Plant Biotechnology Journal最新文献

{"title":"Ectopic Expression of ScALDH21 From a Desert Moss Enhances Cotton Resistance to Verticillium Wilt via the Modulation of Jasmonates and Phenylpropanoid Pathways","authors":"Honglan Yang,&nbsp;Xiaoshuang Li,&nbsp;Tohir A. Bozorov,&nbsp;Jianwei Zhang,&nbsp;Aerguli Jiamahate,&nbsp;Dawei Zhang,&nbsp;Jiancheng Wang,&nbsp;Qilin Yang,&nbsp;Zongrang Liu,&nbsp;Dina Mahesati,&nbsp;Haijiang Xu,&nbsp;Shuangxia Jin,&nbsp;Daoyuan Zhang,&nbsp;Yongqiang Dai","doi":"10.1111/pbi.70669","DOIUrl":"10.1111/pbi.70669","url":null,"abstract":"<p>Biotic stresses, particularly Verticillium wilt (VW), lead to a global decline in cotton yields. Here, we demonstrate that ectopic expression of <i>ScALDH21</i>, a gene from the desiccation-tolerant moss <i>Syntrichia caninervis</i> Mitt. and absent in angiosperms, enhances cotton's resistance to VW. Multi-year, multiple location field evaluations showed that transgenic cotton lines consistently exhibited two major advantages: markedly improved resistance to VW, and significantly reduced yield loss, with an approximate 23.8% yield increase relative to non-transgenic counterparts under pathogen infection conditions. This disease resistance is associated with enhanced capacity of the transgenic lines to scavenge reactive oxygen species (ROS), induced by pathogen infection. This finding aligns with the <i>ScALDH21</i>-conferred detoxification function. Transcriptome analyses revealed a significant alteration in expression pattern of those genes that regulate phenylpropanoid and jasmonic acid (JA) pathways. Correspondingly, the accumulation of lignin and defence-related metabolites (e.g., rutin, cyanidin and jasmonates) significantly increased, suggesting that <i>ScALDH21</i>-mediated activation of the phenylpropanoid and JA pathways contributes to enhanced resistance. Analyses of ScALDH21 binding activity using CUT&amp;Tag and EMSA assays showed that it can bind to specific gene promoters within the cotton genome, highlighting that ScALDH21 not only catalyses the detoxification of aldehydes but also gains transcriptional regulatory roles. In summary, we demonstrate that expression of the heterologous <i>ScALDH21</i> in cotton leads to enhancement of resistance to VW and elucidated the mechanism. Our findings further demonstrate a promising strategy to improve biotic resistance in crops by utilizing unique functional genes from evolutionarily distant species in extreme environments.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 8","pages":"4916-4932"},"PeriodicalIF":12.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Tetraploa Genome and Multi-Omics Analysis Reveal Key Plant-Microbe-Soil Interactions for Salt Tolerance and Yield Improvement of Wheat","authors":"Cheng-Wei Qiu,&nbsp;Shuo Zhang,&nbsp;Zi-Feng Gao,&nbsp;Zhong-Hua Chen,&nbsp;Chulong Zhang,&nbsp;Mohamed Abdelalim Ali,&nbsp;Feibo Wu","doi":"10.1111/pbi.70663","DOIUrl":"10.1111/pbi.70663","url":null,"abstract":"<p>Salinity is a major threat to global agricultural productivity of staple crops such as wheat. Although microbial-based solutions hold promise for alleviating salinity stress, practical implementation is hindered by insufficient mechanistic characterization of bioinoculants and their interactions with plants. Here, we assembled the first complete reference genome of a halotolerant strain within the genus <i>Tetraploa</i>—the endophytic fungus <i>Tetraploa</i> sp. E00680. This novel genomic resource serves as a foundation for exploring previously uncharacterised salt tolerance mechanisms in this potential fungal inoculant. Our research demonstrates that E00680 enhances wheat yield under both controlled and field saline conditions. We found that E00680 systematically modulates the plant-microbe-soil interactions by optimizing rhizosphere microbial communities, increasing nutrient bioavailability, and triggering coordinated transcriptional and metabolic reprogramming in wheat. Notably, E00680 expands tryptophan metabolism to synergistically boost auxin biosynthesis in wheat by supplying precursors and activating relevant metabolic pathways. This cross-kingdom metabolic coupling facilitates better growth and salt tolerance in wheat plants. Our findings offer multi-omics and rhizosphere-level insights that can guide the development of microbial inoculants to enhance climate-resilient and sustainable crop production.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 8","pages":"4748-4765"},"PeriodicalIF":12.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “AaSIZ1-Mediated SUMOylation of AaMYB31 Positively Regulates Freezing Tolerance in Actinidia arguta”","authors":"","doi":"10.1111/pbi.70707","DOIUrl":"10.1111/pbi.70707","url":null,"abstract":"<p>\u0000 <span>Liu, L. Y.</span>, <span>L. Cao</span>, <span>N. Tang</span>, <span>W. M. Ren</span>, <span>Z. L. Zhu</span>, <span>R. Wang</span>, <span>M. H. Xiang</span>, <span>J. L. Sun</span>, <span>X. Zhang</span>, <span>F. Zhang</span>, <span>Y. Z. Lin</span>, <span>Y. T. Kong</span>, <span>Z. H. Jiang</span>, <span>L. H. Wang</span>, <span>Y. S. Liu</span>, <span>C. Zhang</span> and <span>P. P. Zheng</span> <span>2026</span>. “ <span>AaSIZ1-Mediated SUMOylation of AaMYB31 Positively Regulates Freezing Tolerance in <i>Actinidia arguta</i></span>.” <i>Plant Biotechnology Journal</i> <span>24</span>: <span>3897</span>–<span>3921</span>.\u0000 </p><p>In the above article, the authors would like to correct Figure 5D. In Figure 5D, the position between WT and <i>AaMYB31.2-OE6</i> before freezing treatment was incorrect, and the correct Figure 5D is shown below.</p><p>We apologize for this error.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 8","pages":""},"PeriodicalIF":12.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148313884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Regulation of Silicon Accumulation in Peduncle Confers Sheathed Spike in Barley","authors":"Wenxue Wu,&nbsp;Congcong Jiang,&nbsp;Guangqi Gao,&nbsp;Namiki Mitani-Ueno,&nbsp;Yu Guo,&nbsp;Jinhong Kan,&nbsp;Yehui Xiong,&nbsp;Martin Mascher,&nbsp;Zhongfu Ni,&nbsp;Jian Feng Ma,&nbsp;Ke Wang,&nbsp;Ping Yang","doi":"10.1111/pbi.70666","DOIUrl":"10.1111/pbi.70666","url":null,"abstract":"<p>Peduncle, the uppermost internode in cereals, connects the stem to the inflorescence and is critical for the transport of water, nutrients and photosynthetic assimilates. While peduncle length associates with plant height and its elongation is primarily regulated by phytohormones, we report a previously unrecognized mechanism involving the spatial distribution of silicon (Si). We identified a barley mutant, <i>sheathed spike 2</i> (<i>ss2</i>), characterized by a specifically shortened peduncle that traps the spike within the flag leaf sheath. Positional cloning and analysis of allelic mutants revealed that the wild-type <i>SS2</i> gene encodes a putative silicon efflux transporter. <i>SS2</i> is expressed throughout the lifecycle, with higher transcriptional levels in the rachis and stem internodes, and its encoded protein localizes to the plasma membrane. We demonstrate that SS2 is required for polarized Si partitioning. Unlike wild-type plants, which ultimately deposit Si in spikes, the <i>ss2</i> mutant exhibits an 8-fold increase in Si accumulation in the peduncle and a significant increase in the flag leaf. Hydroponic experiments without Si supply restored normal peduncle elongation in the <i>ss2</i> mutant, demonstrating that local Si hyper-accumulation directly inhibits elongation. The conserved role of <i>SS2</i> was supported by diversity analysis across barley and common wheat, as well as by the similar sheathed spike phenotype in tetraploid wheat lines carrying non-functional <i>SS2</i> homologues. Collectively, our findings uncover an evolutionarily conserved, silicon-dependent mechanism that regulates peduncle elongation and spike emergence in Triticeae crops like barley and wheat.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 8","pages":"4766-4779"},"PeriodicalIF":12.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70666","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147630295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PbrIDD2-PbrAPX14 Module Functions in the Ethylene-Mediated Ripening and Senescence Process of Pear Fruit","authors":"Libin Wang,&nbsp;Junpeng Niu,&nbsp;Xiaoyu Tan,&nbsp;Yuhua Xie,&nbsp;Li Jiang,&nbsp;Chen Huan,&nbsp;Xu Zhang,&nbsp;Weiqi Luo,&nbsp;Bing Xie,&nbsp;Xiaopu Ren,&nbsp;Guodong Wang,&nbsp;Yuanyuan Guo,&nbsp;Shaoling Zhang","doi":"10.1111/pbi.70664","DOIUrl":"10.1111/pbi.70664","url":null,"abstract":"<p>Endogenous H₂O₂ participated in the ethylene-dependent ripening and senescence process of horticultural fruit as a secondary messenger; however, the molecular mechanism beneath such a phenomenon has not been fully clarified until recently. By a conjoint analysis of metabolite, enzyme activities, gene expression profiles in AsA-GSH cycle of ‘Kolar’ pear, <i>PbrAPX14</i> might act as a negative factor in the ethylene-mediated H₂O₂ accumulation. PbrAPX14, located in cytosol, would reduce H₂O₂ in vitro and in vivo, inhibit ethylene production, and thus fruit ripening and senescence. After analysing the expression profiles of the differentially expressed transcription factors (TFs) followed by experimental validation, the nuclear PbrIDD2 could directly bind to the <i>cis</i>-acting element (core motif: TTTGTCG) in <i>PbrAPX14</i> promoter, activate its expression and thus enhance the H₂O₂-scavenging capacity of fruit/calli, which was associated with the mitigated ethylene evolution and fruit ripening and senescence. Further study explored that the H₂O₂-mediated post-translational S-sulfenylation of Cys⁴⁸ residue in PbrAPX14, which mitigated its function, existed in vitro and in vivo, and was upregulated by ethylene, facilitating endogenous H₂O₂ accumulation. Overall, our results implied that both transcriptional and post-translational regulation of PbrAPX14, which were (in)directly under the control of ethylene, functioned in pear ripening and senescence process via regulating endogenous H₂O₂ level.</p>","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"24 8","pages":"4878-4896"},"PeriodicalIF":12.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pbi.70664","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147751347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification","authors":"Alessandro Occhialini, Xinlu Chen, Samantha A. Miller, Mohammad Majdi, Ivette A. Fuentes Quispe, Gabriella King, Feng Chen","doi":"10.1111/pbi.70729","DOIUrl":"https://doi.org/10.1111/pbi.70729","url":null,"abstract":"Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with all reported studies restricted to the model plant <jats:italic>Nicotiana</jats:italic> . Here we report successful chloroplast genome engineering for diterpene production in the crop plant potato ( <jats:styled-content style=\"fixed-case\"> <jats:italic>Solanum tuberosum</jats:italic> </jats:styled-content> ) guided by evolutionary principles. First, we identified the <jats:italic>trnT/trnL</jats:italic> plastomic locus as a new transgene integration site with minimal integration‐associated growth penalties. Insertion of a bifunctional diterpene synthase gene from a fern that is absent in flowering plants into this plastomic site yielded transplastomic potato plants with successful production of new diterpenes, but with reduced growth. The co‐expression of an algal geranylgeranyl diphosphate synthase gene of chloroplast genome origin to enhance precursor supply restored normal growth while elevating diterpene production. Transplastomic plants were otherwise agronomically comparable to wild‐type. This work expands chloroplast engineering as a viable strategy for evolution‐inspired terpene pathway engineering in crop improvement and high‐value terpene production.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"51 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA ‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs","authors":"Malihe Mirzaee, Corinne Best, Evelyn V. Wachowski, Pal Maliga","doi":"10.1111/pbi.70728","DOIUrl":"https://doi.org/10.1111/pbi.70728","url":null,"abstract":"Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or a hepatitis delta virus (HDV) ribozyme. Cas9 is expressed from a nuclear gene and is targeted to chloroplasts by fusion to a transit peptide. Cas9 incorporated the sgRNA and introduced double‐strand breaks in the plastid DNA (ptDNA). We report here that the double‐strand DNA break in the <jats:italic>ndhA</jats:italic> and <jats:italic>rpoC1</jats:italic> genes was repaired by microhomology‐mediated end joining (MMEJ), resulting in deletions in the ptDNA. We further showed that nuclear‐expressed sgRNA can be delivered into chloroplasts by fusion with a viroid RNA, as one possible approach for RNA‐guided engineering of the ptDNA without direct chloroplast genome transformation. These results are the first step of RNA‐guided editing of the chloroplast genome in any crop.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"11 8 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148459339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GhSCAMP13 Negatively Modulates Salt Tolerance Through GhNHX7b-Dependent Na+ Homeostasis in Cotton.","authors":"Haohong Tang,Zhaojie He,Cong Sun,Haonan Shi,Zhan Gao,Yanlong Jin,Wenting Ji,Linfu Feng,Ning Wang,Zunzhe Tian,Guanjing Hu,Jie Sun,Xianpeng Xiong","doi":"10.1111/pbi.70725","DOIUrl":"https://doi.org/10.1111/pbi.70725","url":null,"abstract":"","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"26 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148451773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Immunogenicity of a Nanoparticle Combination Vaccine for Salmonella in Poultry","authors":"Shabnam Shamriz, Angelo Kaldis, Carly A. Charron, Jordan T. VanderBurgt, Christopher P. Garnham, Moussa S. Diarra, Rima Menassa","doi":"10.1111/pbi.70717","DOIUrl":"https://doi.org/10.1111/pbi.70717","url":null,"abstract":"<jats:styled-content style=\"fixed-case\"> <jats:italic>Salmonella enterica</jats:italic> </jats:styled-content> serovars ( <jats:italic>Salmonella</jats:italic> ) are common causes of bacterial gastroenteritis (salmonellosis) often associated with the consumption of poultry products. The control of <jats:italic>Salmonella</jats:italic> in broiler chickens is difficult because colonized birds may carry this pathogen undetected into the slaughterhouse and cause meat contamination. Poultry vaccines can mitigate colonization, and one promising production method is via plants. Iron is an essential nutrient for <jats:italic>Salmonella;</jats:italic> therefore, our goal was to exploit iron uptake systems as vaccine targets that could starve this pathogen of iron and thus prevent it from colonizing chickens. One challenge with this approach is that <jats:italic>Salmonella</jats:italic> possesses several iron acquisition proteins that are redundant in function. Therefore, we created nanoparticle‐based plant‐produced vaccine candidates against four different <jats:italic>Salmonella</jats:italic> iron acquisition proteins to create a multivalent vaccine that may be more effective than targeting a single protein. Extracellular antigenic peptides from each of these proteins were fused in tandem to the N‐terminus of the self‐assembling <jats:italic>Brucella</jats:italic> spp. lumazine synthase. These fusion constructs were then expressed in <jats:italic>Nicotiana benthamiana</jats:italic> plants. The resulting recombinant proteins were extracted, purified and characterized. The vaccine candidates administered individually or in combination elicited specific antibodies in mice and bound to the surface of <jats:italic>Salmonella,</jats:italic> indicating their potential in preventing <jats:italic>Salmonella</jats:italic> colonization in poultry.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"37 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148428815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Metabolomics and Selection Signal Analysis Provide Insights Into the Selection for Flavonol Biosynthesis Associated With Lettuce Quality Improvement","authors":"Xiao Yang, Shiwei Wei, Ge Jin, Li Zhang, Ran Du, Yajun Wang, Chengcheng Shen, Tao Huang, Guotao Huo, Jie Peng, Bo Song, Fangyuan Zhang, Victor Hugo Escalona Contreras, Lijun Luo, Yuejian Li, Francisco A. Tomas‐Barberan, Bin Liu, Qichang Yang","doi":"10.1111/pbi.70716","DOIUrl":"https://doi.org/10.1111/pbi.70716","url":null,"abstract":"Lettuce is a critical leafy vegetable consumed worldwide and is a substantial dietary source of health‐promoting compounds. Exploring changes in metabolism during lettuce domestication under artificial selection conditions is important to facilitate further breeding and cultivation for quality improvement. A liquid chromatography‐mass spectrometry‐based metabolomics approach was used to putatively identify 237 metabolites from 40 accessions, of which 130 were identified as metabolite identification level 1. Subsequently, 29 metabolites linked to lettuce quality improvement and their potential associated genes involved in lettuce domestication and differentiation were analysed. Muti‐omics approach showed that metabolites involved in flavonol biosynthesis are the main metabolic distinctions between wild and modern cultivars, which is attributable to the selection signal observed in <jats:italic>LsF3</jats:italic> ′ <jats:italic>H</jats:italic> , a key enzyme involved in the catalysis of flavonoid hydroxylation at the 3′‐position. These findings provide a comprehensive view of quality‐ and flavour‐related metabolite variation in lettuce, reveal the potential for quality improvement associated with flavonol biosynthesis, and offer valuable insights into the genetic basis for improving lettuce flavour and nutrition.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"147 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148428814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}