Plant Biotechnology Journal最新文献

{"title":"Natural allelic variation of basic helix–loop–helix transcription factor 25 regulates carotenoid biosynthesis in sweet potato","authors":"Zihao Wei, Meiqi Shang, Zhicheng Jiang, Hong Zhai, Shihan Xing, Zhen Wang, Shaozhen He, Shaopei Gao, Ning Zhao, Huan Zhang, Qingchang Liu","doi":"10.1111/pbi.70086","DOIUrl":"https://doi.org/10.1111/pbi.70086","url":null,"abstract":"Carotenoid-rich orange-fleshed sweet potato (OFSP) is an important staple diet and source of nutrition in developing countries, including Africa and Asia. However, the regulation of carotenoid biosynthesis remains to be better understood. A natural allelic variation closely linked to carotenoid biosynthesis was identified in the promoter region of the <i>IbbHLH25</i> gene that encodes a basic helix–loop–helix (bHLH) transcription factor, by transcriptome and haplotype analyses of different flesh colour sweet potato accessions. An 86-bp deletion reduced the transcription of the <i>IbbHLH25</i> promoter in white- and yellow-fleshed sweet potatoes; however, the deletion was absent in OFSP. <i>IbbHLH25</i> was highly expressed in the storage roots of carotenoid-rich sweet potato. The overexpression of <i>IbbHLH25</i> significantly increased the carotenoid contents (by 2.5-fold–6.0-fold) and proportions, especially β-carotene and β-cryptoxanthin; their contents increased by 21.2-fold–55.7-fold and 4.6-fold–9.5-fold, respectively, and their proportions increased by 48.5% and 13.0%, respectively, and the silencing of <i>IbbHLH25</i> had opposite effects. IbbHLH25 formed heterodimers with IbbHLH66 to directly and synergistically activate the transcription of carotenoid biosynthesis key genes <i>IbGGPPS</i>, <i>IbLCYB</i> and <i>IbBCH</i>. The overexpression of <i>IbbHLH66</i> significantly increased the carotenoid contents (by 2.3-fold–3.8-fold) and proportions, especially β-carotene and β-cryptoxanthin; their contents increased by 15.2-fold–25.6-fold and 3.1-fold–5.1-fold, respectively, and their proportions increased by 31.1% and 9.6%, respectively. These findings expand our understanding of bHLHs in regulating carotenoid biosynthesis and suggest additional roles in affecting carotenoid component proportions, providing candidate genes for nutritional biofortification of agricultural products.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted insertion of large DNA fragments through template-jumping prime editing in rice","authors":"Fei Li, Haonan Hou, Minglei Song, Zhen Chen, Ting Peng, Yanxiu Du, Yafan Zhao, Junzhou Li, Chunbo Miao","doi":"10.1111/pbi.70087","DOIUrl":"https://doi.org/10.1111/pbi.70087","url":null,"abstract":"&lt;p&gt;Targeted insertion of large DNA fragments holds great promise in crop breeding but is extremely challenging in plants. Prime editing (PE) can efficiently install small genomic insertion through replacement but faces challenges in mediating insertion of &gt;100 bp. To enable larger insertion, a paired PE strategy termed GRAND editing was developed in human cells. In plants, genomic insertions of up to 135 bp were achieved through GRAND editing (Xu &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;), but no plants with larger targeted insertion were generated through PE-mediated replacement.&lt;/p&gt;\u0000&lt;p&gt;Here, we attempted to insert three truncated promoters, including 207-bp &lt;i&gt;pGluB4&lt;/i&gt;, 204-bp &lt;i&gt;p10kDa&lt;/i&gt;, and 206-bp &lt;i&gt;p16kDa&lt;/i&gt;, to the 5′ UTRs of &lt;i&gt;OsC1&lt;/i&gt;, &lt;i&gt;OsB2&lt;/i&gt;, and &lt;i&gt;OsB1&lt;/i&gt;, respectively, through PE. An improved prime editor termed ePPEplus (Ni &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;) and epegRNA (pegRNA with a tevepreQ1 motif at the 3′ terminus) were used in the PE assays of this study. Expressions of the ePPEplus and epegRNAs were driven by &lt;i&gt;ZmUbi&lt;/i&gt; and eCmYLCV promoters (eCmYLCV, 35S enhancer-CmYLCV promoter), respectively. All PE assays in this study were conducted in a &lt;i&gt;japonica&lt;/i&gt; cultivar termed Heixiangnuo (HXN). Firstly, we conducted the insertion editing using GRAND editing but detected no targeted insertions in the GRAND editing transgenic plants (Table S1; see Supplemental Methods and Figures S1 and S2 for the design).&lt;/p&gt;\u0000&lt;p&gt;Recently, a PE technology termed template-jumping PE (TJ-PE) was developed for large insertion in human cells (see Figure S3 for the mechanism) (Zheng &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). TJ-PE pegRNA (TJ-pegRNA) contains one reverse transcriptase template (RTT) and two primer binding sites (PBSs), with one PBS matching the pegRNA target and another matching the nicking gRNA target (Figure S3). TJ-PE could mediate 200–800-bp insertion in replacement of the fragment between the two TJ-PE nicks in human cells. Thus, we also conducted the insertion editing using TJ-PE with the same target sites as the above GRAND editing (Figure 1a and Figure S4). In the TJ-PE assays, the TJ-epegRNAs were expressed with pre-tRNA and hepatitis delta virus ribozyme (HDV) processing systems to generate mature epegRNAs (Figure S5). Each TJ-epegRNA expression cassette and the corresponding nicking gRNA cassette were constructed in a binary vector with an ePPEplus cassette to generate one vector for each insertion editing (Figure S5). With each of the TJ-PE vectors, 97–137 transgenic plants were generated through &lt;i&gt;Agrobacterium&lt;/i&gt;-mediated transformation. Among these TJ-PE transgenic plants, only two edited plants with truncated &lt;i&gt;pGluB4&lt;/i&gt; insertions at &lt;i&gt;OsC1&lt;/i&gt; (one with 149-bp &lt;i&gt;pGluB4&lt;/i&gt; insertion and another with 139-bp &lt;i&gt;pGluB4&lt;/i&gt; insertion) were identified, and no edits were detected in other transgenic plants (Table S2; Figure S6a–c). The 149- and 139-bp insertions occurred at &lt;i&gt;OsC1&lt;/i&gt; TJ-epegRNA nicking site with a p","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant cell-based platform for the expression of complex proteins with fucose-reduced sialylated N-glycans","authors":"Saeideh Dianatkhah, Benjamin Kogelmann, Stanislav Melnik, Florian Eminger, Somanath Kallolimath, Lin Sun, Delia Sumesgutner, Michael W. Traxlmayr, Markus Sack, Eva Stoger, Herta Steinkellner","doi":"10.1111/pbi.70044","DOIUrl":"https://doi.org/10.1111/pbi.70044","url":null,"abstract":"&lt;p&gt;Sialylated N-glycans are widely distributed in vertebrates and represent the dominant glycoform of many human plasma proteins (Miura &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;). Although knowledge of the diverse effects of this glycan formation is rapidly increasing, full understanding of its biological significance remains elusive (Lewis &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). A major reason for this is the difficulty in controlling sialylation in production processes.&lt;/p&gt;\u0000&lt;p&gt;Plants are considered as an effective platform for the production of recombinant proteins used in basic research or for various applications (Eidenberger &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). The platform has recently been extended by so-called plant cell packs (PCPs), three-dimensional, porous plant cell aggregates derived from plant suspension cells. The approach enables high-throughput transient expression of foreign genes and upscaling for subsequent protein purification and characterization (Rademacher &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;) and (WO2013113504).&lt;/p&gt;\u0000&lt;p&gt;An important advantage of plant-based expression is the synthesis of N-glycans similar to mammalian cells. Usually, secreted plant glycoproteins are decorated with GlcNAc-terminating complex N-glycans carrying a plant-specific core xylose and α1,3-fucose, so-called GnGnXF structures. Extensive engineering in &lt;i&gt;N. benthamiana&lt;/i&gt;, that is, the inactivation of genes responsible for the addition of plant-specific core xylose and fucose, in combination with the overexpression of six foreign genes involved in the human sialylation pathway, resulted in the generation of a plant line (ΔXTFT&lt;sup&gt;Sia&lt;/sup&gt;) that synthesizes sialylated N-glycans (Eidenberger &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; Kallolimath &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2016&lt;/span&gt;). One shortcoming of the ΔXTFT&lt;sup&gt;Sia&lt;/sup&gt; line is the lower seed production, which makes maintenance and widespread use difficult.&lt;/p&gt;\u0000&lt;p&gt;Here we used hypocotyl of ΔXTFT&lt;sup&gt;Sia&lt;/sup&gt; plants as starting material for callus induction, applying a similar method as described recently (Sukenik &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2018&lt;/span&gt;) (Figure S1a, ‘Materials and methods’ section). After tissue dedifferentiation, calli were maintained on semi-solid media by monthly subculturing. Portions of independent calli, PCR-screened for the presence of one of the six foreign glycosylation genes for sialylation (Figures S1a and S1b), were used to initiate suspension cultures, which were maintained for several passages to select for rapid growth (Figure S1a). For the generation of PCPs (Rademacher &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;), cells were separated from excess cultivation medium by slow centrifugation in ultrafiltration spin-columns The resulting semi-dry porous cell aggregates (called plant cell packs, PCP&lt;sup&gt;Sia&lt;/sup&gt;; Figure S1a) were monitored for expression of recombinant fluorescent protein. PCP&lt;sup&gt;Sia&lt;/sup&gt; were incubated with &lt;i&gt;Rhizobium radiobacter&lt;/i&gt; (formerly &lt;i&gt;Agrobacterium tumefaciens&lt;/i&gt;) suspension cul","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"99 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERF100 regulated by ERF28 and NOR controls pectate lyase 7, modulating fig (Ficus carica L.) fruit softening","authors":"Zhiyi Fan, Yuan Wang, Yanlei Zhai, Xiaojiao Gu, Kairong Sun, Dan Zhao, Jinying Wang, Pinqi Sun, Hantang Huang, Jiajun He, Yining Wang, Moshe A. Flaishman, Huiqin Ma","doi":"10.1111/pbi.70085","DOIUrl":"https://doi.org/10.1111/pbi.70085","url":null,"abstract":"The mechanism regulating fruit textural changes has not been fully elucidated. Transcription factor FcERF100 showed rapid transcription repression during drastic texture loss in fig (<i>Ficus carica</i> L.) fruit ripening. Transient overexpression of <i>FcERF100</i> delayed fig fruit softening and significantly decreased the transcript abundance of a key cell wall-modifying pectate lyase gene, <i>FcPL7</i>. Yeast one-hybrid (Y1H) assay, chromatin immunoprecipitation-qPCR, electrophoretic mobility shift assay (EMSA), and dual-luciferase reporter assay revealed that FcERF100 represses <i>FcPL7</i> transcription by direct promoter binding via GCC-box and DRE/CRT elements. Stable transgenic fig lines further verified FcERF100's inhibitory effect on <i>FcPL7</i> expression. We detected FcERF28 as an upstream element of <i>FcERF100</i> by Y1H and EMSA, revealing its binding to, and activation of <i>FcERF100</i> by dual-luciferase assay. Taken together, the FcERF28–FcERF100 transcriptional cascade serves as a synergistic flow-limiting valve for FcPL7 abundance. We then identified a NAC transcription factor, FcNOR, using FcERF100 as the bait by yeast two-hybrid screening. <i>FcNOR</i> silencing retarded fig fruit softening, with decreased <i>FcPL7</i> transcript and pectate lyase activity. FcNOR interacted with FcERF100 to form a protein complex, attenuating FcERF100's transcriptional repression of <i>FcPL7</i>. Moreover, FcNOR bound directly to the promoter of <i>FcERF100</i> and inhibited its transcription. In addition, ethylene treatment upregulated <i>FcNOR</i> and <i>FcPL7</i> expression and downregulated <i>FcERF28</i> and <i>FcERF100</i> expression. Our findings reveal a novel FcERF100-centered regulatory complex and resolve how the complex achieves the necessary cell wall modification during an early stage of fruit growth and implements drastic softening at fruit ripening by modulating component proportions.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localising enzymes to biomolecular condensates increase their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana","authors":"Anya L. Lindström Battle, Angus W. Barrett, Mark D. Fricker, Lee J. Sweetlove","doi":"10.1111/pbi.70082","DOIUrl":"https://doi.org/10.1111/pbi.70082","url":null,"abstract":"SummaryThe establishment of <jats:italic>Nicotiana benthamiana</jats:italic> as a robust biofactory is complicated by issues such as product toxicity and proteolytic degradation of target proteins/introduced enzymes. Here we investigate whether biomolecular condensates can be used to address these problems. We engineered biomolecular condensates in <jats:italic>N. benthamiana</jats:italic> leaves using transient expression of synthetic modular scaffolds. The <jats:italic>in vivo</jats:italic> properties of the condensates that resulted were consistent with them being liquid‐like bodies with thermodynamic features typical of multicomponent phase‐separating systems. We show that recruitment of enzymes to condensates <jats:italic>in vivo</jats:italic> led to several‐fold yield increases in one‐ and three‐step metabolic pathways (citramalate biosynthesis and poly‐3‐hydroxybutyrate (PHB) biosynthesis, respectively). This enhanced yield could be for several reasons including improved enzyme kinetics, metabolite channelling or avoidance of cytotoxicity by retention of the pathway product within the condensate, which was demonstrated for PHB. However, we also observed a several‐fold increase in the amount of the enzymes that accumulated when they were targeted to the condensates. This suggests that the enzymes were more stable when localised to the condensate than when freely diffusing in the cytosol. We hypothesise that this stability is likely the main driver for increased pathway product production. Our findings provide a foundation for leveraging biomolecular condensates in plant metabolic engineering and advance <jats:italic>N. benthamiana</jats:italic> as a versatile biofactory for industrial applications.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"66 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular heterogeneity and immune responses to smut pathogen in sugarcane","authors":"Shoujian Zang, Qibin Wu, Dongjiao Wang, Zhenxiang Li, Tingting Sun, Xinlu Sun, Tianzhen Cui, Yachun Su, Haifeng Wang, Youxiong Que","doi":"10.1111/pbi.70084","DOIUrl":"https://doi.org/10.1111/pbi.70084","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"39 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal haploid induction in maize via mutation of Gamete Expression protein 1","authors":"Yuhui Sun, Lulu Hou, Chengcheng Li, Zijian Zhou, Jianyu Wu, Haidong Yu","doi":"10.1111/pbi.70081","DOIUrl":"https://doi.org/10.1111/pbi.70081","url":null,"abstract":"SummaryDoubled haploid (DH) technology, based on haploid induction (HI), is a crucial tool in enhancing crop‐breeding efficiency and has been successfully applied in various plant species. While many HI‐related genes have been identified using diverse strategies, the genetic basis and molecular mechanisms underlying HI remain incompletely understood. In this study, we present a novel system for inducing haploid offspring through targeted mutagenesis of the <jats:italic>Zea mays Gamete Expression protein 1</jats:italic> (<jats:italic>ZmGEX1</jats:italic>) gene in maize. Our findings reveal that <jats:italic>zmgex1</jats:italic> heterozygous plants (<jats:italic>zmgex1</jats:italic><jats:sup>+/−</jats:sup>) induce maternal haploids via self‐ and cross‐pollination as the female parent, with an average rate of 1.34%. This indicates that the haploid progeny is exclusively maternal in origin, carrying the maternal genome. We also demonstrate that <jats:italic>ZmGEX1</jats:italic> is expressed in both female spikelets and anthers, localizing to the cytoplasm, nucleus and endoplasmic reticulum. Although the transmission efficiency of the <jats:italic>zmgex1</jats:italic> allele is reduced in female gametophytes, <jats:italic>ZmGEX1</jats:italic> does not affect embryo sac development but influences fertilization. We propose that defective fusion of the sperm and egg nuclei may lead to haploid formation. Finally, a schematic that illustrates the potential application of the new gene <jats:italic>ZmGEX1</jats:italic> in maize breeding programs is proposed. Collectively, this study identifies <jats:italic>ZmGEX1</jats:italic> as a novel gene involved in maternal HI and provides a promising strategy for breeding improvements.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"75 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizobium rhizogenesA4‐derived strains mediate hyper‐efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants","authors":"Juan Carlos Lopez‐Agudelo, Foong‐Jing Goh, Sopio Tchabashvili, Yu‐Seng Huang, Ching‐Yi Huang, Kim‐Teng Lee, Yi‐Chieh Wang, Yu Wu, Hao‐Xun Chang, Chih‐Horng Kuo, Erh‐Min Lai, Chih‐Hang Wu","doi":"10.1111/pbi.70083","DOIUrl":"https://doi.org/10.1111/pbi.70083","url":null,"abstract":"SummaryAgroinfiltration, a method utilizing agrobacteria to transfer DNA into plant cells, is widely used for transient gene expression in plants. Besides the commonly used <jats:italic>Agrobacterium</jats:italic> strains, <jats:italic>Rhizobium rhizogenes</jats:italic> can also introduce foreign DNA into host plants for gene expression. While many <jats:italic>R. rhizogenes</jats:italic> strains have been known for inducing hairy root symptoms, their use for transient expression has not been fully explored. Here, we showed that <jats:italic>R. rhizogenes</jats:italic> A4 outperformed all other tested agrobacterial strains in agroinfiltration experiments on leaves of <jats:italic>Nicotiana benthamiana</jats:italic> and other solanaceous plants. By conducting an agroinfiltration screening in <jats:italic>N. benthamiana</jats:italic> leaves using various agrobacterial strains carrying the RUBY reporter gene cassette, we discovered that A4 mediates the strongest and fastest transient expression. Utilizing the genomic information, we developed a collection of disarmed and modified strains derived from A4. By performing vacuum infiltration assays, we demonstrated that these A4‐derived strains efficiently transiently transform 6‐week‐old <jats:italic>N. benthamiana</jats:italic> leaves, showing less sensitivity to the age of plants compared to the laboratory strain GV3101. Furthermore, we performed agroinfiltration using AS109, an A4‐derived disarmed strain, on the leaves of tomato, pepper, and eggplant. Remarkably, AS109 mediated transient gene expression on tested solanaceous plants more effectively than all the tested commonly used agrobacterial strains. This discovery paves the way for establishing <jats:italic>R. rhizogenes</jats:italic> A4‐derived strains as a new option for enhancing transient expression in <jats:italic>N. benthamiana</jats:italic> and facilitating the functional study of plant genes in other solanaceous species.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"2 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A large-scale gene regulatory network for rice endosperm starch biosynthesis and its application in genetic improvement of rice quality","authors":"Kunyong Huang, Feifei Lu, Pengfei Chen, Guiai Jiao, Haiyan Lin, Jian Zhang, Shaolu Zhao, Ruijie Cao, Gaoneng Shao, Zhonghua Sheng, Shikai Hu, Shaoqing Tang, Peisong Hu, Xiangjin Wei","doi":"10.1111/pbi.70079","DOIUrl":"https://doi.org/10.1111/pbi.70079","url":null,"abstract":"Rice (<i>Oryza sativa</i> L.) is one of the most important food crops. Starch is the main substance of rice endosperm and largely determines the grain quality and yield. Starch biosynthesis in endosperm is very complex, requiring a series of enzymes which are also regulated by many transcription factors (TFs). But until now, the large-scale regulatory network for rice endosperm starch biosynthesis has not been established. Here, we constructed a rice endosperm starch biosynthesis regulatory network comprised of 277 TFs and 15 starch synthesis enzyme-encoding genes (SSEGs) using DNA affinity chromatography/pull-down combined with liquid chromatography-mass spectrometry (DNA pull-down and LC–MS). In this regulatory network, each SSEG is directly regulated by 7–46 TFs. Based on this network, we found a new pathway ‘ABA-OsABI5-OsERF44-SSEGs’ that regulates rice endosperm starch biosynthesis. We also knocked out five TFs targeting the key amylose synthesis enzyme gene <i>OsGBSSI</i> in <i>japonica</i> rice ‘Nipponbare’ background and found that all mutants had moderately decreased amylose content (AC) in endosperm and improved eating and cooking quality (ECQ). Notably, the knockout of <i>OsSPL7</i> and <i>OsB3</i> improves the ECQ without compromising the rice appearance quality, which was further validated in the <i>indica</i> rice ‘Zhongjiazao17’ background. In summary, this gene regulatory network for rice endosperm starch biosynthesis established here will provide important theoretical and practical guidance for the genetic improvement of rice quality.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"183 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Somatic embryogenetic receptor kinase TaSERL2 regulates heat stress tolerance in wheat by influencing TaBZR2 protein stability and transcriptional activity.","authors":"Xiang-Yang Hao, Tai-Fei Yu, Chao-Jun Peng, Yi-Han Fu, Yu-Hui Fang, Yan Li, Zhao-Shi Xu, Jun Chen, Hai-Bin Dong, You-Zhi Ma, Wei-Gang Xu","doi":"10.1111/pbi.70045","DOIUrl":"https://doi.org/10.1111/pbi.70045","url":null,"abstract":"<p><p>Heat stress is a major factor limiting crop yield, a challenge intensified by climate change. Initial findings indicate that BES1/BZR1 may use heat shock to regulate plant thermal adaptability independently of BIN2-mediated brassinosteroid signalling, although the exact molecular mechanism remains unclear. In this study, we identified TaBZR2, a wheat gene whose expression showed a strong positive correlation with heat stress tolerance, based on transcriptome analysis of heat-tolerant wheat cultivars. Overexpression of TaBZR2 enhanced heat stress tolerance, while RNA interference of TaBZR2 reduced it. Further analysis revealed that TaBZR2 interacts with and is phosphorylated by wheat somatic embryogenesis receptor-like kinase 2 (TaSERL2). Overexpression of TaSERL2 reduced heat stress tolerance by promoting TaBZR2 degradation and inhibiting its regulation of wheat heat stress response genes. However, heat stress reduced the phosphorylation levels of both TaSERL2 and TaBZR2, lessening TaSERL2's inhibitory effect on TaBZR2 and enhancing the stability of TaBZR2. These results reveal that the TaSERL2-TaBZR2 module negatively regulates plant heat stress tolerance. This study expands the current model of heat stress responses and provides evidence for the role of BES1/BZR1 in heat stress regulation independent of brassinosteroid signalling.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}