Plant Biotechnology Journal最新文献

{"title":"Introducing Pattern Recognition Receptors in Potato Confers Enhanced Resistance to Ralstonia solanacearum but Not a Vector Borne Pathogen","authors":"Tianrun Li, Filipe Sathler Meira, Esteban Jarquin Bolaños, Bella Guel, Carmen S. Padilla, Kranthi K. Mandadi, James G. Thomson, Stefanie Ranf, Gitta Coaker","doi":"10.1111/pbi.70268","DOIUrl":"https://doi.org/10.1111/pbi.70268","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"52 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria.","authors":"Christina M Gregg, Barnabas Gall, Xueqin Wang, Shoko Okada, Trevor D Rapson, Robert S Allen, Ingrid Venables, Anu Mathew, Craig C Wood","doi":"10.1111/pbi.70263","DOIUrl":"10.1111/pbi.70263","url":null,"abstract":"<p><p>Engineering nitrogenase directly into crops is a long-held aspiration in plant biotechnology. Of the three types, the Fe-only nitrogenase is a promising engineering target, as it has a simpler maturation pathway than the MoFe- and VFe-nitrogenases and does not require any heterometals for its cofactor. However, previously, we have reported that the obligate electron donor of the Fe-only nitrogenase from A. vinelandii, AnfH, is mostly insoluble when expressed in plant mitochondria. Here, we employed computational methods, the Protein Repair One-Stop Shop (PROSS) protein optimisation algorithm and Rosetta energy calculations, to design eight variants of AnfH with improved soluble expression. The amino acid substitutions chosen were predicted to lower the free energy of the native state of the protein. All eight AnfH variants, containing between 1 and 11 amino acid substitutions, were more soluble than wild-type AnfH in plant mitochondria. Of these, three variants were isolated from N. benthamiana leaf, of which AnfH variant 6 (AnfH V6, T200A T228V E241H) had the best features, being approx. 90-fold more abundant in the soluble fraction. Importantly, AnfH V6 was also functional in ¹⁵N₂ reduction with A. vinelandii AnfDKG after [Fe₄S₄] cluster reconstitution. These results show that the computational design strategy used here is a powerful approach for engineering nitrogenase into plants and more broadly to plant synthetic biology and recombinant protein production.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematically Revealing Quantitative Multi-Target Integrative Effects of Plants With Artificial Intelligence Method.","authors":"Jiang Qi-Yu, Ren Tian-Ai, Fan Xin-Yu, Zeng Hui-Yan","doi":"10.1111/pbi.70321","DOIUrl":"10.1111/pbi.70321","url":null,"abstract":"<p><p>Many plants have multiple chemical components and multiple targets, and their potential effects on diseases are the integrative effects of multiple targets. How to systematically reveal the integrated multi-targets effect of plants on diseases is not only a challenge, but also an innovation. This study developed a novel research method based on artificial intelligence and took hawthorn as an example; a deep auto-encoding neural network model was used to encode the expression levels of multiple common targets between hawthorn and atherosclerosis in each cell of the single-cell transcriptome of atherosclerotic perivascular adipose tissue (PVAT) as an integrated value (MTIS). The landscape and quantitative mapping of multi-targets potential integrated effect of plants on disease at the single-cell level would be achieved based on this innovative approach, and in-depth analysis such as MTIS comparisons, MTIS-pseudotime difference analysis, cell communication analysis, and immune infiltration analysis, was performed to reveal the potential mechanism and landscapes of hawthorn on the PVAT microenvironment of atherosclerotic. Due to many plants for disease having multiple chemical compositions and multiple targets, the novel method proposed in this study may have a wide range of applications.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA156-SPL13B Module Induces Parthenocarpy Through the Gibberellin Pathway.","authors":"Da-Ru Wang, Chun-Ling Zhang, Xun Wang, Dan-Dan Liu, Xin Liu, Guo-Lin Chen, Qing Wang, Man-Shu Qu, Kuo Yang, Chun-Xiang You, Xiao-Fei Wang","doi":"10.1111/pbi.70324","DOIUrl":"10.1111/pbi.70324","url":null,"abstract":"<p><p>Fruit development typically follows pollination and fertilisation. However, parthenocarpy, the formation of seedless fruits, represents an alternative pathway. The fruit that grows from the ovary under unpollinated and/or unfertilised conditions is termed parthenocarpic. This trait is not only desirable to consumers but also contributes to yield stability under adverse environmental conditions. In this study, we demonstrate that overexpression of miR156h regulates parthenocarpy under unpollinated conditions via a gibberellic acid (GA)-dependent pathway. MdSPL13B was found to be involved in MdmiR156h-modulated GA accumulation and parthenocarpy through directly binding to the promoters of MdKO, MdKAO2 and MdGA20ox. Furthermore, MdSPL13B was able to indirectly promote GA deactivation by binding to the promoter of MdMSI1, which mediates the histone modification of MdGA2ox8 and MdSPY. This study provides novel insight into the miR156-SPL-GA regulatory module in parthenocarpy.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanism by Which the BrFIT2-BrbHLH-BrIRT1 Module Synergistically Regulates Iron Absorption in Brassica rapa ssp. Pekinensis.","authors":"Changwei Shen, Yunduan Qin, Shuai Li, Ruixiang Chen, Xiaoli Zhang, Yuanyuan Zhang, Chunyang Feng, Yu Xu, Ranghua Yuan, Xinlei Guo, Jingping Yuan","doi":"10.1111/pbi.70334","DOIUrl":"10.1111/pbi.70334","url":null,"abstract":"<p><p>Iron is essential for plant photosynthesis and human health, yet Brassica rapa ssp. pekinensis exhibits significantly lower iron content compared to other leafy vegetables, and its iron uptake mechanisms are not well understood. This study elucidates the molecular mechanisms governing iron uptake by investigating the roles of the iron-regulated gene BrFIT2 (Bra011972) and the iron transport protein gene BrIRT1 (Bra013419) through yeast two-hybrid, bimolecular fluorescence complementation, genetic transformation and physiological-biochemical analyses, etc. Key findings include: BrFIT2 encodes a nuclear transcription factor containing an HLH domain, with its expression in roots upregulated 6.9-fold under iron-deficient conditions. Overexpression of BrFIT2 increased iron content in leaves and roots by 1.42- and 6.17-fold, respectively, whereas silencing BrFIT2 reduced leaf iron uptake by 60%-80%. Furthermore, BrFIT2 interacts with BrbHLH38/100 to form nuclear complexes that activate the expression of the iron transporter gene BrIRT1. Overexpression of BrIRT1 resulted in root iron levels 16.29-fold higher than those in wild-type plants. This study is the first to delineate the BrFIT2-BrbHLH-BrIRT1 regulatory cascade in Brassica rapa, challenging the conventional model of direct FIT-target gene interactions.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyacylated Delphinidin Biosynthesis Catalysed by PhF3'5'H-PhBGLU12-PhSCPL2 Determines the Blue Pigmentation in Cineraria (Pericallis hybrida).","authors":"Yumeng Cui, Jiangshan Ren, Jiawei Fan, Hao Li, Yi Li, Yuqing Ye, Jingwen Wu, Fangting Qi, Silan Dai, He Huang","doi":"10.1111/pbi.70330","DOIUrl":"10.1111/pbi.70330","url":null,"abstract":"<p><p>The polyacylation modification of anthocyanins constitutes a pivotal step for plants to develop persistent bright blue floral pigmentation, but the biosynthesis and regulatory mechanisms of polyacylated anthocyanin remain poorly understood. In the blue cineraria (Pericallis hybrida), an important ornamental species, a unique anthocyanin structure is characterised by 7- and 3'-polyacylated delphinidin derivatives that generate its distinctive colour. Here, we identified two modification genes PhBGLU12 and PhSCPL2 in the anthocyanin metabolic pathway, which determine the formation of polyacylated delphinidin derivatives in cineraria. RNAi-mediated silencing of these genes resulted in the transgenic lines exhibiting significantly reduced pigmentation, resulting in faded or pale purple colours in ray florets and leaves. Further investigation revealed that co-expression of PhF3'5'H and PhBGLU12 in the leaves of pink cineraria enabled the detection of multiple polyacylated modified delphinidin derivatives accumulating. The MYB activator PhMYB6 was shown to directly activate the transcription of PhF3'5'H and PhBGLU12, thereby promoting polyacylated anthocyanin biosynthesis. Electrophoretic mobility shift assay (EMSA) and dual-luciferase assay demonstrated that PhWRKY44 influences the anthocyanin metabolic pathway by directly activating the expression of PhMYB6. Notably, PhWRKY44 expression was reciprocally regulated by PhMYB6, establishing a mutually reinforcing positive feedback loop. Functional validation demonstrated that silencing PhWRKY44 reduced anthocyanin accumulation in cineraria. These findings reveal an intricate process and regulatory module for the biosynthesis of polyacylated anthocyanins and provide genetic resources for the molecular breeding of blue ornamental varieties.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MdGLK1-MdBZR1 Module Integrates Ethylene and Strigolactone Signals to Regulate Cold Tolerance via a CBF-Dependent Pathway in Apple.","authors":"Xiao-Wei Zhang, Xiu-Hong An, Rui-Rui Xu, Yi Tian, Baoyou Liu, Yuepeng Han, Jian-Ping An","doi":"10.1111/pbi.70325","DOIUrl":"10.1111/pbi.70325","url":null,"abstract":"<p><p>Although ethylene and strigolactone (SL) are key regulators of cold tolerance in plants, the molecular crosstalk between their signalling pathways is poorly understood. Here, we identified the transcription factor GOLDEN2-LIKE1 (MdGLK1) as a central integrator of ethylene and SL signalling during the apple (Malus × domestica) cold stress response. MdGLK1 enhanced cold tolerance by recruiting BRASSINAZOLE-RESISTANT1 (MdBZR1), a core component of brassinosteroid signalling, thereby promoting MdBZR1-mediated transcriptional activation of the cold-responsive genes C-REPEAT BINDING FACTOR1 (MdCBF1) and MdCBF2. Ethylene signalling modulated this process through ETHYLENE INSENSITIVE3-BINDING F-BOX PROTEIN1 (MdEBF1), which targets MdGLK1 for proteasomal degradation, thereby attenuating MdGLK1-enhanced cold tolerance. In addition, SUPPRESSOR OF MORE AXILLARY GROWTH2-LIKE8 (MdSMXL8), a repressor of SL signalling, interfered with the formation of the MdGLK1-MdBZR1 complex, further suppressing MdGLK1-mediated cold tolerance. Collectively, the MdGLK1-MdBZR1-MdCBF1/2 regulatory module operates as a signalling hub that integrates the ethylene and SL pathways during cold stress adaptation in apple. These findings elucidate the molecular mechanism by which MdGLK1 coordinates ethylene and SL signals to enhance cold tolerance, thereby offering potential targets for optimising the trade-off between growth and stress tolerance in plants.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CYP82G1 Enzyme Functions as Chromone Hydroxylase in the Biosynthesis of 6-Hydroxy-2-(2-Phenylethyl)chromone in Aquilaria sinensis.","authors":"Wenli Wu, Tingting Yan, Yuanyuan Zhao, Xuan Zhang, Iain Wilson, Gaiyun Li, Hao Wang, Wenli Mei, Ruibo Wu, Xiaochen Sun, Luyuan Jiang, Xiaoyang Jiang, Fenjuan Shao, Deyou Qiu","doi":"10.1111/pbi.70327","DOIUrl":"10.1111/pbi.70327","url":null,"abstract":"<p><p>Aquilaria sinensis, a plant of the genus Aquilaria in the family Thymelaeaceae, can form resinous and fragrant agarwood in response to wounding or infection. The resin physically occludes xylem vessels and ray parenchyma, limiting pathogen mobility and resource loss. The quality of agarwood is often evaluated using highly oxygenated 2-(2-phenylethyl)chromones, such as agarotetrol, 2-[2-(4-methoxy)phenylethyl]chromone, 4'-methoxyagarotetrol and 6,7-dimethoxy-2-(2-phenylethyl)chromone. Among the derivatives of 2-(2-phenylethyl)chromone (PEC), the majority possess a hydroxyl or methoxy group at the C-6 position of the chromone, with subsequent substitutions occurring at the C-5, C-7 and/or C-8 positions. The hydroxylation at the C-6 position is a critical step in the biosynthesis of these 2-(2-phenylethyl)chromones (PECs). In this study, we investigated the gene expression differences and accumulation patterns of PECs in two-year-old wild A. sinensis and Qi-Nan A. sinensis, using transcriptomic and LC-MS/MS analysis. Based on these analyses, we identified 52 candidate genes encoding cytochrome P450 (CYPs). Further investigations using a yeast expression system and functional analysis in Nicotiana benthamiana and A. sinensis revealed that AsCYP82G1 could hydroxylate 2-(2-phenylethyl)chromone to form 6-hydroxy-2-(2-phenylethyl)chromone. Homology modelling and molecular docking demonstrated that the residues methionine (MET) and arginine (ARG) at positions 222 and 223 of the AsCYP82G1 protein are likely to play an important role in the catalysis of 2-(2-phenylethyl)chromone to 6-hydroxy-2-(2-phenylethyl)chromone. Finally, the reaction mechanism of AsCYP82G1 was studied by quantum mechanical calculation. Our research provides new insights into the molecular mechanism of PECs biosynthesis and lays the foundation for the breeding of high-quality agarwood varieties and large-scale production of PECs using synthetic biology.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucuronoyl Esterase Expressed in Aspen Xylem Affects γ-Ester Linkages Between Lignin and Glucuronoxylan Reducing Recalcitrance and Accelerating Growth.","authors":"Marta Derba-Maceluch,Laura García Romañach,Mattias Hedenström,Madhusree Mitra,Evgeniy N Donev,János Urbancsok,Zakiya Yassin,Madhavi L Gandla,Pramod Sivan,Jan Šimura,Gerhard Scheepers,Leif J Jönsson,Francisco Vilaplana,Ewa J Mellerowicz","doi":"10.1111/pbi.70301","DOIUrl":"https://doi.org/10.1111/pbi.70301","url":null,"abstract":"Wood is the most abundant renewable natural resource composed of different polysaccharides and lignin, but its utilisation is hampered by intermolecular linkages between these components forming lignin-carbohydrate complexes (LCCs) causing recalcitrance. The links between glucuronoxylan and the γ-C of lignin (γ-ester linkages) are thought to contribute to one-third of LCCs, but direct evidence for their natural occurrence and their role in recalcitrance has been scarce so far. To address these issues, Phanerochaete carnosa glucuronoyl esterase (PcGCE), hydrolysing γ-ester linkages, was expressed in cell walls of developing wood in hybrid aspen (Populus tremula L. × tremuloides Michx.). The enzyme reduced HSQC 2D NMR signals corresponding to the γ-esters and xylan in dioxane-extracted LCCs without altering glucuronoxylan content or structure. This increased acid solubility of lignin and lignin content. Reduced wood recalcitrance was shown by increased sugar yields and glucose production rates (by approx. 20%) in saccharification without pretreatment and increased xylan extractability by subcritical water (by approx. 70%). Moreover, trees expressing PcGCE exhibited greater primary and secondary growth. Transcriptomics and metabolomics analyses in developing wood suggested that growth could have been induced by a higher transcription of SMR2 and RPOTmp, which was likely triggered by the secondary cell wall integrity signalling. The results provide evidence for the natural existence of LCC γ-esters and their significant contribution to lignocellulose recalcitrance. Furthermore, they show that reducing γ-ester linkages could increase plant productivity.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"17 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competition Binding of CmbHLH1L and CmNLP6/7L to CmbHLH63 Modulates Leaf Senescence in Chrysanthemum.","authors":"Yuna Yang, Ziyan Qian, Yinglin Li, Rui Liu, Hong Qiao, Zhengyao Shao, Debao Huang, Jiafu Jiang, Fadi Chen, Sumei Chen, Likai Wang","doi":"10.1111/pbi.70308","DOIUrl":"https://doi.org/10.1111/pbi.70308","url":null,"abstract":"<p><p>Leaf senescence impacts the quality, productivity and post-harvest performance of crops and ornamental plants. However, the underlying molecular mechanisms governing this process remain poorly understood. In this study, we identified CmbHLH63 as a 'brake signal' of leaf senescence in chrysanthemum and elucidated its critical regulatory role within the CmbHLH1L-CmbHLH63-CmNLP6/7L complex. CmbHLH63 promotes leaf senescence, while it is downregulated during this process. Notably, CmbHLH63 lacks intrinsic transcriptional regulatory activity; however, the expression of a majority of genes was repressed in plants overexpressing CmbHLH63. Further investigation revealed that this repression is achieved through recruiting the transcriptional repressor CmbHLH1L. We further found that CmNLP6/7L, a transcriptional activator, competes with CmbHLH1L for the interaction with CmbHLH63, leading to the release of their inhibition on downstream gene CmNLP6/7L. We found that the accumulation of CmbHLH1L is increased in the cytoplasm and decreased in the nucleus during leaf senescence. Mass spectrometry assays and subcellular localization studies revealed that CmbHLH1L undergoes lysine acetylation at position 140, which prevents its nuclear accumulation, thereby elevating CmNLP6/7L expression during leaf senescence.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}