responses to subsequent ICB therapy cycles, resulting in significantly enhanced tumor clearance and prolonged survival. The PEVs’ targeting capability was also effective in tumors treated with chemotherapy and radiotherapy, suggesting a wide range of potential applications. In summary, PEVs offer a versatile platform for targeted immunomodulation to counteract acquired immune resistance during ICB therapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123336"},"PeriodicalIF":12.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine","authors":"Hongjian Tian ,&nbsp;Wenfeng Zeng ,&nbsp;Zihao Wang ,&nbsp;Siqi Li ,&nbsp;Wenjing Wei ,&nbsp;Shanshan Li ,&nbsp;Xiaozhe Yin ,&nbsp;Wenjing Na ,&nbsp;Youwang Wang ,&nbsp;Kai Song ,&nbsp;Ping Zhu ,&nbsp;Wei Liang","doi":"10.1016/j.biomaterials.2025.123333","DOIUrl":"10.1016/j.biomaterials.2025.123333","url":null,"abstract":"<div><div>The clinical benefits of personalized therapeutic tumor vaccines are mainly challenged by the need to identify immunogenic neoantigens promptly, given the rapid pace of tumor mutations. An increasing body of literature addresses the potential of tumor-derived extracellular vesicles (TEVs) as an anti-tumor “cell-free” vaccine due to their substantial presence of neoantigens. However, their immunosuppression and limited presentation efficiency of dendritic cells (DCs) restrict their further application. Here, we have developed a novel tumor-personalized vaccine, termed P-Pev, based on remodeled TEVs by polymeric surfactant polyethylene glycol-phosphatidyleolamine (PEG-PE) and adjuvant monophosphoryl lipid A (MPLA). Our results show that PEG-PE transforms TEVs into micelle-like complexes by disrupting the original structure, facilitating antigens delivery to the cytoplasm, and cross-presentation by DCs. P-Pev particularly prevents the immunosuppressive impacts of TEVs on the ability of DCs to prime CD8⁺ T cells and eliminates the potency of TEVs to promote lung metastasis through their membrane-bound PD-L1. Finally, the P-Pev effectively induces neoantigen-specific cytotoxic T lymphocytes (CTLs) responses and exhibits excellent therapeutic effects in various murine tumor models. Also, the P-Pev induces neoantigen-specific antibodies, suggesting the involvement of humoral immunity in its anti-tumor effects. More importantly, it has been shown that P-Pev prepared by mutated tumor cells can retard these mutated tumor cell-established syngeneic tumors better than P-Pev prepared by original tumor cells, indicating the feasibility that leverages TEVs to prepare personalized tumor vaccines, and it is synergistically enhanced by PD-1 mAb combination. Collectively, we present a general strategy that offers a streamlined, cost-effective, and time-consuming approach to preparing personalized therapeutic tumor vaccines.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123333"},"PeriodicalIF":12.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-activated sonothermal-catalytic synergistic therapy via asymmetric electron distribution for bacterial wound infections","authors":"Ye Qi ,&nbsp;Shuangsong Ren ,&nbsp;Xiaolong Ou ,&nbsp;Pisong Li ,&nbsp;Han Wu ,&nbsp;Ying Che ,&nbsp;Xinyi Wang","doi":"10.1016/j.biomaterials.2025.123338","DOIUrl":"10.1016/j.biomaterials.2025.123338","url":null,"abstract":"<div><div>Antibiotic-resistant bacterial infections present a growing global health challenge, requiring innovative therapeutic solutions to overcome current limitations. We introduce boron-integrated bismuth oxide (B–BiO₂) nanosheets with an asymmetrically distributed electronic structure for ultrasound-activated synergistic sonothermal and catalytic therapy. Boron incorporation enhances local electron density distribution, optimizing charge separation and significantly improving sonothermal and catalytic efficiency, as validated by density functional theory calculations. These nanosheets exhibit dual functionality, effectively generating localized heat and reactive oxygen species (ROS) under ultrasound, leading to 99.999 % antibacterial efficacy against multidrug-resistant pathogens by disrupting bacterial membranes, as demonstrated through all-atom simulations and <em>in vitro</em> experiments. The simulations further reveal that sonothermal conversion effects enhance bacterial membrane fluidity and induce structural defects, amplifying ROS-induced oxidative damage and membrane destabilization. <em>In vivo</em>, B–BiO₂ nanosheets accelerate wound healing in methicillin-resistant <em>Staphylococcus aureus</em> (MRSA)-infected murine models, achieving 99.8 % closure by day 14 by reducing inflammation and promoting angiogenesis and tissue regeneration. Transcriptomic analysis highlights the activation of extracellular matrix remodeling, angiogenesis, and autophagy pathways, underscoring the nanosheets’ therapeutic potential. This study establishes ultrasound-activated B–BiO₂ nanosheets as a novel nanotherapeutic platform, leveraging asymmetric electron distribution to synergistically combat drug-resistant infections and promote effective wound healing.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123338"},"PeriodicalIF":12.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming radiation-induced PD-L1 and COX-2 upregulation by nitric oxide gas nanogenerator to sensitize radiotherapy of lung cancer","authors":"Rui Hu ,&nbsp;Xin Jiang ,&nbsp;Lijie Zhu ,&nbsp;Rui Meng ,&nbsp;Rongbo Yang ,&nbsp;Wenjie Sun ,&nbsp;Zhenzhou Zhao ,&nbsp;Yuehua Lyu ,&nbsp;Ruoyuan Huang ,&nbsp;Fei Xue ,&nbsp;Mengke Shi ,&nbsp;Zaigang Zhou ,&nbsp;Jianliang Shen ,&nbsp;Congying Xie","doi":"10.1016/j.biomaterials.2025.123335","DOIUrl":"10.1016/j.biomaterials.2025.123335","url":null,"abstract":"<div><div>Currently, certain lung cancer patients exhibit resistance to radiotherapy due to reduced DNA damage under hypoxic conditions and the cytoprotective and immune-resistance effect caused by increased programmed death ligand-1 (PD-L1) and Cyclooxygenase 2 (COX-2) expression after radiotherapy. At present, existing nanoparticles or drugs could hardly effectively, and easily address these obstacles faced by highly effective radiotherapy simultaneously, especially the simultaneous depression of PD-L1 and COX-2. In this study, it is newly proved that some typical nitric oxide (NO) gas donors could co-inhibit PD-L1 and COX-2 expression, revealing the possible not fully proven role of NO in reversing tumor immunotherapy resistance. Then, to realize selective NO generation in tumors, a simple tumor glutathione (GSH) responsive NO gas nanogenerator named SAB-NO nanoparticles was designed and prepared, which was composed of the NO donor Isoamyl Nitrite conjugated with serum albumin (SAB). By doing this, SAB-NO nanoparticles more effectively sensitized radiotherapy through breaking the cytoprotective effects faced by radiotherapy <em>in vitro</em> by generating more DNA damage through reversing tumor hypoxia and impairing the DNA damage repair process through decreasing PD-L1 expression. Then, the combination therapy of SAB-NO nanoparticles and radiotherapy effectively transformed cold tumors into hot ones through avoiding some potential immune-resistance effects induced by radiotherapy treatment alone through PD-L1 and COX-2 co-inhibition. In conclusion, the combined treatment of radiotherapy and SAB-NO nanoparticles finally almost completely suppressed the growth of lung tumors, revealing the novel role of NO donors in sensitizing tumor immunotherapy by avoiding the potential cytoprotective and immune-resistance effects.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123335"},"PeriodicalIF":12.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elastic sac-shaped hydrogel dressing with responsive antibacterial and pro-healing in movable wounds via MOF activated ink spraying","authors":"Mingxin Qiao ,&nbsp;Bin Cheng ,&nbsp;Weimin Wu ,&nbsp;Yanhua Liu ,&nbsp;Jian Wang ,&nbsp;Xibo Pei ,&nbsp;Zhou Zhu ,&nbsp;Qianbing Wan","doi":"10.1016/j.biomaterials.2025.123318","DOIUrl":"10.1016/j.biomaterials.2025.123318","url":null,"abstract":"<div><div>In daily life, sports frequently cause skin injuries, particularly in movable parts such as joints. However, the frequent movement of joints can impede the proper fitting of dressings, resulting in re-tearing of the wound, an increased infection risk, and prolonged healing. Moreover, demand for skin wound dressings in movable parts has risen, as around 2.4 million joint surgeries are performed annually. Therefore, it is crucial to design an elastic wound dressing that can accommodate repeated joint movements and control wound infection responsively. In this study, a biomimetic hydrogel dressing was designed based on the inkjet behaviour of the elastic ink sac of cuttlefish through repeated extrusion. This dressing comprises a highly elastic polyether F127 diacrylate-based ink sac with micro-nozzles, along with antibacterial and pro-healing ink, metal-organic framework modified gelatin, possessing responsive release properties. With the movement rhythm, the super-elastic dressing perfectly conforms to the wounds in joints or other movable parts to absorb exudation and release therapeutic ink in response to the microenvironment to prevent infection. In conclusion, the biomimetic dressing demonstrates excellent mechanical properties with a deformation of approximately 400 %, and attains an antibacterial rate exceeding 95 %. Compared with the control group, collagen production increases by 2.6 times, and the wound healing speed is enhanced by over 20 %. Therefore, the application of the biomimetic dressing is anticipated to offer a novel approach for managing skin infection wounds in movable parts.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123318"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seeds-and-soil inspired hydrogel microspheres: A dual-action antioxidant and cellular therapy for reversing intervertebral disc degeneration","authors":"Yilin Yang ,&nbsp;Jiangbo Guo ,&nbsp;Haifei Cao ,&nbsp;Xin Tian ,&nbsp;Hao Shen ,&nbsp;Junjie Niu ,&nbsp;Huilin Yang ,&nbsp;Qin Shi ,&nbsp;Yong Xu","doi":"10.1016/j.biomaterials.2025.123326","DOIUrl":"10.1016/j.biomaterials.2025.123326","url":null,"abstract":"<div><div>Intervertebral disc degeneration (IVDD) is a globally prevalent disease, yet achieving dual repair of tissue and function presents significant challenges. Considering reactive oxygen species (ROS) is a primary cause of IVDD, and given the decrease of nucleus pulposus cells (NPCs) and extensive degradation of extracellular matrix (ECM) during IVDD development, the present study, inspired by the “seeds-and-soil” strategy, has developed NPCs-loaded TBA@Gel&amp;Chs hydrogel microspheres. These microspheres serve as exogenous supplements of NPCs and ECM analogs, replenishing “seeds” and “soil” for nucleus pulposus repair, and incorporating polyphenol antioxidant components to interrupt the oxidative stress-IVDD cycle, thereby constructing a microsphere system where NPCs and ECM support each other. Experiments proved that TBA@Gel&amp;Chs exhibited significant extracellular ROS-scavenging antioxidant capabilities while effectively upregulating intracellular antioxidant proteins expression (Sirt3 and Sod2). This dual-action antioxidant capability effectively protects the vitality and physiological functions of NPCs. The therapeutic effects of microspheres on IVDD were also confirmed in rat models, which was found significantly restore histological structure and mechanical properties of degenerated discs. Additionally, RNA-seq results have provided evidences of antioxidant mechanism by which TBA@Gel&amp;Chs protected NPCs from oxidative stress. Therefore, the NPCs-loaded TBA@Gel&amp;Chs microspheres developed in this study have achieved excellent therapeutic effects, offering a paradigm using antioxidant biomaterials combined with cellular therapy for IVDD treatment.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123326"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal ion coordinated tea polyphenol nanocoating for enhanced probiotic therapy in inflammatory bowel disease","authors":"Lu Gao ,&nbsp;Yunjian Liu ,&nbsp;Ling Ye ,&nbsp;Sizhi Liang ,&nbsp;Jiancan Lin ,&nbsp;Jiaying Zeng ,&nbsp;Lei Lei ,&nbsp;Qiang Huang ,&nbsp;Yujun Wan ,&nbsp;Bin Zhang","doi":"10.1016/j.biomaterials.2025.123323","DOIUrl":"10.1016/j.biomaterials.2025.123323","url":null,"abstract":"<div><div>Probiotics encapsulated with metal-phenolic networks (MPNs) present a promising approach for treating inflammatory bowel diseases (IBD). However, current MPN systems predominantly use tannic acid (TA) as the phenolic source, with limited exploration of other polyphenols, and face challenges in long-term stability and biocompatibility. Herein, three alternative tea polyphenols, gallic acid (GA), epigallocatechin (EGC) and epigallocatechin gallate (EGCG), were coordinated with ferric ions, to fabricate MPN-coated <em>Lactobacillus rhamnosus</em> LGG (MPN@L). These were compared with TA-based MPN@L to evaluate their effectiveness in alleviating IBD. All MPN@L complexes demonstrated superior adhesion and retention compared to uncoated probiotics in both <em>ex vivo</em> and <em>in vivo</em> models. Specifically, EGC@L exhibited the highest survival rate throughout gastrointestinal digestion, with a 2.7 log CFU/mL improvement over uncoated probiotics, and showed optimal retention in murine intestine with a fluorescence intensity of 24.3 × 10⁶ p/s/cm²/sr by day four. All MPN@L formation effectively alleviated ulcerative colitis by reducing myeloperoxidase levels, modulating cytokines profiles, and enhancing gut microbiota. EGC@L particularly increased beneficial bacterial genera, including <em>Lactobacillus</em>, <em>Adlercreutzia</em>, and <em>Oscillospira</em>, while decreasing the pro-inflammatory genera. This study highlights the potential of MPN-based probiotic microencapsulation to enhanced treatment for gastrointestinal disorders, expending the application of probiotic microencapsulation in IBD therapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123323"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gelatin sponge patch grafting of microcryogel-based three-dimensional mesenchymal stem cells to alleviate acute liver failure","authors":"Haimeng Song ,&nbsp;Xinyue Du ,&nbsp;Yuanyuan Zhang ,&nbsp;Wei Liu ,&nbsp;Yi Luo ,&nbsp;Yuxin Liu ,&nbsp;Yongjia Xue ,&nbsp;Mingyang Xu ,&nbsp;Jizhen Lu ,&nbsp;Wenwen Jia ,&nbsp;Yanan Du ,&nbsp;Lining Cao ,&nbsp;Jianfeng Lu ,&nbsp;Wencheng Zhang ,&nbsp;Zhiying He","doi":"10.1016/j.biomaterials.2025.123324","DOIUrl":"10.1016/j.biomaterials.2025.123324","url":null,"abstract":"<div><div>The clinical application of human umbilical cord mesenchymal stem cells (hUCMSCs) in the treatment of liver failure faces challenges due to cell quality, short engraftment time, and limited efficacy. Here, gelatin microcryogel (GM) microcarriers with pore sizes ranging from 15 to 36 μm were tuned from mixed gelatin and glutaraldehyde to develop a 3D culture system of hUCMSCs with improved therapeutic effects. Bulk RNA sequencing and <em>in vitro</em> assays showed that 3D-hUCMSCs exhibited significant improvement in signaling pathways related to paracrine secretion and anti-inflammation. These 3D-hUCMSCs superior compared to 2D-hUCMSCs not only in terms of paracrine secretion, protection from oxidation, and resistance to mechanical force damage, but also had better liver function improvement effect than 2D-hUCMSCs when they were transplanted as single cells into liver injury mice. Furthermore, a gelatin sponge patch grafting (GSPG) strategy was developed to enable the direct engraftment of 3D-hUCMSCs within the GM microcarriers. The results showed that overall engraftment in the host liver was significantly improved, and the life span of transplanted hosts was extended. Our study provided a practical strategy to achieve high engraftment and long retraining time of 3D-hUCMSCs in rescuing acute liver failure with gelatin matrixes.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123324"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive ultrasonic debridement of implant biofilms via hydrogen-sulfide releasing peptide nanoemulsions","authors":"Harminder Singh ,&nbsp;Diptomit Biswas ,&nbsp;Ji Ho Park ,&nbsp;Mary E. Landmesser ,&nbsp;Dino J. Ravnic ,&nbsp;Scott H. Medina","doi":"10.1016/j.biomaterials.2025.123337","DOIUrl":"10.1016/j.biomaterials.2025.123337","url":null,"abstract":"<div><div>Implant contamination by bacterial biofilms remains a significant healthcare burden, often necessitating revision surgeries due to biofilm-enabled antibiotic resistance. Physical debridement, in combination with chemical antiseptics, is a simple and effective therapeutic strategy, but requires highly invasive surgical procedures and risks secondary infection events. Herein, we report a non-invasive, nanoparticle-enabled ultrasonic debridement strategy that exerts synergistic physical and chemical antiseptic effects to rapidly and efficiently clear implant-associated biofilms <em>in situ</em>. This approach is realized through the development of hydrogen sulfide releasing peptide nanoemulsions that preferentially target bacterial biofilms and can be vaporized via diagnostic ultrasound to spatiotemporally clear methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) infections. Biophysical studies elucidate the mechanistic basis for the platform's anti-biofilm activity, and <em>in vitro</em>, <em>ex vivo</em> and <em>in vivo</em> experiments confirm efficacy in the context of MRSA-infected titanium implants. By exploiting the portable, low cost and safe nature of low intensity diagnostic ultrasound, this non-invasive approach avoids the collateral tissue damage associated with current surgical and high intensity acoustic ablative modalities.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123337"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose-triggered NO-evolving coating bestows orthopedic implants with enhanced anti-bacteria and angiectasis for safeguarding diabetic osseointegration","authors":"Hongxing Shi ,&nbsp;Hao Yang ,&nbsp;Chao Wu ,&nbsp;Song Wang ,&nbsp;Shuai He ,&nbsp;Lin Chen ,&nbsp;Yau Kei Chan ,&nbsp;Shuangquan Lai ,&nbsp;Kunneng Liang ,&nbsp;Yi Deng","doi":"10.1016/j.biomaterials.2025.123334","DOIUrl":"10.1016/j.biomaterials.2025.123334","url":null,"abstract":"<div><div>As a common chronic metabolic disease, diabetes mellitus (DM) features a hyperglycemic micromilieu around implants, resulting in the critical implantation failure and high complications such as peri-implantitis and angiectasis disorder. To address the plaguing issue, we devise and develop a glucose-unlocked NO-evolving orthopedic implant consisted of polyetheretherketone (PEEK), glucose oxidase (GOx) and <span>l</span>-arginine (Arg) with enhanced angiogenesis for boosting diabetic osseointegration. Upon hyperglycemic niche, GOx on implants catalytically exhaust glucose to H₂O₂, which immediately reacts with Arg to <em>in situ</em> liberate nitric oxide (NO), resulting in enhanced angiogenesis and angiectasis around PEEK implant. Besides, the engineered implant exhibits great anti-bacterial properties against both Gram-positive and Gram-negative bacteria, as well as fortifies osteogenicity of osteoblasts in terms of cell proliferation, alkaline phosphatase activity and calcium matrix mineralization. Intriguingly, <em>in vivo</em> evaluations utilizing diabetic infectious bone defect models of rat further authenticate that the engineered implants substantially augment bone remodeling and osseointegration at weeks 4 and 8 through dampening pathogens, anti-inflammatory as well as promoting angiectasis. Altogether, this work proposed a new tactic to remedy stalled diabetic osseointegration with hyperglycemic micromilieu-responsive therapeutic gas-evolving orthopedic implants.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123334"},"PeriodicalIF":12.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}