{"title":"OrchardQuant-3D: combining drone and LiDAR to perform scalable 3D phenotyping for characterising key canopy and floral traits in fruit orchards.","authors":"Yunpeng Xia,Hanghang Li,Fanhang Zhang,Gang Sun,Kaijie Qi,Robert Jackson,Felipe Pinheiro,Xiaoman Liu,Yue Mu,Shaoling Zhang,Greg Deakin,E Charles Whitfield,Shutian Tao,Ji Zhou","doi":"10.1111/pbi.70229","DOIUrl":"https://doi.org/10.1111/pbi.70229","url":null,"abstract":"Orchard fruits such as pear and apple are important for ensuring global food security and agricultural economy as they not only provide essential nutrients, but also support biodiversity and ecosystem services. Breeders, growers and plant researchers constantly study desirable tree morphological features and floral characteristics to ensure fruit production and quality. Still, traditional orchard phenotyping is often laborious, limited in scale and prone-to-error, resulting in many attempts to develop reliable and scalable toolkits to address this challenge. Here, we present OrchardQuant-3D, an analytic pipeline for automating tree-level analysis of key canopy and floral traits for different types of fruit orchards. We first built a data fusion algorithm to register 3D point clouds collected by both drones (for colour signals) and Light Detection And Ranging (LiDAR, for precise spatial properties), reconstructing high-quality 3D orchard models at different growth stages. Then, we utilised precise global navigation satellite system signals to position trees in orchards with millimetre-level accuracy, enabling tree-level analysis of key canopy (e.g. crown volume and the number or branches) and floral traits (e.g. blossom clusters and volumes) using 3D computer vision, complex graph theory and feature engineering techniques. Equipped with the OrchardQuant-3D pipeline, we successfully measured varietal differences of four pear cultivars from a small pear orchard in Nanjing China, followed by a scale-up study that surveyed 3D tree morphologies, key floral and fruit traits from 1104 apple trees in an orchard in East Malling, United Kingdom. To the best of our knowledge, such a multi-source, comprehensive and expandable methodology has not yet been introduced to this important research domain. Hence, we believe that our work demonstrates a step change in our ability to conduct scalable 3D orchard phenotyping, which is highly valuable to advance orchard breeding, precise tree management and orchard research greatly to sustain fruit tree production in a rapidly changing climate.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dimiru Tadesse, Yuqiu Dai, Gen Li, Lin Yang, Yang Yang, Nidhi Dwivedi, Desigan Kumaran, Crysten E. Blaby‐Haas, Anna Lipzen, Kassandra Santiago, Kerrie Barry, Gary Coleman, Yiping Qi, Chang‐Jun Liu, Meng Xie
{"title":"PopulusPtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation","authors":"Dimiru Tadesse, Yuqiu Dai, Gen Li, Lin Yang, Yang Yang, Nidhi Dwivedi, Desigan Kumaran, Crysten E. Blaby‐Haas, Anna Lipzen, Kassandra Santiago, Kerrie Barry, Gary Coleman, Yiping Qi, Chang‐Jun Liu, Meng Xie","doi":"10.1111/pbi.70275","DOIUrl":"https://doi.org/10.1111/pbi.70275","url":null,"abstract":"The lack of a mechanistic understanding of the environmental plasticity of secondary cell wall (SCW) biosynthesis restricts large‐scale biomass and bioenergy production on marginal lands. Using <jats:italic>Populus</jats:italic> (poplar), a key bioenergy crop, we discovered that iron deprivation, a prevalent abiotic stress on marginal lands, stimulates SCW biosynthesis in stems. We identified the transcription factor PtrbHLH011 as a critical regulator underlying this response. Through integrated analyses involving phenotypic characterisation of <jats:italic>PtrbHLH011</jats:italic> knockout and overexpression plants, functional genomics and molecular investigations, we established that PtrbHLH011 functions as a central regulator of SCW biosynthesis, iron homeostasis and flavonoid biosynthesis by directly repressing essential genes in these pathways. Iron deprivation downregulates PtrbHLH011 expression, subsequently activating these biosynthetic pathways. Notably, cytosine base editing‐based knockout of <jats:italic>PtrbHLH011</jats:italic> significantly enhanced plant growth, yielding up to a 110% increase in stem diameter and a 300% increase in leaf iron content. These findings present a novel regulatory mechanism linking environmental iron availability to SCW biosynthesis and illustrate a practical strategy to improve biomass yield on iron‐deficient marginal lands. Furthermore, our mechanistic insights into PtrbHLH011 target recognition and regulation provide a valuable foundation for precise manipulation of gene regulatory networks, facilitating the development of high‐performance bioenergy crops adapted to marginal environments.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ji‐Tong Wei, Lei Zheng, Xiao‐Jun Ma, Tai‐Fei Yu, Xiang Gao, Ze‐Hao Hou, Yong‐Wei Liu, Xin‐You Cao, Jun Chen, Yong‐Bin Zhou, Ming Chen, Qi‐Yan Jiang, You‐Zhi Ma, Wei‐Jun Zheng, Zhao‐Shi Xu
{"title":"An ABF5b‐HsfA2h/HsfC2a‐NCED2b/POD4/HSP26 module integrates multiple signaling pathway to modulate heat stress tolerance in wheat","authors":"Ji‐Tong Wei, Lei Zheng, Xiao‐Jun Ma, Tai‐Fei Yu, Xiang Gao, Ze‐Hao Hou, Yong‐Wei Liu, Xin‐You Cao, Jun Chen, Yong‐Bin Zhou, Ming Chen, Qi‐Yan Jiang, You‐Zhi Ma, Wei‐Jun Zheng, Zhao‐Shi Xu","doi":"10.1111/pbi.70164","DOIUrl":"https://doi.org/10.1111/pbi.70164","url":null,"abstract":"SummaryHeat stress caused by increasing global temperature has become a major factor limiting yield in wheat. Heat shock transcription factors (Hsfs), as the primary regulators in plant responses to heat stress, play essential roles in modulating both basal and acquired thermotolerance in plants. However, the underlying molecular mechanisms remain to be elucidated. By analysing the wheat transcriptome after subjecting wheat to heat treatments for different time intervals, we identified gene <jats:italic>TaHsfA2h</jats:italic> that showed a significant positive regulatory response to heat stress. Heat stress tolerance was enhanced by overexpression of <jats:italic>TaHsfA2h</jats:italic> and constrained by its RNA interference. RNA‐seq analysis demonstrated that the overexpression of <jats:italic>TaHsfA2h</jats:italic> significantly enhanced the expression levels of genes involved in ABA and ROS signalling pathways. Additionally, we identified <jats:italic>TaABF5b</jats:italic>, a critical regulatory factor in the ABA signalling pathway, as being capable of modulating the expression of <jats:italic>TaHsfA2h</jats:italic>. Notably, TaHsfA2h interacted with TaHsfC2a both <jats:italic>in vivo</jats:italic> and <jats:italic>in vitro</jats:italic>. Similarly, overexpression of <jats:italic>TaHsfC2a</jats:italic> significantly enhanced heat stress tolerance, whereas knockout dramatically reduced tolerance. The presence of TaHsfC2a significantly enhanced the regulatory activity of TaHsfA2h. TaHsfA2h and TaHsfC2a can co‐regulate the expression levels of heat stress tolerance‐related genes, including <jats:italic>TaNCED2B</jats:italic>, <jats:italic>TaPOD4</jats:italic> and <jats:italic>TaHSP26</jats:italic>, thereby enhancing wheat's tolerance to heat stress. Overall, our findings revealed a positive regulatory function of the ABF5b‐HsfA2h/HsfC2a‐NCED2b/POD4/HSP26 module on wheat heat stress tolerance. This discovery further expanded the functionality of a plant heat stress response model, providing a theoretical foundation for the development of heat‐tolerant wheat varieties.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"14 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nan Wu,Lu Gan,Qingqing Suo,Fei Yang,Wenwen Liu,Xifeng Wang,Huaibing Jin
{"title":"Differentiation Trajectory of Virus-Induced Tumour Cells in Rice Revealed by Single-Cell RNA Sequencing.","authors":"Nan Wu,Lu Gan,Qingqing Suo,Fei Yang,Wenwen Liu,Xifeng Wang,Huaibing Jin","doi":"10.1111/pbi.70267","DOIUrl":"https://doi.org/10.1111/pbi.70267","url":null,"abstract":"Many plant viruses trigger abnormal differentiation and development of host cells, causing distinct symptoms. Here, in a single-cell RNA sequence analysis, we find transcriptional heterogeneity between cells from rice leaf sheaths that are infected with rice black streaked dwarf virus (RBSDV) and from those that are virus-free. Using 106 973 cells, we construct a single-cell transcriptome atlas with 12 cell types and find that parenchyma and vascular parenchyma cells are the two major cell types affected by RBSDV. Notably, in RBSDV-infected plants, virus-induced tumours are observed to proliferate and show to differentiate from vascular parenchyma cells in a pseudotime analysis. During the differentiation of RBSDV-induced tumour cells, genes that involved in such processes as nucleosome assembly and chromatin remodeling are upregulated, and those involved in photosynthesis and energy metabolism are downregulated. A gene module of DGP1-GLK1-LHCBs that functions in regulating chloroplast development is repressed in the process of tumour cell formation. Moreover, a jasmonate-inducible pathogenesis-related gene, JiPR10, is expressed specifically in RBSDV-induced tumour cells and endows host resistance to RBSDV by inhibiting the proliferation and expansion of tumours. Our cell-type-specific atlas for plant responses to viral infection provides a valuable resource for further study of virus-induced abnormal development of host plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"37 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Xiang, Xue Gong, Qianqian Fang, Liping Ding, Yinyi Zhang, Sujuan Xu, Ting Li, Man He, Ze Wu, Nianjun Teng
{"title":"An Ethylene‐Response Factor LlERF092 Coordinates With LlETO1 to Improve Thermotolerance by Activating LlMBF1c in Lily","authors":"Jun Xiang, Xue Gong, Qianqian Fang, Liping Ding, Yinyi Zhang, Sujuan Xu, Ting Li, Man He, Ze Wu, Nianjun Teng","doi":"10.1111/pbi.70269","DOIUrl":"https://doi.org/10.1111/pbi.70269","url":null,"abstract":"Multiprotein bridging factor 1c (MBF1c) has been shown to play a critical role in plant responses to heat stress. Previous studies have implicated MBF1c roles in ethylene‐mediated thermotolerance; however, the upstream regulatory mechanisms linking MBF1c to this process remain unclear. In this study, an ethylene‐response factor (ERF), LlERF092, was identified as a potential regulator of <jats:italic>LlMBF1c</jats:italic> through a yeast one‐hybrid screening assay. Further investigations revealed that LlERF092 directly bound to the promoter of <jats:italic>LlMBF1c</jats:italic> and activated its transcription. <jats:italic>LlERF092</jats:italic> was rapidly induced by heat stress, and its protein localised to the nucleus. Overexpression of <jats:italic>LlERF092</jats:italic> enhanced the thermotolerance of the transgenic lily plants. Furthermore, immunoprecipitation followed by mass spectrometry (IP‐MS) identified LlETO1 (ETHYLENE OVERPRODUCER 1) as an interacting partner of LlERF092. The expression of <jats:italic>LlETO1</jats:italic> was activated in response to transient heat stress, and the LlETO1‐LlERF092 interaction enhanced the transcriptional activity of LlERF092. Co‐overexpression of <jats:italic>LlERF092</jats:italic> and <jats:italic>LlETO1</jats:italic> enhanced thermotolerance more than the overexpression of either gene alone, while co‐silencing of <jats:italic>LlERF092</jats:italic> and <jats:italic>LlETO1</jats:italic> further reduced thermotolerance compared to silencing each gene individually. Additionally, heat stress promoted ethylene production in lily leaves, and exogenous application of ethephon enhanced thermotolerance. Ethephon treatment also elevated the expression of <jats:italic>LlERF092</jats:italic>, <jats:italic>LlETO1</jats:italic>, and <jats:italic>LlMBF1c</jats:italic>, while their expression was repressed by 1‐MCP under heat stress. In summary, these findings demonstrated that the LlERF092/LlETO1‐LlMBF1c transcriptional cascade mediated ethylene‐dependent thermotolerance in lily under heat stress conditions. This study provides new insights into the molecular mechanisms underlying plant heat stress responses and highlights the role of ethylene signalling in thermotolerance.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"APPi: A Multiscale Qualitative–Quantitative Insecticide‐Likeness Evaluation Platform and Application","authors":"Jia‐lin Cui, Qi He, Bin‐yan Jin, Xin‐peng Sun, Hua Li, Yue Wei, Xiao‐ming Zhang, Li Zhang","doi":"10.1111/pbi.70271","DOIUrl":"https://doi.org/10.1111/pbi.70271","url":null,"abstract":"According to the Food and Agriculture Organization of the United Nations (FAO), pests reduce global crop production by 14% annually. The growing challenge of pest resistance, coupled with the relatively low success rates of pesticides, has prompted researchers to shift their attention towards the accurate evaluation of insecticide lead. In contrast to in vitro methods of structural similarity or target affinity, the ‘insecticide‐likeness’ approach emphasises the in vivo biological effects of compounds, thereby constructing precise and comprehensive evaluation rules. In the present study, a multi‐scale qualitative‐quantitative insecticide‐likeness evaluation platform, Agrochem Predictive Platform for Insecticide‐likeness (APPi), was developed. An APPi rule was proposed for qualitative evaluation (ClogP ≤ 7, ARB ≤ 18, HBA ≤ 7, HBD ≤ 2, PFI ≤ 8 and ROB ≤ 10). A quantitative insecticide‐likeness evaluation model, the APPi model, was developed based on a multi‐classifier integrated machine learning framework (PUMV). The APPi model demonstrated excellent performance on the train and external test sets. Crucially, on the independent external test set, it achieved an accuracy of 85%, which represents a significant improvement over existing models. Furthermore, we developed the FragScore Visualiser tool to identify critical insecticidal fragments of compounds. The APPi platform provides precise guidance for virtual screening and structure optimisation of lead compounds in the early stage of insecticides discovery. The platform is available free of charge at <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://pesticides.cau.edu.cn/APPi\">http://pesticides.cau.edu.cn/APPi</jats:ext-link>.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaping Tang, Xinyan Shen, Xuan Deng, Yingda Song, Yuhong Zhou, Yongen Lu, Feng Li, Bo Ouyang
{"title":"Establishment of an efficient Agrobacterium‐mediated transformation system for chilli pepper and its application in genome editing","authors":"Yaping Tang, Xinyan Shen, Xuan Deng, Yingda Song, Yuhong Zhou, Yongen Lu, Feng Li, Bo Ouyang","doi":"10.1111/pbi.70216","DOIUrl":"https://doi.org/10.1111/pbi.70216","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"52 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mi‐Young Kim, Andy Cano Tran, Ju Kim, Humblenoble Stembridge Ayuk, Adam Sparrow, Lorenzo Bossi, Megan Brown, Emil Joseph Vergara, Kathrin Göritzer, Elisabetta Groppelli, Tae‐Ho Kwon, Julian K. C. Ma, Yong‐Suk Jang, Rajko Reljic
{"title":"Structural Design and Immunogenicity of a Novel Self‐Adjuvanting Mucosal Vaccine Candidate for SARS‐CoV‐2 Expressed in Plants","authors":"Mi‐Young Kim, Andy Cano Tran, Ju Kim, Humblenoble Stembridge Ayuk, Adam Sparrow, Lorenzo Bossi, Megan Brown, Emil Joseph Vergara, Kathrin Göritzer, Elisabetta Groppelli, Tae‐Ho Kwon, Julian K. C. Ma, Yong‐Suk Jang, Rajko Reljic","doi":"10.1111/pbi.70278","DOIUrl":"https://doi.org/10.1111/pbi.70278","url":null,"abstract":"Mucosal vaccination for COVID‐19 to boost preexisting though insufficient systemic and local/mucosal immunity remains an attractive prospect but there are currently no licensed mucosal vaccines against this infection. Here, using a plant expression system, we developed a novel mucosal vaccine platform for respiratory viruses and demonstrated its application in the context of SARS‐CoV‐2 infection. In addition to the antigen itself, the PCF (Platform CTB‐Fc) vaccine candidate incorporates two molecular adjuvants, the IgG‐Fc antibody fragment and the nontoxic cholera toxin B subunit (CTB), with the first targeting the vaccine to IgG receptors on antigen‐presenting cells, and the second providing local adjuvanticity by targeting cellular gangliosides in the mucosae. We demonstrated that this vaccine candidate is highly immunogenic in mice, inducing virus‐neutralising systemic and mucosal antibodies as well as tissue resident memory T cells in the lungs. We also demonstrated that SRBD‐PCF is recognised by immune cells from exposed or vaccinated individuals, and that circulating antibodies also bind to the antigen within the vaccine, forming immune complexes (IC). Finally, with a view of respiratory delivery, we demonstrated that the vaccine can be aerosolised without loss of material or biological activity, and that it is noncytotoxic and nonhaemolytic to human cells. Furthermore, we demonstrate that the plant expression system represents a suitable platform to produce these complex, multifunctional macromolecules capable of simultaneously binding to multiple targets. Our data strongly support the case for a safe, self‐adjuvanting mucosal COVID‐19 vaccine development, as means to boosting both systemic and mucosal immunity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"52 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana‐Maria Pantazica, Alexander Hammel, Iuliana Caras, Irina Ionescu, Catalin Tucureanu, Adrian Onu, Maria Murace, Jihong Liu Clarke, Crina Stavaru, Norica Branza‐Nichita, Ralph Bock
{"title":"Red Alga Porphyridium Supports High‐Yield Production of a Functional Chimeric Hepatitis B Surface Antigen With Strong Cellular and Humoral Immunogenicity","authors":"Ana‐Maria Pantazica, Alexander Hammel, Iuliana Caras, Irina Ionescu, Catalin Tucureanu, Adrian Onu, Maria Murace, Jihong Liu Clarke, Crina Stavaru, Norica Branza‐Nichita, Ralph Bock","doi":"10.1111/pbi.70270","DOIUrl":"https://doi.org/10.1111/pbi.70270","url":null,"abstract":"Microalgae represent promising production factories for the light‐driven, cost‐effective production of recombinant proteins. The red microalga <jats:styled-content style=\"fixed-case\"><jats:italic>Porphyridium purpureum</jats:italic></jats:styled-content> displays particularly favourable transgene expression properties due to the episomal maintenance of transformation vectors at high copy numbers in the nucleus. In this work, we explored the potential of <jats:styled-content style=\"fixed-case\"><jats:italic>Porphyridium purpureum</jats:italic></jats:styled-content> to synthesise a candidate vaccine against Hepatitis B virus (HBV). We show high‐yield expression of an HBV chimeric surface antigen and efficient assembly of virus‐like particles (VLPs) in algal cells. We established a purification protocol for the VLPs and conducted vaccination studies in experimental animals. The results demonstrate that the alga‐produced HBV antigen elicits superior humoral and cellular immune responses compared to a commercial HBV vaccine produced in yeast. The antigen triggers virus‐neutralising antibodies against different HBV variants, including vaccine‐escape mutations that evade the immune response to current vaccines in humans. Our work establishes <jats:italic>Porphyridium</jats:italic> as a highly promising production platform for vaccines and other proteinaceous biopharmaceuticals.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}