Plant Biotechnology Journal最新文献

{"title":"Mechanistic Insights Into Functional Innovations of Dammarenediol-II Synthase in Panax ginseng.","authors":"Lei Feng,Yan Zhao,Dongju Fu,Xia Li,Bing Hao,Yucheng Zhao,Xiangyu Liu,Guisheng Xiang,Zihan Yang,Fengling Tan,Meiyu Duan,Hanyu Fu,Bolin Wu,Simei He,Yina Wang,Geng Chen,Shuangyan Zhang,Chuyi Wang,Wanling Song,Yuanhong Fan,Guanghui Zhang,Shengchao Yang","doi":"10.1111/pbi.70299","DOIUrl":"https://doi.org/10.1111/pbi.70299","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Rhus chinensis Genome Provides Insights Into Tannin, Flavonoid Biosynthesis, and Glandular Trichome Development","authors":"Zhaogeng Lu, Helin Zou, Jiawen Cui, Tongfei Wang, Lingyu Ma, Shixiong Ren, Yiwei Cao, Xi Zhang, Zixi Chen, Hongyan Bao, Ling Zhu, Yaning Cui, Ruili Li, Xiuyan Yang, Qikun Zhang, Zhili Wang, Wangxiang Zhang, Li Wang, Jinxing Lin, Biao Jin","doi":"10.1111/pbi.70392","DOIUrl":"https://doi.org/10.1111/pbi.70392","url":null,"abstract":"The “salt tree”, <jats:styled-content style=\"fixed-case\"><jats:italic>Rhus chinensis</jats:italic></jats:styled-content>, holds significant economic and medicinal value due to its ability to produce <jats:italic>Galla chinensis</jats:italic> (Chinese gall/gallnut), a plant‐derived medicinal material used in both traditional Chinese and modern medicine that is rich in tannins and flavonoids. It is also renowned for its remarkable stress tolerance. However, the genetic basis underlying its tannin and flavonoid biosynthesis and stress adaptation remains largely unexplored. Here, we assembled a chromosome‐level genome of <jats:styled-content style=\"fixed-case\"><jats:italic>R. chinensis</jats:italic></jats:styled-content> with a size of 357.62 Mb. A significant expansion of defence‐related genes, particularly those involved in chitin catabolism and flavonoid biosynthesis, explains the tree's extensive environmental adaptability. We identified key genes involved in tannin biosynthesis and hydrolysis, with <jats:italic>RcTA1</jats:italic> playing a central role in gallic acid accumulation, a precursor of hydrolyzable tannins. Notably, RcDIV1 promotes tannin hydrolysis by directly activating <jats:italic>RcTA1</jats:italic> transcription. Additionally, we uncovered that well‐developed multicellular glandular trichomes, regulated by RcGL2, along with an expanded array of transporters (e.g., ABCGs) and an enhanced ABA response, play critical roles in mediating salt tolerance. These factors collectively drive the production of salt‐like secretions, including phenolic and organic acids, which coat the fruit surface. Our study provides profound insights into the genetic mechanisms governing abundant tannin accumulation, flavonoid biosynthesis, glandular trichome development, and stress resilience, offering valuable genetic resources for improving the medicinal and ecological traits of this species.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"36 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ZmFKF1b‐ZmDi19‐5 Regulatory Module Coordinates Drought Tolerance and Flowering Time in Maize","authors":"Haixia Zeng, Dandan Dou, Yang Yang, Yan Yan, Yawen Sun, Shuhan Yang, Wen Yao, Shifang Zhao, Mingle Wang, Zhixue Liu, Zhenzhen Ren, Huihui Su, Liru Cao, Lixia Ku, Xu Zheng, Chengwei Li, Yanhui Chen","doi":"10.1111/pbi.70404","DOIUrl":"https://doi.org/10.1111/pbi.70404","url":null,"abstract":"Drought is a major environmental stress that inhibits plant growth and reduces crop yields. The <jats:italic>Di19</jats:italic> gene family is known to play a pivotal role in mediating plant responses to drought. However, the mechanisms by which Di19 proteins integrate drought response with developmental processes, particularly flowering time, remain largely unknown in maize. In this study, we reveal that <jats:italic>ZmDi19‐5</jats:italic> possesses a dual function in regulating both drought tolerance and flowering in maize. Overexpression of <jats:italic>ZmDi19‐5</jats:italic> not only enhanced drought tolerance but also delayed flowering time. Furthermore, we demonstrate that the ZmDi19‐5 and ZmFKF1b proteins interact both in vivo and in vitro. In contrast to the <jats:italic>zmfkf1b</jats:italic> mutants, plants overexpressing <jats:italic>ZmFKF1b</jats:italic> exhibited increased sensitivity to drought and accelerated flowering. Mechanistically, the interaction with ZmFKF1b attenuates the binding of ZmDi19‐5 to the promoter of its downstream targets, including the transcription factor <jats:italic>ZmHsf08</jats:italic> and the flowering inhibitor <jats:italic>ZmCOL3</jats:italic>, subsequently affecting their expression. In conclusion, our findings reveal that the ZmFKF1b‐ZmDi19‐5 module coordinates drought stress responses and flowering time in maize, providing a promising target for breeding drought‐resistant maize varieties with stable agronomic traits.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"25 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TaSPX3 Enhances Wheat Resistance to Leaf Rust by Antagonising TaDi19‐Mediated Repression of Pathogenesis‐Related Genes","authors":"Huimin Qian, Chuang Li, Yanan Lu, Xue Li, Jianping Zhang, Junyi Zhao, Keyan Wu, Yanyan Zhang, Kun Cheng, Daowen Wang, Pengyu Song, Na Liu, Wenming Zheng","doi":"10.1111/pbi.70402","DOIUrl":"https://doi.org/10.1111/pbi.70402","url":null,"abstract":"Wheat leaf rust, caused by <jats:italic>Puccinia triticina</jats:italic> (<jats:italic>Pt</jats:italic>), threatens global wheat production, with yield losses further exacerbated by the pathogen's evolving virulence. Although Syg1/Pho81/Xpr1 (SPX) domain‐containing proteins are known regulators of phosphate homeostasis, their involvement in plant–pathogen interactions remains largely unexplored. We demonstrated that <jats:italic>TaSPX3</jats:italic>, a wheat SPX family gene, is rapidly induced during early <jats:italic>Pt</jats:italic> infection and flg22 treatment. Genetic evidence indicates that <jats:italic>TaSPX3</jats:italic> is a positive regulator of rust resistance, with knockdown lines showing increased susceptibility and overexpression lines exhibiting enhanced resistance. Using yeast two‐hybrid screening, we identified TaDi19‐1D, a zinc finger transcription factor, as a direct TaSPX3 interactor. TaDi19‐1D functions as a negative immune regulator by suppressing the expression of pathogenesis‐related (PR) genes (<jats:italic>TaPR1</jats:italic>, <jats:italic>TaPR2</jats:italic>, <jats:italic>TaPR5</jats:italic>) through direct promoter binding. TaSPX3 counteracts this repression by physically interacting with TaDi19‐1D, thereby derepressing PR gene expression and boosting wheat resistance to <jats:italic>Pt</jats:italic>. Our findings revealed a novel TaSPX3–TaDi19 regulatory module that fine‐tunes <jats:italic>TaPRs</jats:italic> expression, providing mechanistic insights into pattern‐triggered immunity (PTI) and potential genetic targets for breeding durable broad‐spectrum disease‐resistant wheat varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"20 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.","authors":"Kim N Janssen,Paul Bolay,Adrian Tüllinghoff,Jörg Toepel,Daniel Spindler,Bruno Bühler,Pia Lindberg","doi":"10.1111/pbi.70395","DOIUrl":"https://doi.org/10.1111/pbi.70395","url":null,"abstract":"In light of the looming climate crisis, a key cornerstone for a sustainable bioeconomy is photosynthetic production of chemicals and fuels from CO2 and water, powered by sunlight. Isoprene is a five-carbon volatile hydrocarbon with industrial use as a feedstock for rubber production and chemical synthesis and is, at present, generated from crude oil sources. It is, however, possible to produce isoprene using photoautotrophic microorganisms such as cyanobacteria, heterologously expressing the plant enzyme isoprene synthase. Here, we have employed diverse metabolic engineering strategies to develop new strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 capable of high-level isoprene production from CO2, and have characterised the resulting strains regarding growth, stability and productivity. The new isoprene-producing strains address several challenges in large-scale photobiotechnological production such as genetic and metabolic stability, biosafety and thermotolerance. Moreover, we tested photosynthetic terpenoid production in photobioreactors under process-relevant conditions, achieving the highest volumetric productivities reported so far for a terpene or terpenoid product in cyanobacteria, reaching 148 mg L-1 day-1. Furthermore, we identified and discussed process limitations, laying the foundation for further strain and process engineering towards highly efficient and stable cyanobacterial hydrocarbon production at large scale without selection pressure.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"158 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of Transgenes and Evaluation of Yield Penalties in Genome Edited Bacterial Blight Resistant Rice Varieties","authors":"Eliza P. I. Loo, José C. Huguet‐Tapia, Michael Selvaraj, Melissa Stiebner, Britta Killing, Marcel Buchholzer, Van Schepler‐Luu, Thomas Hartwig, Sandra P. Valdéz Gutierrez, Madlen I. Rast‐Somssich, Boris Szurek, Joe Tohme, Paul Charraviaga, Frank F. White, Bing Yang, Wolf B. Frommer","doi":"10.1111/pbi.70332","DOIUrl":"https://doi.org/10.1111/pbi.70332","url":null,"abstract":"Bacterial blight (BB) of rice, caused by <jats:styled-content style=\"fixed-case\"><jats:italic>Xanthomonas oryzae</jats:italic></jats:styled-content> pv. <jats:italic>oryzae</jats:italic> (Xoo), is one of the major drivers of yield losses in Africa and Asia. Xoo secretes TAL‐effectors (TALe) that induce host SWEET sucrose uniporter by binding to the effector binding element (EBE) of <jats:italic>SWEET</jats:italic> promoters, likely required for Xoo reproduction and virulence. We had multiplex edited the EBEs of three SWEET genes to prevent TALe binding, producing genome‐edited (GE'd) rice mega‐varieties (IR64, Ciherang‐Sub1 for Asia and Komboka for Africa) that were resistant to a wide spectrum of Xoo strains. Here, we report comprehensive analyses of the GE'd lines, including evaluation of agronomic performance in multi‐location multi‐season experimental field plots under different fertilisation regimes and tests for the presence/absence of foreign DNA/transgene in the offspring of GE'd lines (IR64‐BC1T6, Ciherang‐Sub1‐BC1T5, Komboka‐T3). Various strategies were evaluated, including herbicide tolerance, PCR, DNA gel blotting, whole genome sequencing (WGS), and specific tests stipulated by country‐specific biosafety guidelines. Different WGS technologies were evaluated and also used to identify the heritability of the edits, single‐nucleotide polymorphisms (SNPs), and insertions/deletions (indels) that might have resulted from somaclonal variation and potential GE‐induced off‐target mutations. Complete genome reference sequences for the parental lines IR64, Ciherang‐Sub1, and Komboka are provided. In the field experiments, the GE'd lines did not show performance defects. Together, the results indicate that select GE'd lines do not contain foreign DNA or transgene fragments and fulfil the requirements for treatment equivalent to classical breeding lines in countries such as India and Kenya.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA‐Induced Gene Silencing (MIGS): A Tool for Multi‐Gene Silencing and Targeting Viruses in Plants","authors":"Marie‐Emilie A. Gauthier, Kylie Shand, Satomi Hayashi, Peter M. Waterhouse, Roberto A. Barrero, Felipe F. de Felippes","doi":"10.1111/pbi.70401","DOIUrl":"https://doi.org/10.1111/pbi.70401","url":null,"abstract":"Since its discovery, RNA interference (RNAi, also known as gene silencing) has been a key tool to downregulate gene expression in plants for a range of applications, including protection against viruses. Many of these applications require the silencing of multiple genes concomitantly. Multi‐gene silencing, however, can be difficult to achieve owing to challenges in generating single RNAi constructs targeting unrelated genes or due to molecular instability linked to those constructs. Here, we show that microRNA‐induced gene silencing (MIGS) can overcome many of these limitations and can be an important tool for multi‐gene silencing in plants. We demonstrate how MIGS targeting several genes enhances the RNAi‐based inhibition of one or more viruses. We also define several key features for optimising the use of MIGS in plants, including modular design, effective targeting length and phased first‐base composition.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"107 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmmiR1432-ZmCML21-ZmPMA2 Module Affects Maize Low Phosphate Tolerance via Regulating Organic Acid Secretion.","authors":"Laming Pei,Yaqing Yang,Zhe Wang,Wencheng Duan,Ning Liu,Zhaohua Ding,Hui Li","doi":"10.1111/pbi.70385","DOIUrl":"https://doi.org/10.1111/pbi.70385","url":null,"abstract":"Phosphorus is indispensable in agricultural production. The growing global demand for food necessitates the development of crops with enhanced phosphorus utilisation efficiency. However, the molecular mechanisms coordinating phosphorus utilisation efficiency in plants remain incompletely characterised. MicroRNAs, pivotal regulators of plant developmental and physiological processes, have emerged as key targets for deciphering the regulatory networks underlying low phosphate (Pi) tolerance. Herein, we delineate the regulatory role of ZmmiR1432 in maize and elucidate its mechanistic basis in conferring low Pi tolerance. Suppression of ZmmiR1432 markedly improved tolerance to Pi deficiency via enhanced organic acid exudation, whereas its overexpression had the opposite effect. It is also indicated that ZmmiR1432 regulates low Pi tolerance through direct modulation of its target gene, ZmCML21, a calmodulin-like protein coding gene that also plays a key role in organic acid secretion and Pi-deficiency response. Metabolomic and transcriptomic analyses revealed that overexpression of ZmCML21 severely affected organic acid secretion and altered the expression of genes involved in the citrate cycle (TCA cycle). Furthermore, it is demonstrated that ZmCML21 directly interacts with plasma membrane H+-ATPase (ZmPMA2). Overexpression of ZmPMA2 phenocopied the ZmmiR1432 knockdown plants and ZmCML21 overexpression plants. Collectively, our findings uncover a ZmmiR1432-ZmCML21 regulatory module that governs low Pi tolerance by modulating ZmPMA2 activity, thereby influencing organic acid secretion and ultimately determining Pi use efficiency. These results provide mechanistic insights and actionable genetic targets for improving Pi use efficiency in maize through molecular breeding and genetic engineering.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The RNA m6A Methyltransferase PvFIP37 Activates Vacuole Transport and Glutathione Detoxification Pathways to Increase Switchgrass Cd Tolerance.","authors":"Mengzhuo Lin,Lei Wang,Bowen Liu,Huayue Liu,Wenwu Qian,Die Zhou,Hui Zang,Binglin Hong,Yunwei Zhang,Jishan Jiang","doi":"10.1111/pbi.70377","DOIUrl":"https://doi.org/10.1111/pbi.70377","url":null,"abstract":"Cadmium (Cd) pollution is a growing global issue that poses significant threats to ecosystems and human health. N6-methyladenosine (m6A) plays an essential role in regulating plant growth and stress responses, but the biological functions and regulatory mechanisms of m6A core enzymes under Cd stress remain largely unexplored. In this study, we demonstrate that PvFIP37 (WTAP human homologue protein) enhances Cd tolerance in switchgrass by activating PvYSL7, PvYSL17, PvABCC4 and PvABCC9 to transport Cd to the vacuoles. Additionally, PvFIP37 stabilises PvGST3 and PvGSTU6 within the glutathione S-transferase detoxification pathway, leading to increased GST activity and a higher GSH/GSSG ratio, thus alleviating oxidative damage in switchgrass under Cd stress. We have further shown that PvMTA interacts with PvFIP37 and targets the same set of genes as PvFIP37 to enhance switchgrass Cd tolerance. In summary, our study illustrates a novel mechanism of m6A methyltransferase-mediated Cd tolerance in switchgrass and provides valuable genetic resources for breeding Cd tolerant plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"81 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single Cell Multi‐Omics Reveals Rare Biosynthetic Cell Types in the Medicinal Tree Camptotheca acuminata","authors":"Van‐Hung Bui, Joshua C. Wood, Brieanne Vaillancourt, John P. Hamilton, Lemor H. Carlton, Thu‐Thuy T. Dang, C. Robin Buell, Chenxin Li","doi":"10.1111/pbi.70386","DOIUrl":"https://doi.org/10.1111/pbi.70386","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"20 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}