Plant Biotechnology Journal最新文献

{"title":"Engineering Herbicide Cross-Resistance in Rapeseed by Generating Stacked BnaALS Mutations via Sequential CBE and ABE8e-SpRY Editing.","authors":"Sichao Ren, Wenjing Lei, Jiaqi He, Meng Jia, Yu Liu, Jialin Fan, Yumei Wang, Li Lin, Hui Zhang, Youping Wang, Jian Wu","doi":"10.1111/pbi.70670","DOIUrl":"https://doi.org/10.1111/pbi.70670","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsMYB306-OsRAV11 Regulates Resistance of Rice to Striped Stem Borer by Modulating Serotonin Biosynthesis.","authors":"Jia-Run Zhang, Long Wang, Yang-Fan Chen, Xiao-Hao Guo, Meng Jiang, Yuan-Yuan Tan, Qing Wang, Qiu Qian, Angharad M R Gatehouse, Qing-Yao Shu","doi":"10.1111/pbi.70680","DOIUrl":"https://doi.org/10.1111/pbi.70680","url":null,"abstract":"<p><p>Striped stem borer (SSB; Chilo suppressalis Walker) is one of the most destructive pests in rice production. Previous studies have demonstrated that SSB infestation induces transcription of OsT5H (tryptamine-5-hydroxylase) and biosynthesis of serotonin, a newly recognised phytohormone, and that disruption of serotonin biosynthesis significantly increases SSB resistance. However, the regulatory module modulating serotonin biosynthesis remains to be identified and characterised. Here, we reveal an OsMYB306-OsRAV11 module that regulates OsT5H transcription and serotonin biosynthesis in response to SSB infestation in rice. OsMYB306 and OsRAV11 can bind to the OsT5H promoter and repress its transcription. In the module, OsRAV11 interacts with OsMYB306 and enhances its inhibitory effect on OsT5H transcription. CRISPR/Cas9-generated knockout mutants (myb306, rav11 and myb306 rav11) exhibited elevated OsT5H expression, increased serotonin accumulation and reduced SSB resistance. Conversely, OsRAV11 overexpression reduced OsT5H transcription. Our findings establish a transcriptional regulatory framework for the biosynthesis of serotonin in response to SSB infestation. These findings inform the development of new strategies for producing SSB-resistant rice by genome editing, potentially reducing reliance on chemical pesticides for SSB control.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of Prime Editors for the Induction of Site-Specific, Heritable Edits in Soybean [Glycine max (L.) Merr.].","authors":"Nirmal Khadka, Hien Thuy Bui, Ajay Gupta, Steve A Whitham, Bing Yang","doi":"10.1111/pbi.70678","DOIUrl":"https://doi.org/10.1111/pbi.70678","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Modified Cas9 Scaffold Allows Extension of the Virus-Induced Gene Editing Technology to the Large Potyvirus Genus.","authors":"Fernando Merwaiss, Verónica Aragonés, Arcadio García, José-Antonio Daròs","doi":"10.1111/pbi.70675","DOIUrl":"https://doi.org/10.1111/pbi.70675","url":null,"abstract":"<p><p>Plant viruses are recognized as rapid and effective vectors to deliver CRISPR-Cas reaction components into plants, a strategy termed virus-induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery of the editing components to new cultivars. Potyviruses (genus Potyvirus) comprise the largest group of plant RNA viruses. The main limitation of potyviral vectors to express a non-coding RNA consists of potential insertion of stop codons that interrupt the large open reading frame that encompasses most potyviral genome. This is the case with the Streptococcus pyogenes Cas9 sgRNA scaffold, which contains stop codons in all three possible frames. In this work, we first built on a visual reporter system targeting the two homeologs of Nicotiana benthamiana Magnesium chelatase subunit I (CHLI). Second, we developed a tobacco etch virus (Potyvirus nicotianainsculpentis)-derived vector for VIGE by engineering a modified Cas9 scaffold, free of stop codons, to maintain the potyviral polyprotein reading frame while ensuring effective editing. This vector self-replicates and moves systemically, delivering sgRNAs efficiently throughout the plant. This allowed us to obtain plants exhibiting a white phenotype with their four alleles edited through in vitro regeneration from infected leaves, and also to produce edited progeny. We further demonstrated the vector utility in tomato. Given the conserved biological properties within the genus Potyvirus, these findings may be broadly applicable to other potyviruses, expanding the reach of the VIGE technology.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High Soluble-Fibre Allele in Wheat Encodes a Defective Cell Wall Peroxidase Responsible for Dimerization of Ferulate Moieties on Arabinoxylan.","authors":"Rowan A C Mitchell, Ondrej Kosik, Abdul Kader Alabdullah, Anneke Prins, Maria Oszvald, Till K Pellny, Jackie Freeman, Kirstie Halsey, Caroline A Sparks, Alison Huttly, James Brett, Michelle Leverington-Waite, Simon Griffiths, Peter R Shewry, Alison Lovegrove","doi":"10.1111/pbi.70527","DOIUrl":"10.1111/pbi.70527","url":null,"abstract":"<p><p>Increasing dietary fibre (DF) intake is an important target to improve health. An attractive strategy for this is to increase DF in wheat which is derived principally from the endosperm cell wall polysaccharide arabinoxylan (AX). The water-extractable form of this (WE-AX) accounts for most soluble dietary fibre (SDF), which is believed to confer particular health benefits. A region of chromosome 6B in some wheat varieties confers high SDF and here we show that the cause is an allele encoding a peroxidase family protein with a single residue change (PER1-v) associated with high WE-AX, compared to the more common form (PER1). Both wheat lines carrying this natural PER1-v variant and those with an induced knockout mutation of PER1 showed reduced dimerization of endosperm ferulate consistent with a mechanism of decreased cross-linking in the cell wall that increases WE-AX. Transiently expressed PER1_RFP fusion protein driven by the native promoter in wheat endosperm was shown to localise to cell walls, whereas PER1-v_RFP did not. We therefore propose that PER1-v lacks the capacity to dimerise AX ferulate in vivo due to mis-localisation caused by the missense single-nucleotide polymorphism (SNP) in the PER1-v allele, so that the SNP acts as a perfect marker. This marker can be used to identify current wheat varieties with high WE-AX to be used by processors and by breeders to ensure future varieties have high WE-AX to make healthier wheat-based foods.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"2751-2761"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145891894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Super-Pangenome for Cultivated Citrus Reveals Evolutive Features During the Allopatric Phase of Their Reticulate Evolution.","authors":"Gaetan Droc, Delphine Giraud, Caroline Belser, Karine Labadie, Simone Duprat, Corinne Cruaud, Benjamin Istace, Fredson Dos Santos Menezes, Edson Mario de Andrade Silva, Franck Curk, Gilles Costantino, Alexandre Soriano, Pierre Mournet, Alexis Dereeper, Maëva Miranda, Elodie Marchi, Sylvain Santoni, Raner José Santana Silva, Stéphanie Sidibe-Bocs, François Luro, Nathalie Choisne, Florian Maumus, Barbara Hufnagel, Fabienne Micheli, Patrick Wincker, Jean-Marc Aury, Arnaud Lemainque, Patrick Ollitrault","doi":"10.1111/pbi.70553","DOIUrl":"10.1111/pbi.70553","url":null,"abstract":"<p><p>The main genetic diversity observed in cultivated citrus results from a reticulate evolution involving four ancestral taxa whose radiation occurred in allopatry. In such context, GWAS analysis, genome diversity and transcriptomic studies will be significantly enhanced through pangenome approaches. We report the implementation of a super-pangenome for cultivated citrus, established with de novo assemblies of C. medica, C. reticulata and C. micrantha, released for the first time alongside a published chromosome-scale assembly of C. maxima. Repetitive element annotation revealed that half of each genome consisted of transposable elements or DNA-satellites. The new genome assemblies display strong synteny and collinearity, while discrepancies are observed with the C. maxima assembly. Resequencing information from 55 accessions helped to explore the intra- and interspecific diversity of the ancestral taxa and their relationships with horticultural groups. Diagnostic SNPs of the ancestral taxa revealed interspecific introgressions in several representative accessions of C. reticulata, C. maxima and C. medica as well as insights into the origin and phylogenomic structures of horticultural groups. PAV analysis revealed a gene whose absence or presence was specific to one of the ancestral taxa. Diagnostic PAV analysis uncovered a large chloroplastic introgression in C. medica chromosome 4. The analysis of the functional enrichment and species-specific adaptations in the citrus super-pangenome revealed distinct functional specialisations. This highlights the evolutionary paths that have shaped species, contributing to the diversity in the citrus super-pangenome while maintaining a shared foundation of essential biological processes. We established a Genome Hub, offering a platform for continuous genomic research.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"3345-3367"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress Granule-Associated ZmCTU2 Confers Thermotolerance in Maize via Coordinated Regulation of Proteostasis and ROS Homeostasis.","authors":"Yufang Xu, Yudong Fan, Yulian Li, Yilin Zhang, Enpeng Liu, Ruiqi Li, Peipei Ma, Tao Li, Huiyong Zhang","doi":"10.1111/pbi.70568","DOIUrl":"10.1111/pbi.70568","url":null,"abstract":"<p><p>The escalating global temperatures and intensifying heat stress events pose significant threats to maize productivity worldwide. Uncovering key thermotolerance genes and their functional mechanisms is thus critical for developing climate-resilient crops. Here, we report that ZmCTU2, a cytoplasmic tRNA thiolation factor, acts as a central regulator of heat tolerance in maize. Expression of ZmCTU2 correlates positively with kernel-setting under high temperatures. Overexpression of ZmCTU2 confers enhanced thermotolerance at both seedling and adult stages, improving survival and field yield under heat stress, whereas loss-of-function mutants of ZmCTU2 or its partner ZmCTU1 display severe seed developmental defects and lethality. Mechanistically, ZmCTU2 translocates to stress granules under thermal stress, where it recruits ZmCTU1 and ROS-scavenging peroxidases, shielding them from degradation. This dual recruitment facilitates synergistic protective responses: maintenance of tRNA thiolation to ensure translational fidelity, and stabilisation of antioxidative enzymes to bolster redox homeostasis. Our study identifies ZmCTU2 as a scaffold protein within stress granules that coordinates proteostatic and antioxidative pathways under heat stress, providing a valuable genetic resource for engineering thermotolerant maize.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"3371-3390"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational Design of Hyoscyamine 6β-Hydroxylase for the Efficient Production of Scopolamine.","authors":"Yong Meng, Yicheng Wang, Wentang Chen, Junnan Li, Siyuan Liao, Chunxian Yang, Lingjiang Zeng, Xiaozhong Lan, Min Chen, Zhihua Liao, Junlan Zeng","doi":"10.1111/pbi.70562","DOIUrl":"10.1111/pbi.70562","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"3368-3370"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pro-Vitamin A Biofortified Cavendish Banana: Trait Stability in the Field.","authors":"Jimmy M Tindamanyire, Jacinta L Watkins, Cara Mortimer, Bulukani Mlalazi, Jeff Daniells, Rob Harding, James L Dale, Jean-Yves Paul","doi":"10.1111/pbi.70516","DOIUrl":"10.1111/pbi.70516","url":null,"abstract":"<p><p>Vitamin A deficiency (VAD), a major global health concern, has driven efforts to develop staple crops with enhanced pro-vitamin A (pVA) content. Delivering meaningful nutritional benefits, however, requires technologies that maintain elevated carotenoid levels under field conditions. Previous proof-of-concept work demonstrated that pVA content can be substantially increased in Cavendish bananas through genetic modification, providing a platform for transferring the technology into East African Highland banana (EAHB) cultivars relevant to reducing VAD in Uganda. To evaluate performance under agronomic conditions, we conducted multi-generational field assessments of 27 transgenic Cavendish lines generated from seven constructs enabling constitutive or fruit-preferred expression of three carotenoid biosynthesis genes: ZmPsy1, MtPsy2a and PaCrtI. Constitutive expression was driven by the maize Ubi promoter, while fruit expression was regulated by Exp1 or ACO promoters. Agronomic performance and fruit carotenoid levels were analysed across three generations to explore factors influencing pVA enhancement. All transgenic lines exhibited increased fruit pVA, with the highest accumulation observed in lines constitutively expressing MtPsy2a. Promoter-transgene combinations significantly affected carotenoid accumulation and the stability of the trait in the field. PVA accumulation was the highest in the initial sucker crop and declined in subsequent ratoons, reflecting sensitivity to seasonal conditions. While ACO- and Ubi-driven lines were less affected by seasonal temperature changes, these variations significantly constrained pVA accumulation in wild-type and Exp1-regulated lines. This comprehensive assessment helps elucidate the complex interplay of promoter, isoform, and environmental factors that are essential for the long-term viability of nutritional interventions aimed at combating VAD in the region.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"3327-3344"},"PeriodicalIF":10.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ectopic Expression of ScALDH21 From a Desert Moss Enhances Cotton Resistance to Verticillium Wilt via the Modulation of Jasmonates and Phenylpropanoid Pathways","authors":"Honglan Yang, Xiaoshuang Li, Tohir A. Bozorov, Jianwei Zhang, Aerguli Jiamahate, Dawei Zhang, Jiancheng Wang, Qilin Yang, Zongrang Liu, Dina Mahesati, Haijiang Xu, Shuangxia Jin, Daoyuan Zhang, Yongqiang Dai","doi":"10.1111/pbi.70669","DOIUrl":"https://doi.org/10.1111/pbi.70669","url":null,"abstract":"Biotic stresses, particularly Verticillium wilt (VW), lead to a global decline in cotton yields. Here, we demonstrate that ectopic expression of <jats:italic>ScALDH21</jats:italic> , a gene from the desiccation‐tolerant moss <jats:styled-content style=\"fixed-case\"> <jats:italic>Syntrichia caninervis</jats:italic> </jats:styled-content> Mitt. and absent in angiosperms, enhances cotton's resistance to VW. Multi‐year, multiple location field evaluations showed that transgenic cotton lines consistently exhibited two major advantages: markedly improved resistance to VW, and significantly reduced yield loss, with an approximate 23.8% yield increase relative to non‐transgenic counterparts under pathogen infection conditions. This disease resistance is associated with enhanced capacity of the transgenic lines to scavenge reactive oxygen species (ROS), induced by pathogen infection. This finding aligns with the <jats:italic>ScALDH21</jats:italic> ‐conferred detoxification function. Transcriptome analyses revealed a significant alteration in expression pattern of those genes that regulate phenylpropanoid and jasmonic acid (JA) pathways. Correspondingly, the accumulation of lignin and defence‐related metabolites (e.g., rutin, cyanidin and jasmonates) significantly increased, suggesting that <jats:italic>ScALDH21</jats:italic> ‐mediated activation of the phenylpropanoid and JA pathways contributes to enhanced resistance. Analyses of ScALDH21 binding activity using CUT&amp;Tag and EMSA assays showed that it can bind to specific gene promoters within the cotton genome, highlighting that ScALDH21 not only catalyses the detoxification of aldehydes but also gains transcriptional regulatory roles. In summary, we demonstrate that expression of the heterologous <jats:italic>ScALDH21</jats:italic> in cotton leads to enhancement of resistance to VW and elucidated the mechanism. Our findings further demonstrate a promising strategy to improve biotic resistance in crops by utilizing unique functional genes from evolutionarily distant species in extreme environments.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"8 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}