Plant Biotechnology Journal最新文献

{"title":"Expression of a bacterial salicylate hydroxylase gene NahG impedes the expression of PAMP-triggered immunity-related genes in plants.","authors":"Yuan Wang,Youwei Ouyang,Limin Jiang,Jiajing Xiao,Gang Yu","doi":"10.1111/pbi.70108","DOIUrl":"https://doi.org/10.1111/pbi.70108","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haploid induction in sweet potato by activating the AP2/ERF family transcription factor IbBBM.","authors":"Wenpeng Song,Yixuan Zhu,Junyao Zheng,Hong Zhai,Shaozhen He,Huan Zhang,Ning Zhao,Qingchang Liu,Shaopei Gao","doi":"10.1111/pbi.70137","DOIUrl":"https://doi.org/10.1111/pbi.70137","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmFER1, a soybean ferritin, enhances tolerance to salt stress and root rot disease and improves soybean yield.","authors":"Yanzheng Zhang,Shuhan Liu,Xiaoyue Liang,Jiqiang Zheng,Xiangpeng Lu,Jialiang Zhao,Haibin Li,Yuhang Zhan,Weili Teng,Haiyan Li,Yingpeng Han,Xue Zhao,Yongguang Li","doi":"10.1111/pbi.70102","DOIUrl":"https://doi.org/10.1111/pbi.70102","url":null,"abstract":"The plant stress response mechanism is activated by biotic and abiotic stresses, but its continuous activation typically affects growth. The role of ferritin in regulating biomass accumulation has been extensively characterized in diverse plant species; however, the underlying mechanisms through which it contributes to salt stress tolerance and Fusarium resistance remain poorly understood. Here, we confirm that overexpression of ferritin leads to iron accumulation and Fe3+ sequestration in both aboveground and roots, activating the iron uptake and transport system. More importantly, GmFER1 enhances salt stress tolerance and Fusarium resistance. First, GmFER1 is localized in chloroplasts and significantly induced by salt stress and Fusarium infection. Overexpression of GmFER1 increases soybean yield per plant by enhancing net photosynthetic rate and Rubisco enzyme activity, without activating the reactive oxygen scavenging mechanism. Under salt stress, GmFER1 enhances resistance by improving the activities of SOD and CAT enzymes, as well as Na+ efflux capacity. Under Fusarium infection, GmFER1 enhances resistance to the pathogen by boosting antioxidant capacity. Moreover, iron-deficiency tests revealed that increased CAT and SOD activities under salt stress are linked to iron ions accumulation. Lastly, we analysed the effects of GmFER1 gene variation on salt tolerance, disease resistance and 23 agronomic traits related to yield and quality. Further analysis of GmFER1 gene variation revealed that the Hap2 haplotypes could potentially enhance salt resistance, disease resistance, pod number and oil content in soybean. Our research offers a new way to reduce growth penalties while boosting plant resistance to salt stress and Fusarium infection.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CgABR1-CgFAD negatively regulates the fruit neck length in citrus.","authors":"Shengjun Liu,Xuejun Bei,Yawei Li,Xiang Gao,Fusheng Wang,Xiaoyan An,Qingjiang Wu,Jinmei Huang,Lixia Lu,Hongming Liu,Chunrui Long,Yuantao Xu,Xia Wang,Qiang Xu,Shaohua Wang","doi":"10.1111/pbi.70111","DOIUrl":"https://doi.org/10.1111/pbi.70111","url":null,"abstract":"Pummelo (Citrus maxima) is a fundamental species of Citrus which contributes to most of the cultivated citrus, including sweet orange, lemon and etc. The fruit neck is a structural feature of pummelo, and a long fruit neck reduces the edible rate of the fruit. In this study, we assembled a telomere-to-telomere (T2T) gap-free reference genome for the typical short fruit neck cultivar, 'Pingshan' pummelo, and a chromosome-level genome for the typical long fruit neck cultivar, 'Shatian' pummelo. Here, we used a segment population derived from a cross between a long fruit neck cultivar ('Guanxi' pummelo) and a short fruit neck ('Pingshan' pummelo) cultivar to map the determinant controlling the fruit neck length. We identified a strong peak on chromosome 1 within the 27.5-30.5 Mb physical region and found a 52 bp deletion linked with the fruit neck length. Moreover, by combining RNA sequencing data of the fruit neck development and variation analysis, we identified two genes, one encodes ethylene-responsive transcription factor (CgABR1) and the other encodes FAD-dependent urate hydroxylase (CgFAD). Genetic transformation confirmed that overexpression of CgABR1 and CgFAD can inhibit fruit neck length. DNA affinity purification sequencing, electrophoretic mobility shift assays and dual-LUC reporter assays demonstrated that CgABR1 can activate the expression of CgFAD by directly binding to its promoter. In summary, we assembled a T2T gap-free genome for pummelo and identified the key genes for fruit neck length in citrus, offering an important resource and new genes for citrus genetic improvement and breeding programs.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"30 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins.","authors":"Elia Lacchini,Tongtong Qu,Tessa Moses,Alexander N Volkov,Alain Goossens","doi":"10.1111/pbi.70122","DOIUrl":"https://doi.org/10.1111/pbi.70122","url":null,"abstract":"The limited cytosolic delivery of DNA and protein-based therapeutics due to endosomal entrapment reduces drug efficacy, increasing treatment costs and possible side effects in human and veterinary medicine as a consequence of higher administered dosages. Plant-derived triterpenoid saponins, specifically those with endosomal escape-enhancing (EEE) properties, have shown promise in overcoming this limitation by disrupting endosomal membranes. QS-21, a well-known EEE saponin, has been used as an adjuvant in vaccines, and recent studies have elucidated its biosynthetic pathway. However, EEE saponins are typically present as minor compounds in plants, posing challenges for their large-scale production and purification. Here we investigated the possibility of engineering SO1861 production, an EEE saponin from Saponaria officinalis, using heterologous gene expression in Gypsophila elegans hairy roots, a plant species known to synthesize structurally related saponins. Via S. officinalis transcriptomics, we identified jasmonate-responsive saponin biosynthetic genes, and three cytochrome P450s (CYP450s) involved in C23, C28 and C16 oxidations were characterised. Heterologous expression of these CYP450s in G. elegans hairy roots successfully altered the saponin profile, with notable increases in SO1861 precursors in lines expressing the C23-oxidases SoCYP72A984 and SoCYP72A1003. Interestingly, expression of only SoCYP72A1003, a non-canonical C23 oxidase, resulted in the accumulation of a compound matching the SO1861 standard, suggesting the activation of a potentially latent pathway and of silent enzymes in a novel combination. This work underscores the potential of engineering strategies in heterologous plant systems to steer triterpenoid saponin biosynthetic pathways and suggests new avenues for producing high-value EEE saponins.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"37 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-induced gene silencing of the amino acid biosynthesis gene acetolactate synthase of Phytophthora infestans caused strong enhanced late blight resistance of potato in the field","authors":"Nora Temme, Tobias Haehre, Clarissa Boyher, Lydia Hoppe, Colin Davenport, Zach Stumo, Kai Prenzler, Anja Maeser, Maike Pflugmacher, Kerstin Koch, Dietmar J. Stahl","doi":"10.1111/pbi.70133","DOIUrl":"https://doi.org/10.1111/pbi.70133","url":null,"abstract":"Late blight caused by <i>Phytophthora infestans</i> is the most serious disease of potatoes. Here we present the effectiveness of the host-induced gene silencing (HIGS) technology against an amino acid biosynthesis gene of the pathogen to increase the resistance against the plant-infecting oomycete in the field. A RNAi hairpin construct directed against the acetolactate synthase (<i>ALS</i>) gene of <i>Phytophthora infestans</i> was transferred into potato. HIGS-ALS potato lines displayed efficient target gene silencing revealed by a luciferase reporter gene assay. Plant-derived siRNAs targeting the oomycete's <i>ALS</i> gene were detected by small RNA sequencing. <i>ALS</i> gene expression of <i>P. infestans</i> was reduced during the early infection stages of HIGS-ALS potatoes, as shown by qRT-PCR. HIGS-ALS plants revealed an enhanced late blight resistance in detached leaf assays. ALS gene silencing also conferred strong enhanced late blight resistance to the HIGS lines in trials under near-field conditions in Europe and in field trials in the USA against European and US <i>P. infestans</i> isolates, respectively. These results demonstrated the value of the HIGS technology for the development of a new quantitative resistance source for potato against <i>Phytophthora infestans</i>.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"44 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing plant size for vertical farming by editing stem length regulators","authors":"Yoonseo Lim, Myeong-Gyun Seo, Jiwoo Lee, Seungpyo Hong, Jeong-Tak An, Ho-Young Jeong, Hong-Il Choi, Woo-Jong Hong, Chanhui Lee, Soon Ju Park, Choon-Tak Kwon","doi":"10.1111/pbi.70129","DOIUrl":"https://doi.org/10.1111/pbi.70129","url":null,"abstract":"Vertical farming offers the advantage of providing a stable environment for plant cultivation, shielding them from adverse conditions such as climate change. For fruit-harvesting plants like tomato, vertical farming necessitates the optimization of plant growth and architecture. The <i>gibberellin 3-oxidase</i> (<i>GA3ox</i>) genes encode gibberellin 3-oxidases responsible for activating GA within the pathway and modulating stem length. Among the five <i>SlGA3ox</i> genes, we targeted the coding regions of three <i>SlGA3ox</i> genes (named <i>SlGA3ox3</i>, <i>SlGA3ox4</i> and <i>SlGA3ox5</i>) using multiplex CRISPR genome editing. The <i>slga3ox4</i> single mutants exhibited a slight reduction in primary shoot length, leading to a smaller stature. In contrast, the <i>slga3ox3</i> and <i>slga3ox5</i> single mutants showed subtle phenotypic changes. Notably, the <i>slga3ox3 slga3ox4</i> double mutants developed a more compact shoot architecture with minor physiological differences, potentially making them suitable for vertical farming applications. We observed a correlation between total yield and plant size across all genotypes through multiple yield trials. Observations from vertical farm cultivation revealed that <i>slga3ox3 slga3ox4</i> plants possess a markedly compact plant size, offering potential benefits for space-efficient cultivation. Our research suggests that targeted manipulation of hormone biosynthetic genes can effectively tailor plant architecture for vertical farming.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"11 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice","authors":"Jia Guo, Hongshan Zhang, Mengyu Fan, Yongkang Xiao, Yongshuang Zhu, Congping Chen, Hao Shu, Miaomiao Wei, Yating Luo, Xiaorong Yang, Yue Liu, Jinze Xu, Ke Zhao, San Wang, Bin Yang, Changhui Sun, Xiaojian Deng, Pingrong Wang","doi":"10.1111/pbi.70128","DOIUrl":"https://doi.org/10.1111/pbi.70128","url":null,"abstract":"Brassinosteroids (BRs) are a crucial class of plant hormones and regulate many important agronomic traits in crops. In Arabidopsis (<i>Arabidopsis thaliana</i>), BIR3 interacts with the BR receptor BRI1 and coreceptor BAK1 to negatively regulate BR signalling. In contrast, OsBIR3 interacts with OsBRI1 and OsBAK1 to positively regulate BR signalling in rice (<i>Oryza sativa</i>). However, our understanding of OsBIR3 remains incomplete. In this study, we isolated a <i>reduced upper branch</i> (<i>rub1</i>) mutant of rice, exhibiting a significant reduction in grain number. The causal gene for the mutant phenotype was <i>LOC_Os04g41030</i> (<i>OsBIR3</i>). OsBIR3 interacts with both OsBRI1 and the remorin protein OsREM4.1, but interactions of the mutated Osbir3 with both OsBRI1 and OsREM4.1 were decreased. Furthermore, BL interferes with the interaction of OsBIR3 with OsBRI1, but promotes the interaction of OsBIR3 with OsREM4.1. Overexpression of <i>OsREM4.1</i> and <i>OsBRI1</i> individually in the <i>rub1</i> mutant caused an exacerbation of the mutant phenotype. Additionally, OsBIR3 interacts with Brd2, involving BR biosynthesis in the early stage, and the interaction of the mutated brd2 with wild-type OsBIR3 was increased. Besides, BL promotes the interaction between OsBIR3 and Brd2. Collectively, the data indicate that OsBIR3 plays a key role in maintaining the homeostasis of OsBRI1, OsREM4.1, and Brd2 at their respective protein levels. This work provides insight into the roles of OsBIR3 in BR signalling and biosynthesis pathways of rice.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The KNOX transcription factor ClSP activates ClAPRR2 to regulate dark green stripe formation in watermelon.","authors":"Zhen Yue,Yanan Fu,Xue Dai,Yingda Chen,Chenxi Guo,Ruiqing Zhang,Xin Huang,Mengjiao Feng,Xing Yan,Zhongyuan Wang,Rong Yu,Shi Liu,Hao Li,Xian Zhang,Li Yuan,Chunhua Wei","doi":"10.1111/pbi.70127","DOIUrl":"https://doi.org/10.1111/pbi.70127","url":null,"abstract":"As a prominent external feature of watermelon, the stripe pattern exhibits remarkable phenotypic diversity, directly impacting commercial value through consumer preference. However, the genetic and molecular mechanisms underlying this important agronomic trait in watermelon remain poorly understood. In this study, we discovered that the total chlorophyll content in dark green stripes (DGS) was significantly higher than that in light green stripes (LGS) or reticular green stripes (RGS). Moreover, the number and size of chloroplasts were significantly increased in the DGS. Genetic analysis identified the KNOX TF ClSP as the most likely candidate for regulating watermelon dark green stripe formation, whose functional disruption substantially impaired chlorophyll biosynthesis and chloroplast development, converting dark green stripes into reticulate stripes. Through transcriptome analysis, we identified approximately 94 differently expressed genes (DEGs) that contain the KNOX TF binding cis-element 'TGAC' in their promoters. Among these genes, the expression pattern of ARABIDOPSIS PSEUDO RESPONSE REGULATOR 2-LIKE (APRR2-like) TF ClAPRR2 closely mirrored that of ClSP, displaying significantly down-regulated transcriptional expression in LGS compared to DGS. Utilizing Y1H, GUS activity, DLR and EMSA assays, we confirmed that ClSP activates the transcriptional activity of ClAPRR2 through promoter binding. Collectively, we propose a potential working model for the ClSP-ClAPRR2 module, which regulates the chlorophyll synthesis and chloroplast development in watermelon fruits, providing new insights into the mechanisms underlying stripe pattern formation.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"123 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of β‐galactosidases along the secretory pathway of Nicotiana benthamiana that collectively hamper engineering of galactose‐extended glycans on recombinant glycoproteins","authors":"Alex van der Kaaij, Myrna J. M. Bunte, Lisa Nijhof, Sanaz Mokhtari, Hein Overmars, Arjen Schots, Ruud H. P. Wilbers, Pieter Nibbering","doi":"10.1111/pbi.70126","DOIUrl":"https://doi.org/10.1111/pbi.70126","url":null,"abstract":"SummaryGlycosylation is an important aspect for many biopharmaceuticals, including vaccines against parasitic helminths. Plants, especially <jats:italic>Nicotiana benthamiana</jats:italic>, have proven to be excellent production hosts for biopharmaceuticals with tailor‐made glycosylation. If desired, galactosylation can be introduced on biopharmaceuticals through co‐expression of the appropriate glycosyltransferase. However, achieving homogenous glycoforms with terminal galactose residues remains difficult as native <jats:italic>N. benthamiana</jats:italic> β‐galactosidases (NbBGALs) truncate these glycans. Recently, the first NbBGAL has been identified, but a knockout line was insufficient to achieve near complete galactosylation, suggesting that other enzymes could have similar activity. In this study, we selected 10 NbBGALs for further investigation into subcellular localization, <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> activity against β1,4‐linked galactose on N‐glycans and β1,3‐linked galactose on O‐glycans. We show that NbBGAL3B is localized in the apoplast and has similar specificity for β1,4‐linked galactose on N‐glycans as the previously identified NbBGAL1. In contrast, none of the selected NbBGALs cleaved β1,3‐linked galactose from O‐glycans besides BGAL1. In addition, we provide a novel strategy to achieve near complete galactosylation on galactosidase‐prone glycoproteins by using the protective capacity of the Lewis X motif and subsequent removal of the antennary fucose residues. Taken together, our results provide a broad view of the ability of NbBGALs to cleave galactoses and have identified NbBGAL3B as the second major contributor of undesired β‐galactosidase activity while engineering N‐glycans. This work lays the foundation for generating knockout lines that are devoid of undesired NbBGALs and therefore do not hamper the production of recombinant glycoproteins with galactose‐extended glycans.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}