Plant Biotechnology Journal最新文献

{"title":"A New Breeding Technique for F1 Hybrid Production From Self-Incompatible Species.","authors":"Rowan P Herridge,Prasanthi Namburi,Shiny Varghese,Richard C Macknight,Lynette R Brownfield","doi":"10.1111/pbi.70632","DOIUrl":"https://doi.org/10.1111/pbi.70632","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"4 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmWRKY29 Transcriptionally Represses ZmRBOHC to Attenuate ROS Production and Facilitates Gibberella Stalk Rot Susceptibility in Maize.","authors":"Jingye Fu,Li Zhu,Luyao Wang,Wenzheng Pei,Chenying Zhu,Fukun Bao,Yuhan Zhang,Gang Chen,Qiang Wang","doi":"10.1111/pbi.70629","DOIUrl":"https://doi.org/10.1111/pbi.70629","url":null,"abstract":"Gibberella stalk rot (GSR), caused by the fungal pathogen Fusarium graminearum, severely threatens maize production. However, the molecular mechanisms underlying maize resistance to GSR remain poorly understood. Here, we have identified ZmWRKY29 as a transcriptional repressor induced by F. graminearum infection, which negatively regulates maize resistance to GSR. Overexpression of ZmWRKY29 significantly compromised GSR resistance with a marked reduction in reactive oxygen species (ROS) production and callose deposition, while its mutation enhanced resistance and elevated ROS accumulation. RNA-seq revealed that the expression levels of two respiratory burst oxidase homologues, ZmRBOHA and ZmRBOHC, were decreased in ZmWRKY29-OE lines following F. graminearum challenge. ZmWRKY29 directly binds to the W-boxes in the promoters of ZmRBOHA and ZmRBOHC to repress their transcription. Moreover, mutation of ZmRBOHC similarly led to increased susceptibility and diminished ROS accumulation, confirming that ZmWRKY29-targeted ZmRBOHC is required for ROS production and GSR resistance. Thus, ZmWRKY29 acts as a susceptibility factor, repressing GSR resistance possibly by inhibiting ZmRBOHC-mediated ROS production. This highlights a pathogen strategy of exploiting host transcriptional machinery to suppress ROS defences and facilitate infection.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"6 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Maternal Haploid Induction by Disrupting KOKOPELLI in Medicago truncatula.","authors":"Na Wang,Yan Feng,Jinting Cao,Yiran Gao,Yanxi Pei,Jiangqi Wen,Kirankumar S Mysore,Hao Lin,Lifang Niu","doi":"10.1111/pbi.70628","DOIUrl":"https://doi.org/10.1111/pbi.70628","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"20 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147359304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System.","authors":"Jing-Jing Li,Yi-Mei Zhao,Jia-Fan Liu,Chen-He Li,Hui Zhang,Yi-Tong Song,Quan Zuo,Pei-Yi Wang,Kai Gao,Dong Meng,Shi-Hui Niu","doi":"10.1111/pbi.70571","DOIUrl":"https://doi.org/10.1111/pbi.70571","url":null,"abstract":"Conifers serve as the cornerstone of global forest ecosystems, yet their genetic transformation faces notorious challenges. To overcome the intrinsic resistance of conifer adventitious roots to Agrobacterium/Rhizobium-mediated transformation, we systematically and iteratively engineered the binary vector by introducing chimeric Ri plasmid derived T-DNA borders, a phosphomimetic VirG mutant (VirGN54D) and a hyperactive replication origin mutant (pVS1 R106H). This optimised system enabled efficient, cross-species genetic transformation in diverse plants, including the recalcitrant gymnosperm Ginkgo biloba, thus establishing a broadly applicable genetic toolkit for plant research. Leveraging this system, we established a non-aseptic efficient root transformation system for Pinus tabuliformis. Given that conifers are evolutionarily ancient gymnosperms, this readily accessible system provides an unprecedented window to probe fundamental questions in functional genomics and evolutionary mechanisms, such as the dynamics of long-distance macromolecular trafficking. Transgenic deployment of Arabidopsis florigen FT in Chinese pine roots uncovered conserved protein level long-distance mobility, despite lacking functional FT orthologs in conifer genomes. Conversely, the conifer age biomarker DAL1 exhibited no detectable mobility even when engineered fusion with tRNA-like sequence (TLS) tag, which has been shown to facilitate long-distance mRNA transport in angiosperms, exposing lineage-specific constraints on mobile mRNA. Our work provides a versatile genetic tool for challenging plant species and offers new insights into long-distance signalling evolution. The streamlined protocols and universal vector system address critical bottlenecks in conifer biotechnology and open avenues for functional genomics in other non-model plants.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147329603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNAwhisper: An Integrated Deep Learning Pyramidal Framework for Multi-Trait Genomic Prediction and Adaptive Marker Prioritisation.","authors":"Yuexin Ma, Xiang Li, Xiaohao Ji, Chunying Wang, Di Zhang, Tingting Zhai, Haibo Wang, Ping Liu","doi":"10.1111/pbi.70619","DOIUrl":"https://doi.org/10.1111/pbi.70619","url":null,"abstract":"<p><p>Genomic selection (GS) is critical for accelerating genetic gain in modern plant breeding. Deep learning approaches offer powerful non-linear representation capabilities for modelling non-additive effects. However, their application in GS remains restricted, as high-dimensional, low-sample and noisy data hinder the identification of informative markers. The present study proposes DNAwhisper, a deep learning framework designed for multi-trait prediction and adaptive marker prioritisation. The framework integrates a cascaded architecture, GFIformer, employing shared network parameters across partitioned marker blocks to adaptively compress genetic features within a hierarchical pyramid. Pre-training on population genetic structure regularises feature learning to establish a generalisable latent representation. During trait modelling, importance scores for aggregated genomic regions at multi-resolutions are extracted from the distinct pyramid levels under trait-guided deep supervision, enhancing interpretability and supporting marker prioritisation. DNAwhisper was evaluated on maize, wheat, tomato and grape datasets for marker prioritisation and phenotypic prediction, achieving prediction accuracy approximately 3.0% to 10.0% higher than the baseline model. Furthermore, DNAwhisper identifies major QTLs (e.g., <math> <semantics><mrow><mi>VGT</mi> <mn>1</mn></mrow> <annotation>$$ VGT1 $$</annotation></semantics> </math> , <math> <semantics><mrow><mi>ZCN</mi> <mn>8</mn></mrow> <annotation>$$ ZCN8 $$</annotation></semantics> </math> ) and epistatic signals within the gibberellin metabolic pathway across maize flowering traits. This framework provides a new strategy for dissecting the genetic architecture of complex traits.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147300418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psyllid Cysteine Cathepsins Directly Cleave the Outer Membrane Protein BamD of Citrus Huanglongbing Pathogen.","authors":"Yu Du, Yu Bin, Jinhua Yang, Manman Hu, Liqin Jiang, Qiaoling Su, Shiyi Lin, Lanfeng Qu, You Li, Taiyun Wei","doi":"10.1111/pbi.70610","DOIUrl":"https://doi.org/10.1111/pbi.70610","url":null,"abstract":"<p><p>Candidatus Liberibacter asiaticus (CLas), the causative agent of citrus Huanglongbing (HLB), is transmitted by Asian citrus psyllid, Diaphorina citri; however, the role of the insect's immune effectors in defending against CLas remains poorly understood. This study reveals that D. citri cathepsin L (DcCTSL1), a member of the cysteine protease family, serves as a key immune effector directly cleaving CLas outer membrane protein BamD, thereby defending against CLas infection in insect vectors. Upon CLas infection, DcCTSL1 expression is upregulated. This protease targets the CLas membrane by specifically interacting with BamD, which in turn enables DcCTSL1 to recognise the conserved Ile145-Arg146 motif of BamD. Following this recognition, DcCTSL1 cleaves the peptide bond between Arg146 and Asp147 of BamD, an action that is mechanistically dependent on the conserved catalytic triad (Cys185, His325, and Asn350) of DcCTSL1. Heterologous application of DcCTSL1 in citrus plants confers resistance to CLas. Furthermore, a homologous citrus cysteine protease also cleaves BamD via the same mechanism. Our study reveals an evolutionarily conserved defence mechanism mediated by cysteine proteases against CLas in both insect vectors and plant hosts, thus offering a novel foundation for developing protease-based strategies against HLB.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":" ","pages":""},"PeriodicalIF":10.5,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147315853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of carbon and nitrogen utilization for enhanced photosynthesis, biomass and yield by coexpression of phosphoenolpyruvate carboxylase, aspartate aminotransferase and glutamine synthetase in Brassica juncea","authors":"Mamta,&nbsp;Shiv Shanker Pandey,&nbsp;Anish Kaachra,&nbsp;Sachin Vashisath,&nbsp;Dinesh Kumar,&nbsp;Sanjay Kumar","doi":"10.1111/pbi.70120","DOIUrl":"10.1111/pbi.70120","url":null,"abstract":"<p>Coexpression of genes involved in carbon (C) and nitrogen (N) metabolism offers a promising avenue for improving crop yield. This study investigated the impact of coexpressing <i>phosphoenolpyruvate carboxylase</i> [<i>ZmPEPC</i> (<i>P</i>)], <i>aspartate aminotransferase</i> [<i>GmAspAT</i> (<i>A</i>)] and <i>glutamine synthetase</i> [<i>NtGS</i> (<i>G</i>)] in <i>Brassica juncea</i> to enhance plant yield through improved C and N utilization. Plants coexpressing three genes (<i>BjPAG</i>) showed a significant increase in plant growth, biomass, chlorophyll and carotenoid content, while exhibiting decreased anthocyanin and hydrogen peroxide production. These improvements were observed under both ambient (~400 ppm; ACO₂) and low (~200 ppm; LCO₂) atmospheric CO₂ conditions. Chlorophyll fluorescence study revealed that <i>BjPAG</i> plants had higher photosynthetic efficiency, particularly increased maximum quantum yield of PSII and performance index. This facilitated increased CO₂ assimilation, total soluble sugar and starch accumulation, along with altered stomatal traits under both ACO₂ and LCO₂ conditions. Additionally, <i>BjPAG</i> plants accumulated less ammonium than control plants under both CO₂ levels, indicating effective functioning of the overexpressed genes. Improved performance (enhanced photosynthetic rates, biomass and seed yield) of <i>BjPAG</i> plants under various N environments (6, 3 and 0.3 m<span>m</span> N) also supported improved N utilization. NMR analysis suggested that <i>PAG</i>-coexpression resulted in a higher flux of assimilated CO₂ and NH₃ towards sugars, amino acids, organic acids, polyphenols and glucosinolates. In conclusion, this study highlights the effectiveness of coexpressing <i>ZmPEPC</i>, <i>GmAspAT</i> and <i>NtGS</i> in <i>B. juncea</i>, leading to efficient C and N utilization, ultimately resulting in enhanced photosynthesis, plant biomass and seed yields, and as a promising strategy for increasing the yield of food crops.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 3","pages":"1110-1132"},"PeriodicalIF":10.5,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Variation in TaFAD8-D Promoter Enhances Thermotolerance in Wheat Through Fatty Acid and Lipid Remodelling","authors":"Hongjian Yu,&nbsp;Tianyu Lan,&nbsp;Weiwei Mao,&nbsp;Yongfa Wang,&nbsp;Xiaoyu Zhang,&nbsp;Mengsi Ma,&nbsp;Shuo Chen,&nbsp;Guang Chen,&nbsp;Qiang Li,&nbsp;Zhaorong Hu,&nbsp;Mingming Xin,&nbsp;Yingyin Yao,&nbsp;Weilong Guo,&nbsp;Zhongfu Ni,&nbsp;Qixin Sun,&nbsp;Huiru Peng","doi":"10.1111/pbi.70397","DOIUrl":"10.1111/pbi.70397","url":null,"abstract":"<p>Heat stress (HS) has become an increasing threat to wheat productivity under global warming. However, the genetic loci for thermotolerance and the underlying molecular mechanisms remain largely unknown. In this study, genetic mapping identified a thermotolerance locus, <i>QMpe.cau-2D</i>, encoding fatty acid desaturase 8 (FAD8), with the transposable element (TE) insertions present in the promoter region in the thermotolerant cultivar. The expression of <i>TaFAD8-D</i> was negatively associated with thermotolerance. Loss-of-function mutations in <i>TaFAD8</i> enhanced photosynthetic efficiency, seedling survival rate, and thousand-grain weight under HS. Transcriptome, fatty acid, and lipid profiling analyses showed that <i>TaFAD8</i> mutation affected the expression of genes involved in lipid biosynthesis and metabolism to mediate the fatty acid composition and lipid remodelling, thereby maintaining chloroplast membrane fluidity and integrity under HS. TaWRKY71 negatively regulated the transcription of <i>TaFAD8</i> by binding to its promoter, and mutation of <i>TaWRKY71</i> reduced photosynthetic efficiency under HS. Our findings identify a beneficial <i>TaFAD8-D</i> haplotype, uncover its molecular mechanism and regulatory pathways in heat response, and provide a strategy for breeding climate-resilient wheat varieties.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 3","pages":"1251-1268"},"PeriodicalIF":10.5,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145282786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RrMYB2 Regulates Drought Stress via RrJMJ12-Dependent Epigenetic Modification in Rosa rugosa","authors":"Mengjuan Bai,&nbsp;Yating Yang,&nbsp;Yunfeng Gao,&nbsp;Mengmeng Xu,&nbsp;Qianxiang Zhang,&nbsp;Shuo Liu,&nbsp;Jun Lu,&nbsp;Jianwen Wang,&nbsp;Changquan Wang,&nbsp;Liguo Feng","doi":"10.1111/pbi.70432","DOIUrl":"10.1111/pbi.70432","url":null,"abstract":"<p><i>Rosa rugosa</i> (<i>R. rugosa</i>), an economically important crop valued for its fragrance and medicinal properties, is highly susceptible to drought stress under open-field cultivation. However, the molecular mechanisms underlying its drought response remain largely unexplored. Here, we identified RrMYB2, a transcription factor that positively regulates drought tolerance in <i>R. rugosa</i>. Overexpressing <i>RrMYB2</i> in <i>Arabidopsis thaliana</i> and <i>R. rugosa</i> enhanced drought tolerance. Virus-induced silencing of <i>RrMYB2</i> plants exhibited impaired stomatal closure and reduced drought resistance. RNA-seq of silenced plants revealed that <i>protein phosphatases type 2C</i> (<i>PP2C</i>) genes, including <i>RrHAB1</i>, <i>RrHAB2</i> and <i>RrABI1</i>, were significantly up-regulated. Biochemical analyses demonstrated that the negative regulation of <i>PP2Cs</i> by RrMYB2 was dependent on its interaction protein RrJMJ12, an H3K27me3 histone demethylase and genetic evidence indicated that <i>RrJMJ12</i> acts downstream of <i>RrMYB2</i> to negatively regulate drought resistance. Moreover, the RrMYB2-RrJMJ12 protein complex reduced RrJMJ12 binding to the CTCTGYTY motifs in the promoters of <i>PP2C</i> genes, with this inhibitory effect further enhanced under drought conditions. Collectively, our findings reveal a novel molecular mechanism in which a transcription factor cooperates with an epigenetic modifier to regulate drought tolerance in <i>R. rugosa</i>.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 3","pages":"1578-1594"},"PeriodicalIF":10.5,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145373982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgene-Free, Gene-Edited Cavendish Bananas (Musa acuminata, AAA)","authors":"Maiko Kato,&nbsp;Jen Kleidon,&nbsp;Amba Phillips,&nbsp;Alexandra Tabrett,&nbsp;Robert Harding,&nbsp;James Dale,&nbsp;Jean-Yves Paul","doi":"10.1111/pbi.70429","DOIUrl":"10.1111/pbi.70429","url":null,"abstract":"<p>Global consumer acceptance of gene-edited food crops is increasing with new breeding technologies that can modify the genome without foreign DNA integration. Here, we report an <i>Agrobacterium</i>-based system for transgene-free, gene editing of the banana cultivar, Cavendish. The protocol uses a three-tiered approach whereby cells containing T-DNA are enriched by positive antibiotic selection over a 48-h period, CRISPR/Cas9-mediated gene editing occurs over a short transient window and negative selection of cells containing T-DNA is achieved by the addition of 5-FC, which is converted to cytotoxic 5-FU by the co-expressed CODA enzyme. Two key enzymes in the carotenoid biosynthesis pathway were targeted as visual markers of editing: <i>phytoene desaturase</i> (<i>pds</i>) and <i>lycopene β-cyclase</i> (<i>LCYb</i>). Disruptive edits of the <i>LCYb</i> gene were easily identifiable at the calli stage of the regeneration process with cells appearing pink due to lycopene accumulation. Eight of 32 plants for <i>pds</i> and 34 of 125 plants for <i>LCYb</i> contained edits and were likely free of integrated vector DNA as determined by targeted genome sequencing and T-DNA-specific PCR, respectively. Plants containing disruptive tri-allelic edits in either gene displayed an albino phenotype. A subset of potentially transgene-free, edited plants was verified by whole genome sequencing. The transient editing protocol has an estimated 17.6% to 21.9% efficiency in generating plants containing disruptive tri-allelic modifications that are free of novel DNA. The protocol overcomes the regulatory limitations associated with the release of gene-edited, vegetatively propagated crops and provides an effective means of creating new disease-resistant and agronomically superior Cavendish cultivars.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 3","pages":"1620-1634"},"PeriodicalIF":10.5,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70429","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145381019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}