Plant Biotechnology Journal最新文献

{"title":"Cell-Specific Expression and Cellular Compartmental Regulation in Camptothecin Biosynthesis","authors":"Xiaolong Hao,&nbsp;Yinkai Yang,&nbsp;Tiantian Chen,&nbsp;Xiaoxuan Fan,&nbsp;Yiqing Peng,&nbsp;Qingyan Ruan,&nbsp;Qin Zhou,&nbsp;Fanghao Liu,&nbsp;Jiayi He,&nbsp;Yongpeng Li,&nbsp;Yue Feng,&nbsp;Jiyan Qi,&nbsp;Guoyin Kai","doi":"10.1111/pbi.70687","DOIUrl":"10.1111/pbi.70687","url":null,"abstract":"<p>Plant secondary metabolites such as monoterpenoid indole alkaloids (MIAs) show tightly regulated biosynthesis and accumulation in specific organelles of distinct cell types. However, the cell-specific expression patterns of anticancer MIA camptothecin biosynthetic genes in <i>Ophiorrhiza pumila</i> and the regulatory mechanisms of camptothecin biosynthesis via cellular compartmentalisation remain poorly understood. Here, single-cell RNA sequencing of <i>O. pumila</i> leaves generated 9181 cells, classified into four types using marker gene annotation and in situ hybridisation. Gene expression profiling combined with pseudotime analyses revealed that camptothecin biosynthetic genes are preferentially expressed in epidermal and young cells. Notably, the genes encoding camptothecin key enzymes, strictosidine synthase (STR) and tryptophan decarboxylase (TDC), have expanded within the <i>O. pumila</i> <i>STR-TDC</i> gene cluster, supporting cell-specific specialisation. Concurrently, this gene cluster has evolved a novel functional gene, <i>OpAVT1</i>, which annotated as a vacuolar transporter, mediates the transport of tryptophan from vacuole to the cytosol, thereby regulating camptothecin and other MIAs accumulation. Collectively, these findings establish a cellular-level framework for camptothecin biosynthesis and regulation.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5114-5130"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148025400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel AP2/ERF Transcription Factor Controls Trichome Initiation and Fruit Development in Cucumber","authors":"Haifan Wen,&nbsp;Hui Du,&nbsp;Siqi Wang,&nbsp;Yiqun Weng,&nbsp;Juan Liu,&nbsp;Huanle He,&nbsp;Run Cai,&nbsp;Jian Pan,&nbsp;Junsong Pan","doi":"10.1111/pbi.70676","DOIUrl":"10.1111/pbi.70676","url":null,"abstract":"<p>Trichomes, the hair-like outgrowths of the plant epidermis, are critical for defence, reproduction and development. However, the underlying molecular mechanisms governing their initiation remain elusive. Here, we identified CsGL2, an AP2/ERF transcription factor, as a key integrator controlling trichome initiation and fruit development in cucumber. Genetic and molecular studies indicate that CsGL2 activates <i>CsGL1</i>/<i>Mict</i>, subsequently triggering the morphogenesis of multicellular trichomes. Beyond epidermal fate, CsGL2 upregulates the tubercle regulator <i>Tu</i> together with the cytokinin rate-limiting enzyme gene <i>IPT1</i>, thereby initiating fruit tubercles through cytokinin-linked pathways. Notably, we found that ovule-specific CsGL2 activates the parthenocarpy gene <i>CsNPF1</i>, and <i>CsGL2</i> overexpression markedly increases the frequency of seedless fruit set. Thus, our findings establish a switch-based mechanism for progressive cell-fate specification in multicellular epidermal organs. This reveals how a single transcription factor integrates transcriptional and hormonal pathways to coordinate distinct developmental programmes.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5077-5093"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB Factors: Hubs of Plant Stress and Hormone Crosstalk","authors":"Aye Aye Htun,&nbsp;Ya Wang,&nbsp;Jiahao Liu,&nbsp;Zhiqiang Guo,&nbsp;Yi Zheng,&nbsp;Wenjie Wang,&nbsp;Shuting Ma,&nbsp;Aolin Jia,&nbsp;Yan Ren,&nbsp;Feng Chen","doi":"10.1111/pbi.70694","DOIUrl":"10.1111/pbi.70694","url":null,"abstract":"<p>MYB transcription factors function as main regulatory hubs that integrate environmental signals with multi-hormonal pathways to synchronize plant growth, metabolism and stress responses. This review delineates the regulatory roles of MYB in the signalling pathway of salicylic acid, jasmonic acid, ethylene and abscisic acid signalling. These are responsible for regulating hormone biosynthesis and facilitating interaction between biotic and abiotic stress responses. The post-translational regulation of MYB activity by kinase cascades and the direct regulation of secondary metabolic pathways, such as the biosynthesis of phenylpropanol and lignin, by MYBs through the MYB-bHLH-WD40 (MBW) complex were investigated. MYB transcription factors located at the intersection of hormone signalling, metabolic regulation, and developmental regulation play a unique role in coordinating plant adaptation from the cellular level to the whole plant.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5201-5223"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatially Resolved ABA Signalling Reveals Dual Role in Tomato Yield","authors":"Fu-An Wen,&nbsp;Fu-Dong Mai,&nbsp;Ji-Bin Xiao,&nbsp;Hong Zhang,&nbsp;Xuan Zhou,&nbsp;Xin-Yu Yang,&nbsp;Yelena Sterlin,&nbsp;David Karpovsky,&nbsp;Ke-Ting Li,&nbsp;Juan Wang,&nbsp;Jia Liu,&nbsp;Shi-Hao Su,&nbsp;Assaf Mosquna,&nbsp;Yu-Fei Sun","doi":"10.1111/pbi.70700","DOIUrl":"10.1111/pbi.70700","url":null,"abstract":"<p>Abscisic acid (ABA) is a central hormonal regulator of plant adaptation to stress and developmental progression. However, its contribution to agronomic traits such as yield remains unclear due to the pleiotropic nature of ABA signalling. To disentangle distinct physiological functions of ABA, we employed a receptor-specific gain-of-function strategy in tomato, activating downstream signalling independently of endogenous hormone levels in an ABA-deficient background. This approach revealed two separable ABA-responsive modules that contribute to yield formation: one enhancing drought resilience through improved stomatal regulation, and another restoring reproductive success by promoting pollen viability and anther development. These physiological responses, governed by spatially distinct sites of ABA action, partially mitigated the yield penalties associated with ABA deficiency, although neither alone was sufficient to fully restore productivity. Our findings establish a dual functional framework by which ABA signalling coordinates water conservation with reproductive fitness, providing mechanistic insight into how hormone signalling integrates stress adaptation with crop yield. This modular understanding lays the foundation for targeted manipulation of ABA pathways to improve agricultural resilience under climate stress.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5224-5235"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staurosporine Targets Mitochondrial Regulator VdAtuA3 to Disrupt Mitochondrial Homeostasis to Control Verticillium Wilt","authors":"Ruo-Cheng Sheng,&nbsp;Huan Li,&nbsp;Jun Wang,&nbsp;Li-Chao Wang,&nbsp;Hui-Zi Liu,&nbsp;Xiao-Feng Dai,&nbsp;Steven J. Klosterman,&nbsp;Jie-Yin Chen,&nbsp;Krishna V. Subbarao,&nbsp;Feng-Mao Chen,&nbsp;Dan-Dan Zhang","doi":"10.1111/pbi.70690","DOIUrl":"10.1111/pbi.70690","url":null,"abstract":"<p>Mitochondria serve as cellular powerhouses that generate ATP via electron transport chain complexes and orchestrate metabolism-apoptosis cross-talk, yet genes maintaining mitochondrial homeostasis remain underexplored as antifungal targets. In this study, a previously uncharacterised protein VdAtuA3 was identified as a novel interactor with VdNuo1 (NADH: ubiquinone oxidoreductase 24-kDa subunit), defining a regulatory axis for the mitochondrial respiratory chain in <i>Verticillium dahliae</i>. Notably, VdNuo1 is highly conserved across plants, animals, and humans, consistent with its essential role in respiration, whereas VdAtuA3 homologues in non-fungal organisms share very low sequence similarity. Moreover, Y2H assays confirmed no interaction between Nuo1 and AtuA homologues from above organisms, indicating that this regulatory axis is specific to <i>V. dahliae</i>. The pathogen virulence factor VdAtuA3 and VdNuo1 co-regulate oxidative phosphorylation and superoxide detoxification, thereby promoting mitochondrial homeostasis. Staurosporine (STS), a natural microbial product, targets VdAtuA3, inhibiting its function and reducing the interaction between VdAtuA3 and VdNuo1, inducing mitochondrial dysfunction in <i>V. dahliae</i>, and suppressing its growth effectively controls Verticillium wilt. STS shows high efficacy with minimal off-target toxicity and can be safely applied to cotton, zebrafish, and human cells at fungistatic doses. Its broad-spectrum activity against multiple filamentous fungi, consistent with its high binding affinity to AtuA homologues from various filamentous fungi as demonstrated by molecular docking and functional complementation assays, further supports its potential as a promising antifungal agent. Our study provides a proof-of-concept for targeting pathogen-specific virulence factors that regulate mitochondrial homeostasis as a novel strategy to manage fungal plant pathogens.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5131-5151"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Transcriptional Switch for Recombinant Protein Expression in Seed Plastids","authors":"Malihe Mirzaee,&nbsp;Evelyn V. Wachowski,&nbsp;Injy H. Elgamal,&nbsp;Pal Maliga","doi":"10.1111/pbi.70696","DOIUrl":"10.1111/pbi.70696","url":null,"abstract":"&lt;p&gt;The RNA polymerase of bacteriophage T7 (T7 RNAP) is highly selective for its promoters and is widely used for the expression of recombinant proteins in &lt;i&gt;Escherichia coli&lt;/i&gt; (Studier et al. &lt;span&gt;1990&lt;/span&gt;). In plants, the plastid genes are transcribed by two RNA polymerases: the plastid-encoded multisubunit plastid RNA polymerase (PEP) and the phage-type, nuclear-encoded single subunit RNA polymerase (NEP). NEP does not recognize the T7 RNAP gene 10 promoter (PT7) and leader containing a ribosome binding site in vivo (McBride et al. &lt;span&gt;1994&lt;/span&gt;) or in chloroplast extracts (Liere and Maliga &lt;span&gt;1999&lt;/span&gt;), and T7 RNAP does not recognize plastid NEP promoters (Liere and Maliga &lt;span&gt;1999&lt;/span&gt;), making the T7 RNAP suitable for application as an orthogonal transcription system in plastids. Here, we employ the T7 RNAP and the cognate PT7 promoters for seed-specific expression of plastid transgenes activated by a seed-specific promoter.&lt;/p&gt;&lt;p&gt;Expression of recombinant proteins in plastids has the advantages of high-level protein accumulation, expression of multiple genes in operons, and maternal inheritance for biocontainment (Maliga &lt;span&gt;2021&lt;/span&gt;). However, plastid transgenes are usually expressed in leaf chloroplasts, which are perishable and require lyophilization of leaf or purification of recombinant proteins (Twyman et al. &lt;span&gt;2003&lt;/span&gt;). In contrast, seeds are natural storage organs that accumulate and protect the proteins during extended storage. Recently, we showed that seed plastids can serve as a platform for recombinant protein expression using a post-transcriptional activation system based on seed-specific expression of a plastid-targeted PPR10 RNA-binding protein, PPR10&lt;sup&gt;GG&lt;/sup&gt;, which binds its cognate site, BS&lt;sup&gt;GG&lt;/sup&gt;, located upstream of plastid transgenes (Mirzaee et al. &lt;span&gt;2024&lt;/span&gt;). Because this system is regulated post-transcriptionally, the mRNA is present even in the absence of PPR10&lt;sup&gt;GG&lt;/sup&gt;, for example, in leaves, resulting in recombinant protein accumulation in non-target tissues. Background levels of recombinant proteins in a posttranscriptionally regulated system are high, ~2% of total soluble cellular protein (TSP) in leaves. Transcriptional control restricts plastid mRNA production to the target tissue, avoiding unintended recombinant protein accumulation in non-target tissues. T7 RNAP has previously been used to selectively transcribe plastid transgenes driven by the cognate PT7 promoter, by expressing it from the nucleus and importing it into leaf chloroplasts (McBride et al. &lt;span&gt;1994&lt;/span&gt;; Lossl et al. &lt;span&gt;2005&lt;/span&gt;; Latif et al. &lt;span&gt;2022&lt;/span&gt;). Here, we extend the utilization of the orthogonal T7 RNAP/PT7 regulatory system for seed-specific expression of plastid transgenes.&lt;/p&gt;&lt;p&gt;For seed-specific expression of transgenes in plastids, we designed a two-component system. The first component is a nuclear transgene encoding the T7 RNAP translationally fused to a ","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5111-5113"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148025416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable Domestication: CRISPR, Pan-Genomics and System Level Engineering for Next-Generation Crops.","authors":"Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei","doi":"10.1111/pbi.70749","DOIUrl":"10.1111/pbi.70749","url":null,"abstract":"<p><p>Global agriculture is increasingly challenged by climate instability, genetic erosion, emerging pathogens and rising food demands, exposing the limitations of conventional breeding and traditional domestication strategies. Recent advances in CRISPR-based genome editing, pangenomic, synthetic biology, artificial intelligence (AI)-assisted breeding and predictive phenomics are transforming de novo domestication from a slow evolutionary process into a programmable framework for rational crop redesign. This review synthesises recent advances in programmable de novo domestication and highlights how crop wild relatives and underutilised germplasm can be harnessed to develop resilient, climate-adaptive and sustainable crop systems. The integration of multiplex genome editing, pan-genomic variation discovery, AI-driven genomic prediction and predictive breeding enables precise engineering of key domestication traits governing plant architecture, yield potential, stress resilience and nutritional quality. Furthermore, we propose a trajectory-based framework for programmable domestication comprising Adaptive Rescue, Agronomic Refinement and Novel Chassis Engineering, which illustrates distinct evolutionary pathways, engineering complexity and crop redesign objectives. We also examine the major system level challenges that constrain programmable domestication, including cryptic genetic variation, epistasis, gene regulatory network complexity, genotype phenotype predictability, biodiversity conservation and regulatory considerations. Collectively, programmable domestication represents a transformative shift from conventional crop improvement towards system-level engineering of next-generation crops, providing a strategic foundation for enhancing global food security, agricultural sustainability and environmental resilience in the face of accelerating climate change.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13507823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Dual-Function of CtrNAC019-CtrNPF2.1 Module in Salt Tolerance and Nitrogen Use Efficiency Via Enhancing Vacuolar Chloride Sequestration and Nitrate Efflux in Citrus trifoliata","authors":"Zeqi Zhao,&nbsp;Chengwei Yang,&nbsp;Xiangming Shang,&nbsp;Mengdi Li,&nbsp;Xiaoyang Su,&nbsp;Ji-Hong Liu,&nbsp;Chunlong Li","doi":"10.1111/pbi.70686","DOIUrl":"10.1111/pbi.70686","url":null,"abstract":"<p>Salt stress and nitrogen utilisation efficiency (NUE) represent critical constraints affecting worldwide crop productivity. While nitrate transporter proteins (NPFs) have been implicated in saline chloride (Cl⁻) ion transport, the mechanistic linkage between chloride stress and NUE remains poorly understood. Through comparative transcriptomic profiling of <i>Citrus trifoliata</i>, we identified <i>CtrNPF2.1</i> as a dual-responsive gene significantly upregulated in root tissues under both chloride salt and high nitrate conditions. The CtrNPF2.1 protein is localized to the vacuole membrane and highly expressed in root cortical cells. Transport functional characterisation combined with transgenic phenotypic analyses established that CtrNPF2.1 mediates dual transport mechanisms: facilitating vacuolar Cl⁻ compartmentalisation while enabling nitrate efflux. This coordinated process enhances cellular ion detoxification and promotes nitrate redistribution for optimal root development under saline conditions. Molecular investigations further uncovered that CtrNAC019 transcriptionally regulates <i>CtrNPF2.1</i> expression through direct binding to its promoter, forming a chloride/nitrate-responsive regulatory module. Notably, physiological validation reveals coordinated responses in which nitrate supplementation mitigates chloride toxicity while chloride availability enhances nitrogen utilisation in trifoliate orange, which may be explained by the CtrNAC019-CtrNPF2.1 expression regulation module. These results provide new insights into vacuolar chloride sequestration and nitrate efflux by CtrNAC019-CtrNPF2.1 under saline and high-nitrate conditions, offering a promising strategy for breeding salt-resilient and resource-efficient crops.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5059-5076"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147961678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting the Molecular Roadmap for Sugar Crops: Genome Reading, Trait Writing and Variety Redesigning","authors":"Peilin Wang,&nbsp;Qibin Wu,&nbsp;Wenzhi Wang,&nbsp;Prakash Lakshmanan,&nbsp;Yangrui Li,&nbsp;Khushi Muhammad,&nbsp;Yuguang Wang,&nbsp;Youxiong Que","doi":"10.1111/pbi.70683","DOIUrl":"10.1111/pbi.70683","url":null,"abstract":"<p>Sugar crops, including but not limited to sugarcane, sugar beet, sweet sorghum and stevia, are major sources of sugar production in the world. However, conventional breeding approaches, limited by long breeding cycles, low efficiency and restricted capacity to improve complex traits in sugar crops, are increasingly insufficient to address the challenges posed by climate change and the demands of sustainable agriculture. This review systematically summarizes recent advances in biotechnology and molecular breeding that have transformed sugar crop improvement. Recently, high-throughput sequencing technologies have generated extensive multi-omics resources. Concurrently, numerous functional genes and genetic elements with substantial breeding potential have been identified and cloned, offering precise targets for the key agronomic traits in sugar crops. Marker-assisted selection has been successfully implemented to enhance disease resistance, while genomic selection has demonstrated well for the evaluation and selection of complex quantitative traits. Importantly, genetic transformation systems have enabled precise manipulation of target genes and facilitated the creation of novel germplasm. In the future, the integration of multi-omics data, artificial intelligence, high-throughput phenotyping and precision genome editing into an intelligent breeding framework will be essential for achieving breeding by design and developing climate-adaptive and smart cultivars. Ultimately, these technological innovations will expand the role of sugar crops beyond traditional sugar production, positioning them as a central platform for sustainable biomanufacturing and providing critical support for global sugar security, energy transition and the development of the bioeconomy.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5013-5040"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147924895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR–Cas9 Mediated TcNPR3 Mutagenesis”","authors":"","doi":"10.1111/pbi.70722","DOIUrl":"10.1111/pbi.70722","url":null,"abstract":"<p>Guiltinan, M.J., Landherr, L., Maximova, S.N., DelVecchio, D., Sebastian, A. and Albert, I. (2026) Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR–Cas9 Mediated TcNPR3 Mutagenesis. Plant Biotechnology Journal 24: 442–454. https://doi.org/10.1111/pbi.70365</p><p>In the originally published article, the line designated GM-249 was identified as the cacao genotype Scavina-6 (Sca-6) and was used as a non-edited reference in the transcriptome analysis and as the pollen parent in one of the reported crosses. Whole-genome sequencing of the experimental genotypes has since established that GM-249 is the cacao clone ICS-1, not Scavina-6. The misidentification originated as a labeling error on the source plant and was detected only once genome-scale data became available; the corrected identity has been confirmed by whole-genome sequence comparison.</p><p>The correction changes the genotype label of GM-249 from “Scavina-6 (Sca-6)” to “ICS-1” in Table 1, the relevant figures and the supplementary materials, and in the description of the cross in which GM-249 (ICS-1) served as the pollen parent. A corrected Table 1 accompanies this correction.</p><p>This reassignment does not alter the study's principal conclusions. The most strongly supported and reproducible differential-expression signal derives from comparisons among lines in the PSU-Scavina-6 background, which do not involve GM-249 and are therefore unaffected. The corrected identity indicates that comparisons involving GM-249 span a more genetically divergent background than originally stated, which is consistent with, and reinforces, the paper's caution that genetic-background heterogeneity contributes substantially to the observed transcriptional variation. No quantitative result, figure, or conclusion other than the GM-249 genotype label requires revision.</p><p>We apologize for this error.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 9","pages":"5236-5238"},"PeriodicalIF":12.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70722","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148428813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}