Plant Biotechnology Journal最新文献

{"title":"Two Transcriptional Factors Antagonistically Fine‐Tune MIR1863a to Balance Resistance and Yield Traits in Rice","authors":"Zhang‐Wei Hu, Jun‐Hua Wang, Xiao‐Yu Xiong, Hao Su, Yuan Yang, Yu‐Hui Xiao, De‐Qiang Li, Yong Zhu, Xue‐Mei Yang, Xin‐Ya Chang, He Wang, Guo‐Bang Li, Zhi‐Xue Zhao, Ji‐Wei Zhang, Yan‐Yan Huang, Jing Fan, Wen‐Ming Wang, Yan Li","doi":"10.1111/pbi.70285","DOIUrl":"https://doi.org/10.1111/pbi.70285","url":null,"abstract":"Immune activation usually trades off growth, leading to yield loss. Genes that coordinate resistance and yield are urgently required for crop improvement. MicroRNAs (miRNAs) provide rich candidates for coordinating resistance with yield. Here, we demonstrated that mutation of <jats:italic>MIR1863a</jats:italic> enhances blast disease resistance without yield loss accompanying increased panicle number. However, overexpression and mutation of <jats:italic>MIR1863a</jats:italic> both lead to compromised yield traits such as seed setting rate. The expression of <jats:italic>MIR1863a</jats:italic> was antagonistically regulated by two transcription factors, MADS51 and ESR1. MADS51 activates, whereas ESR1 suppresses <jats:italic>MIR1863a</jats:italic> in leaves and panicles during the growth period and upon pathogen invasion, thus avoiding a penalty in yield caused by excessive resistance. Consistent with the phenotype of <jats:italic>mir1863a</jats:italic> lines, mutation of <jats:italic>MADS51</jats:italic> boosts blast disease resistance without yield loss. Moreover, the haplotypes of <jats:italic>MADS51</jats:italic> and <jats:italic>ESR1</jats:italic>, which exhibit differential activity on the <jats:italic>MIR1863a</jats:italic> promoter, correlate with disease phenotypes in diverse <jats:italic>Japonica</jats:italic> and <jats:italic>Indica</jats:italic> rice accessions, implying a subspecies‐specific evolution of these ‘transcriptional factor‐<jats:italic>MIR1863a</jats:italic>’ models. Taken together, our results revealed a fine‐tuning mechanism controlling an miRNA amounts to balance disease resistance and yield, advancing our understanding of the regulation of the resistance‐yield trade‐off.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin Soil Amendment Triggers Systemic Plant Disease Resistance Through Enhanced Pattern‐Triggered Immunity","authors":"Moffat Makechemu, Yukihisa Goto, Marc W. Schmid, Helen Zbinden, Victoria Widrig, Moritz Kaufmann, Joël F. Pothier, Beat Keller, Cyril Zipfel","doi":"10.1111/pbi.70282","DOIUrl":"https://doi.org/10.1111/pbi.70282","url":null,"abstract":"Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin‐induced systemic effects in plants remain unknown. In this study, we investigated the effects of soil amendment with crab chitin flakes (hereafter chitin) on pattern‐triggered immunity (PTI) and systemic disease resistance in various plant species. We found that soil amendment with chitin potentiates PTI and disease resistance against the bacterial pathogen <jats:styled-content style=\"fixed-case\"><jats:italic>Pseudomonas syringae</jats:italic></jats:styled-content> pv. tomato DC3000 in lettuce, tomato and Arabidopsis as well as against the fungal pathogen <jats:italic>Blumeria graminis f.</jats:italic> sp. <jats:italic>tritici (Bgt)</jats:italic>, which causes powdery mildew in wheat. Using micrografting in Arabidopsis, we demonstrated that this systemic effect is dependent on active chitin perception in the roots. We also showed that induced systemic resistance (ISR) and pattern‐recognition receptors (PRRs)/coreceptors, but not systemic acquired resistance (SAR), are involved in the systemic effects triggered by chitin soil amendment. These systemic effects correlated with the transcriptional upregulation of key PTI regulators in distal leaves upon chitin soil amendment. Notably, chitin‐triggered systemic immunity was independent of microbes present in soil or chitin flakes. Together, these findings contribute to a better understanding of chitin‐triggered systemic immunity, from active chitin perception in roots to the potentiation of PTI in the leaves, ultimately priming plants to mount enhanced defence responses against pathogen attacks. Our study provides valuable insights into the molecular mechanisms of chitin soil amendment and resulting induced immunity and highlights its potential use for sustainable crop protection strategies.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"712 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel ZjbZIP33‐ZjPRX1 Module Positively Regulates Lignin Formation in the Jujube Fruit Stone","authors":"Yao Zhang, Shijia Li, Yin Liu, Huibin Wang, Li Dai, Yuehan Qi, Jingyi Zhou, Zhihui Zhao, Ping Liu, Lixin Wang, Jin Zhao","doi":"10.1111/pbi.70280","DOIUrl":"https://doi.org/10.1111/pbi.70280","url":null,"abstract":"Fruit stones play an important role in fruit development and are closely related to fruit quality. Lignification of the fruit stone (endocarp) is a typical feature of stone fruits. Stoneless jujube is a valuable jujube germplasm resource, and the degree of lignification of its fruit stone is low. However, the underlying mechanisms of lignification in the jujube endocarp remain unclear. In this study, we characterised a novel Class III peroxidase, PRX1, via comparative transcriptome analysis of the jujube endocarp and investigated its potential molecular mechanism in endocarp lignin synthesis. Subcellular localisation analysis revealed that ZjPRX1 was localised to the cytoplasm. According to the results of an in situ hybridisation analysis, the tissue‐specific expression of <jats:italic>ZjPRX1</jats:italic> was greatest in the endocarp. Moreover, <jats:italic>ZjPRX1</jats:italic> overexpression in jujube fruits and calluses caused significant changes in the amount of lignin that accumulated. Scanning electron microscopy revealed that the cell walls of the stems of <jats:italic>ZjPRX1</jats:italic>‐OE <jats:italic>Arabidopsis</jats:italic> lines were significantly thicker than those of WT <jats:italic>Arabidopsis</jats:italic>. Yeast one‐hybrid, dual‐luciferase reporter and electrophoretic mobility shift assays indicated that ZjbZIP33 activated <jats:italic>ZjPRX1</jats:italic> expression by binding specifically to the G‐box element in the <jats:italic>ZjPRX1</jats:italic> promoter. IAA and GA<jats:sub>3</jats:sub> induced ZjbZIP33‐<jats:italic>ZjPRX1</jats:italic> expression and lignin formation. Overall, the results revealed a novel module, that is, ZjbZIP33‐<jats:italic>ZjPRX1</jats:italic>, that positively regulates lignin formation in the jujube fruit stone. This study contributes to a better understanding of the molecular basis of lignin synthesis in the endocarp of jujube fruits and identifies candidate genes for optimising stone fruit breeding.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"13 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advance in the assembly of the plant mitochondrial genomes using high‐throughput DNA sequencing data of total cellular DNAs","authors":"Yang Ni, Jingling Li, Yihui Tan, Guoan Shen, Chang Liu","doi":"10.1111/pbi.70249","DOIUrl":"https://doi.org/10.1111/pbi.70249","url":null,"abstract":"SummaryThe assembly of plant mitochondrial genomes presents unique challenges due to difficulties in isolating mitochondrial DNA (mtDNA) and plant mitochondrial genome characteristics, such as low interspecific conservation; sequence sharing among mitochondrial, nuclear and plastid DNAs; and complex structural variations. Our laboratory has sequenced and assembled a dozen plant mitochondrial genomes, testing various strategies and identifying numerous assembly issues. This review compared the advanced methods and tools for plant mitochondrial genome assembly, categorizing assembly algorithms into three groups: (1) reference‐based, (2) de novo and (3) iterative mapping and extension. The performance of 11 software tools used most frequently over the past 5 years (GetOrganelle, Velvet, NOVOPlasty, SOAPdenovo2, Canu, Flye, SMARTdenovo, PMAT, NextDenovo, SPAdes and Unicycler) and two newly developed tools (TIPPo and Oatk) was assessed. The evaluation metrics included the completeness, contiguity and correctness of the assembled plant mitochondrial genomes. SMARTdenovo, NextDenovo and Oatk demonstrated superior performance in terms of contiguity and completeness. GetOrganelle and Flye excelled in correctness. Key challenges in plant mitochondrial genome assembly, such as removing nuclear mitochondrial DNA (NUMT) and mitochondrial plastid DNA (NUPT) contamination and resolving intra‐genomic repetitive regions, were discussed. A general strategy for plant mitochondrial genome assembly used in studies conducted in our laboratory was summarized. This review serves as a resource for those assembling plant mitochondrial genomes or developing plant mitochondrial genome assembly tools.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of StHsfA2 Enhances Thermotolerance and Promotes Tuberisation in Potato Under High Temperature Through StSP6A.","authors":"Runlong Zhang,Wenbin Du,Qian He,Danqing Li,Bote Luo,Lingyan Cao,Xiuyun Wang,Zixian Zeng,Jian Wu,Cheng Chen","doi":"10.1111/pbi.70283","DOIUrl":"https://doi.org/10.1111/pbi.70283","url":null,"abstract":"High temperature (HT) is a major environmental stress that severely inhibits potato (Solanum tuberosum L.) tuberisation and yield. Heat shock transcription factors (Hsfs) are pivotal in plant thermotolerance, yet their roles in potato remain unclear. Here, we demonstrate that overexpression of StHsfA2, a rapidly HT-responsive HSF family member, enhances thermotolerance and mitigates yield loss in transgenic potato under HT conditions. We reveal that StHsfA2 upregulates StSP6A expression by binding to the heat shock element-like motifs in its promoter. StSP6A encodes a homologue of FLOWERING LOCUS T that is critical for initiating tuber formation. Intriguingly, we found that StHsfA2 physically interacts with the StSP6A protein, which in turn inhibits StHsfA2-mediated StSP6A upregulation. However, HT stress attenuates the StHsfA2-StSP6A interaction. Thus, a negative feedback loop modulates StSP6A regulation by StHsfA2 under HT. In summary, our study shows that StHsfA2 is a key regulator of thermotolerance in potato plants. Its overexpression enhances heat resistance and could boost tuber yield, making it a promising candidate gene for countering yield loss amid global warming.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward Translation of Cowpea Mosaic Virus Intratumoral Immunotherapy With a Scalable Production Process","authors":"Patrick Opdensteinen, Jessica Fernanda Affonso de Oliveira, Seongwon Jung, Nicole F. Steinmetz","doi":"10.1111/pbi.70281","DOIUrl":"https://doi.org/10.1111/pbi.70281","url":null,"abstract":"The plant virus cowpea mosaic virus (CPMV) is being developed as a drug candidate for intratumoral immunotherapy for solid tumours. Data from preclinical studies in mice and rats as well as clinical studies in canine cancer patients demonstrate potent efficacy and an excellent safety profile. Toward Investigational New Drug (IND)‐enabling studies, a scalable and Good Manufacturing Practice (GMP)‐compatible manufacturing process must be developed. Therefore, here, we focused on the downstream process and developed a scalable purification process. We systematically screened the following processes: acidic extraction conditions making use of the virion pH stability, ultrafiltration making use of the nanoparticle character and the virion size, and finally ion exchange chromatography based on the virion's surface charge. The three processes were then combined into a 7‐step protocol, providing efficiency compared to the contemporary laboratory procedures involving various centrifugation steps (and a total of 15 steps to yield pure CPMV). Furthermore, we streamlined the removal of endotoxins by combining detergent‐based endotoxin removal with ion exchange chromatography. The new ultrafiltration‐based process reduced the number of unit operations by more than half and the processing time from ~20 to ~7 h compared to centrifugation‐based purification of CPMV. Importantly, toxic organic solvents, ultracentrifugation and isopycnic ultracentrifugation which are difficult to scale were omitted. We used a battery of characterisation methods to validate CPMV's structural integrity and biological activity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"41 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PagSAMDC4a‐Mediated Polyamine Synthesis Regulate Vessel Differentiation Under Drought Stress Conditions in Poplar","authors":"Junguang Yao, Miaoxia Li, Zirui Wu, Cheng Jiang, Yi An, Lichao Huang, Ningning Chen, Jin Zhang, Mengzhu Lu","doi":"10.1111/pbi.70284","DOIUrl":"https://doi.org/10.1111/pbi.70284","url":null,"abstract":"Frequent and prolonged drought stress is a primary factor limiting the growth and development of perennial woody plants. Xylem vessel cells are crucial for water transport in woody plants; however, the molecular mechanisms governing the changes in their morphogenesis in response to water deprivation remain unclear. Here, we report that the alterations in polyamine (PA) levels under drought stress, mediated by the key enzyme PagSAMDC4a in PA synthesis, influence vessel morphogenesis through the modulation of hydrogen peroxide (H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>) concentration in poplar 84 K (<jats:styled-content style=\"fixed-case\"><jats:italic>Populus alba</jats:italic></jats:styled-content> × <jats:italic>Populus glandulosa</jats:italic>). <jats:italic>PagSAMDC4a</jats:italic> expression was predominantly observed in the phloem and cambium zones of stems and was upregulated under drought conditions. Overexpression of <jats:italic>PagSAMDC4a</jats:italic> (<jats:italic>PagSAMDC4a</jats:italic>‐OE) resulted in increased PA content, leading to reduced vessel size and density in stem xylem. Conversely, <jats:italic>PagSAMDC4a</jats:italic> mutant lines exhibited the opposite phenotype, resembling 84 K plants treated with the PA synthesis inhibitor methylglyoxal‐bis (guanylhydrazone). In addition, <jats:italic>PagSAMDC4a</jats:italic>‐OE exhibited improved drought tolerance compared to nontransgenic 84 K plants and <jats:italic>PagSAMDC4a</jats:italic> mutant lines. Interestingly, the H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> level was significantly decreased in <jats:italic>PagSAMDC4a</jats:italic>‐OE plants but markedly increased in <jats:italic>PagSAMDC4a</jats:italic> mutants relative to 84 K plants, suggesting that changes in vessel size may result from altered H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> levels. Supporting this hypothesis, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> application rescued the small vessel phenotype in <jats:italic>PagSAMDC4a</jats:italic>‐OE, whereas the scavenger potassium iodide reduced the size of xylem vessels in <jats:italic>PagSAMDC4a</jats:italic> mutants. Collectively, these findings provide evidence for the role of PAs in enhancing drought tolerance through the regulation of vessel differentiation.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"125 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.)","authors":"Jorge Lagrèze, Antonio Santiago Pajuelo, Daniele Coculo, Bárbara Rojas, Gaston A. Pizzio, Chen Zhang, Meng‐Bo Tian, Mickael Malnoy, Alessandro Vannozzi, Lorenza Dalla Costa, Vincenzo Lionetti, José Tomás Matus, Giulia Malacarne","doi":"10.1111/pbi.70279","DOIUrl":"https://doi.org/10.1111/pbi.70279","url":null,"abstract":"<jats:italic>Botrytis cinerea</jats:italic> (Bc) is a major pathogen of cultivated grapevine (<jats:styled-content style=\"fixed-case\"><jats:italic>Vitis vinifera</jats:italic></jats:styled-content> L.), with cell wall (CW) remodelling playing a critical role in fungal colonisation. CW‐modifying enzymes, particularly pectin methylesterases (PMEs), produced by both host and pathogen, influence CW integrity and the outcome of infection. To explore the role of CW composition and remodelling in grapevine's response to Bc, we inoculated three genotypes with varying susceptibility at full flowering. Biochemical analysis of flowers and ripe berry skins revealed that the tolerant genotype exhibited significantly higher PME activity postinfection compared with the susceptible ones. Unbiased transcriptome analysis of infected flower tissues showed a more intense transcriptional response in the susceptible genotype, suggesting an ultimately ineffective attempt to restrict fungus spread. Expression profiling of 62 <jats:italic>PME</jats:italic> genes in this data set and public Bc‐infected berry transcriptomes identified <jats:italic>PME10</jats:italic> as the most strongly induced gene upon infection. <jats:italic>PME10</jats:italic> knockout mutants displayed reduced PME activity and heightened susceptibility, while overexpression lines showed enhanced PME activity and reduced disease symptoms. Gene co‐expression network analysis highlighted WRKY03, a defence‐related transcription factor, as a putative regulator of <jats:italic>PME10</jats:italic>. DAP‐seq, DAP‐qPCR and dual luciferase assays confirmed direct binding and activation of the <jats:italic>PME10</jats:italic> promoter by WRKY03. Altogether, this study demonstrates that PME10 is a functional PME contributing to grapevine immunity against <jats:styled-content style=\"fixed-case\"><jats:italic>B. cinerea</jats:italic></jats:styled-content>, establishing it as a key component of the grapevine defence machinery against fungal pathogens.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.).","authors":"Shuhe Zhang,Lin Zhang,Huicai Cai,Jiahao Zhang,Zhenning Yang,Qiang Yan,Fuju Wu,Yingzhe Wang,Bo Xu","doi":"10.1111/pbi.70276","DOIUrl":"https://doi.org/10.1111/pbi.70276","url":null,"abstract":"Anthers consist of various specialised cell types and play a significant role in plant reproduction. Although the molecular mechanisms underlying anther development and regulation have been extensively studied, the single-cell transcriptional landscape and dynamic regulation during anther development in M. sativa remain largely unexplored. In the present study, we constructed the first single-cell transcriptome atlas of M. sativa anthers to provide a comprehensive view of cell type-specific gene expression and epigenetic modifications. The reconstruction of the developmental trajectories of tapetum and microspores led to the identification of novel genes and elucidated the regulatory networks involved in tapetum formation. Our findings revealed rapid functional transitions in tapetum during the tetrad stage, including cell formation, specialisation, and programmed cell death (PCD). Additionally, we analysed the stages at which tapetal degradation and microspore shrinkage occurred in the sterile line. Overall, this study offers valuable insights into the molecular mechanisms underlying anther development in M. sativa. Specifically, MsKIN14P was identified as a key regulator of microtubules and the cytoskeleton during mitosis, and the transcription factors ERF3 and ERF025 were shown to influence anther development through the ethylene response pathways. These findings provide an essential theoretical foundation for the development of novel male-sterile lines and enhance the breeding capabilities of M. sativa.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression of Arabidopsis FLS2 in Nicotiana benthamiana confers responsiveness to Ralstonia flg22 and activation of anti-bacterial immunity.","authors":"Meng Li, Yujiao Chen, Yali Wei, Alberto P Macho","doi":"10.1111/pbi.70255","DOIUrl":"https://doi.org/10.1111/pbi.70255","url":null,"abstract":"<p><p>The association between Arabidopsis FLS2 and BAK1/SERK3 triggered by bacterial flagellin is the best-studied example of plant PRR receptor/co-receptor complex. Although most plants are able to perceive the flagellin epitope flg22 from most bacterial species, certain pathogens, such as Agrobacterium tumefaciens or Ralstonia solanacearum, have evolved polymorphisms in their flg22 sequence, avoiding perception by most plants tested so far. Specific plant species, such as Vitis riparia or Glycine max, respectively, have subsequently evolved FLS2 versions with the ability to recognize these polymorphic flg22 peptides. The model plants Arabidopsis or N. benthamiana are not able to perceive the flg22 peptide from R. solanacearum (flg22^Rso). However, in this work, we found that the expression of AtFLS2 in N. benthamiana confers responsiveness to flg22^Rso, leading to the activation of specific downstream responses. AtFLS2 interacts with the BAK1 orthologs in N. benthamiana (NbSERKs), which contribute to flg22^Rso recognition by AtFLS2 and subsequent immune responses. Finally, we found that this recognition activates anti-bacterial immunity that restricts Ralstonia growth, suggesting that cross-species PRR-co-receptor compatibility could be exploited for the development of tailored immunity against pathogens with polymorphic PAMPs.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}