Plant Biotechnology Journal最新文献_第10页

A module with multiple transcription factors positively regulates powdery mildew resistance in grapevine. 一个多转录因子模块正调控葡萄对白粉病的抗性。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70196

Xiuming Zhang,Qihan Zhang,Yanxun Zhu,Yichu Zhang,Xiaoxiao Yan,Chunlei Guo,Min Gao,Zhi Li,Yijie Zhao,Hua Lu,Xiping Wang

{"title":"A module with multiple transcription factors positively regulates powdery mildew resistance in grapevine.","authors":"Xiuming Zhang,Qihan Zhang,Yanxun Zhu,Yichu Zhang,Xiaoxiao Yan,Chunlei Guo,Min Gao,Zhi Li,Yijie Zhao,Hua Lu,Xiping Wang","doi":"10.1111/pbi.70196","DOIUrl":"https://doi.org/10.1111/pbi.70196","url":null,"abstract":"Erysiphe necator, the causal agent of powdery mildew (PM), is a fungal pathogen that can severely reduce crop yields. In this study, stable overexpression and silencing demonstrated that the transcription factor VqWRKY46 was identified as a positive regulator of PM resistance in grapevine. This enhanced resistance was associated with increased callose deposition and hypersensitive response cell death. We also demonstrated that VqLIMYB specifically activated VqWRKY46 expression by binding to a CAGTTA motif in its promoter. Moreover, VqWRKY46 interacted with VqNF-YC9 and formed homodimers, positively regulating VqNF-YC9 expression by directly binding to three W-box motifs in its promoter. Additionally, DAP-Seq analysis identified VqDSC1, a TIR-NB-LRR resistance protein, as a downstream target of VqWRKY46 in grapevine. Further analysis indicated that VqWRKY46 directly bound to Wbox1 in the VqDSC1 promoter to upregulate its expression, whereas VqNF-YC9 did not bind. Interestingly, the interaction between VqNF-YC9 and VqWRKY46 enhanced the DNA binding activity of VqWRKY46 to promote the expression of VqDSC1. Furthermore, overexpressing VqLIMYB, VqNF-YC9, and VqDSC1 in Arabidopsis and/or instantaneous transformation in grapes showed that they are positive regulators of PM resistance. Collectively, our findings uncover a novel regulatory module (VqLIMYB-VqWRKY46/VqNF-YC9-VqDSC1) that mediates grapevine resistance to E. necator. This study provides new insights into the molecular basis of grapevine resistance to PM and highlights potential targets for breeding disease-resistant grapevines.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Australian chickpea pan-genome provides insights into genome organization and offers opportunities for enhancing drought adaptation for crop improvement. 澳大利亚鹰嘴豆泛基因组提供了对基因组组织的见解，并为提高作物的干旱适应能力提供了机会。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70192

Vanika Garg,Rutwik Barmukh,Yan Huang,Annapurna Chitikineni,Kristy Hobson,Bicheng Yang,Yong Jia,Shengnan Bi,Sukhjiwan Kaur,Muhammad Ahsan Asif,Matthew Hayden,Sally Norton,Darshan L Sharma,Kadambot H M Siddique,Xin Liu,Chengdao Li,Rajeev K Varshney

{"title":"An Australian chickpea pan-genome provides insights into genome organization and offers opportunities for enhancing drought adaptation for crop improvement.","authors":"Vanika Garg,Rutwik Barmukh,Yan Huang,Annapurna Chitikineni,Kristy Hobson,Bicheng Yang,Yong Jia,Shengnan Bi,Sukhjiwan Kaur,Muhammad Ahsan Asif,Matthew Hayden,Sally Norton,Darshan L Sharma,Kadambot H M Siddique,Xin Liu,Chengdao Li,Rajeev K Varshney","doi":"10.1111/pbi.70192","DOIUrl":"https://doi.org/10.1111/pbi.70192","url":null,"abstract":"Chickpea (Cicer arietinum L.) is an important legume crop that has been subjected to intensive breeding, resulting in limited genetic diversity. Australia is the world's second largest producer and the leading exporter of chickpea; the genomic architecture of its cultivars remains largely unexplored. This knowledge gap hinders efforts to enhance their genetic potential for production, protection, and stress adaptation. To address this, we generated high-quality genome assemblies and annotations for 15 leading Australian chickpea cultivars using single-tube long-fragment read technology. The pan-genome analysis identified 34 345 gene families, including 13 986 dispensable families enriched for genes associated with key agronomic traits. Comparative genomic analysis revealed ~2.5 million single-nucleotide polymorphisms, nearly 200 000 insertions/deletions, and over 280 000 structural variations. These variations were found in key flowering time genes, seed weight-related genes, and disease resistance genes, providing insights into the genetic diversity underlying these critical traits. Haplotype analysis of key genes within the 'QTL-hotspot' region revealed the absence of superior haplotypes in Australian cultivars. Validation using Kompetitive allele-specific PCR markers confirmed these findings, highlighting the need to introduce beneficial haplotypes from diverse accessions to enhance drought tolerance in Australian chickpea cultivars. The genomic resources generated in this study provide valuable insights into chickpea genetic diversity and offer potential avenues for crop improvement.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"70 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus. 一个伴侣蛋白bac01。CCT8通过影响甘蓝型油菜的生长素和茉莉酸信号传导，对硅油长度和种子重量有影响。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70184

Zhaoyang Qu,Ze Tian,Liqing Wei,Chao Wang,Lieqiong Kuang,Jiaqi Yan,Furong Wang,Nian Wang,Jinxing Tu,Xinfa Wang,Hanzhong Wang,Xiaoling Dun

{"title":"A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus.","authors":"Zhaoyang Qu,Ze Tian,Liqing Wei,Chao Wang,Lieqiong Kuang,Jiaqi Yan,Furong Wang,Nian Wang,Jinxing Tu,Xinfa Wang,Hanzhong Wang,Xiaoling Dun","doi":"10.1111/pbi.70184","DOIUrl":"https://doi.org/10.1111/pbi.70184","url":null,"abstract":"Seed weight (SW), which is directly influenced by silique length (SL), is a critical agronomic trait significantly affecting both the quality and yield of rapeseed. In this study, a shorter silique length (ssl) mutant was generated through ethyl methane sulfonate mutagenesis, exhibiting reduced SL and SW compared to the wild type. Utilizing a map-based cloning approach, BnaC01.CCT8, a member of the chaperonin containing T-complex polypeptide-1 (CCT) family, was identified as the gene responsible for restoring the ssl phenotype. A missense mutation from alanine to valine (A507V) in BnaC01.CCT8 was identified as crucial for its functional activity, as evidenced by the genetic complementation of BnaC01.CCT8 and BnaC01.CCT8A507V in the Arabidopsis cct8-2 background. Moreover, overexpression of BnaC01.CCT8 in Brassica napus significantly enhanced SL, SW and seed yield per plant. Conversely, CRISPR/Cas9-mediated bnac01.cct8 knockout lines exhibited reduced SL and SW. Transcriptome analysis and hormone content detection indicated that BnaC01.CCT8 positively regulated SL and SW primarily by modulating auxin and jasmonic acid signalling, thereby affecting the length of epidermal cells in the silique wall. Furthermore, BnaC01.CCT8 interacted with BnaA09.ARF18 (AUXIN-RESPONSE FACTOR 18), contributing to the regulation of SL and SW, while the A507V mutation disrupted this interaction. Haplotype analysis demonstrated that several SNP differences in BnaC01.CCT8 were significantly associated with variations in SL and SW among germplasm resources, revealing superior alleles of BnaC01.CCT8. The identification and functional analysis of BnaC01.CCT8 provide new insights into the mechanisms regulating SL and SW and present a valuable target for the genetic enhancement of rapeseed yield.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"44 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol oxidase silencing avoids protein cross-linking and enzymatic browning in Nicotiana benthamiana leaf extracts. 多酚氧化酶沉默避免蛋白质交联和酶褐变在烟叶提取物。

IF 10.1 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70202

Chidambareswaren Mahadevan, Emma C Watts, Kaijie Zheng, Shi-Jian Song, Renier A L van der Hoorn

引用次数: 0

Morphology effect of a novel biocompatible nucleic acid delivery nanosystem of g-C3N4@dsRNA for application in plant gene expression and plant virus disease protection. 新型生物相容性核酸传递纳米系统g-C3N4@dsRNA在植物基因表达和植物病毒病害保护中的形态学效应

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70189

Xuefeng Wei,Guangjin Fan,Song Yang,Xianchao Sun,Lin Cai

{"title":"Morphology effect of a novel biocompatible nucleic acid delivery nanosystem of g-C3N4@dsRNA for application in plant gene expression and plant virus disease protection.","authors":"Xuefeng Wei,Guangjin Fan,Song Yang,Xianchao Sun,Lin Cai","doi":"10.1111/pbi.70189","DOIUrl":"https://doi.org/10.1111/pbi.70189","url":null,"abstract":"Delivery of exogenous nucleic acid to intact plants is a desirable but challenging technology due to the dominant transport barrier posed by the plant cell wall. Here, we found that three different morphologies of g-C3N4 nanomaterials synthesized from urea can assist in the delivery of exogenous nucleic acids into mature leaf cells of Nicotiana benthamiana. Among these, g-C3N4 carbon dots (CDs) showed a higher ability to deliver exogenous nucleic acid compared to nanoporous and g-C3N4 nanosheets. The delivery ability of exogenous dsRNA and plasmid DNA by g-C3N4 could also be restrained by stomatal closure and the endocytosis pathways in plant cells. Furthermore, the coupling of g-C3N4 CDs with dsCP (CDs@dsCP), which is the dsRNA matching a specific fragment of the Coat protein-encoding gene of TMV, resulted in a superior antiviral effect compared with other morphologies of g-C3N4@dsRNAs and other loaded dsRNAs, which match Replicase, RNA-dependent Replicase or Movement protein of TMV. Significantly, a single spray of CDs@dsCP provided virus protection for at least 5 days. In addition, the g-C3N4 CDs and g-C3N4 CDs@dsRNA had no adverse effects on plant growth and development. Overall, our study presents a novel biocompatible and convenient tool for gene expression or gene silencing in intact plant leaves by spraying g-C3N4 nanomaterials encapsulated with DNA or dsRNA, the efficiency of which is affected by the morphology of the g-C3N4 nanomaterial, stomatal state and plant endocytosis pathway, and a highly promising solution for plant virus disease, which is an unsolved problem in plant disease control.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"15 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring potential strategies for haploid induction based on double fertilization in plants. 探索植物双受精诱导单倍体的潜在策略。

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-18 DOI: 10.1111/pbi.70197

Tengyu Li,Chenlei Wang,Jingwen Pan,Javaria Tabusam,Yan Li,Jinbo Yao,Wei Chen,Yazhong Wang,Wei Gao,Junkang Rong,Zeeshan Ahmad,Andreas Houben,Shouhong Zhu,Shuangxia Jin,Yongshan Zhang

引用次数: 0

Editing cis-elements of OsPHO1;2 improved phosphate transport and yield in rice 编辑OsPHO1；2顺式元件可改善水稻的磷酸盐运输和产量

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-17 DOI: 10.1111/pbi.70165

Kanika Maurya, Balaji Mani, Bhagat Singh, Ujjwal Sirohi, Aime Jaskolowski, Sandeep Sharma, Harsha Vardhan Tatiparthi, Satendra Kumar Mangrauthia, Renu Pandey, Yves Poirier, Jitender Giri

{"title":"Editing cis-elements of OsPHO1;2 improved phosphate transport and yield in rice","authors":"Kanika Maurya, Balaji Mani, Bhagat Singh, Ujjwal Sirohi, Aime Jaskolowski, Sandeep Sharma, Harsha Vardhan Tatiparthi, Satendra Kumar Mangrauthia, Renu Pandey, Yves Poirier, Jitender Giri","doi":"10.1111/pbi.70165","DOIUrl":"https://doi.org/10.1111/pbi.70165","url":null,"abstract":"Increasing grain yield is the primary goal of crop improvement, which is globally affected by the low availability of soil phosphate (Pi). Overexpressing Pi transporters to enhance Pi uptake often results in Pi toxicity and growth retardation. Despite advances in genetic engineering, targeting the cis-regulatory motifs of Pi transporters remains underexplored for understanding plant mechanisms and improving Pi status. Here, we demonstrate that the excision of the transcription inhibitor motif from the promoter of the Pi transporter OsPHO1;2 enhances its expression and increases root-to-shoot Pi transport, leading to improved grain yield. Through in silico and DNA-protein interaction studies, we show the role of the OsWRKY6 transcription factor in negatively regulating OsPHO1;2 expression by binding to the cis-regulatory element (W-box) present in its promoter. The oswrky6 knockout lines exhibit higher OsPHO1;2 expression and improved shoot Pi levels. Furthermore, we engineered the OsPHO1;2 promoter to precisely remove the W-box and enhance OsPHO1;2 expression. Phenotypic and physiological evaluations at the vegetative stage indicate that OsPHO1;2 promoter-edited (OsPHO1;2:PE) lines have increased shoot length, plant biomass and greater root-to-shoot Pi export under both low and normal P conditions. Notably, the 33P uptake assay reveals that OsPHO1;2:PE lines display enhanced root Pi uptake, supported by higher expression of root-associated Pi transporters (OsPHTs). An extensive agronomic assessment shows that OsPHO1;2:PE lines achieve increased seed and panicle numbers, thereby raising yield without affecting seed quality. Our findings provide valuable insights into the potential of promoter editing to improve Pi use and enhance crop yield.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"36 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsDUF2488 acts synergistically with OsPrx1.1, regulates ROS metabolism and promotes dehydration tolerance in rice OsDUF2488与OsPrx1.1协同作用，调控ROS代谢，促进水稻脱水耐受

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-17 DOI: 10.1111/pbi.70182

Dipak Gayen, Sunil Kumar, Pragya Barua, Nilesh Vikram Lande, Subhasis Karmakar, Amit K. Dey, Saurabh Gayali, Tushar Kanti Maiti, Kutubuddin Ali Molla, Snehal Murumkar, Subhra Chakraborty, Niranjan Chakraborty

{"title":"OsDUF2488 acts synergistically with OsPrx1.1, regulates ROS metabolism and promotes dehydration tolerance in rice","authors":"Dipak Gayen, Sunil Kumar, Pragya Barua, Nilesh Vikram Lande, Subhasis Karmakar, Amit K. Dey, Saurabh Gayali, Tushar Kanti Maiti, Kutubuddin Ali Molla, Snehal Murumkar, Subhra Chakraborty, Niranjan Chakraborty","doi":"10.1111/pbi.70182","DOIUrl":"https://doi.org/10.1111/pbi.70182","url":null,"abstract":"Stress-mediated regulation of energy metabolism and its relation to plant adaptation remain largely unknown. Mitochondrial redox potential is greatly influenced by stress-induced reactive oxygen species (ROS); therefore, we mapped the dehydration-induced alterations in the mitochondrial proteome of a resilient rice cultivar, Rasi, generating a proteome map representing the largest inventory of dehydration-responsive mitochondrial proteins from any plant species. Quantitative proteomic analysis led to the identification of an array of dehydration-responsive proteins (DRPs), associated with various cellular functions, conceivably impinging on the molecular mechanism of adaptation. One DRP identified in the mitochondrial proteome was yeast cadmium factor 54 (YCF54-like), also known as DUF (domain of unknown function) and hereafter referred to as OsDUF2488. We demonstrated that OsDUF2488 localises to mitochondria and preferentially interacts with peroxiredoxin, OsPrx1.1. Overexpression of OsDUF2488 in rice caused enhanced tolerance to dehydration and oxidative stress, while CRISPR/Cas9 knockout mutants of OsDUF2488 showed hypersensitivity to dehydration. Upon exposure to dehydration, OsDUF2488 could rescue mitochondrial dysfunction, contributing to increased ATP production in OsDUF2488-overexpressing rice. Coexpression of OsDUF2488 and OsPrx1.1 in yeast demonstrated a mutual effect on enhanced ROS catabolism, suggesting a cross-kingdom adaptive response of OsDUF2488. Our findings suggest that OsDUF2488 acts synergistically with OsPrx1.1 to regulate redox homeostasis and promote stress tolerance in rice.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"11 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A natural variation in the promoter of TaGDSL‐7D contributes to grain weight and yield in wheat TaGDSL‐7D启动子的自然变异影响小麦的粒重和产量

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1111/pbi.70204

Fei He, Dejie Du, Zhaoyan Chen, Liang Ma, Yunjie Liu, Zhaoju Li, Long Song, Zihao Jiang, Yujie Fan, Qixin Sun, Zhongfu Ni

{"title":"A natural variation in the promoter of TaGDSL‐7D contributes to grain weight and yield in wheat","authors":"Fei He, Dejie Du, Zhaoyan Chen, Liang Ma, Yunjie Liu, Zhaoju Li, Long Song, Zihao Jiang, Yujie Fan, Qixin Sun, Zhongfu Ni","doi":"10.1111/pbi.70204","DOIUrl":"https://doi.org/10.1111/pbi.70204","url":null,"abstract":"SummaryGrain weight (GW) is a critical agronomic trait that greatly impacts wheat yield, yet the underlying genetic factors remain largely unknown. Here, we identify and characterize a candidate gene TaGDSL‐7D controlling GW in wheat through map‐based cloning, which encodes an ER‐located GDSL‐family protein. Natural variations within the promoter of the GW‐enhancing TaGDSL‐7DHS2 allele result in a differential transcriptional response by promoting the binding of the TaGT1 transcription factor, a positive GW regulator. Additionally, TaGT1 directly interacts with TaBZR1 to enhance TaGDSL‐7D activation. Haplotype analysis revealed that TaGDSL‐7DHS2 is a superior haplotype, improving both GW and yield. Taken together, these findings highlight the pivotal role of TaGDSL‐7D in deciphering the genetic basis of GW in wheat and provide valuable natural allelic variation for GW improvement in high‐yield breeding programmes.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"32 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus 82.139农杆菌T-DNA基因共转化促进了杨树转基因芽的再生

IF 13.8 1区生物学

Plant Biotechnology Journal Pub Date : 2025-06-16 DOI: 10.1111/pbi.70159

Greg S. Goralogia, Cathleen Ma, David S. Taylor, Abigail Lawrence, Victoria Conrad, Ekaterina Peremyslova, Steven H. Strauss

{"title":"Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus","authors":"Greg S. Goralogia, Cathleen Ma, David S. Taylor, Abigail Lawrence, Victoria Conrad, Ekaterina Peremyslova, Steven H. Strauss","doi":"10.1111/pbi.70159","DOIUrl":"https://doi.org/10.1111/pbi.70159","url":null,"abstract":"Barriers to transformation and regeneration continue to hamper the application of recombinant DNA-based biotechnologies in most crops, including for gene editing. To tackle this problem, there has been increasing interest in morphogenic regulator genes, which aid in regeneration and are often plant developmental master regulator genes. Using a set of six genes from the T-DNA of a ‘shooty’ Agrobacterium tumefaciens strain first discovered by researchers at INRA (France) in the 1990s, we developed a co-transformation (‘altruistic’) system where these genes promote the recovery and rate of regeneration of transgenic poplars without the use of exogenous plant growth regulators. This method was more efficient (2.3x) at regenerating transgenic shoots in poplar and reduced the culturing time by approximately 6 weeks relative to the conventional approach. Resulting transgenic shoots were positive for GFP and antibiotic resistance genes but did not integrate the altruistic morphogenic genes from the second strain and were phenotypically normal. Deletion testing revealed that the hormone biosynthesis genes alone were insufficient to induce altruistic shoot production in poplar. Further mutational analysis of each gene identified 6b, in combination with iaaH, iaaM and ipt, as the major factor required for non-cell autonomous shoot proliferation. Altogether, our approach highlights the utility of leveraging Agrobacterium genes for transformation, especially through co-transformation, to avoid retaining morphogenic genes in the genomes of clonally propagated plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"23 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0