Plant Biotechnology Journal最新文献

{"title":"Comparative genomics‐driven design of virus‐delivered short RNA inserts triggering robust gene silencing","authors":"Arcadio García, Verónica Aragonés, Silvia Gioiosa, Francisco J. Herraiz, Paloma Ortiz‐García, Jaime Prohens, José‐Antonio Daròs, Fabio Pasin","doi":"10.1111/pbi.70254","DOIUrl":"https://doi.org/10.1111/pbi.70254","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"21 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OrchardQuant-3D: combining drone and LiDAR to perform scalable 3D phenotyping for characterising key canopy and floral traits in fruit orchards.","authors":"Yunpeng Xia,Hanghang Li,Fanhang Zhang,Gang Sun,Kaijie Qi,Robert Jackson,Felipe Pinheiro,Xiaoman Liu,Yue Mu,Shaoling Zhang,Greg Deakin,E Charles Whitfield,Shutian Tao,Ji Zhou","doi":"10.1111/pbi.70229","DOIUrl":"https://doi.org/10.1111/pbi.70229","url":null,"abstract":"Orchard fruits such as pear and apple are important for ensuring global food security and agricultural economy as they not only provide essential nutrients, but also support biodiversity and ecosystem services. Breeders, growers and plant researchers constantly study desirable tree morphological features and floral characteristics to ensure fruit production and quality. Still, traditional orchard phenotyping is often laborious, limited in scale and prone-to-error, resulting in many attempts to develop reliable and scalable toolkits to address this challenge. Here, we present OrchardQuant-3D, an analytic pipeline for automating tree-level analysis of key canopy and floral traits for different types of fruit orchards. We first built a data fusion algorithm to register 3D point clouds collected by both drones (for colour signals) and Light Detection And Ranging (LiDAR, for precise spatial properties), reconstructing high-quality 3D orchard models at different growth stages. Then, we utilised precise global navigation satellite system signals to position trees in orchards with millimetre-level accuracy, enabling tree-level analysis of key canopy (e.g. crown volume and the number or branches) and floral traits (e.g. blossom clusters and volumes) using 3D computer vision, complex graph theory and feature engineering techniques. Equipped with the OrchardQuant-3D pipeline, we successfully measured varietal differences of four pear cultivars from a small pear orchard in Nanjing China, followed by a scale-up study that surveyed 3D tree morphologies, key floral and fruit traits from 1104 apple trees in an orchard in East Malling, United Kingdom. To the best of our knowledge, such a multi-source, comprehensive and expandable methodology has not yet been introduced to this important research domain. Hence, we believe that our work demonstrates a step change in our ability to conduct scalable 3D orchard phenotyping, which is highly valuable to advance orchard breeding, precise tree management and orchard research greatly to sustain fruit tree production in a rapidly changing climate.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PopulusPtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation","authors":"Dimiru Tadesse, Yuqiu Dai, Gen Li, Lin Yang, Yang Yang, Nidhi Dwivedi, Desigan Kumaran, Crysten E. Blaby‐Haas, Anna Lipzen, Kassandra Santiago, Kerrie Barry, Gary Coleman, Yiping Qi, Chang‐Jun Liu, Meng Xie","doi":"10.1111/pbi.70275","DOIUrl":"https://doi.org/10.1111/pbi.70275","url":null,"abstract":"The lack of a mechanistic understanding of the environmental plasticity of secondary cell wall (SCW) biosynthesis restricts large‐scale biomass and bioenergy production on marginal lands. Using <jats:italic>Populus</jats:italic> (poplar), a key bioenergy crop, we discovered that iron deprivation, a prevalent abiotic stress on marginal lands, stimulates SCW biosynthesis in stems. We identified the transcription factor PtrbHLH011 as a critical regulator underlying this response. Through integrated analyses involving phenotypic characterisation of <jats:italic>PtrbHLH011</jats:italic> knockout and overexpression plants, functional genomics and molecular investigations, we established that PtrbHLH011 functions as a central regulator of SCW biosynthesis, iron homeostasis and flavonoid biosynthesis by directly repressing essential genes in these pathways. Iron deprivation downregulates PtrbHLH011 expression, subsequently activating these biosynthetic pathways. Notably, cytosine base editing‐based knockout of <jats:italic>PtrbHLH011</jats:italic> significantly enhanced plant growth, yielding up to a 110% increase in stem diameter and a 300% increase in leaf iron content. These findings present a novel regulatory mechanism linking environmental iron availability to SCW biosynthesis and illustrate a practical strategy to improve biomass yield on iron‐deficient marginal lands. Furthermore, our mechanistic insights into PtrbHLH011 target recognition and regulation provide a valuable foundation for precise manipulation of gene regulatory networks, facilitating the development of high‐performance bioenergy crops adapted to marginal environments.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ABF5b‐HsfA2h/HsfC2a‐NCED2b/POD4/HSP26 module integrates multiple signaling pathway to modulate heat stress tolerance in wheat","authors":"Ji‐Tong Wei, Lei Zheng, Xiao‐Jun Ma, Tai‐Fei Yu, Xiang Gao, Ze‐Hao Hou, Yong‐Wei Liu, Xin‐You Cao, Jun Chen, Yong‐Bin Zhou, Ming Chen, Qi‐Yan Jiang, You‐Zhi Ma, Wei‐Jun Zheng, Zhao‐Shi Xu","doi":"10.1111/pbi.70164","DOIUrl":"https://doi.org/10.1111/pbi.70164","url":null,"abstract":"SummaryHeat stress caused by increasing global temperature has become a major factor limiting yield in wheat. Heat shock transcription factors (Hsfs), as the primary regulators in plant responses to heat stress, play essential roles in modulating both basal and acquired thermotolerance in plants. However, the underlying molecular mechanisms remain to be elucidated. By analysing the wheat transcriptome after subjecting wheat to heat treatments for different time intervals, we identified gene <jats:italic>TaHsfA2h</jats:italic> that showed a significant positive regulatory response to heat stress. Heat stress tolerance was enhanced by overexpression of <jats:italic>TaHsfA2h</jats:italic> and constrained by its RNA interference. RNA‐seq analysis demonstrated that the overexpression of <jats:italic>TaHsfA2h</jats:italic> significantly enhanced the expression levels of genes involved in ABA and ROS signalling pathways. Additionally, we identified <jats:italic>TaABF5b</jats:italic>, a critical regulatory factor in the ABA signalling pathway, as being capable of modulating the expression of <jats:italic>TaHsfA2h</jats:italic>. Notably, TaHsfA2h interacted with TaHsfC2a both <jats:italic>in vivo</jats:italic> and <jats:italic>in vitro</jats:italic>. Similarly, overexpression of <jats:italic>TaHsfC2a</jats:italic> significantly enhanced heat stress tolerance, whereas knockout dramatically reduced tolerance. The presence of TaHsfC2a significantly enhanced the regulatory activity of TaHsfA2h. TaHsfA2h and TaHsfC2a can co‐regulate the expression levels of heat stress tolerance‐related genes, including <jats:italic>TaNCED2B</jats:italic>, <jats:italic>TaPOD4</jats:italic> and <jats:italic>TaHSP26</jats:italic>, thereby enhancing wheat's tolerance to heat stress. Overall, our findings revealed a positive regulatory function of the ABF5b‐HsfA2h/HsfC2a‐NCED2b/POD4/HSP26 module on wheat heat stress tolerance. This discovery further expanded the functionality of a plant heat stress response model, providing a theoretical foundation for the development of heat‐tolerant wheat varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 14‐3‐3 Protein SlTFT1 Accelerates Tomato Fruit Ripening by Binding and Stabilising YFT1 in the Ethylene Signalling Pathway","authors":"Tengjian Wen, Lichun Cao, Lida Zhang, Lingxia Zhao","doi":"10.1111/pbi.70274","DOIUrl":"https://doi.org/10.1111/pbi.70274","url":null,"abstract":"Ethylene plays a pivotal role during the fruit ripening process in tomato (<jats:styled-content style=\"fixed-case\"><jats:italic>Solanum lycopersicum</jats:italic></jats:styled-content>). Previously, we have reported that the tomato EIN2‐like protein YELLOW‐FRUITED TOMATO1 (YFT1), a core component in the ethylene signal transduction pathway, exerts a critical regulatory function in the tomato fruit ripening process. However, the molecular mechanism of YFT1‐mediated ethylene signalling during tomato ripening still remains largely unknown. In this study, we performed yeast two‐hybrid screens of a cDNA expression library from tomato fruits and obtained a new YFT1‐interacting protein, SlTFT1 (TOMATO FOURTEEN‐THREE PROTEIN 1), and confirmed their interaction in vitro and in vivo. Further bimolecular fluorescence complementation (BiFC) assays indicated that SlTFT1 can specifically bind the canonical and non‐canonical on the carboxyl terminus of YFT1 (YFT1‐C). Triple response assays and ethylene responsive gene expression analysis demonstrated that SlTFT1 positively regulates the ethylene signalling pathway in a YFT1‐dependent manner. Phenotypical and biochemical analysis of SlTFT1 overexpression lines and loss‐of‐function mutants showed that <jats:italic>SlTFT1</jats:italic> significantly accelerated ethylene emission, chromoplast development, lycopene accumulation and fruit ripening rates; however, the deletion of <jats:italic>YFT1</jats:italic> and/or <jats:italic>SlTFT1</jats:italic> lesion generated the opposite results, suggesting that <jats:italic>SlTFT1</jats:italic> positively regulates tomato fruit ripening also in a YFT1‐dependent manner. Co‐expression and western blotting assays showed that SlTFT1 can efficiently prevent YFT1 from protein degradation mediated by an F‐box protein SlETP2‐like3, suggesting that SlTFT1 can stabilise YFT1 through their physical interaction. Collectively, our results reveal a genetic and molecular framework of the SlTFT1‐YFT1 complex, which modulates tomato fruit ripening by regulating ethylene signalling.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"91 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation Trajectory of Virus-Induced Tumour Cells in Rice Revealed by Single-Cell RNA Sequencing.","authors":"Nan Wu,Lu Gan,Qingqing Suo,Fei Yang,Wenwen Liu,Xifeng Wang,Huaibing Jin","doi":"10.1111/pbi.70267","DOIUrl":"https://doi.org/10.1111/pbi.70267","url":null,"abstract":"Many plant viruses trigger abnormal differentiation and development of host cells, causing distinct symptoms. Here, in a single-cell RNA sequence analysis, we find transcriptional heterogeneity between cells from rice leaf sheaths that are infected with rice black streaked dwarf virus (RBSDV) and from those that are virus-free. Using 106 973 cells, we construct a single-cell transcriptome atlas with 12 cell types and find that parenchyma and vascular parenchyma cells are the two major cell types affected by RBSDV. Notably, in RBSDV-infected plants, virus-induced tumours are observed to proliferate and show to differentiate from vascular parenchyma cells in a pseudotime analysis. During the differentiation of RBSDV-induced tumour cells, genes that involved in such processes as nucleosome assembly and chromatin remodeling are upregulated, and those involved in photosynthesis and energy metabolism are downregulated. A gene module of DGP1-GLK1-LHCBs that functions in regulating chloroplast development is repressed in the process of tumour cell formation. Moreover, a jasmonate-inducible pathogenesis-related gene, JiPR10, is expressed specifically in RBSDV-induced tumour cells and endows host resistance to RBSDV by inhibiting the proliferation and expansion of tumours. Our cell-type-specific atlas for plant responses to viral infection provides a valuable resource for further study of virus-induced abnormal development of host plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"37 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Ethylene‐Response Factor LlERF092 Coordinates With LlETO1 to Improve Thermotolerance by Activating LlMBF1c in Lily","authors":"Jun Xiang, Xue Gong, Qianqian Fang, Liping Ding, Yinyi Zhang, Sujuan Xu, Ting Li, Man He, Ze Wu, Nianjun Teng","doi":"10.1111/pbi.70269","DOIUrl":"https://doi.org/10.1111/pbi.70269","url":null,"abstract":"Multiprotein bridging factor 1c (MBF1c) has been shown to play a critical role in plant responses to heat stress. Previous studies have implicated MBF1c roles in ethylene‐mediated thermotolerance; however, the upstream regulatory mechanisms linking MBF1c to this process remain unclear. In this study, an ethylene‐response factor (ERF), LlERF092, was identified as a potential regulator of <jats:italic>LlMBF1c</jats:italic> through a yeast one‐hybrid screening assay. Further investigations revealed that LlERF092 directly bound to the promoter of <jats:italic>LlMBF1c</jats:italic> and activated its transcription. <jats:italic>LlERF092</jats:italic> was rapidly induced by heat stress, and its protein localised to the nucleus. Overexpression of <jats:italic>LlERF092</jats:italic> enhanced the thermotolerance of the transgenic lily plants. Furthermore, immunoprecipitation followed by mass spectrometry (IP‐MS) identified LlETO1 (ETHYLENE OVERPRODUCER 1) as an interacting partner of LlERF092. The expression of <jats:italic>LlETO1</jats:italic> was activated in response to transient heat stress, and the LlETO1‐LlERF092 interaction enhanced the transcriptional activity of LlERF092. Co‐overexpression of <jats:italic>LlERF092</jats:italic> and <jats:italic>LlETO1</jats:italic> enhanced thermotolerance more than the overexpression of either gene alone, while co‐silencing of <jats:italic>LlERF092</jats:italic> and <jats:italic>LlETO1</jats:italic> further reduced thermotolerance compared to silencing each gene individually. Additionally, heat stress promoted ethylene production in lily leaves, and exogenous application of ethephon enhanced thermotolerance. Ethephon treatment also elevated the expression of <jats:italic>LlERF092</jats:italic>, <jats:italic>LlETO1</jats:italic>, and <jats:italic>LlMBF1c</jats:italic>, while their expression was repressed by 1‐MCP under heat stress. In summary, these findings demonstrated that the LlERF092/LlETO1‐LlMBF1c transcriptional cascade mediated ethylene‐dependent thermotolerance in lily under heat stress conditions. This study provides new insights into the molecular mechanisms underlying plant heat stress responses and highlights the role of ethylene signalling in thermotolerance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PagHB7/PagABF4–PagEPFL9 Module Regulates Stomatal Density and Drought Tolerance in Poplar","authors":"Yufei Xia, Ruihua Guo, Te Lu, Shenxiu Jiang, Kairan You, Xinli Xia, Kang Du, Xiangyang Kang","doi":"10.1111/pbi.70273","DOIUrl":"https://doi.org/10.1111/pbi.70273","url":null,"abstract":"<jats:italic>Epidermal patterning factor‐like 9</jats:italic> (<jats:italic>EPFL9</jats:italic>) influences stomatal density and growth in poplar. There have been no reports on <jats:italic>homeobox 7</jats:italic> (<jats:italic>HB7</jats:italic>) and <jats:italic>ABRE binding factor 4</jats:italic> (<jats:italic>ABF4</jats:italic>) regulating stomatal density or drought tolerance by targeting <jats:italic>EPFL</jats:italic>9 in poplar. This study revealed that <jats:italic>EPFL9</jats:italic> was specifically localised in guard cells in leaves and responded to drought stress. By constructing CRISPR/Cas9‐mediated <jats:italic>PagEPFL9</jats:italic> gene‐edited lines, we found that <jats:italic>epfl9</jats:italic> mutant plants showed significantly reduced stomatal density, inhibited growth and enhanced drought resistance. However, <jats:italic>PagEPFL9</jats:italic> overexpression increased its drought stress sensitivity by increasing the stomatal density. <jats:italic>PagHB7</jats:italic> was demonstrated to be an upstream regulator of <jats:italic>PagEPFL9</jats:italic> by yeast one‐hybrid screening library experiments, yeast one‐hybrid experiments, electrophoretic mobility shift assay and dual luciferase reporter gene assay experiments. Yeast two‐hybrid, bimolecular fluorescence complementation, split luciferase complementation assays, GST pull‐down, electrophoretic mobility shift assay and dual luciferase reporter gene assay experiments further demonstrated that <jats:italic>PagHB7</jats:italic> interacted with <jats:italic>PagABF4</jats:italic> and that <jats:italic>PagABF4</jats:italic> enhanced the inhibitory effect of <jats:italic>PagHB7</jats:italic> on <jats:italic>PagEPFL9</jats:italic>. Knockout plants of <jats:italic>PagHB7</jats:italic>, a negative regulator of <jats:italic>PagEPFL9</jats:italic>, had a significantly increased stomatal density and reduced drought tolerance. Poplars overexpressing <jats:italic>PagABF4</jats:italic> showed similar phenotypes to poplars knocking out <jats:italic>PagEPFL9</jats:italic>, with stomatal density significantly lower than that of WT, which may result in greater drought tolerance. Our study demonstrates that <jats:italic>PagHB7</jats:italic> and <jats:italic>PagABF4</jats:italic> interact with each other and regulate stomatal density by targeting <jats:italic>PagEPFL9</jats:italic>, thereby affecting drought resistance in poplar. This study provides new genetic resources for molecular design breeding of plant growth and drought tolerance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"76 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome‐scale haplotype‐resolved genome assembly of the autotetraploid alfalfa cultivar Bolivia","authors":"Hongkui Zhang, Lan Zhou, Hong Zhao, Jiayan Liang, Yongle Liu, Chen Wang, Sijie Sun, Lizhen Song, Yu'e Zhang, Youfa Cheng, Yongbiao Xue","doi":"10.1111/pbi.70259","DOIUrl":"https://doi.org/10.1111/pbi.70259","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APPi: A Multiscale Qualitative–Quantitative Insecticide‐Likeness Evaluation Platform and Application","authors":"Jia‐lin Cui, Qi He, Bin‐yan Jin, Xin‐peng Sun, Hua Li, Yue Wei, Xiao‐ming Zhang, Li Zhang","doi":"10.1111/pbi.70271","DOIUrl":"https://doi.org/10.1111/pbi.70271","url":null,"abstract":"According to the Food and Agriculture Organization of the United Nations (FAO), pests reduce global crop production by 14% annually. The growing challenge of pest resistance, coupled with the relatively low success rates of pesticides, has prompted researchers to shift their attention towards the accurate evaluation of insecticide lead. In contrast to in vitro methods of structural similarity or target affinity, the ‘insecticide‐likeness’ approach emphasises the in vivo biological effects of compounds, thereby constructing precise and comprehensive evaluation rules. In the present study, a multi‐scale qualitative‐quantitative insecticide‐likeness evaluation platform, Agrochem Predictive Platform for Insecticide‐likeness (APPi), was developed. An APPi rule was proposed for qualitative evaluation (ClogP ≤ 7, ARB ≤ 18, HBA ≤ 7, HBD ≤ 2, PFI ≤ 8 and ROB ≤ 10). A quantitative insecticide‐likeness evaluation model, the APPi model, was developed based on a multi‐classifier integrated machine learning framework (PUMV). The APPi model demonstrated excellent performance on the train and external test sets. Crucially, on the independent external test set, it achieved an accuracy of 85%, which represents a significant improvement over existing models. Furthermore, we developed the FragScore Visualiser tool to identify critical insecticidal fragments of compounds. The APPi platform provides precise guidance for virtual screening and structure optimisation of lead compounds in the early stage of insecticides discovery. The platform is available free of charge at <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://pesticides.cau.edu.cn/APPi\">http://pesticides.cau.edu.cn/APPi</jats:ext-link>.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}