Paula Rios Glusberger,Benjamin Merritt,Cheng Liu,Yu Wang,Janice Zale,Hao Wu,Michel Canton,Michael Braverman,Eric W Triplett,Zhonglin Mou
{"title":"Risk Evaluation and Molecular Characterisation of AtNPR1 Transgenic Citrus Lines Tolerant to Citrus Greening Disease.","authors":"Paula Rios Glusberger,Benjamin Merritt,Cheng Liu,Yu Wang,Janice Zale,Hao Wu,Michel Canton,Michael Braverman,Eric W Triplett,Zhonglin Mou","doi":"10.1111/pbi.70394","DOIUrl":"https://doi.org/10.1111/pbi.70394","url":null,"abstract":"Citrus greening disease, or Huanglongbing (HLB), has caused devastating losses to citrus production in Florida, with yields declining by over 90% since 2005. Despite extensive efforts, no sustainable solution has been widely effective. Here, transgenic 'Hamlin' sweet orange lines engineered to constitutively express the Arabidopsis NPR1 (AtNPR1) gene, a key regulator of systemic acquired resistance, are evaluated for health and environmental risks. These citrus lines exhibit strong HLB tolerance, with reduced disease symptoms, sustained fruit production, and no apparent negative phenotypic abnormalities. Comprehensive risk assessment reveals minimal exposure, health, or environmental risk. The AtNPR1 protein is: (1) barely detectable in fruit, (2) rapidly degraded in simulated gastrointestinal fluids, and (3) not similar to known allergens or toxins. Whole-genome sequencing identified the T-DNA insertion sites as heterozygous in either chromosome 1 or 6, with no disruptions in known fruit-producing genes. PCR markers were developed for rapid line identification. The selected lines are currently in a small field trial under high HLB pressure and continue to exhibit low visual HLB symptoms and positive horticultural traits. These findings support the initial requirements for regulatory approval of these transgenic citrus varieties, offering a promising strategy for sustainable citrus production.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"8 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yao Wang,Junbo Yang,Huiyu Hou,Luyang Song,Xu Cheng,Yong-Xin Liu
{"title":"Advancing Plant Microbiome Research Through Host DNA Depletion Techniques.","authors":"Yao Wang,Junbo Yang,Huiyu Hou,Luyang Song,Xu Cheng,Yong-Xin Liu","doi":"10.1111/pbi.70379","DOIUrl":"https://doi.org/10.1111/pbi.70379","url":null,"abstract":"Plants provide ecological habitats for diverse microorganisms, making accurate metagenomic sequencing essential for understanding the complex interactions that support plant growth, development and disease resistance. However, host DNA contamination poses a major challenge in plant microbiome studies, obscuring microbial genetic signatures and complicating the accurate analysis of microbial genomes. This review provides a comprehensive overview of current host DNA depletion strategies, including physical separation (e.g., filtration, gradient centrifugation), selective lysis and enzymatic treatments targeting plant cell walls. Advanced techniques such as targeted sequence capture with magnetic beads, methylation-based enrichment and nanopore selective sequencing offer additional options for host DNA removal. Despite these advances, current methods still face challenges in efficiency, specificity and applicability, emphasising the need for tailored strategies and the exploration of novel approaches for microbial enrichment. Innovations like CRISPR-Cas9 and chromatin immunoprecipitation-based host DNA depletion methods are proposed to provide novel directions for addressing current limitations. The development and refinement of host depletion techniques tailored to plant systems are crucial for enabling high-resolution, cost-effective metagenomic studies. These efforts promise to deepen our understanding of microbial diversity and functionality, ultimately accelerating microbiome-based innovations in crop improvement, sustainable agriculture and ecosystem resilience.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"79 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.","authors":"Tengfei Zhang,Jie Zang,Boming Yang,Qiuxia Wang,Jijun Yan,Peiyong Xin,Jinfang Chu,Huabang Chen,Zhaogui Zhang","doi":"10.1111/pbi.70400","DOIUrl":"https://doi.org/10.1111/pbi.70400","url":null,"abstract":"Vitamin B1 (VB1) plays a crucial role in sustaining plant health and enabling adaptive responses to environmental stress. The complex maize genome implies a sophisticated VB1 synthesis pathway, with the mechanisms by which VB1 benefits plants remaining elusive. Here, we identified two VB1 biosynthetic genes, THIAMINE REQUIRING 1 (ZmTH1) and its paralog THIAMINE MONOPHOSPHATE SYNTHASE 1 (ZmTMPS1), from a natural mutant pale leaf and depauperate growth 1 (pldg1). We elucidated their specific roles in regulating multiple thiamin diphosphate (TDP)-dependent metabolic pathways and their effects on plant growth and stress tolerance. ZmTH1 encodes a chloroplast-localised, bifunctional enzyme comprising phosphomethylpyrimidine kinase (HMPP-K) and thiamine monophosphate synthase (TMP-S) domains. Functional dissection revealed that these domains functioned synergistically, with disruption of one domain significantly attenuating the other, although both can function independently. A frameshift mutation in ZmTH1 (Zmth1) resulted in reduced biosynthesis of VB1, TMP and TDP. Consequently, the activity of TDP-dependent enzymes was impaired, disrupting multiple TDP-dependent metabolic pathways. Additionally, ZmTMPS1, localised to the cytosol and nucleus, exhibited limited TMP-S activity that partially compensated for ZmTH1 mutation in pldg1 but cannot fully restore VB1 levels. Overexpression of ZmTH1 or exogenous VB1 application enhanced maize seedling tolerance to cold and drought stresses by increasing TDP-dependent enzyme activity. These findings advance the understanding of VB1 metabolism in maize and provide genetic targets for improving stress resilience and crop performance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"154 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenxian Liu,Xianglong Zhao,Yanpeng Li,Qiang Zhou,Iain R Searle,Wengang Xie,Zhibiao Nan,Zhipeng Liu
{"title":"Genome-Wide Association Study Reveals the Genetic Architecture and Key Drought and Yield Related Genes in Common Vetch (Vicia sativa L.).","authors":"Wenxian Liu,Xianglong Zhao,Yanpeng Li,Qiang Zhou,Iain R Searle,Wengang Xie,Zhibiao Nan,Zhipeng Liu","doi":"10.1111/pbi.70407","DOIUrl":"https://doi.org/10.1111/pbi.70407","url":null,"abstract":"Common vetch (Vicia sativa L.) is one of the most economically important annual pasture legumes worldwide. Many factors affect the yield of common vetch; however, the genetic architecture and gene functions associated with common vetch yield have not been explored. On the basis of 115.53 million single-nucleotide polymorphisms (SNPs) and 18.55 million insertions-deletions (InDels) identified in 222 diverse common vetch accessions, we performed a comprehensive genome-wide association study (GWAS) on the 4-year results for six yield-related agronomic traits (water loss rate, dry weight, lodging index, stem thickness, absolute height and natural height). In total, 2864 SNPs and 481 InDels were respectively identified to be significantly associated with these six traits. Furthermore, a water loss rate-related candidate gene (Vs-pyruvate decarboxylase 2 [VsPDC2]) was functionally characterised and demonstrated to be a key regulator of the water loss rate in the heterologous species Arabidopsis thaliana. This study is the first to use GWAS to investigate the genetic architecture and key regulatory genes associated with drought tolerance and yield in common vetch, thereby providing valuable insights for common vetch breeding and future research.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uncovering Convergent Pattern Recognition Receptors Recognising Phytophthora Across Plant Lineages.","authors":"Yong Pei,Yaning Zhao,Hui Wang,Yining Guo,Xinyi Gu,Jingkun Lv,Zhenjie Guo,Yanjun Chen,Yingkai Ren,Yanrong Ren,Jianyu Yan,Yuke Wang,Peiyun Ji,Danyu Shen,Zhiyuan Yin,Daolong Dou","doi":"10.1111/pbi.70409","DOIUrl":"https://doi.org/10.1111/pbi.70409","url":null,"abstract":"Pattern recognition receptors (PRRs) are pivotal for plant immunity, yet their discovery in crops is hindered by lineage-specific divergence. We demonstrate that microbe-associated molecular patterns (MAMPs) often activate immunity through phylogenetically unrelated, convergently evolved PRRs across plant lineages. Using the Phytophthora-derived MAMP RLK6 as a prototype, we identified two leucine-rich repeat receptor-like proteins (LRR-RLPs), NbRKR1 and NbRKR2, that redundantly perceive RLK6 in the model plant Nicotiana benthamiana. Strikingly, soybean retained RLK6 responsiveness despite lacking NbRKR1/2 orthologs. By integrating AlphaFold3 structural prediction with functional screening in N. benthamiana receptor mutants, we uncovered GmRLP30 as the convergent RLK6 receptor in soybean. Phylogenetic analysis revealed RKR1/2 conservation in Solanaceae but their absence in Capsicum annuum, which encodes a truncated RKR1 variant incapable of activating RLK6 immunity. Critically, heterologous expression of NbRKR1 or GmRLP30 in pepper restored RLK6 perception, confirming functional equivalence. These results establish a direct receptor-mediated communication between pathogen and host surfaces, an ortholog-independent pipeline for rapid PRR mining across crops, and a foundation for engineering synthetic immune interfaces with durable disease resistance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"339 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolomic and Single‐Cell Transcriptomic Analyses Shed Light on Secondary Metabolite Profiling and Potential Developmental Dynamics of Glandular Trichomes in Artemisia argyi","authors":"Shuting Dong, Hongyu Chen, Sijie Sun, Miaoxian Guo, Chao Sun, Shilin Chen, Hongmei Luo","doi":"10.1111/pbi.70362","DOIUrl":"https://doi.org/10.1111/pbi.70362","url":null,"abstract":"Plant glandular trichomes (GTs) are characterised by their ability to synthesise and store abundant secondary metabolites of significant biological importance. The medicinal plant <jats:italic>Artemisia argyi</jats:italic> exhibits a dense covering of GTs, yet the precise metabolic compositions and the developmental molecular dynamics of <jats:italic>A. argyi</jats:italic> GTs remain insufficiently characterised. Using liquid chromatography–mass spectrometry (LC–MS) and gas chromatography–mass spectrometry (GC–MS), we identified 969 differentially accumulated metabolites (DAMs) between GTs and non‐glandular trichomes (NGTs) of <jats:italic>A. argyi</jats:italic>. GTs were significantly enriched in diverse metabolites, including terpenoids, flavonoids and fatty acyls, among which sesquiterpenoids were the most abundant terpenoid subclass in DAMs. To elucidate the molecular basis of GT secondary metabolite biosynthesis and development, we generated a single‐cell transcriptomic atlas of <jats:italic>A. argyi</jats:italic> leaves, annotating cell populations of mesophyll cells (MCs), epidermal cells (ECs), vascular cells (VCs), stomatal guard cells (GCs) and GTs. Pseudotime trajectory analysis uncovered the continuous developmental trajectory of GTs and identified several candidate transcription factors (TFs) potentially involved in GT development. Leveraging the single‐cell atlas, we constructed cell‐type‐specific co‐expression networks for sesquiterpene biosynthesis genes. Within the GT‐specific expression module, we identified β‐caryophyllene synthase AarTPS77, while the EC‐specific AarTPS52 was found to function as β‐farnesene synthase. Furthermore, we functionally characterised AarTPS95 and AarTPS96, which catalyse the formation of germacrene A and 12 additional sesquiterpenoids. These findings provide insights into the molecular basis of trichome development and secondary metabolite accumulation in <jats:italic>A. argyi</jats:italic>, laying the foundation for improving the quality of medicinal materials.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Rhus chinensis Genome Provides Insights Into Tannin, Flavonoid Biosynthesis, and Glandular Trichome Development","authors":"Zhaogeng Lu, Helin Zou, Jiawen Cui, Tongfei Wang, Lingyu Ma, Shixiong Ren, Yiwei Cao, Xi Zhang, Zixi Chen, Hongyan Bao, Ling Zhu, Yaning Cui, Ruili Li, Xiuyan Yang, Qikun Zhang, Zhili Wang, Wangxiang Zhang, Li Wang, Jinxing Lin, Biao Jin","doi":"10.1111/pbi.70392","DOIUrl":"https://doi.org/10.1111/pbi.70392","url":null,"abstract":"The “salt tree”, <jats:styled-content style=\"fixed-case\"><jats:italic>Rhus chinensis</jats:italic></jats:styled-content>, holds significant economic and medicinal value due to its ability to produce <jats:italic>Galla chinensis</jats:italic> (Chinese gall/gallnut), a plant‐derived medicinal material used in both traditional Chinese and modern medicine that is rich in tannins and flavonoids. It is also renowned for its remarkable stress tolerance. However, the genetic basis underlying its tannin and flavonoid biosynthesis and stress adaptation remains largely unexplored. Here, we assembled a chromosome‐level genome of <jats:styled-content style=\"fixed-case\"><jats:italic>R. chinensis</jats:italic></jats:styled-content> with a size of 357.62 Mb. A significant expansion of defence‐related genes, particularly those involved in chitin catabolism and flavonoid biosynthesis, explains the tree's extensive environmental adaptability. We identified key genes involved in tannin biosynthesis and hydrolysis, with <jats:italic>RcTA1</jats:italic> playing a central role in gallic acid accumulation, a precursor of hydrolyzable tannins. Notably, RcDIV1 promotes tannin hydrolysis by directly activating <jats:italic>RcTA1</jats:italic> transcription. Additionally, we uncovered that well‐developed multicellular glandular trichomes, regulated by RcGL2, along with an expanded array of transporters (e.g., ABCGs) and an enhanced ABA response, play critical roles in mediating salt tolerance. These factors collectively drive the production of salt‐like secretions, including phenolic and organic acids, which coat the fruit surface. Our study provides profound insights into the genetic mechanisms governing abundant tannin accumulation, flavonoid biosynthesis, glandular trichome development, and stress resilience, offering valuable genetic resources for improving the medicinal and ecological traits of this species.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"36 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haixia Zeng, Dandan Dou, Yang Yang, Yan Yan, Yawen Sun, Shuhan Yang, Wen Yao, Shifang Zhao, Mingle Wang, Zhixue Liu, Zhenzhen Ren, Huihui Su, Liru Cao, Lixia Ku, Xu Zheng, Chengwei Li, Yanhui Chen
{"title":"The ZmFKF1b‐ZmDi19‐5 Regulatory Module Coordinates Drought Tolerance and Flowering Time in Maize","authors":"Haixia Zeng, Dandan Dou, Yang Yang, Yan Yan, Yawen Sun, Shuhan Yang, Wen Yao, Shifang Zhao, Mingle Wang, Zhixue Liu, Zhenzhen Ren, Huihui Su, Liru Cao, Lixia Ku, Xu Zheng, Chengwei Li, Yanhui Chen","doi":"10.1111/pbi.70404","DOIUrl":"https://doi.org/10.1111/pbi.70404","url":null,"abstract":"Drought is a major environmental stress that inhibits plant growth and reduces crop yields. The <jats:italic>Di19</jats:italic> gene family is known to play a pivotal role in mediating plant responses to drought. However, the mechanisms by which Di19 proteins integrate drought response with developmental processes, particularly flowering time, remain largely unknown in maize. In this study, we reveal that <jats:italic>ZmDi19‐5</jats:italic> possesses a dual function in regulating both drought tolerance and flowering in maize. Overexpression of <jats:italic>ZmDi19‐5</jats:italic> not only enhanced drought tolerance but also delayed flowering time. Furthermore, we demonstrate that the ZmDi19‐5 and ZmFKF1b proteins interact both in vivo and in vitro. In contrast to the <jats:italic>zmfkf1b</jats:italic> mutants, plants overexpressing <jats:italic>ZmFKF1b</jats:italic> exhibited increased sensitivity to drought and accelerated flowering. Mechanistically, the interaction with ZmFKF1b attenuates the binding of ZmDi19‐5 to the promoter of its downstream targets, including the transcription factor <jats:italic>ZmHsf08</jats:italic> and the flowering inhibitor <jats:italic>ZmCOL3</jats:italic>, subsequently affecting their expression. In conclusion, our findings reveal that the ZmFKF1b‐ZmDi19‐5 module coordinates drought stress responses and flowering time in maize, providing a promising target for breeding drought‐resistant maize varieties with stable agronomic traits.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"25 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TaSPX3 Enhances Wheat Resistance to Leaf Rust by Antagonising TaDi19‐Mediated Repression of Pathogenesis‐Related Genes","authors":"Huimin Qian, Chuang Li, Yanan Lu, Xue Li, Jianping Zhang, Junyi Zhao, Keyan Wu, Yanyan Zhang, Kun Cheng, Daowen Wang, Pengyu Song, Na Liu, Wenming Zheng","doi":"10.1111/pbi.70402","DOIUrl":"https://doi.org/10.1111/pbi.70402","url":null,"abstract":"Wheat leaf rust, caused by <jats:italic>Puccinia triticina</jats:italic> (<jats:italic>Pt</jats:italic>), threatens global wheat production, with yield losses further exacerbated by the pathogen's evolving virulence. Although Syg1/Pho81/Xpr1 (SPX) domain‐containing proteins are known regulators of phosphate homeostasis, their involvement in plant–pathogen interactions remains largely unexplored. We demonstrated that <jats:italic>TaSPX3</jats:italic>, a wheat SPX family gene, is rapidly induced during early <jats:italic>Pt</jats:italic> infection and flg22 treatment. Genetic evidence indicates that <jats:italic>TaSPX3</jats:italic> is a positive regulator of rust resistance, with knockdown lines showing increased susceptibility and overexpression lines exhibiting enhanced resistance. Using yeast two‐hybrid screening, we identified TaDi19‐1D, a zinc finger transcription factor, as a direct TaSPX3 interactor. TaDi19‐1D functions as a negative immune regulator by suppressing the expression of pathogenesis‐related (PR) genes (<jats:italic>TaPR1</jats:italic>, <jats:italic>TaPR2</jats:italic>, <jats:italic>TaPR5</jats:italic>) through direct promoter binding. TaSPX3 counteracts this repression by physically interacting with TaDi19‐1D, thereby derepressing PR gene expression and boosting wheat resistance to <jats:italic>Pt</jats:italic>. Our findings revealed a novel TaSPX3–TaDi19 regulatory module that fine‐tunes <jats:italic>TaPRs</jats:italic> expression, providing mechanistic insights into pattern‐triggered immunity (PTI) and potential genetic targets for breeding durable broad‐spectrum disease‐resistant wheat varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"20 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}