Plant Biotechnology Journal最新文献

Xiaoshu, a simple genetic model system for sweetpotato (Ipomoea batatas (L.) Lam.)

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-12-02 DOI: 10.1111/pbi.14528

Shizhuo Xiao, Yao Wang, Zhilin Zhou, Lingxiao Zhao, Lukuan Zhao, Bingqian Gao, Xibin Dai, Pan Xu, Qinghe Cao

引用次数: 0

Development of vegetative oil sorghum: From lab‐to‐field

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-30 DOI: 10.1111/pbi.14527

Kiyoul Park, Truyen Quach, Teresa J. Clark, Hyojin Kim, Tieling Zhang, Mengyuan Wang, Ming Guo, Shirley Sato, Tara J. Nazarenus, Rostislav Blume, Yaroslav Blume, Chi Zhang, Stephen P. Moose, Kankshita Swaminathan, Jörg Schwender, Thomas Elmo Clemente, Edgar B. Cahoon

{"title":"Development of vegetative oil sorghum: From lab‐to‐field","authors":"Kiyoul Park, Truyen Quach, Teresa J. Clark, Hyojin Kim, Tieling Zhang, Mengyuan Wang, Ming Guo, Shirley Sato, Tara J. Nazarenus, Rostislav Blume, Yaroslav Blume, Chi Zhang, Stephen P. Moose, Kankshita Swaminathan, Jörg Schwender, Thomas Elmo Clemente, Edgar B. Cahoon","doi":"10.1111/pbi.14527","DOIUrl":"https://doi.org/10.1111/pbi.14527","url":null,"abstract":"SummaryBiomass crops engineered to accumulate energy‐dense triacylglycerols (TAG or ‘vegetable oils’) in their vegetative tissues have emerged as potential feedstocks to meet the growing demand for renewable diesel and sustainable aviation fuel (SAF). Unlike oil palm and oilseed crops, the current commercial sources of TAG, vegetative tissues, such as leaves and stems, only transiently accumulate TAG. In this report, we used grain (Texas430 or TX430) and sugar‐accumulating ‘sweet’ (Ramada) genotypes of sorghum, a high‐yielding, environmentally resilient biomass crop, to accumulate TAG in leaves and stems. We initially tested several gene combinations for a ‘push‐pull‐protect’ strategy. The top TAG‐yielding constructs contained five oil transgenes for a sorghum WRINKLED1 transcription factor (‘push’), a Cuphea viscosissima diacylglycerol acyltransferase (DGAT; ‘pull’), a modified sesame oleosin (‘protect’) and two combinations of specialized Cuphea lysophosphatidic acid acyltransferases and medium‐chain acyl‐acyl carrier protein thioesterases. Though intended to generate oils with medium‐chain fatty acids, engineered lines accumulated oleic acid‐rich oil to amounts of up to 2.5% DW in leaves and 2.0% DW in stems in the greenhouse, 36‐fold and 49‐fold increases relative to wild‐type (WT) plants, respectively. Under field conditions, the top‐performing event accumulated TAG to amount to 5.5% DW in leaves and 3.5% DW in stems, 78‐fold and 58‐fold increases, respectively, relative to WT TX430. Transcriptomic and fluxomic analyses revealed potential bottlenecks for increased TAG accumulation. Overall, our studies highlight the utility of a lab‐to‐field pipeline coupled with systems biology studies to deliver high vegetative oil sorghum for SAF and renewable diesel production.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"08 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Past innovations and future possibilities in plant chromosome engineering

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-29 DOI: 10.1111/pbi.14530

Yang Liu, Qian Liu, Congyang Yi, Chang Liu, Qinghua Shi, Mian Wang, Fangpu Han

{"title":"Past innovations and future possibilities in plant chromosome engineering","authors":"Yang Liu, Qian Liu, Congyang Yi, Chang Liu, Qinghua Shi, Mian Wang, Fangpu Han","doi":"10.1111/pbi.14530","DOIUrl":"https://doi.org/10.1111/pbi.14530","url":null,"abstract":"Plant chromosome engineering has emerged as a pivotal tool in modern plant breeding, facilitating the transfer of desirable traits through the incorporation of alien chromosome fragments into plants. Here, we provide a comprehensive overview of the past achievements, current methodologies and future prospects of plant chromosome engineering. We begin by examining the successful integration of specific examples such as the incorporation of rye chromosome segments (e.g. the 1BL/1RS translocation), Dasypyrum villosum segments (e.g. the 6VS segment for powdery mildew resistance), Thinopyrum intermedium segments (e.g. rust resistance genes) and Thinopyrum elongatum segments (e.g. Fusarium head blight resistance genes). In addition to trait transfer, advancements in plant centromere engineering have opened new possibilities for chromosomal manipulation. This includes the development of plant minichromosomes via centromere-mediated techniques, the generation of haploids through CENH3 gene editing, and the induction of aneuploidy using KaryoCreate. The advent of CRISPR/Cas technology has further revolutionized chromosome engineering, enabling large-scale chromosomal rearrangements, such as inversions and translocations, as well as enabling targeted insertion of large DNA fragments and increasing genetic recombination frequency. These advancements have significantly expanded the toolkit for genetic improvement in plants, opening new horizons for the future of plant breeding.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142742781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single‐cell transcriptomic profiling of maize cell heterogeneity and systemic immune responses against Puccinia polysora Underw

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-29 DOI: 10.1111/pbi.14519

Xiao‐Cui Yan, Qing Liu, Qian Yang, Kai‐Lai Wang, Xiu‐Zhen Zhai, Meng‐Yun Kou, Jia‐Long Liu, Shang‐Tong Li, Shu‐Han Deng, Miao‐Miao Li, Hui‐Jun Duan

{"title":"Single‐cell transcriptomic profiling of maize cell heterogeneity and systemic immune responses against Puccinia polysora Underw","authors":"Xiao‐Cui Yan, Qing Liu, Qian Yang, Kai‐Lai Wang, Xiu‐Zhen Zhai, Meng‐Yun Kou, Jia‐Long Liu, Shang‐Tong Li, Shu‐Han Deng, Miao‐Miao Li, Hui‐Jun Duan","doi":"10.1111/pbi.14519","DOIUrl":"https://doi.org/10.1111/pbi.14519","url":null,"abstract":"SummarySouthern corn rust (SCR), caused by Puccinia polysora Underw (P. polysora), is a catastrophic disease affecting maize, leading to significant global yield losses. The disease manifests primarily as pustules on the upper surface of corn leaves, obscuring our understanding of its cellular heterogeneity, the maize's response to its infection and the underlying gene expression regulatory mechanisms. In this study, we dissected the heterogeneity of maize's response to P. polysora infection using single‐cell RNA sequencing. We delineated cell‐type‐specific gene expression alterations in six leaf cell types, creating the inaugural single‐cell atlas of a maize leaf under fungal assault. Crucially, by reconstructing cellular trajectories in susceptible line N110 and resistant line R99 during infection, we identified diverse regulatory programs that fortify R99's resistance across different leaf cell types. This research uncovers an immune‐like state in R99 leaves, characterized by the expression of various fungi‐induced genes in the absence of fungal infection, particularly in guard and epidermal cells. Our findings also highlight the role of the fungi‐induced glycoside hydrolase family 18 chitinase 7 protein (ZmChit7) in conferring resistance to P. polysora. Collectively, our results shed light on the mechanisms of maize resistance to fungal pathogens through comparative single‐cell transcriptomics, offering a valuable resource for pinpointing novel genes that bolster resistance to P. polysora.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"27 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsSPL5 promotes rice outcrossing efficiency by G-protein pathway.

IF 10.1 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-29 DOI: 10.1111/pbi.14514

Fangping Li, Quanya Tan, Zhenpeng Gan, Danlu Han, Weifeng Yang, Xin Luan, Jieying Liu, Hongyuan Zhao, Yu Fu, Shu Wang, Haifei Hu, Shiqiang Xu, Junliang Zhao, Haitao Zhu, Zupei Liu, Chengwei Yang, Xiangdong Fu, Guiquan Zhang, Shaokui Wang

引用次数: 0

Spatiotemporal regulation of anther's tapetum degeneration paved the way for a reversible male sterility system in cotton. 花药锥体退化的时空调控为棉花的可逆雄性不育系统铺平了道路。

IF 10.1 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-28 DOI: 10.1111/pbi.14518

Rishi Kumar Verma, Surendra Pratap Singh, Sudhir Pratap Singh, Shiv Narayan, Praveen C Verma, Samir V Sawant

{"title":"Spatiotemporal regulation of anther's tapetum degeneration paved the way for a reversible male sterility system in cotton.","authors":"Rishi Kumar Verma, Surendra Pratap Singh, Sudhir Pratap Singh, Shiv Narayan, Praveen C Verma, Samir V Sawant","doi":"10.1111/pbi.14518","DOIUrl":"https://doi.org/10.1111/pbi.14518","url":null,"abstract":"Male sterility is an important agronomical trait in self-pollinating plants for producing cost-effective F1 hybrids to harness the heterosis. Still, large-scale development and maintenance of male sterile lines and restoring fertility in F1 hybrids pose significant challenges in plant hybrid breeding. Cotton is a self-pollinating crop and exhibits strong hybrid vigor. However, there are currently few breeding methods to achieve cost-effective production of F1 hybrid cotton. Here, we utilized novel functions of the Arabidopsis autophagy-related BECLIN1/ATG6 and a mutant of E3 ubiquitin ligase COP1 (COP1L105A) genes in developing rescuable male sterility in cotton. We have generated multiple male-sterile (MS) and restorer (RS) cotton lines expressing BECLIN1 and COP1L105A, respectively. Cytological observation showed that post-meiotic tapetal expression of BECLIN1 delays tapetum developmental programmed cell death (dPCD) by affecting reactive oxygen species (ROS) balance-this delay in dPCD results in early microspore defects and later small-sized flowers with indehiscent anthers. Furthermore, the evaluation of F1 hybrids developed by crossing MS and RS lines showed that early tapetal COP1L105A expression abolishes expression of BECLIN1 resulting in normal tapetum degeneration, pollen development, and fertility. In addition, the F1 hybrid developed with MS and RS cotton lines in transgenic glass-house and net-house conditions showed the rescued fertility comparable with control plants (WT). In terms of cotton fiber productivity, the COP1L105A-expressing transgenic cotton lines outperformed the WT. The current work effectively demonstrates the wider applicability of the new F1 cotton production system.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.1,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SUMO-conjugating enzyme OsSCE1a from wild rice regulates the functional stay-green trait in rice. 野生稻的 SUMO 结合酶 OsSCE1a 调节水稻的功能性留绿性状。

IF 10.1 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-25 DOI: 10.1111/pbi.14524

Xuzhao Yuan, Yanfang Luan, Dong Liu, Jian Wang, Jianxiang Peng, Jinlei Zhao, Lupeng Li, Jingjing Su, Yang Xiao, Yuanjie Li, Xin Ma, Xiaoyang Zhu, Lubin Tan, Fengxia Liu, Hongying Sun, Ping Gu, Ran Xu, Peijiang Zhang, Zuofeng Zhu, Chuanqing Sun, Yongcai Fu, Kun Zhang

{"title":"The SUMO-conjugating enzyme OsSCE1a from wild rice regulates the functional stay-green trait in rice.","authors":"Xuzhao Yuan, Yanfang Luan, Dong Liu, Jian Wang, Jianxiang Peng, Jinlei Zhao, Lupeng Li, Jingjing Su, Yang Xiao, Yuanjie Li, Xin Ma, Xiaoyang Zhu, Lubin Tan, Fengxia Liu, Hongying Sun, Ping Gu, Ran Xu, Peijiang Zhang, Zuofeng Zhu, Chuanqing Sun, Yongcai Fu, Kun Zhang","doi":"10.1111/pbi.14524","DOIUrl":"https://doi.org/10.1111/pbi.14524","url":null,"abstract":"The functional stay-green trait is a major goal of rice breeding. Here, we cloned OsSCE1a encoding SUMO-conjugating enzyme from Yuanjiang common wild rice, which simultaneously regulates the functional stay-green trait and growth duration. Low expression or knocking out OsSCE1a corresponded to increased chlorophyll content, photosynthetic competence, N use efficiency and a shortened growth period without affecting yield. A natural MITE-transposon insertion/deletion in the OsSCE1a promoter is the functional variation that regulates these traits. OsSCE1a was selected during evolution and shows significant variation between indica and japonica rice. OsNAC2 interacts with the MITE to enhance OsSCE1a expression. Genetic manipulation of OsSCE1a revealed its potential for rice improvement. OsSCE1a-mediated SUMOylation of OsGS2 suppresses GS (involved in N assimilation) enzyme activity. OsSCE1a also regulates growth duration by SUMOylating the transcription factor such as OsGBP1, which regulates the expression of the key heading gene Ghd7. Our findings shed light on the role of SUMOylation in crops and provide a strategy for increasing agricultural productivity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.1,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (−)-sparteine 利用狭叶羽扇豆的代谢工程生产对映体纯度高的(-)-天冬氨酸

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-22 DOI: 10.1111/pbi.14509

Davide Mancinotti, Ting Yang, Fernando Geu-Flores

{"title":"Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (−)-sparteine","authors":"Davide Mancinotti, Ting Yang, Fernando Geu-Flores","doi":"10.1111/pbi.14509","DOIUrl":"https://doi.org/10.1111/pbi.14509","url":null,"abstract":"The protein crops known as lupins have been bred to accumulate low levels of antinutritional alkaloids, neglecting their potential as sources of valuable metabolites. Here, we engineered narrow-leafed lupin (NLL) to accumulate large amounts of a single alkaloid of industrial interest called (−)-sparteine. While (−)-sparteine is recognized as a key auxiliary molecule in chiral synthesis, its variable price and limited availability have prevented its large-scale use. We identified two enzymes that initiate the conversion of (−)-sparteine to a variety of alkaloids accumulating in NLL. The first one is a cytochrome P450 monooxygenase belonging to family 71 (CYP71D189), and the second one is a short-chain dehydrogenase/reductase (SDR1). We screened a non-GMO NLL mutant library and isolated a knockout in CYP71D189. The knockout displayed an altered metabolic profile where (−)-sparteine accounted for 96% of the alkaloid content in the seeds (GC–MS basis). The (−)-sparteine isolated from the mutant seeds was enantiomerically pure (99% enantiomeric excess). Apart from the altered alkaloid profile, the mutant did not have any noticeable phenotype. Our work demonstrates that (−)-sparteine is the precursor of most QAs in NLL and expands the current uses of NLL as a crop.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"41 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-produced SARS-CoV-2 antibody engineered towards enhanced potency and in vivo efficacy 植物生产的 SARS-CoV-2 抗体经过工程设计，具有更强的效力和体内疗效

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-19 DOI: 10.1111/pbi.14458

Steven W. de Taeye, Loïc Faye, Bertrand Morel, Angela I. Schriek, Jeffrey C. Umotoy, Meng Yuan, Natalia A. Kuzmina, Hannah L. Turner, Xueyong Zhu, Clemens Grünwald-Gruber, Meliawati Poniman, Judith A. Burger, Tom G. Caniels, Anne-Catherine Fitchette, Réjean Desgagnés, Virginie Stordeur, Lucie Mirande, Guillaume Beauverger, Godelieve de Bree, Gabriel Ozorowski, Andrew B. Ward, Ian A. Wilson, Alexander Bukreyev, Rogier W. Sanders, Louis-Philippe Vezina, Tim Beaumont, Marit J. van Gils, Véronique Gomord

{"title":"Plant-produced SARS-CoV-2 antibody engineered towards enhanced potency and in vivo efficacy","authors":"Steven W. de Taeye, Loïc Faye, Bertrand Morel, Angela I. Schriek, Jeffrey C. Umotoy, Meng Yuan, Natalia A. Kuzmina, Hannah L. Turner, Xueyong Zhu, Clemens Grünwald-Gruber, Meliawati Poniman, Judith A. Burger, Tom G. Caniels, Anne-Catherine Fitchette, Réjean Desgagnés, Virginie Stordeur, Lucie Mirande, Guillaume Beauverger, Godelieve de Bree, Gabriel Ozorowski, Andrew B. Ward, Ian A. Wilson, Alexander Bukreyev, Rogier W. Sanders, Louis-Philippe Vezina, Tim Beaumont, Marit J. van Gils, Véronique Gomord","doi":"10.1111/pbi.14458","DOIUrl":"https://doi.org/10.1111/pbi.14458","url":null,"abstract":"Prevention of severe COVID-19 disease by SARS-CoV-2 in high-risk patients, such as immuno-compromised individuals, can be achieved by administration of antibody prophylaxis, but producing antibodies can be costly. Plant expression platforms allow substantial lower production costs compared to traditional bio-manufacturing platforms depending on mammalian cells in bioreactors. In this study, we describe the expression, production and purification of the originally human COVA2-15 antibody in plants. Our plant-produced mAbs demonstrated comparable neutralizing activity with COVA2-15 produced in mammalian cells. Furthermore, they exhibited similar capacity to prevent SARS-CoV-2 infection in a hamster model. To further enhance these biosimilars, we performed three glyco- and protein engineering techniques. First, to increase antibody half-life, we introduced YTE-mutation in the Fc tail; second, optimization of N-linked glycosylation by the addition of a C-terminal ER-retention motif (HDEL), and finally; production of mAb in plant production lines lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO). These engineered biosimilars exhibited optimized glycosylation, enhanced phagocytosis and NK cell activation capacity compared to conventional plant-produced S15 and M15 biosimilars, in some cases outperforming mammalian cell produced COVA2-15. These engineered antibodies hold great potential for enhancing in vivo efficacy of mAb treatment against COVID-19 and provide a platform for the development of antibodies against other emerging viruses in a cost-effective manner.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142672916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creation of high-resistant starch rice through systematic editing of amylopectin biosynthetic genes in rs4. 通过系统编辑 rs4 中的直链淀粉生物合成基因，创造高抗淀粉水稻。

IF 10.1 1区生物学

Plant Biotechnology Journal Pub Date : 2024-11-19 DOI: 10.1111/pbi.14511

Anqi Wang, Qiao Cheng, Wenjia Li, Mingxi Kan, Yuxin Zhang, Xiangbing Meng, Hongyan Guo, Yanhui Jing, Mingjiang Chen, Guifu Liu, Dianxing Wu, Jiayang Li, Hong Yu

{"title":"Creation of high-resistant starch rice through systematic editing of amylopectin biosynthetic genes in rs4.","authors":"Anqi Wang, Qiao Cheng, Wenjia Li, Mingxi Kan, Yuxin Zhang, Xiangbing Meng, Hongyan Guo, Yanhui Jing, Mingjiang Chen, Guifu Liu, Dianxing Wu, Jiayang Li, Hong Yu","doi":"10.1111/pbi.14511","DOIUrl":"10.1111/pbi.14511","url":null,"abstract":"Resistant starch (RS) is a special kind of starch with beneficial effects on obesity, type 2 diabetes and other chronic complications. Breeding high-RS rice varieties is considered a valuable way to improve public health. However, most rice cultivars only contain an RS level lower than 2% in cooked rice, and cloning of RS genes is critical to improve RS levels in rice. The loss of function of Starch Synthases IIIa (SSIIIa) and SSIIIb, two amylopectin biosynthetic genes, could elevate RS levels up to 10%. Here, we performed a systematic genetic study of 14 amylopectin biosynthetic genes in the ssIIIa ssIIIb double mutant via genome editing, and investigated their effects on RS formation, the eating quality and grain yield. The results showed that deficiency in SSIIa, SSIVb or ISA2 under the ssIIIa ssIIIb background could each elevate RS content to above 14%, and the quadruple mutants of sbeI sbeIIb ssIIIa ssIIIb and sbeI ssIVb ssIIIa ssIIIb could further increase RS levels to over 18%. Furthermore, the eating quality of cooked rice and grain yield decreased along with the elevated RS contents, showing a trade-off among these traits. In these mutants, ssIIIa ssIIIb showed the balanced performance of RS and grain yield. This study provides insights into RS biosynthesis with a series of RS genes in the amylopectin biosynthesis pathway and practical strategy to breed high-RS rice varieties with balanced performance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0