Plant Biotechnology Journal最新文献

{"title":"Flexible substrate-based mass spectrometry platform for in situ non-destructive molecular imaging of living plants.","authors":"Guanhua Zhang, Han Zheng, Xiao Wang, Shuxin Han, Wei Liu, Chenglong Sun, Qiongzheng Hu, Chunxia Ma","doi":"10.1111/pbi.14482","DOIUrl":"10.1111/pbi.14482","url":null,"abstract":"<p><p>Monitoring and localizing molecules on living plants is critical for understanding their growth, development and disease. However, current techniques for molecular imaging of living plants often lack spatial information or require tedious pre-labelling. Here, we proposed a novel molecular imaging platform that combines sliver nanowire-doped Ti₃C₂ MXene (Ag NWs@MXene) flexible film substrate with laser desorption/ionization mass spectrometry imaging (AMF-LDI-MSI) to study the spatial distribution of biomolecules on the surface of living plants. This platform overcomes the MSI challenges posed by difficult-to-slice plant tissues (e.g., tough or water-rich roots and fragile flowers) and enables precisely transfer and visualize the molecule. Comparisons of the measurement results to those from matrix-assisted LDI-MSI (MALDI-MSI) technology demonstrate the accuracy and reliability of the platform. Biocompatibility evaluations indicated that the platform without observable adverse effects on the health of living plants. The distribution of growth and disease-associated signalling molecules, such as choline, organic acids and carbohydrates, can be in situ non-destructively detected on the surfaces of living plants, which is important for tracking the health of plants and their diseased areas. AMF-LDI-MSI platform can serve as a promising tool for label-free, in situ and non-destructive monitoring of functional biomolecules and plant growth from a spatial perspective.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"97-111"},"PeriodicalIF":10.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetraspanin 5 orchestrates resilience to salt stress through the regulation of ion and reactive oxygen species homeostasis in rice.","authors":"Balaji Mani, Inderjit Kaur, Yashika Dhingra, Vidisha Saxena, G K Krishna, Rahul Kumar, Viswanathan Chinnusamy, Manu Agarwal, Surekha Katiyar-Agarwal","doi":"10.1111/pbi.14476","DOIUrl":"10.1111/pbi.14476","url":null,"abstract":"<p><p>Tetraspanins (TETs) are integral membrane proteins, characterized by four transmembrane domains and a unique signature motif in their large extracellular loop. They form dynamic supramolecular complexes called tetraspanin-enriched microdomains (TEMs), through interactions with partner proteins. In plants, TETs are involved in development, reproduction and immune responses, but their role in defining abiotic stress responses is largely underexplored. We focused on OsTET5, which is differentially expressed under various abiotic stresses and localizes to both plasma membrane and endoplasmic reticulum. Using overexpression and underexpression transgenic lines we demonstrate that OsTET5 contributes to salinity and drought stress tolerance in rice. OsTET5 can interact with itself in yeast, suggesting homomer formation. Immunoblotting of native PAGE of microsomal fraction enriched from OsTET5-Myc transgenic rice lines revealed multimeric complexes containing OsTET5, suggesting the potential formation of TEM complexes. Transcriptome analysis, coupled with quantitative PCR-based validation, of OsTET5-altered transgenic lines unveiled the differential expression patterns of several stress-responsive genes, as well as those coding for transporters under salt stress. Notably, OsTET5 plays a crucial role in maintaining the ionic equilibrium during salinity stress, particularly by preserving an elevated potassium-to-sodium (K⁺/Na⁺) ratio. OsTET5 also regulates reactive oxygen species homeostasis, primarily by modulating the gene expression and activities of antioxidant pathway enzymes and proline accumulation. Our comprehensive investigation underscores the multifaceted role of OsTET5 in rice, accentuating its significance in developmental processes and abiotic stress tolerance. These findings open new avenues for potential strategies aimed at enhancing stress resilience and making valuable contributions to global food security.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"51-71"},"PeriodicalIF":10.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.","authors":"Elena Garcia-Perez, Marta Vazquez-Vilar, Rosa Lozano-Duran, Diego Orzaez","doi":"10.1111/pbi.14485","DOIUrl":"10.1111/pbi.14485","url":null,"abstract":"<p><p>The growing demand for sustainable platforms for biomolecule manufacturing has fuelled the development of plant-based production systems. Agroinfiltration, the current industry standard, offers several advantages but faces limitations for large-scale production due to high operational costs and batch-to-batch variability. Alternatively, here, we describe the CuBe system, a novel bean yellow dwarf virus (BeYDV)-derived conditional replicative expression platform stably transformed in Nicotiana benthamiana and activated by copper sulphate (CuSO₄), an inexpensive and widely used agricultural input. The CuBe system utilizes a synthetic circuit of four genetic modules integrated into the plant genome: (i) a replicative vector harbouring the gene of interest (GOI) flanked by cis-acting elements for geminiviral replication and novelly arranged to enable transgene transcription exclusively upon formation of the circular replicon, (ii) copper-inducible Rep/RepA proteins essential for replicon formation, (iii) the yeast-derived CUP2-Gal4 copper-responsive transcriptional activator for Rep/RepA expression, and (iv) a copper-inducible Flp recombinase to minimize basal Rep/RepA expression. CuSO₄ application triggers the activation of the system, leading to the formation of extrachromosomal replicons, expression of the GOI, and accumulation of the desired recombinant protein. We demonstrate the functionality of the CuBe system in N. benthamiana plants expressing high levels of eGFP and an anti-SARS-CoV-2 antibody upon copper treatment. Notably, the system is functional in post-harvest applications, a strategy with high potential impact for large-scale biomanufacturing. This work presents the CuBe system as a promising alternative to agroinfiltration for cost-effective and scalable production of recombinant proteins in plants.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"141-155"},"PeriodicalIF":10.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance.","authors":"Shiwei Ma, Shichang Xu, Huan Tao, Yunxia Huang, Changqing Feng, Guanpeng Huang, Shoukai Lin, Yiqiong Sun, Xuan Chen, Manegdebwaoga Arthur Fabrice Kabore, Samuel Tareke Woldegiorgis, Yufang Ai, Lina Zhang, Wei Liu, Huaqin He","doi":"10.1111/pbi.14494","DOIUrl":"10.1111/pbi.14494","url":null,"abstract":"<p><p>It is urgent to mine novel blast-resistant genes in rice and develop new rice varieties with pyramiding blast-resistant genes. In this study, a new blast-resistant gene, OsBRW1, was screened from a set of rice near-isogenic lines (NILs) with different blast-resistant ability. Under the infection of Magnaporthe oryzae (M. oryzae), OsBRW1 in the resistant NIL Pi-4b was highly induced than that in the susceptible NIL Pi-1 and their parent line CO39, and the blast-resistant ability of OsBRW1 was further confirmed by using CRISPR/Cas9 knockout and over-expression methods. The protein encoded by OsBRW1 was a typical NBS-LRR with NB-ARC domain and localized in both cytoplasm and nucleus, and the transient expression of OsBRW1 was capable of triggering hypersensitive response in tobacco leaves. Protein interaction experiments showed that OsBRW1 protein directly interacted with OsSRFP1. At the early infection stage of M. oryzae, OsBRW1 gene induced OsSRFP1 to highly expression level and accumulated H₂O₂, up-regulated the defence responsive signalling transduction genes and the pathogenesis-related genes and increased JA and SA content in the resistant NIL Pi-4b. By contrary, lower content of endogenous JA and SA in osbrw1 mutants was found at the same stage. After that, OsSRFP1 was down-regulated to constitution abundance to balance the growth of the resistant NIL Pi-4b. In summary, OsBRW1 solicited OsSRFP1 to resist the infection of blast fungus in rice by inducing the synergism of induced systemic resistance (ISR) and system acquired resistance (SAR) and to balance the growth of rice plants.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":"250-267"},"PeriodicalIF":10.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of nucleus‐encoded trans‐acting factors allows orthogonal fine‐tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii","authors":"Pawel Mateusz Mordaka, Kitty Clouston, Aleix Gorchs‐Rovira, Catherine Sutherland, Daniel Qingyang Zhang, Katrin Geisler, Payam Mehrshahi, Alison Gail Smith","doi":"10.1111/pbi.14557","DOIUrl":"https://doi.org/10.1111/pbi.14557","url":null,"abstract":"SummaryThe green microalga <jats:italic>Chlamydomonas reinhardtii</jats:italic> is a promising host organism for the production of valuable compounds. Engineering the <jats:italic>Chlamydomonas</jats:italic> chloroplast genome offers several advantages over the nuclear genome, including targeted gene insertion, lack of silencing mechanisms, potentially higher protein production due to multiple genome copies and natural substrate abundance for metabolic engineering. Tuneable expression systems can be used to minimize competition between heterologous production and host cell viability. However, complex gene regulation and a lack of tight regulatory elements make this a challenge in the <jats:italic>Chlamydomonas</jats:italic> chloroplast. In this work, we develop two synthetic tuneable systems to control the expression of genes on the chloroplast genome, taking advantage of the properties of the vitamin B<jats:sub>12</jats:sub>‐responsive <jats:italic>METE</jats:italic> promoter and a modified thiamine (vitamin B<jats:sub>1</jats:sub>) riboswitch, along with nucleus‐encoded chloroplast‐targeted regulatory proteins NAC2 and MRL1. We demonstrate the capacity of these systems for robust, fine‐tuned control of several chloroplast transgenes, by addition of nanomolar levels of vitamins. The two systems have been combined in a single strain engineered to avoid effects on photosynthesis and are orthogonal to each other. They were then used to manipulate the production of an industrially relevant diterpenoid, casbene, by introducing and tuning expression of the coding sequence for casbene synthase, as well as regulating the metabolite flux towards casbene precursors, highlighting the utility of these systems for informing metabolic engineering approaches.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"90 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel transcription factor OsMYB73 affects grain size and chalkiness by regulating endosperm storage substances' accumulation‐mediated auxin biosynthesis signalling pathway in rice","authors":"Song Liu, Jiamin Wu, Amos Musyoki Mawia, Xiangjin Wei, Ruijie Cao, Guiai Jiao, Yawen Wu, Jian Zhang, Lihong Xie, Zhonghua Sheng, Shikai Hu, Sanfeng Li, Yusong Lv, Feifei Lu, Yujuan Chen, Sajid Fiaz, Javaria Tabassum, Zhimin Du, Fangyuan Gao, Guangjun Ren, Gaoneng Shao, Peisong Hu, Shaoqing Tang","doi":"10.1111/pbi.14558","DOIUrl":"https://doi.org/10.1111/pbi.14558","url":null,"abstract":"SummaryEnhanced grain yield and quality traits are everlasting breeding goals. It is therefore of great significance to uncover more genetic resources associated with these two important agronomic traits. Plant MYB family transcription factors play important regulatory roles in diverse biological processes. However, studies on genetic functions of MYB in rice yield and quality are rarely to be reported. Here, we investigated a nucleus‐localized transcription factor <jats:italic>OsMYB73</jats:italic> which is preferentially expressed in the early developing pericarp and endosperm. We generated targeted mutagenesis of <jats:italic>OsMYB73</jats:italic> in rice, and the mutants had longer grains with obvious white‐belly chalky endosperm appearance phenotype. The mutants displayed various changes in starch physicochemical characteristics and lipid components. Transcriptome sequencing analysis showed that <jats:italic>OsMYB73</jats:italic> was chiefly involved in cell wall development and starch metabolism. <jats:italic>OsMYB73</jats:italic> mutation affects the expression of genes related to grain size, starch and lipid biosynthesis and auxin biosynthesis. Moreover, inactivation of <jats:italic>OsMYB73</jats:italic> triggers broad changes in secondary metabolites. We speculate that rice <jats:italic>OsMYB73</jats:italic> and <jats:italic>OsNF‐YB1</jats:italic> play synergistic pivotal role in simultaneously as transcription activators to regulate grain filling and storage compounds accumulation to affect endosperm development and grain chalkiness through binding <jats:italic>OsISA2</jats:italic>, <jats:italic>OsLTPL36</jats:italic> and <jats:italic>OsYUC11</jats:italic>. The study provides important germplasm resources and theoretical basis for genetic improvement of rice yield and quality. In addition, we enriches the potential biological functions of rice MYB family transcription factors.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"62 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of Fc-fusion protein structures to enhance efficacy of cancer vaccine in plant expression system","authors":"Sohee Lim, Hyun Joo Chung, Yoo Jin Oh, Peter Hinterdorfer, Soon Chul Myung, Young-Jin Seo, Kisung Ko","doi":"10.1111/pbi.14552","DOIUrl":"https://doi.org/10.1111/pbi.14552","url":null,"abstract":"Epithelial cell adhesion molecule (EpCAM) fused to IgG, IgA and IgM Fc domains was expressed to create IgG, IgA and IgM-like structures as anti-cancer vaccines in <i>Nicotiana tabacum</i>. High-mannose glycan structures were generated by adding a C-terminal endoplasmic reticulum (ER) retention motif (KDEL) to the Fc domain (FcK) to produce EpCAM-Fc and EpCAM-FcK proteins in transgenic plants via <i>Agrobacterium</i>-mediated transformation. Cross-fertilization of EpCAM-Fc (FcK) transgenic plants with Joining chain (J-chain, J and JK) transgenic plants led to stable expression of large quaternary EpCAM-IgA Fc (EpCAM-A) and IgM-like (EpCAM-M) proteins. Immunoblotting, SDS–PAGE and ELISA analyses demonstrated that proteins with KDEL had higher expression levels and binding activity to anti-EpCAM IgGs. IgM showed the strongest binding among the fusion proteins, followed by IgA and IgG. Sera from BALB/c mice immunized with these vaccines produced anti-EpCAM IgGs. Flow cytometry indicated that the EpCAM-Fc fusion proteins significantly activated CD8⁺ cytotoxic T cells, CD4⁺ helper T cells and B cells, particularly with EpCAM-FcK^P and EpCAM-Fc^P (FcK^P) × J^P (JK^P). The induced anti-EpCAM IgGs captured human prostate cancer PC-3 and colorectal cancer SW620 cells. Sera from immunized mice inhibited cancer cell proliferation, migration and invasion; down-regulated proliferation markers (PCNA, Ki-67) and epithelial–mesenchymal transition markers (Vimentin); and up-regulated E-cadherin. These findings suggest that <i>N. tabacum</i> can produce effective vaccine candidates to induce anti-cancer immune responses.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"127 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Editor‐in‐Chief","authors":"Johnathan Napier","doi":"10.1111/pbi.14560","DOIUrl":"https://doi.org/10.1111/pbi.14560","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"25 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel geminivirus‐derived 3′ flanking sequence of terminator mediates the gene expression enhancement","authors":"Yi Zhang, Yibo Xian, Heng Yang, Xuangang Yang, Tianli Yu, Sai Liu, Minting Liang, Xianzhi Jiang, Shulin Deng","doi":"10.1111/pbi.14561","DOIUrl":"https://doi.org/10.1111/pbi.14561","url":null,"abstract":"SummaryExploring the new elements to re‐design the expression cassette is crucial in synthetic biology. Viruses are one of the most important sources for exploring gene expression elements. In this study, we found that the DNA sequence of the SBG51 deltasatellite from the <jats:italic>Sweet potato leaf curl virus</jats:italic> (SPLCV) greatly enhanced the gene expression when flanked downstream of the terminator. The SBG51 sequence increased transient <jats:italic>GFP</jats:italic> gene expression in <jats:italic>Nicotiana benthamiana</jats:italic> leaves by up to ~6 times and ~10 times compared to the gene expression controlled by the UBQ10 promoter and 35S promoter alone, respectively. The increased <jats:italic>GFP</jats:italic> gene expression level contributed to the continuous accumulation of GFP protein and GFP fluorescence until 8 days post‐inoculation (dpi). The SBG51 sequence also enhanced the gene expression in the transgenic Arabidopsis plants and maintained the spatio‐temporal pattern of the <jats:italic>FLOWERING LOCUS T</jats:italic> (<jats:italic>FT</jats:italic>) and <jats:italic>TOO MANY MOUTHS</jats:italic> (<jats:italic>TMM</jats:italic>) promoters. We identified a 123 bp of AT‐rich sequence containing seven “ATAAA” or “TTAAA” elements from the SBG51 DNA, which had the gene expression enhancement effect. Furthermore, the artificial synthetic sequences containing tandem repeated “ATAAA” or “TTAAA” elements were sufficient to increase the gene expression but did not alter the polyadenylation of mRNA, similar to the function of matrix attachment regions (MAR). Additionally, the compact artificial synthetic sequence also had an effect on yeast when the expression cassette was integrated into the genome. We conclude that the geminivirus deltasatellite‐derived sequence and the “ATAAA”/“TTAAA” elements are powerful tools for enhancing gene expression.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome of root celery and population genomic analysis reveal the complex breeding history of celery","authors":"Enhui Lai, Sumin Guo, Pan Wu, Minghao Qu, Xiaofen Yu, Chenlu Hao, Shan Li, Haixu Peng, Yating Yi, Miao Zhou, Guodong Fu, Xingnuo Li, Huan Liu, Yi Zheng, Xin Wang, Zhangjun Fei, Lei Gao","doi":"10.1111/pbi.14551","DOIUrl":"https://doi.org/10.1111/pbi.14551","url":null,"abstract":"SummaryCelery (<jats:italic>Apium graveolens</jats:italic> L.) is an important vegetable crop in the Apiaceae family. It comprises three botanical varieties: common celery with solid and succulent petioles, celeriac or root celery with enlarged and fleshy hypocotyls and smallage or leaf celery with slender, leafy and usually hollow petioles. Here we present a chromosome‐level genome assembly of a celeriac cultivar and a comprehensive genome variation map constructed through resequencing of 177 representative celery accessions. Phylogenetic analysis revealed that smallage from the Mediterranean region represented the most ancient type of cultivated celery. Following initial domestication in this region, artificial selection has primarily aimed at enlarging the hypocotyl, resulting in celeriac, and at solidifying the petiole, leading to common celery. Selective sweep analysis and genome‐wide association study identified several genes associated with hypocotyl expansion and revealed that the hollow/solid petiole trait directly correlated with the presence/absence of a NAC gene. Our study elucidates the complex breeding history of celery and provides valuable genomic resources and molecular insights for future celery improvement and conservation efforts.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"76 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}